名称: | pGWB21 |
---|---|
别称: | |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 蛋白表达 |
片段类型: | ORF,GateWay |
片段物种: | 空载体 |
原核抗性: | Kan |
筛选标记: | Hyg,Neo/G418 |
荧光标记: | |
启动子: | 35s |
复制子: | oriV |
拷贝数: | |
感受态: | DB3.1 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGAGCGTCGC
AAAGGCGCTC
GGTCTTGCCT
TGCTCGTCGG
TGATGTACTT
CACCAGCTCC
GCGAAGTCGC
TCTTCTTGAT
GGAGCGCATG
GGGACGTGCT
TGGCAATCAC
GCGCACCCCC
CGGCCGTTTT
AGCGGCTAAA
AAAGTCATGG
CTCTGCCCTC
GGGCGGACCA
CGCCCATCAT
GACCTTGCCA
AGCTCGTCCT
GCTTCTCTTC
GATCTTCGCC
AGCAGGGCGA
GGATCGTGGC
ATCACCGAAC
CGCGCCGTGC
GCGGGTCGTC
GGTGAGCCAG
AGTTTCAGCA
GGCCGCCCAG
GCGGCCCAGG
TCGCCATTGA
TGCGGGCCAG
CTCGCGGACG
TGCTCATAGT
CCACGACGCC
CGTGATTTTG
TAGCCCTGGC
CGACGGCCAG
CAGGTAGGCC
GACAGGCTCA
TGCCGGCCGC
CGCCGCCTTT
TCCTCAATCG
CTCTTCGTTC
GTCTGGAAGG
CAGTACACCT
TGATAGGTGG
GCTGCCCTTC
CTGGTTGGCT
TGGTTTCATC
AGCCATCCGC
TTGCCCTCAT
CTGTTACGCC
GGCGGTAGCC
GGCCAGCCTC
GCAGAGCAGG
ATTCCCGTTG
AGCACCGCCA
GGTGCGAATA
AGGGACAGTG
AAGAAGGAAC
ACCCGCTCGC
GGGTGGGCCT
ACTTCACCTA
TCCTGCCCGG
CTGACGCCGT
TGGATACACC
AAGGAAAGTC
TACACGAACC
CTTTGGCAAA
ATCCTGTATA
TCGTGCGAAA
AAGGATGGAT
ATACCGAAAA
AATCGCTATA
ATGACCCCGA
AGCAGGGTTA
TGCAGCGGAA
AAGCGCCACG
CTTCCCGAAG
GGAGAAAGGC
GGACAGGTAT
CCGGTAAGCG
GCAGGGTCGG
AACAGGAGAG
CGCACGAGGG
AGCTTCCAGG
GGGAAACGCC
TGGTATCTTT
ATAGTCCTGT
CGGGTTTCGC
CACCTCTGAC
TTGAGCGTCG
ATTTTTGTGA
TGCTCGTCAG
GGGGGCGGAG
CCTATGGAAA
AACGCCAGCA
ACGCGGCCTT
TTTACGGTTC
CTGGCCTTTT
GCTGGCCTTT
TGCTCACATG
TTCTTTCCTG
CGTTATCCCC
TGATTCTGTG
GATAACCGTA
TTACCGCCTT
TGAGTGAGCT
GATACCGCTC
GCCGCAGCCG
AACGACCGAG
CGCAGCGAGT
CAGTGAGCGA
GGAAGCGGAA
GAGCGCCAGA
AGGCCGCCAG
AGAGGCCGAG
CGCGGCCGTG
AGGCTTGGAC
GCTAGGGCAG
GGCATGAAAA
AGCCCGTAGC
GGGCTGCTAC
GGGCGTCTGA
CGCGGTGGAA
AGGGGGAGGG
GATGTTGTCT
ACATGGCTCT
GCTGTAGTGA
GTGGGTTGCG
CTCCGGCAGC
GGTCCTGATC
AATCGTCACC
CTTTCTCGGT
CCTTCAACGT
TCCTGACAAC
GAGCCTCCTT
TTCGCCAATC
CATCGACAAT
CACCGCGAGT
CCCTGCTCGA
ACGCTGCGTC
CGGACCGGCT
TCGTCGAAGG
CGTCTATCGC
GGCCCGCAAC
AGCGGCGAGA
GCGGAGCCTG
TTCAACGGTG
CCGCCGCGCT
CGCCGGCATC
GCTGTCGCCG
GCCTGCTCCT
CAAGCACGGC
CCCAACAGTG
AAGTAGCTGA
TTGTCATCAG
CGCATTGACG
GCGTCCCCGG
CCGAAAAACC
CGCCTCGCAG
AGGAAGCGAA
GCTGCGCGTC
GGCCGTTTCC
ATCTGCGGTG
CGCCCGGTCG
CGTGCCGGCA
TGGATGCGCG
CGCCATCGCG
GTAGGCGAGC
AGCGCCTGCC
TGAAGCTGCG
GGCATTCCCG
ATCAGAAATG
AGCGCCAGTC
GTCGTCGGCT
CTCGGCACCG
AATGCGTATG
ATTCTCCGCC
AGCATGGCTT
CGGCCAGTGC
GTCGAGCAGC
GCCCGCTTGT
TCCTGAAGTG
CCAGTAAAGC
GCCGGCTGCT
GAACCCCCAA
CCGTTCCGCC
AGTTTGCGTG
TCGTCAGACC
GTCTACGCCG
ACCTCGTTCA
ACAGGTCCAG
GGCGGCACGG
ATCACTGTAT
TCGGCTGCAA
CTTTGTCATG
