名称: | pAAVS1-NDi-CRISPRi(Gen2) |
---|---|
别称: | Plasmid#73498 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因敲入 |
片段类型: | CRISPR,ORF |
片段物种: | |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | TRE3G |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AAGGGCGAAT
TCAACCCTGA
TAAATGCTTC
AATAATATTG
AAAAAGGAAG
AGTATGAGTA
TTCAACATTT
CCGTGTCGCC
CTTATTCCCT
TTTTTGCGGC
ATTTTGCCTT
CCTGTTTTTG
CTCACCCAGA
AACGCTGGTG
AAAGTAAAAG
ATGCTGAAGA
TCAGTTGGGT
GCACGAGTGG
GTTACATCGA
ACTGGATCTC
AACAGCGGTA
AGATCCTTGA
GAGTTTTCGC
CCCGAAGAAC
GTTTTCCAAT
GATGAGCACT
TTTAAAGTTC
TGCTATGTGG
CGCGGTATTA
TCCCGTATTG
ACGCCGGGCA
AGAGCAACTC
GGTCGCCGCA
TACACTATTC
TCAGAATGAC
TTGGTTGAGT
ACTCACCAGT
CACAGAAAAG
CATCTTACGG
ATGGCATGAC
AGTAAGAGAA
TTATGCAGTG
CTGCCATAAC
CATGAGTGAT
AACACTGCGG
CCAACTTACT
TCTGACAACG
ATCGGAGGAC
CGAAGGAGCT
AACCGCTTTT
TTGCACAACA
TGGGGGATCA
TGTAACTCGC
CTTGATCGTT
GGGAACCGGA
GCTGAATGAA
GCCATACCAA
ACGACGAGCG
TGACACCACG
ATGCCTGTAG
CAATGGCAAC
AACGTTGCGC
AAACTATTAA
CTGGCGAACT
ACTTACTCTA
GCTTCCCGGC
AACAATTAAT
AGACTGGATG
GAGGCGGATA
AAGTTGCAGG
ACCACTTCTG
CGCTCGGCCC
TTCCGGCTGG
CTGGTTTATT
GCTGATAAAT
CTGGAGCCGG
TGAGCGTGGG
TCTCGCGGTA
TCATTGCAGC
ACTGGGGCCA
GATGGTAAGC
CCTCCCGTAT
CGTAGTTATC
TACACGACGG
GGAGTCAGGC
AACTATGGAT
GAACGAAATA
GACAGATCGC
TGAGATAGGT
GCCTCACTGA
TTAAGCATTG
GTAACTGTCA
GACCAAGTTT
ACTCATATAT
ACTTTAGATT
GATTTAAAAC
TTCATTTTTA
ATTTAAAAGG
ATCTAGGTGA
AGATCCTTTT
TGATAATCTC
ATGACCAAAA
TCCCTTAACG
TGAGTTTTCG
TTCCACTGAG
CGTCAGACCC
CGTAGAAAAG
ATCAAAGGAT
CTTCTTGAGA
TCCTTTTTTT
CTGCGCGTAA
TCTGCTGCTT
GCAAACAAAA
AAACCACCGC
TACCAGCGGT
GGTTTGTTTG
CCGGATCAAG
AGCTACCAAC
TCTTTTTCCG
AAGGTAACTG
GCTTCAGCAG
AGCGCAGATA
CCAAATACTG
TCCTTCTAGT
GTAGCCGTAG
TTAGGCCACC
ACTTCAAGAA
CTCTGTAGCA
CCGCCTACAT
ACCTCGCTCT
GCTAATCCTG
TTACCAGTGG
CTGCTGCCAG
TGGCGATAAG
TCGTGTCTTA
CCGGGTTGGA
CTCAAGACGA
TAGTTACCGG
ATAAGGCGCA
GCGGTCGGGC
TGAACGGGGG
GTTCGTGCAC
