名称: | pMBP-LbCas12a |
---|---|
别称: | Plasmid#113431 |
基因长: | |
质粒宿主: | 大肠杆菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | T7 |
复制子: | pBR322 |
拷贝数: | 低 |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CCAGGACCCA
ACGCTGCCCG
AGATGCGCCG
CGTGCGGCTG
CTGGAGATGG
CGGACGCGAT
GGATATGTTC
TGCCAAGGGT
TGGTTTGCGC
ATTCACAGTT
CTCCGCAAGA
ATTGATTGGC
TCCAATTCTT
GGAGTGGTGA
ATCCGTTAGC
GAGGTGCCGC
CGGCTTCCAT
TCAGGTCGAG
GTGGCCCGGC
TCCATGCACC
GCGACGCAAC
GCGGGGAGGC
AGACAAGGTA
TAGGGCGGCG
CCTACAATCC
ATGCCAACCC
GTTCCATGTG
CTCGCCGAGG
CGGCATAAAT
CGCCGTGACG
ATCAGCGGTC
CAGTGATCGA
AGTTAGGCTG
GTAAGAGCCG
CGAGCGATCC
TTGAAGCTGT
CCCTGATGGT
CGTCATCTAC
CTGCCTGGAC
AGCATGGCCT
GCAACGCGGG
CATCCCGATG
CCGCCGGAAG
CGAGAAGAAT
CATAATGGGG
AAGGCCATCC
AGCCTCGCGT
CGCGAACGCC
AGCAAGACGT
AGCCCAGCGC
GTCGGCCGCC
ATGCCGGCGA
TAATGGCCTG
CTTCTCGCCG
AAACGTTTGG
TGGCGGGACC
AGTGACGAAG
GCTTGAGCGA
GGGCGTGCAA
GATTCCGAAT
ACCGCAAGCG
ACAGGCCGAT
CATCGTCGCG
CTCCAGCGAA
AGCGGTCCTC
GCCGAAAATG
ACCCAGAGCG
CTGCCGGCAC
CTGTCCTACG
AGTTGCATGA
TAAAGAAGAC
AGTCATAAGT
GCGGCGACGA
TAGTCATGCC
CCGCGCCCAC
CGGAAGGAGC
TGACTGGGTT
GAAGGCTCTC
AAGGGCATCG
GTCGACGCTC
TCCCTTATGC
GACTCCTGCA
TTAGGAAGCA
GCCCAGTAGT
AGGTTGAGGC
CGTTGAGCAC
CGCCGCCGCA
AGGAATGGTG
CATGCAAGGA
GATGGCGCCC
AACAGTCCCC
CGGCCACGGG
GCCTGCCACC
ATACCCACGC
CGAAACAAGC
GCTCATGAGC
CCGAAGTGGC
GAGCCCGATC
TTCCCCATCG
GTGATGTCGG
CGATATAGGC
GCCAGCAACC
GCACCTGTGG
CGCCGGTGAT
GCCGGCCACG
ATGCGTCCGG
CGTAGAGGAT
CGAGATCTCG
ATCCCGCGAA
ATTAATACGA
CTCACTATAG
GGAGACCACA
ACGGTTTCCC
TCTAGTGCCG
GCTCCGGAGA
GCTCTTTAAT
TAAGCGGCCG
CCCTGCAGGA
CTCGAGTTCT
AGAAATAATT
TTGTTTAACT
TTAAGAAGGA
GATATACATA
TGAAATCTTC
TCACCATCAC
CATCACCATC
ACCATCACCA
TGGTTCTTCT
ATGAAAATCG
AAGAAGGTAA
ACTGGTAATC
TGGATTAACG
GCGATAAAGG
CTATAACGGT
CTCGCTGAAG
TCGGTAAGAA
ATTCGAGAAA
GATACCGGAA
TTAAAGTCAC
CGTTGAGCAT
CCGGATAAAC
TGGAAGAGAA
ATTCCCACAG
GTTGCGGCAA
CTGGCGATGG
