名称: | pEarleyGate 100 |
---|---|
别称: | pEarleyGate100 |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 蛋白表达 |
片段类型: | ORF,Gateway |
片段物种: | 空载体 |
原核抗性: | Kan |
筛选标记: | Bar |
荧光标记: | |
启动子: | 35S |
复制子: | pVS1 |
拷贝数: | |
感受态: | DB3.1 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGGCAGGATA
TATTGTGGTG
TAAACAAATT
GACGCTTAGA
CAACTTAATA
ACACATTGCG
GACGTTTTTA
ATGTACTGAA
TTAACGCCGA
ATTAATTCGA
GCTCGGATCT
GATAATTTAT
TTGAAAATTC
ATAAGAAAAG
CAAACGTTAC
ATGAATTGAT
GAAACAATAC
AAAGACAGAT
AAAGCCACGC
ACATTTAGGA
TATTGGCCGA
GATTACTGAA
TATTGAGTAA
GATCACGGAA
TTTCTGACAG
GAGCATGTCT
TCAATTCAGC
CCAAATGGCA
GTTGAAATAC
TCAAACCGCC
CCATATGCAG
GAGCGGATCA
TTCATTGTTT
GTTTGGTTGC
CTTTGCCAAC
ATGGGAGTCC
AAGATTCTGC
AGTCAAATCT
CGGTGACGGG
CAGGACCGGA
CGGGGCGGTA
CCGGCAGGCT
GAAGTCCAGC
TGCCAGAAAC
CCACGTCATG
CCAGTTCCCG
TGCTTGAAGC
CGGCCGCCCG
CAGCATGCCG
CGGGGGGCAT
ATCCGAGCGC
CTCGTGCATG
CGCACGCTCG
GGTCGTTGGG
CAGCCCGATG
ACAGCGACCA
CGCTCTTGAA
GCCCTGTGCC
TCCAGGGACT
TCAGCAGGTG
GGTGTAGAGC
GTGGAGCCCA
GTCCCGTCCG
CTGGTGGCGG
GGGGAGACGT
ACACGGTCGA
CTCGGCCGTC
CAGTCGTAGG
CGTTGCGTGC
CTTCCAGGGG
CCCGCGTAGG
CGATGCCGGC
GACCTCGCCG
TCCACCTCGG
CGACGAGCCA
GGGATAGCGC
TCCCGCAGAC
GGACGAGGTC
GTCCGTCCAC
TCCTGCGGTT
CCTGCGGCTC
GGTACGGAAG
TTGACCGTGC
TTGTCTCGAT
GTAGTGGTTG
ACGATGGTGC
AGACCGCCGG
CATGTCCGCC
TCGGTGGCAC
GGCGGATGTC
GGCCGGGCGT
CGTTCTGGGC
TCATCGATTC
GATTTGGTGT
ATCGAGATTG
GTTATGAAAT
TCAGATGCTA
GTGTAATGTA
TTGGTAATTT
GGGAAGATAT
AATAGGAAGC
AAGGCTATTT
ATCCATTTCT
GAAAAGGCGA
AATGGCGTCA
CCGCGAGCGT
CACGCGCATT
CCGTTCTTGC
TGTAAAGCGT
TGTTTGGTAC
ACTTTTGACT
AGCGAGGCTT
GGCGTGTCAG
CGTATCTATT
CAAAAGTCGT
TAATGGCTGC
GGATCAAGAA
AAAGTTGGAA
TAGAAACAGA
ATACCCGCGA
AATTCAGGCC
CGGTTGCCAT
GTCCTACACG
CCGAAATAAA
CGACCAAATT
AGTAGAAAAA
TAAAAACTGA
CTCGGATACT
TACGTCACGT
CTTGCGCACT
GATTTGAAAA
ATCTCAGAAT
TCCAATCCCA
CAAAAATCTG
AGCTTAACAG
CACAGTTGCT
CCTCTCAGAG
CAGAATCGGG
TATTCAACAC
CCTCATATCA
ACTACTACGT
TGTGTATAAC
GGTCCACATG
CCGGTATATA