ACAGCCCAGC
TTGGAGCGAA
CGACCTACAC
CGAACTGAGA
TACCTACAGC
GTGAGCTATG
AGAAAGCGCC
ACGCTTCCCG
AAGGGAGAAA
GGCGGACAGG
TATCCGGTAA
GCGGCAGGGT
CGGAACAGGA
GAGCGCACGA
GGGAGCTTCC
AGGGGGAAAC
GCCTGGTATC
TTTATAGTCC
TGTCGGGTTT
CGCCACCTCT
GACTTGAGCG
TCGATTTTTG
TGATGCTCGT
CAGGGGGGCG
GAGCCTATGG
AAAAACGCCA
GCAACGCGGC
CTTTTTACGG
TTCCTGGCGA
ATTCGCCCTT
AGAGCAGAGC
CAGGAACCCC
TGTAGGGAAG
GGGCAGGAGA
GCCAGGGGCA
TGAGATGGTG
GACGAGGAAG
GGGGACAGGG
AAGCCTGAGC
GCCTCTCCTG
GGCTTGCCAA
GGACTCAAAC
CCAGAAGCCC
AGAGCAGGGC
CTTAGGGAAG
CGGGACCCTG
CTCTGGGCGG
AGGAATATGT
CCCAGATAGC
ACTGGGGACT
CTTTAAGGAA
AGAAGGATGG
AGAAAGAGAA
AGGGAGTAGA
GGCGGCCACG
ACCTGGTGAA
CACCTAGGAC
GCACCATTCT
CACAAAGGGA
GTTTTCCACA
CGGACACCCC
CCTCCTCACC
ACAGCCCTGC
CAGGACGGGG
CTGGCTACTG
GCCTTATCTC
ACAGGTAAAA
CTGACGCACG
GAGGAACAAT
ATAAATTGGG
GACTAGAAAG
GTGAAGAGCC
AAAGTTAGAA
CTCAGGACCA
ACTTATTCTG
ATTTTGTTTT
TCCAAACTGC
TTCTCCTCTT
GGGAAGTGTA
AGGAAGCTGC
AGCACCAGGA
TCAGTGAAAC
GCACCAGACA
GCCGCGTCAG
AGCAGCTCAG
GTTCTGGGAG
AGGGTAGCGC
AGGGTGGCCA
CTGAGAACCG
GGCAGGTCAC
GCATCCCCCC
CTTCCCTCCC
ACCCCCTGCC
AAGCTCTCCC
TCCCAGGATC
CTCTCTGGCT
CCATCGTAAG
CAAACCTTAG
AGGTTCTGGC
AAGGAGAGAG
ATGGCTCCAG
GAAATGGGGG
TGTGTCACCA
GATAAGGAAT
CTGCCTAACA
GGAGGTGGGG
GTTAGACCCA
ATATCAGGAG
ACTAGGAAGG
AGGAGGCCTA
AGGATGGGGC
TTTTCTGTCA
CCAATCCTGT
CCCTAGTAAA
GCTGATATCT
CCACCCCACA
GTGGTTTACT
CCCTATCAGT
GATAGAGAAC
GTATGAAGAG
TTTACTCCCT
ATCAGTGATA
GAGAACGTAT
GCAGACTTTA
CTCCCTATCA
GTGATAGAGA
ACGTATAAGG
AGTTTACTCC
CTATCAGTGA
TAGAGAACGT
ATGACCAGTT
TACTCCCTAT
CAGTGATAGA
GAACGTATCT
ACAGTTTACT
CCCTATCAGT
GATAGAGAAC
GTATATCCAG
TTTACTCCCT
ATCAGTGATA
GAGAACGTAT
AAGCTTTAGG
CGTGTACGGT
GGGCGCCTAT
AAAAGCAGAG
CTCGTTTAGT
GAACCGTCAG
ATCGCCTGGA
GCAATTCCAC
AACACTTTTG
TCTTATACCA
ACTTTCCGTA
CCACTTCCTA
CCCTCGTAAA
CTTAAGGTTA
ATTAACGCCA
CCATGGATGC
TAAGTCACTA
ACTGCCTGGT
CCCGGACACT
GGTGACCTTC
AAGGATGTAT
TTGTGGACTT
CACCAGGGAG
GAGTGGAAGC
TGCTGGACAC
TGCTCAGCAG
