名称: | pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI |
---|---|
别称: | Plasmid#115267 |
基因长: | |
质粒宿主: | 大肠杆菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | T7 |
复制子: | pBR322 |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GGAATTGTGA
GCGGATAACA
ATTCCCCTCT
AGAAATAATT
TTGTTTAACT
TTAAGAAGGA
GATATACCAT
GGGCAGCAGC
CATCATCATC
ATCATCACAG
CAGCGGCCTG
GTGCCGCGCG
GCAGCCATAT
GGCTAGCTGG
AGCCATCCGC
AGTTTGAAAA
AGGTGGTGGT
AGCGGTGGTG
GTTCAGGTGG
TAGTGCATGG
TCACACCCTC
AGTTTGAGAA
AATGTCGGAC
TCAGAAGTCA
ATCAAGAAGC
TAAGCCAGAG
GTCAAGCCAG
AAGTCAAGCC
TGAGACTCAC
ATCAATTTAA
AGGTGTCCGA
TGGATCTTCA
GAGATCTTCT
TCAAGATCAA
AAAGACCACT
CCTTTAAGAA
GGCTGATGGA
AGCGTTCGCT
AAAAGACAGG
GTAAGGAAAT
GGACTCCTTA
AGATTCTTGT
ACGACGGTAT
TAGAATCCAA
GCTGATCAGA
CCCCTGAAGA
TTTGGACATG
GAGGATAACG
ATATTATTGA
GGCTCACAGA
GAACAGATTG
GTGGATCTAT
GAAGGTGACA
AAAGTGGGCG
GCATCAGCCA
CAAGAAGTAT
ACCTCCGAGG
GCAGGCTGGT
GAAGTCTGAG
AGCGAGGAGA
ACAGGACCGA
CGAGCGCCTG
TCCGCCCTGC
TGAATATGAG
GCTGGATATG
TATATCAAGA
ACCCCAGCTC
CACCGAGACA
AAGGAGAATC
AGAAGCGCAT
CGGCAAGCTG
AAGAAGTTCT
TTTCCAACAA
GATGGTGTAT
CTGAAGGACA
ATACACTGTC
TCTGAAGAAT
GGCAAGAAGG
AGAACATCGA
CCGGGAGTAC
TCTGAGACAG
ATATCCTGGA
GAGCGACGTG
AGAGATAAGA
AGAACTTCGC
CGTGCTGAAG
AAGATCTACC
TGAATGAGAA
CGTGAACAGC
GAGGAGCTGG
AGGTGTTCCG
GAATGATATC
AAGAAGAAGC
TGAACAAGAT
CAATTCTCTG
AAGTACAGCT
TTGAGAAGAA
CAAGGCCAAT
TATCAGAAGA
TCAACGAGAA
CAATATCGAG
AAGGTGGAGG
GCAAGAGCAA
GAGAAATATC
ATCTACGACT
ACTATAGGGA
GTCCGCCAAG
CGGGACGCCT
ACGTGAGCAA
CGTGAAGGAG
GCCTTTGACA
AGCTGTATAA
GGAGGAGGAT
ATCGCCAAGC
TGGTGCTGGA
GATCGAGAAT
CTGACCAAGC
TGGAGAAGTA
CAAGATCAGG
GAGTTCTATC
ACGAGATCAT
CGGCCGCAAG
AATGACAAGG
AGAACTTTGC
CAAGATCATC
TACGAGGAGA
TCCAGAACGT
GAACAATATG
AAGGAGCTGA
TCGAGAAGGT
GCCTGATATG
TCCGAGCTGA
AGAAGTCTCA
GGTGTTCTAT
AAGTACTATC
TGGACAAGGA
GGAGCTGAAC
GATAAGAATA
TCAAGTACGC
CTTCTGCCAC
TTTGTGGAGA
