名称: | pET28b-T7-Cas9-6×His |
---|---|
别称: | |
基因长: | |
质粒宿主: | 大肠杆菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR,Cas9 |
片段物种: | |
原核抗性: | Kan |
筛选标记: | |
荧光标记: | |
启动子: | T7 |
复制子: | pBR322 |
拷贝数: | 低 |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | T7:TAATACGACTCACTATAGGG |
反向引物: | T7ter:TGCTAGTTATTGCTCAGCGG |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AGCGCCTGAT
GCGGTATTTT
CTCCTTACGC
ATCTGTGCGG
TATTTCACAC
CGCAATGGTG
CACTCTCAGT
ACAATCTGCT
CTGATGCCGC
ATAGTTAAGC
CAGTATACAC
TCCGCTATCG
CTACGTGACT
GGGTCATGGC
TGCGCCCCGA
CACCCGCCAA
CACCCGCTGA
CGCGCCCTGA
CGGGCTTGTC
TGCTCCCGGC
ATCCGCTTAC
AGACAAGCTG
TGACCGTCTC
CGGGAGCTGC
ATGTGTCAGA
GGTTTTCACC
GTCATCACCG
AAACGCGCGA
GGCAGCTGCG
GTAAAGCTCA
TCAGCGTGGT
CGTGAAGCGA
TTCACAGATG
TCTGCCTGTT
CATCCGCGTC
CAGCTCGTTG
AGTTTCTCCA
GAAGCGTTAA
TGTCTGGCTT
CTGATAAAGC
GGGCCATGTT
AAGGGCGGTT
TTTTCCTGTT
TGGTCACTGA
TGCCTCCGTG
TAAGGGGGAT
TTCTGTTCAT
GGGGGTAATG
ATACCGATGA
AACGAGAGAG
GATGCTCACG
ATACGGGTTA
CTGATGATGA
ACATGCCCGG
TTACTGGAAC
GTTGTGAGGG
TAAACAACTG
GCGGTATGGA
TGCGGCGGGA
CCAGAGAAAA
ATCACTCAGG
GTCAATGCCA
GCGCTTCGTT
AATACAGATG
TAGGTGTTCC
ACAGGGTAGC
CAGCAGCATC
CTGCGATGCA
GATCCGGAAC
ATAATGGTGC
AGGGCGCTGA
CTTCCGCGTT
TCCAGACTTT
ACGAAACACG
GAAACCGAAG
ACCATTCATG
TTGTTGCTCA
GGTCGCAGAC
GTTTTGCAGC
AGCAGTCGCT
TCACGTTCGC
TCGCGTATCG
GTGATTCATT
CTGCTAACCA
GTAAGGCAAC
CCCGCCAGCC
TAGCCGGGTC
CTCAACGACA
GGAGCACGAT
CATGCGCACC
CGTGGGGCCG
CCATGCCGGC
GATAATGGCC
TGCTTCTCGC
CGAAACGTTT
GGTGGCGGGA
CCAGTGACGA
AGGCTTGAGC
GAGGGCGTGC
AAGATTCCGA
ATACCGCAAG
CGACAGGCCG
ATCATCGTCG
CGCTCCAGCG
AAAGCGGTCC
TCGCCGAAAA
TGACCCAGAG
CGCTGCCGGC
ACCTGTCCTA
CGAGTTGCAT
GATAAAGAAG
ACAGTCATAA
GTGCGGCGAC
GATAGTCATG
CCCCGCGCCC
ACCGGAAGGA
GCTGACTGGG
TTGAAGGCTC
TCAAGGGCAT
CGGTCGAGAT
CCCGGTGCCT
AATGAGTGAG
CTAACTTACA
TTAATTGCGT
TGCGCTCACT
GCCCGCTTTC
CAGTCGGGAA
