名称: | pCS2-Cas9 |
---|---|
别称: | Plasmid#47322 |
基因长: | |
质粒宿主: | 哺乳细胞,鱼类 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | CMV-F: |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AGCGCCCAAT
ACGCAAACCG
CCTCTCCCCG
CGCGTTGGCC
GATTCATTAA
TGCAGCTGGC
ACGACAGGTT
TCCCGACTGG
AAAGCGGGCA
GTGAGCGCAA
CGCAATTAAT
GTGAGTTAGC
TCACTCATTA
GGCACCCCAG
GCTTTACACT
TTATGCTCCC
GGCTCGTATG
TTGTGTGGAA
TTGTGAGCGG
ATAACAATTT
CACACAGGAA
ACAGCTATGA
CCATGATTAC
GCCAAGCGCG
CAATTAACCC
TCACTAAAGG
GAACAAAAGC
TGGGTACCGG
GCCCAATGCA
TTGGCGCCGC
GGCCGCGAAT
TAAAAAACCT
CCCACACCTC
CCCCTGAACC
TGAAACATAA
AATGAATGCA
ATTGTTGTTG
TTAACTTGTT
TATTGCAGCT
TATAATGGTT
ACAAATAAAG
CAATAGCATC
ACAAATTTCA
CAAATAAAGC
ATTTTTTTCA
CTGCATTCTA
GTTGTGGTTT
GTCCAAACTC
ATCAATGTAT
CTTATCATGT
CTGGATCTAC
GTAATACGAC
TCACTATAGT
TCTAGATTAC
ACCTTCCTCT
TCTTCTTGGG
GTCAGCCCTG
CTGTCTCCAC
CGAGCTGAGA
GAGGTCGATT
CTTGTTTCAT
AGAGCCCCGT
AATTGACTGA
TGAATCAGTG
TGGCGTCCAG
GACCTCCTTT
GTAGAGGTGT
ACCGCTTTCT
GTCTATGGTG
GTGTCGAAGT
ACTTGAAGGC
TGCAGGCGCG
CCCAAGTTGG
TCAGAGTAAA
CAAGTGGATA
ATGTTTTCTG
CCTGCTCCCT
GATGGGCTTA
TCCCTGTGCT
TATTGTAAGC
AGAAAGCACC
TTATCGAGGT
TAGCGTCGGC
GAGGATCACT
CTTTTGGAGA
ATTCGCTTAT
TTGCTCGATG
ATCTCATCAA
GGTAGTGTTT
GTGTTGTTCC
ACGAACAGCT
GCTTCTGCTC
ATTATCTTCG
GGAGACCCTT
TGAGCTTTTC
ATAGTGGCTG
GCCAGATACA
AGAAATTAAC
GTATTTAGAG
GGCAGTGCCA
GCTCGTTACC
TTTCTGCAGC
TCGCCCGCAC
TAGCGAGCAT
TCGTTTCCGG
CCGTTTTCAA
GCTCAAAGAG
AGAGTACTTG
GGAAGCTTAA
TGATGAGGTC
TTTTTTGACC
TCTTTATATC
CTTTCGCCTC
GAGAAAGTCG
ATGGGGTTTT
TTTCGAAGCT
TGATCGCTCC
ATGATTGTGA
TGCCCAGCAG
TTCCTTGACG
CTTTTGAGTT
TTTTAGACTT
CCCTTTCTCC
ACTTTGGCCA
CAACCAGTAC
ACTGTAAGCG
ACTGTAGGAG
AATCGAATCC
GCCGTATTTC
TTGGGGTCCC
AATCTTTTTT
GCGTGCGATC
AGCTTGTCGC
TGTTCCTTTT
CGGGAGGATA
CTTTCCTTGG
AGAAGCCTCC
GGTCTGTACT
TCGGTCTTTT
TAACGATGTT
CACCTGCGGC
ATGGACAGGA
CCTTCCGGAC
TGTCGCGAAA
TCCCTACCCT
TGTCCCACAC
GATTTCTCCT
GTTTCTCCGT
TTGTTTCGAT
AAGTGGTCGC