ATCGTGTACA
GAAATGTGAT
GCTGGAGAAC
TATAAGAACC
TGGTTTCCTT
GGGTTATCAG
CTTACTAAGC
CAGATGTGAT
CCTCCGGTTG
GAGAAGGGAG
AAGAGCCCGG
CGGTTCCGGC
GGAGGGTCGA
TGGACAAGAA
GTACAGCATC
GGCCTGGCCA
TCGGCACCAA
CTCTGTGGGC
TGGGCCGTGA
TCACCGACGA
GTACAAGGTG
CCCAGCAAGA
AATTCAAGGT
GCTGGGCAAC
ACCGACCGGC
ACAGCATCAA
GAAGAACCTG
ATCGGCGCCC
TGCTGTTCGA
CAGCGGAGAA
ACAGCCGAGG
CCACCCGGCT
GAAGAGAACC
GCCAGAAGAA
GATACACCAG
ACGGAAGAAC
CGGATCTGCT
ATCTGCAAGA
GATCTTCAGC
AACGAGATGG
CCAAGGTGGA
CGACAGCTTC
TTCCACAGAC
TGGAAGAGTC
CTTCCTGGTG
GAAGAGGATA
AGAAGCACGA
GCGGCACCCC
ATCTTCGGCA
ACATCGTGGA
CGAGGTGGCC
TACCACGAGA
AGTACCCCAC
CATCTACCAC
CTGAGAAAGA
AACTGGTGGA
CAGCACCGAC
AAGGCCGACC
TGCGGCTGAT
CTATCTGGCC
CTGGCCCACA
TGATCAAGTT
CCGGGGCCAC
TTCCTGATCG
AGGGCGACCT
GAACCCCGAC
AACAGCGACG
TGGACAAGCT
GTTCATCCAG
CTGGTGCAGA
CCTACAACCA
GCTGTTCGAG
GAAAACCCCA
TCAACGCCAG
CGGCGTGGAC
GCCAAGGCCA
TCCTGTCTGC
CAGACTGAGC
AAGAGCAGAC
GGCTGGAAAA
TCTGATCGCC
CAGCTGCCCG
GCGAGAAGAA
GAATGGCCTG
TTCGGCAACC
TGATTGCCCT
GAGCCTGGGC
CTGACCCCCA
ACTTCAAGAG
CAACTTCGAC
CTGGCCGAGG
ATGCCAAACT
GCAGCTGAGC
AAGGACACCT
ACGACGACGA
CCTGGACAAC
CTGCTGGCCC
AGATCGGCGA
CCAGTACGCC
GACCTGTTTC
TGGCCGCCAA
GAACCTGTCC
GACGCCATCC
TGCTGAGCGA
CATCCTGAGA
GTGAACACCG
AGATCACCAA
GGCCCCCCTG
AGCGCCTCTA
TGATCAAGAG
ATACGACGAG
CACCACCAGG
ACCTGACCCT
GCTGAAAGCT
CTCGTGCGGC
AGCAGCTGCC
TGAGAAGTAC
AAAGAGATTT
TCTTCGACCA
GAGCAAGAAC
GGCTACGCCG
GCTACATCGA
TGGCGGAGCC
AGCCAGGAAG
AGTTCTACAA
GTTCATCAAG
CCCATCCTGG
AAAAGATGGA
CGGCACCGAG
GAACTGCTCG
TGAAGCTGAA
CAGAGAGGAC
CTGCTGCGGA
AGCAGCGGAC
CTTCGACAAC
GGCAGCATCC
CCCACCAGAT
CCACCTGGGA
GAGCTGCACG
CCATTCTGCG
GCGGCAGGAA
GATTTTTACC
CATTCCTGAA
GGACAACCGG
GAAAAGATCG
AGAAGATCCT
GACCTTCCGC
ATCCCCTACT
ACGTGGGCCC
TCTGGCCAGG
GGAAACAGCA
GATTCGCCTG
GATGACCAGA
AAGAGCGAGG
AAACCATCAC
CCCCTGGAAC
TTCGAGGAAG
TGGTGGACAA
GGGCGCCAGC
GCCCAGAGCT
TCATCGAGCG
GATGACCAAC
TTCGATAAGA
ACCTGCCCAA
CGAGAAGGTG
CTGCCCAAGC
