名称: | pLVX-EF1alpha-SARS-CoV-2-NSP12-2xStrep-IRES-Puro |
---|---|
别称: | Plasmid#141378 |
基因长: | 2904bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 新冠病毒 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGGAAGGGCT
AATTCACTCC
CAAAGAAGAC
AAGATATCCT
TGATCTGTGG
ATCTACCACA
CACAAGGCTA
CTTCCCTGAT
TAGCAGAACT
ACACACCAGG
GCCAGGGGTC
AGATATCCAC
TGACCTTTGG
ATGGTGCTAC
AAGCTAGTAC
CAGTTGAGCC
AGATAAGGTA
GAAGAGGCCA
ATAAAGGAGA
GAACACCAGC
TTGTTACACC
CTGTGAGCCT
GCATGGGATG
GATGACCCGG
AGAGAGAAGT
GTTAGAGTGG
AGGTTTGACA
GCCGCCTAGC
ATTTCATCAC
GTGGCCCGAG
AGCTGCATCC
GGAGTACTTC
AAGAACTGCT
GATATCGAGC
TTGCTACAAG
GGACTTTCCG
CTGGGGACTT
TCCAGGGAGG
CGTGGCCTGG
GCGGGACTGG
GGAGTGGCGA
GCCCTCAGAT
CCTGCATATA
AGCAGCTGCT
TTTTGCCTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACTTGAAAG
CGAAAGGGAA
ACCAGAGGAG
CTCTCTCGAC
GCAGGACTCG
GCTTGCTGAA
GCGCGCACGG
CAAGAGGCGA
GGGGCGGCGA
CTGGTGAGTA
CGCCAAAAAT
TTTGACTAGC
GGAGGCTAGA
AGGAGAGAGA
TGGGTGCGAG
AGCGTCAGTA
TTAAGCGGGG
GAGAATTAGA
TCGCGATGGG
AAAAAATTCG
GTTAAGGCCA
GGGGGAAAGA
AAAAATATAA
ATTAAAACAT
ATAGTATGGG
CAAGCAGGGA
GCTAGAACGA
TTCGCAGTTA
ATCCTGGCCT
GTTAGAAACA
TCAGAAGGCT
GTAGACAAAT
ACTGGGACAG
CTACAACCAT
CCCTTCAGAC
AGGATCAGAA
GAACTTAGAT
CATTATATAA
TACAGTAGCA
ACCCTCTATT
GTGTGCATCA
AAGGATAGAG
ATAAAAGACA
CCAAGGAAGC
TTTAGACAAG
ATAGAGGAAG
AGCAAAACAA
AAGTAAGACC
ACCGCACAGC
AAGCGGCCGG
CCGCTGATCT
TCAGACCTGG
AGGAGGAGAT
ATGAGGGACA
ATTGGAGAAG
TGAATTATAT
AAATATAAAG
TAGTAAAAAT
TGAACCATTA
GGAGTAGCAC
CCACCAAGGC
AAAGAGAAGA
GTGGTGCAGA
GAGAAAAAAG
AGCAGTGGGA
ATAGGAGCTT
TGTTCCTTGG
GTTCTTGGGA
GCAGCAGGAA
GCACTATGGG
CGCAGCGTCA
ATGACGCTGA
CGGTACAGGC
CAGACAATTA
TTGTCTGGTA
TAGTGCAGCA
GCAGAACAAT
TTGCTGAGGG
CTATTGAGGC
GCAACAGCAT
CTGTTGCAAC
TCACAGTCTG
GGGCATCAAG
CAGCTCCAGG
CAAGAATCCT
GGCTGTGGAA
AGATACCTAA
AGGATCAACA
GCTCCTGGGG
ATTTGGGGTT
GCTCTGGAAA
ACTCATTTGC
ACCACTGCTG
TGCCTTGGAA
TGCTAGTTGG
AGTAATAAAT
