名称: | dCas9-KRAB-MeCP2 |
---|---|
别称: | Plasmid#110821 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTTAGGCGTT
TTGCGCTGCT
TCGCGATGTA
CGGGCCAGAT
ATACGCGTTG
ACATTGATTA
TTGACTAGTT
ATTAATAGTA
ATCAATTACG
GGGTCATTAG
TTCATAGCCC
ATATATGGAG
TTCCGCGTTA
CATAACTTAC
GGTAAATGGC
CCGCCTGGCT
GACCGCCCAA
CGACCCCCGC
CCATTGACGT
CAATAATGAC
GTATGTTCCC
ATAGTAACGC
CAATAGGGAC
TTTCCATTGA
CGTCAATGGG
TGGAGTATTT
ACGGTAAACT
GCCCACTTGG
CAGTACATCA
AGTGTATCAT
ATGCCAAGTA
CGCCCCCTAT
TGACGTCAAT
GACGGTAAAT
GGCCCGCCTG
GCATTATGCC
CAGTACATGA
CCTTATGGGA
CTTTCCTACT
TGGCAGTACA
TCTACGTATT
AGTCATCGCT
ATTACCATGG
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTCTA
TATAAGCAGA
GCTCGTTTAG
TGAACCGTCA
GATCGCCTGG
AGACGCCATC
CACGCTGTTT
TGACCTCCAT
AGAAGACACC
GGGACCGATC
CAGCCTCCGG
ACTCTAGAGG
ATCGAACCCT
TGCCACCATG
GACAAGAAGT
ACTCCATTGG
GCTCGCTATC
GGCACAAACA
GCGTCGGCTG
GGCCGTCATT
ACGGACGAGT
ACAAGGTGCC
GAGCAAAAAA
TTCAAAGTTC
TGGGCAATAC
CGATCGCCAC
AGCATAAAGA
AGAACCTCAT
TGGCGCCCTC
CTGTTCGACT
CCGGGGAGAC
GGCCGAAGCC
ACGCGGCTCA
AAAGAACAGC
ACGGCGCAGA
TATACCCGCA
GAAAGAATCG
GATCTGCTAC
CTGCAGGAGA
TCTTTAGTAA
TGAGATGGCT
AAGGTGGATG
ACTCTTTCTT
CCATAGGCTG
GAGGAGTCCT
TTTTGGTGGA
GGAGGATAAA
AAGCACGAGC
GCCACCCAAT
CTTTGGCAAT
ATCGTGGACG
AGGTGGCGTA
CCATGAAAAG
TACCCAACCA
TATATCATCT
GAGGAAGAAG
CTTGTAGACA
GTACTGATAA
GGCTGACTTG
CGGTTGATCT
ATCTCGCGCT
GGCGCATATG
ATCAAATTTC
GGGGACACTT
CCTCATCGAG
GGGGACCTGA
ACCCAGACAA
CAGCGATGTC
GACAAACTCT
TTATCCAACT
GGTTCAGACT
TACAATCAGC
TTTTCGAAGA
GAACCCGATC
AACGCATCCG
GAGTTGACGC
CAAAGCAATC
CTGAGCGCTA
GGCTGTCCAA
ATCCCGGCGG
CTCGAAAACC
TCATCGCACA
GCTCCCTGGG
GAGAAGAAGA
ACGGCCTGTT
TGGTAATCTT
ATCGCCCTGT
CACTCGGGCT
GACCCCCAAC
TTTAAATCTA
ACTTCGACCT
GGCCGAAGAT
GCCAAGCTTC
AACTGAGCAA
AGACACCTAC
GATGATGATC
TCGACAATCT
GCTGGCCCAG
ATCGGCGACC
AGTACGCAGA
CCTTTTTTTG
GCGGCAAAGA
ACCTGTCAGA
CGCCATTCTG
CTGAGTGATA
TTCTGCGAGT
GAACACGGAG
ATCACCAAAG
CTCCGCTGAG
CGCTAGTATG
ATCAAGCGCT
