名称: | pX458-HSP70-Fluc-sgRNA2-EnCMV-Cas9-T2A-EGFP |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 绿色 |
启动子: | HSP70 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CACTCTGGCC
TCTGATTGGT
CCAAGGAAGG
CTGGGGGGCA
GGACGGGAGG
CGAAAACCCT
GGAATATTCC
CGACCTGGCA
GCCTCATCGA
GCTCGGTGAT
TGGCTCAGAA
GGGAAAAGGC
GGGTCTCCGT
GACGACTTAT
AAAAGCCCAG
GGGCAAGCGG
TCCGGATAAC
GGCTAGCCTG
AGGAGCTGCT
GCGACAGTCC
ACTACCTTTT
TCGAGAGTGA
CTCCCGTTGT
CCCAAGGCTT
CCCAGAGCGA
ACCTGTGCGG
CTGCAGGCAC
CGGCGCGTCG
AGTTTCCGGC
GTCCGGAAGG
ACCGAGCTCT
TCTCGCGGAT
CCAGTGTTCC
GTTTCCAGAG
AAACACCGAC
ACTGGGTGTG
AACCAGCGGT
TTTAGAGCTA
GAAATAGCAA
GTTAAAATAA
GGCTAGTCCG
TTATCAACTT
GAAAAAGTGG
CACCGAGTCG
GTGCTTTTTT
GTTTTAGAGC
TAGAAATAGC
AAGTTAAAAT
AAGGCTAGTC
CGTTTTTAGC
GCGTGCGCCA
ATTCTGCAGA
CAAATGGCTC
TAGAGGTACC
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
GTAACGCCAA
TAGGGACTTT
CCATTGACGT
CAATGGGTGG
AGTATTTACG
GTAAACTGCC
CACTTGGCAG
TACATCAAGT
GTATCATATG
CCAAGTACGC
CCCCTATTGA
CGTCAATGAC
GGTAAATGGC
CCGCCTGGCA
TTGTGCCCAG
TACATGACCT
TATGGGACTT
TCCTACTTGG
CAGTACATCT
ACGTATTAGT
CATCGCTATT
ACCATGGTCG
AGGTGAGCCC
CACGTTCTGC
TTCACTCTCC
CCATCTCCCC
CCCCTCCCCA
CCCCCAATTT
TGTATTTATT
TATTTTTTAA
TTATTTTGTG
CAGCGATGGG
GGCGGGGGGG
GGGGGGGGGC
GCGCGCCAGG
CGGGGCGGGG
CGGGGCGAGG
GGCGGGGCGG
GGCGAGGCGG
AGAGGTGCGG
CGGCAGCCAA
TCAGAGCGGC
GCGCTCCGAA
AGTTTCCTTT
TATGGCGAGG
CGGCGGCGGC
GGCGGCCCTA
TAAAAAGCGA
AGCGCGCGGC
GGGCGGGAGT
CGCTGCGACG
CTGCCTTCGC
CCCGTGCCCC
GCTCCGCCGC
CGCCTCGCGC
CGCCCGCCCC
GGCTCTGACT
GACCGCGTTA
CTCCCACAGG
TGAGCGGGCG
GGACGGCCCT
TCTCCTCCGG
GCTGTAATTA
GCTGAGCAAG
AGGTAAGGGT
TTAAGGGATG
GTTGGTTGGT
GGGGTATTAA
TGTTTAATTA
CCTGGAGCAC
CTGCCTGAAA
TCACTTTTTT
TCAGGTTGGA
CCGGTGCCAC
CATGGACTAT
AAGGACCACG
ACGGAGACTA
CAAGGATCAT
GATATTGATT
ACAAAGACGA
TGACGATAAG
ATGGCCCCAA
AGAAGAAGCG
GAAGGTCGGT