CTCTGGAACA
GATTTGGAAT
CACACGACCT
GGATGGAGTG
GGACAGAGAA
ATTAACAATT
ACACAAGCTT
AATACACTCC
TTAATTGAAG
AATCGCAAAA
CCAGCAAGAA
AAGAATGAAC
AAGAATTATT
GGAATTAGAT
AAATGGGCAA
GTTTGTGGAA
TTGGTTTAAC
ATAACAAATT
GGCTGTGGTA
TATAAAATTA
TTCATAATGA
TAGTAGGAGG
CTTGGTAGGT
TTAAGAATAG
TTTTTGCTGT
ACTTTCTATA
GTGAATAGAG
TTAGGCAGGG
ATATTCACCA
TTATCGTTTC
AGACCCACCT
CCCAACCCCG
AGGGGACCCG
ACAGGCCCGA
AGGAATAGAA
GAAGAAGGTG
GAGAGAGAGA
CAGAGACAGA
TCCATTCGAT
TAGTGAACGG
ATCTCGACGG
TATCGCCTTT
AAAAGAAAAG
GGGGGATTGG
GGGGTACAGT
GCAGGGGAAA
GAATAGTAGA
CATAATAGCA
ACAGACATAC
AAACTAAAGA
ATTACAAAAA
CAAATTACAA
AAATTCAAAA
TTTTCGGGTT
TATTACAGGG
ACAGCAGAGA
TCCAGTTTAT
CGATGAGTAA
TTCATACAAA
AGGACTCGCC
CCTGCCTTGG
GGAATCCCAG
GGACCGTCGT
TAAACTCCCA
CTAACGTAGA
ACCCAGAGAT
CGCTGCGTTC
CCGCCCCCTC
ACCCGCCCGC
TCTCGTCATC
ACTGAGGTGG
AGAAGAGCAT
GCGTGAGGCT
CCGGTGCCCG
TCAGTGGGCA
GAGCGCACAT
CGCCCACAGT
CCCCGAGAAG
TTGGGGGGAG
GGGTCGGCAA
TTGAACCGGT
GCCTAGAGAA
GGTGGCGCGG
GGTAAACTGG
GAAAGTGATG
TCGTGTACTG
GCTCCGCCTT
TTTCCCGAGG
GTGGGGGAGA
ACCGTATATA
AGTGCAGTAG
TCGCCGTGAA
CGTTCTTTTT
CGCAACGGGT
TTGCCGCCAG
AACACAGGTA
AGTGCCGTGT
GTGGTTCCCG
CGGGCCTGGC
CTCTTTACGG
GTTATGGCCC
TTGCGTGCCT
TGAATTACTT
CCACGCCCCT
GGCTGCAGTA
CGTGATTCTT
GATCCCGAGC
TTCGGGTTGG
AAGTGGGTGG
GAGAGTTCGA
GGCCTTGCGC
TTAAGGAGCC
CCTTCGCCTC
GTGCTTGAGT
TGAGGCCTGG
CTTGGGCGCT
GGGGCCGCCG
CGTGCGAATC
TGGTGGCACC
TTCGCGCCTG
TCTCGCTGCT
TTCGATAAGT
CTCTAGCCAT
TTAAAATTTT
TGATGACCTG
CTGCGACGCT
TTTTTTCTGG
CAAGATAGTC
TTGTAAATGC
GGGCCAAGAT
CTGCACACTG
GTATTTCGGT
TTTTGGGGCC
GCGGGCGGCG
ACGGGGCCCG
TGCGTCCCAG
CGCACATGTT
CGGCGAGGCG
GGGCCTGCGA
GCGCGGCCAC
CGAGAATCGG
ACGGGGGTAG
TCTCAAGCTG
GCCGGCCTGC
TCTGGTGCCT
GGCCTCGCGC
CGCCGTGTAT
CGCCCCGCCC
TGGGCGGCAA
GGCTGGCCCG
GTCGGCACCA
GTTGCGTGAG
CGGAAAGATG
GCCGCTTCCC
GGCCCTGCTG
CAGGGAGCTC
AAAATGGAGG
ACGCGGCGCT
CGGGAGAGCG
GGCGGGTGAG
TCACCCACAC
AAAGGAAAAG
GGCCTTTCCG
TCCTCAGCCG
TCGCTTCATG
