产品货号：P2927
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pAc-sgRNA-Cas9 |
|---|---|
| 别称: | Plasmid#49330 |
| 基因长: | |
| 质粒宿主： | 昆虫细胞 |
| 质粒用途： | 基因编辑 |
| 片段类型： | CRISPR |
| 片段物种： | 空载体 |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | |
| 启动子: | dU6-2 |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | |
| 正向引物: | |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
CATTTTTCCA
GATCTAAAAA
AGCACCGACT
CGGTGCCACT
TTTTCAAGTT
GATAACGGAC
TAGCCTTATT
TTAACTTGCT
ATTTCTAGCT
CTAAAACGGA
AGAGCTCGCT
CTTCCGAAGT
ATTGAGGAAA
ACATACCTAT
ATAAATGATC
AACATCAGGA
AAGAGCAGTT
GAGAATTATA
AGAATTGGCA
AATGGTCTTA
AGAACCCTCT
GCTTAAGATT
TTCAAAATTT
CCTTTAGAAT
CAAAGTGTCC
TATTGTTCGT
TTGTTGAAAA
CACAGTTCGA
TTTATTGACT
ATAATAAATT
GATAGTTTTA
AATATAGAGG
CACGACTAAG
AGAGTTTGGT
TTTGTTTTGG
TTAGGGACCA
AAAAAAGTAT
ACATAACGAA
TAAAAAAGGA
TTTAAGTGCA
AATGGTAAAA
AAGTGTCGAG
TTTTTCTTGA
GTTGATTGTG
CTGTAATGAG
ACTCTGCATT
CGGCATTTGA
CTCGGCTTTT
CCTACTCGTG
CCGTATTTCA
GGCTGCAAGT
CGAACAGATC
TCGCTGCCTG
TTATGTGGCC
CACAAACCAA
GACACGTTTT
ATGGCCATTA
AAGCTGGCTG
ATCGTCGCCA
AACACCAAAT
ACATAATGAA
TATGTACACA
TTCGAGAAAG
AAGCGATCAA
AGAAGCGTCT
TCGGGCGGAG
TAGGAGAATG
CGGAGGAGAA
GGAGAACGAG
CTGATCTAGT
ATCTCTCCAC
AATCCAATGC
CAACTGACCA
ACTGGCCATA
TTCGGAGCAA
TTTGAAGCCA
ATTTCCATCG
CCTGGCGATC
GCTCCATTCT
TGGCTATATG
TTTTTCACCG
TTACCCGGGG
CCATTTTCAA
AGACTCGTCG
GCAAGATAAG
ATTGTGTCAC
TCGCTGTCTC
TCTTCATTTG
TCGAAGAATG
CTGAGGAATT
TCGCGATGAC
GTCGGCGAGT
ATTTTGAAGA
ATGAGAATAA
TTTGTATTTA
TACGAAAATC
AGTTAGTGGA
ATTTTCTACA
AAAACATGTT
ATCTATAGAT
AATTTTGTTG
CAAAATATGT
TGACTATGAC
AAAGATTGTA
TGTATATACC
TTTAATGTAT
TCTCATTTTC
TTATGTATTT
ATAATGGCAA
TGATGATACT
GATGATATTT
TAAGATGATG
CCAGACCAAA
AGGCTTGAAT
TTCTGCGTCT
TTTGCCGAAC
GCAGTGCATG
TGCAATTGTT
GTTTTTTGGA
ATATTCAATT
TTCGGACTGT
CCGCTTTGAT
TTCAGTTTCT
TGGCTTATTC
AAAAAGCAAA
GTAAAGCCAA
AAAAGCGAGA
TGGCAATACC
AAATGCGGCA
AAACGGTAGT
GGAAGGAAAG
GGGTGCGGGG
CAGCGGAAGG
AAGGGTGGGG
CGGGGCGTGG
CGGGGTCTGT
GGCTGGGCGC
GACGTCACCG
ACGTTGGAGC
CACTCCTTTG
ACCATGTGTG
CGTGTGTGTA
TTATTCGTGT
CTCGCCACTC
GCCGGTTGTT
TTTTTCTTTT
TATGCTGCGC
TCTCTCTAGC
GCCATCTCGC
TTACGCATGC
TCAACGCACC
GCATGTTGCC
GTTTCCTTTT