TCGAGATGAG
CCAGCTGCTG
AAGAACTACG
TGTATAAGAG
GCTGTCCAAC
ATCTCTAATG
ACAAGATCAA
GCGCATCTTT
GAGTATCAGA
ATCTGAAGAA
GCTGATCGAG
AATAAGCTGC
TGAACAAGCT
GGATACATAC
GTGAGAAATT
GTGGCAAGTA
TAACTACTAT
CTGCAGGACG
GCGAGATCGC
CACCTCCGAT
TTCATCGCCC
GGAACAGACA
GAATGAGGCC
TTTCTGCGGA
ACATCATCGG
CGTGTCTAGC
GTGGCCTACT
TCTCTCTGAG
AAATATCCTG
GAGACAGAAA
ACGAGAATGA
CATCACAGGC
AGGATGCGCG
GCAAGACCGT
GAAGAACAAT
AAGGGCGAGG
AGAAGTACGT
GTCTGGCGAG
GTGGATAAGA
TCTACAACGA
GAACAAGAAG
AATGAGGTGA
AGGAGAACCT
GAAGATGTTC
TACAGCTATG
ACTTTAACAT
GGATAACAAG
AATGAGATCG
AGGACTTCTT
TGCCAATATC
GATGAGGCCA
TCTCCTCTAT
CAGGCACGGC
ATCGTGCACT
TCAACCTGGA
GCTGGAGGGC
AAGGACATCT
TCGCCTTTAA
GAATATCGCC
CCAAGCGAGA
TCTCCAAGAA
GATGTTTCAG
AACGAGATCA
ATGAGAAGAA
GCTGAAGCTG
AAGATCTTCC
GGCAGCTGAA
CTCCGCCAAC
GTGTTCAGAT
ACCTGGAGAA
GTATAAGATC
CTGAACTACC
TGAAGCGGAC
AAGATTCGAG
TTTGTGAACA
AGAATATCCC
CTTCGTGCCT
TCTTTTACCA
AGCTGTATAG
CCGCATCGAC
GATCTGAAGA
ACTCTCTGGG
CATCTACTGG
AAAACCCCTA
AGACAAACGA
CGATAATAAG
ACAAAGGAGA
TCATCGACGC
CCAGATCTAT
CTGCTGAAGA
ACATCTACTA
TGGCGAGTTC
CTGAATTACT
TTATGTCTAA
CAATGGCAAC
TTCTTTGAGA
TCAGCAAGGA
GATCATCGAG
CTGAACAAGA
ATGACAAGAG
GAACCTGAAA
ACCGGCTTCT
ACAAGCTGCA
GAAGTTTGAG
GATATCCAGG
AGAAGATCCC
AAAGGAGTAC
CTGGCCAATA
TCCAGTCCCT
GTATATGATC
AACGCCGGCA
ATCAGGACGA
GGAGGAGAAG
GACACATACA
TCGATTTCAT
CCAGAAGATC
TTCCTGAAGG
GCTTTATGAC
CTACCTGGCC
AACAATGGCC
GCCTGTCCCT
GATCTATATC
GGCTCTGACG
AGGAGACAAA
CACAAGCCTG
GCCGAGAAGA
AGCAGGAGTT
CGATAAGTTT
CTGAAGAAGT
ACGAGCAGAA
CAATAACATC
AAGATCCCCT
ATGAGATCAA
TGAGTTCCTG
AGGGAGATCA
AGCTGGGCAA
CATCCTGAAG
TACACAGAGC
GCCTGAATAT
GTTTTATCTG
ATCCTGAAGC
TGCTGAATCA
CAAGGAGCTG
ACCAACCTGA
AGGGCAGCCT
GGAGAAGTAC
CAGTCCGCCA
ACAAGGAGGA
GGCCTTCTCC
GACCAGCTGG
AGCTGATCAA
CCTGCTGAAT
CTGGATAATA
ACAGGGTGAC
AGAGGACTTT
GAGCTGGAGG
CCGATGAGAT
CGGCAAGTTC
CTGGACTTTA
ACGGCAATAA
GGTGAAGGAT
AACAAGGAGC
TGAAGAAGTT
