名称: | pCAMBIA1303-gpdA-GUS-Linker-CtnD-TrpC-Hygro |
---|---|
别称: | |
基因长: | |
质粒宿主: | 丝状真菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | |
原核抗性: | Kan |
筛选标记: | Hyg |
荧光标记: | Gus |
启动子: | gpdA |
复制子: | pVS1 |
拷贝数: | 低 |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AGATCTGACT
AGTTTACGTC
CTGTAGAAAC
CCCAACCCGT
GAAATCAAAA
AACTCGACGG
CCTGTGGGCA
TTCAGTCTGG
ATCGCGAAAA
CTGTGGAATT
GATCAGCGTT
GGTGGGAAAG
CGCGTTACAA
GAAAGCCGGG
CAATTGCTGT
GCCAGGCAGT
TTTAACGATC
AGTTCGCCGA
TGCAGATATT
CGTAATTATG
CGGGCAACGT
CTGGTATCAG
CGCGAAGTCT
TTATACCGAA
AGGTTGGGCA
GGCCAGCGTA
TCGTGCTGCG
TTTCGATGCG
GTCACTCATT
ACGGCAAAGT
GTGGGTCAAT
AATCAGGAAG
TGATGGAGCA
TCAGGGCGGC
TATACGCCAT
TTGAAGCCGA
TGTCACGCCG
TATGTTATTG
CCGGGAAAAG
TGTACGTATC
ACCGTTTGTG
TGAACAACGA
ACTGAACTGG
CAGACTATCC
CGCCGGGAAT
GGTGATTACC
GACGAAAACG
GCAAGAAAAA
GCAGTCTTAC
TTCCATGATT
TCTTTAACTA
TGCCGGAATC
CATCGCAGCG
TAATGCTCTA
CACCACGCCG
AACACCTGGG
TGGACGATAT
CACCGTGGTG
ACGCATGTCG
CGCAAGACTG
TAACCACGCG
TCTGTTGACT
GGCAGGTGGT
GGCCAATGGT
GATGTCAGCG
TTGAACTGCG
TGATGCGGAT
CAACAGGTGG
TTGCAACTGG
ACAAGGCACT
AGCGGGACTT
TGCAAGTGGT
GAATCCGCAC
CTCTGGCAAC
CGGGTGAAGG
TTATCTCTAT
GAACTGTGCG
TCACAGCCAA
AAGCCAGACA
GAGTGTGATA
TCTACCCGCT
TCGCGTCGGC
ATCCGGTCAG
TGGCAGTGAA
GGGCCAACAG
TTCCTGATTA
ACCACAAACC
GTTCTACTTT
ACTGGCTTTG
GTCGTCATGA
AGATGCGGAC
TTACGTGGCA
AAGGATTCGA
TAACGTGCTG
ATGGTGCACG
ACCACGCATT
AATGGACTGG
ATTGGGGCCA
ACTCCTACCG
TACCTCGCAT
TACCCTTACG
CTGAAGAGAT
GCTCGACTGG
GCAGATGAAC
ATGGCATCGT
GGTGATTGAT
GAAACTGCTG
CTGTCGGCTT
TCAGCTGTCT
TTAGGCATTG
GTTTCGAAGC
GGGCAACAAG
CCGAAAGAAC
TGTACAGCGA
AGAGGCAGTC
AACGGGGAAA
CTCAGCAAGC
GCACTTACAG
GCGATTAAAG
AGCTGATAGC
GCGTGACAAA
AACCACCCAA
GCGTGGTGAT
GTGGAGTATT
GCCAACGAAC
CGGATACCCG
TCCGCAAGGT
GCACGGGAAT
ATTTCGCGCC
ACTGGCGGAA
GCAACGCGTA
AACTCGACCC
GACGCGTCCG
ATCACCTGCG
TCAATGTAAT
GTTCTGCGAC
GCTCACACCG
ATACCATCAG
CGATCTCTTT
GATGTGCTGT
