名称: | pREDKI |
---|---|
别称: | Plasmid#51626 |
基因长: | |
质粒宿主: | 大肠杆菌 |
质粒用途: | 基因编辑 |
片段类型: | Red |
片段物种: | |
原核抗性: | Kan |
筛选标记: | |
荧光标记: | |
启动子: | araBAD |
复制子: | pSC101 |
拷贝数: | |
感受态: | DH5a |
温度: | 30度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AAATGGTTTA
AACATTTATT
ATGACAACTT
GACGGCTACA
TCATTCACTT
TTTCTTCACA
ACCGGCACGG
AACTCGCTCG
GGCTGGCCCC
GGTGCATTTT
TTAAATACCC
GCGAGAAATA
GAGTTGATCG
TCAAAACCAA
CATTGCGACC
GACGGTGGCG
ATAGGCATCC
GGGTGGTGCT
CAAAAGCAGC
TTCGCCTGGC
TGATACGTTG
GTCCTCGCGC
CAGCTTAAGA
CGCTAATCCC
TAACTGCTGG
CGGAAAAGAT
GTGACAGACG
CGACGGCGAC
AAGCAAACAT
GCTGTGCGAC
GCTGGCGATA
TCAAAATTGC
TGTCTGCCAG
GTGATCGCTG
ATGTACTGAC
AAGCCTCGCG
TACCCGATTA
TCCATCGGTG
GATGGAGCGA
CTCGTTAATC
GCTTCCATGC
GCCGCAGTAA
CAATTGCTCA
AGCAGATTTA
TCGCCAGCAG
CTCCGAATAG
CGCCCTTCCC
CTTGCCCGGC
GTTAATGATT
TGCCCAAACA
GGTCGCTGAA
ATGCGGCTGG
TGCGCTTCAT
CCGGGCGAAA
GAACCCCGTA
TTGGCAAATA
TTGACGGCCA
GTTAAGCCAT
TCATGCCAGT
AGGCGCGCGG
ACGAAAGTAA
ACCCACTGGT
GATACCATTC
GCGAGCCTCC
GGATGACGAC
CGTAGTGATG
AATCTCTCCT
GGCGGGAACA
GCAAAATATC
ACCCGGTCGG
CAAACAAATT
CTCGTCCCTG
ATTTTTCACC
ACCCCCTGAC
CGCGAATGGT
GAGATTGAGA
ATATAACCTT
TCATTCCCAG
CGGTCGGTCG
ATAAAAAAAT
CGAGATAACC
GTTGGCCTCA
ATCGGCGTTA
AACCCGCCAC
CAGATGGGCA
TTAAACGAGT
ATCCCGGCAG
CAGGGGATCA
TTTTGCGCTT
CAGCCATACT
TTTCATACTC
CCGCCATTCA
GAGAAGAAAC
CAATTGTCCA
TATTGCATCA
GACATTGCCG
TCACTGCGTC
TTTTACTGGC
TCTTCTCGCT
AACCAAACCG
GTAACCCCGC
TTATTAAAAG
CATTCTGTAA
CAAAGCGGGA
CCAAAGCCAT
GACAAAAACG
CGTAACAAAA
GTGTCTATAA
TCACGGCAGA
AAAGTCCACA
TTGATTATTT
GCACGGCGTC
ACACTTTGCT
ATGCCATAGC
ATTTTTATCC
ATAAGATTAG
CGGATCCTAC
CTGACGCTTT
TTATCGCAAC
TCTCTACTGT
TTCTCCATAC
CCGTTTTTTT
GGGAATTCGA
GCTCTAAGGA
GGTTATAAAA
AATGGATATT
AATACTGAAA
CTGAGATCAA
GCAAAAGCAT
TCACTAACCC
CCTTTCCTGT