CTTGACACTT
TATCACTGAT
AAACATAATA
TGTCCACCAA
CTTATCAGTG
ATAAAGAATC
CGCGCGTTCA
ATCGGACCAG
CGGAGGCTGG
TCCGGAGGCC
AGACGTGAAA
CCCAACATAC
CCCTGATCGT
AATTCTGAGC
ACTGTCGCGC
TCGACGCTGT
CGGCATCGGC
CTGATTATGC
CGGTGCTGCC
GGGCCTCCTG
CGCGATCTGG
TTCACTCGAA
CGACGTCACC
GCCCACTATG
GCATTCTGCT
GGCGCTGTAT
GCGTTGGTGC
AATTTGCCTG
CGCACCTGTG
CTGGGCGCGC
TGTCGGATCG
TTTCGGGCGG
CGGCCAATCT
TGCTCGTCTC
GCTGGCCGGC
GCCAGATCTG
GGGAACCCTG
TGGTTGGCAT
GCACATACAA
ATGGACGAAC
GGATAAACCT
TTTCACGCCC
TTTTAAATAT
CCGATTATTC
TAATAAACGC
TCTTTTCTCT
TAGGTTTACC
CGCCAATATA
TCCTGTCAAA
CACTGATAGT
TTAAACTGAA
GGCGGGAAAC
GACAATCTGA
TCATGAGCGG
AGAATTAAGG
GAGTCACGTT
ATGACCCCCG
CCGATGACGC
GGGACAAGCC
GTTTTACGTT
TGGAACTGAC
AGAACCGCAA
CGTTGAAGGA
GCCACTCAGC
CGCGGGTTTC
TGGAGTTTAA
TGAGCTAAGC
ACATACGTCA
GAAACCATTA
TTGCGCGTTC
AAAAGTCGCC
TAAGGTCACT
ATCAGCTAGC
AAATATTTCT
TGTCAAAAAT
GCTCCACTGA
CGTTCCATAA
ATTCCCCTCG
GTATCCAATT
AGAGTCTCAT
ATTCACTCTC
AATCCAAATA
ATCTGCACCG
GATCTGGATC
GTTTCGCATG
ATTGAACAAG
ATGGATTGCA
CGCAGGTTCT
CCGGCCGCTT
GGGTGGAGAG
GCTATTCGGC
TATGACTGGG
CACAACAGAC
AATCGGCTGC
TCTGATGCCG
CCGTGTTCCG
GCTGTCAGCG
CAGGGGCGCC
CGGTTCTTTT
TGTCAAGACC
GACCTGTCCG
GTGCCCTGAA
TGAACTGCAG
GACGAGGCAG
CGCGGCTATC
GTGGCTGGCC
ACGACGGGCG
TTCCTTGCGC
AGCTGTGCTC
GACGTTGTCA
CTGAAGCGGG
AAGGGACTGG
CTGCTATTGG
GCGAAGTGCC
GGGGCAGGAT
CTCCTGTCAT
CTCACCTTGC
TCCTGCCGAG
AAAGTATCCA
TCATGGCTGA
TGCAATGCGG
CGGCTGCATA
CGCTTGATCC
GGCTACCTGC
CCATTCGACC
ACCAAGCGAA
ACATCGCATC
GAGCGAGCAC
GTACTCGGAT
GGAAGCCGGT
CTTGTCGATC
AGGATGATCT
GGACGAAGAG
CATCAGGGGC
TCGCGCCAGC
CGAACTGTTC
GCCAGGCTCA
AGGCGCGCAT
GCCCGACGGC
GATGATCTCG
TCGTGACCCA
TGGCGATGCC
TGCTTGCCGA
ATATCATGGT
GGAAAATGGC
CGCTTTTCTG
GATTCATCGA
CTGTGGCCGG
CTGGGTGTGG
CGGACCGCTA
TCAGGACATA
GCGTTGGCTA
CCCGTGATAT
TGCTGAAGAG
CTTGGCGGCG
AATGGGCTGA
CCGCTTCCTC
GTGCTTTACG
GTATCGCCGC
TCCCGATTCG
CAGCGCATCG
CCTTCTATCG
CCTTCTTGAC
GAGTTCTTCT
GAGCGGGACT
CTGGGGTTCG
AAATGACCGA
CCAAGCGACG
CCCAACCTGC
CATCACGAGA
TTTCGATTCC
ACCGCCGCCT
TCTATGAAAG
GTTGGGCTTC
GGAATCGTTT
TCCGGGACGC
CGGCTGGATG
ATCCTCCAGC
GCGGGGATCT
CATGCTGGAG
TTCTTCGCCC
ACGGGATCTC
TGCGGAACAG
GCGGTCGAAG
GTGCCGATAT
CATTACGACA
GCAACGGCCG
ACAAGCACAA
CGCCACGATC
CTGAGCGACA
ATATGATCGG
GCCCGGCGTC
CACATCAACG
GCGTCGGCGG
CGACTGCCCA
GGCAAGACCG
AGATGCACCG
CGATATCTTG
CTGCGTTCGG
ATATTTTCGT
GGAGTTCCCG
CCACAGACCC
GGATGATCCC
CGATCGTTCA
AACATTTGGC
AATAAAGTTT
CTTAAGATTG
AATCCTGTTG
CCGGTCTTGC
GATGATTATC
ATATAATTTC
TGTTGAATTA
CGTTAAGCAT
GTAATAATTA
ACATGTAATG
CATGACGTTA
TTTATGAGAT
GGGTTTTTAT
GATTAGAGTC
CCGCAATTAT
ACATTTAATA
CGCGATAGAA
AACAAAATAT