GCCTGAACCG
TTATTACGGA
TGGTATGTCC
AAAGCGGCGA
TTTGGAAACG
GCAGAGAAGG
TACTGGAAAA
AGAACTTCTG
GCCTGGCAGG
AGAAACTGCA
TCAGCCGATT
ATCATCACCG
AATACGGCGT
GGATACGTTA
GCCGGGCTGC
ACTCAATGTA
CACCGACATG
TGGAGTGAAG
AGTATCAGTG
TGCATGGCTG
GATATGTATC
ACCGCGTCTT
TGATCGCGTC
AGCGCCGTCG
TCGGTGAACA
GGTATGGAAT
TTCGCCGATT
TTGCGACCTC
GCAAGGCATA
TTGCGCGTTG
GCGGTAACAA
GAAAGGGATC
TTCACTCGCG
ACCGCAAACC
GAAGTCGGCG
GCTTTTCTGC
TGCAAAAACG
CTGGACTGGC
ATGAACTTCG
GTGAAAAACC
GCAGCAGGGA
GGCAAACAAG
CTGGTGGAGG
AGGTTCCGGC
GGTGGCGGTT
CCGGTGGCGG
AGGTTCCATG
CCTCCTATCA
TCCATTGCGT
CCGCCACGCC
CAGGCAATCC
ACAATCTCTC
TGTCGCAAAC
CACGTTATCC
CCGATCCCAT
CCTCACAGAT
CTGGGCAACG
AACAATGCCG
CAAGCTCCGT
GAGAAGTTTC
CTTACCATTC
CGACGTGGAA
TTGGTCGTCT
CCTCGCCCCT
GCGTCGCACG
ATCGCCACAA
GCCTCCAGGG
CTTCGAGCCC
GTCTTCCAGT
CGCGGGAGGG
GCTGAAGTTG
ATCGTTCATC
CGGATCTCCA
GGAGACGAGC
GATGTTCCCT
GTGATACGGG
GAGTAATCCG
GAGGTTTTGA
GGGAGGAGAT
TGAGAAGGGT
GGGCTTCCGG
TTGATTTGGG
GTTGCTGTTT
GATGGGTGGA
ACAGTAAGAA
AGGACCGTAT
GCGCCTACCA
ACAAGGAGAT
CAAGAATCGA
GCCCGTGCTG
CCCGTCGGTG
GCTGAAGGCA
CGGCCGGAAA
AGGTGATTGT
TGTCGTTACC
CATGGTGGAT
TCTTGCACTA
TTTCACCGAG
GACTGGGAGG
ATAGCAGTGA
ATACCAGGGG
ACCGGCTGGG
CCAATACCGA
ATTCCGCACG
TTCGAGTTTG
CCGATGTCGA
GCATAAAGAT
GATCTGGAAG
GCTACGGGTT
GGACGGTGAC
AACGCTACGT
TGATCGAGAC
GGTTGAATCT
CGCCGACGTC
GTGGGAAGGA
TGGCACAACA
CCCAGCCGTG
AGCAGCAGAA
GGTTCTGTAC
AAGCTTGGGG
TTCAGGGCTG
GGATAACCAG
GGCCTCGCGC
TTAGTGTAGC
TGAGAGAGAG
AAGACCAAGG
TGCCTCAAGG
CGAGGAGGCA
CAAAGCAAGC
AGTCAGCAAT
GCGTAATCTC
GATGCTGGAT
TCTCCATTGA
AAAAGAAGCT
AGCCACCACC
ACCACCACCA
CGTGTGAATT
GGTGACCAGC
TCGAATTTCC
CCGATCGTTC
AAACATTTGG
CAATAAAGTT
TCTTAAGATT
GAATCCTGTT
GCCGGTCTTG
CGATGATTAT
CATATAATTT
CTGTTGAATT
ACGTTAAGCA
TGTAATAATT
AACATGTAAT
GCATGACGTT
ATTTATGAGA
TGGGTTTTTA
TGATTAGAGT
CCCGCAATTA
TACATTTAAT
ACGCGATAGA
AAACAAAATA
TAGCGCGCAA
ACTAGGATAA
ATTATCGCGC
GCGGTGTCAT
CTATGTTACT
AGATCGGGAA