TTTCCTAATC
AGCCCGGCAT
TTCGCGGGCG
ATATTTTCAC
AGCTATTTCA
GGAGTTCAGC
CATGAACGCT
TATTACATTC
AGGATCGTCT
TGAGGCTCAG
AGCTGGGCGC
GTCACTACCA
GCAGCTCGCC
CGTGAAGAGA
AAGAGGCAGA
ACTGGCAGAC
GACATGGAAA
AAGGCCTGCC
CCAGCACCTG
TTTGAATCGC
TATGCATCGA
TCATTTGCAA
CGCCACGGGG
CCAGCAAAAA
ATCCATTACC
CGTGCGTTTG
ATGACGATGT
TGAGTTTCAG
GAGCGCATGG
CAGAACACAT
CCGGTACATG
GTTGAAACCA
TTGCTCACCA
CCAGGTTGAT
ATTGATTCAG
AGGTATAAAA
CGAATGAGTA
CTGCACTCGC
AACGCTGGCT
GGGAAGCTGG
CTGAACGTGT
CGGCATGGAT
TCTGTCGACC
CACAGGAACT
GATCACCACT
CTTCGCCAGA
CGGCATTTAA
AGGTGATGCC
AGCGATGCGC
AGTTCATCGC
ATTACTGATC
GTTGCCAACC
AGTACGGCCT
TAATCCGTGG
ACGAAAGAAA
TTTACGCCTT
TCCTGATAAG
CAGAATGGCA
TCGTTCCGGT
GGTGGGCGTT
GATGGCTGGT
CCCGCATCAT
CAATGAAAAC
CAGCAGTTTG
ATGGCATGGA
CTTTGAGCAG
GACAATGAAT
CCTGTACATG
CCGGATTTAC
CGCAAGGACC
GTAATCATCC
GATCTGCGTT
ACCGAATGGA
TGGATGAATG
CCGCCGCGAA
CCATTCAAAA
CTCGCGAAGG
CAGAGAAATC
ACGGGGCCGT
GGCAGTCGCA
TCCCAAACGG
ATGTTACGTC
ATAAAGCCAT
GATTCAGTGT
GCCCGTCTGG
CCTTCGGATT
TGCTGGTATC
TATGACAAGG
ATGAAGCCGA
GCGCATTGTC
GAAAATACTG
CATACACTGC
AGAACGTCAG
CCGGAACGCG
ACATCACTCC
GGTTAACGAT
GAAACCATGC
AGGAGATTAA
CACTCTGCTG
ATCGCCCTGG
ATAAAACATG
GGATGACGAC
TTATTGCCGC
TCTGTTCCCA
GATATTTCGC
CGCGACATTC
GTGCATCGTC
AGAACTGACA
CAGGCCGAAG
CAGTAAAAGC
TCTTGGATTC
CTGAAACAGA
AAGCCGCAGA
GCAGAAGGTG
GCAGCATGAC
ACCGGACATT
ATCCTGCAGC
GTACCGGGAT
CGATGTGAGA
GCTGTCGAAC
AGGGGGATGA
TGCGTGGCAC
AAATTACGGC
TCGGCGTCAT
CACCGCTTCA
GAAGTTCACA
ACGTGATAGC
AAAACCCCGC
TCCGGAAAGA
AGTGGCCTGA
CATGAAAATG
TCCTACTTCC
ACACCCTGCT
TGCTGAGGTT
TGCACCGGTG
TGGCTCCGGA
AGTTAACGCT
AAAGCACTGG
CCTGGGGAAA
ACAGTACGAG
AACGACGCCA
GAACCCTGTT
TGAATTCACT
TCCGGCGTGA
ATGTTACTGA
ATCCCCGATC
ATCTATCGCG
ACGAAAGTAT
GCGTACCGCC
TGCTCTCCCG
ATGGTTTATG
CAGTGACGGC
AACGGCCTTG
AACTGAAATG