TTCCGAATCT
CTCCATTGGC
CAGTGTAATC
TCGGTCTTGA
AAAAATTCAT
AATATTGCTG
TAAAAGAAGT
ACTTAGCGGT
GGCCTTGCCT
ATTTCCTGCT
CAGACTTTGC
GATCATTTTC
CTAACATCGT
ACACTTTATA
GTCTCCGTAA
ACAAATTCAG
ATTCAAGCTT
GGGATATTTT
TTGATAAGTG
CAGTGCCTAC
CACTGCATTC
AGGTAGGCAT
CATGCGCATG
GTGGTAATTG
TTGATCTCTC
TCACCTTATA
AAACTGAAAG
TCCTTTCTGA
AATCTGAGAC
CAGCTTAGAC
TTCAGAGTAA
TAACTTTCAC
CTCTCGAATC
AGTTTGTCAT
TTTCATCGTA
CTTGGTGTTC
ATGCGTGAAT
CGAGAATTTG
GGCCACGTGC
TTGGTGATCT
GGCGTGTCTC
AACAAGCTGC
CTTTTGATGA
AGCCGGCTTT
ATCCAACTCA
GACAGGCCAC
CTCGTTCAGC
CTTAGTCAGA
TTATCGAACT
TCCGTTGTGT
GATCAGTTTG
GCGTTCAGCA
GCTGCCGCCA
ATAATTTTTC
ATTTTCTTGA
CAACTTCTTC
TGAGGGGACG
TTATCACTCT
TCCCTCTATT
TTTATCGGAT
CTTGTCAACA
CTTTATTATC
AATAGAATCA
TCTTTGAGAA
AAGACTGGGG
CACGATATGA
TCCACGTCGT
AGTCGGAGAG
CCGATTGATG
TCCAGTTCCT
GATCCACGTA
CATGTCCCTG
CCGTTCTGCA
GGTAGTACAG
GTAGAGCTTC
TCATTCTGAA
GCTGGGTGTT
TTCAACTGGG
TGTTCCTTAA
GGATTTGGGA
CCCCAGTTCT
TTTATACCCT
CTTCAATCCT
CTTCATCCTT
TCCCTACTGT
TCTTCTGTCC
CTTCTGGGTA
GTTTGGTTCT
CTCGGGCCAT
CTCGATAACG
ATATTCTCGG
GCTTATGCCT
TCCCATTACT
TTGACGAGTT
CATCCACGAC
CTTAACGGTC
TGCAGTATTC
CCTTTTTGAT
AGCTGGGCTA
CCTGCAAGAT
TAGCGATGTG
CTCGTGAAGA
CTGTCCCCCT
GGCCAGAAAC
TTGTGCTTTC
TGGATGTCCT
CCTTAAAGGT
GAGAGAGTCA
TCATGGATCA
ACTGCATGAA
GTTCCGGTTG
GCAAATCCAT
CGGACTTAAG
AAAATCCAGG
ATTGTCTTTC
CACTCTGCTT
GTCTCGGATC
CCATTGATCA
GTTTTCTTGA
CAGCCGCCCC
CATCCTGTAT
ATCGGCGCCT
CTTGAGCTGT
TTCATGACTT
TGTCGTCGAA
GAGATGAGCG
TAAGTTTTCA
AGCGTTCTTC
AATCATCTCC
CTATCTTCAA
ACAACGTAAG
GGTGAGGACA
ATGTCCTCAA
GAATGTCCTC
GTTCTCCTCA
TTGTCCAGGA
AGTCCTTGTC
TTTAATGATT
TTCAGGAGAT
CGTGATACGT
TCCCAGGGAT
GCGTTGAAGC
GATCCTCCAC
TCCGCTGATT
TCAACAGAGT
CGAAACATTC
AATCTTTTTG
AAATAGTCTT
CTTTGAGCTG
TTTCACGGTA
ACTTTCCGGT
TCGTCTTGAA
GAGGAGGTCC
ACGATAGCTT
TCTTCTGCTC
TCCAGACAGG
AATGCTGGCT
TTCTCATCCC
TTCTGTGACG
TATTTGACCT
TGGTGAGCTC
GTTATAAACT
GTGAAGTACT
CGTACAGCAG
AGAGTGTTTA
GGAAGCACCT
TTTCGTTAGG
CAGATTTTTA
TCAAAGTTAG
TCATCCTTTC
GATGAAGGAC
TGGGCAGAGG
CCCCCTTATC
CACGACTTCC
TCGAAGTTCC
AGGGAGTGAT
GGTCTCTTCT
GATTTGCGAG
TCATCCACGC
GAATCTGGAA
TTTCCCCGGG
CGAGGGGGCC
TACATAGTAG
GGTATCCGAA
ATGTGAGGAT
TTTCTCAATC
TTTTCCCTGT
TATCTTTCAA
AAAGGGGTAG
AAATCCTCTT
GCCGCCTGAG
GATAGCGTGC
AGTTCGCCCA
GGTGAATCTG
GTGGGGGATG
CTTCCATTGT
CGAAAGTGCG
CTGTTTGCGC
AACAGATCTT
CTCTGTTAAG
CTTTACCAGC
AGCTCCTCGG
TGCCGTCCAT
TTTTTCCAAG
ATGGGCTTAA
TAAATTTGTA
AAATTCCTCC
TGGCTTGCTC
CGCCGTCAAT
GTATCCGGCG
TAGCCATTTT
TAGACTGATC
GAAGAAAATT
TCCTTGTACT
TCTCAGGCAG
TTGCTGTCTG
ACAAGGGCCT
TCAGCAAAGT
CAAGTCTTGG
TGGTGCTCAT
CATAGCGCTT
GATCATACTA
GCGCTCAGCG
GAGCTTTGGT
GATCTCCGTG
TTCACTCGCA
GAATATCACT
CAGCAGAATG
GCGTCTGACA
GGTTCTTTGC
CGCCAAAAAA
AGGTCTGCGT
ACTGGTCGCC
GATCTGGGCC
AGCAGATTGT
CGAGATCATC
ATCGTAGGTG
TCTTTGCTCA
GTTGAAGCTT
GGCATCTTCG
GCCAGGTCGA
AGTTAGATTT
AAAGTTGGGG
GTCAGCCCGA
GTGACAGGGC
GATAAGATTA
CCAAACAGGC
CGTTCTTCTT
CTCCCCAGGG
AGCTGTGCGA
TGAGGTTTTC
GAGCCGCCGG
GATTTGGACA
GCCTAGCGCT
CAGGATTGCT
TTGGCGTCAA
CTCCGGATGC
GTTGATCGGG
TTCTCTTCGA
AAAGCTGATT
GTAAGTCTGA
ACCAGTTGGA
TAAAGAGTTT
GTCGACATCG
CTGTTGTCTG
GGTTCAGGTC
CCCCTCGATG
AGGAAGTGTC
CCCGAAATTT
GATCATATGC
GCCAGCGCGA
GATAGATCAA
CCGCAAGTCA
GCCTTATCAG
TACTGTCTAC
AAGCTTCTTC
CTCAGATGAT
ATATGGTTGG
GTACTTTTCA
TGGTACGCCA
CCTCGTCCAC
GATATTGCCA
AAGATTGGGT
GGCGCTCGTG
CTTTTTATCC
TCCTCCACCA
AAAAGGACTC
CTCCAGCCTA
TGGAAGAAAG
AGTCATCCAC
CTTAGCCATC
TCATTACTAA
AGATCTCCTG
CAGGTAGCAG
ATCCGATTCT
TTCTGCGGGT
ATATCTGCGC
CGTGCTGTTC
TTTTGAGCCG
CGTGGCTTCG
GCCGTCTCCC
CGGAGTCGAA
CAGGAGGGCG
CCAATGAGGT
TCTTCTTTAT
GCTGTGGCGA
TCGGTATTGC
CCAGAACTTT
GAATTTTTTG
CTCGGCACCT
TGTACTCGTC
CGTAATGACG
GCCCAGCCGA
CGCTGTTTGT
GCCGATATCG
AGCCCAATGG
AGTACTTCTT
GTCCATGGTG
GCATCGATGG
GATCCTGCAA
AAAGAACAAG
TAGCTTGTAT
TCTATAGTGT
CACCTAAATC
AAGCTTGCTC
CGACGTCCCC
AGGCAGAATG
GCGGTTCCCT
AAACGAGCAT
TGCTTATATA
GACCTCCCAT
TAGGCACGCC
TACCGCCCAT
TTACGTCAAT
GGAACGCCCA
TTTGCGTCAT