CGATGACTGG
GGTTGTACAA
AGGCGGCAAC
AAACGGCGTT
CCCGGAGTTG
CACACAAGAA
ATTTGCCACT
ATTACAGAGG
CAAGAGCAGC
AGCTGACGCG
TACACAACAA
GTCAGCAAAC
AGACAGGTTG
AACTTCATCC
CCAAAGGAGA
AGCTCAACTC
AAGCCCAAGA
GCTTTGCTAA
GGCCCTAACA
AGCCCACCAA
AGCAAAAAGC
CCACTGGCTC
ACGCTAGGAA
CCAAAAGGCC
CAGCAGTGAT
CCAGCCCCAA
AAGAGATCTC
CTTTGCCCCG
GAGATTACAA
TGGACGATTT
CCTCTATCTT
TACGATCTAG
GAAGGAAGTT
CGAAGGTGAA
GGTGACGACA
CTATGTTCAC
CACTGATAAT
GAGAAGGTTA
GCCTCTTCAA
TTTCAGAAAG
AATGCTGACC
CACAGATGGT
TAGAGAGGCC
TACGCAGCAG
GTCTCATCAA
GACGATCTAC
CCGAGTAACA
ATCTCCAGGA
GATCAAATAC
CTTCCCAAGA
AGGTTAAAGA
TGCAGTCAAA
AGATTCAGGA
CTAATTGCAT
CAAGAACACA
GAGAAAGACA
TATTTCTCAA
GATCAGAAGT
ACTATTCCAG
TATGGACGAT
TCAAGGCTTG
CTTCATAAAC
CAAGGCAAGT
AATAGAGATT
GGAGTCTCTA
AAAAGGTAGT
TCCTACTGAA
TCTAAGGCCA
TGCATGGAGT
CTAAGATTCA
AATCGAGGAT
CTAACAGAAC
TCGCCGTGAA
GACTGGCGAA
CAGTTCATAC
AGAGTCTTTT
ACGACTCAAT
GACAAGAAGA
AAATCTTCGT
CAACATGGTG
GAGCACGACA
CTCTGGTCTA
CTCCAAAAAT
GTCAAAGATA
CAGTCTCAGA
AGACCAAAGG
GCTATTGAGA
CTTTTCAACA
AAGGATAATT
TCGGGAAACC
TCCTCGGATT
CCATTGCCCA
GCTATCTGTC
ACTTCATCGA
AAGGACAGTA
GAAAAGGAAG
GTGGCTCCTA
CAAATGCCAT
CATTGCGATA
AAGGAAAGGC
TATCATTCAA
GATCTCTCTG
CCGACAGTGG
TCCCAAAGAT
GGACCCCCAC
CCACGAGGAG
CATCGTGGAA
AAAGAAGACG
TTCCAACCAC
GTCTTCAAAG
CAAGTGGATT
GATGTGACAT
CTCCACTGAC
GTAAGGGATG
ACGCACAATC
CCACTATCCT
TCGCAAGACC
CTTCCTCTAT
ATAAGGAAGT
TCATTTCATT
TGGAGAGGAC
ACGCTCGAGA
TCACAAGTTT
GTACAAAAAA
GCTGAACGAG
AAACGTAAAA
TGATATAAAT
ATCAATATAT
TAAATTAGAT
TTTGCATAAA
AAACAGACTA
CATAATACTG
TAAAACACAA
CATATCCAGT
CATATTGGCG
GCCGCATTAG
GCACCCCAGG
CTTTACACTT
TATGCTTCCG
GCTCGTATAA
TGTGTGGATT
TTGAGTTAGG
ATCCGTCGAG
ATTTTCAGGA
GCTAAGGAAG
CTAAAATGGA
GAAAAAAATC
ACTGGATATA
CCACCGTTGA
TATATCCCAA
TGGCATCGTA
AAGAACATTT
TGAGGCATTT
CAGTCAGTTG
CTCAATGTAC
CTATAACCAG
ACCGTTCAGC
TGGATATTAC
GGCCTTTTTA
AAGACCGTAA
AGAAAAATAA
GCACAAGTTT
TATCCGGCCT
TTATTCACAT
TCTTGCCCGC
CTGATGAATG
CTCATCCGGA
ATTCCGTATG
GCAATGAAAG
ACGGTGAGCT