TTAAACTATC
AGTGTTTGAC
AGGATATATT
GGCGGGTAAA
CCTAAGAGAA
AAGAGCGTTT
ATTAGAATAA
CGGATATTTA
AAAGGGCGTG
AAAAGGTTTA
TCCGTTCGTC
CATTTGTATG
TGCATGCCAA
CCACAGGGTT
CCCCTCGGGA
TCAAAGTACT
TTGATCCAAC
CCCTCCGCTG
CTATAGTGCA
GTCGGCTTCT
GACGTTCAGT
GCAGCCGTCT
TCTGAAAACG
ACATGTCGCA
CAAGTCCTAA
GTTACGCGAC
AGGCTGCCGC
CCTGCCCTTT
TCCTGGCGTT
TTCTTGTCGC
GTGTTTTAGT
CGCATAAAGT
AGAATACTTG
CGACTAGAAC
CGGAGACATT
ACGCCATGAA
CAAGAGCGCC
GCCGCTGGCC
TGCTGGGCTA
TGCCCGCGTC
AGCACCGACG
ACCAGGACTT
GACCAACCAA
CGGGCCGAAC
TGCACGCGGC
CGGCTGCACC
AAGCTGTTTT
CCGAGAAGAT
CACCGGCACC
AGGCGCGACC
GCCCGGAGCT
GGCCAGGATG
CTTGACCACC
TACGCCCTGG
CGACGTTGTG
ACAGTGACCA
GGCTAGACCG
CCTGGCCCGC
AGCACCCGCG
ACCTACTGGA
CATTGCCGAG
CGCATCCAGG
AGGCCGGCGC
GGGCCTGCGT
AGCCTGGCAG
AGCCGTGGGC
CGACACCACC
ACGCCGGCCG
GCCGCATGGT
GTTGACCGTG
TTCGCCGGCA
TTGCCGAGTT
CGAGCGTTCC
CTAATCATCG
ACCGCACCCG
GAGCGGGCGC
GAGGCCGCCA
AGGCCCGAGG
CGTGAAGTTT
GGCCCCCGCC
CTACCCTCAC
CCCGGCACAG
ATCGCGCACG
CCCGCGAGCT
GATCGACCAG
GAAGGCCGCA
CCGTGAAAGA
GGCGGCTGCA
CTGCTTGGCG
TGCATCGCTC
GACCCTGTAC
CGCGCACTTG
AGCGCAGCGA
GGAAGTGACG
CCCACCGAGG
CCAGGCGGCG
CGGTGCCTTC
CGTGAGGACG
CATTGACCGA
GGCCGACGCC
CTGGCGGCCG
CCGAGAATGA
ACGCCAAGAG
GAACAAGCAT
GAAACCGCAC
CAGGACGGCC
AGGACGAACC
GTTTTTCATT
ACCGAAGAGA
TCGAGGCGGA
GATGATCGCG
GCCGGGTACG
TGTTCGAGCC
GCCCGCGCAC
GTCTCAACCG
TGCGGCTGCA
TGAAATCCTG
GCCGGTTTGT
CTGATGCCAA
GCTGGCGGCC
TGGCCGGCCA
GCTTGGCCGC
TGAAGAAACC
GAGCGCCGCC
GTCTAAAAAG
GTGATGTGTA
TTTGAGTAAA
ACAGCTTGCG
TCATGCGGTC
GCTGCGTATA
TGATGCGATG
AGTAAATAAA
CAAATACGCA
AGGGGAACGC
ATGAAGGTTA
TCGCTGTACT
TAACCAGAAA
GGCGGGTCAG
GCAAGACGAC
CATCGCAACC
CATCTAGCCC
GCGCCCTGCA
ACTCGCCGGG
GCCGATGTTC
TGTTAGTCGA
TTCCGATCCC
CAGGGCAGTG
CCCGCGATTG
GGCGGCCGTG
CGGGAAGATC
AACCGCTAAC
CGTTGTCGGC
ATCGACCGCC
CGACGATTGA
CCGCGACGTG
AAGGCCATCG
GCCGGCGCGA
CTTCGTAGTG
ATCGACGGAG
CGCCCCAGGC
GGCGGACTTG
GCTGTGTCCG
CGATCAAGGC
AGCCGACTTC
GTGCTGATTC