AGCGCGCAAA
CTAGGATAAA
TTATCGCGCG
CGGTGTCATC
TATGTTACTA
GATCGGGCCT
CCTGTCAATG
CTGGCGGCGG
CTCTGGTGGT
GGTTCTGGTG
GCGGCTCTGA
GGGTGGTGGC
TCTGAGGGTG
GCGGTTCTGA
GGGTGGCGGC
TCTGAGGGAG
GCGGTTCCGG
TGGTGGCTCT
GGTTCCGGTG
ATTTTGATTA
TGAAAAGATG
GCAAACGCTA
ATAAGGGGGC
TATGACCGAA
AATGCCGATG
AAAACGCGCT
ACAGTCTGAC
GCTAAAGGCA
AACTTGATTC
TGTCGCTACT
GATTACGGTG
CTGCTATCGA
TGGTTTCATT
GGTGACGTTT
CCGGCCTTGC
TAATGGTAAT
GGTGCTACTG
GTGATTTTGC
TGGCTCTAAT
TCCCAAATGG
CTCAAGTCGG
TGACGGTGAT
AATTCACCTT
TAATGAATAA
TTTCCGTCAA
TATTTACCTT
CCCTCCCTCA
ATCGGTTGAA
TGTCGCCCTT
TTGTCTTTGG
CCCAATACGC
AAACCGCCTC
TCCCCGCGCG
TTGGCCGATT
CATTAATGCA
GCTGGCACGA
CAGGTTTCCC
GACTGGAAAG
CGGGCAGTGA
GCGCAACGCA
ATTAATGTGA
GTTAGCTCAC
TCATTAGGCA
CCCCAGGCTT
TACACTTTAT
GCTTCCGGCT
CGTATGTTGT
GTGGAATTGT
GAGCGGATAA
CAATTTCACA
CAGGAAACAG
CTATGACCAT
GATTACGCCA
AGCTTGCATG
CCTGCAGGTC
CCCAGATTAG
CCTTTTCAAT
TTCAGAAAGA
ATGCTAACCC
ACAGATGGTT
AGAGAGGCTT
ACGCAGCAGG
TCTCATCAAG
ACGATCTACC
CGAGCAATAA
TCTCCAGGAA
ATCAAATACC
TTCCCAAGAA
GGTTAAAGAT
GCAGTCAAAA
GATTCAGGAC
TAACTGCATC
AAGAACACAG
AGAAAGATAT
ATTTCTCAAG
ATCAGAAGTA
CTATTCCAGT
ATGGACGATT
CAAGGCTTGC
TTCACAAACC
AAGGCAAGTA
ATAGAGATTG
GAGTCTCTAA
AAAGGTAGTT
CCCACTGAAT
CAAAGGCCAT
GGAGTCAAAG
ATTCAAATAG
AGGACCTAAC
AGAACTCGCC
GTAAAGACTG
GCGAACAGTT
CATACAGAGT
CTCTTACGAC
TCAATGACAA
GAAGAAAATC
TTCGTCAACA
TGGTGGAGCA
CGACACACTT
GTCTACTCCA
AAAATATCAA
AGATACAGTC
TCAGAAGACC
AAAGGGCAAT
TGAGACTTTT
CAACAAAGGG
TAATATCCGG
AAACCTCCTC
GGATTCCATT
GCCCAGCTAT
CTGTCACTTT
ATTGTGAAGA
TAGTGGAAAA
GGAAGGTGGC
TCCTACAAAT
GCCATCATTG
CGATAAAGGA
AAGGCCATCG
TTGAAGATGC
CTCTGCCGAC
AGTGGTCCCA
AAGATGGACC
CCCACCCACG
AGGAGCATCG
TGGAAAAAGA
AGACGTTCCA
ACCACGTCTT
CAAAGCAAGT
GGATTGATGT
GATATCTCCA
CTGACGTAAG
GGATGACGCA
CAATCCCACT
ATCCTTCGCA
AGACCCTTCC
TCTATATAAG
GAAGTTCATT
TCATTTGGAG
AGAACACGGG
GGACTCTAGA
ATGAGCGGGT
TAATTAACGG
TGAACAAAAG
CTAATCTCCG
AGGAAGACTT
GAACGGTGAA
CAAAAATTAA
TCTCAGAAGA
AGACTTGAAC
GGACTCGACG
GTGAACAAAA
GTTGATTTCT
GAAGAAGATT
TGAACGGTGA
ACAAAAGCTA
ATCTCCGAGG
AAGACTTGAA
CGGTGAACAA
AAATTAATCT
CAGAAGAAGA
CTTGAACGGA
CTCGACGGTG
AACAAAAGTT
GATTTCTGAA
GAAGATTTGA
ACGGTGAACA
AAAGCTAATC
TCCGAGGAAG
ACTTGAACGG
TGAACAAAAA
TTAATCTCAG
AAGAAGACTT
GAACGGACTC
GACGGTGAAC
AAAAGTTGAT
TTCTGAAGAA
GATTTGAACG
GTGAACAAAA
GCTAATCTCC
GAGGAAGACT
TGAACGGTAG
CGCTGTTATC
ACAAGTTTGT
ACAAAAAAGC
TGAACGAGAA
ACGTAAAATG
ATATAAATAT
CAATATATTA
AATTAGATTT
TGCATAAAAA
ACAGACTACA
TAATACTGTA
AAACACAACA
TATCCAGTCA
CTATGGCGGC
CCCTCGAGGG
GCCGCATTAG
GCACCCCAGG
CTTTACACTT
TATGCTTCCG
GCTCGTATAA
TGTGTGGATT
TTGAGTTAGG
ATCCGGCGAG
