名称: | JME4390 |
---|---|
别称: | Plasmid#129656 |
基因长: | |
质粒宿主: | 酵母菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR,Cas9 |
片段物种: | |
原核抗性: | Amp |
筛选标记: | Leu2 |
荧光标记: | 红色 |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TCTTCCTGCA
GTCCGGCAAA
AAAGGGCAAG
GTGTCACCAC
CCTGCCCTTT
TTCTTTAAAA
CCGAAAAGAT
TACTTCGCGT
TATGCAGGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAC
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGCGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTGACGTCT
AAGAAACCAT
TATTATCATG
ACATTAACCT
ATAAAAATAG
GCGTATCACG
AGGCAGAATT
TCAGATAAAA
AAAATCCTTA
GCTTTCGCTA
AGGATGATTT
CTGGAATTCG
AAGAGCCTCT
GACCTGACGT
CCCCAGTTGC
AAAAGTTGAC
ACAACTCTAG
ATCTGCTTCC
AAATATAGAA
TCATAACAAG
GGTTAGGGTG
TGATTATATA
ATATTGGTCT
TAATTGATGT
GCTAGGGCTT
TAAAAGTTGG
TTAAAATAAC
GCTCTAATGC
CTTTTTAATA
TATTGTCTTT
TTCAAAATCT
CAAATCGGAC
ACTTCTTCGT
GTATGAGACT
CCATTTTTTG
GCTCCGTCAC
GTGATATGTA
TTATCAGCTA
TAGTGGTGTA
AACAAAGTTT
TTTACTAGCT
GTAATGGCAT
TTTGTCGGAG
TGGTAAATCG
CCTTCTTGTT
GTGCGTTCGA
GTTCTGGACT
CTGCACTGGG
CTACTTTGAA
AAATACCTCT
AATGCGCCGA
TGGTTTAGTG
GTAAAATCCA
TCGTTGCCAT
CGATGGGCCC
CCGGTTCGAG
AGACGGGGCC
GGCCATCTGG
GCCCTAGAGC
AATACGCAAA
CCGCCTCTCC
CCGCGCGTTG
GCCGATTCAT
TAATGCAGCT
GGCACGACAG
GTTTCCCGAC
TGGAAAGCGG
GCAGTGAGCG
CAACGCAATT
AATGTGAGTT
AGCTCACTCA
TTAGGCACCC
CAGGCTTTAC
ACTTTATGCT
TCCGGCTCGT
ATGTTGTGTG
GAATTGTGAG
CGGATAACAA
TTTCACACAT
ACTAGAGAAA
GAGGAGAAAT
ACTAGATGGC
TTCCTCCGAA
GACGTTATCA
AAGAGTTCAT
GCGTTTCAAA
GTTCGTATGG
AAGGTTCCGT
TAACGGTCAC
GAGTTCGAAA
TCGAAGGTGA
AGGTGAAGGT
CGTCCGTACG
AAGGTACCCA
GACCGCTAAA
CTGAAAGTTA
CCAAAGGTGG
TCCGCTGCCG
TTCGCTTGGG
ACATCCTGTC
CCCGCAGTTC
CAGTACGGTT
CCAAAGCTTA
CGTTAAACAC
CCGGCTGACA
TCCCGGACTA
CCTGAAACTG
TCCTTCCCGG
AAGGTTTCAA
ATGGGAACGT
GTTATGAACT
TCGAAGACGG
TGGTGTTGTT
ACCGTTACCC
AGGACTCCTC
CCTGCAAGAC
GGTGAGTTCA
TCTACAAAGT
TAAACTGCGT
GGTACCAACT
TCCCGTCCGA
CGGTCCGGTT
ATGCAGAAAA
AAACCATGGG
TTGGGAAGCT
TCCACCGAAC
GTATGTACCC
GGAAGACGGT
GCTCTGAAAG
GTGAAATCAA
AATGCGTCTG
AAACTGAAAG
ACGGTGGTCA
CTACGACGCT
