名称: | pST1374-NLS-FLAG-Linker-Cas9 |
---|---|
别称: | Plasmid#44758 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | Blast |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pST1374 |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AGCGCCCAAT
ACGCAAACCG
CCTCTCCCCG
CGCGTTGGCC
GATTCATTAA
TGCAGCTGGC
ACGACAGGTT
TCCCGACTGG
AAAGCGGGCA
GTGAGCGCAA
CGCAATTAAT
GTGAGTTAGC
TCACTCATTA
GGCACCCCAG
GCTTTACACT
TTATGCTTCC
GGCTCGTATG
TTGTGTGGAA
TTGTGAGCGG
ATAACAATTT
CACACAGGAA
ACAGCTATGA
CCATGATTAC
GCCAAGCTCT
AGCTAGAGGT
CGACGGTATA
CAGACATGAT
AAGATACATT
GATGAGTTTG
GACAAACCAC
AACTAGAATG
CAGTGAAAAA
AATGCTTTAT
TTGTGAAATT
TGTGATGCTA
TTGCTTTATT
TGTAACCATT
ATAAGCTGCA
ATAAACAAGT
TGGGGTGGGC
GAAGAACTCC
AGCATGAGAT
CCCCGCGCTG
GAGGATCATC
CAGCCGGCGT
CCCGGAAAAC
GATTCCGAAG
CCCAACCTTT
CATAGAAGGC
GGCGGTGGAA
TCGAAATCTC
GTAGCACGTG
TCAGTCCTGC
TCCTCGGCCA
CGAAGTGCTT
AGCCCTCCCA
CACATAACCA
GAGGGCAGCA
ATTCACGAAT
CCCAACTGCC
GTCGGCTGTC
CATCACTGTC
CTTCACTATG
GCTTTGATCC
CAGGATGCAG
ATCGAGAAGC
ACCTGTCGGC
ACCGTCCGCA
GGGGCTCAAG
ATGCCCCTGT
TCTCATTTCC
GATCGCGACG
ATACAAGTCA
GGTTGCCAGC
TGCCGCAGCA
GCAGCAGTGC
CCAGCACCAC
GAGTTCTGCA
CAAGGTCCCC
CAGTAAAATG
ATATACATTG
ACACCAGTGA
AGATGCGGCC
GTCGCTAGAG
AGAGCTGCGC
TGGCGACGCT
GTAGTCTTCA
GAGATGGGGA
TGCTGTTGAT
TGTAGCCGTT
GCTCTTTCAA
TGAGGGTGGA
TTCTTCTTGA
GACAAAGGCT
TGGCCATGGT
TTAGTTCCTC
ACCTTGTCGT
ATTATACTAT
GCCGATATAC
TATGCCGATG
ATTAATTGTC
AACACGTGCT
GATCAGATCC
GAAAATGGAT
ATACAAGCTC
CCGGGAGCTT
TTTGCAAAAG
CCTAGGCCTC
CAAAAAAGCC
TCCTCACTAC
TTCTGGAATA
GCTCAGAGGC
AGAGGCGGCC
TCGGCCTCTG
CATAAATAAA
AAAAATTAGT
CAGCCATGGG
GCGGAGAATG
GGCGGAACTG
GGCGGAGTTA
GGGGCGGGAT
GGGCGGAGTT
AGGGGCGGGA
CTATGGTTGC
TGACTAATTG
AGATGCATGC
TTTGCATACT
TCTGCCTGCT
GGGGAGCCTG
GGGACTTTCC
ACACCTGGTT
GCTGACTAAT
TGAGATGCAT
GCTTTGCATA
CTTCTGCCTG
CTGGGGAGCC
TGGGGACTTT
