名称: | pC0046-EF1a-PspCas13b-NES-HIV |
---|---|
别称: | Plasmid#103862 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GAACAACACT
CAACCCTATC
TCGGTCTATT
CTTTTGATTT
ATAAGGGATT
TTGCCGATTT
CGGCCTATTG
GTTAAAAAAT
GAGCTGATTT
AACAAAAATT
TAACGCGAAT
TAATTCTGTG
GAATGTGTGT
CAGTTAGGGT
GTGGAAAGTC
CCCAGGCTCC
CCAGCAGGCA
GAAGTATGCA
AAGCATGCAT
CTCAATTAGT
CAGCAACCAG
GTGTGGAAAG
TCCCCAGGCT
CCCCAGCAGG
CAGAAGTATG
CAAAGCATGC
ATCTCAATTA
GTCAGCAACC
ATAGTCCCGC
CCCTAACTCC
GCCCATCCCG
CCCCTAACTC
CGCCCAGTTC
CGCCCATTCT
CCGCCCCATG
GCTGACTAAT
TTTTTTTATT
TATGCAGAGG
CCGAGGCCGC
CTCTGCCTCT
GAGCTATTCC
AGAAGTAGTG
AGGAGGCTTT
TTTGGAGGCC
TAGGCTTTTG
CAAAAAGCTC
CCGGGAGCTT
GTATATCCAT
TTTCGGATCT
GATCAGCACG
TGTTGACAAT
TAATCATCGG
CATAGTATAT
CGGCATAGTA
TAATACGACA
AGGTGAGGAA
CTAAACCATG
GCCAAGTTGA
CCAGTGCCGT
TCCGGTGCTC
ACCGCGCGCG
ACGTCGCCGG
AGCGGTCGAG
TTCTGGACCG
ACCGGCTCGG
GTTCTCCCGG
GACTTCGTGG
AGGACGACTT
CGCCGGTGTG
GTCCGGGACG
ACGTGACCCT
GTTCATCAGC
GCGGTCCAGG
ACCAGGTGGT
GCCGGACAAC
ACCCTGGCCT
GGGTGTGGGT
GCGCGGCCTG
GACGAGCTGT
ACGCCGAGTG
GTCGGAGGTC
GTGTCCACGA
ACTTCCGGGA
CGCCTCCGGG
CCGGCCATGA
CCGAGATCGG
CGAGCAGCCG
TGGGGGCGGG
AGTTCGCCCT
GCGCGACCCG
GCCGGCAACT
GCGTGCACTT
CGTGGCCGAG
GAGCAGGACT
GACACGTGCT
ACGAGATTTC
GATTCCACCG
CCGCCTTCTA
TGAAAGGTTG
GGCTTCGGAA
TCGTTTTCCG
GGACGCCGGC
TGGATGATCC
TCCAGCGCGG
GGATCTCATG
CTGGAGTTCT
TCGCCCACCC
CAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGTATACCGT
CGACCTCTAG
CTAGAGCTTG
GCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTGACGTCG
ACGGATCGGG
AGATCTCCCG
ATCCCCTATG
GTGCACTCTC
AGTACAATCT
GCTCTGATGC
CGCATAGTTA
AGCCAGTATC
TGCTCCCTGC
TTGTGTGTTG
GAGGTCGCTG
AGTAGTGCGC
GAGCAAAATT
TAAGCTACAA
CAAGGCAAGG
CTTGACCGAC
AATTGCATGA
AGAATCTGCT
TAGGGTTAGG
CGTTTTGCGC
TGCTTCGCGA
TGTACGGGCC
AGATATACGC
GTTGACATTG
ATTATTGACT
AGTTATTAAT
AGTAATCAAT
TACGGGGTCA
TTAGTTCATA
GCCCATATAT
GGAGTTCCGC
GTTACATAAC
TTACGGTAAA
TGGCCCGCCT
GGCTGACCGC
CCAACGACCC
CCGCCCATTG
ACGTCAATAA
TGACGTATGT
TCCCATAGTA
ACGCCAATAG
GGACTTTCCA
TTGACGTCAA
TGGGTGGAGT
ATTTACGGTA
AACTGCCCAC
TTGGCAGTAC
ATCAAGTGTA
TCATATGCCA
AGTACGCCCC
CTATTGACGT
CAATGACGGT
AAATGGCCCG
CCTGGCATTA
TGCCCAGTAC
ATGACCTTAT
GGGACTTTCC
TACTTGGCAG
TACATCTACG
TATTAGTCAT
CGCTATTACC
ATGGTGATGC
GGTTTTGGCA
GTACATCAAT
GGGCGTGGAT
AGCGGTTTGA
CTCACGGGGA
