名称: | pGIPZ-EnCMV-TurboGFP-IRES-Puro-MEG3(human)-ncRNA-shRNA3-WPRE |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 干扰沉默 |
片段类型: | ncRNA,shRNA |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro,Hyg |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | WPRE-R:CATAGCGTAAAAGGAGCAACA |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGGAAGGGCT
AATTCACTCC
CAAAGAAGAC
AAGATATCCT
TGATCTGTGG
ATCTACCACA
CACAAGGCTA
CTTCCCTGAT
TAGCAGAACT
ACACACCAGG
GCCAGGGGTC
AGATATCCAC
TGACCTTTGG
ATGGTGCTAC
AAGCTAGTAC
CAGTTGAGCC
AGATAAGGTA
GAAGAGGCCA
ATAAAGGAGA
GAACACCAGC
TTGTTACACC
CTGTGAGCCT
GCATGGGATG
GATGACCCGG
AGAGAGAAGT
GTTAGAGTGG
AGGTTTGACA
GCCGCCTAGC
ATTTCATCAC
GTGGCCCGAG
AGCTGCATCC
GGAGTACTTC
AAGAACTGCT
GATATCGAGC
TTGCTACAAG
GGACTTTCCG
CTGGGGACTT
TCCAGGGAGG
CGTGGCCTGG
GCGGGACTGG
GGAGTGGCGA
GCCCTCAGAT
CCTGCATATA
AGCAGCTGCT
TTTTGCCTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACTTGAAAG
CGAAAGGGAA
ACCAGAGGAG
CTCTCTCGAC
GCAGGACTCG
GCTTGCTGAA
GCGCGCACGG
CAAGAGGCGA
GGGGCGGCGA
CTGGTGAGTA
CGCCAAAAAT
TTTGACTAGC
GGAGGCTAGA
AGGAGAGAGA
TGGGTGCGAG
AGCGTCAGTA
TTAAGCGGGG
GAGAATTAGA
TCGCGATGGG
AAAAAATTCG
GTTAAGGCCA
GGGGGAAAGA
AAAAATATAA
ATTAAAACAT
ATAGTATGGG
CAAGCAGGGA
GCTAGAACGA
TTCGCAGTTA
ATCCTGGCCT
GTTAGAAACA
TCAGAAGGCT
GTAGACAAAT
ACTGGGACAG
CTACAACCAT
CCCTTCAGAC
AGGATCAGAA
GAACTTAGAT
CATTATATAA
TACAGTAGCA
ACCCTCTATT
GTGTGCATCA
AAGGATAGAG
ATAAAAGACA
CCAAGGAAGC
TTTAGACAAG
ATAGAGGAAG
AGCAAAACAA
AAGTAAGACC
ACCGCACAGC
AAGCGGCCGG
CCGCTGATCT
TCAGACCTGG
AGGAGGAGAT
ATGAGGGACA
ATTGGAGAAG
TGAATTATAT
AAATATAAAG
TAGTAAAAAT
TGAACCATTA
GGAGTAGCAC
CCACCAAGGC
AAAGAGAAGA
GTGGTGCAGA
GAGAAAAAAG
AGCAGTGGGA
ATAGGAGCTT
TGTTCCTTGG
GTTCTTGGGA
GCAGCAGGAA
GCACTATGGG
CGCAGCGTCA
ATGACGCTGA
CGGTACAGGC
CAGACAATTA
TTGTCTGGTA
TAGTGCAGCA
GCAGAACAAT
TTGCTGAGGG
CTATTGAGGC
GCAACAGCAT
CTGTTGCAAC