GGTGATATGG
GATAGTGTTC
ACCCTTGTTA
CACCGTTTTC
CATGAGCAAA
CTGAAACGTT
TTCATCGCTC
TGGAGTGAAT
ACCACGACGA
TTTCCGGCAG
TTTCTACACA
TATATTCGCA
AGATGTGGCG
TGTTACGGTG
AAAACCTGGC
CTATTTCCCT
AAAGGGTTTA
TTGAGAATAT
GTTTTTCGTC
TCAGCCAATC
CCTGGGTGAG
TTTCACCAGT
TTTGATTTAA
ACGTGGCCAA
TATGGACAAC
TTCTTCGCCC
CCGTTTTCAC
CATGGGCAAA
TATTATACGC
AAGGCGACAA
GGTGCTGATG
CCGCTGGCGA
TTCAGGTTCA
TCATGCCGTT
TGTGATGGCT
TCCATGTCGG
CAGAATGCTT
AATGAATTAC
AACAGTACTG
CGATGAGTGG
CAGGGCGGGG
CGTAAACGCG
TGGATCCGGC
TTACTAAAAG
CCAGATAACA
GTATGCGTAT
TTGCGCGCTG
ATTTTTGCGG
TATAAGAATA
TATACTGATA
TGTATACCCG
AAGTATGTCA
AAAAGAGGTA
TGCTATGAAG
CAGCGTATTA
CAGTGACAGT
TGACAGCGAC
AGCTATCAGT
TGCTCAAGGC
ATATATGATG
TCAATATCTC
CGGTCTGGTA
AGCACAACCA
TGCAGAATGA
AGCCCGTCGT
CTGCGTGCCG
AACGCTGGAA
AGCGGAAAAT
CAGGAAGGGA
TGGCTGAGGT
CGCCCGGTTT
ATTGAAATGA
ACGGCTCTTT
TGCTGACGAG
AACAGGGGCT
GGTGAAATGC
AGTTTAAGGT
TTACACCTAT
AAAAGAGAGA
GCCGTTATCG
TCTGTTTGTG
GATGTACAGA
GTGATATTAT
TGACACGCCC
GGGCGACGGA
TGGTGATCCC
CCTGGCCAGT
GCACGTCTGC
TGTCAGATAA
AGTCTCCCGT
GAACTTTACC
CGGTGGTGCA
TATCGGGGAT
GAAAGCTGGC
GCATGATGAC
CACCGATATG
GCCAGTGTGC
CGGTCTCCGT
TATCGGGGAA
GAAGTGGCTG
ATCTCAGCCA
CCGCGAAAAT
GACATCAAAA
ACGCCATTAA
CCTGATGTTC
TGGGGAATAT
AAATGTCAGG
CTCCCTTATA
CACAGCCAGT
CTGCAGGTCG
ACCATAGTGA
CTGGATATGT
TGTGTTTTAC
AGCATTATGT
AGTCTGTTTT
TTATGCAAAA
TCTAATTTAA
TATATTGATA
TTTATATCAT
TTTACGTTTC
TCGTTCAGCT
TTCTTGTACA
AAGTGGTGCC
TAGGTGAGTC
TAGAGAGTTA
ATTAAGACCC
GGGACTAGTC
CCTAGAGTCC
TGCTTTAATG
AGATATGCGA
GACGCCTATG
ATCGCATGAT
ATTTGCTTTC
AATTCTGTTG
TGCACGTTGT
AAAAAACCTG
AGCATGTGTA
GCTCAGATCC
TTACCGCCGG
TTTCGGTTCA
TTCTAATGAA
TATATCACCC
GTTACTATCG
TATTTTTATG
AATAATATTC
TCCGTTCAAT
TTACTGATTG
TACCCTACTA
CTTATATGTA
CAATATTAAA
ATGAAAACAA
TATATTGTGC
TGAATAGGTT
TATAGCGACA
TCTATGATAG
AGCGCCACAA
TAACAAACAA
TTGCGTTTTA
TTATTACAAA
TCCAATTTTA
AAAAAAGCGG
CAGAACCGGT
CAAACCTAAA
AGACTGATTA
CATAAATCTT
ATTCAAATTT
CAAAAGTGCC
CCAGGGGCTA
GTATCTACGA
CACACCGAGC