ATTTTCAGGA
GCTAAGGAAG
CTAAAATGGA
GAAAAAAATC
ACTGGATATA
CCACCGTTGA
TATATCCCAA
TGGCATCGTA
AAGAACATTT
TGAGGCATTT
CAGTCAGTTG
CTCAATGTAC
CTATAACCAG
ACCGTTCAGC
TGGATATTAC
GGCCTTTTTA
AAGACCGTAA
AGAAAAATAA
GCACAAGTTT
TATCCGGCCT
TTATTCACAT
TCTTGCCCGC
CTGATGAATG
CTCATCCGGA
ATTCCGTATG
GCAATGAAAG
ACGGTGAGCT
GGTGATATGG
GATAGTGTTC
ACCCTTGTTA
CACCGTTTTC
CATGAGCAAA
CTGAAACGTT
TTCATCGCTC
TGGAGTGAAT
ACCACGACGA
TTTCCGGCAG
TTTCTACACA
TATATTCGCA
AGATGTGGCG
TGTTACGGTG
AAAACCTGGC
CTATTTCCCT
AAAGGGTTTA
TTGAGAATAT
GTTTTTCGTC
TCAGCCAATC
CCTGGGTGAG
TTTCACCAGT
TTTGATTTAA
ACGTGGCCAA
TATGGACAAC
TTCTTCGCCC
CCGTTTTCAC
CATGGGCAAA
TATTATACGC
AAGGCGACAA
GGTGCTGATG
CCGCTGGCGA
TTCAGGTTCA
TCATGCCGTC
TGTGATGGCT
TCCATGTCGG
CAGAATGCTT
AATGAATTAC
AACAGTACTG
CGATGAGTGG
CAGGGCGGGG
CGTAAACGCG
TGGATCCGGC
TTACTAAAAG
CCAGATAACA
GTATGCGTAT
TTGCGCGCTG
ATTTTTGCGG
TATAAGAATA
TATACTGATA
TGTATACCCG
AAGTATGTCA
AAAAGAGGTG
TGCTATGAAG
CAGCGTATTA
CAGTGACAGT
TGACAGCGAC
AGCTATCAGT
TGCTCAAGGC
ATATATGATG
TCAATATCTC
CGGTCTGGTA
AGCACAACCA
TGCAGAATGA
AGCCCGTCGT
CTGCGTGCCG
AACGCTGGAA
AGCGGAAAAT
CAGGAAGGGA
TGGCTGAGGT
CGCCCGGTTT
ATTGAAATGA
ACGGCTCTTT
TGCTGACGAG
AACAGGGACT
GGTGAAATGC
AGTTTAAGGT
TTACACCTAT
AAAAGAGAGA
GCCGTTATCG
TCTGTTTGTG
GATGTACAGA
GTGATATTAT
TGACACGCCC
GGGCGACGGA
TGGTGATCCC
CCTGGCCAGT
GCACGTCTGC
TGTCAGATAA
AGTCTCCCGT
GAACTTTACC
CGGTGGTGCA
TATCGGGGAT
GAAAGCTGGC
GCATGATGAC
CACCGATATG
GCCAGTGTGC
CGGTCTCCGT
TATCGGGGAA
GAAGTGGCTG
ATCTCAGCCA
CCGCGAAAAT
GACATCAAAA
ACGCCATTAA
CCTGATGTTC
TGGGGAATAT
AAATGTCAGG
CTCCCTTATA
CACAGCCAGT
CTGCAGGTCG
ACCATAGTGA
CTGGATATGT
TGTGTTTTAC
AGTATTATGT
AGTCTGTTTT
TTATGCAAAA
TCTAATTTAA
TATATTGATA
TTTATATCAT
TTTACGTTTC
TCGTTCAGCT
TTCTTGTACA
AAGTGGTGAT
AACCTAGAGC
TCGAATTTCC
CCGATCGTTC
AAACATTTGG
CAATAAAGTT
TCTTAAGATT
GAATCCTGTT
GCCGGTCTTG
CGATGATTAT
CATATAATTT
CTGTTGAATT
ACGTTAAGCA
TGTAATAATT
AACATGTAAT
GCATGACGTT
ATTTATGAGA
TGGGTTTTTA
TGATTAGAGT
CCCGCAATTA
TACATTTAAT
ACGCGATAGA
AAACAAAATA
TAGCGCGCAA
ACTAGGATAA
ATTATCGCGC
GCGGTGTCAT
CTATGTTACT
AGATCGGGAA
TTAGCTTCAT
CAACGCAAGA
CATGCGCACG
ACCGTCTGAC
AGGAGAGGAA
TTTCCGACGA
GCACAGAAAG
GACTTGCTCT
TGGACGTAGG
CCTATTTCTC
AGGCACATGT
ATCAAGTGTT
CGGACGTGGG
TTTTCGATGG
TGTATCAGCC
GCCGCCAACT
GGGAGATGAG
GAGGCTTTCT
TGGGGGGCAG
TCAGCAGTTC
ATTTCACAAG
ACAGAGGAAC
TTGTAAGGAG
ATGCACTGAT
TTATCTTGGC
GCAAACCAGC
AGGACGAATT
AGTGGGAATA
GCCCGCGAAT
ATCTAAGTTA
TGCCTGTCGG
CATGAGCAGA
AACTTCCAAT
TCGAAACAGT
TTGGAGAGGT
TGTTTTTGGG
CATACCTTTT
GTTAGTCAGC
CTCTCGATTG
CTCATCGTCA
TTACACAGTA
CCGAAGTTTG
ATCGATCTAG
TAACATAGAT
GACACCGCGC
GCGATAATTT