TCACAGTCTG
GGGCATCAAG
CAGCTCCAGG
CAAGAATCCT
GGCTGTGGAA
AGATACCTAA
AGGATCAACA
GCTCCTGGGG
ATTTGGGGTT
GCTCTGGAAA
ACTCATTTGC
ACCACTGCTG
TGCCTTGGAA
TGCTAGTTGG
AGTAATAAAT
CTCTGGAACA
GATTTGGAAT
CACACGACCT
GGATGGAGTG
GGACAGAGAA
ATTAACAATT
ACACAAGCTT
AATACACTCC
TTAATTGAAG
AATCGCAAAA
CCAGCAAGAA
AAGAATGAAC
AAGAATTATT
GGAATTAGAT
AAATGGGCAA
GTTTGTGGAA
TTGGTTTAAC
ATAACAAATT
GGCTGTGGTA
TATAAAATTA
TTCATAATGA
TAGTAGGAGG
CTTGGTAGGT
TTAAGAATAG
TTTTTGCTGT
ACTTTCTATA
GTGAATAGAG
TTAGGCAGGG
ATATTCACCA
TTATCGTTTC
AGACCCACCT
CCCAACCCCG
AGGGGACCCG
ACAGGCCCGA
AGGAATAGAA
GAAGAAGGTG
GAGAGAGAGA
CAGAGACAGA
TCCATTCGAT
TAGTGAACGG
ATCGGCACTG
CGTGCGCCAA
TTCTGCAGAC
AAATGGCAGT
ATTCATCCAC
AATTTTAAAA
GAAAAGGGGG
GATTGGGGGG
TACAGTGCAG
GGGAAAGAAT
AGTAGACATA
ATAGCAACAG
ACATACAAAC
TAAAGAATTA
CAAAAACAAA
TTACAAAAAT
TCAAAATTTT
CGGGTTTATT
ACAGGGACAG
CAGAGATCCA
GTTTGGTTAG
TACCGGGCCC
GCTCTAGTCC
GGAATCAGTC
CTGCTCCTCG
GCCACGAAGT
GCACGCAGTT
GCCGGCCGGG
TCGCGCAGGG
CGAACTCCCG
CCCCCACGGC
TGCTCGCCGA
TCTCGGTCAT
GGCCGGCCCG
GAGGCGTCCC
GGAAGTTCGT
GGACACGACC
TCCGACCACT
CGGCGTACAG
CTCGTCCAGG
CCGCGCACCC
ACACCCAGGC
CAGGGTGTTG
TCCGGCACCA
CCTGGTCCTG
GACCGCGCTG
ATGAACAGGG
TCACGTCGTC
CCGGACCACA
CCGGCGAAGT
CGTCCTCCAC
GAAGTCCCGG
GAGAACCCGA
GCCGGTCGGT
CCAGAACTCG
ACCGCTCCGG
CGACGTCGCG
CGCGGTGAGC
ACCGGAACGG
CACTGGTCAA
CTTGGCCATG
GTGGCCCTCC
TATAGTGAGT
CGTATTATAC
TATGCCGATA
TACTATGCCG
ATGATTAATT
GTCAACACGT
GCTGCAGGTC
CGAGGTTCTA
GACGTATTAC
CGCCATGCAT
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCG
TTTAGTGAAC
CGTCAGATCG
CCTGGAGACG
CCATCCACGC
TGTTTTGACC
TCCATAGAAG
ACACCGACTC
TACTAGAGGA
TCTGCCACCA
TGGAGAGCGA
CGAGAGCGGC
CTGCCCGCCA
TGGAGATCGA
GTGCCGCATC
ACCGGCACCC
TGAACGGCGT
GGAGTTCGAG
CTGGTGGGCG
GCGGAGAGGG
CACCCCCGAG
CAGGGCCGCA
TGACCAACAA
GATGAAGAGC
ACCAAAGGCG
CCCTGACCTT
CAGCCCCTAC
CTGCTGAGCC
ACGTGATGGG
CTACGGCTTC
TACCACTTCG
GCACCTACCC