CCACACCCTA
ACTGACACAC
ATTCCACAGA
ATTAATTCGC
GTTAAATTTT
TGTTAAATCA
GCTCATTTTT
TAACCAATAG
GCCGAAATCG
GCAAAATCCC
TTATAAATCA
AAAGAATAGA
CCGAGATAGG
GTTGAGTGTT
GTTCCAGTTT
GGAACAAGAG
TCCACTATTA
AAGAACGTGG
ACTCCAACGT
CAAAGGGCGA
AAAACCGTCT
ATCAGGGCGA
TGGCCCACTA
CGTGAACCAT
CACCCTAATC
AAGTTTTTTG
GGGTCGAGGT
GCCGTAAAGC
ACTAAATCGG
AACCCTAAAG
GGAGCCCCCG
ATTTAGAGCT
TGACGGGGAA
AGCCGGCGAA
CGTGGCGAGA
AAGGAAGGGA
AGAAAGCGAA
AGGAGCGGGC
GCTAGGGCGC
TGGCAAGTGT
AGCGGTCACG
CTGCGCGTAA
CCACCACACC
CGCCGCGCTT
AATGCGCCGC
TACAGGGCGC
GTGGGGATAC
CCCCTAGAGC
CCCAGCTGGT
TCTTTCCGCC
TCAGAAGCCA
TAGAGCCCAC
CGCATCCCCA
GCATGCCTGC
TATTGTCTTC
CCAATCCTCC
CCCTTGCTGT
CCTGCCCCAC
CCCACCCCCC
AGAATAGAAT
GACACCTACT
CAGACAATGC
GATGCAATTT
CCTCATTTTA
TTAGGAAAGG
ACAGTGGGAG
TGGCACCTTC
CAGGGTCAAG
GAAGGCACGG
GGGAGGGGCA
AACAACAGAT
GGCTGGCAAC
TAGAAGGCAC
AGTCGAGGCT
GATCAGCGGG
TTTAAACTCA
ATGGTGATGG
TGATGATGAC
CGGTACGCGT
AGAATCGAGA
CCGAGGAGAG
GGTTAGGGAT
AGGCTTACCT
TCGAAGGGCC
CCTAGTCGCC
GCCGAGCTGA
GACAGGTCGA
TGCGAGTCTC
GTACAGTCCG
GTAATTGACT
GGTGTATCAG
GGTGGCATCG
AGAACTTCTT
TGGTAGAGGT
GTACCGCTTC
CTGTCAATAG
TTGTATCGAA
ATACTTGAAG
GCAGCAGGAG
CGCCCAGATT
AGTCAGAGTA
AAGAGGTGGA
TAATATTCTC
TGCTTGTTCG
CGAATTGGCT
TGTCCCTGTG
CTTATTATAT
GCGCTCAGCA
CTTTATCGAG
GTTTGCATCG
GCCAGAATAA
CCCGCTTGCT
GAACTCGCTA
ATCTGTTCAA
TGATTTCGTC
CAGGTAGTGT
TTATGTTGCT
CAACAAAGAG
TTGCTTCTGC
TCATTGTCTT
CAGGGCTACC
TTTGAGTTTC
TCGTAGTGGG
AGGCCAGATA
CAGGAAGTTC
ACGTATTTGG
AGGGCAGAGC
CAGCTCGTTT
CCTTTCTGCA
GCTCTCCGGC
GGAGGCCAGC
ATCCGCTTCC
TACCATTCTC
CAGCTCAAAG
AGAGAGTACT
TGGGCAGTTT
GATGATGAGA
TCTTTCTTCA
CTTCTTTATA
GCCCTTAGCT
TCCAGGAAAT
CGATTGGATT
CTTCTCGAAG
CTGGATCTCT
CCATAATAGT
AATTCCGAGC
AGCTCCTTAA
CAGACTTGAG
TTTCTTGGAC
TTGCCTTTCT
CCACTTTTGC
CACGACCAGA
ACGGAATAAG
CCACTGTAGG
GGAATCGAAA
CCGCCATACT
TCTTTGGGTC
CCAATCTTTC
TTCCTGGCGA