CCCTGACATT
ATCTTCTGGG
CACACGACCG
CTTTGGTGGC
TACGCTCAAT
CTGGCCTGTT
GGCTGAAATC
ACCCCGGACA
AAGCGTTCCA
GGACAAGCTG
TATCCGTTTA
CCTGGGATGC
CGTACGTTAC
AACGGCAAGC
TGATTGCTTA
CCCGATCGCT
GTTGAAGCGT
TATCGCTGAT
TTATAACAAA
GATCTGCTGC
CGAACCCGCC
AAAAACCTGG
GAAGAGATCC
CGGCGCTGGA
TAAAGAACTG
AAAGCGAAAG
GTAAGAGCGC
GCTGATGTTC
AACCTGCAAG
AACCGTACTT
CACCTGGCCG
CTGATTGCTG
CTGACGGGGG
TTATGCGTTC
AAGTATGAAA
ACGGCAAGTA
CGACATTAAA
GACGTGGGCG
TGGATAACGC
TGGCGCGAAA
GCGGGTCTGA
CCTTCCTGGT
TGACCTGATT
AAAAACAAAC
ACATGAATGC
AGACACCGAT
TACTCCATCG
CAGAAGCTGC
CTTTAATAAA
GGCGAAACAG
CGATGACCAT
CAACGGCCCG
TGGGCATGGT
CCAACATCGA
CACCAGCAAA
GTGAATTATG
GTGTAACGGT
ACTGCCGACC
TTCAAGGGTC
AACCATCCAA
ACCGTTCGTT
GGCGTGCTGA
GCGCAGGTAT
TAACGCCGCC
AGTCCGAACA
AAGAGCTGGC
AAAAGAGTTC
CTCGAAAACT
ATCTGCTGAC
TGATGAAGGT
CTGGAAGCGG
TTAATAAAGA
CAAACCGCTG
GGTGCCGTAG
CGCTGAAGTC
TTACGAGGAA
GAGTTGGCGA
AAGATCCACG
TATTGCCGCC
ACTATGGAAA
ACGCCCAGAA
AGGTGAAATC
ATGCCGAACA
TCCCGCAGAT
GTCCGCTTTC
TGGTATGCCG
TGCGTACTGC
GGTGATCAAC
GCCGCCAGCG
GTCGTCAGAC
TGTCGATGAA
GCCCTGAAAG
ACGCGCAGAC
TAATTCGAGC
TCGAACAACA
ACAACAATAA
CAATAACAAC
AACCTCGGGA
TCGAGGAAAA
CCTGTACTTC
CAATCCAATG
CAATGAGCAA
ACTGGAGAAG
TTCACGAACT
GTTATTCTCT
GTCGAAAACC
TTACGTTTTA
AGGCCATTCC
GGTAGGTAAA
ACGCAAGAAA
ATATCGACAA
TAAACGTCTC
CTGGTTGAAG
ATGAAAAGCG
TGCCGAAGAT
TATAAAGGTG
TTAAAAAGCT
GCTTGATCGC
TATTACCTGT
CTTTTATCAA
CGATGTTCTG
CATTCCATCA
AATTGAAAAA
CTTGAATAAT
TACATCTCGT
TATTTCGCAA
AAAAACCCGT
ACCGAAAAAG
AAAACAAGGA
GCTGGAAAAC
CTTGAAATTA
ATCTGCGTAA
AGAAATCGCG
AAAGCATTTA
AAGGTAATGA
AGGATATAAA
TCTCTGTTTA
AAAAAGACAT
TATTGAAACC
ATCCTGCCTG
AGTTCTTGGA
TGATAAAGAT
GAAATTGCCC
TGGTTAATAG
CTTTAATGGC
TTTACGACTG
CATTTACCGG
CTTTTTTGAT
AACCGTGAAA
ACATGTTCAG
CGAAGAAGCG
AAATCGACTT
CCATTGCCTT
CCGTTGCATT
AATGAAAACC
TGACCCGCTA
CATTTCTAAT
ATGGACATCT
TTGAAAAAGT
TGATGCCATT
TTCGACAAAC
ATGAAGTTCA
AGAAATCAAA
GAAAAGATTT