CGACACCAAT
AAGATCTACT
TTGATGGCGA
GAACATCATC
AAGCACCGCG
CCTTCTACAA
TATCAAGAAG
TATGGCATGC
TGAACCTGCT
GGAGAAGATC
GCCGACAAGG
CCGGCTACAA
GATCAGCATC
GAGGAGCTGA
AGAAGTATTC
CAACAAGAAG
AATGAGATCG
AGAAGAATCA
CAAGATGCAG
GAGAACCTGC
ACCGGAAGTA
TGCCAGGCCA
CGCAAGGATG
AGAAGTTCAC
AGACGAGGAT
TACGAGAGCT
ATAAGCAGGC
CATCGAGAAC
ATCGAGGAGT
ACACCCACCT
GAAGAATAAG
GTGGAGTTTA
ACGAGCTGAA
TCTGCTGCAG
GGCCTGCTGC
TGAGAATCCT
GCACAGACTG
GTGGGCTACA
CATCCATCTG
GGAGCGGGAC
CTGCGGTTCA
GACTGAAGGG
CGAGTTTCCC
GAGAACCAGT
ATATCGAGGA
GATCTTCAAC
TTTGAGAATA
AGAAGAACGT
GAAGTACAAG
GGCGGCCAGA
TCGTGGAGAA
GTACATCAAG
TTCTATAAGG
AGCTGCACCA
GAATGACGAG
GTGAAGATCA
ACAAGTATAG
CTCCGCCAAT
ATCAAGGTGC
TGAAGCAGGA
GAAGAAGGAT
CTGTATATCC
GGAACTATAT
CGCCCACTTT
AATTACATCC
CTCACGCCGA
GATCAGCCTG
CTGGAGGTGC
TGGAGAACCT
GCGGAAGCTG
CTGAGCTATG
ACAGAAAGCT
GAAGAATGCC
GTGATGAAGT
CCGTGGTGGA
TATCCTGAAG
GAGTACGGCT
TCGTGGCCAC
ATTTAAGATC
GGCGCCGACA
AGAAGATCGG
CATCCAGACC
CTGGAGTCTG
AGAAGATCGT
GCACCTGAAG
AACCTGAAGA
AGAAGAAGCT
GATGACCGAT
AGAAATAGCG
AGGAGCTGTG
CAAGCTGGTG
AAGATCATGT
TCGAGTACAA
GATGGAGGAG
AAGAAGTCCG
AGAACTAAGG
CCGCACTCGA
GGCCCGAAAG
GAAGCTGAGT
TGGCTGCTGC
CACCGCTGAG
CAATAACTAG
CATAACCCCT
TGGGGCCTCT
AAACGGGTCT
TGAGGGGTTT
TTTGCTGAAA
GGAGGAACTA
TATCCGGATA
TCCCGCAAGA
GGCCCGGCAG
TACCGGCATA
ACCAAGCCTA
TGCCTACAGC
ATCCAGGGTG
ACGGTGCCGA
GGATGACGAT
GAGCGCATTG
TTAGATTTCA
TACACGGTGC
CTGACTGCGT
TAGCAATTTA
ACTGTGATAA
ACTACCGCAT
TAAAGCTTAT
CGATGATAAG
CTGTCAAACA
TGAGAATTCT
TGAAGACGAA
AGGGCCTCGT
GATACGCCTA
TTTTTATAGG
TTAATGTCAT
GATAATAATG
GTTTCTTAGA
CGTCAGGTGG
CACTTTTCGG
GGAAATGTGC
GCGGAACCCC
TATTTGTTTA
TTTTTCTAAA
TACATTCAAA
TATGTATCCG
CTCATGAGAC
AATAACCCTG
ATAAATGCTT
CAATAATATT
GAAAAAGGAA
GAGTATGAGT
ATTCAACATT
TCCGTGTCGC
CCTTATTCCC
TTTTTTGCGG
CATTTTGCCT
TCCTGTTTTT
GCTCACCCAG
AAACGCTGGT
GAAAGTAAAA
GATGCTGAAG
ATCAGTTGGG
TGCACGAGTG
GGTTACATCG
AACTGGATCT