CAGCGGCTAC
GAGAACCCCT
TCCTGCACGC
CATCAACAAC
GGCGGCTACA
CCAACACCCG
CATCGAGAAG
TACGAGGACG
GCGGCGTGCT
GCACGTGAGC
TTCAGCTACC
GCTACGAGGC
CGGCCGCGTG
ATCGGCGACT
TCAAGGTGAT
GGGCACCGGC
TTCCCCGAGG
ACAGCGTGAT
CTTCACCGAC
AAGATCATCC
GCAGCAACGC
CACCGTGGAG
CACCTGCACC
CCATGGGCGA
TAACGATCTG
GATGGCAGCT
TCACCCGCAC
CTTCAGCCTG
CGCGACGGCG
GCTACTACAG
CTCCGTGGTG
GACAGCCACA
TGCACTTCAA
GAGCGCCATC
CACCCCAGCA
TCCTGCAGAA
CGGGGGCCCC
ATGTTCGCCT
TCCGCCGCGT
GGAGGAGGAT
CACAGCAACA
CCGAGCTGGG
CATCGTGGAG
TACCAGCACG
CCTTCAAGAC
CCCGGATGCA
GATGCCGGTG
AAGAATAATG
TACAAGTAGC
GGCCGCAAAT
TCCGCCCCTC
TCCCTCCCCC
CCCCCTAACG
TTACTGGCCG
AAGCCGCTTG
GAATAAGGCC
GGTGTGCGTT
TGTCTATATG
TTATTTTCCA
CCATATTGCC
GTCTTTTGGC
AATGTGAGGG
CCCGGAAACC
TGGCCCTGTC
TTCTTGACGA
GCATTCCTAG
GGGTCTTTCC
CCTCTCGCCA
AAGGAATGCA
AGGTCTGTTG
AATGTCGTGA
AGGAAGCAGT
TCCTCTGGAA
GCTTCTTGAA
GACAAACAAC
GTCTGTAGCG
ACCCTTTGCA
GGCAGCGGAA
CCCCCCACCT
GGCGACAGGT
GCCTCTGCGG
CCAAAAGCCA
CGTGTATAAG
ATACACCTGC
AAAGGCGGCA
CAACCCCAGT
GCCACGTTGT
GAGTTGGATA
GTTGTGGAAA
GAGTCAAATG
GCTCTCCTCA
AGCGTATTCA
ACAAGGGGCT
GAAGGATGCC
CAGAAGGTAC
CCCATTGTAT
GGGATCTGAT
CTGGGGCCTC
GGTGCACATG
CTTTACATGT
GTTTAGTCGA
GGTTAAAAAA
ACGTCTAGGC
CCCCCGAACC
ACGGGGACGT
GGTTTTCCTT
TGAAAAACAC
GATAATACCA
TGGCCACCGA
GTACAAGCCC
ACGGTGCGCC
TCGCCACCCG
CGACGACGTC
CCCCGGGCCG
TACGCACCCT
CGCCGCCGCG
TTCGCCGACT
ACCCCGCCAC
GCGCCACACC
GTCGACCCGG
ACCGCCACAT
CGAGCGGGTC
ACCGAGCTGC
AAGAACTCTT
CCTCACGCGC
GTCGGGCTCG
ACATCGGCAA
GGTGTGGGTC
GCGGACGACG
GCGCCGCGGT
GGCGGTCTGG
ACCACGCCGG
AGAGCGTCGA
AGCGGGGGCG
GTGTTCGCCG
AGATCGGCTC
GCGCATGGCC
GAGTTGAGCG
GTTCCCGGCT
GGCCGCGCAG
CAACAGATGG
AAGGCCTCCT
GGCGCCGCAC
CGGCCCAAGG
AGCCCGCGTG
GTTCCTGGCC
ACCGTCGGCG
TCTCGCCCGA
CCACCAGGGC
AAGGGTCTGG
GCAGCGCCGT
CGTGCTCCCC
GGAGTGGAGG
CGGCCGAGCG
CGCTGGGGTG
CCCGCCTTCC
TGGAGACCTC
CGCGCCCCGC
AACCTCCCCT
TCTACGAGCG
GCTCGGCTTC
ACCGTCACCG