CCCGTTTACC
TCCCGGGATT
TCATGAAGTT
CCGGCTCGGT
GGTTTCGAGG
CCATAAAGTC
AGCTTACATG
GCCCAGGTGC
AGTACAGCAT
GTGGGTGACG
CGAAAAAATG
CCTGGTACTT
TGCCAACTAT
GACCCGCGTA
TGAAGCGTGA
AGGCCTGCAT
TATGTCGTGA
TTGAGCGGGA
TGAAAAGTAC
ATGGCGAGTT
TTGACGAGAT
CGTGCCGGAG
TTCATCGAAA
AAATGGACGA
GGCACTGGCT
GAAATTGGTT
TTGTATTTGG
GGAGCAATGG
CGATGACGCA
TCCTCACGAT
AATATCCGGG
TAGGCGCAAT
CACTTTCGTC
TACTCCGTTA
CAAAGCGAGG
CTGGGTATTT
CCCGGCCTTT
CTGTTATCCG
AAATCCACTG
AAAGCACAGC
GGCTGGCTGA
GGAGATAAAT
AATAAACGAG
GGGCTGTATG
CACAAAGCAT
CTTCTGTTGA
GTTAAGAACG
AGTATCGAGA
TGGCACATAG
CCTTGCTCAA
ATTGGAATCA
GGTTTGTGCC
AATACCAGTA
GAAACAGACG
AAGAATCCAT
GGGGCATGGC
GAATTAATCT
TTCTGCGAAT
TGAGATGACG
CCACTGGCTG
GGCGTCATCC
CGGTTTCCCG
GGTAAACACC
ACCGAAAAAT
AGTTACTATC
TTCAAAGCCA
CATTCGGTCG
AAATATCACT
GATTAACAGG
CGGCTATGCT
GGAGAAGATA
TTGCGCATGA
CACACTCTGA
CCTGTCGCAG
ATATTGATTG
ATGGTCATTC
CAGTCTGCTG
GCGAAATTGC
TGACGCAAAA
CGCGCTCACT
GCACGATGCC
TCATCACAAA
ATTTATCCAG
CGCAAAGGGA
CTTTTCAGGC
TAGCCGCCAG
CCGGGTAATC
AGCTTATCCA
GCAACGTTTC
GCTGGATGTT
GGCGGCAACG
AATCACTGGT
GTAACGATGG
CGATTCAGCA
ACATCACCAA
CTGCCCGAAC
AGCAACTCAG
CCATTTCGTT
AGCAAACGGC
ACATGCTGAC
TACTTTCATG
CTCAAGCTGA
CCGATAACCT
GCCGCGCCTG
CGCCATCCCC
ATGCTACCTA
AGCGCCAGTG
TGGTTGCCCT
GCGCTGGCGT
TAAATCCCGG
AATCGCCCCC
TGCCAGTCAA
GATTCAGCTT
CAGACGCTCC
GGGCAATAAA
TAATATTCTG
CAAAACCAGA
TCGTTAACGG
AAGCGTAGGA
GTGTTTATCG
TCAGCATGAA
TGTAAAAGAG
ATCGCCACGG
GTAATGCGAT
AAGGGCGATC
GTTGAGTACA
TGCAGGCCAT
TACCGCGCCA
GACAATCACC
AGCTCACAAA
AATCATGTGT
ATGTTCAGCA
AAGACATCTT
GCGGATAACG
GTCAGCCACA
GCGACTGCCT
GCTGGTCGCT
GGCAAAAAAA
TCATCTTTGA
GAAGTTTTAA
CTGATGCGCC
ACCGTGGCTA
CCTCGGCCAG
AGAACGAAGT
TGATTATTCG
CAATATGGCG
TACAAATACG
TTGAGAAGAT
TCGCGTTATT
GCAGAAAGCC
ATCCCGTCCC
TGGCGAATAT
CACGCGGTGA
CCAGTTAAAC
TCTCGGCGAA