ACCTGTCGTG
CCAGCTGCAT
TAATGAATCG
GCCAACGCGC
GGGGAGAGGC
GGTTTGCGTA
TTGGGCGCCA
GGGTGGTTTT
TCTTTTCACC
AGTGAGACGG
GCAACAGCTG
ATTGCCCTTC
ACCGCCTGGC
CCTGAGAGAG
TTGCAGCAAG
CGGTCCACGC
TGGTTTGCCC
CAGCAGGCGA
AAATCCTGTT
TGATGGTGGT
TAACGGCGGG
ATATAACATG
AGCTGTCTTC
GGTATCGTCG
TATCCCACTA
CCGAGATATC
CGCACCAACG
CGCAGCCCGG
ACTCGGTAAT
GGCGCGCATT
GCGCCCAGCG
CCATCTGATC
GTTGGCAACC
AGCATCGCAG
TGGGAACGAT
GCCCTCATTC
AGCATTTGCA
TGGTTTGTTG
AAAACCGGAC
ATGGCACTCC
AGTCGCCTTC
CCGTTCCGCT
ATCGGCTGAA
TTTGATTGCG
AGTGAGATAT
TTATGCCAGC
CAGCCAGACG
CAGACGCGCC
GAGACAGAAC
TTAATGGGCC
CGCTAACAGC
GCGATTTGCT
GGTGACCCAA
TGCGACCAGA
TGCTCCACGC
CCAGTCGCGT
ACCGTCTTCA
TGGGAGAAAA
TAATACTGTT
GATGGGTGTC
TGGTCAGAGA
CATCAAGAAA
TAACGCCGGA
ACATTAGTGC
AGGCAGCTTC
CACAGCAATG
GCATCCTGGT
CATCCAGCGG
ATAGTTAATG
ATCAGCCCAC
TGACGCGTTG
CGCGAGAAGA
TTGTGCACCG
CCGCTTTACA
GGCTTCGACG
CCGCTTCGTT
CTACCATCGA
CACCACCACG
CTGGCACCCA
GTTGATCGGC
GCGAGATTTA
ATCGCCGCGA
CAATTTGCGA
CGGCGCGTGC
AGGGCCAGAC
TGGAGGTGGC
AACGCCAATC
AGCAACGACT
GTTTGCCCGC
CAGTTGTTGT
GCCACGCGGT
TGGGAATGTA
ATTCAGCTCC
GCCATCGCCG
CTTCCACTTT
TTCCCGCGTT
TTCGCAGAAA
CGTGGCTGGC
CTGGTTCACC
ACGCGGGAAA
CGGTCTGATA
AGAGACACCG
GCATACTCTG
CGACATCGTA
TAACGTTACT
GGTTTCACAT
TCACCACCCT
GAATTGACTC
TCTTCCGGGC
GCTATCATGC
CATACCGCGA
AAGGTTTTGC
GCCATTCGAT
GGTGTCCGGG
ATCTCGACGC
TCTCCCTTAT
GCGACTCCTG
CATTAGGAAG
CAGCCCAGTA
GTAGGTTGAG
GCCGTTGAGC
ACCGCCGCCG
CAAGGAATGG
TGCATGCAAG
GAGATGGCGC
CCAACAGTCC
CCCGGCCACG
GGGCCTGCCA
CCATACCCAC
GCCGAAACAA
GCGCTCATGA
GCCCGAAGTG
GCGAGCCCGA
TCTTCCCCAT
CGGTGATGTC
GGCGATATAG
GCGCCAGCAA
CCGCACCTGT
GGCGCCGGTG
ATGCCGGCCA
CGATGCGTCC
GGCGTAGAGG
ATCGAGATCT
CGATCCCGCG
AAATTAATAC
GACTCACTAT
AGGGGAATTG
TGAGCGGATA
ACAATTCCCC
TCTAGAAATA
ATTTTGTTTA
ACTTTAAGAA
GGAGATATAC
CATGGACAAG
AAGTACTCCA
TTGGGCTCGA
TATCGGCACA
AACAGCGTCG
GCTGGGCCGT
CATTACGGAC
GAGTACAAGG
TGCCGAGCAA