CGGTGCAGCC
AAGCCCTTAC
GACATATGGG
CCACCGCCGA
CCTGGTGGAG
CTGGTTAAGC
AGCGCATTGA
GGTCACGGAT
GGAAGGCTAC
AAGCGGCCTT
TGTCGTGTCG
CGGGCGATCA
AAGGCACGCG
CATCGGCGGT
GAGGTTGCCG
AGGCGCTGGC
CGGGTACGAG
CTGCCCATTC
TTGAGTCCCG
TATCACGCAG
CGCGTGAGCT
ACCCAGGCAC
TGCCGCCGCC
GGCACAACCG
TTCTTGAATC
AGAACCCGAG
GGCGACGCTG
CCCGCGAGGT
CCAGGCGCTG
GCCGCTGAAA
TTAAATCAAA
ACTCATTTGA
GTTAATGAGG
TAAAGAGAAA
ATGAGCAAAA
GCACAAACAC
GCTAAGTGCC
GGCCGTCCGA
GCGCACGCAG
CAGCAAGGCT
GCAACGTTGG
CCAGCCTGGC
AGACACGCCA
GCCATGAAGC
GGGTCAACTT
TCAGTTGCCG
GCGGAGGATC
ACACCAAGCT
GAAGATGTAC
GCGGTACGCC
AAGGCAAGAC
CATTACCGAG
CTGCTATCTG
AATACATCGC
GCAGCTACCA
GAGTAAATGA
GCAAATGAAT
AAATGAGTAG
ATGAATTTTA
GCGGCTAAAG
GAGGCGGCAT
GGAAAATCAA
GAACAACCAG
GCACCGACGC
CGTGGAATGC
CCCATGTGTG
GAGGAACGGG
CGGTTGGCCA
GGCGTAAGCG
GCTGGGTTGT
CTGCCGGCCC
TGCAATGGCA
CTGGAACCCC
CAAGCCCGAG
GAATCGGCGT
GACGGTCGCA
AACCATCCGG
CCCGGTACAA
ATCGGCGCGG
CGCTGGGTGA
TGACCTGGTG
GAGAAGTTGA
AGGCCGCGCA
GGCCGCCCAG
CGGCAACGCA
TCGAGGCAGA
AGCACGCCCC
GGTGAATCGT
GGCAAGCGGC
CGCTGATCGA
ATCCGCAAAG
AATCCCGGCA
ACCGCCGGCA
GCCGGTGCGC
CGTCGATTAG
GAAGCCGCCC
AAGGGCGACG
AGCAACCAGA
TTTTTTCGTT
CCGATGCTCT
ATGACGTGGG
CACCCGCGAT
AGTCGCAGCA
TCATGGACGT
GGCCGTTTTC
CGTCTGTCGA
AGCGTGACCG
ACGAGCTGGC
GAGGTGATCC
GCTACGAGCT
TCCAGACGGG
CACGTAGAGG
TTTCCGCAGG
GCCGGCCGGC
ATGGCCAGTG
TGTGGGATTA
CGACCTGGTA
CTGATGGCGG
TTTCCCATCT
AACCGAATCC
ATGAACCGAT
ACCGGGAAGG
GAAGGGAGAC
AAGCCCGGCC
GCGTGTTCCG
TCCACACGTT
GCGGACGTAC
TCAAGTTCTG
CCGGCGAGCC
GATGGCGGAA
AGCAGAAAGA
CGACCTGGTA
GAAACCTGCA
TTCGGTTAAA
CACCACGCAC
GTTGCCATGC
AGCGTACGAA
GAAGGCCAAG
AACGGCCGCC
TGGTGACGGT
ATCCGAGGGT
GAAGCCTTGA
TTAGCCGCTA
CAAGATCGTA
AAGAGCGAAA
CCGGGCGGCC
GGAGTACATC
GAGATCGAGC
TAGCTGATTG
GATGTACCGC
GAGATCACAG
AAGGCAAGAA
CCCGGACGTG
CTGACGGTTC
ACCCCGATTA
CTTTTTGATC
GATCCCGGCA
TCGGCCGTTT
TCTCTACCGC
CTGGCACGCC
GCGCCGCAGG
CAAGGCAGAA
GCCAGATGGT
TGTTCAAGAC
GATCTACGAA
CGCAGTGGCA