ATGCGTCATT
TTGGCTCGAA
ATAGGCAATT
ATTTAAACAA
AGATTAGTCA
ACGAAAACGC
TAAAATAAAT
AAGTCTACAA
TATGGTTACT
TATTGCCATG
TGTGTGCAGC
CAACGATAGC
AACAAAAGCA
ACAACACAGG
TGGCTTTCCC
TCTTTCACTT
TTTGTTTGCA
AGCCGCGTGC
GAGCAAGACG
GCACGACCGG
CAAACGCAAT
TACGCTGACA
AAGAGCAGAC
GAAGTTTTGG
CGAAAAACAT
CAAGGCGCCT
GATACGAATG
CATTTGCAAT
AACAATTGCG
ATATTTAATA
TTGTTTATGA
AGCTGTTTGA
CTTCAAAACA
CACAAAAAAA
AAAATAAAAC
AAATTATTTG
AAAGAGAATT
AGGAATCGGA
CGCTTATCGT
TAGGGTAACA
ACAAGAAATG
CTTACTGAGT
CACAGCCTCT
GGAAAACTGC
CGCAAGCCAG
AGAGAGAGAG
AAAAAGAGGG
AGAGCAGCTT
AGACCGCATG
TGCTTGTGTG
TGAGGCGTCT
CTCTCTTCGT
CTCTGTTGCG
CAAACGCATA
GACTGCACTG
AAAAAATCGA
TTACCTATTT
TTTATGAATG
AATATTTGCA
CTATTACTAT
TCAAAACTAT
TAAGATAGCA
ATCACATTCA
ATAGCCAAAT
ACTATACCAC
CTGAGCGATG
CAACGAAATG
ATCAATTTGA
GCAAAAATGC
TGCATATTTA
GGACGGCATC
ATTATAGAAA
TGCTTCTTGC
TGTGTACTTT
TCTCTCGTCT
GGCAGCTGTT
TCGCCGTTAT
TGTTAAAACC
GGCTTAAGTT
AGGTGTGTTT
TCTACGACTA
GTGAATGCCC
TACTAGAAGA
TGTGTGTTGC
ACAAAATGTC
CCTGGAATAA
CCAATTTGAA
GTGCAGATAG
CAGTAAACGT
AAGCTAATAT
GAATATTATT
TAACTGTAAT
GTTTTAATAT
CGCTGGACAT
TACTAATAAA
CCCACTATAA
ACACATGTAC
ATATGTATGT
TTTGGCATAC
AATGAGTAGT
TGGGGAAAAA
ATGTGTAAAA
GCACCGTGAC
CATCACAGCA
TAAAGATAAC
CAGCTGAAGT
ATCGAATATG
AGTAACCCCC
AAATTGAATC
ACATGCCGCA
ACTGATAGGA
CCCATGGAAG
TACACTCTTC
ATGGCGATAT
ACAAGACACA
CACAAGCACG
AACACCCAGT
TGCGGAGGAA
ATTCTCCGTA
AATGAAAACC
CAATCGGCGA
ACAATTCATA
CCCATATATG
GTAAAAGTTT
TGAACGCGAC
TTGAGAGCGG
AGAGCATTGC
GGCTGATAAG
GTTTTAGCGC
TAAGCGGGCT
TTATAAAACG
GGCTGCGGGA
CCAGTTTTCA
TATCACTACC
GTTTGAGTTC
TTGTGCTGTG
TGGATACTCC
TCCCGACACA
AAGCCGCTCC
ATCAGCCAGC
AGTCGTCTAA
TCCAGAGACC
CCGGATCGGG
GTACCTACTA
GTCCAGTGTG
GTGGAATTCA
TGGACTATAA
GGACCACGAC
GGAGACTACA
AGGATCATGA
TATTGATTAC
AAAGACGATG
ACGATAAGAT
GGCCCCAAAG
AAGAAGCGGA
AGGTCGGTAT
CCACGGAGTC
CCAGCAGCCG
ACAAGAAGTA
CAGCATCGGC
CTGGACATCG
GCACCAACTC
TGTGGGCTGG
GCCGTGATCA
CCGACGAGTA
CAAGGTGCCC
AGCAAGAAAT
TCAAGGTGCT
GGGCAACACC
GACCGGCACA
GCATCAAGAA
GAACCTGATC
GGAGCCCTGC
TGTTCGACAG
CGGCGAAACA
GCCGAGGCCA
CCCGGCTGAA
GAGAACCGCC
AGAAGAAGAT
ACACCAGACG
GAAGAACCGG
ATCTGCTATC
TGCAAGAGAT
CTTCAGCAAC
GAGATGGCCA
AGGTGGACGA
CAGCTTCTTC
CACAGACTGG
AAGAGTCCTT