CAACAGCGGT
AAGATCCTTG
AGAGTTTTCG
CCCCGAAGAA
CGTTTTCCAA
TGATGAGCAC
TTTTAAAGTT
CTGCTATGTG
GCGCGGTATT
ATCCCGTGTT
GACGCCGGGC
AAGAGCAACT
CGGTCGCCGC
ATACACTATT
CTCAGAATGA
CTTGGTTGAG
TACTCACCAG
TCACAGAAAA
GCATCTTACG
GATGGCATGA
CAGTAAGAGA
ATTATGCAGT
GCTGCCATAA
CCATGAGTGA
TAACACTGCG
GCCAACTTAC
TTCTGACAAC
GATCGGAGGA
CCGAAGGAGC
TAACCGCTTT
TTTGCACAAC
ATGGGGGATC
ATGTAACTCG
CCTTGATCGT
TGGGAACCGG
AGCTGAATGA
AGCCATACCA
AACGACGAGC
GTGACACCAC
GATGCCTGCA
GCAATGGCAA
CAACGTTGCG
CAAACTATTA
ACTGGCGAAC
TACTTACTCT
AGCTTCCCGG
CAACAATTAA
TAGACTGGAT
GGAGGCGGAT
AAAGTTGCAG
GACCACTTCT
GCGCTCGGCC
CTTCCGGCTG
GCTGGTTTAT
TGCTGATAAA
TCTGGAGCCG
GTGAGCGTGG
GTCACGCGGT
ATCATTGCAG
CACTGGGGCC
AGATGGTAAG
CCCTCCCGTA
TCGTAGTTAT
CTACACGACG
GGGAGTCAGG
CAACTATGGA
TGAACGAAAT
AGACAGATCG
CTGAGATAGG
TGCCTCACTG
ATTAAGCATT
GGTAACTGTC
AGACCAAGTT
TACTCATATA
TACTTTAGAT
TGATTTAAAA
CTTCATTTTT
AATTTAAAAG
GATCTAGGTG
AAGATCCTTT
TTGATAATCT
CATGACCAAA
ATCCCTTAAC
GTGAGTTTTC
GTTCCACTGA
GCGTCAGACC
CCGTAGAAAA
GATCAAAGGA
TCTTCTTGAG
ATCCTTTTTT
TCTGCGCGTA
ATCTGCTGCT
TGCAAACAAA
AAAACCACCG
CTACCAGCGG
TGGTTTGTTT
GCCGGATCAA
GAGCTACCAA
CTCTTTTTCC
GAAGGTAACT
GGCTTCAGCA
GAGCGCAGAT
ACCAAATACT
GTCCTTCTAG
TGTAGCCGTA
GTTAGGCCAC
CACTTCAAGA
ACTCTGTAGC
ACCGCCTACA
TACCTCGCTC
TGCTAATCCT
GTTACCAGTG
GCTGCTGCCA
GTGGCGATAA
GTCGTGTCTT
ACCGGGTTGG
ACTCAAGACG
ATAGTTACCG
GATAAGGCGC
AGCGGTCGGG
CTGAACGGGG
GGTTCGTGCA
CACAGCCCAG
CTTGGAGCGA
ACGACCTACA
CCGAACTGAG
ATACCTACAG
CGTGAGCTAT
GAGAAAGCGC
CACGCTTCCC
GAAGGGAGAA
AGGCGGACAG
GTATCCGGTA
AGCGGCAGGG
TCGGAACAGG
AGAGCGCACG
AGGGAGCTTC
CAGGGGGAAA
CGCCTGGTAT
CTTTATAGTC
CTGTCGGGTT
TCGCCACCTC
TGACTTGAGC
GTCGATTTTT
GTGATGCTCG
TCAGGGGGGC
GGAGCCTATG
GAAAAACGCC
AGCAACGCGG
CCTTTTTACG
GTTCCTGGCC
TTTTGCTGGC
CTTTTGCTCA
CATGTTCTTT
CCTGCGTTAT
CCCCTGATTC
TGTGGATAAC
CGTATTACCG
CCTTTGAGTG
AGCTGATACC
GCTCGCCGCA
GCCGAACGAC
CGAGCGCAGC