GCGCCGGAGA
GTTCAAGAAG
TTCTGTTTCA
CCGTGCGCAA
GCTGATCGGG
TCAAATGACC
TGCCGGAGTA
CGATTTGAAG
GAGGAGGCGG
GGCAGGCTGG
CCCGATCCTA
GTCATGCGCT
ACCGCAACCT
GATCGAGGGC
GAAGCATCCG
CCGGTTCCTA
ATGTACGGAG
CAGATGCTAG
GGCAAATTGC
CCTAGCAGGG
GAAAAAGGTC
GAAAAGGTCT
CTTTCCTGTG
GATAGCACGT
ACATTGGGAA
CCCAAAGCCG
TACATTGGGA
ACCGGAACCC
GTACATTGGG
AACCCAAAGC
CGTACATTGG
GAACCGGTCA
CACATGTAAG
TGACTGATAT
AAAAGAGAAA
AAAGGCGATT
TTTCCGCCTA
AAACTCTTTA
AAACTTATTA
AAACTCTTAA
AACCCGCCTG
GCCTGTGCAT
AACTGTCTGG
CCAGCGCACA
GCCGAAGAGC
TGCAAAAAGC
GCCTACCCTT
CGGTCGCTGC
GCTCCCTACG
CCCCGCCGCT
TCGCGTCGGC
CTATCGCGGC
CGCTGGCCGC
TCAAAAATGG
CTGGCCTACG
GCCAGGCAAT
CTACCAGGGC
GCGGACAAGC
CGCGCCGTCG
CCACTCGACC
GCCGGCGCCC
ACATCAAGGC
ACCCTGCCTC
GCGCGTTTCG
GTGATGACGG
TGAAAACCTC
TGACACATGC
AGCTCCCGGA
GACGGTCACA
GCTTGTCTGT
AAGCGGATGC
CGGGAGCAGA
CAAGCCCGTC
AGGGCGCGTC
AGCGGGTGTT
GGCGGGTGTC
GGGGCGCAGC
CATGACCCAG
TCACGTAGCG
ATAGCGGAGT
GTATACTGGC
TTAACTATGC
GGCATCAGAG
CAGATTGTAC
TGAGAGTGCA
CCATATGCGG
TGTGAAATAC
CGCACAGATG
CGTAAGGAGA
AAATACCGCA
TCAGGCGCTC
TTCCGCTTCC
TCGCTCACTG
ACTCGCTGCG
CTCGGTCGTT
CGGCTGCGGC
GAGCGGTATC
AGCTCACTCA
AAGGCGGTAA
TACGGTTATC
CACAGAATCA
GGGGATAACG
CAGGAAAGAA
CATGTGAGCA
AAAGGCCAGC
AAAAGGCCAG
GAACCGTAAA
AAGGCCGCGT
TGCTGGCGTT
TTTCCATAGG
CTCCGCCCCC
CTGACGAGCA
TCACAAAAAT
CGACGCTCAA
GTCAGAGGTG
GCGAAACCCG
ACAGGACTAT
AAAGATACCA
GGCGTTTCCC
CCTGGAAGCT
CCCTCGTGCG
CTCTCCTGTT
CCGACCCTGC
CGCTTACCGG
ATACCTGTCC
GCCTTTCTCC
CTTCGGGAAG
CGTGGCGCTT
TCTCATAGCT
CACGCTGTAG
GTATCTCAGT
TCGGTGTAGG
TCGTTCGCTC
CAAGCTGGGC
TGTGTGCACG
AACCCCCCGT
TCAGCCCGAC
CGCTGCGCCT
TATCCGGTAA
CTATCGTCTT
GAGTCCAACC
CGGTAAGACA
CGACTTATCG
CCACTGGCAG
CAGCCACTGG
TAACAGGATT
AGCAGAGCGA
GGTATGTAGG
CGGTGCTACA
GAGTTCTTGA
AGTGGTGGCC
TAACTACGGC
TACACTAGAA
GGACAGTATT
TGGTATCTGC
GCTCTGCTGA
AGCCAGTTAC
CTTCGGAAAA
AGAGTTGGTA
GCTCTTGATC
CGGCAAACAA
ACCACCGCTG
GTAGCGGTGG
TTTTTTTGTT
TGCAAGCAGC