TAAACTCCGA
TTATGATGTT
GAAGATTTCT
TTGAGGGAGA
ATTTTTCAAT
TTTGTTCTCA
CCCAAGAGGG
TATTGATGTC
TATAACGCCA
TTATCGGAGG
ATTTGTTACG
GAATCAGGCG
AAAAGATTAA
AGGACTGAAT
GAATACATTA
ATTTATACAA
TCAGAAAACC
AAACAGAAAT
TGCCAAAATT
CAAACCACTG
TATAAACAGG
TATTAAGCGA
CCGTGAATCC
TTGTCCTTTT
ATGGCGAAGG
CTATACAAGC
GATGAAGAGG
TGCTCGAAGT
ATTCCGTAAT
ACTTTGAACA
AAAACTCGGA
AATCTTCTCC
AGCATTAAAA
AACTGGAAAA
ATTGTTTAAA
AATTTCGATG
AATATAGTAG
CGCAGGCATC
TTCGTCAAAA
ATGGCCCTGC
GATTTCAACC
ATTTCTAAAG
ACATTTTCGG
TGAATGGAAC
GTGATCCGCG
ATAAATGGAA
CGCGGAGTAT
GACGACATTC
ATCTGAAAAA
AAAAGCCGTT
GTTACCGAAA
AGTATGAAGA
TGACCGCCGC
AAGAGCTTTA
AAAAAATTGG
TAGTTTCTCA
CTGGAACAGT
TACAAGAATA
TGCAGACGCC
GATCTGTCGG
TTGTGGAAAA
GTTAAAAGAG
ATTATTATCC
AGAAAGTCGA
TGAAATTTAT
AAAGTGTACG
GCTCCAGCGA
AAAGCTCTTT
GATGCCGACT
TTGTTTTAGA
AAAATCGCTG
AAAAAAAACG
ATGCCGTGGT
GGCCATCATG
AAAGATCTGT
TGGATTCGGT
CAAATCGTTC
GAAAACTATA
TCAAGGCGTT
CTTTGGCGAA
GGGAAAGAGA
CCAATCGTGA
CGAATCGTTC
TATGGCGATT
TTGTGTTGGC
GTATGATATT
CTTCTGAAAG
TAGATCACAT
CTATGATGCT
ATCCGCAACT
ACGTGACCCA
GAAACCGTAT
TCGAAGGATA
AATTTAAGCT
GTATTTTCAA
AACCCTCAGT
TTATGGGTGG
CTGGGATAAA
GATAAAGAAA
CCGACTACCG
CGCGACGATC
TTGCGCTATG
GCTCTAAGTA
CTATTTAGCC
ATCATGGATA
AAAAGTATGC
AAAATGCCTC
CAGAAAATCG
ATAAGGATGA
TGTCAACGGG
AACTACGAAA
AAATCAACTA
CAAACTCTTG
CCTGGCCCGA
ATAAAATGCT
GCCGAAAGTC
TTTTTTTCCA
AAAAATGGAT
GGCCTATTAT
AACCCTTCCG
AAGATATTCA
GAAGATTTAC
AAGAACGGTA
CCTTTAAAAA
AGGTGACATG
TTTAATCTGA
ATGACTGTCA
CAAGCTTATT
GATTTCTTCA
AAGATAGTAT
TTCTCGCTAC
CCTAAATGGT
CAAACGCGTA
CGATTTTAAT
TTCTCAGAAA
CAGAGAAATA
CAAAGATATT
GCGGGGTTTT
ATCGCGAAGT
CGAAGAACAA
GGGTATAAGG
TGTCGTTCGA
ATCGGCTTCC
AAAAAGGAAG
TGGATAAACT
TGTTGAAGAA
GGCAAACTCT
ATATGTTCCA
GATCTACAAC
AAAGATTTCT
CCGACAAGAG
CCATGGCACG
CCGAACTTAC
ATACCATGTA
CTTTAAATTA
CTGTTCGATG
AAAATAATCA
TGGTCAGATT
CGCTTGAGCG
GTGGCGCCGA
ACTGTTTATG
CGCCGCGCAT
CATTGAAAAA
GGAAGAACTT