AAAATTCAAA
GTTCTGGGCA
ATACCGATCG
CCACAGCATA
AAGAAGAACC
TCATTGGCGC
CCTCCTGTTC
GACTCCGGGG
AGACGGCCGA
AGCCACGCGG
CTCAAAAGAA
CAGCACGGCG
CAGATATACC
CGCAGAAAGA
ATCGGATCTG
CTACCTGCAG
GAGATCTTTA
GTAATGAGAT
GGCTAAGGTG
GATGACTCTT
TCTTCCATAG
GCTGGAGGAG
TCCTTTTTGG
TGGAGGAGGA
TAAAAAGCAC
GAGCGCCACC
CAATCTTTGG
CAATATCGTG
GACGAGGTGG
CGTACCATGA
AAAGTACCCA
ACCATATATC
ATCTGAGGAA
GAAGCTTGTA
GACAGTACTG
ATAAGGCTGA
CTTGCGGTTG
ATCTATCTCG
CGCTGGCGCA
TATGATCAAA
TTTCGGGGAC
ACTTCCTCAT
CGAGGGGGAC
CTGAACCCAG
ACAACAGCGA
TGTCGACAAA
CTCTTTATCC
AACTGGTTCA
GACTTACAAT
CAGCTTTTCG
AAGAGAACCC
GATCAACGCA
TCCGGAGTTG
ACGCCAAAGC
AATCCTGAGC
GCTAGGCTGT
CCAAATCCCG
GCGGCTCGAA
AACCTCATCG
CACAGCTCCC
TGGGGAGAAG
AAGAACGGCC
TGTTTGGTAA
TCTTATCGCC
CTGTCACTCG
GGCTGACCCC
CAACTTTAAA
TCTAACTTCG
ACCTGGCCGA
AGATGCCAAG
CTTCAACTGA
GCAAAGACAC
CTACGATGAT
GATCTCGACA
ATCTGCTGGC
CCAGATCGGC
GACCAGTACG
CAGACCTTTT
TTTGGCGGCA
AAGAACCTGT
CAGACGCCAT
TCTGCTGAGT
GATATTCTGC
GAGTGAACAC
GGAGATCACC
AAAGCTCCGC
TGAGCGCTAG
TATGATCAAG
CGCTATGATG
AGCACCACCA
AGACTTGACT
TTGCTGAAGG
CCCTTGTCAG
ACAGCAACTG
CCTGAGAAGT
ACAAGGAAAT
TTTCTTCGAT
CAGTCTAAAA
ATGGCTACGC
CGGATACATT
GACGGCGGAG
CAAGCCAGGA
GGAATTTTAC
AAATTTATTA
AGCCCATCTT
GGAAAAAATG
GACGGCACCG
AGGAGCTGCT
GGTAAAGCTT
AACAGAGAAG
ATCTGTTGCG
CAAACAGCGC
ACTTTCGACA
ATGGAAGCAT
CCCCCACCAG
ATTCACCTGG
GCGAACTGCA
CGCTATCCTC
AGGCGGCAAG
AGGATTTCTA
CCCCTTTTTG
AAAGATAACA
GGGAAAAGAT
TGAGAAAATC
CTCACATTTC
GGATACCCTA
CTATGTAGGC
CCCCTCGCCC
GGGGAAATTC
CAGATTCGCG
TGGATGACTC
GCAAATCAGA
AGAGACCATC
ACTCCCTGGA
ACTTCGAGGA
AGTCGTGGAT
AAGGGGGCCT
CTGCCCAGTC
CTTCATCGAA
AGGATGACTA
ACTTTGATAA
AAATCTGCCT
AACGAAAAGG
TGCTTCCTAA
ACACTCTCTG
CTGTACGAGT
ACTTCACAGT
TTATAACGAG
CTCACCAAGG
TCAAATACGT
CACAGAAGGG
ATGAGAAAGC
CAGCATTCCT
GTCTGGAGAG
CAGAAGAAAG
CTATCGTGGA
CCTCCTCTTC
AAGACGAACC
GGAAAGTTAC
CGTGAAACAG
CTCAAAGAAG