TGCCCCTCCC
CATTGACGTC
AATGGGGATG
TACTTGGCAG
CCATCGCGGG
CCATTTACCG
CCATTGACGT
CAATGGGAGT
ACTGCCAATG
TACCCTGGCG
TACTTCCAAT
AGTAATGTAC
TTGCCAAGTT
ACTATTAATA
GATATTGATG
TACTGCCAAG
TGGGCCATTT
ACCGTCATTG
ACGTCAATAG
GGGGCGTGAG
AACGGATATG
AATGGGCAAT
GAGCCATCCC
ATTGACGTCA
ATGGTGGGTG
GTCCTATTGA
CGTCAATGGG
CATTGAGCCA
GGCGGGCCAT
TTACCGTAAT
TGACGTCAAT
GGGGGAGGCG
CCATATACGT
CAATAGGACC
GCCCATATGA
CGTCAATAGG
AAAGACCATA
TATAGAGACC
ATTGACGTCA
ATGGGGGAGT
GGCTATGGGC
GGTATTAGGA
AGCCCCATAT
ATGGTATATG
GGACCGCCCA
TTGGGAGGGG
CTATCTACGT
CAATAGGAAA
ACCCATATAT
GGAATACTAT
ATGGCATAGG
GCCAATACAT
AGTATTGAAC
CTGGCCAATA
GCCATATTGG
CATAGGGCCA
TATTGGATAT
TGCCTATATA
TTGATCCTGG
CATATAGCCA
ATATGGCCGC
CATTATTGGC
ACCATGCCAA
TTCAATATGG
CGGACTTGGC
ACAGGGCCAA
GACCCCTCCT
CAAACTTGGC
ACCGTGCCAA
GTAGACCCCT
CCCCAGTTGG
CACAGTGCCA
GGTCCGCCAT
ATTGAATTGG
CATGGTGCCA
AGTCCGCCAT
ATTGAATTGG
CTGGGGTCCA
GATCCACCAT
ATTGAATTGG
CACGAGTCCA
TATACGCCAT
ATTGAATTGG
CACAGGTCCA
GATCCACCAT
ATTGAATTGG
CATGAGTCCA
TATACGCCAT
ATTGAATTGG
CGGTCGACTG
GCGTAATAGC
GAAGAGGCCC
GCACCGATCG
CCCTTCCCAA
CAGTTGCGCA
GCCTGAATGG
CGAATGGGAC
GCGCCCTGTA
GCGGCGCATT
AAGCGCGGCG
GGTGTGGTGG
TTACGCGCAG
CGTGACCGCT
ACACTTGCCA
GCGCCCTAGC
GCCCGCTCCT
TTCGCTTTCT
TCCCTTCCTT
TCTCGCCACG
TTCGCCGGCT
TTCCCCGTCA
AGCTCTAAAT
CGGGGGCTCC
CTTTAGGGTT
CCGATTTAGT
GCTTTACGGC
ACCTCGACCC
CAAAAAACTT
GATTAGGGTG
ATGGTTCACG
TAGTGGGCCA
TCGCCCTGAT
AGACGGTTTT
TCGCCCTTTG
ACGTTGGAGT
CCACGTTCTT
TAATAGTGGA
CTCTTGTTCC
AAACTGGAAC
AACACTCAAC
CCTATCTCGG
TCTATTCTTT
TGATTTATAA
GGGATTTTGC
CGATTTCGGC
CTATTGGTTA
AAAAATGAGC
TGATTTAACA
AAAATTTAAC
GCGAATTTTA
ACAAAATATT
AACGCTTACA
ATTTAGGTGG
CACTTTTCGG
GGAAATGTGC
GCGGAACCCC
TATTTGTTTA
TTTTTCTAAA
TACATTCAAA
TATGTATCCG
CTCATGAGAC
AATAACCCTG
ATAAATGCTT
CAATAATATT
GAAAAAGGAA
GAGTATGAGT
ATTCAACATT
TCCGTGTCGC
CCTTATTCCC
TTTTTTGCGG
CATTTTGCCT
TCCTGTTTTT
GCTCACCCAG
AAACGCTGGT
GAAAGTAAAA
GATGCTGAAG
ATCAGTTGGG
TGCACGAGTG