ATCCTAGTTT
GCGCGCTATA
TTTTGTTTTC
TATCGCGTAT
TAAATGTATA
ATTGCGGGAC
TCTAATCATA
AAAACCCATC
TCATAAATAA
CGTCATGCAT
TACATGTTAA
TTATTACATG
CTTAACGTAA
TTCAACAGAA
ATTATATGAT
AATCATCGCA
AGACCGGCAA
CAGGATTCAA
TCTTAAGAAA
CTTTATTGCC
AAATGTTTGA
ACGATCTGCT
TCGACGCACT
CCTTCTTTAC
TCCACCATCT
CGTCCTTATT
GAAAACGTGG
GTAGCACCAA
AACGAATCAA
GTCGCTGGAA
CTGAAGTTAC
CAATCACGCT
GGATGATTTG
CCAGTTGGAT
TAATCTTGCC
TTTCCCCGCA
TGAATAATAT
TGATGAATGC
ATGCGTGAGG
GGTATTTCGA
TTTTGGCAAT
AGCTGCAATT
GCCGCGACAT
CCTCCAACGA
GCATAATTCT
TCAGAAAAAT
AGCGATGTTC
CATGTTGTCA
GGGCATGCAT
GATGCACGTT
ATGAGGTGAC
GGTGCTAGGC
AGTATTCCCT
CAAAGTTTCA
TAGTCAGTAT
CATATTCATC
ATTGCATTCC
TGCAAGAGAG
AATTGAGACG
CAATCCACAC
GCTGCGGCAA
CCTTCCGGCG
TTCGTGGTCT
ATTTGCTCTT
GGACGTTGCA
AACGTAAGTG
TTGGATCCCG
GTCGGCATCT
ACTCTATTCC
TTTGCCCTCG
GACGAGTGCT
GGGGCGTCGG
TTTCCACTAT
CGGCGAGTAC
TTCTACACAG
CCATCGGTCC
AGACGGCCGC
GCTTCTGCGG
GCGATTTGTG
TACGCCCGAC
AGTCCCGGCT
CCGGATCGGA
CGATTGCGTC
GCATCGACCC
TGCGCCCAAG
CTGCATCATC
GAAATTGCCG
TCAACCAAGC
TCTGATAGAG
TTGGTCAAGA
CCAATGCGGA
GCATATACGC
CCGGAGCCGC
GGCGATCCTG
CAAGCTCCGG
ATGCCTCCGC
TCGAAGTAGC
GCGTCTGCTG
CTCCATACAA
GCCAACCACG
GCCTCCAGAA
GAAGATGTTG
GCGACCTCGT
ATTGGGAATC
CCCGAACATC
GCCTCGCTCC
AGTCAATGAC
CGCTGTTATG
CGGCCATTGT
CCGTCAGGAC
ATTGTTGGAG
CCGAAATCCG
CGTGCACGAG
GTGCCGGACT
TCGGGGCAGT
CCTCGGCCCA
AAGCATCAGC
TCATCGAGAG
CCTGCGCGAC
GGACGCACTG
ACGGTGTCGT
CCATCACAGT
TTGCCAGTGA
TACACATGGG
GATCAGCAAT
CGCGCATATG
AAATCACGCC
ATGTAGTGTA
TTGACCGATT
CCTTGCGGTC
CGAATGGGCC
GAACCCGCTC
GTCTGGCTAA
GATCGGCCGC
AGCGATCGCA
TCCATGGCCT
CCGCGACCGG
CTGCAGAACA
GCGGGCAGTT
CGGTTTCAGG
CAGGTCTTGC
AACGTGACAC
CCTGTGCACG
GCGGGAGATG
CAATAGGTCA
GGCTCTCGCT
GAATTCCCCA
ATGTCAAGCA
CTTCCGGAAT
CGGGAGCGCG
GCCGATGCAA
AGTGCCGATA
AACATAACGA
TCTTTGTAGA
AACCATCGGC
GCAGCTATTT
ACCCGCAGGA
CATATCCACG
CCCTCCTACA
TCGAAGCTGA
AAGCACGAGA
TTCTTCGCCC
TCCGAGAGCT
GCATCAGGTC
GGAGACGCTG
TCGAACTTTT
CGATCAGAAA
CTTCTCGACA
GACGTCGCGG
TGAGTTCAGG
CTTTTTCATA
TCGGGGTCGT
CCTCTCCAAA
TGAAATGAAC
TTCCTTATAT
AGAGGAAGGG
TCTTGCGAAG
GATAGTGGGA
TTGTGCGTCA
TCCCTTACGT
CAGTGGAGAT
ATCACATCAA
TCCACTTGCT
TTGAAGACGT
GGTTGGAACG
TCTTCTTTTT
CCACGATGCT
CCTCGTGGGT
GGGGGTCCAT
CTTTGGGACC
ACTGTCGGCA
GAGGCATCTT
GAACGATAGC
CTTTCCTTTA
TCGCAATGAT
GGCATTTGTA
GGTGCCACCT
TCCTTTTCTA
CTGTCCTTTT
GATGAAGTGA
CAGATAGCTG
GGCAATGGAA
TCCGAGGAGG
TTTCCCGATA
TTACCCTTTG
TTGAAAAGTC
TCAATAGCCC
TTTGGTCTTC
TGAGACTGTA
TCTTTGATAT
TCTTGGAGTA
GACGAGAGTG
TCGTGCTCCA
CCATGTTGAC
GGATCTCTAG
GACGCGTCCT
AGAAGCTAAT
TCACTGGCCG
TCGTTTTACA
ACGTCGTGAC
TGGGAAAACC
CTGGCGTTAC
CCAACTTAAT