ACAGCCTGCT
GTACGAGTAC
TTCACCGTGT
ACAACGAGCT
GACCAAAGTG
AAATACGTGA
CCGAGGGAAT
GAGAAAGCCC
GCCTTCCTGA
GCGGCGAGCA
GAAAAAAGCC
ATCGTGGACC
TGCTGTTCAA
GACCAACCGG
AAAGTGACCG
TGAAGCAGCT
GAAAGAGGAC
TACTTCAAGA
AAATCGAGTG
CTTCGACTCC
GTGGAAATCT
CCGGCGTGGA
AGATCGGTTC
AACGCCTCCC
TGGGCACATA
CCACGATCTG
CTGAAAATTA
TCAAGGACAA
GGACTTCCTG
GACAATGAGG
AAAACGAGGA
CATTCTGGAA
GATATCGTGC
TGACCCTGAC
ACTGTTTGAG
GACAGAGAGA
TGATCGAGGA
ACGGCTGAAA
ACCTATGCCC
ACCTGTTCGA
CGACAAAGTG
ATGAAGCAGC
TGAAGCGGCG
GAGATACACC
GGCTGGGGCA
GGCTGAGCCG
GAAGCTGATC
AACGGCATCC
GGGACAAGCA
GTCCGGCAAG
ACAATCCTGG
ATTTCCTGAA
GTCCGACGGC
TTCGCCAACA
GAAACTTCAT
GCAGCTGATC
CACGACGACA
GCCTGACCTT
TAAAGAGGAC
ATCCAGAAAG
CCCAGGTGTC
CGGCCAGGGC
GATAGCCTGC
ACGAGCACAT
TGCCAATCTG
GCCGGCAGCC
CCGCCATTAA
GAAGGGCATC
CTGCAGACAG
TGAAGGTGGT
GGACGAGCTC
GTGAAAGTGA
TGGGCCGGCA
CAAGCCCGAG
AACATCGTGA
TCGAAATGGC
CAGAGAGAAC
CAGACCACCC
AGAAGGGACA
GAAGAACAGC
CGCGAGAGAA
TGAAGCGGAT
CGAAGAGGGC
ATCAAAGAGC
TGGGCAGCCA
GATCCTGAAA
GAACACCCCG
TGGAAAACAC
CCAGCTGCAG
AACGAGAAGC
TGTACCTGTA
CTACCTGCAG
AATGGGCGGG
ATATGTACGT
GGACCAGGAA
CTGGACATCA
ACCGGCTGTC
CGACTACGAT
GTGGACGCTA
TCGTGCCTCA
GAGCTTTCTG
AAGGACGACT
CCATCGATAA
CAAAGTGCTG
ACTCGGAGCG
ACAAGAACCG
GGGCAAGAGC
GACAACGTGC
CCTCCGAAGA
GGTCGTGAAG
AAGATGAAGA
ACTACTGGCG
CCAGCTGCTG
AATGCCAAGC
TGATTACCCA
GAGGAAGTTC
GACAATCTGA
CCAAGGCCGA
GAGAGGCGGC
CTGAGCGAAC
TGGATAAGGC
CGGCTTCATC
AAGAGACAGC
TGGTGGAAAC
CCGGCAGATC
ACAAAGCACG
TGGCACAGAT
CCTGGACTCC
CGGATGAACA
CTAAGTACGA
CGAGAACGAC
AAACTGATCC
GGGAAGTGAA
AGTGATCACC
CTGAAGTCCA
AGCTGGTGTC
CGATTTCCGG
AAGGATTTCC
AGTTTTACAA
AGTGCGCGAG
ATCAACAACT
ACCACCACGC
CCACGACGCC
TACCTGAACG
CCGTCGTGGG
AACCGCCCTG
ATCAAAAAGT
ACCCTAAGCT
GGAAAGCGAG
TTCGTGTACG
GCGACTACAA
GGTGTACGAC
GTGCGGAAGA
TGATCGCCAA
GAGCGAGCAG
GAAATCGGCA
AGGCTACCGC
CAAGTACTTC
TTCTACAGCA
ACATCATGAA
CTTTTTCAAG
ACCGAGATTA
CCCTGGCCAA
CGGCGAGATC
CGGAAGCGGC
CTCTGATCGA