CCGACGTCGA
GGTGCCCGAA
GGACCGCGCA
CCTGGTGCAT
GACCCGCAAG
CCCGGTGCCT
GAGTTTGTTT
GAATGAGGCT
TCAGTACTTT
ACAGAATCGT
TGCCTGCACA
TCTTGGAAAC
ACTTGCTGGG
ATTACTTCTT
CAGGTTAACC
CAACAGAAGG
CTCGAGCAAA
GGTCAGGGCT
TAATGTTCAA
GAGACATTAA
GCCCTGACCT
TTGTTTTTTG
GATCCTGATC
AGAATTCAAG
GGGCTACTTT
AGGAGCAATT
ATCTTGTTTA
CTAAAACTGA
ATACCTTGCT
ATCTCTTTGA
TACATTTTTA
CAAAGCTGAA
TTAAAATGGT
ATAAATTAAA
TCACTTTTTT
CAATTGGAAG
ACTAATGCGG
CCGGCCATTA
CTCCGTCTCG
TGTCTTGTTG
CATATGTCTG
CTGGTTTGTT
TGATGTTGTT
TGCGGGCGGG
CCCTATAGTG
AGTCGTATTA
CCTAGGACGC
GTCTGGAACA
ATCAACCTCT
GGATTACAAA
ATTTGTGAAA
GATTGACTGG
TATTCTTAAC
TATGTTGCTC
CTTTTACGCT
ATGTGGATAC
GCTGCTTTAA
TGCCTTTGTA
TCATGCTATT
GCTTCCCGTA
TGGCTTTCAT
TTTCTCCTCC
TTGTATAAAT
CCTGGTTGCT
GTCTCTTTAT
GAGGAGTTGT
GGCCCGTTGT
CAGGCAACGT
GGCGTGGTGT
GCACTGTGTT
TGCTGACGCA
ACCCCCACTG
GTTGGGGCAT
TGCCACCACC
TGTCAGCTCC
TTTCCGGGAC
TTTCGCTTTC
CCCCTCCCTA
TTGCCACGGC
GGAACTCATC
GCCGCCTGCC
TTGCCCGCTG
CTGGACAGGG
GCTCGGCTGT
TGGGCACTGA
CAATTCCGTG
GTGTTGTCGG
GGAAGCTGAC
GTCCTTTCCA
TGGCTGCTCG
CCTGTGTTGC
CACCTGGATT
CTGCGCGGGA
CGTCCTTCTG
CTACGTCCCT
TCGGCCCTCA
ATCCAGCGGA
CCTTCCTTCC
CGCGGCCTGC
TGCCGGCTCT
GCGGCCTCTT
CCGCGTCTTC
GCCTTCGCCC
TCAGACGAGT
CGGATCTCCC
TTTGGGCCGC
CTCCCCGCCT
GGAATTAATT
CTGCAGTCGA
GACCTAGAAA
AACATGGAGC
AATCACAAGT
AGCAATACAG
CAGCTACCAA
TGCTGATTGT
GCCTGGCTAG
AAGCACAAGA
GGAGGAGGAG
GTGGGTTTTC
CAGTCACACC
TCAGGTACCT
TTAAGACCAA
TGACTTACAA
GGCAGCTGTA
GATCTTAGCC
ACTTTTTAAA
AGAAAAGAGG
GGACTGGAAG
GGCTAATTCA
CTCCCAACGA
AGACAAGATC
TGCTTTTTGC
TTGTACTGGG
TCTCTCTGGT
TAGACCAGAT
CTGAGCCTGG
GAGCTCTCTG
GCTAACTAGG
GAACCCACTG
CTTAAGCCTC
AATAAAGCTT
GCCTTGAGTG
CTTCAAGTAG
TGTGTGCCCG
TCTGTTGTGT
GACTCTGGTA
ACTAGAGATC
CCTCAGACCC
TTTTAGTCAG
TGTGGAAAAT
CTCTAGCAGT
AGTAGTTCAT
GTCATCTTAT
TATTCAGTAT
TTATAACTTG
CAAAGAAATG
AATATCAGAG
AGTGAGAGGC
CTTGACATTG
TTTAAACCCG
CTGATCAGCC
TCGACTGTGC
CTTCTAGTTG