ATGATGAGCA
CCACCAAGAC
TTGACTTTGC
TGAAGGCCCT
TGTCAGACAG
CAACTGCCTG
AGAAGTACAA
GGAAATTTTC
TTCGATCAGT
CTAAAAATGG
CTACGCCGGA
TACATTGACG
GCGGAGCAAG
CCAGGAGGAA
TTTTACAAAT
TTATTAAGCC
CATCTTGGAA
AAAATGGACG
GCACCGAGGA
GCTGCTGGTA
AAGCTTAACA
GAGAAGATCT
GTTGCGCAAA
CAGCGCACTT
TCGACAATGG
AAGCATCCCC
CACCAGATTC
ACCTGGGCGA
ACTGCACGCT
ATCCTCAGGC
GGCAAGAGGA
TTTCTACCCC
TTTTTGAAAG
ATAACAGGGA
AAAGATTGAG
AAAATCCTCA
CATTTCGGAT
ACCCTACTAT
GTAGGCCCCC
TCGCCCGGGG
AAATTCCAGA
TTCGCGTGGA
TGACTCGCAA
ATCAGAAGAG
ACCATCACTC
CCTGGAACTT
CGAGGAAGTC
GTGGATAAGG
GGGCCTCTGC
CCAGTCCTTC
ATCGAAAGGA
TGACTAACTT
TGATAAAAAT
CTGCCTAACG
AAAAGGTGCT
TCCTAAACAC
TCTCTGCTGT
ACGAGTACTT
CACAGTTTAT
AACGAGCTCA
CCAAGGTCAA
ATACGTCACA
GAAGGGATGA
GAAAGCCAGC
ATTCCTGTCT
GGAGAGCAGA
AGAAAGCTAT
CGTGGACCTC
CTCTTCAAGA
CGAACCGGAA
AGTTACCGTG
AAACAGCTCA
AAGAAGACTA
TTTCAAAAAG
ATTGAATGTT
TCGACTCTGT
TGAAATCAGC
GGAGTGGAGG
ATCGCTTCAA
CGCATCCCTG
GGAACGTATC
ACGATCTCCT
GAAAATCATT
AAAGACAAGG
ACTTCCTGGA
CAATGAGGAG
AACGAGGACA
TTCTTGAGGA
CATTGTCCTC
ACCCTTACGT
TGTTTGAAGA
TAGGGAGATG
ATTGAAGAAC
GCTTGAAAAC
TTACGCTCAT
CTCTTCGACG
ACAAAGTCAT
GAAACAGCTC
AAGAGGCGCC
GATATACAGG
ATGGGGGCGG
CTGTCAAGAA
AACTGATCAA
TGGGATCCGA
GACAAGCAGA
GTGGAAAGAC
AATCCTGGAT
TTTCTTAAGT
CCGATGGATT
TGCCAACCGG
AACTTCATGC
AGTTGATCCA
TGATGACTCT
CTCACCTTTA
AGGAGGACAT
CCAGAAAGCA
CAAGTTTCTG
GCCAGGGGGA
CAGTCTTCAC
GAGCACATCG
CTAATCTTGC
AGGTAGCCCA
GCTATCAAAA
AGGGAATACT
GCAGACCGTT
AAGGTCGTGG
ATGAACTCGT
CAAAGTAATG
GGAAGGCATA
AGCCCGAGAA
TATCGTTATC
GAGATGGCCC
GAGAGAACCA
AACTACCCAG
AAGGGACAGA
AGAACAGTAG
GGAAAGGATG
AAGAGGATTG
AAGAGGGTAT
AAAAGAACTG
GGGTCCCAAA
TCCTTAAGGA
ACACCCAGTT
GAAAACACCC
AGCTTCAGAA
TGAGAAGCTC
TACCTGTACT
ACCTGCAGAA
CGGCAGGGAC
ATGTACGTGG
ATCAGGAACT
GGACATCAAT
CGGCTCTCCG
ACTACGACGT
GGCTGCTATC
GTGCCCCAGT
CTTTTCTCAA
AGATGATTCT
ATTGATAATA
AAGTGTTGAC
AAGATCCGAT
AAAGCTAGAG
GGAAGAGTGA
TAACGTCCCC
TCAGAAGAAG
TTGTCAAGAA
AATGAAAAAT
TATTGGCGGC
AGCTGCTGAA