TCAGCTTGTC
AGAGTTCCGC
TTTGGCAGGA
TGCTCTCCTT
TGAGAATCCG
CCGGTCTGCA
CTTCGGTCTT
CTTCACGATA
TTGACTTGTG
GCATGGACAG
CACCTTCCGC
ACAGTTGCGA
AGTCCCTGCC
TTTATCCCAC
ACGATTTCTC
CTGTTTCTCC
GTTTGTTTCG
ATCAGTGGGC
GCTTCCGGAT
TTCGCCGTTA
GCCAGGGTTA
TCTCAGTCTT
AAAGAAATTC
ATGATATTAG
AGTAGAAGAA
GTACTTGGCG
GTGGCTTTGC
CAATCTCTTG
CTCAGACTTT
GCTATCATCT
TCCTCACATC
GTAGACTTTA
TAGTCACCGT
ACACGAACTC
AGACTCCAGT
TTAGGGTATT
TCTTGATCAG
GGCGGTGCCA
ACGACAGCAT
TGAGATAGGC
ATCGTGAGCA
TGATGGTAAT
TGTTTATTTC
GCGAACTTTG
TAGAATTGAA
AGTCCTTCCG
GAAGTCAGAC
ACCAGCTTGC
TCTTCAGAGT
TATCACTTTC
ACTTCCCTGA
TCAGCTTATC
GTTCTCATCG
TACTTAGTGT
TCATCCTAGA
GTCGAGGATT
TGTGCCACGT
GTTTGGTAAT
CTGGCGTGTT
TCGACCAGTT
GCCTCTTAAT
AAAGCCGGCC
TTGTCGAGTT
CGCTCAGACC
TCCTCTTTCT
GCCTTAGTCA
GATTGTCAAA
CTTCCGCTGG
GTGATCAGTT
TGGCGTTGAG
GAGCTGCCGC
CAGTAATTCT
TCATCTTCTT
GACCACCTCT
TCTGATGGAA
CATTGTCAGA
CTTACCGCGA
TTCTTATCGG
ATCTGGTCAG
CACCTTGTTG
TCAATGGAGT
CATCTTTGAG
GAAGGACTGT
GGAACAATAT
GGTCCACGTC
ATAATCGGAC
AGCCGGTTGA
TGTCGAGTTC
CTGGTCAACG
TACATGTCCC
GCCCGTTCTG
GAGGTAGTAC
AGGTAGAGTT
TCTCGTTCTG
GAGCTGTGTA
TTCTCCACAG
GGTGCTCCTT
CAGTATCTGA
GATCCCAGCT
CCTTAATTCC
CTCTTCGATT
CTTTTCATCC
GTTCCCGAGA
GTTCTTCTGG
CCCTTCTGGG
TGGTTTGGTT
CTCCCTTGCC
ATTTCGATAA
CGATATTCTC
TGGCTTGTGC
CGACCCATCA
CTTTGACGAG
TTCGTCCACG
ACCTTGACTG
TCTGCAGTAT
TCCCTTCTTT
ATGGCGGGTG
ATCCAGCGAG
GTTGGCGATG
TGCTCGTGCA
GGCTGTCGCC
TTGACCGCTC
ACCTGTGCCT
TCTGGATGTC
CTCTTTAAAT
GTCAGAGAGT
CATCGTGAAT
CAGTTGCATG
AAGTTCCTGT
TAGCGAATCC
GTCGGATTTC
AGGAAATCCA
GGATGGTCTT
TCCGCTCTGT
TTGTCGCGGA
TGCCGTTTAT
GAGTTTCCTG
GAGAGTCTAC
CCCACCCAGT
GTAGCGCCGC
CGCTTGAGCT
GTTTCATGAC
TTTATCGTCA
AACAGATGGG
CATAGGTCTT
CAGGCGCTCT
TCGATCATCT
CTCTATCCTC
GAACAGAGTC
AGGGTCAGCA
CGATATCTTC
CAGGATGTCC
TCGTTCTCCT
CATTATCGAG
GAAATCTTTG
TCTTTGATTA
TCTTCAGCAG
ATCATGGTAA
GTGCCCAGGC