CGCCTTGCAG
CACATCCCCC
TTTCGCCAGC
TGGCGTAATA
GCGAAGAGGC
CCGCACCGAT
CGCCCTTCCC
AACAGTTGCG
CAGCCTGAAT
GGCGCCCGCT
CCTTTCGCTT
TCTTCCCTTC
CTTTCTCGCC
ACGTTCGCCG
GCTTTCCCCG
TCAAGCTCTA
AATCGGGGGC
TCCCTTTAGG
GTTCCGATTT
AGTGCTTTAC
GGCACCTCGA
CCCCAAAAAA
CTTGATTTGG
GTGATGGTTC
ACGTAGTGGG
CCATCGCCCT
GATAGACGGT
TTTTCGCCCT
TTGACGTTGG
AGTCCACGTT
CTTTAATAGT
GGACTCTTGT
TCCAAACTGG
AACAACACTC
AACCCTATCT
CGGGCTATTC
TTTTGATTTA
TAAGGGATTT
TGCCGATTTC
GGAACCACCA
TCAAACAGGA
TTTTCGCCTG
CTGGGGCAAA
CCAGCGTGGA
CCGCTTGCTG
CAACTCTCTC
AGGGCCAGGC
GGTGAAGGGC
AATCAGCTGT
TGCCCGTCTC
ACTGGTGAAA
AGAAAAACCA
CCCCAGTACA
TTAAAAACGT
CCGCAATGTG
TTATTAAGTT
GTCTAAGCGT
CAATTTGTTT
ACACCACAAT
ATATCCTGCC
ACCAGCCAGC
CAACAGCTCC
CCGACCGGCA
GCTCGGCACA
AAATCACCAC
TCGATACAGG
CAGCCCATCA
GTCCGGGACG
GCGTCAGCGG
GAGAGCCGTT
GTAAGGCGGC
AGACTTTGCT
CATGTTACCG
ATGCTATTCG
GAAGAACGGC
AACTAAGCTG
CCGGGTTTGA
AACACGGATG
ATCTCGCGGA
GGGTAGCATG
TTGATTGTAA
CGATGACAGA
GCGTTGCTGC
CTGTGATCAA
ATATCATCTC
CCTCGCAGAG
ATCCGAATTA
TCAGCCTTCT
TATTCATTTC
TCGCTTAACC
GTGACAGGCT
GTCGATCTTG
AGAACTATGC
CGACATAATA
GGAAATCGCT
GGATAAAGCC
GCTGAGGAAG
CTGAGTGGCG
CTATTTCTTT
AGAAGTGAAC
GTTGACGATA
TCAACTCCCC
TATCCATTGC
TCACCGAATG
GTACAGGTCG
GGGACCCGAA
GTTCCGACTG
TCGGCCTGAT
GCATCCCCGG
CTGATCGACC
CCAGATCTGG
GGCTGAGAAA
GCCCAGTAAG
GAAACAACTG
TAGGTTCGAG
TCGCGAGATC
CCCCGGAACC
AAAGGAAGTA
GGTTAAACCC
GCTCCGATCA
GGCCGAGCCA
CGCCAGGCCG
AGAACATTGG
TTCCTGTAGG
CATCGGGATT
GGCGGATCAA
ACACTAAAGC
TACTGGAACG
AGCAGAAGTC
CTCCGGCCGC
CAGTTGCCAG
GCGGTAAAGG
TGAGCAGAGG
CACGGGAGGT
TGCCACTTGC
GGGTCAGCAC
GGTTCCGAAC
GCCATGGAAA
CCGCCCCCGC
CAGGCCCGCT
GCGACGCCGA
CAGGATCTAG
CGCTGCGTTT
GGTGTCAACA
CCAACAGCGC
CACGCCCGCA
GTTCCGCAAA
TAGCCCCCAG
GACCGCCATC
AATCGTATCG
GGCTACCTAG
CAGAGCGGCA
GAGATGAACA
CGACCATCAG
CGGCTGCACA
GCGCCTACCG
TCGCCGCGAC
CCCGCCCGGC
AGGCGGTAGA
CCGAAATAAA
CAACAAGCTC
CAGAATAGCG
AAATATTAAG
TGCGCCGAGG
ATGAAGATGC
GCATCCACCA
GATTCCCGTT
GGAATCTGTC
GGACGATCAT
CACGAGCAAT
AAACCCGCCG
GCAACGCCCG
CAGCAGCATA
CCGGCGACCC
CTCGGCCTCG
CTGTTCGGGC
TCCACGAAAA
CGCCGGACAG
ATGCGCCTTG
TGAGCGTCCT
TGGGGCCGTC
CTCCTGTTTG
AAGACCGACA
GCCCAATGAT
CTCGCCGTCG
ATGTAGGCGC
CGAATGCCAC
GGCATCTCGC
AACCGTTCAG
CGAACGCCTC
CATGGGCTTT
TTCTCCTCGT
GCTCGTAAAC
GGACCCGAAC
ATCTCTGGAG
CTTTCTTCAG
GGCCGACAAT
CGGATCTCGC
GGAAATCCTG
CACGTCGGCC
GCTCCAAGCC
GTCGAATCTG
AGCCTTAATC
ACAATTGTCA
ATTTTAATCC
TCTGTTTATC
GGCAGTTCGT
AGAGCGCGCC
GTGCGTCCCG
AGCGATACTG
AGCGAAGCAA
GTGCGTCGAG
CAGTGCCCGC
TTGTTCCTGA
AATGCCAGTA
AAGCGCTGGC
TGCTGAACCC
CCAGCCGGAA
CTGACCCCAC
AAGGCCCTAG
CGTTTGCAAT
GCACCAGGTC
ATCATTGACC
CAGGCGTGTT
