名称: | (不稳定)pPtGE34 |
---|---|
别称: | Plasmid#107932 |
基因长: | |
质粒宿主: | 藻类 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | Zeo,His3 |
荧光标记: | |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | EPI300 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GGAAATTGTA
AACGTTAATA
TTTTGTTAAA
ATTCGCGTTA
AATTTTTGTT
AAATCAGCTC
ATTTTTTAAC
CAATAGGCCG
AAATCGGCAA
AATCCCTTAT
AAATCAAAAG
AATAGACCGA
GATAGGGTTG
AGTGTTGTTC
CAGTTTGGAA
CAAGAGTCCA
CTATTAAAGA
ACGTGGACTC
CAACGTCAAA
GGGCGAAAAA
CCGTCTATCA
GGGCGATGGC
CCACTACGTG
AACCATCACC
CTAATCAAGT
TTTTTGGGGT
CGAGGTGCCG
TAAAGCACTA
AATCGGAACC
CTAAAGGGAG
CCCCCGATTT
AGAGCTTGAC
GGGGAAAGCC
GGCGAACGTG
GCGAGAAAGG
AAGGGAAGAA
AGCGAAAGGA
GCGGGCGCTA
GGGCGCTGGC
AAGTGTAGCG
GTCACGCTGC
GCGTAACCAC
CACACCCGCC
GCGCTTAATG
CGCCGCTACA
GGGCGCGTCG
CGCCATTCGC
CATTCAGGCT
GCGCAACTGT
TGGGAAGGGC
GATCGGTGCG
GGCCTCTTCG
CTATTACGCC
AGCTGGCGAA
AGGGGGATGT
GCTGCAAGGC
GATTAAGTTG
GGTAACGCCA
GGGTTTTCCC
AGTCACGACG
TTGTAAAACG
ACGGCCAGTG
AATTGTAATA
CGACTCACTA
TAGGGCGAAT
TGGAGCTGAT
CGTCTTGCCT
TGCTCGTCGG
TGATGTACTT
CACCAGCTCC
GCGAAGTCGC
TCTTCTTGAT
GGAGCGCATG
GGGACGTGCT
TGGCAATCAC
GCGCACCCCC
CGGCCGTTTT
AGCGGCTAAA
AAAGTCATGG
CTCTGCCCTC
GGGCGGACCA
CGCCCATCAT
GACCTTGCCA
AGCTCGTCCT
GCTTCTCTTC
GATCTTCGCC
AGCAGGGCGA
GGATCGTGGC
ATCACCGAAC
CGCGCCGTGC
GCGGGTCGTC
GGTGAGCCAG
AGTTTCAGCA
GGCCGCCCAG
GCGGCCCAGG
TCGCCATTGA
TGCGGGCCAG
CTCGCGGACG
TGCTCATAGT
CCACGACGCC
CGTGATTTTG
TAGCCCTGGC
CGACGGCCAG
CAGGTAGGCC
GACAGGCTCA
TGCCGGCCGC
CGCCGCCTTT
TCCTCAATCG
CTCTTCGTTC
GTCTGGAAGG
CAGTACACCT
TGATAGGTGG
GCTGCCCTTC
CTGGTTGGCT
TGGTTTCATC
AGCCATCCGC
TTGCCCTCAT
CTGTTACGCC
GGCGGTAGCC
GGCCAGCCTC
GCAGAGCAGG
ATTCCCGTTG
AGCACCGCCA
GGTGCGAATA
AGGGACAGTG
AAGAAGGAAC
ACCCGCTCGC
GGGTGGGCCT
ACTTCACCTA
TCCTGCCCGG
CTGACGCCGT
TGGATACACC
AAGGAAAGTC
TACACGAACC
CTTTGGCAAA
ATCCTGTATA
TCGTGCGAAA
AAGGATGGAT
ATACCGAAAA
AATCGCTATA
ATGACCCCGA
AGCAGGGTTA
TGCAGCGGAA
GATGCCATTC
GCCATTCAGG
CTGCGCAACT
GTTGGGAAGG
GCGATCGGTG
CGGGCCTCTT
CGCTATTACG
CCAGCTGGCG
AAAGGGGGAT