GGCGAACTAA
TAACGCTCAC
TGAAGGGAAC
TCCGGTTCCC
CGCCGGCGCG
CATGGGTGAG
ATTCCTTGAA
GTTGAGTATT
GGCCGTCCGC
TCTACCGAAA
GTTACGGGCA
CCATTCAACC
CGGTCCAGCA
CGGCGGCCGG
GTAACCGACT
TGCTGCCCCG
AGAATTATGC
AGCATTTTTT
TGGTGTATGT
GGGCCCCAAA
TGAAGTGCAG
GTCAAACCTT
GACAGTGACG
ACAAATCGTT
GGGCGGGTCC
AGGGCGAATT
TTGCGACAAC
ATGTCGAGGC
TCAGCAGGAC
CTGCAGGCAT
GCAAGCTTGG
CACTGGCCGT
CGTTTTACAA
CGTCGTGACT
GGGAAAACCC
TGGCGTTACC
CAACTTAATC
GCCTTGCAGC
ACATCCCCCT
TTCGCCAGCT
GGCGTAATAG
CGAAGAGGCC
CGCACCGATC
GCCCTTCCCA
ACAGTTGCGC
AGCCTGAATG
GCGAATGCTA
GAGCAGCTTG
AGCTTGGATC
AGATTGTCGT
TTCCCGCCTT
CAGTTTAAAC
TATCAGTGTT
TGACAGGATA
TATTGGCGGG
TAAACCTAAG
AGAAAAGAGC
GTTTATTAGA
ATAACGGATA
TTTAAAAGGG
CGTGAAAAGG
TTTATCCGTT
CGTCCATTTG
TATGTGCATG
CCAACCACAG
GGTTCCCCTC
GGGATCAAAG
TACTTTGATC
CAACCCCTCC
GCTGCTATAG
TGCAGTCGGC
TTCTGACGTT
CAGTGCAGCC
GTCTTCTGAA
AACGACATGT
CGCACAAGTC
CTAAGTTACG
CGACAGGCTG
CCGCCCTGCC
CTTTTCCTGG
CGTTTTCTTG
TCGCGTGTTT
TAGTCGCATA
AAGTAGAATA
CTTGCGACTA
GAACCGGAGA
CATTACGCCA
TGAACAAGAG
CGCCGCCGCT
GGCCTGCTGG
GCTATGCCCG
CGTCAGCACC
GACGACCAGG
ACTTGACCAA
CCAACGGGCC
GAACTGCACG
CGGCCGGCTG
CACCAAGCTG
TTTTCCGAGA
AGATCACCGG
CACCAGGCGC
GACCGCCCGG
AGCTGGCCAG
GATGCTTGAC
CACCTACGCC
CTGGCGACGT
TGTGACAGTG
ACCAGGCTAG
ACCGCCTGGC
CCGCAGCACC
CGCGACCTAC
TGGACATTGC
CGAGCGCATC
CAGGAGGCCG
GCGCGGGCCT
GCGTAGCCTG
GCAGAGCCGT
GGGCCGACAC
CACCACGCCG
GCCGGCCGCA
TGGTGTTGAC
CGTGTTCGCC
GGCATTGCCG
AGTTCGAGCG
TTCCCTAATC
ATCGACCGCA
CCCGGAGCGG
GCGCGAGGCC
GCCAAGGCCC
GAGGCGTGAA
GTTTGGCCCC
CGCCCTACCC
TCACCCCGGC
ACAGATCGCG
CACGCCCGCG
AGCTGATCGA
CCAGGAAGGC
CGCACCGTGA
AAGAGGCGGC
TGCACTGCTT
GGCGTGCATC
GCTCGACCCT
GTACCGCGCA
CTTGAGCGCA
GCGAGGAAGT
GACGCCCACC
GAGGCCAGGC
GGCGCGGTGC
CTTCCGTGAG
GACGCATTGA
CCGAGGCCGA
CGCCCTGGCG
GCCGCCGAGA
ATGAACGCCA
AGAGGAACAA
GCATGAAACC
GCACCAGGAC
GGCCAGGACG
AACCGTTTTT
CATTACCGAA
GAGATCGAGG
CGGAGATGAT
CGCGGCCGGG
TACGTGTTCG
AGCCGCCCGC
GCACGTCTCA
ACCGTGCGGC
TGCATGAAAT
CCTGGCCGGT
TTGTCTGATG