CGCCAAACTG
ATCACACAAC
GGAAGTTCGA
TAATCTGACT
AAGGCTGAAC
GAGGTGGCCT
GTCTGAGTTG
GATAAAGCCG
GCTTCATCAA
AAGGCAGCTT
GTTGAGACAC
GCCAGATCAC
CAAGCACGTG
GCCCAAATTC
TCGATTCACG
CATGAACACC
AAGTACGATG
AAAATGACAA
ACTGATTCGA
GAGGTGAAAG
TTATTACTCT
GAAGTCTAAG
CTGGTCTCAG
ATTTCAGAAA
GGACTTTCAG
TTTTATAAGG
TGAGAGAGAT
CAACAATTAC
CACCATGCGC
ATGATGCCTA
CCTGAATGCA
GTGGTAGGCA
CTGCACTTAT
CAAAAAATAT
CCCAAGCTTG
AATCTGAATT
TGTTTACGGA
GACTATAAAG
TGTACGATGT
TAGGAAAATG
ATCGCAAAGT
CTGAGCAGGA
AATAGGCAAG
GCCACCGCTA
AGTACTTCTT
TTACAGCAAT
ATTATGAATT
TTTTCAAGAC
CGAGATTACA
CTGGCCAATG
GAGAGATTCG
GAAGCGACCA
CTTATCGAAA
CAAACGGAGA
AACAGGAGAA
ATCGTGTGGG
ACAAGGGTAG
GGATTTCGCG
ACAGTCCGGA
AGGTCCTGTC
CATGCCGCAG
GTGAACATCG
TTAAAAAGAC
CGAAGTACAG
ACCGGAGGCT
TCTCCAAGGA
AAGTATCCTC
CCGAAAAGGA
ACAGCGACAA
GCTGATCGCA
CGCAAAAAAG
ATTGGGACCC
CAAGAAATAC
GGCGGATTCG
ATTCTCCTAC
AGTCGCTTAC
AGTGTACTGG
TTGTGGCCAA
AGTGGAGAAA
GGGAAGTCTA
AAAAACTCAA
AAGCGTCAAG
GAACTGCTGG
GCATCACAAT
CATGGAGCGA
TCAAGCTTCG
AAAAAAACCC
CATCGACTTT
CTCGAGGCGA
AAGGATATAA
AGAGGTCAAA
AAAGACCTCA
TCATTAAGCT
TCCCAAGTAC
TCTCTCTTTG
AGCTTGAAAA
CGGCCGGAAA
CGAATGCTCG
CTAGTGCGGG
CGAGCTGCAG
AAAGGTAACG
AGCTGGCACT
GCCCTCTAAA
TACGTTAATT
TCTTGTATCT
GGCCAGCCAC
TATGAAAAGC
TCAAAGGGTC
TCCCGAAGAT
AATGAGCAGA
AGCAGCTGTT
CGTGGAACAA
CACAAACACT
ACCTTGATGA
GATCATCGAG
CAAATAAGCG
AATTCTCCAA
AAGAGTGATC
CTCGCCGACG
CTAACCTCGA
TAAGGTGCTT
TCTGCTTACA
ATAAGCACAG
GGATAAGCCC
ATCAGGGAGC
AGGCAGAAAA
CATTATCCAC
TTGTTTACTC
TGACCAACTT
GGGCGCGCCT
GCAGCCTTCA
AGTACTTCGA
CACCACCATA
GACAGAAAGC
GGTACACCTC
TACAAAGGAG
GTCCTGGACG
CCACACTGAT
TCATCAGTCA
ATTACGGGGC
TCTATGAAAC
AAGAATCGAC
CTCTCTCAGC
TCGGTGGAGA
CAGCAGGGCT
GACCCCAAGA
AGAAGAGGAA
GGTGAGTGGT
GGAGGAAGTG
GCGGGTCAGG
GTCGATGGAC
GCGAAATCAC
TTACGGCATG
GTCGAGAACA
CTGGTTACGT
TCAAGGACGT
GTTTGTGGAC
TTTACACGTG
AGGAGTGGAA
ATTGCTGGAT
ACTGCGCAAC
AAATTGTGTA
TCGAAATGTC
ATGCTTGAGA
ATTACAAGAA
CCTCGTCAGT
CTCGGATACC
AGTTGACGAA