CCTGGTGGAA
GAGGATAAGA
AGCACGAGCG
GCACCCCATC
TTCGGCAACA
TCGTGGACGA
GGTGGCCTAC
CACGAGAAGT
ACCCCACCAT
CTACCACCTG
AGAAAGAAAC
TGGTGGACAG
CACCGACAAG
GCCGACCTGC
GGCTGATCTA
TCTGGCCCTG
GCCCACATGA
TCAAGTTCCG
GGGCCACTTC
CTGATCGAGG
GCGACCTGAA
CCCCGACAAC
AGCGACGTGG
ACAAGCTGTT
CATCCAGCTG
GTGCAGACCT
ACAACCAGCT
GTTCGAGGAA
AACCCCATCA
ACGCCAGCGG
CGTGGACGCC
AAGGCCATCC
TGTCTGCCAG
ACTGAGCAAG
AGCAGACGGC
TGGAAAATCT
GATCGCCCAG
CTGCCCGGCG
AGAAGAAGAA
TGGCCTGTTC
GGAAACCTGA
TTGCCCTGAG
CCTGGGCCTG
ACCCCCAACT
TCAAGAGCAA
CTTCGACCTG
GCCGAGGATG
CCAAACTGCA
GCTGAGCAAG
GACACCTACG
ACGACGACCT
GGACAACCTG
CTGGCCCAGA
TCGGCGACCA
GTACGCCGAC
CTGTTTCTGG
CCGCCAAGAA
CCTGTCCGAC
GCCATCCTGC
TGAGCGACAT
CCTGAGAGTG
AACACCGAGA
TCACCAAGGC
CCCCCTGAGC
GCCTCTATGA
TCAAGAGATA
CGACGAGCAC
CACCAGGACC
TGACCCTGCT
GAAAGCTCTC
GTGCGGCAGC
AGCTGCCTGA
GAAGTACAAA
GAGATTTTCT
TCGACCAGAG
CAAGAACGGC
TACGCCGGCT
ACATTGACGG
CGGAGCCAGC
CAGGAAGAGT
TCTACAAGTT
CATCAAGCCC
ATCCTGGAAA
AGATGGACGG
CACCGAGGAA
CTGCTCGTGA
AGCTGAACAG
AGAGGACCTG
CTGCGGAAGC
AGCGGACCTT
CGACAACGGC
AGCATCCCCC
ACCAGATCCA
CCTGGGAGAG
CTGCACGCCA
TTCTGCGGCG
GCAGGAAGAT
TTTTACCCAT
TCCTGAAGGA
CAACCGGGAA
AAGATCGAGA
AGATCCTGAC
CTTCCGCATC
CCCTACTACG
TGGGCCCTCT
GGCCAGGGGA
AACAGCAGAT
TCGCCTGGAT
GACCAGAAAG
AGCGAGGAAA
CCATCACCCC
CTGGAACTTC
GAGGAAGTGG
TGGACAAGGG
CGCTTCCGCC
CAGAGCTTCA
TCGAGCGGAT
GACCAACTTC
GATAAGAACC
TGCCCAACGA
GAAGGTGCTG
CCCAAGCACA
GCCTGCTGTA
CGAGTACTTC
ACCGTGTATA
ACGAGCTGAC
CAAAGTGAAA
TACGTGACCG
AGGGAATGAG
AAAGCCCGCC
TTCCTGAGCG
GCGAGCAGAA
AAAGGCCATC
GTGGACCTGC
TGTTCAAGAC
CAACCGGAAA
GTGACCGTGA
AGCAGCTGAA
AGAGGACTAC
TTCAAGAAAA
TCGAGTGCTT
CGACTCCGTG
GAAATCTCCG
GCGTGGAAGA
TCGGTTCAAC
GCCTCCCTGG
GCACATACCA
CGATCTGCTG
AAAATTATCA
AGGACAAGGA
CTTCCTGGAC
AATGAGGAAA
ACGAGGACAT
TCTGGAAGAT
ATCGTGCTGA
CCCTGACACT
GTTTGAGGAC
AGAGAGATGA
TCGAGGAACG
GCTGAAAACC
TATGCCCACC
TGTTCGACGA
CAAAGTGATG
AAGCAGCTGA
AGCGGCGGAG
ATACACCGGC
TGGGGCAGGC
TGAGCCGGAA
GCTGATCAAC
GGCATCCGGG
ACAAGCAGTC
CGGCAAGACA
ATCCTGGATT
TCCTGAAGTC
CGACGGCTTC
GCCAACAGAA
ACTTCATGCA
GCTGATCCAC
GACGACAGCC
TGACCTTTAA
AGAGGACATC
CAGAAAGCCC
AGGTGTCCGG
CCAGGGCGAT
AGCCTGCACG
AGCACATTGC
CAATCTGGCC
GGCAGCCCCG
CCATTAAGAA