AGATTACGCG
CAGAAAAAAA
GGATCTCAAG
AAGATCCTTT
GATCTTTTCT
ACGGGGTCTG
ACGCTCAGTG
GAACGAAAAC
TCACGTTAAG
GGATTTTGGT
CATGCATTCT
AGGTACTAAA
ACAATTCATC
CAGTAAAATA
TAATATTTTA
TTTTCTCCCA
ATCAGGCTTG
ATCCCCAGTA
AGTCAAAAAA
TAGCTCGACA
TACTGTTCTT
CCCCGATATC
CTCCCTGATC
GACCGGACGC
AGAAGGCAAT
GTCATACCAC
TTGTCCGCCC
TGCCGCTTCT
CCCAAGATCA
ATAAAGCCAC
TTACTTTGCC
ATCTTTCACA
AAGATGTTGC
TGTCTCCCAG
GTCGCCGTGG
GAAAAGACAA
GTTCCTCTTC
GGGCTTTTCC
GTCTTTAAAA
AATCATACAG
CTCGCGCGGA
TCTTTAAATG
GAGTGTCTTC
TTCCCAGTTT
TCGCAATCCA
CATCGGCCAG
ATCGTTATTC
AGTAAGTAAT
CCAATTCGGC
TAAGCGGCTG
TCTAAGCTAT
TCGTATAGGG
ACAATCCGAT
ATGTCGATGG
AGTGAAAGAG
CCTGATGCAC
TCCGCATACA
GCTCGATAAT
CTTTTCAGGG
CTTTGTTCAT
CTTCATACTC
TTCCGAGCAA
AGGACGCCAT
CGGCCTCACT
CATGAGCAGA
TTGCTCCAGC
CATCATGCCG
TTCAAAGTGC
AGGACCTTTG
GAACAGGCAG
CTTTCCTTCC
AGCCATAGCA
TCATGTCCTT
TTCCCGTTCC
ACATCATAGG
TGGTCCCTTT
ATACCGGCTG
TCCGTCATTT
TTAAATATAG
GTTTTCATTT
TCTCCCACCA
GCTTATATAC
CTTAGCAGGA
GACATTCCTT
CCGTATCTTT
TACGCAGCGG
TATTTTTCGA
TCAGTTTTTT
CAATTCCGGT
GATATTCTCA
TTTTAGCCAT
TTATTATTTC
CTTCCTCTTT
TCTACAGTAT
TTAAAGATAC
CCCAAGAAGC
TAATTATAAC
AAGACGAACT
CCAATTCACT
GTTCCTTGCA
TTCTAAAACC
TTAAATACCA
GAAAACAGCT
TTTTCAAAGT
TGTTTTCAAA
GTTGGCGTAT
AACATAGTAT
CGACGGAGCC
GATTTTGAAA
CCGCGGTGAT
CACAGGCAGC
AACGCTCTGT
CATCGTTACA
ATCAACATGC
TACCCTCCGC
GAGATCATCC
GTGTTTCAAA
CCCGGCAGCT
TAGTTGCCGT
TCTTCCGAAT
AGCATCGGTA
ACATGAGCAA
AGTCTGCCGC
CTTACAACGG
CTCTCCCGCT
GACGCCGTCC
CGGACTGATG
GGCTGCCTGT
ATCGAGTGGT
GATTTTGTGC
CGAGCTGCCG
GTCGGGGAGC
TGTTGGCTGG
CTGGTGGCAG
GATATATTGT
GGTGTAAACA
AATTGACGCT
TAGACAACTT
AATAACACAT
TGCGGACGTT
TTTAATGTAC
TGAATTAACG
CCGAATTAAT
TCGGGGGATC
TGGATTTTAG
TACTGGATTT
TGGTTTTAGG
AATTAGAAAT
TTTATTGATA
GAAGTATTTT
ACAAATACAA
ATACATACTA
AGGGTTTCTT
ATATGCTCAA
CACATGAGCG
AAACCCTATA
GGAACCCTAA
TTCCCTTATC
TGGGAACTAC
TCACACATTA
TTATGGAGAA
ACTCGAGGCA
TTGCAGATGA
GCTGTATCTG
GAAGAGGTAA