TGGCATTAAA
GCGGTCCTCC
ACGCCAGAGA
TTTCCACTGA
GTCAAAGCAT
TCGATCTTCT
TAAAGTAATC
CTCCTTGAGC
TGCTTCACTG
TCACCTTTCT
ATTAGTCTTG
AAGAGCAGAT
CAACGATAGC
CTTCTTCTGC
TCTCCGGACA
GGAAGGCAGG
CTTCCTCATG
CCCTCGGTCA
CGTACTTCAC
TTTAGTCAGC
TCGTTATAGA
CGGTGAAATA
CTCGTACAGG
AGTGAATGTT
TGGGCAGGAC
CTTCTCGTTG
GGCAGGTTCT
TATCGAAATT
GGTCATCCGT
TCGATGAATG
ACTGGGCGCT
TGCGCCCTTA
TCCACGACCT
CTTCGAAGTT
CCAAGGAGTA
ATTGTCTCCT
CAGATTTCCT
GGTCATCCAA
GCGAAGCGGG
AGTTGCCTCT
AGCCAGAGGG
CCGACGTAAT
AAGGGATCCT
GAAGGTCAGG
ATCTTCTCTA
TCTTCTCCCG
GTTATCCTTC
AGGAAAGGAT
AGAAATCCTC
CTGCCTGCGG
AGGATTGCAT
GCAGCTCTCC
CAGGTGTATC
TGATGTGGAA
TGGAGCCATT
ATCAAAGGTC
CTCTGCTTCC
TCAGCAGGTC
CTCCCTGTTC
AGCTTCACCA
GCAGCTCTTC
AGTACCGTCC
ATCTTCTCGA
GGATTGGTTT
GATGAACTTG
TAAAATTCTT
CCTGTGATGC
TCCGCCATCG
ATGTATCCGG
CATATCCATT
CTTGCTCTGG
TCGAAGAATA
TCTCTTTGTA
CTTCTCTGGC
AGCTGTTGCC
TCACGAGGGC
TTTGAGCAGA
GTCAGGTCTT
GATGGTGTTC
ATCATAGCGT
TTTATCATGG
AGGCGCTCAG
AGGTGCTTTG
GTGATCTCAG
TGTTGACCCG
GAGTATGTCG
CTCAGCAGAA
TGGCGTCGGA
GAGATTCTTA
GCAGCCAGAA
AGAGATCGGC
GTACTGGTCG
CCTATCTGTG
CGAGCAGGTT
GTCCAGATCA
TCGTCATAGG
TGTCCTTGGA
GAGCTGGAGT
TTAGCATCTT
CGGCCAGATC
AAAATTGGAC
TTGAAGTTAG
GTGTCAGGCC
CAGGCTCAGG
GCGATGAGGT
TCCCAAACAG
GCCGTTCTTC
TTTTCTCCTG
GCAGTTGGGC
AATCAGATTC
TCCAGTCTGC
GTGACTTGGA
CAGCCGAGCG
GACAGAATAG
CCTTGGCATC
CACACCAGAA
GCGTTTATGG
GATTCTCCTC
GAACAGTTGG
TTATATGTCT
GCACCAGTTG
AATGAAGAGT
TTATCCACAT
CGGAATTATC
GGGATTCAGG
TCGCCCTCGA
TCAGAAAGTG
TCCTCTAAAC
TTTATCATAT
GAGCCAGGGC
CAGATAGATG
AGCCTCAGGT
CTGCTTTATC
GGTGCTGTCC
ACCAGCTTCT
TCCTCAGATG
GTAGATTGTA
GGATACTTTT
CATGGTAAGC
CACCTCATCC
ACGATATTTC
CGAATATAGG
GTGCCTCTCG
TGTTTCTTAT
CCTCCTCCAC
CAGAAAGCTC
TCTTCCAGGC
GGTGAAAGAA
GGAGTCGTCC
ACCTTAGCCA
TTTCGTTGCT
AAAGATCTCT
TGCAGATAAC
ATATCCGATT
CTTGCGGCGG
GTGTATCTCC
GCCTTGCGGT
CCGCTTCAGC
CGAGTAGCTT