GTGCTGCAAG
GCGATTAAGT
TGGGTAACGC
CAGGGTTTTC
CCAGTCACGA
CGTTGTAAAA
CGACGGCCAG
TGACCATGAT
TACGCCAAGC
TCGAAATTAA
CCCTCACTAA
AGGGAACAAA
AGCTGGTACC
TAACCCTGCG
ATAGACCTTT
TCCAAACTCA
CGCAGTCCAA
GAAAACAAAG
GGGTGAGAAG
TATACGCACC
TTTCGGTTTC
GGCATAATTC
TTAAACTCTT
GTGGTCACTT
TCTTGTGAAG
AAGCTAGGGG
CACTCGTTTT
CCCTCAGAGC
CTGCAAACAC
AAAATTCCTG
CAGTCAATTG
TCCCAACACT
CGGCAAGCCG
TATGCGCAAG
CAACGATGCG
CAGAAGGCCG
TGGATGGATG
GCGACTCGCG
ATATGGCTTC
TTGGGTCGCC
AGTGTGGTAT
GTCCGGCGTA
TGTCAATACG
CGAATTCGGA
CGACTGGCAT
CTCTAGGAGG
AGGATTCCTT
CTTTTATGAC
ATGTTTATTT
TATATACATT
GATGCTTTCC
GACAGTCGGA
AGTAATAAAT
GAATTTATTT
CAAGACTACC
TATACTCCTT
TGACTTGTTC
GACTAATCTT
ACCGCTTACT
AAAATCTCGA
AATCACGCTT
GACCTCTCGC
ACGCAAATTT
TTGCTGCTGG
ACGCTACGCA
CTCGGCCCAA
TTCTTCTCGG
TCCTCGTCGT
CGCAATTGTC
GTTGCGTTGA
TCTTGCACCG
AAGGAATCAG
AGAATAGAAT
ACCATGGCCA
AGTTGACCAG
TGCCGTTCCG
GTGCTCACCG
CGCGCGACGT
CGCCGGAGCG
GTCGAGTTCT
GGACCGACCG
GCTCGGGTTC
TCCCGGGACT
TCGTGGAGGA
CGACTTCGCC
GGTGTGGTCC
GGGACGACGT
GACCCTGTTC
ATCAGCGCGG
TCCAGGACCA
GGTGGTGCCG
GACAACACCC
TGGCCTGGGT
GTGGGTGCGC
GGCCTGGACG
AGCTGTACGC
CGAGTGGTCG
GAGGTCGTGT
CCACGAACTT
CCGGGACGCC
TCCGGGCCGG
CCATGACCGA
GATCGGCGAG
CAGCCGTGGG
GGCGGGAGTT
CGCCCTGCGC
GACCCGGCCG
GCAACTGCGT
GCACTTCGTG
GCCGAGGAGC
AGGACTGAAG
ATAAGAATAT
CTCATTGTGA
ACATCTATGA
TTTACCAATT
TTATTCTTTG
TTTACAGTTA
GACGCCAGTA
ATTGTGCTGT
TTCTCTCAAG
TCTGTGTCAA
TACAAACTAC
GAAACTTGGC
AATTTTTCTC
TTGAATATGA
GCACGAGATG
AAACGCACAA
AGGAAATTAG
TTTCCATCCT
TTGACAAAGT
TTGTTGCTGT
TTAGAGAACA
GATGTCAAAA
TTAACGTGCC
ATGGAATTGA
ACCATGGTGC
GCTATCCCCA
AATCACGTCG
TTTGACCTCG
TCACATTTAA
GGTATATCAA
GCATATTCAC
TTATATCTTG
AATCCTCCCT
GCTTGATATT
CCTTTGCCCT
TGAGCCATCT
TCCCCACACG
CTGGAGATGA
CCCCTGCCCC
TCCTTTCTTT
ATCCACCCGA
GTAAGGTGTC
GACAAGTCAC
TTTGCCCCTG
AGCCATCCTC
CCCACTCAGG
AGATGACCCC
TACCCCTTTC
TTCTTTATCC
ACCCCCACGT
GCTATAAAAA
TAATTATAAT
TTAAATTTTT
TAATATAAAT
ATATAAATTA
AAAATAGAAA
GTAAAAAAAG
AAATTAAAGA
AAAAATAGTT
TTTGTTTTCC
GAAGATGTAA
AAGACTCTAG
GGGGATCGCC
AACAAATACT
ACCTTTTACC
TTGCTCTTCC