GTTGTGCACC
CAGCAAACAG
TCCGATTGCA
AACAAAAATC
CGGATAACCC
GAAGAAGACT
ACCACCTTAA
GCTATGATGT
ATATAAAGAT
AAACGTTTTA
GCGAAGATCA
GTATGAACTC
CACATTCCGA
TCGCGATTAA
CAAATGTCCT
AAGAACATCT
TTAAAATCAA
TACCGAGGTT
CGTGTACTGC
TCAAACACGA
CGACAACCCC
TATGTTATTG
GCATTGACCG
CGGCGAACGC
AACCTGCTGT
ATATTGTCGT
TGTGGATGGC
AAAGGTAACA
TTGTTGAACA
GTATAGCCTT
AATGAAATCA
TCAATAATTT
CAACGGCATT
CGTATTAAAA
CGGACTATCA
TAGCCTGCTG
GACAAAAAAG
AGAAAGAACG
CTTCGAAGCC
CGTCAGAATT
GGACCTCTAT
CGAAAATATC
AAGGAACTGA
AAGCTGGGTA
CATTTCTCAA
GTGGTCCACA
AGATTTGCGA
ACTGGTGGAA
AAGTATGACG
CGGTGATCGC
ACTGGAGGAT
CTGAATTCGG
GCTTTAAAAA
TAGCCGTGTG
AAGGTGGAGA
AACAGGTTTA
CCAAAAGTTC
GAAAAAATGC
TGATTGACAA
ACTTAATTAC
ATGGTGGACA
AAAAATCTAA
TCCATGTGCG
ACCGGCGGGG
CTCTCAAAGG
CTATCAGATT
ACGAATAAAT
TCGAAAGCTT
CAAATCCATG
TCTACCCAGA
ACGGATTTAT
CTTTTACATC
CCCGCCTGGC
TGACTTCCAA
AATTGATCCG
AGTACCGGTT
TTGTTAATCT
GCTGAAAACC
AAATATACGT
CGATCGCCGA
TTCAAAAAAA
TTCATTAGCA
GCTTCGACCG
TATTATGTAC
GTCCCGGAAG
AAGACCTGTT
CGAATTCGCC
CTTGACTATA
AAAATTTCTC
GCGTACGGAT
GCCGATTATA
TCAAAAAATG
GAAACTGTAC
AGCTACGGCA
ACCGCATCCG
CATTTTTCGC
AACCCAAAGA
AGAACAACGT
ATTTGACTGG
GAGGAAGTTT
GCTTGACGAG
CGCTTACAAA
GAGCTCTTTA
ACAAATATGG
GATCAATTAT
CAGCAAGGGG
ACATCCGTGC
GTTGCTGTGT
GAGCAGTCAG
ATAAAGCGTT
CTACAGCTCT
TTCATGGCTT
TAATGTCGCT
GATGTTGCAG
ATGCGCAATT
CGATCACTGG
CCGCACCGAT
GTGGATTTCC
TGATTAGCCC
GGTCAAAAAT
AGCGATGGCA
TTTTTTACGA
TTCACGTAAC
TATGAAGCCC
AGGAAAACGC
TATTCTGCCG
AAGAACGCTG
ATGCGAACGG
CGCGTACAAT
ATTGCCCGTA
AGGTACTGTG
GGCAATTGGT
CAGTTCAAGA
AAGCGGAAGA
TGAAAAGTTG
GATAAGGTCA
AAATTGCGAT
CTCGAATAAA
GAATGGCTGG
AATATGCGCA
GACCAGCGTG
AAACATTAAT
AACATTGGAA
GTGGATAACG
GATCCGCGAT
CGCGGCGCGC
CACCTGGTGG
CCGGCCGGTA
CCACGCGTGC
GCGCTGATCC
GGCTGCTAAC
AAAGCCCGAA
AGGAAGCTGA
GTTGGCTGCT
GCCACCGCTG
AGCAATAACT
AGCATAACCC
CTTGGGGCCT
CTAAACGGGT
CTTGAGGGGT
TTTTTGCTGA
AAGGAGGAAC
TATATCCGGA
TATCCACAGG