ATCCACGGAG
TCCCAGCAGC
CGACAAGAAG
TACAGCATCG
GCCTGGACAT
CGGCACCAAC
TCTGTGGGCT
GGGCCGTGAT
CACCGACGAG
TACAAGGTGC
CCAGCAAGAA
ATTCAAGGTG
CTGGGCAACA
CCGACCGGCA
CAGCATCAAG
AAGAACCTGA
TCGGAGCCCT
GCTGTTCGAC
AGCGGCGAAA
CAGCCGAGGC
CACCCGGCTG
AAGAGAACCG
CCAGAAGAAG
ATACACCAGA
CGGAAGAACC
GGATCTGCTA
TCTGCAAGAG
ATCTTCAGCA
ACGAGATGGC
CAAGGTGGAC
GACAGCTTCT
TCCACAGACT
GGAAGAGTCC
TTCCTGGTGG
AAGAGGATAA
GAAGCACGAG
CGGCACCCCA
TCTTCGGCAA
CATCGTGGAC
GAGGTGGCCT
ACCACGAGAA
GTACCCCACC
ATCTACCACC
TGAGAAAGAA
ACTGGTGGAC
AGCACCGACA
AGGCCGACCT
GCGGCTGATC
TATCTGGCCC
TGGCCCACAT
GATCAAGTTC
CGGGGCCACT
TCCTGATCGA
GGGCGACCTG
AACCCCGACA
ACAGCGACGT
GGACAAGCTG
TTCATCCAGC
TGGTGCAGAC
CTACAACCAG
CTGTTCGAGG
AAAACCCCAT
CAACGCCAGC
GGCGTGGACG
CCAAGGCCAT
CCTGTCTGCC
AGACTGAGCA
AGAGCAGACG
GCTGGAAAAT
CTGATCGCCC
AGCTGCCCGG
CGAGAAGAAG
AATGGCCTGT
TCGGAAACCT
GATTGCCCTG
AGCCTGGGCC
TGACCCCCAA
CTTCAAGAGC
AACTTCGACC
TGGCCGAGGA
TGCCAAACTG
CAGCTGAGCA
AGGACACCTA
CGACGACGAC
CTGGACAACC
TGCTGGCCCA
GATCGGCGAC
CAGTACGCCG
ACCTGTTTCT
GGCCGCCAAG
AACCTGTCCG
ACGCCATCCT
GCTGAGCGAC
ATCCTGAGAG
TGAACACCGA
GATCACCAAG
GCCCCCCTGA
GCGCCTCTAT
GATCAAGAGA
TACGACGAGC
ACCACCAGGA
CCTGACCCTG
CTGAAAGCTC
TCGTGCGGCA
GCAGCTGCCT
GAGAAGTACA
AAGAGATTTT
CTTCGACCAG
AGCAAGAACG
GCTACGCCGG
CTACATTGAC
GGCGGAGCCA
GCCAGGAAGA
GTTCTACAAG
TTCATCAAGC
CCATCCTGGA
AAAGATGGAC
GGCACCGAGG
AACTGCTCGT
GAAGCTGAAC
AGAGAGGACC
TGCTGCGGAA
GCAGCGGACC
TTCGACAACG
GCAGCATCCC
CCACCAGATC
CACCTGGGAG
AGCTGCACGC
CATTCTGCGG
CGGCAGGAAG
ATTTTTACCC
ATTCCTGAAG
GACAACCGGG
AAAAGATCGA
GAAGATCCTG
ACCTTCCGCA
TCCCCTACTA
CGTGGGCCCT
CTGGCCAGGG
GAAACAGCAG
ATTCGCCTGG
ATGACCAGAA
AGAGCGAGGA
AACCATCACC
CCCTGGAACT
TCGAGGAAGT
GGTGGACAAG
GGCGCTTCCG
CCCAGAGCTT
CATCGAGCGG
ATGACCAACT
TCGATAAGAA
CCTGCCCAAC
GAGAAGGTGC
TGCCCAAGCA
CAGCCTGCTG
TACGAGTACT
TCACCGTGTA
TAACGAGCTG