TGCTCTCAGG
TATTAATGCC
GAATTGTTTC
ATCTTGTCTG
TGTAGAAGAC
CACACACGAA
AATCCTGTGA
TTTTACATTT
TACTTATCGT
TAATCGAATG
TATATCTATT
TAATCTGCTT
TTCTTGTCTA
ATAAATATAT
ATGTAAAGTA
CGCTTTTTGT
TGAAATTTTT
TAAACCTTTG
TTTATTTTTT
TTTCTTCATT
CCGTAACTCT
TCTACCTTCT
TTATTTACTT
TCTAAAATCC
AAATACAAAA
CATAAAAATA
AATAAACACA
GAGTAAATTC
CCAAATTATT
CCATCATTAA
AAGATACGAG
GCGCGTGTAA
GTTACAGGCA
AGCGATCCTA
GTACACTCTA
TATTTTTTTA
TGCCTCGGTA
ATGATTTTCA
TTTTTTTTTT
CCACCTAGCG
GATGACTCTT
TTTTTTTCTT
AGCGATTGGC
ATTATCACAT
AATGAATTAT
ACATTATATA
AAGTAATGTG
ATTTCTTCGA
AGAATATACT
AAAAAATGAG
CAGGCAAGAT
AAACGAAGGC
AAAGATGACA
GAGCAGAAAG
CCCTAGTAAA
GCGTATTACA
AATGAAACCA
AGATTCAGAT
TGCGATCTCT
TTAAAGGGTG
GTCCCCTAGC
GATAGAGCAC
TCGATCTTCC
CAGAAAAAGA
GGCAGAAGCA
GTAGCAGAAC
AGGCCACACA
ATCGCAAGTG
ATTAACGTCC
ACACAGGTAT
AGGGTTTCTG
GACCATATGA
TACATGCTCT
GGCCAAGCAT
TCCGGCTGGT
CGCTAATCGT
TGAGTGCATT
GGTGACTTAC
ACATAGACGA
CCATCACACC
ACTGAAGACT
GCGGGATTGC
TCTCGGTCAA
GCTTTTAAAG
AGGCCCTAGG
GGCCGTGCGT
GGAGTAAAAA
GGTTTGGATC
AGGATTTGCG
CCTTTGGATG
AGGCACTTTC
CAGAGCGGTG
GTAGATCTTT
CGAACAGGCC
GTACGCAGTT
GTCGAACTTG
GTTTGCAAAG
GGAGAAAGTA
GGAGATCTCT
CTTGCGAGAT
GATCCCGCAT
TTTCTTGAAA
GCTTTGCAGA
GGCTAGCAGA
ATTACCCTCC
ACGTTGATTG
TCTGCGAGGC
AAGAATGATC
ATCACCGTAG
TGAGAGTGCG
TTCAAGGCTC
TTGCGGTTGC
CATAAGAGAA
GCCACCTCGC
CCAATGGTAC
CAACGATGTT
CCCTCCACCA
AAGGTGTTCT
TTATGCAATC
TCGCCTATTC
ATGGTGTATA
AAAGTTCAAC
ATCCAAAGCT
AGAACTTTTG
GAAAGAGAAA
GAATATCCGA
ATAGGGCACG
GCGTGCCGTA
TTGTTGGAGT
GGACTAGCAG
AAAGTGAGGA
AGGCACAGGA
TGAGTTTTCT
CGAGACACAT
AGCTTCAGCG
TCGTGTAGGC
TAGGCAGAGG
TGAGTTTTCT
CGAGACATAC
CTTCAGCGTC
GTCTTCACTG
TCACAGTCAA
CTGACAGTAA
TCGTTGATCC
GGAGAGATTC
AAAATTCAAT
CTGTTTGGAC
CTGGATAAGA
CACAAGAGCG
ACATCCTGAC
ATGAACGCCG
TAAACAGCAA
ATCCTGGTTG
AACACGTATC
CTTTTGGGGG
CCTCCGCTAC
GACGCTCGCT
CCAGCTGGGG
CTTCCTTACT
ATACACAGCG
CGCATATTTC
ACGGTTGCCA
GAAGTCAAGA
TGGCCTCTAG
AATGGACTAT
AAGGACCACG
ACGGAGATTA
CAAGGATCAT
GATATTGATT
ACAAAGACGA
TGATGATAAA
ATGGCCCCAA
AGAAGAAGCG
GAAAGTCGGT
ATACACGGAG
TCCCAGCAGC