CAGCGGTTTC
ACCGGAGTCG
AAGAGGAGTG
CTCCGATCAG
GTTCTTCTTG
ATTGAATGGC
GGTCAGTATT
ACCCAGCACC
TTGAATTTCT
TGCTTGGCAC
CTTATACTCG
TCGGTTATGA
CGGCCCAGCC
AACGGAGTTT
GTCCCGATAT
CCAGTCCAAT
AGAGTATTTC
TTGTCTCTAG
TGTGGGTCCG
CTGGTGTCTG
GTCAGAGCAC
CAGACTGAGA
GAAAGATTTA
CCACACTCTG
GGCATTTGTA
TGGCTTCTCG
CCGGGTTCCA
GTCTAGATTT
ATCGTCGTCA
TCCTTGTAGT
CAGCGGCCGC
CACCTTCCTC
TTTTTCTTAG
GTCCCATGGT
GCTAGCCAGC
TTGGGTCTCC
CTATAGTGAG
TCGTATTAAT
TTCGATAAGC
CAGTAAGCAG
TGGGTTCTCT
AGTTAGCCAG
AGAGCTCTGC
TTATATAGAC
CTCCCACCGT
ACACGCCTAC
CGCCCATTTG
CGTCAATGGG
GCGGAGTTGT
TACGACATTT
TGGAAAGTCC
CGTTGATTTT
GGTGCCAAAA
CAAACTCCCA
TTGACGTCAA
TGGGGTGGAG
ACTTGGAAAT
CCCCGTGAGT
CAAACCGCTA
TCCACGCCCA
TTGATGTACT
GCCAAAACCG
CATCACCATG
GTAATAGCGA
TGACTAATAC
GTAGATGTAC
TGCCAAGTAG
GAAAGTCCCA
TAAGGTCATG
TACTGGGCAT
AATGCCAGGC
GGGCCATTTA
CCGTCATTGA
CGTCAATAGG
GGGCGTACTT
GGCATATGAT
ACACTTGATG
TACTGCCAAG
TGGGCAGTTT
ACCGTAAATA
CTCCACCCAT
TGACGTCAAT
GGAAAGTCCC
TATTGGCGTT
ACTATGGGAA
CATACGTCAT
TATTGACGTC
AATGGGCGGG
GGTCGTTGGG
CGGTCAGCCA
GGCGGGCCAT
TTACCGTAAG
TTATGTAACG
CGGAACTCCA
TATATGGGCT
ATGAACTAAT
GACCCCGTAA
TTGATTACTA
TTAATAACTA
GTCAATAATC
AATGTCAACG
CGTATATCTG
GCCCGTACAT
CGCGAAGCAG
CGCAAAACGC
CTAACCCTAA
GCAGATTCTT
CATGCAATTG
TCGGTCAAGC
CTTGCCTTGT
TGTAGCTTAA
ATTTTGCTCG
CGCACTACTC
AGCGACCTCC
AACACACAAG
CAGGGAGCAG
ATACTGGCTT
AACTATGCGG
CATCAGAGCA
GATTGTACTG
AGAGTGCACC
ATAGGGGATC
GGGAGATCTC
CCGATCCGTC
GACGTCAGGT
GGCACTTTTC
GGGGAAATGT
GCGCGGAACC
CCTATTTGTT
TATTTTTCTA
AATACATTCA
AATATGTATC
CGCTCATGAG
ACAATAACCC
TGATAAATGC
TTCAATAATA
TTGAAAAAGG
AAGAGTATGA
GTATTCAACA
TTTCCGTGTC
GCCCTTATTC
CCTTTTTTGC
GGCATTTTGC
CTTCCTGTTT
TTGCTCACCC
AGAAACGCTG
GTGAAAGTAA
AAGATGCTGA
AGATCAGTTG
GGTGCACGAG
TGGGTTACAT
CGAACTGGAT
CTCAACAGCG
GTAAGATCCT
TGAGAGTTTT
CGCCCCGAAG
AACGTTTTCC
AATGATGAGC
ACTTTTAAAG