ACCAAAGTGA
AATACGTGAC
CGAGGGAATG
AGAAAGCCCG
CCTTCCTGAG
CGGCGAGCAG
AAAAAGGCCA
TCGTGGACCT
GCTGTTCAAG
ACCAACCGGA
AAGTGACCGT
GAAGCAGCTG
AAAGAGGACT
ACTTCAAGAA
AATCGAGTGC
TTCGACTCCG
TGGAAATCTC
CGGCGTGGAA
GATCGGTTCA
ACGCCTCCCT
GGGCACATAC
CACGATCTGC
TGAAAATTAT
CAAGGACAAG
GACTTCCTGG
ACAATGAGGA
AAACGAGGAC
ATTCTGGAAG
ATATCGTGCT
GACCCTGACA
CTGTTTGAGG
ACAGAGAGAT
GATCGAGGAA
CGGCTGAAAA
CCTATGCCCA
CCTGTTCGAC
GACAAAGTGA
TGAAGCAGCT
GAAGCGGCGG
AGATACACCG
GCTGGGGCAG
GCTGAGCCGG
AAGCTGATCA
ACGGCATCCG
GGACAAGCAG
TCCGGCAAGA
CAATCCTGGA
TTTCCTGAAG
TCCGACGGCT
TCGCCAACAG
AAACTTCATG
CAGCTGATCC
ACGACGACAG
CCTGACCTTT
AAAGAGGACA
TCCAGAAAGC
CCAGGTGTCC
GGCCAGGGCG
ATAGCCTGCA
CGAGCACATT
GCCAATCTGG
CCGGCAGCCC
CGCCATTAAG
AAGGGCATCC
TGCAGACAGT
GAAGGTGGTG
GACGAGCTCG
TGAAAGTGAT
GGGCCGGCAC
AAGCCCGAGA
ACATCGTGAT
CGAAATGGCC
AGAGAGAACC
AGACCACCCA
GAAGGGACAG
AAGAACAGCC
GCGAGAGAAT
GAAGCGGATC
GAAGAGGGCA
TCAAAGAGCT
GGGCAGCCAG
ATCCTGAAAG
AACACCCCGT
GGAAAACACC
CAGCTGCAGA
ACGAGAAGCT
GTACCTGTAC
TACCTGCAGA
ATGGGCGGGA
TATGTACGTG
GACCAGGAAC
TGGACATCAA
CCGGCTGTCC
GACTACGATG
TGGACCATAT
CGTGCCTCAG
AGCTTTCTGA
AGGACGACTC
CATCGACAAC
AAGGTGCTGA
CCAGAAGCGA
CAAGAACCGG
GGCAAGAGCG
ACAACGTGCC
CTCCGAAGAG
GTCGTGAAGA
AGATGAAGAA
CTACTGGCGG
CAGCTGCTGA
ACGCCAAGCT
GATTACCCAG
AGAAAGTTCG
ACAATCTGAC
CAAGGCCGAG
AGAGGCGGCC
TGAGCGAACT
GGATAAGGCC
GGCTTCATCA
AGAGACAGCT
GGTGGAAACC
CGGCAGATCA
CAAAGCACGT
GGCACAGATC
CTGGACTCCC
GGATGAACAC
TAAGTACGAC
GAGAATGACA
AGCTGATCCG
GGAAGTGAAA
GTGATCACCC
TGAAGTCCAA
GCTGGTGTCC
GATTTCCGGA
AGGATTTCCA
GTTTTACAAA
GTGCGCGAGA
TCAACAACTA
CCACCACGCC
CACGACGCCT
ACCTGAACGC
CGTCGTGGGA
ACCGCCCTGA
TCAAAAAGTA
CCCTAAGCTG
GAAAGCGAGT
TCGTGTACGG
CGACTACAAG
GTGTACGACG
TGCGGAAGAT
GATCGCCAAG
AGCGAGCAGG
AAATCGGCAA
GGCTACCGCC
AAGTACTTCT
TCTACAGCAA
CATCATGAAC
TTTTTCAAGA
CCGAGATTAC