CCACCAGGCC
GCTGCCTCGC
AACTCTTCGC
AGGCTTCGCC
GACCTGCTCG
CGCCACTTCT
TCACGCGGGT
GGAATCCGAT
CCGCACATGA
GGCGGAAGGT
TTCCAGCTTG
AGCGGGTACG
GCTCCCGGTG
CGAGCTGAAA
TAGTCGAACA
TCCGTCGGGC
CGTCGGCGAC
AGCTTGCGGT
ACTTCTCCCA
TATGAATTTC
GTGTAGTGGT
CGCCAGCAAA
CAGCACGACG
ATTTCCTCGT
CGATCAGGAC
CTGGCAACGG
GACGTTTTCT
TGCCACGGTC
CAGGACGCGG
AAGCGGTGCA
GCAGCGACAC
CGATTCCAGG
TGCCCAACGC
GGTCGGACGT
GAAGCCCATC
GCCGTCGCCT
GTAGGCGCGA
CAGGCATTCC
TCGGCCTTCG
TGTAATACCG
GCCATTGATC
GACCAGCCCA
GGTCCTGGCA
AAGCTCGTAG
AACGTGAAGG
TGATCGGCTC
GCCGATAGGG
GTGCGCTTCG
CGTACTCCAA
CACCTGCTGC
CACACCAGTT
CGTCATCGTC
GGCCCGCAGC
TCGACGCCGG
TGTAGGTGAT
CTTCACGTCC
TTGTTGACGT
GGAAAATGAC
CTTGTTTTGC
AGCGCCTCGC
GCGGGATTTT
CTTGTTGCGC
GTGGTGAACA
GGGCAGAGCG
GGCCGTGTCG
TTTGGCATCG
CTCGCATCGT
GTCCGGCCAC
GGCGCAATAT
CGAACAAGGA
AAGCTGCATT
TCCTTGATCT
GCTGCTTCGT
GTGTTTCAGC
AACGCGGCCT
GCTTGGCCTC
GCTGACCTGT
TTTGCCAGGT
CCTCGCCGGC
GGTTTTTCGC
TTCTTGGTCG
TCATAGTTCC
TCGCGTGTCG
ATGGTCATCG
ACTTCGCCAA
ACCTGCCGCC
TCCTGTTCGA
GACGACGCGA
ACGCTCCACG
GCGGCCGATG
GCGCGGGCAG
GGCAGGGGGA
GCCAGTTGCA
CGCTGTCGCG
CTCGATCTTG
GCCGTAGCTT
GCTGGACCAT
CGAGCCGACG
GACTGGAAGG
TTTCGCGGGG
CGCACGCATG
ACGGTGCGGC
TTGCGATGGT
TTCGGCATCC
TCGGCGGAAA
ACCCCGCGTC
GATCAGTTCT
TGCCTGTATG
CCTTCCGGTC
AAACGTCCGA
TTCATTCACC
CTCCTTGCGG
GATTGCCCCG
ACTCACGCCG
GGGCAATGTG
CCCTTATTCC
TGATTTGACC
CGCCTGGTGC
CTTGGTGTCC
AGATAATCCA
CCTTATCGGC
AATGAAGTCG
GTCCCGTAGA
CCGTCTGGCC
GTCCTTCTCG
TACTTGGTAT
TCCGAATCTT
GCCCTGCACG
AATACCAGCG
ACCCCTTGCC
CAAATACTTG
CCGTGGGCCT
CGGCCTGAGA
GCCAAAACAC
TTGATGCGGA
AGAAGTCGGT
GCGCTCCTGC
TTGTCGCCGG
CATCGTTGCG
CCACATCTAG
GTACTAAAAC
AATTCATCCA
GTAAAATATA
ATATTTTATT
TTCTCCCAAT
CAGGCTTGAT
CCCCAGTAAG
TCAAAAAATA
GCTCGACATA
CTGTTCTTCC
CCGATATCCT
CCCTGATCGA
CCGGACGCAG
AAGGCAATGT
CATACCACTT
GTCCGCCCTG
CCGCTTCTCC
CAAGATCAAT
AAAGCCACTT
ACTTTGCCAT
CTTTCACAAA
GATGTTGCTG
TCTCCCAGGT
CGCCGTGGGA
AAAGACAAGT
TCCTCTTCGG
GCTTTTCCGT
CTTTAAAAAA
TCATACAGCT
CGCGCGGATC
TTTAAATGGA
GTGTCTTCTT
CCCAGTTTTC
GCAATCCACA
TCGGCCAGAT
CGTTATTCAG
TAAGTAATCC
AATTCGGCTA
AGCGGCTGTC
TAAGCTATTC
GTATAGGGAC
AATCCGATAT
GTCGATGGAG
TGAAAGAGCC
TGATGCACTC
CGCATACAGC
TCGATAATCT
TTTCAGGGCT
TTGTTCATCT
TCATACTCTT
CCGAGCAAAG
GACGCCATCG
GCCTCACTCA
TGAGCAGATT
GCTCCAGCCA
TCATGCCGTT
CAAAGTGCAG
GACCTTTGGA
ACAGGCAGCT
TTCCTTCCAG
CCATAGCATC
ATGTCCTTTT
CCCGTTCCAC
ATCATAGGTG
GTCCCTTTAT
ACCGGCTGTC
CGTCATTTTT
AAATATAGGT
TTTCATTTTC
TCCCACCAGC
TTATATACCT
TAGCAGGAGA
CATTCCTTCC
GTATCTTTTA
CGCAGCGGTA
TTTTTCGATC
AGTTTTTTCA
ATTCCGGTGA
TATTCTCATT
TTAGCCATTT
ATTATTTCCT
TCCTCTTTTC
TACAGTATTT
AAAGATACCC