CCAAGCTGGC
GGCCTGGCCG
GCCAGCTTGG
CCGCTGAAGA
AACCGAGCGC
CGCCGTCTAA
AAAGGTGATG
TGTATTTGAG
TAAAACAGCT
TGCGTCATGC
GGTCGCTGCG
TATATGATGC
GATGAGTAAA
TAAACAAATA
CGCAAGGGGA
ACGCATGAAG
GTTATCGCTG
TACTTAACCA
GAAAGGCGGG
TCAGGCAAGA
CGACCATCGC
AACCCATCTA
GCCCGCGCCC
TGCAACTCGC
CGGGGCCGAT
GTTCTGTTAG
TCGATTCCGA
TCCCCAGGGC
AGTGCCCGCG
ATTGGGCGGC
CGTGCGGGAA
GATCAACCGC
TAACCGTTGT
CGGCATCGAC
CGCCCGACGA
TTGACCGCGA
CGTGAAGGCC
ATCGGCCGGC
GCGACTTCGT
AGTGATCGAC
GGAGCGCCCC
AGGCGGCGGA
CTTGGCTGTG
TCCGCGATCA
AGGCAGCCGA
CTTCGTGCTG
ATTCCGGTGC
AGCCAAGCCC
TTACGACATA
TGGGCCACCG
CCGACCTGGT
GGAGCTGGTT
AAGCAGCGCA
TTGAGGTCAC
GGATGGAAGG
CTACAAGCGG
CCTTTGTCGT
GTCGCGGGCG
ATCAAAGGCA
CGCGCATCGG
CGGTGAGGTT
GCCGAGGCGC
TGGCCGGGTA
CGAGCTGCCC
ATTCTTGAGT
CCCGTATCAC
GCAGCGCGTG
AGCTACCCAG
GCACTGCCGC
CGCCGGCACA
ACCGTTCTTG
AATCAGAACC
CGAGGGCGAC
GCTGCCCGCG
AGGTCCAGGC
GCTGGCCGCT
GAAATTAAAT
CAAAACTCAT
TTGAGTTAAT
GAGGTAAAGA
GAAAATGAGC
AAAAGCACAA
ACACGCTAAG
TGCCGGCCGT
CCGAGCGCAC
GCAGCAGCAA
GGCTGCAACG
TTGGCCAGCC
TGGCAGACAC
GCCAGCCATG
AAGCGGGTCA
ACTTTCAGTT
GCCGGCGGAG
GATCACACCA
AGCTGAAGAT
GTACGCGGTA
CGCCAAGGCA
AGACCATTAC
CGAGCTGCTA
TCTGAATACA
TCGCGCAGCT
ACCAGAGTAA
ATGAGCAAAT
GAATAAATGA
GTAGATGAAT
TTTAGCGGCT
AAAGGAGGCG
GCATGGAAAA
TCAAGAACAA
CCAGGCACCG
ACGCCGTGGA
ATGCCCCATG
TGTGGAGGAA
CGGGCGGTTG
GCCAGGCGTA
AGCGGCTGGG
TTGTCTGCCG
GCCCTGCAAT
GGCACTGGAA
CCCCCAAGCC
CGAGGAATCG
GCGTGAGCGG
TCGCAAACCA
TCCGGCCCGG
TACAAATCGG
CGCGGCGCTG
GGTGATGACC
TGGTGGAGAA
GTTGAAGGCC
GCGCAGGCCG
CCCAGCGGCA
ACGCATCGAG
GCAGAAGCAC
GCCCCGGTGA
ATCGTGGCAA
GCGGCCGCTG
ATCGAATCCG
CAAAGAATCC
CGGCAACCGC
CGGCAGCCGG
TGCGCCGTCG
ATTAGGAAGC
CGCCCAAGGG
CGACGAGCAA
CCAGATTTTT
TCGTTCCGAT
GCTCTATGAC
GTGGGCACCC
GCGATAGTCG
CAGCATCATG
GACGTGGCCG
TTTTCCGTCT
GTCGAAGCGT
GACCGACGAG
CTGGCGAGGT
GATCCGCTAC
GAGCTTCCAG
ACGGGCACGT
AGAGGTTTCC
GCAGGGCCGG
CCGGCATGGC
CAGTGTGTGG
GATTACGACC
TGGTACTGAT
GGCGGTTTCC
CATCTAACCG
AATCCATGAA
CCGATACCGG
GAAGGGAAGG
GAGACAAGCC