TGACTCCACG
GAGTACCGGG
CGCCGTCCAG
GCACCTCGAT
TAGTTCTCGA
GCTTTTGGAG
TACGTCGTCT
TTAGGTTGGG
GGGAGGGGTT
TTATGCGATG
GAGTTTCCCC
ACACTGAGTG
GGTGGAGACT
GAAGTTAGGC
CAGCTTGGCA
CTTGATGTAA
TTCTCCTTGG
AATTTGCCCT
TTTTGAGTTT
GGATCTTGGT
TCATTCTCAA
GCCTCAGACA
GTGGTTCAAA
GTTTTTTTCT
TCCATTTCAG
GTGTCGTGAG
GATCTATTTC
CGGTGAATTC
GCCGCCACCA
TGTCAGCAGA
CGCACAAAGT
TTTCTGAACC
GGGTATGCGG
AGTCAGTGCG
GCGAGGCTTA
CGCCCTGTGG
TACGGGAACA
TCAACGGACG
TTGTTTACCG
CGCCTTCGAC
ATATACAATG
ATAAAGTCGC
CGGCTTCGCA
AAGTTCCTTA
AAACGAATTG
CTGTAGGTTC
CAAGAAAAGG
ACGAGGATGA
TAATTTGATA
GACTCCTACT
TCGTGGTCAA
ACGACACACG
TTTTCAAATT
ATCAGCACGA
GGAAACGATA
TATAATCTTC
TTAAAGACTG
CCCTGCAGTG
GCAAAACACG
ACTTCTTTAA
GTTCCGCATT
GACGGGGATA
TGGTCCCCCA
TATCTCCCGA
CAGAGATTGA
CGAAATATAC
AATGGCGGAT
CTGGTGTACG
CCCTGAGACA
CTTCGATGAG
GGCAATTGTG
ATACACTCAA
GGAGATTCTC
GTGACCTACA
ATTGTTGCGA
TGATGACTAT
TTCAATAAGA
AAGACTGGTA
CGACTTCGTC
GAAAACCCTG
ATATATTGAG
GGTGTACGCA
AACCTGGGTG
AACGAGTAAG
ACAGGCACTT
CTGAAAACCG
TCCAATTCTG
CGACGCTATG
AGAAACGCGG
GCATTGTTGG
TGTGCTTACG
CTGGATAACC
AAGACCTCAA
TGGTAATTGG
TACGATTTTG
GGGACTTCAT
CCAAACCACT
CCTGGCTCAG
GCGTTCCCGT
GGTAGACAGC
TACTATTCTT
TGTTGATGCC
AATTCTTACA
CTCACAAGGG
CCCTGACCGC
TGAGTCACAC
GTCGATACGG
ACTTGACCAA
GCCCTACATC
AAGTGGGATC
TTCTGAAGTA
CGACTTTACC
GAGGAACGAT
TGAAGCTTTT
TGATCGCTAC
TTTAAATACT
GGGATCAGAC
GTACCACCCA
AACTGCGTGA
ATTGCCTGGA
TGACCGATGT
ATACTCCATT
GCGCGAACTT
TAACGTTTTG
TTCTCTACAG
TATTTCCTCC
GACGAGCTTC
GGACCGCTTG
TGAGAAAGAT
ATTCGTCGAC
GGCGTCCCAT
TTGTTGTCAG
CACTGGTTAC
CACTTTCGCG
AGTTGGGGGT
GGTCCACAAT
CAGGATGTCA
ATTTGCATTC
CTCCAGGCTT
TCTTTTAAAG
AGTTGCTTGT
TTATGCCGCT
GACCCCGCGA
TGCATGCAGC
CTCTGGGAAT
CTTTTGCTGG
ACAAAAGGAC
GACGTGCTTT
AGCGTTGCTG
CCCTTACAAA
TAACGTAGCA
TTTCAAACCG
TAAAACCCGG
TAATTTTAAT
AAAGATTTTT
ATGATTTTGC
AGTCTCTAAG
GGTTTTTTCA
AGGAGGGGAG
TAGTGTAGAG
CTTAAACATT
TTTTTTTCGC
TCAGGATGGT
AACGCTGCGA