CAAGAAGCTA
ATTATAACAA
GACGAACTCC
AATTCACTGT
TCCTTGCATT
CTAAAACCTT
AAATACCAGA
AAACAGCTTT
TTCAAAGTTG
TTTTCAAAGT
TGGCGTATAA
CATAGTATCG
ACGGAGCCGA
TTTTGAAACC
ACAATTATGG
GTGATGCTGC
CAACTTACTG
ATTTAGTGTA
TGATGGTGTT
TTTGAGGTGC
TCCAGTGGCT
TCTGTGTCTA
TCAGCTGTCC
CTCCTGTTCA
GCTACTGACG
GGGTGGTGCG
TAACGGCAAA
AGCACCGCCG
GACATCAGCG
CTATCTCTGC
TCTCACTGCC
GTAAAACATG
GCAACTGCAG
TTCACTTACA
CCGCTTCTCA
ACCCGGTACG
CACCAGAAAA
TCATTGATAT
GGCCATGAAT
GGCGTTGGAT
GCCGGGCAAC
AGCCCGCATT
ATGGGCGTTG
GCCTCAACAC
GATTTTACGT
CACTTAAAAA
ACTCAGGCCG
CAGTCGGTAA
CCTCGCGCAT
ACAGCCGGGC
AGTGACGTCA
TCGTCTGCGC
GGAAATGGAC
GAACAGTGGG
GCTATGTCGG
GGCTAAATCG
CGCCAGCGCT
GGCTGTTTTA
CGCGTATGAC
AGTCTCCGGA
AGACGGTTGT
TGCGCACGTA
TTCGGTGAAC
GCACTATGGC
GACGCTGGGG
CGTCTTATGA
GCCTGCTGTC
ACCCTTTGAC
GTGGTGATAT
GGATGACGGA
TGGCTGGCCG
CTGTATGAAT
CCCGCCTGAA
GGGAAAGCTG
CACGTAATCA
GCAAGCGATA
TACGCAGCGA
ATTGAGCGGC
ATAACCTGAA
TCTGAGGCAG
CACCTGGCAC
GGCTGGGACG
GAAGTCGCTG
TCGTTCTCAA
AATCGGTGGA
GCTGCATGAC
AAAGTCATCG
GGCATTATCT
GAACATAAAA
CACTATCAAT
AAGTTGGAGT
CATTACCCAA
TTATGATAGA
ATTTACAAGC
TATAAGGTTA
TTGTCCTGGG
TTTCAAGCAT
TAGTCCATGC
AAGTTTTTAT
GCTTTGCCCA
TTCTATAGAT
ATATTGATAA
GCGCGCTGCC
TATGCCTTGC
CCCCTGAAAT
CCTTACATAC
GGCGATATCT
TCTATATAAA
AGATATATTA
TCTTATCAGT
ATTGTCAATA
TATTCAAGGC
AATCTGCCTC
CTCATCCTCT
TCATCCTCTT
CGTCTTGGTA
GCTTTTTAAA
TATGGCGCTT
CATAGAGTAA
TTCTGTAAAG
GTCCAATTCT
CGTTTTCATA
CCTCGGTATA
ATCTTACCTA
TCACCTCAAA
TGGTTCGCTG
GGTTTATCGC
ACCCCCGAAC
ACGAGCACGG
CACCCGCGAC
CACTATGCCA
AGAATGCCCA
AGGTAAAAAT
TGCCGGCCCC
GCCATGAAGT
CCGTGAATGC
CCCGACGGCC
GAAGTGAAGG
GCAGGCCGCC
ACCCAGGCCG
CCGCCCTCAC
TGCCCGGCAC
CTGGTCGCTG
AATGTCGATG
CCAGCACCTG
CGGCACGTCA
ATGCTTCCGG
GCGTCGCGCT
CGGGCTGATC
GCCCATCCCG
TTACTGCCCC
GATCCCGGCA
ATGGCAAGGA
CTGCCAGCGC
TGCCATTTTT
GGGGTGAGGC
CGTTCGCGGC
CGAGGGGCGC
AGCCCCTGGG
GGGATGGGAG
GCCCGCGTTA
GCGGGCCGGG
AGGGTTCGAG
AAGGGGGGGC
ACCCCCCTTC
GGCGTGCGCG
GTCACGCGCA
CAGGGCGCAG
CCCTGGTTAA
AAACAAGGTT
TATAAATATT
GGTTTAAAAG
CAGGTTAAAA
GACAGGTTAG
CGGTGGCCGA
AAAACGGGCG
GAAACCCTTG
CAAATGCTGG
ATTTTCTGCC
TGTGGACAGC
CCCTCAAATG
TCAATAGGTG
CGCCCCTCAT
CTGTCAGCAC
TCTGCCCCTC
AAGTGTCAAG
GATCGCGCCC
CTCATCTGTC
AGTAGTCGCG
CCCCTCAAGT
GTCAATACCG
CAGGGCACTT
ATCCCCAGGC
TTGTCCACAT
CATCTGTGGG
AAACTCGCGT
AAAATCAGGC
GTTTTCGCCG
ATTTGCGAGG
CTGGCCAGCT
CCACGTCGCC
GGCCGAAATC
GAGCCTGCCC
CTCATCTGTC
AACGCCGCGC
CGGGTGAGTC
GGCCCCTCAA
GTGTCAACGT
CCGCCCCTCA
TCTGTCAGTG
AGGGCCAAGT
TTTCCGCGAG
GTATCCACAA
CGCCGGCGGC
CGCGGTGTCT
CGCACACGGC
TTCGACGGCG
TTTCTGGCGC
GTTTGCAGGG
CCATAGACGG
CCGCCAGCCC
AGCGGCGAGG
GCAACCAGCC
CGG