CGGCCGCGTG
TTCCGTCCAC
ACGTTGCGGA
CGTACTCAAG
TTCTGCCGGC
GAGCCGATGG
CGGAAAGCAG
AAAGACGACC
TGGTAGAAAC
CTGCATTCGG
TTAAACACCA
CGCACGTTGC
CATGCAGCGT
ACGAAGAAGG
CCAAGAACGG
CCGCCTGGTG
ACGGTATCCG
AGGGTGAAGC
CTTGATTAGC
CGCTACAAGA
TCGTAAAGAG
CGAAACCGGG
CGGCCGGAGT
ACATCGAGAT
CGAGCTAGCT
GATTGGATGT
ACCGCGAGAT
CACAGAAGGC
AAGAACCCGG
ACGTGCTGAC
GGTTCACCCC
GATTACTTTT
TGATCGATCC
CGGCATCGGC
CGTTTTCTCT
ACCGCCTGGC
ACGCCGCGCC
GCAGGCAAGG
CAGAAGCCAG
ATGGTTGTTC
AAGACGATCT
ACGAACGCAG
TGGCAGCGCC
GGAGAGTTCA
AGAAGTTCTG
TTTCACCGTG
CGCAAGCTGA
TCGGGTCAAA
TGACCTGCCG
GAGTACGATT
TGAAGGAGGA
GGCGGGGCAG
GCTGGCCCGA
TCCTAGTCAT
GCGCTACCGC
AACCTGATCG
AGGGCGAAGC
ATCCGCCGGT
TCCTAATGTA
CGGAGCAGAT
GCTAGGGCAA
ATTGCCCTAG
CAGGGGAAAA
AGGTCGAAAA
GGTCTCTTTC
CTGTGGATAG
CACGTACATT
GGGAACCCAA
AGCCGTACAT
TGGGAACCGG
AACCCGTACA
TTGGGAACCC
AAAGCCGTAC
ATTGGGAACC
GGTCACACAT
GTAAGTGACT
GATATAAAAG
AGAAAAAAGG
CGATTTTTCC
GCCTAAAACT
CTTTAAAACT
TATTAAAACT
CTTAAAACCC
GCCTGGCCTG
TGCATAACTG
TCTGGCCAGC
GCACAGCCGA
AGAGCTGCAA
AAAGCGCCTA
CCCTTCGGTC
GCTGCGCTCC
CTACGCCCCG
CCGCTTCGCG
TCGGCCTATC
GCGGCCGCTG
GCCGCTCAAA
AATGGCTGGC
CTACGGCCAG
GCAATCTACC
AGGGCGCGGA
CAAGCCGCGC
CGTCGCCACT
CGACCGCCGG
CGCCCACATC
AAGGCACCCT
GCCTCGCGCG
TTTCGGTGAT
GACGGTGAAA
ACCTCTGACA
CATGCAGCTC
CCGGAGACGG
TCACAGCTTG
TCTGTAAGCG
GATGCCGGGA
GCAGACAAGC
CCGTCAGGGC
GCGTCAGCGG
GTGTTGGCGG
GTGTCGGGGC
GCAGCCATGA
CCCAGTCACG
TAGCGATAGC
GGAGTGTATA
CTGGCTTAAC
TATGCGGCAT
CAGAGCAGAT
TGTACTGAGA
GTGCACCATA
TGCGGTGTGA
AATACCGCAC
AGATGCGTAA
GGAGAAAATA
CCGCATCAGG
CGCTCTTCCG
CTTCCTCGCT
CACTGACTCG
CTGCGCTCGG
TCGTTCGGCT
GCGGCGAGCG
GTATCAGCTC
ACTCAAAGGC
GGTAATACGG
TTATCCACAG
AATCAGGGGA
TAACGCAGGA
AAGAACATGT
GAGCAAAAGG
CCAGCAAAAG
GCCAGGAACC
GTAAAAAGGC
CGCGTTGCTG
GCGTTTTTCC
ATAGGCTCCG
CCCCCCTGAC
GAGCATCACA
AAAATCGACG
CTCAAGTCAG
AGGTGGCGAA
ACCCGACAGG
ACTATAAAGA
TACCAGGCGT
TTCCCCCTGG
AAGCTCCCTC
GTGCGCTCTC
CTGTTCCGAC
CCTGCCGCTT
ACCGGATACC
TGTCCGCCTT
TCTCCCTTCG
GGAAGCGTGG
CGCTTTCTCA