TCAGCGACTA
CGACTACTAT
CGATACAATC
TCCCGACGAT
GTGTGATATC
AGGCAGTTGT
TGTTCGTTGT
AGAAGTGGTT
GATAAGTATT
TTGACTGTTA
TGACGGCGGC
TGTATCAATG
CCAACCAGGT
CATTGTGAAT
AACCTCGATA
AATCTGCCGG
GTTCCCCTTT
AACAAGTGGG
GGAAGGCGCG
ACTGTACTAC
GACAGCATGT
CTTACGAAGA
TCAGGACGCA
CTCTTTGCAT
ACACAAAGAG
GAATGTGATA
CCGACTATAA
CTCAAATGAA
CCTTAAGTAT
GCAATCAGCG
CGAAAAATCG
GGCTAGGACT
GTTGCCGGAG
TTAGCATATG
TAGTACCATG
ACAAACCGAC
AGTTCCACCA
GAAGTTGCTG
AAATCAATTG
CTGCGACCCG
GGGTGCGACT
GTAGTAATCG
GGACATCAAA
GTTCTACGGC
GGGTGGCATA
ACATGCTCAA
GACGGTCTAC
TCAGACGTTG
AGAATCCGCA
TCTTATGGGT
TGGGATTATC
CAAAGTGTGA
CAGGGCGATG
CCCAACATGT
TGCGGATAAT
GGCCTCCTTG
GTACTTGCAC
GGAAACATAC
AACCTGCTGT
AGTTTGTCAC
ACCGATTTTA
CCGACTGGCA
AACGAATGTG
CTCAGGTTCT
CAGTGAAATG
GTAATGTGTG
GCGGCTCCCT
CTACGTTAAG
CCCGGTGGCA
CAAGCTCTGG
AGACGCAACT
ACTGCCTATG
CAAACAGTGT
ATTCAACATC
TGCCAGGCTG
TCACGGCCAA
TGTTAACGCA
TTGCTTTCCA
CTGACGGAAA
TAAGATTGCA
GACAAATACG
TGAGAAATTT
GCAACATAGA
CTCTACGAAT
GCCTCTATCG
CAATCGCGAT
GTTGATACGG
ACTTCGTTAA
TGAGTTCTAT
GCTTACTTGC
GAAAGCACTT
CTCCATGATG
ATTCTGTCAG
ACGACGCTGT
AGTATGCTTT
AATTCAACGT
ATGCCTCCCA
GGGTCTGGTC
GCGTCCATAA
AAAATTTTAA
AAGCGTCCTG
TATTACCAAA
ATAACGTTTT
CATGAGCGAA
GCGAAATGTT
GGACCGAGAC
TGATCTTACT
AAGGGCCCTC
ATGAGTTCTG
CTCACAACAC
ACTATGTTGG
TCAAGCAGGG
CGACGATTAC
GTGTATCTTC
CTTATCCCGA
TCCTAGTAGG
ATTCTTGGAG
CAGGTTGCTT
CGTGGATGAT
ATCGTCAAAA
CGGACGGCAC
CCTCATGATA
GAGAGGTTCG
TCTCTTTGGC
TATAGACGCG
TATCCGCTTA
CTAAACATCC
TAACCAAGAA
TACGCGGATG
TATTCCATCT
TTATCTGCAG
TATATCAGAA
AGTTGCATGA
TGAGCTGACC
GGTCACATGT
TGGACATGTA
TAGCGTGATG
CTTACCAATG
ACAATACTTC
ACGGTACTGG
GAACCTGAAT
TTTACGAGGC
GATGTATACG
CCTCACACCG
TCCTGCAGCT
CGAAGGCGGC
GGGGGATGGA
GCCATCCACA
ATTCGAGAAA
GGCGGTGGTT
CAGGAGGAGG
TAGCGGGGGT
GGATCATGGT
CACATCCGCA
GTTTGAAAAG
TAAGGATCCC
GCCCCTCTCC
CTCCCCCCCC
CCTAACGTTA
CTGGCCGAAG
CCGCTTGGAA
TAAGGCCGGT
GTGCGTTTGT
CTATATGTTA
TTTTCCACCA