CCAGCCATCT
GTTGTTTGCC
CCTCCCCCGT
GCCTTCCTTG
ACCCTGGAAG
GTGCCACTCC
CACTGTCCTT
TCCTAATAAA
ATGAGGAAAT
TGCATCGCAT
TGTCTGAGTA
GGTGTCATTC
TATTCTGGGG
GGTGGGGTGG
GGCAGGACAG
CAAGGGGGAG
GATTGGGAAG
ACAATAGCAG
GCATGCTGGG
GATGCGGTGG
GCTCTATGGC
TTCTGAGGCG
GAAAGAACCA
GCTGGGGCTC
TAGGGGGTAT
CCCCACGCGC
CCTGTAGCGG
CGCATTAAGC
GCGGCGGGTG
TGGTGGTTAC
GCGCAGCGTG
ACCGCTACAC
TTGCCAGCGC
CCTAGCGCCC
GCTCCTTTCG
CTTTCTTCCC
TTCCTTTCTC
GCCACGTTCG
CCGGCTTTCC
CCGTCAAGCT
CTAAATCGGG
GGCTCCCTTT
AGGGTTCCGA
TTTAGTGCTT
TACGGCACCT
CGACCCCAAA
AAACTTGATT
AGGGTGATGG
TTCACGTAGT
GGGCCATCGC
CCTGATAGAC
GGTTTTTCGC
CCTTTGACGT
TGGAGTCCAC
GTTCTTTAAT
AGTGGACTCT
TGTTCCAAAC
TGGAACAACA
CTCAACCCTA
TCTCGGTCTA
TTCTTTTGAT
TTATAAGGGA
TTTTGCCGAT
TTCGGCCTAT
TGGTTAAAAA
ATGAGCTGAT
TTAACAAAAA
TTTAACGCGA
ATTAATTCTG
TGGAATGTGT
GTCAGTTAGG
GTGTGGAAAG
TCCCCAGGCT
CCCCAGCAGG
CAGAAGTATG
CAAAGCATGC
ATCTCAATTA
GTCAGCAACC
AGGTGTGGAA
AGTCCCCAGG
CTCCCCAGCA
GGCAGAAGTA
TGCAAAGCAT
GCATCTCAAT
TAGTCAGCAA
CCATAGTCCC
GCCCCTAACT
CCGCCCATCC
CGCCCCTAAC
TCCGCCCAGT
TCCGCCCATT
CTCCGCCCCA
TGGCTGACTA
ATTTTTTTTA
TTTATGCAGA
GGCCGAGGCC
GCCTCTGCCT
CTGAGCTATT
CCAGAAGTAG
TGAGGAGGCT
TTTTTGGAGG
CCTAGGCTTT
TGCAAAAAGC
TCCCGGGAGC
TTGTATATCC
ATTTTCGGAT
CTGATCAGCA
CGTGATGAAA
AAGCCTGAAC
TCACCGCGAC
GTCTGTCGAG
AAGTTTCTGA
TCGAAAAGTT
CGACAGCGTC
TCCGACCTGA
TGCAGCTCTC
GGAGGGCGAA
GAATCTCGTG
CTTTCAGCTT
CGATGTAGGA
GGGCGTGGAT
ATGTCCTGCG
GGTAAATAGC
TGCGCCGATG
GTTTCTACAA
AGATCGTTAT
GTTTATCGGC
ACTTTGCATC
GGCCGCGCTC
CCGATTCCGG
AAGTGCTTGA
CATTGGGGAA
TTCAGCGAGA
GCCTGACCTA
TTGCATCTCC
CGCCGTGCAC
AGGGTGTCAC
GTTGCAAGAC
CTGCCTGAAA
CCGAACTGCC
CGCTGTTCTG
CAGCCGGTCG
CGGAGGCCAT
GGATGCGATC
GCTGCGGCCG
ATCTTAGCCA
GACGAGCGGG
TTCGGCCCAT
TCGGACCGCA
AGGAATCGGT
CAATACACTA
CATGGCGTGA
TTTCATATGC
GCGATTGCTG
ATCCCCATGT
GTATCACTGG
CAAACTGTGA
TGGACGACAC
CGTCAGTGCG
TCCGTCGCGC
AGGCTCTCGA
TGAGCTGATG