ACTATTTCAA
AAAGATTGAA
TGTTTCGACT
CTGTTGAAAT
CAGCGGAGTG
GAGGATCGCT
TCAACGCATC
CCTGGGAACG
TATCACGATC
TCCTGAAAAT
CATTAAAGAC
AAGGACTTCC
TGGACAATGA
GGAGAACGAG
GACATTCTTG
AGGACATTGT
CCTCACCCTT
ACGTTGTTTG
AAGATAGGGA
GATGATTGAA
GAACGCTTGA
AAACTTACGC
TCATCTCTTC
GACGACAAAG
TCATGAAACA
GCTCAAGAGG
CGCCGATATA
CAGGATGGGG
GCGGCTGTCA
AGAAAACTGA
TCAATGGGAT
CCGAGACAAG
CAGAGTGGAA
AGACAATCCT
GGATTTTCTT
AAGTCCGATG
GATTTGCCAA
CCGGAACTTC
ATGCAGTTGA
TCCATGATGA
CTCTCTCACC
TTTAAGGAGG
ACATCCAGAA
AGCACAAGTT
TCTGGCCAGG
GGGACAGTCT
TCACGAGCAC
ATCGCTAATC
TTGCAGGTAG
CCCAGCTATC
AAAAAGGGAA
TACTGCAGAC
CGTTAAGGTC
GTGGATGAAC
TCGTCAAAGT
AATGGGAAGG
CATAAGCCCG
AGAATATCGT
TATCGAGATG
GCCCGAGAGA
ACCAAACTAC
CCAGAAGGGA
CAGAAGAACA
GTAGGGAAAG
GATGAAGAGG
ATTGAAGAGG
GTATAAAAGA
ACTGGGGTCC
CAAATCCTTA
AGGAACACCC
AGTTGAAAAC
ACCCAGCTTC
AGAATGAGAA
GCTCTACCTG
TACTACCTGC
AGAACGGCAG
GGACATGTAC
GTGGATCAGG
AACTGGACAT
CAATCGGCTC
TCCGACTACG
ACGTGGATCA
TATCGTGCCC
CAGTCTTTTC
TCAAAGATGA
TTCTATTGAT
AATAAAGTGT
TGACAAGATC
CGATAAAAAT
AGAGGGAAGA
GTGATAACGT
CCCCTCAGAA
GAAGTTGTCA
AGAAAATGAA
AAATTATTGG
CGGCAGCTGC
TGAACGCCAA
ACTGATCACA
CAACGGAAGT
TCGATAATCT
GACTAAGGCT
GAACGAGGTG
GCCTGTCTGA
GTTGGATAAA
GCCGGCTTCA
TCAAAAGGCA
GCTTGTTGAG
ACACGCCAGA
TCACCAAGCA
CGTGGCCCAA
ATTCTCGATT
CACGCATGAA
CACCAAGTAC
GATGAAAATG
ACAAACTGAT
TCGAGAGGTG
AAAGTTATTA
CTCTGAAGTC
TAAGCTGGTC
TCAGATTTCA
GAAAGGACTT
TCAGTTTTAT
AAGGTGAGAG
AGATCAACAA
TTACCACCAT
GCGCATGATG
CCTACCTGAA
TGCAGTGGTA
GGCACTGCAC
TTATCAAAAA
ATATCCCAAG
CTTGAATCTG
AATTTGTTTA
CGGAGACTAT
AAAGTGTACG
ATGTTAGGAA
AATGATCGCA
AAGTCTGAGC
AGGAAATAGG
CAAGGCCACC
GCTAAGTACT
TCTTTTACAG
CAATATTATG
AATTTTTTCA
AGACCGAGAT
TACACTGGCC
AATGGAGAGA
TTCGGAAGCG
ACCACTTATC
GAAACAAACG
GAGAAACAGG
AGAAATCGTG
TGGGACAAGG
GTAGGGATTT
CGCGACAGTC
CGGAAGGTCC
TGTCCATGCC
GCAGGTGAAC
ATCGTTAAAA
AGACCGAAGT
ACAGACCGGA
GGCTTCTCCA