GAGTCAGTGA
GCGAGGAAGC
GGAAGAGCGC
CTGATGCGGT
ATTTTCTCCT
TACGCATCTG
TGCGGTATTT
CACACCGCAA
TGGTGCACTC
TCAGTACAAT
CTGCTCTGAT
GCCGCATAGT
TAAGCCAGTA
TACACTCCGC
TATCGCTACG
TGACTGGGTC
ATGGCTGCGC
CCCGACACCC
GCCAACACCC
GCTGACGCGC
CCTGACGGGC
TTGTCTGCTC
CCGGCATCCG
CTTACAGACA
AGCTGTGACC
GTCTCCGGGA
GCTGCATGTG
TCAGAGGTTT
TCACCGTCAT
CACCGAAACG
CGCGAGGCAG
CTGCGGTAAA
GCTCATCAGC
GTGGTCGTGA
AGCGATTCAC
AGATGTCTGC
CTGTTCATCC
GCGTCCAGCT
CGTTGAGTTT
CTCCAGAAGC
GTTAATGTCT
GGCTTCTGAT
AAAGCGGGCC
ATGTTAAGGG
CGGTTTTTTC
CTGTTTGGTC
ACTGATGCCT
CCGTGTAAGG
GGGATTTCTG
TTCATGGGGG
TAATGATACC
GATGAAACGA
GAGAGGATGC
TCACGATACG
GGTTACTGAT
GATGAACATG
CCCGGTTACT
GGAACGTTGT
GAGGGTAAAC
AACTGGCGGT
ATGGATGCGG
CGGGACCAGA
GAAAAATCAC
TCAGGGTCAA
TGCCAGCGCT
TCGTTAATAC
AGATGTAGGT
GTTCCACAGG
GTAGCCAGCA
GCATCCTGCG
ATGCAGATCC
GGAACATAAT
GGTGCAGGGC
GCTGACTTCC
GCGTTTCCAG
ACTTTACGAA
ACACGGAAAC
CGAAGACCAT
TCATGTTGTT
GCTCAGGTCG
CAGACGTTTT
GCAGCAGCAG
TCGCTTCACG
TTCGCTCGCG
TATCGGTGAT
TCATTCTGCT
AACCAGTAAG
GCAACCCCGC
CAGCCTAGCC
GGGTCCTCAA
CGACAGGAGC
ACGATCATGC
GCACCCGTGG
CCAGGACCCA
ACGCTGCCCG
AGATGCGCCG
CGTGCGGCTG
CTGGAGATGG
CGGACGCGAT
GGATATGTTC
TGCCAAGGGT
TGGTTTGCGC
ATTCACAGTT
CTCCGCAAGA
ATTGATTGGC
TCCAATTCTT
GGAGTGGTGA
ATCCGTTAGC
GAGGTGCCGC
CGGCTTCCAT
TCAGGTCGAG
GTGGCCCGGC
TCCATGCACC
GCGACGCAAC
GCGGGGAGGC
AGACAAGGTA
TAGGGCGGCG
CCTACAATCC
ATGCCAACCC
GTTCCATGTG
CTCGCCGAGG
CGGCATAAAT
CGCCGTGACG
ATCAGCGGTC
CAATGATCGA
AGTTAGGCTG
GTAAGAGCCG
CGAGCGATCC
TTGAAGCTGT
CCCTGATGGT
CGTCATCTAC
CTGCCTGGAC
AGCATGGCCT
GCAACGCGGG
CATCCCGATG
CCGCCGGAAG
CGAGAAGAAT
CATAATGGGG
AAGGCCATCC
AGCCTCGCGT
CGCGAACGCC
AGCAAGACGT
AGCCCAGCGC
GTCGGCCGCC
ATGCCGGCGA
TAATGGCCTG
CTTCTCGCCG
AAACGTTTGG
TGGCGGGACC
AGTGACGAAG
GCTTGAGCGA
GGGCGTGCAA
GATTCCGAAT
ACCGCAAGCG
ACAGGCCGAT
CATCGTCGCG
CTCCAGCGAA
AGCGGTCCTC
GCCGAAAATG
ACCCAGAGCG
CTGCCGGCAC
CTGTCCTACG
AGTTGCATGA