TTTCCAAGTC
TCCACCCCAT
TGACGTCAAT
GGGAGTTTGT
TTTGGCACCA
AAATCAACGG
GACTTTCCAA
AATGTCGTAA
CAACTCCGCC
CCATTGACGC
AAATGGGCGG
TAGGCGTGTA
CGGTGGGAGG
TCTATATAAG
CAGCGCGTTT
TGCCTGTACT
GGGTCTCTCT
GGTTAGACCA
GATCTGAGCC
TGGGAGCTCT
CTGGCTAACT
AGGGAACCCA
CTGCTTAAGC
CTCAATAAAG
CTTGCCTTGA
GTGCTTCAAG
TAGTGTGTGC
CCGTCTGTTG
TGTGACTCTG
GTAACTAGAG
ATCCCTCAGA
CCCTTTTAGT
CAGTGTGGAA
AATCTCTAGC
AGTGGCGCCC
GAACAGGGAC
TTGAAAGCGA
AAGGGAAACC
AGAGGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCGCTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCG
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTTGG
AATCACACGA
CCTGGATGGA
GTGGGACAGA
GAAATTAACA
ATTACACAAG
CTTAATACAC
TCCTTAATTG
AAGAATCGCA
AAACCAGCAA
GAAAAGAATG
AACAAGAATT
ATTGGAATTA
GATAAATGGG
CAAGTTTGTG
GAATTGGTTT
AACATAACAA
ATTGGCTGTG
GTATATAAAA
TTATTCATAA
TGATAGTAGG
AGGCTTGGTA
GGTTTAAGAA
TAGTTTTTGC
TGTACTTTCT
ATAGTGAATA
GAGTTAGGCA
GGGATATTCA
CCATTATCGT
TTCAGACCCA
CCTCCCAACC
CCGAGGGGAC
CCGACAGGCC
CGAAGGAATA
GAAGAAGAAG
GTGGAGAGAG
AGACAGAGAC
AGATCCATTC
GATTAGTGAA
CGGATCGGCA
CTGCGTGCGC
CAATTCTGCA
GACAAATGGC
AGTATTCATC
CACAATTTTA
AAAGAAAAGG
GGGGATTGGG
GGGTACAGTG
CAGGGGAAAG
AATAGTAGAC
ATAATAGCAA
CAGACATACA
AACTAAAGAA
TTACAAAAAC
AAATTACAAA
AATTCAAAAT
TTTCGGGTTT
ATTACAGGGA
CAGCAGAGAT
CCAGTTTGGT
TAATTAATGC
AAAGATGGAT
AAAGTTTTAA
ACAGAGAGGA
ATCTTTGCAG
CTAATGGACC
TTCTAGGTCT
TGAAAGGAGT
GGGAATTGGC
TCCGGTGCCC
GTCAGTGGGC
AGAGCGCACA
TCGCCCACAG
TCCCCGAGAA
GTTGGGGGGA
GGGGTCGGCA
ATTGAACCGG
TGCCTAGAGA
AGGTGGCGCG
GGGTAAACTG
GGAAAGTGAT
GTCGTGTACT
GGCTCCGCCT
TTTTCCCGAG
GGTGGGGGAG
AACCGTATAT
AAGTGCAGTA
GTCGCCGTGA
ACGTTCTTTT
TCGCAACGGG
TTTGCCGCCA
GAACACAGGT
AAGTGCCGTG
TGTGGTTCCC
GCGGGCCTGG
CCTCTTTACG
GGTTATGGCC
CTTGCGTGCC
TTGAATTACT
TCCACCTGGC
TGCAGTACGT
GATTCTTGAT
CCCGAGCTTC
GGGTTGGAAG
TGGGTGGGAG
AGTTCGAGGC
CTTGCGCTTA
AGGAGCCCCT
TCGCCTCGTG
CTTGAGTTGA
GGCCTGGCCT
GGGCGCTGGG
GCCGCCGCGT
GCGAATCTGG
TGGCACCTTC
GCGCCTGTCT
CGCTGCTTTC
GATAAGTCTC
TAGCCATTTA
AAATTTTTGA
TGACCTGCTG
CGACGCTTTT
TTTCTGGCAA
GATAGTCTTG
TAAATGCGGG
CCAAGATCTG
CACACTGGTA
TTTCGGTTTT
TGGGGCCGCG
GGCGGCGACG
GGGCCCGTGC
GTCCCAGCGC
ACATGTTCGG
CGAGGCGGGG
CCTGCGAGCG
CGGCCACCGA
GAATCGGACG
GGGGTAGTCT
CAAGCTGGCC
GGCCTGCTCT
GGTGCCTGGC
CTCGCGCCGC
CGTGTATCGC
CCCGCCCTGG
GCGGCAAGGC
TGGCCCGGTC