CGACAAGAAG
TACTCGATTG
GATTGGACAT
CGGCACCAAC
TCTGTTGGAT
GGGCCGTCAT
CACGGACGAG
TACAAGGTGC
CCAGCAAGAA
ATTCAAAGTA
CTGGGAAACA
CTGACCGCCA
TTCGATCAAG
AAGAATTTGA
TTGGAGCTTT
GCTGTTTGAC
AGCGGCGAAA
CAGCGGAAGC
CACGCGGCTT
AAACGAACCG
CGAGACGAAG
ATATACGCGA
CGGAAGAACC
GTATCTGCTA
TCTACAAGAG
ATTTTCTCGA
ATGAGATGGC
AAAAGTCGAT
GACTCTTTCT
TTCACCGATT
GGAAGAATCC
TTCCTCGTTG
AAGAGGATAA
GAAACATGAG
CGGCACCCGA
TATTTGGAAA
CATTGTGGAC
GAGGTGGCTT
ACCATGAGAA
ATACCCGACT
ATTTACCACC
TGCGAAAGAA
ATTGGTGGAC
TCGACGGACA
AAGCGGATCT
TCGCCTAATC
TATTTGGCGC
TAGCACATAT
GATTAAGTTT
CGCGGCCACT
TCTTGATCGA
AGGCGACTTG
AATCCGGACA
ACAGTGATGT
TGACAAACTG
TTTATTCAAC
TTGTACAAAC
TTACAACCAG
CTCTTTGAAG
AAAATCCCAT
TAACGCTTCG
GGAGTGGACG
CGAAAGCCAT
CCTTTCTGCC
CGACTCAGCA
AGAGCCGACG
CCTAGAAAAT
TTGATTGCGC
AACTTCCCGG
CGAGAAAAAG
AATGGACTTT
TTGGAAATCT
TATTGCTTTG
TCGCTGGGCC
TTACGCCGAA
CTTTAAGAGC
AACTTCGACC
TTGCGGAAGA
TGCCAAATTG
CAACTGAGTA
AAGACACATA
TGACGACGAC
CTCGACAATC
TTTTGGCACA
AATAGGAGAT
CAGTACGCCG
ACCTCTTTTT
GGCGGCTAAG
AACTTGTCCG
ACGCCATTCT
GTTGTCGGAC
ATCCTTCGAG
TTAATACGGA
GATTACTAAG
GCGCCCTTGA
GCGCCTCTAT
GATCAAAAGA
TACGACGAGC
ATCACCAAGA
CCTGACCCTC
CTTAAAGCTC
TCGTCCGGCA
ACAGCTGCCT
GAGAAGTACA
AAGAGATTTT
CTTTGATCAA
AGCAAAAACG
GATACGCGGG
CTACATTGAC
GGGGGAGCCT
CGCAAGAAGA
GTTCTACAAG
TTTATTAAAC
CGATCCTGGA
AAAGATGGAC
GGCACGGAGG
AATTACTCGT
GAAACTTAAC
CGAGAGGACC
TTTTGCGTAA
GCAACGGACT
TTCGACAACG
GATCTATTCC
GCACCAAATC
CATCTCGGAG
AATTGCACGC
AATTCTGCGC
CGGCAAGAAG
ATTTTTATCC
ATTTCTCAAG
GACAACCGGG
AAAAGATTGA
GAAAATCTTA
ACCTTCCGCA
TACCCTACTA
CGTCGGCCCT
TTGGCCAGGG
GAAATTCGAG
ATTTGCCTGG
ATGACGCGAA
AGAGTGAGGA
AACTATTACT
CCCTGGAATT
TCGAAGAAGT
GGTAGACAAA
GGAGCTTCCG
CGCAATCCTT
TATCGAGCGC
ATGACCAACT
TCGATAAGAA
CCTCCCGAAT
GAGAAAGTCC
TACCCAAGCA
TTCGTTGCTC
TACGAATACT
TTACGGTGTA
TAATGAGCTG
ACTAAAGTCA
AATACGTTAC
AGAGGGAATG
CGAAAACCGG
CTTTCCTCTC
GGGAGAGCAG
AAAAAGGCCA
TAGTCGATCT
TCTCTTTAAA
ACGAACCGTA
AAGTTACCGT
GAAACAACTA
AAAGAGGACT
ACTTCAAGAA
AATCGAATGC
TTTGACTCCG
TTGAAATTTC
CGGCGTTGAA
GATCGGTTCA