GACAAACGGC
GAAACAGGCG
AGATCGTGTG
GGATAAGGGC
CGGGACTTTG
CCACCGTGCG
GAAAGTGCTG
TCTATGCCCC
AAGTGAATAT
CGTGAAAAAG
ACCGAGGTGC
AGACAGGCGG
CTTCAGCAAA
GAGTCTATCC
TGCCCAAGAG
GAACAGCGAC
AAGCTGATCG
CCAGAAAGAA
GGACTGGGAC
CCTAAGAAGT
ACGGCGGCTT
CGACAGCCCC
ACCGTGGCCT
ATTCTGTGCT
GGTGGTGGCC
AAAGTGGAAA
AGGGCAAGTC
CAAGAAACTG
AAGAGTGTGA
AAGAGCTGCT
GGGGATCACC
ATCATGGAAA
GAAGCAGCTT
CGAGAAGAAT
CCCATCGACT
TTCTGGAAGC
CAAGGGCTAC
AAAGAAGTGA
AAAAGGACCT
GATCATCAAG
CTGCCTAAGT
ACTCCCTGTT
CGAGCTGGAA
AACGGCCGGA
AGAGAATGCT
GGCCTCTGCC
GGCGAACTGC
AGAAGGGAAA
CGAACTGGCC
CTGCCCTCCA
AATATGTGAA
CTTCCTGTAC
CTGGCCAGCC
ACTATGAGAA
GCTGAAGGGC
TCCCCCGAGG
ATAATGAGCA
GAAACAGCTG
TTTGTGGAAC
AGCACAAACA
CTACCTGGAC
GAGATCATCG
AGCAGATCAG
CGAGTTCTCC
AAGAGAGTGA
TCCTGGCCGA
CGCTAATCTG
GACAAGGTGC
TGAGCGCCTA
CAACAAGCAC
AGAGACAAGC
CTATCAGAGA
GCAGGCCGAG
AATATCATCC
ACCTGTTTAC
CCTGACCAAT
CTGGGAGCCC
CTGCCGCCTT
CAAGTACTTT
GACACCACCA
TCGACCGGAA
GAGGTACACC
AGCACCAAAG
AGGTGCTGGA
CGCCACCCTG
ATCCACCAGA
GCATCACCGG
CCTGTACGAG
ACACGGATCG
ACCTGTCTCA
GCTGGGAGGC
GACGCCTATC
CCTATGACGT
GCCCGATTAT
GCCAGCCTGG
GCAGCGGCTC
CCCCAAGAAA
AAACGCAAGG
TGGAAGATCC
TAAGAAAAAG
CGGAAAGTGG
ACGGCATTGG
TAGTGGGAGC
AACGGCAGCA
GCGGATCCTA
AACCGGTGCG
GCCGCAATCG
ATCGCCCACC
CCAACTTGTT
TATTGCAGCT
TATAATGGTT
ACAAATAAAG
CAATAGCATC
ACAAATTTCA
CAAATAAAGC
ATTTTTTTCA
CTGCATTCTA
GTTGTGGTTT
GTCCAAACTC
ATCAATGTAT
CTTATCATGT
CTGTATACCG
TCGACCTCTA
GCTAGAGCTT
GGCGTAATCA
TGGTCATAGC
TGTTTCCTGT
GTGAAATTGT
TATCCGCTCA
CAATTCCACA
CAACATACGA
GCCGGAAGCA
TAAAGTGTAA
AGCCTGGGGT
GCCTAATGAG
TGAGCTAACT
CACATTAATT
GCGTTGCGCT
CACTGCCCGC
TTTCCAGTCG
GGAAACCTGT
CGTGCCAGCT
GCATTAATGA
ATCGGCCAAC
GCGCGGGGAG
AGGCGGTTTG
CGTATTGGGC
GCTCTTCCGC
TTCCTCGCTC
ACTGACTCGC
TGCGCTCGGT
CGTTCGGCTG
CGGCGAGCGG
TATCAGCTCA
CTCAAAGGAC
GGCCGCATAA
CTTCGTATAA
TGTATGCTAT
ACGAAGTTAT
AAGCTTGGGC
TGCAGGTCGA
GGGATCTCCA
TAAGAGAAGA
GGGACAGCTA
TGACTGGGAG
TAGTCAGGAG
AGGAGGAAAA