AGGAAAGTAT
CCTCCCGAAA
AGGAACAGCG
ACAAGCTGAT
CGCACGCAAA
AAAGATTGGG
ACCCCAAGAA
ATACGGCGGA
TTCGATTCTC
CTACAGTCGC
TTACAGTGTA
CTGGTTGTGG
CCAAAGTGGA
GAAAGGGAAG
TCTAAAAAAC
TCAAAAGCGT
CAAGGAACTG
CTGGGCATCA
CAATCATGGA
GCGATCAAGC
TTCGAAAAAA
ACCCCATCGA
CTTTCTCGAG
GCGAAAGGAT
ATAAAGAGGT
CAAAAAAGAC
CTCATCATTA
AGCTTCCCAA
GTACTCTCTC
TTTGAGCTTG
AAAACGGCCG
GAAACGAATG
CTCGCTAGTG
CGGGCGAGCT
GCAGAAAGGT
AACGAGCTGG
CACTGCCCTC
TAAATACGTT
AATTTCTTGT
ATCTGGCCAG
CCACTATGAA
AAGCTCAAAG
GGTCTCCCGA
AGATAATGAG
CAGAAGCAGC
TGTTCGTGGA
ACAACACAAA
CACTACCTTG
ATGAGATCAT
CGAGCAAATA
AGCGAATTCT
CCAAAAGAGT
GATCCTCGCC
GACGCTAACC
TCGATAAGGT
GCTTTCTGCT
TACAATAAGC
ACAGGGATAA
GCCCATCAGG
GAGCAGGCAG
AAAACATTAT
CCACTTGTTT
ACTCTGACCA
ACTTGGGCGC
GCCTGCAGCC
TTCAAGTACT
TCGACACCAC
CATAGACAGA
AAGCGGTACA
CCTCTACAAA
GGAGGTCCTG
GACGCCACAC
TGATTCATCA
GTCAATTACG
GGGCTCTATG
AAACAAGAAT
CGACCTCTCT
CAGCTCGGTG
GAGACAGCAG
GGCTGACGCG
GCCGCACTCG
AGCACCACCA
CCACCACCAC
TGAGATCCGG
CTGCTAACAA
AGCCCGAAAG
GAAGCTGAGT
TGGCTGCTGC
CACCGCTGAG
CAATAACTAG
CATAACCCCT
TGGGGCCTCT
AAACGGGTCT
TGAGGGGTTT
TTTGCTGAAA
GGAGGAACTA
TATCCGGATT
GGCGAATGGG
ACGCGCCCTG
TAGCGGCGCA
TTAAGCGCGG
CGGGTGTGGT
GGTTACGCGC
AGCGTGACCG
CTACACTTGC
CAGCGCCCTA
GCGCCCGCTC
CTTTCGCTTT
CTTCCCTTCC
TTTCTCGCCA
CGTTCGCCGG
CTTTCCCCGT
CAAGCTCTAA
ATCGGGGGCT
CCCTTTAGGG
TTCCGATTTA
GTGCTTTACG
GCACCTCGAC
CCCAAAAAAC
TTGATTAGGG
TGATGGTTCA
CGTAGTGGGC
CATCGCCCTG
ATAGACGGTT
TTTCGCCCTT
TGACGTTGGA
GTCCACGTTC
TTTAATAGTG
GACTCTTGTT
CCAAACTGGA
ACAACACTCA
ACCCTATCTC
GGTCTATTCT
TTTGATTTAT
AAGGGATTTT
GCCGATTTCG
GCCTATTGGT
TAAAAAATGA
GCTGATTTAA
CAAAAATTTA
ACGCGAATTT
TAACAAAATA
TTAACGCTTA
CAATTTAGGT
GGCACTTTTC
GGGGAAATGT
GCGCGGAACC
CCTATTTGTT
TATTTTTCTA
AATACATTCA
AATATGTATC
CGCTCATGAA
TTAATTCTTA
GAAAAACTCA
TCGAGCATCA
AATGAAACTG
CAATTTATTC
ATATCAGGAT
TATCAATACC
ATATTTTTGA
AAAAGCCGTT
TCTGTAATGA