ATGCCTCCTT
GGGAACATAC
CACGATCTCC
TGAAAATTAT
CAAAGACAAG
GACTTTCTTG
ACAATGAGGA
AAACGAGGAC
ATTCTTGAAG
ATATTGTCTT
GACGCTAACA
CTTTTTGAGG
ATCGAGAGAT
GATTGAGGAA
CGCCTTAAAA
CCTATGCGCA
TCTGTTCGAC
GACAAAGTTA
TGAAGCAATT
GAAGCGCCGG
AGATATACGG
GATGGGGAAG
GTTGAGTCGG
AAACTGATAA
ATGGCATACG
CGATAAGCAG
TCCGGCAAAA
CAATTCTCGA
TTTCTTGAAG
TCCGACGGAT
TTGCGAATCG
AAACTTCATG
CAACTGATCC
ATGACGATTC
GTTGACCTTT
AAAGAGGACA
TTCAAAAAGC
CCAAGTATCC
GGACAAGGAG
ATTCTTTGCA
CGAGCATATT
GCGAATCTTG
CTGGAAGTCC
GGCTATTAAA
AAGGGCATTC
TCCAGACAGT
AAAGGTCGTG
GATGAACTCG
TTAAAGTTAT
GGGCCGACAC
AAACCCGAGA
ACATAGTCAT
CGAAATGGCG
AGAGAGAACC
AGACCACCCA
AAAGGGACAG
AAAAATTCAC
GCGAGCGAAT
GAAGCGGATT
GAAGAGGGGA
TCAAAGAGCT
TGGCAGCCAA
ATTCTGAAAG
AACACCCGGT
TGAAAACACC
CAATTGCAGA
ACGAGAAGCT
CTACCTGTAC
TATCTTCAAA
ATGGGCGTGA
TATGTACGTC
GATCAAGAAT
TGGACATCAA
CCGGCTATCC
GATTACGATG
TGGACCATAT
TGTTCCTCAG
TCGTTTCTCA
AAGACGACTC
CATAGACAAT
AAGGTTCTAA
CGAGAAGCGA
CAAGAACCGC
GGCAAATCAG
ACAACGTCCC
CTCCGAGGAA
GTAGTTAAGA
AAATGAAGAA
TTACTGGCGC
CAATTGCTGA
ACGCTAAGCT
CATTACCCAA
AGAAAGTTTG
ACAATTTGAC
GAAAGCCGAG
CGAGGCGGGC
TCTCGGAACT
GGATAAGGCC
GGCTTCATTA
AAAGACAATT
GGTGGAAACG
CGACAAATCA
CAAAGCACGT
CGCACAGATT
CTCGACTCCC
GCATGAACAC
TAAGTATGAC
GAGAATGACA
AACTAATCCG
GGAAGTCAAA
GTGATTACCT
TGAAGTCCAA
GCTGGTTTCC
GATTTTCGTA
AAGATTTTCA
GTTTTACAAA
GTTCGCGAGA
TCAACAACTA
CCATCACGCG
CACGACGCTT
ACTTGAACGC
TGTCGTGGGA
ACGGCCTTGA
TTAAAAAATA
CCCTAAGCTG
GAAAGCGAGT
TCGTTTACGG
CGATTACAAG
GTGTATGACG
TACGCAAAAT
GATCGCGAAG
TCAGAGCAGG
AAATTGGCAA
AGCTACTGCG
AAGTACTTCT
TTTATAGCAA
CATAATGAAT
TTTTTCAAAA
CGGAGATTAC
CCTCGCTAAC
GGCGAGATTC
GCAAGCGCCC
TCTTATCGAG
ACAAACGGCG
AAACGGGGGA
GATTGTCTGG
GATAAAGGCC
GCGATTTTGC
GACTGTGCGG
AAAGTCTTGT
CGATGCCGCA
AGTGAATATA
GTCAAAAAGA
CGGAGGTGCA
AACAGGCGGC
TTTTCGAAAG
AATCTATCCT
TCCCAAAAGG
AACTCTGATA
AGTTGATTGC
GCGAAAAAAG
GATTGGGACC
CTAAGAAGTA
TGGCGGCTTT
GACAGCCCGA
CCGTAGCATA
TTCTGTCCTT
GTGGTGGCCA
AAGTCGAAAA
GGGCAAATCC
AAGAAACTGA
AGAGTGTGAA
AGAATTGCTT
GGGATTACGA
TCATGGAAAG
ATCATCTTTT