AAAGCGTCGA
AAAGTGGTTA
CTGTCGCTGA
ATCCACAGCG
ATAGGCGATG
TCAGTAACGC
TGGCCTCGCT
GTGGCGTAGC
AGATGTCGGG
CTTTCATCAG
TCGCAGGCGG
TTCAGGTATC
GCTGAGGCGT
CAGTCCCGTT
TGCTGCTTAA
GCTGCCGATG
TAGCGTACGC
AGTGAAAGAG
AAAATTGATC
CGCCACGGCA
TCCCAATTCA
CCTCATCGGC
AAAATGGTCC
TCCAGCCAGG
CCAGAAGCAA
GTTGAGACGT
GATGCGCTGT
TTTCCAGGTT
CTCCTGCAAA
CTGCTTTTAC
GCAGCAAGAG
CAGTAATTGC
ATAAACAAGA
TCTCGCGACT
GGCGGTCGAG
GGTAAATCAT
TTTCCCCTTC
CTGCTGTTCC
ATCTGTGCAA
CCAGCTGTCG
CACCTGCTGC
AATACGCTGT
GGTTAACGCG
CCAGTGAGAC
GGATACTGCC
CATCCAGCTC
TTGTGGCAGC
AACTGATTCA
GCCCGGCGAG
AAACTGAAAT
CGATCCGGCG
AGCGATACAG
CACATTGGTC
AGACACAGAT
TATCGGTATG
TTCATACAGA
TGCCGATCAT
GATCGCGTAC
GAAACAGACC
GTGCCACCGG
TGATGGTATA
GGGCTGCCCA
TTAAACACAT
GAATACCCGT
GCCATGTTCG
ACAATCACAA
TTTCATGAAA
ATCATGATGA
TGTTCAGGAA
AATCCGCCTG
CGGGAGCCGG
GGTTCTATCG
CCACGGACGC
GTTACCAGAC
GGAAAAAAAT
CCACACTATG
TAATACGGTC
ATACTGGCCT
CCTGATGTCG
TCAACACGGC
GAAATAGTAA
TCACGAGGTC
AGGTTCTTAC
CTTAAATTTT
CGACGGAAAA
CCACGTAAAA
AACGTCGATT
TTTCAAGATA
CAGCGTGAAT
TTTCAGGAAA
TGCGGTGAGC
ATCACATCAC
CACAATTCAG
CAAATTGTGA
ACATCATCAC
GTTCATCTTT
CCCTGGTTGC
CAATGGCCCA
TTTTCCTGTC
AGTAACGAGA
AGGTCGCGAA
TTCAGGCGCT
TTTTAGACTG
GTCGTAATGA
AATTCAGCAG
GATCACATAT
GCATCAAAAA
AACCAGGTAA
TGAACCTGGG
TCCGAACTCT
AAACTGCTGA
AAGAATACAA
ATCCCAGCTG
ATCGAACTGA
ACATCGAACA
GTTCGAAGCA
GGTATCGGTC
TGATCCTGGG
TGATGCTTAC
ATCCGTTCTC
GTGATGAAGG
TAAAACCTAC
TGTATGCAGT
TCGAGTGGAA
AAACAAAGCA
TACATGGACC
ACGTATGTCT
GCTGTACGAT
CAGTGGGTAC
TGTCTTTCAA
ACACCAAGCT
TTCAACAAAC
TGGCTAACCT
GTTCATCGTT
AACAACAAAA
AAACCATCCC
GAACAACCTG
GTTGAAAACT
ACCTGACCCC
GATGTCTCTG
GCATACTGGT
TCATGGATGA
TGGTGGTAAA
TGGGATTACA
ACAAAAACTC
TACCAACAAA
TCGATCGTAC
TGAACACCCA
GTCTTTCACT
TTCGAAGAAG
TAGAATACCT
GGTTAAGGGT
CTGCGTAACA
AATTCCAACT