TATTGCCGTC
TTTTGGCAAT
GTGAGGGCCC
GGAAACCTGG
CCCTGTCTTC
TTGACGAGCA
TTCCTAGGGG
TCTTTCCCCT
CTCGCCAAAG
GAATGCAAGG
TCTGTTGAAT
GTCGTGAAGG
AAGCAGTTCC
TCTGGAAGCT
TCTTGAAGAC
AAACAACGTC
TGTAGCGACC
CTTTGCAGGC
AGCGGAACCC
CCCACCTGGC
GACAGGTGCC
TCTGCGGCCA
AAAGCCACGT
GTATAAGATA
CACCTGCAAA
GGCGGCACAA
CCCCAGTGCC
ACGTTGTGAG
TTGGATAGTT
GTGGAAAGAG
TCAAATGGCT
CTCCTCAAGC
GTATTCAACA
AGGGGCTGAA
GGATGCCCAG
AAGGTACCCC
ATTGTATGGG
ATCTGATCTG
GGGCCTCGGT
GCACATGCTT
TACATGTGTT
TAGTCGAGGT
TAAAAAAACG
TCTAGGCCCC
CCGAACCACG
GGGACGTGGT
TTTCCTTTGA
AAAACACGAT
GATAAGCTTG
CCACAACCCA
CAAGGAGACG
ACCTTCCATG
ACCGAGTACA
AGCCCACGGT
GCGCCTCGCC
ACCCGCGACG
ACGTCCCCCG
GGCCGTACGC
ACCCTCGCCG
CCGCGTTCGC
CGACTACCCC
GCCACGCGCC
ACACCGTCGA
CCCGGACCGC
CACATCGAGC
GGGTCACCGA
GCTGCAAGAA
CTCTTCCTCA
CGCGCGTCGG
GCTCGACATC
GGCAAGGTGT
GGGTCGCGGA
CGACGGCGCC
GCGGTGGCGG
TCTGGACCAC
GCCGGAGAGC
GTCGAAGCGG
GGGCGGTGTT
CGCCGAGATC
GGCCCGCGCA
TGGCCGAGTT
GAGCGGTTCC
CGGCTGGCCG
CGCAGCAACA
GATGGAAGGC
CTCCTGGCGC
CGCACCGGCC
CAAGGAGCCC
GCGTGGTTCC
TGGCCACCGT
CGGCGTCTCG
CCCGACCACC
AGGGCAAGGG
TCTGGGCAGC
GCCGTCGTGC
TCCCCGGAGT
GGAGGCGGCC
GAGCGCGCCG
GGGTGCCCGC
CTTCCTGGAG
ACCTCCGCGC
CCCGCAACCT
CCCCTTCTAC
GAGCGGCTCG
GCTTCACCGT
CACCGCCGAC
GTCGAGGTGC
CCGAAGGACC
GCGCACCTGG
TGCATGACCC
GCAAGCCCGG
TGCCTAGACG
CGTCTGGAAC
AATCAACCTC
TGGATTACAA
AATTTGTGAA
AGATTGACTG
GTATTCTTAA
CTATGTTGCT
CCTTTTACGC
TATGTGGATA
CGCTGCTTTA
ATGCCTTTGT
ATCATGCTAT
TGCTTCCCGT
ATGGCTTTCA
TTTTCTCCTC
CTTGTATAAA
TCCTGGTTGC
TGTCTCTTTA
TGAGGAGTTG
TGGCCCGTTG
TCAGGCAACG
TGGCGTGGTG
TGCACTGTGT
TTGCTGACGC
AACCCCCACT
GGTTGGGGCA
TTGCCACCAC
CTGTCAGCTC
CTTTCCGGGA
CTTTCGCTTT
CCCCCTCCCT
ATTGCCACGG
CGGAACTCAT
CGCCGCCTGC
CTTGCCCGCT
GCTGGACAGG
GGCTCGGCTG
TTGGGCACTG
ACAATTCCGT
GGTGTTGTCG
GGGAAGCTGA
CGTCCTTTCC
ATGGCTGCTC
GCCTGTGTTG
CCACCTGGAT
TCTGCGCGGG
ACGTCCTTCT
GCTACGTCCC
TTCGGCCCTC
AATCCAGCGG
ACCTTCCTTC
CCGCGGCCTG
CTGCCGGCTC