GAAGTTAAAA
CCACCTACAT
GGCTAAAAAA
CCGGTTCAGC
TGCCGGGTGC
TTACAAAACC
GACATCAAAC
TGGACATCAC
CTCCCACAAC
GAAGACTACA
CCATCGTTGA
ACAGTACGAA
CGTGCTGAAG
GTCGTCACTC
CACCGGTGCT
TAATAACGCT
GATAGTGCTA
GTGTAGATCG
CTACTAGAGC
CAGGCATCAA
ATAAAACGAA
AGGCTCAGTC
GAAAGACTGG
GCCTTTCGTT
TTATCTGTTG
TTTGTCGGTG
AACGCTCTCT
ACTAGAGTCA
CACTGGCTCA
CCTTCGGGTG
GGCCTTTCTG
CGTTTATATA
GGCCATCTGG
GCCGGGGGCG
TCTCTGAGCT
AGAAATAGCA
AGTTAAAATA
AGGCTAGTCC
GTTATCAACT
TGAAAAAGTG
GCACCGAGTC
GGTGCTTTTT
TTTACGTCTA
AGAAACCATT
ATTATCATGA
CATTAACCTA
TAAAAATAGG
CGTATCACGA
GGCCCAGATC
CTCTAGAGTC
GAAGCGGCCG
CTATGTCTGA
TAAAAGGATG
TAACATAGGC
AAGCTGCTCG
TGAGTGTTGA
GTACGAACCT
TAGATCCAAA
TCACCCGCAC
CCACGGATAT
ACTTGCTTGA
ATATACAGTA
GTATGCGGCC
GCTAGGTATT
ACCCTGTTAT
CCCTACATAA
CTTCGTATAG
CATACATTAT
ACGAAGTTAT
TCTGAATTCC
GCCTGAGTCA
TCATTTATTT
ACCAGTTGGC
CACAAACCCT
TGACGATCTC
GTATGTCCCC
TCCGACATAC
TCCCGGCCGG
CTGGGTACGT
TCGATAGCGC
TATCGGCATC
GACAAGGTTT
GGGTCCCTAG
CCGATACCGC
ACTACCTGAG
TCACAATCTT
CGGAGGTTTA
GTCTTCCACA
TAGCACGGGC
AAAAGTGCGT
ATATATACAA
GAGCGTTTGC
CAGCCACAGA
TTTTCACTCC
ACACACCACA
TCACACATAC
AACCACACAC
ATCCACAATG
GAACCCGAAA
CTAAGAAGAC
CAAGACTGAC
TCCAAGAAGA
TTGTTCTTCT
CGGCGGCGAC
TTCTGTGGCC
CCGAGGTGAT
TGCCGAGGCC
GTCAAGGTGC
TCAAGTCTGT
TGCTGAGGCC
TCCGGCACCG
AGTTTGTGTT
CGAGGACCGA
CTCATTGGAG
GAGCTGCCAT
TGAGAAGGAG
GGCGAGCCCA
TCACCGACGC
TACTCTCGAC
ATCTGCCGAA
AGGCTGACTC
TATTATGCTC
GGTGCTGTCG
GAGGCGCTGC
CAACACCGTA
TGGACCACTC
CCGACGGACG
AACCGACGTG
CGACCCGAGC
AGGGCCTCCT
CAAGCTGCGA
AAGGACCTGA
ACCTGTACGC
CAACCTGCGA
CCCTGCCAGC
TGCTGTCGCC
CAAGCTCGCC
GATCTCTCCC
CCATCCGAAA
CGTTGAGGGC
ACCGACTTCA
TCATTGTCCG
AGAGCTCGTC
GGAGGTATCT
ACTTTGGAGA
GCGAAAGGAG
GATGACGGAT
CTGGCGTCGC
TTCCGACACC
GAGACTTACT
CCGTTCCTGA
GGTTGAGCGA
ATTGCCCGAA
TGGCCGCCTT
CCTGGCCCTT
CAGCACAACC
CCCCTCTTCC
CGTGTGGTCC
CTTGACAAGG
CCAACGTGCT
GGCCTCCTCT
CGACTTTGGC
GAAAGACTGT
CACCCGAGTC
CTCAAGGACG
AGTTCCCCCA
GCTGGAGCTC
AACCACCAGC
TGATCGACTC
GGCCGCCATG
ATCCTCATCA
AGCAGCCCTC
CAAGATGAAT
GGTATCATCA
TCACCACCAA
CATGTTTGGC
GATATCATCT
CCGACGAGGC
CTCCGTCATC