CTTTGGGCCG
AGGACTGCCC
CGAAGTCCGG
CACCTCGTGC
ACGCGGATTT
CGGCTCCAAC
AATGTCCTGA
CGGACAATGG
CCGCATAACA
GCGGTCATTG
ACTGGAGCGA
GGCGATGTTC
GGGGATTCCC
AATACGAGGT
CGCCAACATC
TTCTTCTGGA
GGCCGTGGTT
GGCTTGTATG
GAGCAGCAGA
CGCGCTACTT
CGAGCGGAGG
CATCCGGAGC
TTGCAGGATC
GCCGCGGCTC
CGGGCGTATA
TGCTCCGCAT
TGGTCTTGAC
CAACTCTATC
AGAGCTTGGT
TGACGGCAAT
TTCGATGATG
CAGCTTGGGC
GCAGGGTCGA
TGCGACGCAA
TCGTCCGATC
CGGAGCCGGG
ACTGTCGGGC
GTACACAAAT
CGCCCGCAGA
AGCGCGGCCG
TCTGGACCGA
TGGCTGTGTA
GAAGTACTCG
CCGATAGTGG
AAACCGACGC
CCCAGCACTC
GTCCGAGGGC
AAAGGAATAG
CACGTGCTAC
GAGATTTCGA
TTCCACCGCC
GCCTTCTATG
AAAGGTTGGG
CTTCGGAATC
GTTTTCCGGG
ACGCCGGCTG
GATGATCCTC
CAGCGCGGGG
ATCTCATGCT
GGAGTTCTTC
GCCCACCCCA
ACTTGTTTAT
TGCAGCTTAT
AATGGTTACA
AATAAAGCAA
TAGCATCACA
AATTTCACAA
ATAAAGCATT
TTTTTCACTG
CATTCTAGTT
GTGGTTTGTC
CAAACTCATC
AATGTATCTT
ATCATGTCTG
TATACCGTCG
ACCTCTAGCT
AGAGCTTGGC
GTAATCATGG
TCATAGCTGT
TTCCTGTGTG
AAATTGTTAT
CCGCTCACAA
TTCCACACAA
CATACGAGCC
GGAAGCATAA
AGTGTAAAGC
CTGGGGTGCC
TAATGAGTGA
GCTAACTCAC
ATTAATTGCG
TTGCGCTCAC
TGCCCGCTTT
CCAGTCGGGA
AACCTGTCGT
GCCAGCTGCA
TTAATGAATC
GGCCAACGCG
CGGGGAGAGG
CGGTTTGCGT
ATTGGGCGCT
CTTCCGCTTC
CTCGCTCACT
GACTCGCTGC
GCTCGGTCGT
TCGGCTGCGG
CGAGCGGTAT
CAGCTCACTC
AAAGGCGGTA
ATACGGTTAT
CCACAGAATC
AGGGGATAAC
GCAGGAAAGA
ACATGTGAGC
AAAAGGCCAG
CAAAAGGCCA
GGAACCGTAA
AAAGGCCGCG
TTGCTGGCGT
TTTTCCATAG
GCTCCGCCCC
CCTGACGAGC
ATCACAAAAA
TCGACGCTCA
AGTCAGAGGT
GGCGAAACCC
GACAGGACTA
TAAAGATACC
AGGCGTTTCC
CCCTGGAAGC
TCCCTCGTGC
GCTCTCCTGT
TCCGACCCTG
CCGCTTACCG
GATACCTGTC
CGCCTTTCTC
CCTTCGGGAA
GCGTGGCGCT
TTCTCATAGC
TCACGCTGTA
GGTATCTCAG
TTCGGTGTAG
GTCGTTCGCT
CCAAGCTGGG
CTGTGTGCAC
GAACCCCCCG
TTCAGCCCGA
CCGCTGCGCC
TTATCCGGTA
ACTATCGTCT
TGAGTCCAAC
CCGGTAAGAC
ACGACTTATC
GCCACTGGCA
GCAGCCACTG
GTAACAGGAT
TAGCAGAGCG
AGGTATGTAG
GCGGTGCTAC
AGAGTTCTTG
AAGTGGTGGC
CTAACTACGG
CTACACTAGA
AGAACAGTAT