GAGAAGAATC
CCATCGACTT
TCTCGAAGCT
AAAGGCTACA
AAGAAGTGAA
AAAGGACCTG
ATTATCAAGT
TGCCTAAGTA
CTCCTTGTTT
GAGCTGGAAA
ATGGCCGTAA
GCGAATGCTC
GCTTCTGCCG
GCGAACTCCA
GAAGGGAAAC
GAATTGGCGC
TGCCCTCCAA
ATATGTTAAC
TTTCTTTACC
TTGCCTCGCA
CTATGAGAAG
CTCAAGGGCT
CCCCCGAGGA
TAATGAGCAA
AAACAACTGT
TTGTAGAACA
GCATAAGCAC
TACTTGGACG
AGATCATTGA
GCAAATTAGC
GAGTTCTCCA
AGCGAGTCAT
CCTTGCCGAC
GCTAATCTGG
ACAAAGTCCT
GTCCGCCTAC
AATAAGCACC
GCGATAAGCC
AATCCGAGAG
CAAGCGGAGA
ATATTATCCA
CTTGTTTACG
CTCACCAATC
TTGGAGCCCC
TGCCGCCTTT
AAGTACTTTG
ACACCACCAT
CGACCGCAAG
AGGTACACCA
GCACCAAAGA
AGTCTTGGAC
GCCACCCTCA
TTCACCAATC
AATCACCGGC
TTGTACGAGA
CACGCATCGA
CCTGTCTCAA
CTCGGAGGCG
ACAAAAGGCC
GGCGGCCACG
AAAAAGGCCG
GCCAGGCAAA
AAAGAAAAAG
TAATCTGGAT
CCCTACCTCG
ACTTTGGCTG
GGACACTTTC
AGTGAGGACA
AGAAGCTCCA
GAAGCGTGCT
ATCGAACTCA
ACCAGGGACG
TGCGGCACAA
ATGGGCATCC
TTGCTCTCAT
GGTGCACGAA
CAGTTGGGAG
TCTCTATCCT
TCCTTAAAAA
TTTAATTTTC
ATTAGTTGCA
GTCACTCCGC
TTTGGTTTCA
CAGTCAGGAA
TAACACTAGC
TCGTCTTCAC
TGCAGGTTGG
CTCGGAAGTT
GGTGTTGACG
GTGAGCTGGA
AATTGGTTGT
CGGTCACTGC
TAGCGAGAAG
AAAACGGAGG
ACAGAAGGAA
GTGAAACTCG
GTTCGTTCTC
GACAGCCTCA
CTGTCAATAT
GCTCATTTTC
AATCCTTAGC
GCTTTTAATG
TCGAATTGAC
GGTAAATTGA
ATAGGATCTA
TAATATCTAC
AAGGTACTTT
GACACGCCAA
GTATTCATTG
TTAGTCAACA
ATATTTTAGA
GCTTTATAAG
GTCAAAAAAC
ACCTTCAAAG
TCGAGGAGAC
CGAGAGAGGG
TCTCAGTTTT
AGAGCTAGAA
ATAGCAAGTT
AAAATAAGGC
TAGTCCGTTA
TCAACTTGAA
AAAGTGGCAC
CGAGTCGGTG
CTTTTTTTTA
GAACCGCTCA
CCCATGCTAT
CGTATGTCAT
TTACATTGAC
AAACTGCTAT
GAATGCGTTT
GCGATAACTT
CAGCTCGCTG
AAGCCCTAGA
CATTTCGTTT
GACTAAATTG
TTTTCCGGTT
GCTTTACTAC
CGACCGGTCT
GCGGGTAGCA
TGTCTTGGCT
TTTGCGAGTT
CGAGAGAATT
TATGGACAAG
TGCGTTTACA
GTTTGGAACA
CGAGTGTGAG
TGAGCGGAAT
TGGCTATCTA
TAACATTCTT
ATTTCAGGAG
GCTGGTGATT
AGCAGAATTT
TCAAAGATCT
CGAATCTAAA
ATCGCCATGG
GCTCGACGAA
CTTTTCTGCA
AGCTTATCGA
TACCGTCGAC
CTCGAGGGGG
GGCCCGGTAC
CAGCTTTTGT
TCCCTTTAGT
GAGGGTTAAT
TCCGAGCTTG
GCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGAGCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTG