名称: | JME4599 |
---|---|
别称: | Plasmid#129660 |
基因长: | |
质粒宿主: | 酵母菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR,Cas9 |
片段物种: | |
原核抗性: | Amp |
筛选标记: | NAT |
荧光标记: | 红色 |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TCTTCCTGCA
GTCCGGCAAA
AAAGGGCAAG
GTGTCACCAC
CCTGCCCTTT
TTCTTTAAAA
CCGAAAAGAT
TACTTCGCGT
TATGCAGGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAC
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGCGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTGACGTCT
AAGAAACCAT
TATTATCATG
ACATTAACCT
ATAAAAATAG
GCGTATCACG
AGGCAGAATT
TCAGATAAAA
AAAATCCTTA
GCTTTCGCTA
AGGATGATTT
CTGGAATTCG
AAGAGCCTCT
GACCTGACGT
CCCCAGTTGC
AAAAGTTGAC
ACAACTCTAG
ATCTGCTTCC
AAATATAGAA
TCATAACAAG
GGTTAGGGTG
TGATTATATA
ATATTGGTCT
TAATTGATGT
GCTAGGGCTT
TAAAAGTTGG
TTAAAATAAC
GCTCTAATGC
CTTTTTAATA
TATTGTCTTT
TTCAAAATCT
CAAATCGGAC
ACTTCTTCGT
GTATGAGACT
CCATTTTTTG
GCTCCGTCAC
GTGATATGTA
TTATCAGCTA
TAGTGGTGTA
AACAAAGTTT
TTTACTAGCT
GTAATGGCAT
TTTGTCGGAG
TGGTAAATCG
CCTTCTTGTT
GTGCGTTCGA
GTTCTGGACT
CTGCACTGGG
CTACTTTGAA
AAATACCTCT
AATGCGCCGA
TGGTTTAGTG
GTAAAATCCA
TCGTTGCCAT
CGATGGGCCC
CCGGTTCGAG
AGACGGGGCC
GGCCATCTGG
GCCCTAGAGC
AATACGCAAA
CCGCCTCTCC
CCGCGCGTTG
GCCGATTCAT
TAATGCAGCT
GGCACGACAG
GTTTCCCGAC
TGGAAAGCGG
GCAGTGAGCG
CAACGCAATT
AATGTGAGTT
AGCTCACTCA
TTAGGCACCC
CAGGCTTTAC
ACTTTATGCT
TCCGGCTCGT
ATGTTGTGTG
GAATTGTGAG
CGGATAACAA
TTTCACACAT
ACTAGAGAAA
GAGGAGAAAT
ACTAGATGGC
TTCCTCCGAA
GACGTTATCA
AAGAGTTCAT
GCGTTTCAAA
GTTCGTATGG
AAGGTTCCGT
TAACGGTCAC
GAGTTCGAAA
TCGAAGGTGA
AGGTGAAGGT
CGTCCGTACG
AAGGTACCCA
GACCGCTAAA
CTGAAAGTTA
CCAAAGGTGG
TCCGCTGCCG
TTCGCTTGGG
ACATCCTGTC
CCCGCAGTTC
CAGTACGGTT
CCAAAGCTTA
CGTTAAACAC
CCGGCTGACA
TCCCGGACTA
CCTGAAACTG
TCCTTCCCGG
AAGGTTTCAA
ATGGGAACGT
GTTATGAACT
TCGAAGACGG
TGGTGTTGTT
ACCGTTACCC
AGGACTCCTC
CCTGCAAGAC
GGTGAGTTCA
TCTACAAAGT
TAAACTGCGT
GGTACCAACT
TCCCGTCCGA
CGGTCCGGTT