ACCCGAAACG
CGTTTTATTC
TTGTTGACAT
GGAGCTATTA
AATCACTAGA
AGGCACTCTT
TGCTGCTTGG
ACAAATGAAC
GTATCTTATC
GAGATCCTGA
ACACCATTTG
TCTCAACTCC
GGAGCTGACA
TCGACACCAA
CGATCTTATA
TCCAGATTCG
TCAAGCTGTT
TGATGATTTC
AGTAACGTTA
AGTGGATCAT
GATAATCATC
GCAAGACCGG
CAACAGGATT
CAATCTTAAG
AAACTTTATT
GCCAAATGTT
TGAACGATCT
GCAGGTCGAC
GGACTATTTC
TTTGCCCTCG
GACGAGTGCT
GGGGCGTCGG
TTTCCACTAT
CGGCGAGTAC
TTCTACACAG
CCATCGGTCC
AGACGGCCGC
GCTTCTGCGG
GCGATTTGTG
TACGCCCGAC
AGTCCCGGCT
CCGGATCGGA
CGATTGCGTC
GCATCGACCC
TGCGCCCAAG
CTGCATCATC
GAAATTGCCG
TCAACCAAGC
TCTGATAGAG
TTGGTCAAGA
CCAATGCGGA
GCATATACGC
CCGGAGTCGT
GGCGATCCTG
CAAGCTCCGG
ATGCCTCCGC
TCGAAGTAGC
GCGTCTGCTG
CTCCATACAA
GCCAACCACG
GCCTCCAGAA
GAAGATGTTG
GCGACCTCGT
ATTGGGAATC
CCCGAACATC
GCCTCGCTCC
AGTCAATGAC
CGCTGTTATG
CGGCCATTGT
CCGTCAGGAC
ATTGTTGGAG
CCGAAATCCG
CGTGCACGAG
GTGCCGGACT
TCGGGGCAGT
CCTCGGCCCA
AAGCATCAGC
TCATCGAGAG
CCTGCGCGAC
GGACGCACTG
ACGGTGTCGT
CCATCACAGT
TTGCCAGTGA
TACACATGGG
GATCAGCAAT
CGCGCATATG
AAATCACGCC
ATGTAGTGTA
TTGACCGATT
CCTTGCGGTC
CGAATGGGCC
GAACCCGCTC
GTCTGGCTAA
GATCGGCCGC
AGCGATCGCA
TCCATAGCCT
CCGCGACCGG
TTGTAGAACA
GCGGGCAGTT
CGGTTTCAGG
CAGGTCTTGC
AACGTGACAC
CCTGTGCACG
GCGGGAGATG
CAATAGGTCA
GGCTCTCGCT
AAACTCCCCA
ATGTCAAGCA
CTTCCGGAAT
CGGGAGCGCG
GCCGATGCAA
AGTGCCGATA
AACATAACGA
TCTTTGTAGA
AACCATCGGC
GCAGCTATTT
ACCCGCAGGA
CATATCCACG
CCCTCCTACA
TCGAAGCTGA
AAGCACGAGA
TTCTTCGCCC
TCCGAGAGCT
GCATCAGGTC
GGAGACGCTG
TCGAACTTTT
CGATCAGAAA
CTTCTCGACA
GACGTCGCGG
TGAGTTCAGG
CTTTTTCATG
GTTACTTCCT
AATCGAAGCT
TTGCTTGGGT
AGAATAGGTA
AGTCAGATTG
AATCTGAAAT
AAAGGGAGGA
AGGGCGAACT
TAAGAAGGTA
TGACCGGGTC
GTTCACTTAC
CTTGCTTGAC
AAACGCACCA
AGTTATCGTG
CACCAAGCAG
CAGATGATAA
TAATGTCCTC
GTTCCTGTCT
GCTAATAAGA
GTCACACTTC
GAGCGCCGCC
GCTACTGCTA
CAAGTGGGGC
TGATCTGACC
AGTTGCCTAA
ATGAACCATC
TTGTCAAACG
ACACAAATTT
TGTGCTCACC
GCCTGGACGA
CTAAACCAAA
ATAGGCATTC
ATTGTTGACC
TCCACTAGCT
CCAGCCAAGC