ATCTGGCTAG
TAAAACATGT
AAGGAAAATT
TTAGGGATGT
TAAAGAAAAA
AATAACACAA
AACAAAATAT
AAAAAAAATC
TAACCTCAAG
TCAAGGCTTT
TCTATGGAAT
AAGGAATGGA
CAGCAGGGGG
CTGTTTCATA
TACTGATGAC
CTCTTTATAG
CCAACCTTTG
TTCATGGCAG
CCAGCATATG
GGCATATGTT
GCCAAACTCT
AAACCAAATA
CTCATTCTGA
TGTTTTAAAT
GATTTGCCCT
CCCATATGTC
CTTCCGAGTG
AGAGACACAA
AAAATTCCAA
CACACTATTG
CAATGAAAAT
AAATTTCCTT
TATTAGCCAG
AAGTCAGATG
CTCAAGGGGC
TTCATGATGT
CCCCATAATT
TTTGGCAGAG
GGAAAAAGAT
CTCAGTGGTA
TTTGTGAGCC
AGGGCATTGG
CCACACCAGC
CACCACCTTC
TGATAGGCAG
CCTGCACCTG
AGGAGTGAAT
TCACGCGTTT
AGCCTGGCAG
CATATCGAGA
TCGAAGTCAT
CGAGAGCGTC
AGCAGGGAGC
ATATCCAGAT
CAAAATCATC
CAGGGCATCG
GCGGGCAGCA
TGTCCAGGTC
GAAGTCGTCC
AGGGCGTCGG
TGGGTCCGCC
GCTCTCGCAC
TTCAGCTGCT
TCTCGAGGCC
GCAGATGATC
AGTTCCAGGC
CGAACAGGAA
GGCGGGCTCG
GCGCCCTGTC
TGTCGAACAG
CTCGATGGCC
TGCTTCAGCA
GGGGGGGCAT
GCTGTCGGTG
GTGGGTGTCT
CTCTTTCCTC
TTTGGCGACC
TGGTGTTCCT
GTTCTTCCAG
CACGCAGCCC
AGGGTGAAGT
GGCCCACGGC
GCTCAGGGCG
TACAGGGCGT
TTTCCAGGCT
GAAGCCCTGC
TGGCACAGGA
AGGCCAGCTG
GTTTTCCAGT
GTCTCGTACT
GCTTCTCGGT
GGGTCTGGTG
CCCAGGTGCA
CTTTGGCGCC
GTCCCGGTGG
CTCAGCAGGG
CGCATCTGTA
GCTCTTGGCG
TTGTTCCGCA
GGAAGTCCTG
CCAGCTCTCG
CCTTCCAGAG
GGCAGCTGTG
GGTGTGGTGC
CGGTCCAGCA
TCTCGATGGG
CAGGGCGTCC
AGCAGGGCCC
GCTTGTTCTT
CACGTGCCAG
TACAGGGTGG
GCTGTTCCAC
GCCCAGTTTC
TGGGCCAGCT
TCCGGGTGGT
CAGGCCCTCG
ATGCCCACGC
CGTTCAGCAG
TTCCAGGGCG
CTGTTGATCA
CTTTGCTCTT
GTCCAGCCGG
CTCATGTCGA
CATTCTTTGC
CAAAATGATG
AGACAGCACA
ATAACCAGCA
CGTTGCCCAG
GAGCTGTAGG
AAAAAGAAGA
AGGCATGAAC
ATGGTTAGCA
GAGGCTCTAG
AGCCGCCGGT
CACACGCCAG
AAGCCGAACC
CCGCCCTGCC
CCGTCCCCCC
CGAAGGCAGC
CGTCCCCCCG
CGGACAGCCC
CGAGGCTGGA
GAGGGAGAAG
GGGACGGCGG
CGCGGCGACG
CACGAAGGCC
CTCCCCGCCC
ATTTCCTTCC
TGCCGGCGCC
GCACCGCTTC
GCCCCGCGCC
CGCTAGAGGG
GGTGCGGCGG
CGCCTCCCAG
ATTTCGGCTC
CGCACAGATT
TGGGACAAAG
GAAGTCCCTG
CGCCCTCTCG
CACGATTACC
ATAAAAGGCA
ATGGCTGCGG
CTCGCCGCGC
CTCGACAGCC
GCCGGCGCTC
CGGGGGCCGC