ACGGGTGTGG
TCGCCATGAT
CGCGTAGTCG
ATAGTGGCTC
CAAGTAGCGA
AGCGAGCAGG
ACTGGGCGGC
GGCCAAAGCG
GTCGGACAGT
GCTCCGAGAA
CGGGTGCGCA
TAGAAATTGC
ATCAACGCAT
ATAGCGCTAG
CAGCACGCCA
TAGTGACTGG
CGATGCTGTC
GGAATGGACG
ATATCCCGCA
AGAGGCCCGG
CAGTACCGGC
ATAACCAAGC
CTATGCCTAC
AGCATCCAGG
GTGACGGTGC
CGAGGATGAC
GATGAGCGCA
TTGTTAGATT
TCATACACGG
TGCCTGACTG
CGTTAGCAAT
TTAACTGTGA
TAAACTACCG
CATTAAAGCT
TATCGATGAT
AAGCTGTCAA
ACATGAGAAT
TCTTGAAGAC
GAAAGGGCCT
CGTGATACGC
CTATTTTTAT
AGGTTAATGT
CATGATAATA
ATGGTTTCTT
AGACGTCAGG
TGGCACTTTT
CGGGGAAATG
TGCGCGGAAC
CCCTATTTGT
TTATTTTTCT
AAATACATTC
AAATATGTAT
CCGCTCATGA
GACAATAACC
CTGATAAATG
CTTCAATAAC
ATTGAAAAAG
GAAGAGTATG
AGTATTCAAC
ATTTCCGTGT
CGCCCTTATT
CCCTTTTTTG
CGGCATTTTG
CCTTCCTGTT
TTTGCTCACC
CAGAAACGCT
GGTGAAAGTA
AAAGATGCTG
AAGATCAGTT
GGGTGCACGA
GTGGGTTACA
TCGAACTGGA
TCTCAACAGC
GGTAAGATCC
TTGAGAGTTT
TCGCCCCGAA
GAACGTTTTC
CAATGATGAG
CACTTTTAAA
GTTCTGCTAT
GTGGCGCGGT
ATTATCCCGT
GTTGACGCCG
GGCAAGAGCA
ACTCGGTCGC
CGCATACACT
ATTCTCAGAA
TGACTTGGTT
GAGTACTCAC
CAGTCACAGA
AAAGCATCTT
ACGGATGGCA
TGACAGTAAG
AGAATTATGC
AGTGCTGCCA
TAACCATGAG
TGATAACACT
GCGGCCAACT
TACTTCTGAC
AACGATCGGA
GGACCGAAGG
AGCTAACCGC
TTTTTTGCAC
AACATGGGGG
ATCATGTAAC
TCGCCTTGAT
CGTTGGGAAC
CGGAGCTGAA
TGAAGCCATA
CCAAACGACG
AGCGTGACAC
CACGATGCCT
GCAGCAATGG
CAACAACGTT
GCGCAAACTA
TTAACTGGCG
AACTACTTAC
TCTAGCTTCC
CGGCAACAAT
TAATAGACTG
GATGGAGGCG
GATAAAGTTG
CAGGACCACT
TCTGCGCTCG
GCCCTTCCGG
CTGGCTGGTT
TATTGCTGAT
AAATCTGGAG
CCGGTGAGCG
TGGGTCTCGC
GGTATCATTG
CAGCACTGGG
GCCAGATGGT
AAGCCCTCCC
GTATCGTAGT
TATCTACACG
ACGGGGAGTC
AGGCAACTAT
GGATGAACGA
AATAGACAGA
TCGCTGAGAT
AGGTGCCTCA
CTGATTAAGC
ATTGGTAACT
GTCAGACCAA
GTTTACTCAT
ATATACTTTA
GATTGATTTA
AAACTTCATT
TTTAATTTAA
AAGGATCTAG
GTGAAGATCC
TTTTTGATAA
TCTCATGACC
AAAATCCCTT
AACGTGAGTT
TTCGTTCCAC
TGAGCGTCAG
ACCCCGTAGA
AAAGATCAAA
GGATCTTCTT
GAGATCCTTT
TTTTCTGCGC
GTAATCTGCT