CCTGGCCAAC
GGCGAGATCC
GGAAGCGGCC
TCTGATCGAG
ACAAACGGCG
AAACCGGGGA
GATCGTGTGG
GATAAGGGCC
GGGATTTTGC
CACCGTGCGG
AAAGTGCTGA
GCATGCCCCA
AGTGAATATC
GTGAAAAAGA
CCGAGGTGCA
GACAGGCGGC
TTCAGCAAAG
AGTCTATCCT
GCCCAAGAGG
AACAGCGATA
AGCTGATCGC
CAGAAAGAAG
GACTGGGACC
CTAAGAAGTA
CGGCGGCTTC
GACAGCCCCA
CCGTGGCCTA
TTCTGTGCTG
GTGGTGGCCA
AAGTGGAAAA
GGGCAAGTCC
AAGAAACTGA
AGAGTGTGAA
AGAGCTGCTG
GGGATCACCA
TCATGGAAAG
AAGCAGCTTC
GAGAAGAATC
CCATCGACTT
TCTGGAAGCC
AAGGGCTACA
AAGAAGTGAA
AAAGGACCTG
ATCATCAAGC
TGCCTAAGTA
CTCCCTGTTC
GAGCTGGAAA
ACGGCCGGAA
GAGAATGCTG
GCCTCTGCCG
GCGAACTGCA
GAAGGGAAAC
GAACTGGCCC
TGCCCTCCAA
ATATGTGAAC
TTCCTGTACC
TGGCCAGCCA
CTATGAGAAG
CTGAAGGGCT
CCCCCGAGGA
TAATGAGCAG
AAACAGCTGT
TTGTGGAACA
GCACAAGCAC
TACCTGGACG
AGATCATCGA
GCAGATCAGC
GAGTTCTCCA
AGAGAGTGAT
CCTGGCCGAC
GCTAATCTGG
ACAAAGTGCT
GTCCGCCTAC
AACAAGCACC
GGGATAAGCC
CATCAGAGAG
CAGGCCGAGA
ATATCATCCA
CCTGTTTACC
CTGACCAATC
TGGGAGCCCC
TGCCGCCTTC
AAGTACTTTG
ACACCACCAT
CGACCGGAAG
AGGTACACCA
GCACCAAAGA
GGTGCTGGAC
GCCACCCTGA
TCCACCAGAG
CATCACCGGC
CTGTACGAGA
CACGGATCGA
CCTGTCTCAG
CTGGGAGGCG
ACAAAAGGCC
GGCGGCCACG
AAAAAGGCCG
GCCAGGCAAA
AAAGAAAAAG
GAATTCGGCA
GTGGAGAGGG
CAGAGGAAGT
CTGCTAACAT
GCGGTGACGT
CGAGGAGAAT
CCTGGCCCAG
TGAGCAAGGG
CGAGGAGCTG
TTCACCGGGG
TGGTGCCCAT
CCTGGTCGAG
CTGGACGGCG
ACGTAAACGG
CCACAAGTTC
AGCGTGTCCG
GCGAGGGCGA
GGGCGATGCC
ACCTACGGCA
AGCTGACCCT
GAAGTTCATC
TGCACCACCG
GCAAGCTGCC
CGTGCCCTGG
CCCACCCTCG
TGACCACCCT
GACCTACGGC
GTGCAGTGCT
TCAGCCGCTA
CCCCGACCAC
ATGAAGCAGC
ACGACTTCTT
CAAGTCCGCC
ATGCCCGAAG
GCTACGTCCA
GGAGCGCACC
ATCTTCTTCA
AGGACGACGG
CAACTACAAG
ACCCGCGCCG
AGGTGAAGTT
CGAGGGCGAC
ACCCTGGTGA
ACCGCATCGA
GCTGAAGGGC
ATCGACTTCA
AGGAGGACGG
CAACATCCTG
GGGCACAAGC
TGGAGTACAA
CTACAACAGC
CACAACGTCT
ATATCATGGC
CGACAAGCAG
AAGAACGGCA
TCAAGGTGAA
CTTCAAGATC
CGCCACAACA
TCGAGGACGG