CGCGCCCCTC
CCCCGAGCCC
TCCCCGGCCC
GAGGCGGCCC
CGCCCCGCCC
GGCACCCCCA
CCTGCCGCCA
CCCCCCGCCC
GGCACGGCGA
GCCCCGCGCC
ACGCCCCGTA
CGGAGCCCCG
CACCCGAAGC
CGGGCCGTGC
TCAGCAACTC
GGGGAGGGGG
GTGCAGGGGG
GGTTGCAGCC
CGACCGACGC
GCCCACACCC
CCTGCTCACC
CCCCCACGCA
CACACCCCGC
ACGCAGCCTT
TGTTCCCCTC
GCAGCCCCCC
CCGCACCGCG
GGGCACCGCC
CCCGGCCGCG
CTCCCCTCGC
GCACACTGCG
GAGCGCACAA
AGCCCCGCGC
CGCGCCCGCA
GCGCTCACAG
CCGCCGGGCA
GCGCGGAGCC
GCACGCGGCG
CTCCCCACGC
ACACACACAC
GCACGCACCC
CCCGAGCCGC
TCCCCCCGCA
CAAAGGGCCC
TCCCGGAGCC
CCTCAAGGCT
TTCACGCAGC
CACAGAAAAG
AAACAAGCCG
TCATTAAACC
AAGCGCTAAT
TACAGCCCGG
AGGAGAAGGG
CCGTCCCGCC
CGCTCACCTG
TGGGAGTAAC
GCGGTCAGTC
AGAGCCGGGG
CGGGCGGCGC
GAGGCGGCGG
CGGAGCGGGG
CACGGGGCGA
AGGCAGCGTC
GCAGCGACTC
CCCGCCCGCC
GCGCGCTTCG
CTTTTTATAG
GGCCGCCGCC
GCCGCCGCCT
CGCCATAAAA
GGAAACTTTC
GGAGCGCGCC
GCTCTGATTG
GCTGCCGCCG
CACCTCTCCG
CCTCGCCCCG
CCCCGCCCCT
CGCCCCGCCC
CGCCCCGCCT
GGCGCGCGCC
CCCCCCCCCC
CCCCGCCCCC
ATCGCTGCAC
AAAATAATTA
AAAAATAAAT
AAATACAAAA
TTGGGGGTGG
GGAGGGGGGG
GAGATGGGGA
GAGTGAAGCA
GAACGTGGGG
CTCACCTCGA
CCATGGTAAT
AGCGATGACT
AATACGTAGA
TGTACTGCCA
AGTAGGAAAG
TCCCATAAGG
TCATGTACTG
GGCATAATGC
CAGGCGGGCC
ATTTACCGTC
ATTGACGTCA
ATAGGGGGCG
TACTTGGCAT
ATGATACACT
TGATGTACTG
CCAAGTGGGC
AGTTTACCGT
AAATACTCCA
CCCATTGACG
TCAATGGAAA
GTCCCTATTG
GCGTTACTAT
GGGAACATAC
GTCATTATTG
ACGTCAATGG
GCGGGGGTCG
TTGGGCGGTC
AGCCAGGCGG
GCCATTTACC
GTAAGTTATG
TAACGCGGAA
CTCCATATAT
GGGCTATGAA
CTAATGACCC
CGTAATTGAT
TACTATTAAT
AACTAGTCAA
TAATCAATGT
CGAGACCCAT
AGAGCCCACC
GCATCCCCAG
CATGCCTGCT
ATTGTCTTCC
CAATCCTCCC
CCTTGCTGTC
CTGCCCCACC
CCACCCCCCA
GAATAGAATG
ACACCTACTC
AGACAATGCG
ATGCAATTTC
CTCATTTTAT
TAGGAAAGGA
CAGTGGGAGT
GGCACCTTCC
AGGGTCAAGG
AAGGCACGGG
GGAGGGGCAA
ACAACAGATG
GCTGGCAACT
AGAAGGCACA
GTCGAGGCTG
ATCAGCGGGT
TTAAACTCAG
AAGAACTCGT
CAAGAAGGCG
ATAGAAGGCG
ATGCGCTGCG
AATCGGGAGC
GGCGATACCG
TAAAGCACGA
GGAAGCGGTC
AGCCCATTCG
CCGCCAAGCT