TGCGGCCTCT
TCCGCGTCTT
CGCCTTCGCC
CTCAGACGAG
TCGGATCTCC
CTTTGGGCCG
CCTCCCCGCC
TGGAATTAAT
TCTGCAGTCG
AGACCTAGAA
AAACATGGAG
CAATCACAAG
TAGCAATACA
GCAGCTACCA
ATGCTGATTG
TGCCTGGCTA
GAAGCACAAG
AGGAGGAGGA
GGTGGGTTTT
CCAGTCACAC
CTCAGGTACC
TTTAAGACCA
ATGACTTACA
AGGCAGCTGT
AGATCTTAGC
CACTTTTTAA
AAGAAAAGAG
GGGACTGGAA
GGGCTAATTC
ACTCCCAACG
AAGACAAGAT
ATCCTTGATC
TGTGGATCTA
CCACACACAA
GGCTACTTCC
CTGATTAGCA
GAACTACACA
CCAGGGCCAG
GGGTCAGATA
TCCACTGACC
TTTGGATGGT
GCTACAAGCT
AGTACCAGTT
GAGCCAGATA
AGGTAGAAGA
GGCCAATAAA
GGAGAGAACA
CCAGCTTGTT
ACACCCTGTG
AGCCTGCATG
GGATGGATGA
CCCGGAGAGA
GAAGTGTTAG
AGTGGAGGTT
TGACAGCCGC
CTAGCATTTC
ATCACGTGGC
CCGAGAGCTG
CATCCGGAGT
ACTTCAAGAA
CTGCTGATAT
CGAGCTTGCT
ACAAGGGACT
TTCCGCTGGG
GACTTTCCAG
GGAGGCGTGG
CCTGGGCGGG
ACTGGGGAGT
GGCGAGCCCT
CAGATCCTGC
ATATAAGCAG
CTGCTTTTTG
CCTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
TAGTAGTTCA
TGTCATCTTA
TTATTCAGTA
TTTATAACTT
GCAAAGAAAT
GAATATCAGA
GAGTGAGAGG
CCTTGACATT
GCTAGCGTTT
ACCGTCGACC
TCTAGCTAGA
GCTTGGCGTA
ATCATGGTCA
TAGCTGTTTC
CTGTGTGAAA
TTGTTATCCG
CTCACAATTC
CACACAACAT
ACGAGCCGGA
AGCATAAAGT
GTAAAGCCTG
GGGTGCCTAA
TGAGTGAGCT
AACTCACATT
AATTGCGTTG
CGCTCACTGC
CCGCTTTCCA
GTCGGGAAAC
CTGTCGTGCC
AGCTGCATTA
ATGAATCGGC
CAACGCGCGG
GGAGAGGCGG
TTTGCGTATT
GGGCGCTCTT
CCGCTTCCTC
GCTCACTGAC
TCGCTGCGCT
CGGTCGTTCG
GCTGCGGCGA
GCGGTATCAG
CTCACTCAAA
GGCGGTAATA
CGGTTATCCA
CAGAATCAGG
GGATAACGCA
GGAAAGAACA
TGTGAGCAAA
AGGCCAGCAA
AAGGCCAGGA
ACCGTAAAAA
GGCCGCGTTG
CTGGCGTTTT
TCCATAGGCT
CCGCCCCCCT
GACGAGCATC
ACAAAAATCG
ACGCTCAAGT
CAGAGGTGGC
GAAACCCGAC
AGGACTATAA
AGATACCAGG
CGTTTCCCCC
TGGAAGCTCC
CTCGTGCGCT
CTCCTGTTCC
GACCCTGCCG
CTTACCGGAT
ACCTGTCCGC
CTTTCTCCCT
TCGGGAAGCG
TGGCGCTTTC
TCATAGCTCA
CGCTGTAGGT
ATCTCAGTTC
GGTGTAGGTC
GTTCGCTCCA
AGCTGGGCTG
TGTGCACGAA
CCCCCCGTTC
AGCCCGACCG
CTGCGCCTTA
TCCGGTAACT
ATCGTCTTGA
GTCCAACCCG
GTAAGACACG
ACTTATCGCC