CAGCGTGCAG
CTCGCCGACC
ACTACCAGCA
GAACACCCCC
ATCGGCGACG
GCCCCGTGCT
GCTGCCCGAC
AACCACTACC
TGAGCACCCA
GTCCGCCCTG
AGCAAAGACC
CCAACGAGAA
GCGCGATCAC
ATGGTCCTGC
TGGAGTTCGT
GACCGCCGCC
GGGATCACTC
TCGGCATGGA
CGAGCTGTAC
AAGGAATTCT
AACTAGAGCT
CGCTGATCAG
CCTCGACTGT
GCCTTCTAGT
TGCCAGCCAT
CTGTTGTTTG
CCCCTCCCCC
GTGCCTTCCT
TGACCCTGGA
AGGTGCCACT
CCCACTGTCC
TTTCCTAATA
AAATGAGGAA
ATTGCATCGC
ATTGTCTGAG
TAGGTGTCAT
TCTATTCTGG
GGGGTGGGGT
GGGGCAGGAC
AGCAAGGGGG
AGGATTGGGA
AGAGAATAGC
AGGCATGCTG
GGGAGCGGCC
GCAGGAACCC
CTAGTGATGG
AGTTGGCCAC
TCCCTCTCTG
CGCGCTCGCT
CGCTCACTGA
GGCCGGGCGA
CCAAAGGTCG
CCCGACGCCC
GGGCTTTGCC
CGGGCGGCCT
CAGTGAGCGA
GCGAGCGCGC
AGCTGCCTGC
AGGGGCGCCT
GATGCGGTAT
TTTCTCCTTA
CGCATCTGTG
CGGTATTTCA
CACCGCATAC
GTCAAAGCAA
CCATAGTACG
CGCCCTGTAG
CGGCGCATTA
AGCGCGGCGG
GTGTGGTGGT
TACGCGCAGC
GTGACCGCTA
CACTTGCCAG
CGCCCTAGCG
CCCGCTCCTT
TCGCTTTCTT
CCCTTCCTTT
CTCGCCACGT
TCGCCGGCTT
TCCCCGTCAA
GCTCTAAATC
GGGGGCTCCC
TTTAGGGTTC
CGATTTAGTG
CTTTACGGCA
CCTCGACCCC
AAAAAACTTG
ATTTGGGTGA
TGGTTCACGT
AGTGGGCCAT
CGCCCTGATA
GACGGTTTTT
CGCCCTTTGA
CGTTGGAGTC
CACGTTCTTT
AATAGTGGAC
TCTTGTTCCA
AACTGGAACA
ACACTCAACC
CTATCTCGGG
CTATTCTTTT
GATTTATAAG
GGATTTTGCC
GATTTCGGCC
TATTGGTTAA
AAAATGAGCT
GATTTAACAA
AAATTTAACG
CGAATTTTAA
CAAAATATTA
ACGTTTACAA
TTTTATGGTG
CACTCTCAGT
ACAATCTGCT
CTGATGCCGC
ATAGTTAAGC
CAGCCCCGAC
ACCCGCCAAC
ACCCGCTGAC
GCGCCCTGAC
GGGCTTGTCT
GCTCCCGGCA
TCCGCTTACA
GACAAGCTGT
GACCGTCTCC
GGGAGCTGCA
TGTGTCAGAG
GTTTTCACCG
TCATCACCGA
AACGCGCGAG
ACGAAAGGGC
CTCGTGATAC
GCCTATTTTT
ATAGGTTAAT
GTCATGATAA
TAATGGTTTC
TTAGACGTCA
GGTGGCACTT
TTCGGGGAAA
TGTGCGCGGA
ACCCCTATTT
GTTTATTTTT
CTAAATACAT
TCAAATATGT
ATCCGCTCAT
GAGACAATAA
CCCTGATAAA
TGCTTCAATA
ATATTGAAAA
AGGAAGAGTA
TGAGTATTCA
ACATTTCCGT
GTCGCCCTTA
TTCCCTTTTT
TGCGGCATTT
TGCCTTCCTG
TTTTTGCTCA
CCCAGAAACG
CTGGTGAAAG