TTGGTATCTG
CGCTCTGCTG
AAGCCAGTTA
CCTTCGGAAA
AAGAGTTGGT
AGCTCTTGAT
CCGGCAAACA
AACCACCGCT
GGTAGCGGTG
GTTTTTTTGT
TTGCAAGCAG
CAGATTACGC
GCAGAAAAAA
AGGATCTCAA
GAAGATCCTT
TGATCTTTTC
TACGGGGTCT
GACGCTCAGT
GGAACGAAAA
CTCACGTTAA
GGGATTTTGG
TCATGAGATT
ATCAAAAAGG
ATCTTCACCT
AGATCCTTTT
AAATTAAAAA
TGAAGTTTTA
AATCAATCTA
AAGTATATAT
GAGTAAACTT
GGTCTGACAG
TTACCAATGC
TTAATCAGTG
AGGCACCTAT
CTCAGCGATC
TGTCTATTTC
GTTCATCCAT
AGTTGCCTGA
CTCCCCGTCG
TGTAGATAAC
TACGATACGG
GAGGGCTTAC
CATCTGGCCC
CAGTGCTGCA
ATGATACCGC
GAGACCCACG
CTCACCGGCT
CCAGATTTAT
CAGCAATAAA
CCAGCCAGCC
GGAAGGGCCG
AGCGCAGAAG
TGGTCCTGCA
ACTTTATCCG
CCTCCATCCA
GTCTATTAAT
TGTTGCCGGG
AAGCTAGAGT
AAGTAGTTCG
CCAGTTAATA
GTTTGCGCAA
CGTTGTTGCC
ATTGCTACAG
GCATCGTGGT
GTCACGCTCG
TCGTTTGGTA
TGGCTTCATT
CAGCTCCGGT
TCCCAACGAT
CAAGGCGAGT
TACATGATCC
CCCATGTTGT
GCAAAAAAGC
GGTTAGCTCC
TTCGGTCCTC
CGATCGTTGT
CAGAAGTAAG
TTGGCCGCAG
TGTTATCACT
CATGGTTATG
GCAGCACTGC
ATAATTCTCT
TACTGTCATG
CCATCCGTAA
GATGCTTTTC
TGTGACTGGT
GAGTACTCAA
CCAAGTCATT
CTGAGAATAG
TGTATGCGGC
GACCGAGTTG
CTCTTGCCCG
GCGTCAATAC
GGGATAATAC
CGCGCCACAT
AGCAGAACTT
TAAAAGTGCT
CATCATTGGA
AAACGTTCTT
CGGGGCGAAA
ACTCTCAAGG
ATCTTACCGC
TGTTGAGATC
CAGTTCGATG
TAACCCACTC
GTGCACCCAA
CTGATCTTCA
GCATCTTTTA
CTTTCACCAG
CGTTTCTGGG
TGAGCAAAAA
CAGGAAGGCA
AAATGCCGCA
AAAAAGGGAA
TAAGGGCGAC
ACGGAAATGT
TGAATACTCA
TACTCTTCCT
TTTTCAATAT
TATTGAAGCA
TTTATCAGGG
TTATTGTCTC
ATGAGCGGAT
ACATATTTGA
ATGTATTTAG
AAAAATAAAC
AAATAGGGGT
TCCGCGCACA
TTTCCCCGAA
AAGTGCCACC
TGACGTCGAC
GGATCGGGAG
ATCAACTTGT
TTATTGCAGC
TTATAATGGT
TACAAATAAA
GCAATAGCAT
CACAAATTTC
ACAAATAAAG
CATTTTTTTC
ACTGCATTCT
AGTTGTGGTT
TGTCCAAACT
CATCAATGTA
TCTTATCATG
TCTGGATCAA
CTGGATAACT
CAAGCTAACC
AAAATCATCC
CAAACTTCCC
ACCCCATACC
CTATTACCAC
TGCCAATTAC
CTGTGGTTTC
ATTTACTCTA
AACCTGTGAT
TCCTCTGAAT
TATTTTCATT
TTAAAGAAAT
TGTATTTGTT
AAATATGTAC
TACAAACTTA
GTAGT