TTCTGCTATG
TGGCGCGGTA
TTATCCCGTA
TTGACGCCGG
GCAAGAGCAA
CTCGGTCGCC
GCATACACTA
TTCTCAGAAT
GACTTGGTTG
AGTACTCACC
AGTCACAGAA
AAGCATCTTA
CGGATGGCAT
GACAGTAAGA
GAATTATGCA
GTGCTGCCAT
AACCATGAGT
GATAACACTG
CGGCCAACTT
ACTTCTGACA
ACGATCGGAG
GACCGAAGGA
GCTAACCGCT
TTTTTGCACA
ACATGGGGGA
TCATGTAACT
CGCCTTGATC
GTTGGGAACC
GGAGCTGAAT
GAAGCCATAC
CAAACGACGA
GCGTGACACC
ACGATGCCTG
TAGCAATGGC
AACAACGTTG
CGCAAACTAT
TAACTGGCGA
ACTACTTACT
CTAGCTTCCC
GGCAACAATT
AATAGACTGG
ATGGAGGCGG
ATAAAGTTGC
AGGACCACTT
CTGCGCTCGG
CCCTTCCGGC
TGGCTGGTTT
ATTGCTGATA
AATCTGGAGC
CGGTGAGCGT
GGGTCTCGCG
GTATCATTGC
AGCACTGGGG
CCAGATGGTA
AGCCCTCCCG
TATCGTAGTT
ATCTACACGA
CGGGGAGTCA
GGCAACTATG
GATGAACGAA
ATAGACAGAT
CGCTGAGATA
GGTGCCTCAC
TGATTAAGCA
TTGGTAACTG
TCAGACCAAG
TTTACTCATA
TATACTTTAG
ATTGATTTAA
AACTTCATTT
TTAATTTAAA
AGGATCTAGG
TGAAGATCCT
TTTTGATAAT
CTCATGACCA
AAATCCCTTA
ACGTGAGTTT
TCGTTCCACT
GAGCGTCAGA
CCCCGTAGAA
AAGATCAAAG
GATCTTCTTG
AGATCCTTTT
TTTCTGCGCG
TAATCTGCTG
CTTGCAAACA
AAAAAACCAC
CGCTACCAGC
GGTGGTTTGT
TTGCCGGATC
AAGAGCTACC
AACTCTTTTT
CCGAAGGTAA
CTGGCTTCAG
CAGAGCGCAG
ATACCAAATA
CTGTTCTTCT
AGTGTAGCCG
TAGTTAGGCC
ACCACTTCAA
GAACTCTGTA
GCACCGCCTA
CATACCTCGC
TCTGCTAATC
CTGTTACCAG
TGGCTGCTGC
CAGTGGCGAT
AAGTCGTGTC
TTACCGGGTT
GGACTCAAGA
CGATAGTTAC
CGGATAAGGC
GCAGCGGTCG
GGCTGAACGG
GGGGTTCGTG
CACACAGCCC
AGCTTGGAGC
GAACGACCTA
CACCGAACTG
AGATACCTAC
AGCGTGAGCT
ATGAGAAAGC
GCCACGCTTC
CCGAAGGGAG
AAAGGCGGAC
AGGTATCCGG
TAAGCGGCAG
GGTCGGAACA
GGAGAGCGCA
CGAGGGAGCT
TCCAGGGGGA
AACGCCTGGT
ATCTTTATAG
TCCTGTCGGG
TTTCGCCACC
TCTGACTTGA
GCGTCGATTT
TTGTGATGCT
CGTCAGGGGG
GCGGAGCCTA
TGGAAAAACG
CCAGCAACGC
GGCCTTTTTA
CGGTTCCTGG
CCTTTTGCTG
GCCTTTTGCT
CACATGTTCT
TTCCTGCGTT
ATCCCCTGAT
TCTGTGGATA
ACCGTATTAC
CGCCTTTGAG
TGAGCTGATA
CCGCTCGCCG
CAGCCGAACG
ACCGAGCGCA
GCGAGTCAGT
GAGCGAGGAA
GCGGAAG