GGGCATCCTG
CAGACAGTGA
AGGTGGTGGA
CGAGCTCGTG
AAAGTGATGG
GCCGGCACAA
GCCCGAGAAC
ATCGTGATCG
AAATGGCCAG
AGAGAACCAG
ACCACCCAGA
AGGGACAGAA
GAACAGCCGC
GAGAGAATGA
AGCGGATCGA
AGAGGGCATC
AAAGAGCTGG
GCAGCCAGAT
CCTGAAAGAA
CACCCCGTGG
AAAACACCCA
GCTGCAGAAC
GAGAAGCTGT
ACCTGTACTA
CCTGCAGAAT
GGGCGGGATA
TGTACGTGGA
CCAGGAACTG
GACATCAACC
GGCTGTCCGA
CTACGATGTG
GACCATATCG
TGCCTCAGAG
CTTTCTGAAG
GACGACTCCA
TCGACAACAA
GGTGCTGACC
AGAAGCGACA
AGAACCGGGG
CAAGAGCGAC
AACGTGCCCT
CCGAAGAGGT
CGTGAAGAAG
ATGAAGAACT
ACTGGCGGCA
GCTGCTGAAC
GCCAAGCTGA
TTACCCAGAG
AAAGTTCGAC
AATCTGACCA
AGGCCGAGAG
AGGCGGCCTG
AGCGAACTGG
ATAAGGCCGG
CTTCATCAAG
AGACAGCTGG
TGGAAACCCG
GCAGATCACA
AAGCACGTGG
CACAGATCCT
GGACTCCCGG
ATGAACACTA
AGTACGACGA
GAATGACAAG
CTGATCCGGG
AAGTGAAAGT
GATCACCCTG
AAGTCCAAGC
TGGTGTCCGA
TTTCCGGAAG
GATTTCCAGT
TTTACAAAGT
GCGCGAGATC
AACAACTACC
ACCACGCCCA
CGACGCCTAC
CTGAACGCCG
TCGTGGGAAC
CGCCCTGATC
AAAAAGTACC
CTAAGCTGGA
AAGCGAGTTC
GTGTACGGCG
ACTACAAGGT
GTACGACGTG
CGGAAGATGA
TCGCCAAGAG
CGAGCAGGAA
ATCGGCAAGG
CTACCGCCAA
GTACTTCTTC
TACAGCAACA
TCATGAACTT
TTTCAAGACC
GAGATTACCC
TGGCCAACGG
CGAGATCCGG
AAGCGGCCTC
TGATCGAGAC
AAACGGCGAA
ACCGGGGAGA
TCGTGTGGGA
TAAGGGCCGG
GATTTTGCCA
CCGTGCGGAA
AGTGCTGAGC
ATGCCCCAAG
TGAATATCGT
GAAAAAGACC
GAGGTGCAGA
CAGGCGGCTT
CAGCAAAGAG
TCTATCCTGC
CCAAGAGGAA
CAGCGATAAG
CTGATCGCCA
GAAAGAAGGA
CTGGGACCCT
AAGAAGTACG
GCGGCTTCGA
CAGCCCCACC
GTGGCCTATT
CTGTGCTGGT
GGTGGCCAAA
GTGGAAAAGG
GCAAGTCCAA
GAAACTGAAG
AGTGTGAAAG
AGCTGCTGGG
GATCACCATC
ATGGAAAGAA
GCAGCTTCGA
GAAGAATCCC
ATCGACTTTC
TGGAAGCCAA
GGGCTACAAA
GAAGTGAAAA
AGGACCTGAT
CATCAAGCTG
CCTAAGTACT
CCCTGTTCGA
GCTGGAAAAC
GGCCGGAAGA
GAATGCTGGC
CTCTGCCGGC
GAACTGCAGA
AGGGAAACGA
ACTGGCCCTG
CCCTCCAAAT
ATGTGAACTT
CCTGTACCTG
GCCAGCCACT
ATGAGAAGCT
GAAGGGCTCC
CCCGAGGATA
ATGAGCAGAA
ACAGCTGTTT
GTGGAACAGC
ACAAGCACTA
CCTGGACGAG
ATCATCGAGC
AGATCAGCGA
GTTCTCCAAG
AGAGTGATCC
TGGCCGACGC
TAATCTGGAC
AAAGTGCTGT
CCGCCTACAA
CAAGCACCGG
GATAAGCCCA
TCAGAGAGCA
GGCCGAGAAT
ATCATCCACC
TGTTTACCCT
GACCAATCTG
GGAGCCCCTG
CCGCCTTCAA
GTACTTTGAC
ACCACCATCG
ACCGGAAGAG
GTACACCAGC
ACCAAAGAGG
TGCTGGACGC
CACCCTGATC
CACCAGAGCA
TCACCGGCCT
GTACGAGACA
CGGATCGACC
TGTCTCAGCT