TAAAGAAGAC
AGTCATAAGT
GCGGCGACGA
TAGTCATGCC
CCGCGCCCAC
CGGAAGGAGC
TGACTGGGTT
GAAGGCTCTC
AAGGGCATCG
GTCGAGATCC
CGGTGCCTAA
TGAGTGAGCT
AACTTACATT
AATTGCGTTG
CGCTCACTGC
CCGCTTTCCA
GTCGGGAAAC
CTGTCGTGCC
AGCTGCATTA
ATGAATCGGC
CAACGCGCGG
GGAGAGGCGG
TTTGCGTATT
GGGCGCCAGG
GTGGTTTTTC
TTTTCACCAG
TGAGACGGGC
AACAGCTGAT
TGCCCTTCAC
CGCCTGGCCC
TGAGAGAGTT
GCAGCAAGCG
GTCCACGCTG
GTTTGCCCCA
GCAGGCGAAA
ATCCTGTTTG
ATGGTGGTTA
ACGGCGGGAT
ATAACATGAG
CTGTCTTCGG
TATCGTCGTA
TCCCACTACC
GAGATATCCG
CACCAACGCG
CAGCCCGGAC
TCGGTAATGG
CGCGCATTGC
GCCCAGCGCC
ATCTGATCGT
TGGCAACCAG
CATCGCAGTG
GGAACGATGC
CCTCATTCAG
CATTTGCATG
GTTTGTTGAA
AACCGGACAT
GGCACTCCAG
TCGCCTTCCC
GTTCCGCTAT
CGGCTGAATT
TGATTGCGAG
TGAGATATTT
ATGCCAGCCA
GCCAGACGCA
GACGCGCCGA
GACAGAACTT
AATGGGCCCG
CTAACAGCGC
GATTTGCTGG
TGACCCAATG
CGACCAGATG
CTCCACGCCC
AGTCGCGTAC
CGTCTTCATG
GGAGAAAATA
ATACTGTTGA
TGGGTGTCTG
GTCAGAGACA
TCAAGAAATA
ACGCCGGAAC
ATTAGTGCAG
GCAGCTTCCA
CAGCAATGGC
ATCCTGGTCA
TCCAGCGGAT
AGTTAATGAT
CAGCCCACTG
ACGCGTTGCG
CGAGAAGATT
GTGCACCGCC
GCTTTACAGG
CTTCGACGCC
GCTTCGTTCT
ACCATCGACA
CCACCACGCT
GGCACCCAGT
TGATCGGCGC
GAGATTTAAT
CGCCGCGACA
ATTTGCGACG
GCGCGTGCAG
GGCCAGACTG
GAGGTGGCAA
CGCCAATCAG
CAACGACTGT
TTGCCCGCCA
GTTGTTGTGC
CACGCGGTTG
GGAATGTAAT
TCAGCTCCGC
CATCGCCGCT
TCCACTTTTT
CCCGCGTTTT
CGCAGAAACG
TGGCTGGCCT
GGTTCACCAC
GCGGGAAACG
GTCTGATAAG
AGACACCGGC
ATACTCTGCG
ACATCGTATA
ACGTTACTGG
TTTCACATTC
ACCACCCTGA
ATTGACTCTC
TTCCGGGCGC
TATCATGCCA
TACCGCGAAA
GGTTTTGCGC
CATTCGATGG
TGTCCGGGAT
CTCGACGCTC
TCCCTTATGC
GACTCCTGCA
TTAGGAAGCA
GCCCAGTAGT
AGGTTGAGGC
CGTTGAGCAC
CGCCGCCGCA
AGGAATGGTG
CATGCAAGGA
GATGGCGCCC
AACAGTCCCC
CGGCCACGGG
GCCTGCCACC
ATACCCACGC
CGAAACAAGC
GCTCATGAGC
CCGAAGTGGC
GAGCCCGATC
TTCCCCATCG
GTGATGTCGG
CGATATAGGC
GCCAGCAACC
GCACCTGTGG
CGCCGGTGAT
GCCGGCCACG
ATGCGTCCGG
CGTAGAGGAT
CGAGATCTCG
ATCCCGCGAA
ATTAATACGA
CTCACTATAG
G