名称: | pAAV-ZsGreen1-CD68(mouse)-shRNA1 |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞,腺相关病毒 |
质粒用途: | 干扰沉默 |
片段类型: | shRNA |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 绿色 |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CCTGCAGGCA
GCTGCGCGCT
CGCTCGCTCA
CTGAGGCCGC
CCGGGCAAAG
CCCGGGCGTC
GGGCGACCTT
TGGTCGCCCG
GCCTCAGTGA
GCGAGCGAGC
GCGCAGAGAG
GGAGTGGCCA
ACTCCATCAC
TAGGGGTTCC
TGCGGCCGCA
CGCGTGGAGC
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGTCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCG
TTTAGTGAAC
CGTCAGATCG
CCTGGAGACG
CCATCCACGC
TGTTTTGACC
TCCATAGAAG
ACACCGGGAC
CGATCCAGCC
TCCGCGGATT
CGAATCCCGG
CCGGGAACGG
TGCATTGGAA
CGCGGATTCC
CCGTGCCAAG
AGTGACGTAA
GTACCGCCTA
TAGAGTCTAT
AGGCCCACAA
AAAATGCTTT
CTTCTTTTAA
TATACTTTTT
TGTTTATCTT
ATTTCTAATA
CTTTCCCTAA
TCTCTTTCTT
TCAGGGCAAT
AATGATACAA
TGTATCATGC
CTCTTTGCAC
CATTCTAAAG
AATAACAGTG
ATAATTTCTG
GGTTAAGGCA
ATAGCAATAT
TTCTGCATAT
AAATATTTCT
GCATATAAAT
TGTAACTGAT
GTAAGAGGTT
TCATATTGCT
AATAGCAGCT
ACAATCCAGC
TACCATTCTG
CTTTTATTTT
ATGGTTGGGA
TAAGGCTGGA
TTATTCTGAG
TCCAAGCTAG
GCCCTTTTGC
TAATCATGTT
CATACCTCTT
ATCTTCCTCC
CACAGCTCCT
GGGCAACGTG
CTGGTCTGTG
TGCTGGCCCA
TCACTTTGGC
AAAGAATTGG
GATTCGAACA
TCGATTGAAT
TCCACCATGG
CCCAGTCCAA
GCACGGCCTG
ACCAAGGAGA
TGACCATGAA
GTACCGCATG
GAGGGCTGCG
TGGACGGCCA
CAAGTTCGTG
ATCACCGGCG
AGGGCATCGG
CTACCCCTTC
AAGGGCAAGC
AGGCCATCAA
CCTGTGCGTG
GTGGAGGGCG
GCCCCTTGCC
CTTCGCCGAG
GACATCTTGT
CCGCCGCCTT
CATGTACGGC
AACCGCGTGT
TCACCGAGTA
CCCCCAGGAC
ATCGTCGACT
ACTTCAAGAA
CTCCTGCCCC
GCCGGCTACA
CCTGGGACCG
CTCCTTCCTG
TTCGAGGACG
GCGCCGTGTG
CATCTGCAAC
GCCGACATCA
CCGTGAGCGT
GGAGGAGAAC
TGCATGTACC
ACGAGTCCAA
GTTCTACGGC
GTGAACTTCC
CCGCCGACGG
CCCCGTGATG
AAGAAGATGA
CCGACAACTG
GGAGCCCTCC
TGCGAGAAGA
TCATCCCCGT
GCCCAAGCAG
GGCATCTTGA
AGGGCGACGT
GAGCATGTAC
CTGCTGCTGA
AGGACGGTGG
CCGCTTGCGC
TGCCAGTTCG
ACACCGTGTA
CAAGGCCAAG
TCCGTGCCCC
GCAAGATGCC
CGACTGGCAC
TTCATCCAGC
ACAAGCTGAC
CCGCGAGGAC
CGCAGCGACG
CCAAGAACCA
GAAGTGGCAC
CTGACCGAGC
ACGCCATCGC