CTTCAGCAAT
ATCACGGGTA
GCCAACGCTA
TGTCCTGATA
GCGGTCCGCC
ACACCCAGCC
GGCCACAGTC
GATGAATCCA
GAAAAGCGGC
CATTTTCCAC
CATGATATTC
GGCAAGCAGG
CATCGCCATG
GGTCACGACG
AGATCATCGC
CGTCGGGCAT
GCGCGCCTTG
AGCCTGGCGA
ACAGTTCGGC
TGGCGCGAGC
CCCTGATGCT
CTTCGTCCAG
ATCATCCTGA
TCGACAAGAC
CGGCTTCCAT
CCGAGTACGT
GCTCGCTCGA
TGCGATGTTT
CGCTTGGTGG
TCGAATGGGC
AGGTAGCCGG
ATCAAGCGTA
TGCAGCCGCC
GCATTGCATC
AGCCATGATG
GATACTTTCT
CGGCAGGAGC
AAGGTGAGAT
GACAGGAGAT
CCTGCCCCGG
CACTTCGCCC
AATAGCAGCC
AGTCCCTTCC
CGCTTCAGTG
ACAACGTCGA
GCACAGCTGC
GCAAGGAACG
CCCGTCGTGG
CCAGCCACGA
TAGCCGCGCT
GCCTCGTCCT
GCAGTTCATT
CAGGGCACCG
GACAGGTCGG
TCTTGACAAA
AAGAACCGGG
CGCCCCTGCG
CTGACAGCCG
GAACACGGCG
GCATCAGAGC
AGCCGATTGT
CTGTTGTGCC
CAGTCATAGC
CGAATAGCCT
CTCCACCCAA
GCGGCCGGAG
AACCTGCGTG
CAATCCATCT
TGTTCAATGG
CCGATCCCAT
GGTGGCGGTA
CCCTCGAGCC
TAGGGCCGGG
ATTCTCCTCC
ACGTCACCGC
ATGTTAGAAG
ACTTCCTCTG
CCCTCTCCGC
TGCCAGATCT
CTCGAGGCCC
TGTGGGAGGA
AGAGAAGAGG
TCAGAAGCTT
ATAACTTCGT
ATAATGTATG
CTATACGAAG
TTATTGCCCC
ACTGTGGGGT
GGAGGGGACA
GATAAAAGTA
CCCAGAACCA
GAGCCACATT
AACCGGCCCT
GGGAATATAA
GGTGGTCCCA
GCTCGGGGAC
ACAGGATCCC
TGGAGGCAGC
AAACATGCTG
TCCTGAAGTG
GACATAGGGG
CCCGGGTTGG
AGGAAGAAGA
CTAGCTGAGC
TCTCGGACCC
CTGGAAGATG
CCATGACAGG
GGGCTGGAAG
AGCTAGCACA
GACTAGAGAG
GTAAGGGGGG
TAGGGGAGCT
GCCCAAATGA
AAGGAGTGAG
AGGTGACCCG
AATCCACAGG
AGAACGGGGT
GTCCAGGCAA
AGAAAGCAAG
AGGATGGAGA
GGTGGCTAAA
GCCAGGGAGA
CGGGGTACTT
TGGGGTTGTC
CAGAAAAACG
GTGATGATGC
AGGCCTACAA
GAAGGGGAGG
CGGGACGCAA
GGGAGACATC
CGTCGGAGAA
GGCCATCCTA
AGAAACGAGA
GATGGCACAG
GCCCCAGAAG
GAGAAGGAAA
AGGGAACCCA
GCGAGTGAAG
ACGGCATGGG
GTTGGGTGAG
GGAGGAGAGA
TGCCCGGAGA
GGACCCAGAC
ACGGGGAGGA
TCCGCTCAGA
GGACATCACG
TGGTGCAGCG
CCGAGAAGGA
AGTGCTCCGG
AAAGAGCATC
CTTGGGCAGC
AACACAGCAG
AGAGCAAGGG
GAAGAGGGAG
TGGAGGAAGA
CGGAACCTGA
AGGAGGCGGC
AGGGAAGGAT
CTGGGCCAGC
CGTAGAGGTG
ACCCAGGCCA
CAAGCTGCAG
ACAGAAAGCG
GCACAGGCCC
AGGGGAGAGA
ATGCTGGTCA
GAGAAAGCA