GGGAGGCGAC
AAAAGGCCGG
CGGCCACGAA
AAAGGCCGGC
CAGGCAAAAA
AGAAAAAGGG
ATCAGGCAAG
CTTGAGGGCA
GAGGAAGTCT
TCTAACATGC
GGTGACGTGG
AGGAGAATCC
CGGCCCTGCT
AGCGGTAGCG
GCAGCGGTAG
CATGACCGAG
TACAAGCCCA
CGGTGCGCCT
CGCCACCCGC
GACGACGTCC
CCCGGGCCGT
ACGCACCCTC
GCCGCCGCGT
TCGCCGACTA
CCCCGCCACG
CGCCACACCG
TCGACCCGGA
CCGCCACATC
GAGCGGGTCA
CCGAGCTGCA
AGAACTCTTC
CTCACGCGCG
TCGGGCTCGA
CATCGGCAAG
GTGTGGGTCG
CGGACGACGG
CGCCGCGGTG
GCGGTCTGGA
CCACGCCGGA
GAGCGTCGAA
GCGGGGGCGG
TGTTCGCCGA
GATCGGCCCG
CGCATGGCCG
AGTTGAGCGG
TTCCCGGCTG
GCCGCGCAGC
AACAGATGGA
AGGCCTCCTG
GCGCCGCACC
GGCCCAAGGA
GCCCGCGTGG
TTCCTGGCCA
CCGTCGGCGT
CTCGCCCGAC
CACCAGGGCA
AGGGTCTGGG
CAGCGCCGTC
GTGCTCCCCG
GAGTGGAGGC
GGCCGAGCGC
GCCGGGGTGC
CCGCCTTCCT
GGAGACCTCC
GCGCCCCGCA
ACCTCCCCTT
CTACGAGCGG
CTCGGCTTCA
CCGTCACCGC
CGACGTCGAG
GTGCCCGAAG
GACCGCGCAC
CTGGTGCATG
ACCCGCAAGC
CCGGTGCCTG
ACTCGAGGGA
TCCAGACATG
ATAAGATACA
TTGATGAGTT
TGGACAAACC
ACAACTAGAA
TGCAGTGAAA
AAAATGCTTT
ATTTGTGAAA
TTTGTGATGC
TATTGCTTTA
TTTGTAACCA
TTATAAGCTG
CAATAAACAA
GTTAACAACA
ACAATTGCAT
TCATTTTATG
TTTCAGGTTC
AGGGGGAGGT
GTGGGAGGTT
TTTTAAAGCA
AGTAAAACCT
CTACAAATGT
GGTATGGCTG
ATTATGATCA
GTCGACCGAT
GCCCTTGAGA
GCCTTCAACC
CAGTCAGCTC
CTTCCGGTGG
GCGCGGGGCA
TGACTATCGT
CGCCGCACTT
ATGACTGTCT
TCTTTATCAT
GCAACTCGTA
GGACAGGTGC
CGGCAGCGCT
CTGGGTCATT
TTCGGCGAGG
ACCGCTTTCG
CTGGAGCGCG
ACGATGATCG
GCCTGTCGCT
TGCGGTATTC
GGAATCTTGC
ACGCCCTCGC
TCAAGCCTTC
GTCACTGGTC
CCGCCACCAA
ACGTTTCGGC
GAGAAGCAGG
CCATTATCGC
CGGCATGGCG
GCCGACGCGC
TGGGCTACGT
CTTGCTGGCG
TTCGCGACGC
GAGGCTGGAT
GGCCTTCCCC
ATTATGATTC
TTCTCGCTTC
CGGCGGCATC
GGGATGCCCG
CGTTGCAGGC
CATGCTGTCC
AGGCAGGTAG
ATGACGACCA
TCAGGGACAG
CTTCAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGGACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTGC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAC
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTGACGTCT
AAGAAACCAT
TATTATCATG
ACATTAACCT
ATAAAAATAG
GCGTATCACG
AGGCCCTTTC
GTCTTCAAGA
ATTAATTCTA
TATTCTAAAA
ACACAAATGA
TACTTCTAAA
AAAAAATCAT
GAATGGCATC
AACTCTGAAT
CAAATCTTTG
CAGATGCACC
TACTTCTCAT
TTCCACTGTC
ACAT
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