GGTTACATCG
AACTGGATCT
CAACAGCGGT
AAGATCCTTG
AGAGTTTTCG
CCCCGAAGAA
CGTTTTCCAA
TGATGAGCAC
TTTTAAAGTT
CTGCTATGTG
GCGCGGTATT
ATCCCGTATT
GACGCCGGGC
AAGAGCAACT
CGGTCGCCGC
ATACACTATT
CTCAGAATGA
CTTGGTTGAG
TACTCACCAG
TCACAGAAAA
GCATCTTACG
GATGGCATGA
CAGTAAGAGA
ATTATGCAGT
GCTGCCATAA
CCATGAGTGA
TAACACTGCG
GCCAACTTAC
TTCTGACAAC
GATCGGAGGA
CCGAAGGAGC
TAACCGCTTT
TTTGCACAAC
ATGGGGGATC
ATGTAACTCG
CCTTGATCGT
TGGGAACCGG
AGCTGAATGA
AGCCATACCA
AACGACGAGC
GTGACACCAC
GATGCCTGTA
GCAATGGCAA
CAACGTTGCG
CAAACTATTA
ACTGGCGAAC
TACTTACTCT
AGCTTCCCGG
CAACAATTAA
TAGACTGGAT
GGAGGCGGAT
AAAGTTGCAG
GACCACTTCT
GCGCTCGGCC
CTTCCGGCTG
GCTGGTTTAT
TGCTGATAAA
TCTGGAGCCG
GTGAGCGTGG
GTCTCGCGGT
ATCATTGCAG
CACTGGGGCC
AGATGGTAAG
CCCTCCCGTA
TCGTAGTTAT
CTACACGACG
GGGAGTCAGG
CAACTATGGA
TGAACGAAAT
AGACAGATCG
CTGAGATAGG
TGCCTCACTG
ATTAAGCATT
GGTAACTGTC
AGACCAAGTT
TACTCATATA
TACTTTAGAT
TGATTTAAAA
CTTCATTTTT
AATTTAAAAG
GATCTAGGTG
AAGATCCTTT
TTGATAATCT
CATGACCAAA
ATCCCTTAAC
GTGAGTTTTC
GTTCCACTGA
GCGTCAGACC
CCGTAGAAAA
GATCAAAGGA
TCTTCTTGAG
ATCCTTTTTT
TCTGCGCGTA
ATCTGCTGCT
TGCAAACAAA
AAAACCACCG
CTACCAGCGG
TGGTTTGTTT
GCCGGATCAA
GAGCTACCAA
CTCTTTTTCC
GAAGGTAACT
GGCTTCAGCA
GAGCGCAGAT
ACCAAATACT
GTTCTTCTAG
TGTAGCCGTA
GTTAGGCCAC
CACTTCAAGA
ACTCTGTAGC
ACCGCCTACA
TACCTCGCTC
TGCTAATCCT
GTTACCAGTG
GCTGCTGCCA
GTGGCGATAA
GTCGTGTCTT
ACCGGGTTGG
ACTCAAGACG
ATAGTTACCG
GATAAGGCGC
AGCGGTCGGG
CTGAACGGGG
GGTTCGTGCA
CACAGCCCAG
CTTGGAGCGA
ACGACCTACA
CCGAACTGAG
ATACCTACAG
CGTGAGCTAT
GAGAAAGCGC
CACGCTTCCC
GAAGGGAGAA
AGGCGGACAG
GTATCCGGTA
AGCGGCAGGG
TCGGAACAGG
AGAGCGCACG
AGGGAGCTTC
CAGGGGGAAA
CGCCTGGTAT
CTTTATAGTC
CTGTCGGGTT
TCGCCACCTC
TGACTTGAGC
GTCGATTTTT
GTGATGCTCG
TCAGGGGGGC
GGAGCCTATG
GAAAAACGCC
AGCAACGCGG
CCTTTTTACG
GTTCCTGGCC
TTTTGCTGGC
CTTTTGCTCA
CATGTTCTTT
CCTGCGTTAT
CCCCTGATTC
TGTGGATAAC
CGTATTACCG
CCTTTGAGTG
AGCTGATACC
GCTCGCCGCA
GCCGAACGAC
CGAGCGCAGC
GAGTCAGTGA
GCGAGGAAGC
GGAAG