AGGAGAAAAC
TCACCGAGGC
AGTTCCATAG
GATGGCAAGA
TCCTGGTATC
GGTCTGCGAT
TCCGACTCGT
CCAACATCAA
TACAACCTAT
TAATTTCCCC
TCGTCAAAAA
TAAGGTTATC
AAGTGAGAAA
TCACCATGAG
TGACGACTGA
ATCCGGTGAG
AATGGCAAAA
GTTTATGCAT
TTCTTTCCAG
ACTTGTTCAA
CAGGCCAGCC
ATTACGCTCG
TCATCAAAAT
CACTCGCATC
AACCAAACCG
TTATTCATTC
GTGATTGCGC
CTGAGCGAGA
CGAAATACGC
GATCGCTGTT
AAAAGGACAA
TTACAAACAG
GAATCGAATG
CAACCGGCGC
AGGAACACTG
CCAGCGCATC
AACAATATTT
TCACCTGAAT
CAGGATATTC
TTCTAATACC
TGGAATGCTG
TTTTCCCGGG
GATCGCAGTG
GTGAGTAACC
ATGCATCATC
AGGAGTACGG
ATAAAATGCT
TGATGGTCGG
AAGAGGCATA
AATTCCGTCA
GCCAGTTTAG
TCTGACCATC
TCATCTGTAA
CATCATTGGC
AACGCTACCT
TTGCCATGTT
TCAGAAACAA
CTCTGGCGCA
TCGGGCTTCC
CATACAATCG
ATAGATTGTC
GCACCTGATT
GCCCGACATT
ATCGCGAGCC
CATTTATACC
CATATAAATC
AGCATCCATG
TTGGAATTTA
ATCGCGGCCT
AGAGCAAGAC
GTTTCCCGTT
GAATATGGCT
CATAACACCC
CTTGTATTAC
TGTTTATGTA
AGCAGACAGT
TTTATTGTTC
ATGACCAAAA
TCCCTTAACG
TGAGTTTTCG
TTCCACTGAG
CGTCAGACCC
CGTAGAAAAG
ATCAAAGGAT
CTTCTTGAGA
TCCTTTTTTT
CTGCGCGTAA
TCTGCTGCTT
GCAAACAAAA
AAACCACCGC
TACCAGCGGT
GGTTTGTTTG
CCGGATCAAG
AGCTACCAAC
TCTTTTTCCG
AAGGTAACTG
GCTTCAGCAG
AGCGCAGATA
CCAAATACTG
TCCTTCTAGT
GTAGCCGTAG
TTAGGCCACC
ACTTCAAGAA
CTCTGTAGCA
CCGCCTACAT
ACCTCGCTCT
GCTAATCCTG
TTACCAGTGG
CTGCTGCCAG
TGGCGATAAG
TCGTGTCTTA
CCGGGTTGGA
CTCAAGACGA
TAGTTACCGG
ATAAGGCGCA
GCGGTCGGGC
TGAACGGGGG
GTTCGTGCAC
ACAGCCCAGC
TTGGAGCGAA
CGACCTACAC
CGAACTGAGA
TACCTACAGC
GTGAGCTATG
AGAAAGCGCC
ACGCTTCCCG
AAGGGAGAAA
GGCGGACAGG
TATCCGGTAA
GCGGCAGGGT
CGGAACAGGA
GAGCGCACGA
GGGAGCTTCC
AGGGGGAAAC
GCCTGGTATC
TTTATAGTCC
TGTCGGGTTT
CGCCACCTCT
GACTTGAGCG
TCGATTTTTG
TGATGCTCGT
CAGGGGGGCG
GAGCCTATGG
AAAAACGCCA
GCAACGCGGC
CTTTTTACGG
TTCCTGGCCT
TTTGCTGGCC
TTTTGCTCAC
ATGTTCTTTC
CTGCGTTATC
CCCTGATTCT
GTGGATAACC
GTATTACCGC
CTTTGAGTGA
GCTGATACCG
CTCGCCGCAG
CCGAACGACC
GAGCGCAGCG
AGTCAGTGAG
CGAGGAAGCG
GAAG