GCTTGCAAAC
AAAAAAACCA
CCGCTACCAG
CGGTGGTTTG
TTTGCCGGAT
CAAGAGCTAC
CAACTCTTTT
TCCGAAGGTA
ACTGGCTTCA
GCAGAGCGCA
GATACCAAAT
ACTGTCCTTC
TAGTGTAGCC
GTAGTTAGGC
CACCACTTCA
AGAACTCTGT
AGCACCGCCT
ACATACCTCG
CTCTGCTAAT
CCTGTTACCA
GTGGCTGCTG
CCAGTGGCGA
TAAGTCGTGT
CTTACCGGGT
TGGACTCAAG
ACGATAGTTA
CCGGATAAGG
CGCAGCGGTC
GGGCTGAACG
GGGGGTTCGT
GCACACAGCC
CAGCTTGGAG
CGAACGACCT
ACACCGAACT
GAGATACCTA
CAGCGTGAGC
TATGAGAAAG
CGCCACGCTT
CCCGAAGGGA
GAAAGGCGGA
CAGGTATCCG
GTAAGCGGCA
GGGTCGGAAC
AGGAGAGCGC
ACGAGGGAGC
TTCCAGGGGG
AAACGCCTGG
TATCTTTATA
GTCCTGTCGG
GTTTCGCCAC
CTCTGACTTG
AGCGTCGATT
TTTGTGATGC
TCGTCAGGGG
GGCGGAGCCT
ATGGAAAAAC
GCCAGCAACG
CGGCCTTTTT
ACGGTTCCTG
GCCTTTTGCT
GGCCTTTTGC
TCACATGTTC
TTTCCTGCGT
TATCCCCTGA
TTCTGTGGAT
AACCGTATTA
CCGCCTTTGA
GTGAGCTGAT
ACCGCTCGCC
GCAGCCGAAC
GACCGAGCGC
AGCGAGTCAG
TGAGCGAGGA
AGCGGAAGAG
CGCCTGATGC
GGTATTTTCT
CCTTACGCAT
CTGTGCGGTA
TTTCACACCG
CATATATGGT
GCACTCTCAG
TACAATCTGC
TCTGATGCCG
CATAGTTAAG
CCAGTATACA
CTCCGCTATC
GCTACGTGAC
TGGGTCATGG
CTGCGCCCCG
ACACCCGCCA
ACACCCGCTG
ACGCGCCCTG
ACGGGCTTGT
CTGCTCCCGG
CATCCGCTTA
CAGACAAGCT
GTGACCGTCT
CCGGGAGCTG
CATGTGTCAG
AGGTTTTCAC
CGTCATCACC
GAAACGCGCG
AGGCAGCTGC
GGTAAAGCTC
ATCAGCGTGG
TCGTGAAGCG
ATTCACAGAT
GTCTGCCTGT
TCATCCGCGT
CCAGCTCGTT
GAGTTTCTCC
AGAAGCGTTA
ATGTCTGGCT
TCTGATAAAG
CGGGCCATGT
TAAGGGCGGT
TTTTTCCTGT
TTGGTCACTG
ATGCCTCCGT
GTAAGGGGGA
TTTCTGTTCA
TGGGGGTAAT
GATACCGATG
AAACGAGAGA
GGATGCTCAC
GATACGGGTT
ACTGATGATG
AACATGCCCG
GTTACTGGAA
CGTTGTGAGG
GTAAACAACT
GGCGGTATGG
ATGCGGCGGG
ACCAGAGAAA
AATCACTCAG
GGTCAATGCC
AGCGCTTCGT
TAATACAGAT
GTAGGTGTTC
CACAGGGTAG
CCAGCAGCAT
CCTGCGATGC
AGATCCGGAA
CATAATGGTG
CAGGGCGCTG
ACTTCCGCGT
TTCCAGACTT
TACGAAACAC
GGAAACCGAA
GACCATTCAT
GTTGTTGCTC
AGGTCGCAGA
CGTTTTGCAG
CAGCAGTCGC
TTCACGTTCG
CTCGCGTATC
GGTGATTCAT
TCTGCTAACC
AGTAAGGCAA
CCCCGCCAGC
CTAGCCGGGT
CCTCAACGAC
AGGAGCACGA
TCATGCGCAC
CCGTGG