CCAAAAAGTG
CTCCTTCAAT
ATCATCTTCT
GTCGAGTCTA
GAGCACATTC
TGCGGCCTCT
GTGCTCTGCA
AGCCGCAAAC
TTTCACCAAT
GGACCAGAAC
TACCTGTGAA
ATTAATAACA
GACATACTCC
AAGCTGCCTT
TGTGTGCTTA
ATCACGTATA
CTCACGTGCT
CAATAGTCAC
CAATGCCCTC
CCTCTTGGCC
CTCTCCTTTT
CTTTTTTCGA
CCGAATTGAG
CTCGGTACCA
AACGTTGCTA
GCAGTTCCAG
GTGGAATGTT
ATGATGAGCA
TTGTATTAAA
TCAGGAGATA
TAGCATGATC
TCTAGTTAGC
TCACCACAAA
AGTCAGACGG
CGTAACCAAA
AGTCACACAA
CACAAGCTGT
AAGGATTTCG
GCACGGCTAC
GGAAGACGGA
GAAGCCACCT
TCAGTGGACT
CGAGTACCAT
TTAATTCTAT
TTGTGTTTGA
TCGAGACCTA
ATACAGCCCC
TACAACGACC
ATCAAAGTCG
TATAGCTACC
AGTGAGGAAG
TGGACTCAAA
TCGACTTCAG
CAACATCTCC
TGGATAAACT
TTAAGCCTAA
ACTATACAGA
ATAAGATAGG
TGGAGAGCTT
ATACCGAGCT
CCCAAATCTG
TCCAGATCAT
GGTTGACCGG
TGCCTGGATC
TTCCTATAGA
ATCATCCTTA
TTCGTTGACC
TAGCTGATTC
TGGAGTGACC
CAGAGGGTCA
TGACTTGAGC
CTAAAATCCG
CCGCCTCCAC
CATTTGTAGA
AAAATGTGAC
GAACTCGTGA
GCTCTGTACA
GTGACCGGTG
ACTCTTTCTG
GCATGCGGAG
AGACGGACGG
ACGCAGAGAG
AAGGGCTGAG
TAATAAGCCA
CTGGCCAGAC
AGCTCTGGCG
GCTCTGAGGT
GCAGTGGATG
ATTATTAATC
CGGGACCGGC
CGCCCCTCCG
CCCCGAAGTG
GAAAGGCTGG
TGTGCCCCTC
GTTGACCAAG
AATCTATTGC
ATCATCGGAG
AATATGGAGC
TTCATCGAAT
CACCGGCAGT
AAGCGAAGGA
GAATGTGAAG
CCAGGGGTGT
ATAGCCGTCG
GCGAAATAGC
ATGCCATTAA
CCTAGGTACA
GAAGTCCAAT
TGCTTCCGAT
CTGGTAAAAG
ATTCACGAGA
TAGTACCTTC
TCCGAAGTAG
GTAGAGCGAG
TACCCGGCGC
GTAAGCTCCC
TAATTGGCCC
ATCCGGCATC
TGTAGGGCGT
CCAAATATCG
TGCCTCTCCT
GCTTTGCCCG
GTGTATGAAA
CCGGAAAGGC
CGCTCAGGAG
CTGGCCAGCG
GCGCAGACCG
GGAACACAAG
CTGGCAGTCG
ACCCATCCGG
TGCTCTGCAC
TCGACCTGCT
GAGGTCCCTC
AGTCCCTGGT
AGGCAGCTTT
GCCCCGTCTG
TCCGCCCGGT
GTGTCGGCGG
GGTTGACAAG
GTCGTTGCGT
CAGTCCAACA
TTTGTTGCCA
TATTTTCCTG
CTCTCCCCAC
CAGCTGCTCT
TTTCTTTTCT
CTTTCTTTTC
CCATCTTCAG
TATATTCATC
TTCCCATCCA
AGAACCTTTA
TTTCCCCTAA
GTAAGTACTT
TGCTACATCC
ATACTCCATC
CTTCCCATCC
CTTATTCCTT
TGAACCTTTC
AGTTCGAGCT
TTCCCACTTC
ATCGCAGCTT
GACTAACAGC
TACCCCGCTT
GAGCAGACAT
CACCATGGT