CCCGGTTCTC
TGGGTCTGCT
GCCCTCCGCC
TCTCTGGCTT
CTCTGCCCGA
CACCAACGAG
GCGTTCGGTC
TGTACGAGCC
CTGTCACGGA
TCTGCCCCCG
ATCTCGGCAA
GCAGAAGGTC
AACCCCATTG
CCACCATTCT
GTCTGCCGCC
ATGATGCTCA
AGTTCTCTCT
TAACATGAAG
CCCGCCGGTG
ACGCTGTTGA
GGCTGCCGTC
AAGGAGTCCG
TCGAGGCTGG
TATCACTACC
GCCGATATCG
GAGGCTCTTC
CTCCACCTCC
GAGGTCGGAG
ACTTTGTTGC
CAACAAGGTC
AAGGAGCTGC
TCAAGAAGGA
GTAAGTCGTT
TCTACGACGC
ATTGATGGAA
GGAGCAAACT
GACGCGCCTG
CGGGTTGGTC
TACCGGCAGC
AGCCCTGCTA
ATGAAATGAT
GATTTATAAT
TTACCGGTGT
AGCAACCTTG
ACTAGAAGAA
GCAGATTGGG
TGTGTTTGTA
GTGGAGGACA
GTGGTACGTT
TTGGAAACAG
TCTTCTTGAA
AGTGTCTTGT
CTACAGTATA
TTCACTCATA
ACCTCAATAG
CCAAGGGTGT
AGTCGGTTTA
TTAAAGGAAG
GGAGTTGTGG
CTGATGTGGA
TAGATATCTT
TAAAGCTGGC
GACTGCACCC
AACGAGTGTG
GTGGTAGCTT
GTTACTGTAT
ATTCGAATTC
GTATAACTTC
GTATAGCAGG
AGTTATCCGA
AGCGATAATT
ACCCTGTTAT
CCCTAACGGG
GCCCTTTCGT
CACGCGCGTT
TCGGTGATGA
CGGTGAAAAC
CTCTGACACA
TGCAGCTCCC
GGTGACGGTC
ACAGCTTGTC
TGTAAGCGGA
TGCCGGGAGC
AGACAAGCCC
GTCAGGGCGC
GTCAGCGGGT
GTTGGCGGGT
GTCGGGGCTG
GCTTAACTAT
GCGGCATCAG
AGCAGATTGT
ACTGAGAGTG
CACCATATGC
GGTGTGAAAT
ACCGCACAGA
TGCGTAAGGA
GAAAATACCG
CATCAGGCGC
CATTCGCCAT
TCAGGCTGCG
CAACTGTTGG
GAAGGGCGAT
CGGTGCGGGC
CTCTTCGCTA
TTACGCCAGC
TGGCGAAAGG
GGGATGTGCT
GCAAGGCGAT
TAAGTTGGGT
AACGCCAGGG
TTTTCCCAGT
CACGACGTTG
TAAAACGACG
GCCAGTGAAT
TCGAGCTCGG
TACCCGCTTT
TCGTAGATAA
TGGAATACAA
ATGGATATCC
AGAGTATACA
CATGGATAGT
ATACACTGAC
ACGACAATTC
TGTATCTCTT
TATGTTAACT
ACTGTGAGGC
GTTAAATAGA
GCTTGATATA
TAAAATGTTA
CATTTCACAG
TCTGAACTTT
TGCAGATTAC
CTAATTTGGT
AAGATATTAA
TTATGAACTG
AAAGTTGATG
GCATCCCTAA
ATTTGATGAA
AGGGGGAACC
CCCGGGTTCG
AAGGTACCAA
GGAAGCATGC
GGTACCCGAA
TTCGCTTCGA
TACGCGTATC
GATGATACGC
GTCCATGGTG
AGGTGTCTCA
CAAGTGCCGT
GCAGTCCCGC
CCCCACTTGC
TTCTCTTTGT
GTGTAGTGTA
CGTACATTAT
CGAGAGGGTT
GTTCCCGCCC
ACCTCGATCC
GGCATGCTGA
GGTGTCTCAC
AAGTGCCGTG
CAGTCCCGCC
CCCACTTGCT
TCTCTTTGTG
TGTAGTGTAC
GTACATTATC
GAGAGGGTTG
TTCCCGCCCA
CCTCGATCCG
GCATGCTGAG
GTGTCTCACA
AGTGCCGTGC
AGTCCCGCCC
CCACTTGCTT
CTCTTTGTGT
GTAGTGTACG
TACATTATCG
AGAGGGTTGT
TCCCGCCCAC
CTCGATCCGG