ACTGGCAGCA
GCCACTGGTA
ACAGGATTAG
CAGAGCGAGG
TATGTAGGCG
GTGCTACAGA
GTTCTTGAAG
TGGTGGCCTA
ACTACGGCTA
CACTAGAAGA
ACAGTATTTG
GTATCTGCGC
TCTGCTGAAG
CCAGTTACCT
TCGGAAAAAG
AGTTGGTAGC
TCTTGATCCG
GCAAACAAAC
CACCGCTGGT
AGCGGTGGTT
TTTTTGTTTG
CAAGCAGCAG
ATTACGCGCA
GAAAAAAAGG
ATCTCAAGAA
GATCCTTTGA
TCTTTTCTAC
GGGGTCTGAC
GCTCAGTGGA
ACGAAAACTC
ACGTTAAGGG
ATTTTGGTCA
TGAGATTATC
AAAAAGGATC
TTCACCTAGA
TCCTTTTAAA
TTAAAAATGA
AGTTTTAAAT
CAATCTAAAG
TATATATGAG
TAAACTTGGT
CTGACAGTTA
CCAATGCTTA
ATCAGTGAGG
CACCTATCTC
AGCGATCTGT
CTATTTCGTT
CATCCATAGT
TGCCTGACTC
CCCGTCGTGT
AGATAACTAC
GATACGGGAG
GGCTTACCAT
CTGGCCCCAG
TGCTGCAATG
ATACCGCGAG
ACCCACGCTC
ACCGGCTCCA
GATTTATCAG
CAATAAACCA
GCCAGCCGGA
AGGGCCGAGC
GCAGAAGTGG
TCCTGCAACT
TTATCCGCCT
CCATCCAGTC
TATTAATTGT
TGCCGGGAAG
CTAGAGTAAG
TAGTTCGCCA
GTTAATAGTT
TGCGCAACGT
TGTTGCCATT
GCTACAGGCA
TCGTGGTGTC
ACGCTCGTCG
TTTGGTATGG
CTTCATTCAG
CTCCGGTTCC
CAACGATCAA
GGCGAGTTAC
ATGATCCCCC
ATGTTGTGCA
AAAAAGCGGT
TAGCTCCTTC
GGTCCTCCGA
TCGTTGTCAG
AAGTAAGTTG
GCCGCAGTGT
TATCACTCAT
GGTTATGGCA
GCACTGCATA
ATTCTCTTAC
TGTCATGCCA
TCCGTAAGAT
GCTTTTCTGT
GACTGGTGAG
TACTCAACCA
AGTCATTCTG
AGAATAGTGT
ATGCGGCGAC
CGAGTTGCTC
TTGCCCGGCG
TCAATACGGG
ATAATACCGC
GCCACATAGC
AGAACTTTAA
AAGTGCTCAT
CATTGGAAAA
CGTTCTTCGG
GGCGAAAACT
CTCAAGGATC
TTACCGCTGT
TGAGATCCAG
TTCGATGTAA
CCCACTCGTG
CACCCAACTG
ATCTTCAGCA
TCTTTTACTT
TCACCAGCGT
TTCTGGGTGA
GCAAAAACAG
GAAGGCAAAA
TGCCGCAAAA
AAGGGAATAA
GGGCGACACG
GAAATGTTGA
ATACTCATAC
TCTTCCTTTT
TCAATATTAT
TGAAGCATTT
ATCAGGGTTA
TTGTCTCATG
AGCGGATACA
TATTTGAATG
TATTTAGAAA
AATAAACAAA
TAGGGGTTCC
GCGCACATTT
CCCCGAAAAG
TGCCACCTGA
CGTCGACGGA
TCGGGAGATC
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GGATCAACTG
GATAACTCAA
GCTAACCAAA
ATCATCCCAA
ACTTCCCACC
CCATACCCTA
TTACCACTGC
CAATTACCTG
TGGTTTCATT
TACTCTAAAC
CTGTGATTCC
TCTGAATTAT
TTTCATTTTA
AAGAAATTGT
ATTTGTTAAA
TATGTACTAC
AAACTTAGTA
GT