GAACTGTTAC
GTAAAAATCA
ACAAAAACAA
ACCGATCATC
TACATCGATT
CTATGTCTTA
CCTGATCTTC
TACAACCTGA
TCAAACCGTA
CCTGATCCCG
CAGATGATGT
ACAAACTGCC
GAACACTATC
TCCTCCGAAA
CTTTCCTGAA
ATAATAACCA
TGGGTATGGA
CAGTTTTCCC
TTTGATATGT
AACGGTGAAC
AGTTGTTCTA
CTTTTGTTTG
TTAGTCTTGA
TGCTTCACTG
ATAGATACAA
GAGCCATAAG
AACCTCAGAT
CCTTCCGTAT
TTAGCCAGTA
TGTTCTCTAG
TGTGGTTCGT
TGTTTTTGCG
TGAGCCATGA
GAACGAACCA
TTGAGATCAT
ACTTACTTTG
CATGTCACTC
AAAAATTTTG
CCTCAAAACT
GGTGAGCTGA
ATTTTTGCAG
TTAAAGCATC
GTGTAGTGTT
TTTCTTAGTC
CGTTACGTAG
GTAGGAATCT
GATGTAATGG
TTGTTGGTAT
TTTGTCACCA
TTCATTTTTA
TCTGGTTGTT
CTCAAGTTCG
GTTACGAGAT
CCATTTGTCT
ATCTAGTTCA
ACTTGGAAAA
TCAACGTATC
AGTCGGGCGG
CCTCGCTTAT
CAACCACCAA
TTTCATATTG
CTGTAAGTGT
TTAAATCTTT
ACTTATTGGT
TTCAAAACCC
ATTGGTTAAG
CCTTTTAAAC
TCATGGTAGT
TATTTTCAAG
CATTAACATG
AACTTAAATT
CATCAAGGCT
AATCTCTATA
TTTGCCTTGT
GAGTTTTCTT
TTGTGTTAGT
TCTTTTAATA
ACCACTCATA
AATCCTCATA
GAGTATTTGT
TTTCAAAAGA
CTTAACATGT
TCCAGATTAT
ATTTTATGAA
TTTTTTTAAC
TGGAAAAGAT
AAGGCAATAT
CTCTTCACTA
AAAACTAATT
CTAATTTTTC
GCTTGAGAAC
TTGGCATAGT
TTGTCCACTG
GAAAATCTCA
AAGCCTTTAA
CCAAAGGATT
CCTGATTTCC
ACAGTTCTCG
TCATCAGCTC
TCTGGTTGCT
TTAGCTAATA
CACCATAAGC
ATTTTCCCTA
CTGATGTTCA
TCATCTGAGC
GTATTGGTTA
TAAGTGAACG
ATACCGTCCG
TTCTTTCCTT
GTAGGGTTTT
CAATCGTGGG
GTTGAGTAGT
GCCACACAGC
ATAAAATTAG
CTTGGTTTCA
TGCTCCGTTA
AGTCATAGCG
ACTAATCGCT
AGTTCATTTG
CTTTGAAAAC
AACTAATTCA
GACATACATC
TCAATTGGTC
TAGGTGATTT
TAATCACTAT
ACCAATTGAG
ATGGGCTAGT
CAATGATAAT
TACTAGTCCT
TTTCCTTTGA
GTTGTGGGTA
TCTGTAAATT
CTGCTAGACC
TTTGCTGGAA
AACTTGTAAA
TTCTGCTAGA
CCCTCTGTAA
ATTCCGCTAG
ACCTTTGTGT
GTTTTTTTTG
TTTATATTCA
AGTGGTTATA
ATTTATAGAA
TAAAGAAAGA
ATAAAAAAAG
ATAAAAAGAA
TAGATCCCAG
CCCTGTGTAT
AACTCACTAC
TTTAGTCAGT
TCCGCAGTAT
TACAAAAGGA
TGTCGCAAAC
GCTGTTTGCT
CCTCTACAAA
ACAGACCTTA
AAACCCTAAA
GGCTTAAGTA
GCACCCTCGC
AAGCTCGGTT
GCGGCCGCAA
TCGGGCAAAT
CGCTGAATAT
TCCTTTTGTC
TCCGACCATC
AGGCACCTGA
GTCGCTGTCT
TTTTCGTGAC
ATTCAGTTCG
CTGCGCTCAC
GGCTCTGGCA
GTGAATGGGG
GTAAATGGCA
CTACAGGCGC
CTTTTATGGA
TTCATGCAAG
GAAACTACCC
ATAATACAAG
AAAAGCCCGT
CACGGGCTTC
TCAGGGCGTT
TTATGGCGGG
TCTGCTATGT
GGTGCTATCT
GACTTTTTGC
TGTTCAGCAG
TTCCTGCCCT
CTGATTTTCC
AGTCTGACCA
CTTCGGATTA
TCCCGTGACA
GGTCATTCAG
ACTGGCTAAT
GCACCCAGTA
AGGCAGCGGT
ATCATCAACG
GGGTCTGACG
CTCAGTGGAA
CGAAAACTCA
CGTTAAGGGA
TTTTGGTCAT
GAGATTATCA
AAAAGGATCT
TCACCTAGAT
CCTTTTAAAT
TAAAAATGAA
GTTTTAAATC
AATCTAAAGT
ATATATGAGT
AAACTTGGTC
TGACAGTTAC
GTTTAAACCA
TTTCCTGCAG
GGGGGGGGGG
AAAGCCACGT
TGTGTCTCAA
AATCTCTGAT
GTTACATTGC
ACAAGATAAA
AATATATCAT
CATGAACAAT
AAAACTGTCT
GCTTACATAA
ACAGTAATAC
AAGGGGTGTT
ATGAGCCATA
TTCAACGGGA
AACGTCTTGC
TCGAGGCCGC
GATTAAATTC
CAACATGGAT
GCTGATTTAT
ATGGGTATAA
ATGGGCTCGC
GATAATGTCG
GGCAATCAGG
TGCGACAATC
TATCGATTGT
ATGGGAAGCC
CGATGCGCCA
GAGTTGTTTC
TGAAACATGG
CAAAGGTAGC
GTTGCCAATG
ATGTTACAGA
TGAGATGGTC
AGACTAAACT
GGCTGACGGA
ATTTATGCCT
CTTCCGACCA
TCAAGCATTT
TATCCGTACT
CCTGATGATG
CATGGTTACT
CACCACTGCG
ATCCCCGGGA
AAACAGCATT
CCAGGTATTA
GAAGAATATC
CTGATTCAGG
TGAAAATATT
GTTGATGCGC
TGGCAGTGTT
CCTGCGCCGG
TTGCATTCGA
TTCCTGTTTG
TAATTGTCCT
TTTAACAGCG
ATCGCGTATT
TCGTCTCGCT
CAGGCGCAAT
CACGAATGAA
TAACGGTTTG
GTTGATGCGA
GTGATTTTGA
TGACGAGCGT
AATGGCTGGC
CTGTTGAACA
AGTCTGGAAA
GAAATGCATA
AGCTTTTGCC
ATTCTCACCG
GATTCAGTCG
TCACTCATGG
TGATTTCTCA
CTTGATAACC
TTATTTTTGA
CGAGGGGAAA
TTAATAGGTT
GTATTGATGT
TGGACGAGTC
GGAATCGCAG
ACCGATACCA
GGATCTTGCC
ATCCTATGGA
ACTGCCTCGG
TGAGTTTTCT
CCTTCATTAC
AGAAACGGCT
TTTTCAAAAA
TATGGTATTG
ATAATCCTGA
TATGAATAAA
TTGCAGTTTC
ATTTGATGCT
CGATGAGTTT
TTCTAATCAG
AATTGGTTAA
TTGGTTGG