CATGCTGAGG
TGTCTCACAA
GTGCCGTGCA
GTCCCGCCCC
CACTTGCTTC
TCTTTGTGTG
TAGTGTACGT
ACATTATCGA
GAGGGTTGTT
CCCGCCCACC
TCGATCCGGC
ATGCTGAGGT
GTCTCACAAG
TGCCGTGCAG
TCCCGCCCCC
ACTTGCTTCT
CTTTGTGTGT
AGTGTACGTA
CATTATCGAG
AGGGTTGTTC
CCGCCCACCT
CGATCCGGCA
TGCTGAGGTG
TCTCACAAGT
GCCGTGCAGT
CCCGCCCCCA
CTTGCTTCTC
TTTGTGTGTA
GTGTACGTAC
ATTATCGAGA
GGGTTGTTCC
CGCCCACCTC
GATCCGGCAT
GCTGAGGTGT
CTCACAAGTG
CCGTGCAGTC
CCGCCCCCAC
TTGCTTCTCT
TTGTGTGTAG
TGTACGTACA
TTATCGAGAG
GGTTGTTCCC
GCCCACCTCG
ATCCGACATG
TTGAGGTGTC
TCACAAGTGC
CGTGCAGTCC
CGCCCCCACT
TGCTTCTCTT
TGTGTGTAGT
GTACGTACAT
TATCGAGAGG
GTTGTTCCCG
CCCACCTCGA
TCCGGCATGC
ACTGATCACG
GGCAAAAGTG
CTTCGATAGA
GAGGGGGTTG
GCGGCGCATT
TGTGTCCCAA
AAAACAGCCC
CAATTGCCCC
AATTGACCCC
AAATTGACCC
AGTAGCGGAC
CCAACCCCGG
CGAGAGCCCC
CTTCACCCCA
CATATCAAAC
CTCCCCCGGT
TCCCACACTT
GCCGTTAAGG
GCGTAGGGTA
CTGCAGTCTG
GAATCTACGC
TTGTTCAGAC
TTTGTACTAG
TTTCTTTGTC
TGGCCATCCG
GGTAACCCAT
GCCGGACGCA
AAATAGACTA
CTGAAAATTT
TTTTGCTTTG
TGGTTGGGAC
TTTAGCCAAG
GGTATAAAAG
ACCACCGTCC
CCGAATTACC
TTTCCTCTTC
TTTTCTCTCT
CTCCTTGTCA
ACTCACACCC
GAAGGATCCA
CAACCACAAT
GGATAAGAAA
TACTCCATTG
GCCTGGACAT
CGGAACCAAC
TCCGTGGGTT
GGGCCGTGAT
CACCGATGAG
TACAAGGTGC
CCTCTAAGAA
ATTCAAGGTC
CTGGGCAACA
CCGACCGACA
CTCCATCAAG
AAGAACCTGA
TCGGCGCTCT
GCTCTTCGAC
TCTGGCGAGA
CTGCTGAGGC
CACCCGACTG
AAGCGAACCG
CTCGAAGACG
ATACACCCGA
AGAAAGAACC
GAATCTGTTA
CCTGCAGGAG
ATCTTCTCTA
ACGAGATGGC
CAAGGTGGAC
GACTCTTTCT
TCCACCGACT
GGAGGAGTCT
TTCCTGGTGG
AGGAGGACAA
GAAGCACGAG
CGACACCCCA
TCTTCGGCAA
CATCGTGGAC
GAGGTGGCCT
ACCACGAGAA
GTACCCCACC
ATCTACCACC
TGCGAAAGAA
GCTGGTGGAC
TCTACCGACA
AGGCCGACCT
GCGACTGATC
TACCTGGCCC
TGGCCCACAT
GATCAAGTTC
CGAGGCCACT
TCCTGATCGA
GGGCGACCTG
AACCCCGACA
ACTCTGACGT
GGACAAGCTG
TTCATCCAGC
TGGTGCAGAC
CTACAACCAG
CTCTTCGAAG
AGAACCCCAT
TAACGCTTCT
GGCGTGGATG
CTAAGGCCAT
CCTGTCTGCC
CGACTGTCTA
AGTCTCGACG
ACTCGAGAAC
CTGATTGCTC
AGCTCCCCGG
AGAGAAGAAG
AACGGTCTGT
TCGGAAACCT
GATTGCTCTG
TCCCTGGGTC
TGACCCCTAA
CTTCAAGTCC
AACTTCGATC
TGGCTGAGGA
CGCTAAGCTG
CAGCTGTCTA
AGGACACCTA
CGACGATGAC
CTGGATAACC
TGCTCGCCCA
GATTGGCGAC
CAGTACGCCG
ACCTGTTCCT
GGCCGCCAAG
AACCTGTCTG
ACGCCATCCT
GCTGTCTGAC
ATCCTGCGAG
TGAACACCGA
GATCACCAAG
GCCCCCCTGT
CTGCCTCCAT
GATTAAGCGA
TACGATGAGC
ACCACCAGGA
TCTGACCCTC
CTCAAGGCTC
TGGTCCGACA
GCAGCTGCCC
GAGAAGTACA
AGGAGATTTT
CTTCGACCAG
TCTAAGAACG
GCTACGCCGG
CTACATCGAC
GGCGGCGCCT
CTCAGGAGGA
GTTCTACAAG
TTCATTAAGC
CCATCCTGGA
GAAGATGGAC
GGAACCGAGG
AACTGCTCGT
GAAGCTGAAC
CGAGAGGACC
TCCTGCGAAA
GCAGCGAACC
TTCGACAACG
GCTCTATCCC
CCACCAGATC
CACCTGGGCG
AGCTGCACGC
CATCCTGCGA
CGACAGGAGG
ACTTCTACCC
CTTCCTGAAG
GACAACCGAG
AGAAGATCGA
GAAGATCCTG
ACCTTCCGAA
TCCCCTACTA
CGTGGGACCC
CTGGCCCGAG
GAAACTCTCG
ATTCGCTTGG
ATGACCCGAA
AGTCTGAGGA
GACTATTACC
CCCTGGAACT
TCGAGGAGGT
GGTGGATAAG
GGCGCCTCTG
CTCAGTCTTT
CATCGAGCGA
ATGACCAACT
TCGACAAGAA
CCTCCCCAAC
GAGAAGGTCC
TGCCCAAGCA
CTCTCTGCTC
TACGAGTACT
TCACCGTCTA
CAACGAGCTC
ACCAAGGTCA
AGTACGTGAC
CGAGGGAATG
CGAAAGCCCG
CTTTCCTGTC
TGGAGAGCAG
AAGAAGGCTA
TTGTGGATCT
GCTCTTCAAG
ACTAACCGAA
AGGTCACCGT
CAAGCAGCTG
AAGGAGGATT
ACTTCAAGAA
GATTGAGTGT
TTCGATTCTG
TCGAGATCTC
CGGCGTCGAG
GACCGATTCA
ACGCCTCTCT
GGGTACCTAC
CACGACCTGC
TGAAGATTAT
CAAGGACAAG
GATTTCCTGG
ATAACGAGGA
GAACGAGGAT
ATTCTCGAGG
ACATTGTCCT
GACCCTCACC
CTGTTCGAGG
ATCGAGAGAT
GATTGAGGAG
CGACTCAAGA
CCTACGCTCA
CCTGTTCGAC
GACAAGGTGA
TGAAGCAGCT
GAAGCGACGA
CGATACACCG
GCTGGGGCCG
ACTGTCTCGA
AAGCTGATCA
ACGGCATCCG
AGACAAGCAG
TCTGGCAAGA
CCATCCTGGA
CTTCCTGAAG
TCTGACGGCT
TCGCCAACCG
AAACTTCATG
CAGCTGATCC
ACGACGACTC
TCTGACCTTC
AAGGAGGACA
TCCAGAAGGC
CCAGGTGTCT
GGCCAGGGCG
ACTCTCTGCA
CGAGCACATC
GCCAACCTGG
CCGGCTCTCC
CGCCATTAAG
AAAGGTATCC
TGCAGACCGT
CAAGGTGGTC
GATGAGCTCG
TCAAGGTGAT
GGGCCGACAC
AAGCCCGAGA
ACATTGTCAT
TGAGATGGCT
CGAGAGAACC
AGACTACTCA
GAAGGGTCAG
AAAAACTCCC
GAGAGCGAAT
GAAGCGAATT
GAGGAAGGTA
TTAAGGAGCT
GGGCTCCCAG
ATTCTCAAGG
AGCATCCCGT
GGAGAACACT
CAGCTCCAGA
ACGAGAAGCT
GTACCTGTAC
TATCTGCAGA
ACGGTCGAGA
CATGTACGTC
GACCAGGAGC
TGGATATCAA
CCGACTCTCC
GACTACGATG
TGGACCACAT
TGTGCCCCAG
TCCTTCCTGA
AGGACGATTC
TATCGATAAC
AAGGTGCTGA
CCCGATCCGA
CAAGAACCGA
GGCAAGTCTG
ACAACGTGCC
CTCCGAGGAG
GTGGTCAAGA
AGATGAAGAA
CTACTGGCGA
CAGCTGCTGA
ACGCCAAGCT
GATTACCCAG
CGAAAGTTCG
ACAACCTGAC
CAAGGCCGAG
CGAGGCGGCC
TGTCTGAGCT
GGACAAGGCC
GGCTTCATCA
AGCGACAGCT
GGTGGAGACT
CGACAGATCA
CCAAGCACGT
GGCCCAGATC
CTGGACTCTC
GAATGAACAC
CAAGTACGAC
GAGAACGACA
AGCTGATCCG
AGAGGTGAAG
GTGATCACCC
TGAAGTCTAA
GCTGGTGTCT
GACTTCCGAA
AGGACTTCCA
GTTCTACAAG
GTGCGAGAGA
TTAACAACTA
CCACCACGCC
CACGATGCCT
ACCTGAACGC
TGTCGTGGGC
ACCGCCCTCA
TCAAGAAGTA
TCCCAAGCTG
GAGTCCGAGT
TCGTCTACGG
CGACTACAAG
GTCTACGATG
TGCGAAAAAT
GATTGCCAAG
TCCGAGCAGG
AGATTGGCAA
GGCTACCGCC
AAGTACTTCT
TCTACTCCAA
CATTATGAAC
TTCTTCAAGA
CCGAGATTAC
CCTGGCTAAC
GGCGAGATTC
GAAAGCGACC
CCTCATTGAG
ACTAACGGAG
AGACTGGTGA
GATCGTGTGG
GACAAGGGAC
GAGACTTCGC
CACCGTGCGA
AAGGTGCTGT
CTATGCCCCA
GGTGAACATC
GTGAAGAAGA
CCGAGGTGCA
GACCGGAGGT
TTCTCTAAGG
AGTCCATCCT
GCCCAAGCGA
AACTCTGACA
AGCTGATCGC
CCGAAAGAAG
GACTGGGACC
CCAAGAAGTA
CGGAGGTTTC
GACTCTCCCA
CCGTGGCTTA
CTCTGTGCTG
GTGGTGGCCA
AGGTGGAGAA
GGGCAAGTCT
AAGAAGCTGA
AGTCTGTGAA
GGAGCTGCTG
GGCATTACCA
TCATGGAGCG
ATCTTCTTTC
GAGAAGAACC
CCATTGACTT
CCTGGAGGCC
AAGGGATACA
AGGAGGTGAA
GAAAGATCTG
ATTATCAAGC
TCCCCAAGTA
CTCTCTGTTC
GAGCTGGAGA
ACGGACGAAA
GCGAATGCTG
GCCTCTGCCG
GCGAGCTGCA
GAAGGGAAAC
GAGCTGGCCC
TGCCCTCCAA
GTACGTCAAC
TTCCTGTACC
TCGCCTCCCA
TTACGAGAAG
CTGAAGGGCT
CTCCCGAGGA
TAACGAGCAG
AAGCAGCTCT
TCGTGGAGCA
GCATAAGCAC
TACCTGGACG
AGATCATCGA
GCAGATTTCT
GAGTTCTCTA
AGCGAGTGAT
CCTGGCTGAC
GCCAACCTGG
ATAAGGTGCT
GTCTGCTTAC
AACAAGCACC
GAGACAAGCC
CATTCGAGAG
CAGGCTGAGA
ACATCATTCA
CCTGTTCACC
CTGACCAACC
TGGGAGCCCC
CGCTGCCTTC
AAGTACTTCG
ACACCACCAT
CGACCGAAAG
CGATACACCT
CTACCAAGGA
GGTGCTGGAC
GCCACCCTGA
TCCACCAGTC
TATCACCGGC
CTGTACGAGA
CTCGAATCGA
CCTGTCTCAG
CTGGGCGGCG
ACTCTCGAGC
CGACCCCAAG
AAGAAGCGAA
AGGTGTAAGC
TAGCCTCATG
TAATTAGTTA
TGTCACGCTT
ACATTCACGC
CCTCCCCCCA
CATCCGCTCT
AACCGAAAAG
GAAGGAGTTA
GACAACCTGA
AGTCTAGGTC
CCTATTTATT
TTTTTATAGT
TATGTTAGTA
TTAAGAACGT
TATTTATATT
TCAAATTTTT
CTTTTTTTTC
TGTACAGACG
CGTGTACGCA
TGTAACATTA
TACTGAAAAC
CTTGCTTGAG
AAGGTTTTGG
GACGCTCGAA
GGCTTTAATT
TGCAAGCTTG
GCGGTCT