ACCGGATGTG
ATCCTTAGGC
TCGAAAAGGG
GGAAGAACCT
TGGCTGGTAT
CGGGAGGTGG
TTCGGGTGGC
TCTGGATCAA
GCCCAAAGAA
GAAACGGAAG
GTGGAAGCCT
CAGTGCAGGT
GAAAAGGGTG
CTGGAAAAAT
CCCCCGGCAA
ACTCCTCGTG
AAGATGCCCT
TCCAGGCTTC
CCCTGGCGGA
AAAGGTGAAG
GGGGTGGCGC
AACCACATCT
GCCCAGGTCA
TGGTCATCAA
GCGACCTGGA
AGGAAAAGAA
AGGCCGAGGC
TGACCCTCAG
GCCATTCCAA
AGAAACGGGG
ACGCAAGCCA
GGGTCCGTGG
TCGCAGCTGC
AGCAGCTGAG
GCTAAGAAAA
AGGCAGTGAA
GGAAAGCTCC
ATCCGCAGTG
TGCAGGAGAC
TGTCCTGCCC
ATCAAGAAGA
GGAAGACTAG
GGAGACCGTG
TCCATCGAGG
TCAAAGAAGT
GGTCAAGCCC
CTGCTCGTGT
CCACCCTGGG
CGAAAAATCT
GGAAAGGGGC
TCAAAACATG
CAAGTCACCT
GGACGGAAAA
GCAAGGAGTC
TAGTCCAAAG
GGGCGCTCAA
GCTCCGCTTC
TAGTCCCCCT
AAAAAGGAAC
ACCATCACCA
TCACCATCAC
GCCGAGTCTC
CTAAGGCTCC
TATGCCACTG
CTCCCACCAC
CTCCACCACC
TGAGCCACAG
TCAAGCGAAG
ACCCCATCAG
CCCACCCGAG
CCTCAGGATC
TGTCCTCTAG
TATTTGCAAA
GAGGAAAAGA
TGCCCAGAGC
AGGCAGCCTG
GAGAGTGATG
GCTGTCCAAA
AGAACCCGCC
AAGACCCAGC
CTATGGTGGC
AGCCGCTGCA
ACTACCACCA
CAACCACAAC
TACCACAGTG
GCCGAAAAAT
ACAAGCATCG
CGGCGAGGGC
GAACGAAAGG
ACATTGTGTC
AAGCTCCATG
CCCAGACCTA
ACCGGGAGGA
ACCAGTCGAT
AGTAGGACAC
CCGTGACTGA
GAGAGTCTCA
TAGAAGAATT
CGATCCCTAC
CGGTTAGTAA
TGAGTTTAAA
CGGGGGAGGC
TAACTGAAAC
ACGGAAGGAG
ACAATACCGG
AAGGAACCCG
CGCTATGACG
GCAATAAAAA
GACAGAATAA
AACGCACGGG
TGTTGGGTCG
TTTGTTCATA
AACGCGGGGT
TCGGTCCCAG
GGCTGGCACT
CTGTCGATAC
CCCACCGAGA
CCCCATTGGG
GCCAATACGC
CCGCGTTTCT
TCCTTTTCCC
CACCCCACCC
CCCAAGTTCG
GGTGAAGGCC
CAGGGCTCGC
AGCCAACGTC
GGGGCGGCAG
GCCCTCCATA
GTCGGTCGTT
CGGCTGCGGC
GAGCGGTATC
AGCTCACTCA
AAGGCGGTAA
TACGGTTATC
CACAGAATCA
GGGGATAACG
CAGGAAAGAA
CATGTGAGCA
AAAGGCCAGC
AAAAGGCCAG
GAACCGTAAA
AAGGCCGCGT
TGCTGGCGTT
TTTCCATAGG
CTCCGCCCCC
CTGACGAGCA
TCACAAAAAT
CGACGCTCAA
GTCAGAGGTG
GCGAAACCCG
ACAGGACTAT
AAAGATACCA
GGCGTTTCCC
CCTGGAAGCT
CCCTCGTGCG
CTCTCCTGTT
CCGACCCTGC
CGCTTACCGG
ATACCTGTCC
GCCTTTCTCC
CTTCGGGAAG
CGTGGCGCTT
TCTCATAGCT
CACGCTGTAG
GTATCTCAGT
TCGGTGTAGG
TCGTTCGCTC
CAAGCTGGGC
TGTGTGCACG