CTCCGGCTCC
GCCTTGCCCT
AAAGATCTAC
GGGTGGCATC
CCTGTGACCC
CTCCCCAGTG
CCTCTCCTGG
CCCTGGAAGT
TGCCACTCCA
GTGCCCACCA
GCCTTGTCCT
AATAAAATTA
AGTTGCATCA
TTTTGTCTGA
CTAGGTGTCC
TTCTATAATA
TTATGGGGTG
GAGGGGGGTG
GTATGGAGCA
AGGGGCAAGT
TGGGAAGACA
ACCTGTAGGG
CCTGCGGGGT
CTATTGGGAA
CCAAGCTGGA
GTGCAGTGGC
ACAATCTTGG
CTCACTGCAA
TCTCCGCCTC
CTGGGTTCAA
GCGATTCTCC
TGCCTCAGCC
TCCCGAGTTG
TTGGGATTCC
AGGCATGCAT
GACCAGGCTC
AGCTAATTTT
TGTTTTTTTG
GTAGAGACGG
GGTTTCACCA
TATTGGCCAG
GCTGGTCTCC
AACTCCTAAT
CTCAGGTGAT
CTACCCACCT
TGGCCTCCCA
AATTGCTGGG
ATTACAGGCG
TGAACCACTG
CTCCCTTCCC
TGTCCTTCTG
ATTTTGTAGG
TAACCGACCT
GTTGGTGAGT
AGATGCTTTC
AGGGAAAGAA
AGGAAGACAA
GTTAAGTTAG
TCCAAGGTGA
TTCCCTCTCC
AAAGGGCTGA
CTGTGTGCCC
TTTTCAGGAA
CGTAGCAACT
TGGAAGAAAA
CCAGGCGTGC
CTGAGGTCTC
TGGTGGACAA
GAAACTCTTG
TTTCCTTTGC
CCTCCTCTGG
CTGCCTACAT
GTATGCCTAC
TTAATTAGGA
TAGATTGTAT
CCTCTGATGG
CTATATTCTT
TCCTGACCTA
GGCCAGAGGC
ATTGATGTGC
AGCAGGTCTC
CTTAGTCATC
AACTATGACC
TTCCCACCAA
CAGGGAAAAC
TACATCCACA
GGTAAGTCTA
GGTCTGACAG
ACTAAACAAC
CCCTATGCTG
AAACCCAAGT
GTTCCATCAT
CCTTTTTACC
CCCCTCAGAA
TCGGTCGAGG
TGGTCGGTTT
GGTCGTAAGG
GTGTGGCTAT
TAACATGGTG
ACCGAAGAAG
ACAAGAGGAC
TCTTCGAGAC
ATTGAGACTT
TCTACAACAC
CTCCATTGAA
GAGATGCCCC
TCAACGTTGC
TGACCTCATT
TGAGGGGCTG
TCCTGCGACC
TGGCCCTAGC
CCAGGGTTCA
GTCCTGGGGT
GGGGCTAAGG
AAGAGCTGGA
GGGGGGAGGG
GAGGGAGCCA
AGGGATGGAC
ATCTTGTTTT
TGTTTTGGCT
TTTTTTTTTT
TTGTTTCAGT
TTTTTTTCTC
TATGAATAAA
TGTCACTTTT
TGAGGCAAAA
AGAAGGAACC
GTGAACATTT
TAGACACCCT
TTTCTTTGGG
GTAGGCTCTG
CCCCAGGCGC
CGTCTCCTTC
CCCCCCCAAA
CACTAATGCA
TTTCCCTAAC
CTAGTCACCT
CGTTCCTAAA
GGCTTTCCTA
CCCCAGCCAA
ATCTCCAAAA
GTGAGTCAAG
GGGCTAAAAA
ACAAGGCTGG
CCTCATTTGC
TGGACCAAAT
CTACAGGGAG
AACCCCTGAG
TGAGGTTGTC
CAGGGAATTG
TCCCCTGGTG
AGGGGAGCAG
GGGAGAGAAA
ATGGTAGCCA
TTTTTACATT
GTTTTGTATA
GTATTTATTG
ATTCAGGAAA
CAAACACAAA
ATTCTGAATA
AAATTACTTG
GAAACTGCCT
GTTTGGGCTT
CTTGTTTCTT
TCCCTTCCCT
CCTACCTGCT
AGTGTGGATC
ACCTGCCCCC
ATACTAGTTG
ATTACTGAAT
TTGCCATGTT
GCTTCCTGCA
ATACCAAATG
ACCCACATTA
CTAACATTTG
GTAATTTGCC
TCAGTGTTAG
GCACAAGACA
TGAGTGAAAC
TTGCTGCATT
AAGACTTAAA
CTGGCTGGGT
ATGGTGGCGC
AAGCCTTTAA
TCCCAGCATT
CTGCCGGAGG
CAGAAGCAGG
TGGATTTCTG
AGTTCGAGGC
TAGCCTGGTC
TACAAAAGTG
AGTTCCAGGA
CAGCCAAGGC
TACACAAAAT
ACCTGTCTTG
AAAAACCAAA
AAAAAAAAAT
TCAACTGGTT
GGTTAGGCCT
AGCCCAGATG
ATGTAGGTGT
CAACTCACCT
TGGGGATGTA
GGGAGATGGT
TGTTCTGTAG
CTCTTCCTGT
CCTGCAACTA
AATAAATAGG
CTAAGCTGAC
CTCTTACATC
TGCCTTCGGC
CTCCTGTGCT
AGGATTGGAG
TGCATGGGTG
GGGTTGCATT
TCTTACCAAT
GAGGAAAGGG
CTTCCCATTT
CTCTGCTGCA
TTGTAAGCTC
CCAAGTAGAG
CAAGTTTTGC
TTAGGTAAGT
TCCGTGTGAG
TCAGCTGCCT
CATTCTCACG
AGGTAACCAA
GGCTTTGTAC
CGCCCACTGA
GAACGTCACT
GCCAATCACA
GCCTAATTGT
GAAAACCAAT
GGCTTGAGTG
GGTTGCTAAA
GCTGAGGTGT
CTGAGTCAGG
TTTGGGGTGG
GATTATTTTA
GTTAAGGGAA
GTGAGGCTTT
TCATTTCCTC
TTCCAAGAGA
AGGCAAAGGG
GATTGGATTG
AGGAAGGAAC
TGGTGTAGCC
TAGCTGGTCT
GAGCATCTCT
GCCGGATCCG
CACTGTGTGT
GTGTGCCACA
CTCGAGTGTG
GCACACACAC
ACAGTGCTTT
TTAAGCTTGC
GGCCGCAGGA
ACCCCTAGTG
ATGGAGTTGG
CCACTCCCTC
TCTGCGCGCT
CGCTCGCTCA
CTGAGGCCGG
GCGACCAAAG
GTCGCCCGAC
GCCCGGGCTT
TGCCCGGGCG
GCCTCAGTGA
GCGAGCGAGC
GCGCAGCTGC
CTGCAGGGGC
GCCTGATGCG
GTATTTTCTC
CTTACGCATC
TGTGCGGTAT
TTCACACCGC
ATACGTCAAA
GCAACCATAG
TACGCGCCCT
GTAGCGGCGC
ATTAAGCGCG
GCGGGTGTGG
TGGTTACGCG
CAGCGTGACC
GCTACACTTG
CCAGCGCCCT
AGCGCCCGCT
CCTTTCGCTT
TCTTCCCTTC
CTTTCTCGCC
ACGTTCGCCG
GCTTTCCCCG
TCAAGCTCTA
AATCGGGGGC
TCCCTTTAGG
GTTCCGATTT
AGTGCTTTAC
GGCACCTCGA
CCCCAAAAAA
CTTGATTTGG
GTGATGGTTC
ACGTAGTGGG
CCATCGCCCT
GATAGACGGT
TTTTCGCCCT
TTGACGTTGG
AGTCCACGTT
CTTTAATAGT
GGACTCTTGT
TCCAAACTGG
AACAACACTC
AACCCTATCT
CGGGCTATTC
TTTTGATTTA
TAAGGGATTT
TGCCGATTTC
GGCCTATTGG
TTAAAAAATG
AGCTGATTTA
ACAAAAATTT
AACGCGAATT
TTAACAAAAT
ATTAACGTTT
ACAATTTTAT
GGTGCACTCT
CAGTACAATC
TGCTCTGATG
CCGCATAGTT
AAGCCAGCCC
CGACACCCGC
CAACACCCGC
TGACGCGCCC
TGACGGGCTT
GTCTGCTCCC
GGCATCCGCT
TACAGACAAG
CTGTGACCGT
CTCCGGGAGC
TGCATGTGTC
AGAGGTTTTC
ACCGTCATCA
CCGAAACGCG
CGAGACGAAA
GGGCCTCGTG
ATACGCCTAT
TTTTATAGGT
TAATGTCATG
ATAATAATGG
TTTCTTAGAC
GTCAGGTGGC
ACTTTTCGGG
GAAATGTGCG
CGGAACCCCT
ATTTGTTTAT
TTTTCTAAAT
ACATTCAAAT
ATGTATCCGC
TCATGAGACA
ATAACCCTGA
TAAATGCTTC
AATAATATTG
AAAAAGGAAG
AGTATGAGTA
TTCAACATTT
CCGTGTCGCC
CTTATTCCCT
TTTTTGCGGC
ATTTTGCCTT
CCTGTTTTTG
CTCACCCAGA
AACGCTGGTG
AAAGTAAAAG
ATGCTGAAGA
TCAGTTGGGT
GCACGAGTGG
GTTACATCGA
ACTGGATCTC
AACAGCGGTA
AGATCCTTGA
GAGTTTTCGC
CCCGAAGAAC
GTTTTCCAAT
GATGAGCACT
TTTAAAGTTC
TGCTATGTGG
CGCGGTATTA
TCCCGTATTG
ACGCCGGGCA
AGAGCAACTC
GGTCGCCGCA
TACACTATTC
TCAGAATGAC
TTGGTTGAGT
ACTCACCAGT
CACAGAAAAG
CATCTTACGG
ATGGCATGAC
AGTAAGAGAA
TTATGCAGTG
CTGCCATAAC
CATGAGTGAT
AACACTGCGG
CCAACTTACT
TCTGACAACG
ATCGGAGGAC
CGAAGGAGCT
AACCGCTTTT
TTGCACAACA
TGGGGGATCA
TGTAACTCGC
CTTGATCGTT
GGGAACCGGA
GCTGAATGAA
GCCATACCAA
ACGACGAGCG
TGACACCACG
ATGCCTGTAG
CAATGGCAAC
AACGTTGCGC
AAACTATTAA
CTGGCGAACT
ACTTACTCTA
GCTTCCCGGC
AACAATTAAT
AGACTGGATG
GAGGCGGATA
AAGTTGCAGG
ACCACTTCTG
CGCTCGGCCC
TTCCGGCTGG
CTGGTTTATT
GCTGATAAAT
CTGGAGCCGG
TGAGCGTGGG
TCTCGCGGTA
TCATTGCAGC
ACTGGGGCCA
GATGGTAAGC
CCTCCCGTAT
CGTAGTTATC
TACACGACGG
GGAGTCAGGC
AACTATGGAT
GAACGAAATA
GACAGATCGC
TGAGATAGGT
GCCTCACTGA
TTAAGCATTG
GTAACTGTCA
GACCAAGTTT
ACTCATATAT
ACTTTAGATT
GATTTAAAAC
TTCATTTTTA
ATTTAAAAGG
ATCTAGGTGA
AGATCCTTTT
TGATAATCTC
ATGACCAAAA
TCCCTTAACG
TGAGTTTTCG
TTCCACTGAG
CGTCAGACCC
CGTAGAAAAG
ATCAAAGGAT
CTTCTTGAGA
TCCTTTTTTT
CTGCGCGTAA
TCTGCTGCTT
GCAAACAAAA
AAACCACCGC
TACCAGCGGT
GGTTTGTTTG
CCGGATCAAG
AGCTACCAAC
TCTTTTTCCG
AAGGTAACTG
GCTTCAGCAG
AGCGCAGATA
CCAAATACTG
TCCTTCTAGT
GTAGCCGTAG
TTAGGCCACC
ACTTCAAGAA
CTCTGTAGCA
CCGCCTACAT
ACCTCGCTCT
GCTAATCCTG
TTACCAGTGG
CTGCTGCCAG
TGGCGATAAG
TCGTGTCTTA
CCGGGTTGGA
CTCAAGACGA
TAGTTACCGG
ATAAGGCGCA
GCGGTCGGGC
TGAACGGGGG
GTTCGTGCAC
ACAGCCCAGC
TTGGAGCGAA
CGACCTACAC
CGAACTGAGA
TACCTACAGC
GTGAGCTATG
AGAAAGCGCC
ACGCTTCCCG
AAGGGAGAAA
GGCGGACAGG
TATCCGGTAA
GCGGCAGGGT
CGGAACAGGA
GAGCGCACGA
GGGAGCTTCC
AGGGGGAAAC
GCCTGGTATC
TTTATAGTCC
TGTCGGGTTT
CGCCACCTCT
GACTTGAGCG
TCGATTTTTG
TGATGCTCGT
CAGGGGGGCG
GAGCCTATGG
AAAAACGCCA
GCAACGCGGC
CTTTTTACGG
TTCCTGGCCT
TTTGCTGGCC
TTTTGCTCAC
ATGT