TAGCTCACGC
TGTAGGTATC
TCAGTTCGGT
GTAGGTCGTT
CGCTCCAAGC
TGGGCTGTGT
GCACGAACCC
CCCGTTCAGC
CCGACCGCTG
CGCCTTATCC
GGTAACTATC
GTCTTGAGTC
CAACCCGGTA
AGACACGACT
TATCGCCACT
GGCAGCAGCC
ACTGGTAACA
GGATTAGCAG
AGCGAGGTAT
GTAGGCGGTG
CTACAGAGTT
CTTGAAGTGG
TGGCCTAACT
ACGGCTACAC
TAGAAGGACA
GTATTTGGTA
TCTGCGCTCT
GCTGAAGCCA
GTTACCTTCG
GAAAAAGAGT
TGGTAGCTCT
TGATCCGGCA
AACAAACCAC
CGCTGGTAGC
GGTGGTTTTT
TTGTTTGCAA
GCAGCAGATT
ACGCGCAGAA
AAAAAGGATC
TCAAGAAGAT
CCTTTGATCT
TTTCTACGGG
GTCTGACGCT
CAGTGGAACG
AAAACTCACG
TTAAGGGATT
TTGGTCATGC
ATTCTAGGTA
CTAAAACAAT
TCATCCAGTA
AAATATAATA
TTTTATTTTC
TCCCAATCAG
GCTTGATCCC
CAGTAAGTCA
AAAAATAGCT
CGACATACTG
TTCTTCCCCG
ATATCCTCCC
TGATCGACCG
GACGCAGAAG
GCAATGTCAT
ACCACTTGTC
CGCCCTGCCG
CTTCTCCCAA
GATCAATAAA
GCCACTTACT
TTGCCATCTT
TCACAAAGAT
GTTGCTGTCT
CCCAGGTCGC
CGTGGGAAAA
GACAAGTTCC
TCTTCGGGCT
TTTCCGTCTT
TAAAAAATCA
TACAGCTCGC
GCGGATCTTT
AAATGGAGTG
TCTTCTTCCC
AGTTTTCGCA
ATCCACATCG
GCCAGATCGT
TATTCAGTAA
GTAATCCAAT
TCGGCTAAGC
GGCTGTCTAA
GCTATTCGTA
TAGGGACAAT
CCGATATGTC
GATGGAGTGA
AAGAGCCTGA
TGCACTCCGC
ATACAGCTCG
ATAATCTTTT
CAGGGCTTTG
TTCATCTTCA
TACTCTTCCG
AGCAAAGGAC
GCCATCGGCC
TCACTCATGA
GCAGATTGCT
CCAGCCATCA
TGCCGTTCAA
AGTGCAGGAC
CTTTGGAACA
GGCAGCTTTC
CTTCCAGCCA
TAGCATCATG
TCCTTTTCCC
GTTCCACATC
ATAGGTGGTC
CCTTTATACC
GGCTGTCCGT
CATTTTTAAA
TATAGGTTTT
CATTTTCTCC
CACCAGCTTA
TATACCTTAG
CAGGAGACAT
TCCTTCCGTA
TCTTTTACGC
AGCGGTATTT
TTCGATCAGT
TTTTTCAATT
CCGGTGATAT
TCTCATTTTA
GCCATTTATT
ATTTCCTTCC
TCTTTTCTAC
AGTATTTAAA
GATACCCCAA
GAAGCTAATT
ATAACAAGAC
GAACTCCAAT
TCACTGTTCC
TTGCATTCTA
AAACCTTAAA
TACCAGAAAA
CAGCTTTTTC
AAAGTTGTTT
TCAAAGTTGG
CGTATAACAT
AGTATCGACG
GAGCCGATTT
TGAAACCGCG
GTGATCACAG
GCAGCAACGC
TCTGTCATCG
TTACAATCAA
CATGCTACCC
TCCGCGAGAT
CATCCGTGTT
TCAAACCCGG
CAGCTTAGTT
GCCGTTCTTC
CGAATAGCAT
CGGTAACATG
AGCAAAGTCT
GCCGCCTTAC
AACGGCTCTC
CCGCTGACGC
CGTCCCGGAC
TGATGGGCTG
CCTGTATCGA
GTGGTGATTT
TGTGCCGAGC
TGCCGGTCGG
GGAGCTGTTG
GCTGGCTGG