AACCCCCCGT
TCAGCCCGAC
CGCTGCGCCT
TATCCGGTAA
CTATCGTCTT
GAGTCCAACC
CGGTAAGACA
CGACTTATCG
CCACTGGCAG
CAGCCACTGG
TAACAGGATT
AGCAGAGCGA
GGTATGTAGG
CGGTGCTACA
GAGTTCTTGA
AGTGGTGGCC
TAACTACGGC
TACACTAGAA
GAACAGTATT
TGGTATCTGC
GCTCTGCTGA
AGCCAGTTAC
CTTCGGAAAA
AGAGTTGGTA
GCTCTTGATC
CGGCAAACAA
ACCACCGCTG
GTAGCGGTGG
TTTTTTTGTT
TGCAAGCAGC
AGATTACGCG
CAGAAAAAAA
GGATCTCAAG
AAGATCCTTT
GATCTTTTCT
ACGGGGTCTG
ACGCTCAGTG
GAACGAAAAC
TCACGTTAAG
GGATTTTGGT
CATGAGATTA
TCAAAAAGGA
TCTTCACCTA
GATCCTTTTA
AATTAAAAAT
GAAGTTTTAA
ATCAATCTAA
AGTATATATG
AGTAAACTTG
GTCTGACAGT
TACCAATGCT
TAATCAGTGA
GGCACCTATC
TCAGCGATCT
GTCTATTTCG
TTCATCCATA
GTTGCCTGAC
TCCCCGTCGT
GTAGATAACT
ACGATACGGG
AGGGCTTACC
ATCTGGCCCC
AGTGCTGCAA
TGATACCGCG
AGACCCACGC
TCACCGGCTC
CAGATTTATC
AGCAATAAAC
CAGCCAGCCG
GAAGGGCCGA
GCGCAGAAGT
GGTCCTGCAA
CTTTATCCGC
CTCCATCCAG
TCTATTAATT
GTTGCCGGGA
AGCTAGAGTA
AGTAGTTCGC
CAGTTAATAG
TTTGCGCAAC
GTTGTTGCCA
TTGCTACAGG
CATCGTGGTG
TCACGCTCGT
CGTTTGGTAT
GGCTTCATTC
AGCTCCGGTT
CCCAACGATC
AAGGCGAGTT
ACATGATCCC
CCATGTTGTG
CAAAAAAGCG
GTTAGCTCCT
TCGGTCCTCC
GATCGTTGTC
AGAAGTAAGT
TGGCCGCAGT
GTTATCACTC
ATGGTTATGG
CAGCACTGCA
TAATTCTCTT
ACTGTCATGC
CATCCGTAAG
ATGCTTTTCT
GTGACTGGTG
AGTACTCAAC
CAAGTCATTC
TGAGAATAGT
GTATGCGGCG
ACCGAGTTGC
TCTTGCCCGG
CGTCAATACG
GGATAATACC
GCGCCACATA
GCAGAACTTT
AAAAGTGCTC
ATCATTGGAA
AACGTTCTTC
GGGGCGAAAA
CTCTCAAGGA
TCTTACCGCT
GTTGAGATCC
AGTTCGATGT
AACCCACTCG
TGCACCCAAC
TGATCTTCAG
CATCTTTTAC
TTTCACCAGC
GTTTCTGGGT
GAGCAAAAAC
AGGAAGGCAA
AATGCCGCAA
AAAAGGGAAT
AAGGGCGACA
CGGAAATGTT
GAATACTCAT
ACTCTTCCTT
TTTCAATATT
ATTGAAGCAT
TTATCAGGGT
TATTGTCTCA
TGAGCGGATA
CATATTTGAA
TGTATTTAGA
AAAATAAACA
AATAGGGGTT
CCGCGCACAT
TTCCCCGAAA
AGTGCCACCT
GACGTCGACG
GATCGGGAGA
TCTCCCGATC
CCCTATGGTG
CACTCTCAGT
ACAATCTGCT
CTGATGCCGC
ATAGTTAAGC
CAGTATCTGC
TCCCTGCTTG
TGTGTTGGAG
GTCGCTGAGT
AGTGCGCGAG
CAAAATTTAA
GCTACAACAA
GGCAAGGCTT
GACCGACAAT
TGCATGAAGA
ATCTGCTTAG
G