ATGCAGAAAA
AAACCATGGG
TTGGGAAGCT
TCCACCGAAC
GTATGTACCC
GGAAGACGGT
GCTCTGAAAG
GTGAAATCAA
AATGCGTCTG
AAACTGAAAG
ACGGTGGTCA
CTACGACGCT
GAAGTTAAAA
CCACCTACAT
GGCTAAAAAA
CCGGTTCAGC
TGCCGGGTGC
TTACAAAACC
GACATCAAAC
TGGACATCAC
CTCCCACAAC
GAAGACTACA
CCATCGTTGA
ACAGTACGAA
CGTGCTGAAG
GTCGTCACTC
CACCGGTGCT
TAATAACGCT
GATAGTGCTA
GTGTAGATCG
CTACTAGAGC
CAGGCATCAA
ATAAAACGAA
AGGCTCAGTC
GAAAGACTGG
GCCTTTCGTT
TTATCTGTTG
TTTGTCGGTG
AACGCTCTCT
ACTAGAGTCA
CACTGGCTCA
CCTTCGGGTG
GGCCTTTCTG
CGTTTATATA
GGCCATCTGG
GCCGGGGGCG
TCTCTGAGCT
AGAAATAGCA
AGTTAAAATA
AGGCTAGTCC
GTTATCAACT
TGAAAAAGTG
GCACCGAGTC
GGTGCTTTTT
TTTACGTCTA
AGAAACCATT
ATTATCATGA
CATTAACCTA
TAAAAATAGG
CGTATCACGA
GGCCCAGATC
CTCTAGAGTC
GAAGCGGCCG
CTATGTCTGA
TAAAAGGATG
TAACATAGGC
AAGCTGCTCG
TGAGTGTTGA
GTACGAACCT
TAGATCCAAA
TCACCCGCAC
CCACGGATAT
ACTTGCTTGA
ATATACAGTA
GTATGCGGCC
GCTAGGTTAG
GGATAACAGG
GTAATGATAA
CTTCGTATAG
CATACATTAT
ACGAAGTTAT
GGGGTTGGCG
GCGCATTTGT
GTCCCAAAAA
ACAGCCCCAA
TTGCCCCAAT
TGACCCCAAA
TTGACCCAGT
AGCGGACCCA
ACCCCGGCGA
GAGCCCCCTT
CACCCCACAT
ATCAAACCTC
CCCCGGTTCC
CACACTTGCC
GTTAAGGGCG
TAGGGTACTG
CAGTCTGGAA
TCTACGCTTG
TTCAGACTTT
GTACTAGTTT
CTTTGTCTGG
CCATCCGGGT
AACCCATGCC
GGACGCAAAA
TAGACTACTG
AAAATTTTTT
TGCTTTGTGG
TTGGGACTTT
AGCCAAGGGT
ATAAAAGACC
ACCGTCCCCG
AATTACCTTT
CCTCTTCTTT
TCTCTCTCTC
CTTGTCAACT
CACACCCGAA
GCACAATGAC
CACTCTGGAT
GACACCGCTT
ACCGATACCG
AACTTCCGTT
CCTGGCGATG
CCGAGGCTAT
TGAGGCTCTG
GATGGATCTT
TCACCACTGA
CACCGTTTTC
CGAGTGACCG
CTACTGGCGA
CGGCTTCACC
CTGCGAGAGG
TGCCTGTCGA
CCCTCCTCTC
ACCAAGGTTT
TCCCTGACGA
TGAGTCGGAC
GATGAGTCTG
ACGCTGGAGA
GGACGGCGAC
CCTGACTCTC
GAACTTTCGT
GGCTTACGGC
GACGATGGCG
ACCTGGCCGG
CTTTGTGGTC
GTTTCTTACT
CCGGATGGAA
CCGACGACTG
ACCGTGGAGG
ACATCGAGGT
CGCTCCTGAG
CACCGAGGTC
ATGGTGTCGG
ACGAGCTCTG
ATGGGTCTGG
CTACTGAGTT
CGCTCGAGAG
CGAGGTGCTG
GCCACCTGTG
GCTCGAGGTC
ACCAACGTTA
ACGCCCCTGC
TATTCATGCC
TACCGACGAA
TGGGTTTTAC
CCTGTGTGGC
CTCGATACTG
CCCTGTACGA
CGGAACCGCT
TCCGATGGAG
AGCAGGCCCT
CTACATGTCG
ATGCCCTGCC
CTTAAGTGTC
TGTGGTATCT
AAGCTATTTA
TCACTCTTTA
CAACTTCTAC
CTCAACTATC
TACTTTAATA
AATGAATATC
GTTTATTCTC
TATGATTACT
GTATATGCGT
TCCTCTAAGA
CAAATCGAAT
ATAACTTCGT
ATAGCAGGAG
TTATCCGAAG
CGAGTAGGGA
TAACAGGGTA
ATACGGGGCC
CTTTCGTCAC
GCGCGTTTCG
GTGATGACGG
TGAAAACCTC
TGACACATGC
AGCTCCCGGT
GACGGTCACA
GCTTGTCTGT
AAGCGGATGC
CGGGAGCAGA
CAAGCCCGTC
AGGGCGCGTC
AGCGGGTGTT
GGCGGGTGTC
GGGGCTGGCT
TAACTATGCG
GCATCAGAGC
AGATTGTACT
GAGAGTGCAC
CATATGCGGT
GTGAAATACC
GCACAGATGC
GTAAGGAGAA
AATACCGCAT
CAGGCGCCAT
TCGCCATTCA
GGCTGCGCAA
CTGTTGGGAA
GGGCGATCGG
TGCGGGCCTC
TTCGCTATTA
CGCCAGCTGG
CGAAAGGGGG
ATGTGCTGCA
AGGCGATTAA
GTTGGGTAAC
GCCAGGGTTT
TCCCAGTCAC
GACGTTGTAA
AACGACGGCC
AGTGAATTCG
AGCTCGGTAC
CCGCTTTTCG
TAGATAATGG
AATACAAATG
GATATCCAGA
GTATACACAT
GGATAGTATA
CACTGACACG
ACAATTCTGT
ATCTCTTTAT
GTTAACTACT
GTGAGGCGTT
AAATAGAGCT
TGATATATAA
AATGTTACAT
TTCACAGTCT
GAACTTTTGC
AGATTACCTA
ATTTGGTAAG
ATATTAATTA
TGAACTGAAA
GTTGATGGCA
TCCCTAAATT
TGATGAAAGG
GGGAACCCCC
GGGTTCGAAG
GTACCAAGGA
AGCATGCGGT
ACCCGAATTC
GCTTCGATAC
GCGTATCGAT
GATACGCGTC
CATGGTGAGG
TGTCTCACAA
GTGCCGTGCA
GTCCCGCCCC
CACTTGCTTC
TCTTTGTGTG
TAGTGTACGT
ACATTATCGA
GAGGGTTGTT
CCCGCCCACC
TCGATCCGGC
ATGCTGAGGT
GTCTCACAAG
TGCCGTGCAG
TCCCGCCCCC
ACTTGCTTCT
CTTTGTGTGT
AGTGTACGTA
CATTATCGAG
AGGGTTGTTC
CCGCCCACCT
CGATCCGGCA
TGCTGAGGTG
TCTCACAAGT
GCCGTGCAGT
CCCGCCCCCA
CTTGCTTCTC
TTTGTGTGTA
GTGTACGTAC
ATTATCGAGA
GGGTTGTTCC
CGCCCACCTC
GATCCGGCAT
GCTGAGGTGT
CTCACAAGTG
CCGTGCAGTC
CCGCCCCCAC
TTGCTTCTCT
TTGTGTGTAG
TGTACGTACA
TTATCGAGAG
GGTTGTTCCC
GCCCACCTCG
ATCCGGCATG
CTGAGGTGTC
TCACAAGTGC
CGTGCAGTCC
CGCCCCCACT
TGCTTCTCTT
TGTGTGTAGT
GTACGTACAT
TATCGAGAGG
GTTGTTCCCG
CCCACCTCGA
TCCGGCATGC
TGAGGTGTCT
CACAAGTGCC
GTGCAGTCCC
GCCCCCACTT
GCTTCTCTTT
GTGTGTAGTG
TACGTACATT
ATCGAGAGGG
TTGTTCCCGC
CCACCTCGAT
CCGGCATGCT
GAGGTGTCTC
ACAAGTGCCG
TGCAGTCCCG
CCCCCACTTG
CTTCTCTTTG
TGTGTAGTGT
ACGTACATTA
TCGAGAGGGT
TGTTCCCGCC
CACCTCGATC
CGACATGTTG
AGGTGTCTCA
CAAGTGCCGT
GCAGTCCCGC
CCCCACTTGC
TTCTCTTTGT
GTGTAGTGTA
CGTACATTAT
CGAGAGGGTT
GTTCCCGCCC
ACCTCGATCC
GGCATGCACT
GATCACGGGC
AAAAGTGCTT
CGATAGAGAG
GGGGTTGGCG
GCGCATTTGT
GTCCCAAAAA
ACAGCCCCAA
TTGCCCCAAT
TGACCCCAAA
TTGACCCAGT
AGCGGACCCA
ACCCCGGCGA
GAGCCCCCTT
CACCCCACAT
ATCAAACCTC
CCCCGGTTCC
CACACTTGCC
GTTAAGGGCG
TAGGGTACTG
CAGTCTGGAA
TCTACGCTTG
TTCAGACTTT
GTACTAGTTT
CTTTGTCTGG
CCATCCGGGT
AACCCATGCC
GGACGCAAAA
TAGACTACTG
AAAATTTTTT
TGCTTTGTGG
TTGGGACTTT
AGCCAAGGGT
ATAAAAGACC
ACCGTCCCCG
AATTACCTTT
CCTCTTCTTT
TCTCTCTCTC
CTTGTCAACT
CACACCCGAA
GGATCCACAA
CCACAATGGA
TAAGAAATAC
TCCATTGGCC
TGGACATCGG
AACCAACTCC
GTGGGTTGGG
CCGTGATCAC
CGATGAGTAC
AAGGTGCCCT
CTAAGAAATT
CAAGGTCCTG
GGCAACACCG
ACCGACACTC
CATCAAGAAG
AACCTGATCG
GCGCTCTGCT
CTTCGACTCT
GGCGAGACTG
CTGAGGCCAC
CCGACTGAAG
CGAACCGCTC
GAAGACGATA
CACCCGAAGA
AAGAACCGAA
TCTGTTACCT
GCAGGAGATC
TTCTCTAACG
AGATGGCCAA
GGTGGACGAC
TCTTTCTTCC
ACCGACTGGA
GGAGTCTTTC
CTGGTGGAGG
AGGACAAGAA
GCACGAGCGA
CACCCCATCT
TCGGCAACAT
CGTGGACGAG
GTGGCCTACC
ACGAGAAGTA
CCCCACCATC
TACCACCTGC
GAAAGAAGCT
GGTGGACTCT
ACCGACAAGG
CCGACCTGCG
ACTGATCTAC
CTGGCCCTGG
CCCACATGAT
CAAGTTCCGA
GGCCACTTCC
TGATCGAGGG
CGACCTGAAC
CCCGACAACT
CTGACGTGGA
CAAGCTGTTC
ATCCAGCTGG
TGCAGACCTA
CAACCAGCTC
TTCGAAGAGA
ACCCCATTAA
CGCTTCTGGC
GTGGATGCTA
AGGCCATCCT
GTCTGCCCGA
CTGTCTAAGT
CTCGACGACT
CGAGAACCTG
ATTGCTCAGC
TCCCCGGAGA
GAAGAAGAAC
GGTCTGTTCG
GAAACCTGAT
TGCTCTGTCC
CTGGGTCTGA
CCCCTAACTT
CAAGTCCAAC
TTCGATCTGG
CTGAGGACGC
TAAGCTGCAG
CTGTCTAAGG
ACACCTACGA
CGATGACCTG
GATAACCTGC
TCGCCCAGAT
TGGCGACCAG
TACGCCGACC
TGTTCCTGGC
CGCCAAGAAC
CTGTCTGACG
CCATCCTGCT
GTCTGACATC
CTGCGAGTGA
ACACCGAGAT
CACCAAGGCC
CCCCTGTCTG
CCTCCATGAT
TAAGCGATAC
GATGAGCACC
ACCAGGATCT
GACCCTCCTC
AAGGCTCTGG
TCCGACAGCA
GCTGCCCGAG
AAGTACAAGG
AGATTTTCTT
CGACCAGTCT
AAGAACGGCT
ACGCCGGCTA
CATCGACGGC
GGCGCCTCTC
AGGAGGAGTT
CTACAAGTTC
ATTAAGCCCA
TCCTGGAGAA
GATGGACGGA
ACCGAGGAAC
TGCTCGTGAA
GCTGAACCGA
GAGGACCTCC
TGCGAAAGCA
GCGAACCTTC
GACAACGGCT
CTATCCCCCA
CCAGATCCAC
CTGGGCGAGC
TGCACGCCAT
CCTGCGACGA
CAGGAGGACT
TCTACCCCTT
CCTGAAGGAC
AACCGAGAGA
AGATCGAGAA
GATCCTGACC
TTCCGAATCC
CCTACTACGT
GGGACCCCTG
GCCCGAGGAA
ACTCTCGATT
CGCTTGGATG
ACCCGAAAGT
CTGAGGAGAC
TATTACCCCC
TGGAACTTCG
AGGAGGTGGT
GGATAAGGGC
GCCTCTGCTC
AGTCTTTCAT
CGAGCGAATG
ACCAACTTCG
ACAAGAACCT
CCCCAACGAG
AAGGTCCTGC
CCAAGCACTC
TCTGCTCTAC
GAGTACTTCA
CCGTCTACAA
CGAGCTCACC
AAGGTCAAGT
ACGTGACCGA
GGGAATGCGA
AAGCCCGCTT
TCCTGTCTGG
AGAGCAGAAG
AAGGCTATTG
TGGATCTGCT
CTTCAAGACT
AACCGAAAGG
TCACCGTCAA
GCAGCTGAAG
GAGGATTACT
TCAAGAAGAT
TGAGTGTTTC
GATTCTGTCG
AGATCTCCGG
CGTCGAGGAC
CGATTCAACG
CCTCTCTGGG
TACCTACCAC
GACCTGCTGA
AGATTATCAA
GGACAAGGAT
TTCCTGGATA
ACGAGGAGAA
CGAGGATATT
CTCGAGGACA
TTGTCCTGAC
CCTCACCCTG
TTCGAGGATC
GAGAGATGAT
TGAGGAGCGA
CTCAAGACCT
ACGCTCACCT
GTTCGACGAC
AAGGTGATGA
AGCAGCTGAA
GCGACGACGA
TACACCGGCT
GGGGCCGACT
GTCTCGAAAG
CTGATCAACG
GCATCCGAGA
CAAGCAGTCT
GGCAAGACCA
TCCTGGACTT
CCTGAAGTCT
GACGGCTTCG
CCAACCGAAA
CTTCATGCAG
CTGATCCACG
ACGACTCTCT
GACCTTCAAG
GAGGACATCC
AGAAGGCCCA
GGTGTCTGGC
CAGGGCGACT
CTCTGCACGA
GCACATCGCC
AACCTGGCCG
GCTCTCCCGC
CATTAAGAAA
GGTATCCTGC
AGACCGTCAA
GGTGGTCGAT
GAGCTCGTCA
AGGTGATGGG
CCGACACAAG
CCCGAGAACA
TTGTCATTGA
GATGGCTCGA
GAGAACCAGA
CTACTCAGAA
GGGTCAGAAA
AACTCCCGAG
AGCGAATGAA
GCGAATTGAG
GAAGGTATTA
AGGAGCTGGG
CTCCCAGATT
CTCAAGGAGC
ATCCCGTGGA
GAACACTCAG
CTCCAGAACG
AGAAGCTGTA
CCTGTACTAT
CTGCAGAACG
GTCGAGACAT
GTACGTCGAC
CAGGAGCTGG
ATATCAACCG
ACTCTCCGAC
TACGATGTGG
ACCACATTGT
GCCCCAGTCC
TTCCTGAAGG
ACGATTCTAT
CGATAACAAG
GTGCTGACCC
GATCCGACAA
GAACCGAGGC
AAGTCTGACA
ACGTGCCCTC
CGAGGAGGTG
GTCAAGAAGA
TGAAGAACTA
CTGGCGACAG
CTGCTGAACG
CCAAGCTGAT
TACCCAGCGA
AAGTTCGACA
ACCTGACCAA
GGCCGAGCGA
GGCGGCCTGT
CTGAGCTGGA
CAAGGCCGGC
TTCATCAAGC
GACAGCTGGT
GGAGACTCGA
CAGATCACCA
AGCACGTGGC
CCAGATCCTG
GACTCTCGAA
TGAACACCAA
GTACGACGAG
AACGACAAGC
TGATCCGAGA
GGTGAAGGTG
ATCACCCTGA
AGTCTAAGCT
GGTGTCTGAC
TTCCGAAAGG
ACTTCCAGTT
CTACAAGGTG
CGAGAGATTA
ACAACTACCA
CCACGCCCAC
GATGCCTACC
TGAACGCTGT
CGTGGGCACC
GCCCTCATCA
AGAAGTATCC
CAAGCTGGAG
TCCGAGTTCG
TCTACGGCGA
CTACAAGGTC
TACGATGTGC
GAAAAATGAT
TGCCAAGTCC
GAGCAGGAGA
TTGGCAAGGC
TACCGCCAAG
TACTTCTTCT
ACTCCAACAT
TATGAACTTC
TTCAAGACCG
AGATTACCCT
GGCTAACGGC
GAGATTCGAA
AGCGACCCCT
CATTGAGACT
AACGGAGAGA
CTGGTGAGAT
CGTGTGGGAC
AAGGGACGAG
ACTTCGCCAC
CGTGCGAAAG
GTGCTGTCTA
TGCCCCAGGT
GAACATCGTG
AAGAAGACCG
AGGTGCAGAC
CGGAGGTTTC
TCTAAGGAGT
CCATCCTGCC
CAAGCGAAAC
TCTGACAAGC
TGATCGCCCG
AAAGAAGGAC
TGGGACCCCA
AGAAGTACGG
AGGTTTCGAC
TCTCCCACCG
TGGCTTACTC
TGTGCTGGTG
GTGGCCAAGG
TGGAGAAGGG
CAAGTCTAAG
AAGCTGAAGT
CTGTGAAGGA
GCTGCTGGGC
ATTACCATCA
TGGAGCGATC
TTCTTTCGAG
AAGAACCCCA
TTGACTTCCT
GGAGGCCAAG
GGATACAAGG
AGGTGAAGAA
AGATCTGATT
ATCAAGCTCC
CCAAGTACTC
TCTGTTCGAG
CTGGAGAACG
GACGAAAGCG
AATGCTGGCC
TCTGCCGGCG
AGCTGCAGAA
GGGAAACGAG
CTGGCCCTGC
CCTCCAAGTA
CGTCAACTTC
CTGTACCTCG
CCTCCCATTA
CGAGAAGCTG
AAGGGCTCTC
CCGAGGATAA
CGAGCAGAAG
CAGCTCTTCG
TGGAGCAGCA
TAAGCACTAC
CTGGACGAGA
TCATCGAGCA
GATTTCTGAG
TTCTCTAAGC
GAGTGATCCT
GGCTGACGCC
AACCTGGATA
AGGTGCTGTC
TGCTTACAAC
AAGCACCGAG
ACAAGCCCAT
TCGAGAGCAG
GCTGAGAACA
TCATTCACCT
GTTCACCCTG
ACCAACCTGG
GAGCCCCCGC
TGCCTTCAAG
TACTTCGACA
CCACCATCGA
CCGAAAGCGA
TACACCTCTA
CCAAGGAGGT
GCTGGACGCC
ACCCTGATCC
ACCAGTCTAT
CACCGGCCTG
TACGAGACTC
GAATCGACCT
GTCTCAGCTG
GGCGGCGACT
CTCGAGCCGA
CCCCAAGAAG
AAGCGAAAGG
TGTAAGCTAG
CCTCATGTAA
TTAGTTATGT
CACGCTTACA
TTCACGCCCT
CCCCCCACAT
CCGCTCTAAC
CGAAAAGGAA
GGAGTTAGAC
AACCTGAAGT
CTAGGTCCCT
ATTTATTTTT
TTATAGTTAT
GTTAGTATTA
AGAACGTTAT
TTATATTTCA
AATTTTTCTT
TTTTTTCTGT
ACAGACGCGT
GTACGCATGT
AACATTATAC
TGAAAACCTT
GCTTGAGAAG
GTTTTGGGAC
GCTCGAAGGC
TTTAATTTGC
AAGCTTGGCG
GTCT