TAAAAGATGC
TGAAGATCAG
TTGGGTGCAC
GAGTGGGTTA
CATCGAACTG
GATCTCAACA
GCGGTAAGAT
CCTTGAGAGT
TTTCGCCCCG
AAGAACGTTT
TCCAATGATG
AGCACTTTTA
AAGTTCTGCT
ATGTGGCGCG
GTATTATCCC
GTATTGACGC
CGGGCAAGAG
CAACTCGGTC
GCCGCATACA
CTATTCTCAG
AATGACTTGG
TTGAGTACTC
ACCAGTCACA
GAAAAGCATC
TTACGGATGG
CATGACAGTA
AGAGAATTAT
GCAGTGCTGC
CATAACCATG
AGTGATAACA
CTGCGGCCAA
CTTACTTCTG
ACAACGATCG
GAGGACCGAA
GGAGCTAACC
GCTTTTTTGC
ACAACATGGG
GGATCATGTA
ACTCGCCTTG
ATCGTTGGGA
ACCGGAGCTG
AATGAAGCCA
TACCAAACGA
CGAGCGTGAC
ACCACGATGC
CTGTAGCAAT
GGCAACAACG
TTGCGCAAAC
TATTAACTGG
CGAACTACTT
ACTCTAGCTT
CCCGGCAACA
ATTAATAGAC
TGGATGGAGG
CGGATAAAGT
TGCAGGACCA
CTTCTGCGCT
CGGCCCTTCC
GGCTGGCTGG
TTTATTGCTG
ATAAATCTGG
AGCCGGTGAG
CGTGGAAGCC
GCGGTATCAT
TGCAGCACTG
GGGCCAGATG
GTAAGCCCTC
CCGTATCGTA
GTTATCTACA
CGACGGGGAG
TCAGGCAACT
ATGGATGAAC
GAAATAGACA
GATCGCTGAG
ATAGGTGCCT
CACTGATTAA
GCATTGGTAA
CTGTCAGACC
AAGTTTACTC
ATATATACTT
TAGATTGATT
TAAAACTTCA
TTTTTAATTT
AAAAGGATCT
AGGTGAAGAT
CCTTTTTGAT
AATCTCATGA
CCAAAATCCC
TTAACGTGAG
TTTTCGTTCC
ACTGAGCGTC
AGACCCCGTA
GAAAAGATCA
AAGGATCTTC
TTGAGATCCT
TTTTTTCTGC
GCGTAATCTG
CTGCTTGCAA
ACAAAAAAAC
CACCGCTACC
AGCGGTGGTT
TGTTTGCCGG
ATCAAGAGCT
ACCAACTCTT
TTTCCGAAGG
TAACTGGCTT
CAGCAGAGCG
CAGATACCAA
ATACTGTCCT
TCTAGTGTAG
CCGTAGTTAG
GCCACCACTT
CAAGAACTCT
GTAGCACCGC
CTACATACCT
CGCTCTGCTA
ATCCTGTTAC
CAGTGGCTGC
TGCCAGTGGC
GATAAGTCGT
GTCTTACCGG
GTTGGACTCA
AGACGATAGT
TACCGGATAA
GGCGCAGCGG
TCGGGCTGAA
CGGGGGGTTC
GTGCACACAG
CCCAGCTTGG
AGCGAACGAC
CTACACCGAA
CTGAGATACC
TACAGCGTGA
GCTATGAGAA
AGCGCCACGC
TTCCCGAAGG
GAGAAAGGCG
GACAGGTATC
CGGTAAGCGG
CAGGGTCGGA
ACAGGAGAGC
GCACGAGGGA
GCTTCCAGGG
GGAAACGCCT
GGTATCTTTA
TAGTCCTGTC
GGGTTTCGCC
ACCTCTGACT
TGAGCGTCGA
TTTTTGTGAT
GCTCGTCAGG
GGGGCGGAGC
CTATGGAAAA
ACGCCAGCAA
CGCGGCCTTT
TTACGGTTCC
TGGCCTTTTG
CTGGCCTTTT
GCTCACATGT