GGCACCAGTT
GCGTGAGCGG
AAAGATGGCC
GCTTCCCGGC
CCTGCTGCAG
GGAGCTCAAA
ATGGAGGACG
CGGCGCTCGG
GAGAGCGGGC
GGGTGAGTCA
CCCACACAAA
GGAAAAGGGC
CTTTCCGTCC
TCAGCCGTCG
CTTCATGTGA
CTCCACGGAG
TACCGGGCGC
CGTCCAGGCA
CCTCGATTAG
TTCTCGAGCT
TTTGGAGTAC
GTCGTCTTTA
GGTTGGGGGG
AGGGGTTTTA
TGCGATGGAG
TTTCCCCACA
CTGAGTGGGT
GGAGACTGAA
GTTAGGCCAG
CTTGGCACTT
GATGTAATTC
TCCTTGGAAT
TTGCCCTTTT
TGAGTTTGGA
TCTTGGTTCA
TTCTCAAGCC
TCAGACAGTG
GTTCAAAGTT
TTTTTCTTCC
ATTTCAGGTG
TCGTGACGTA
CGGCCACCAT
GAACATCCCC
GCTCTGGTGG
AAAACCAGAA
GAAGTACTTT
GGCACCTACA
GCGTGATGGC
CATGCTGAAC
GCTCAGACCG
TGCTGGACCA
CATCCAGAAG
GTGGCCGATA
TTGAGGGCGA
GCAGAACGAG
AACAACGAGA
ATCTGTGGTT
TCACCCCGTG
ATGAGCCACC
TGTACAACGC
CAAGAACGGC
TACGACAAGC
AGCCCGAGAA
AACCATGTTC
ATCATCGAGC
GGCTGCAGAG
CTACTTCCCA
TTCCTGAAGA
TCATGGCCGA
GAACCAGAGA
GAGTACAGCA
ACGGCAAGTA
CAAGCAGAAC
CGCGTGGAAG
TGAACAGCAA
CGACATCTTC
GAGGTGCTGA
AGCGCGCCTT
CGGCGTGCTG
AAGATGTACA
GGGACCTGAC
CAACCACTAC
AAGACCTACG
AGGAAAAGCT
GAACGACGGC
TGCGAGTTCC
TGACCAGCAC
AGAGCAACCT
CTGAGCGGCA
TGATCAACAA
CTACTACACA
GTGGCCCTGC
GGAACATGAA
CGAGAGATAC
GGCTACAAGA
CAGAGGACCT
GGCCTTCATC
CAGGACAAGC
GGTTCAAGTT
CGTGAAGGAC
GCCTACGGCA
AGAAAAAGTC
CCAAGTGAAT
ACCGGATTCT
TCCTGAGCCT
GCAGGACTAC
AACGGCGACA
CACAGAAGAA
GCTGCACCTG
AGCGGAGTGG
GAATCGCCCT
GCTGATCTGC
CTGTTCCTGG
ACAAGCAGTA
CATCAACATC
TTTCTGAGCA
GGCTGCCCAT
CTTCTCCAGC
TACAATGCCC
AGAGCGAGGA
ACGGCGGATC
ATCATCAGAT
CCTTCGGCAT
CAACAGCATC
AAGCTGCCCA
AGGACCGGAT
CCACAGCGAG
AAGTCCAACA
AGAGCGTGGC
CATGGATATG
CTCAACGAAG
TGAAGCGGTG
CCCCGACGAG
CTGTTCACAA
CACTGTCTGC
CGAGAAGCAG
TCCCGGTTCA
GAATCATCAG
CGACGACCAC
AATGAAGTGC
TGATGAAGCG
GAGCAGCGAC
AGATTCGTGC
CTCTGCTGCT
GCAGTATATC
GATTACGGCA
AGCTGTTCGA
CCACATCAGG
TTCCACGTGA
ACATGGGCAA
GCTGAGATAC
CTGCTGAAGG
CCGACAAGAC
CTGCATCGAC
GGCCAGACCA
GAGTCAGAGT
GATCGAGCAG
CCCCTGAACG
GCTTCGGCAG
ACTGGAAGAG
GCCGAGACAA
TGCGGAAGCA
AGAGAACGGC
ACCTTCGGCA
ACAGCGGCAT
CCGGATCAGA
GACTTCGAGA
ACATGAAGCG
GGACGACGCC
AATCCTGCCA
ACTATCCCTA
CATCGTGGAC
ACCTACACAC
ACTACATCCT
GGAAAACAAC
AAGGTCGAGA
TGTTTATCAA
CGACAAAGAG
GACAGCGCCC
CACTGCTGCC
CGTGATCGAG
GATGATAGAT
ACGTGGTCAA
GACAATCCCC
AGCTGCCGGA
TGAGCACCCT
GGAAATTCCA
GCCATGGCCT
TCCACATGTT
TCTGTTCGGC
AGCAAGAAAA
CCGAGAAGCT
GATCGTGGAC
GTGCACAACC
GGTACAAGAG
ACTGTTCCAG
GCCATGCAGA
AAGAAGAAGT
GACCGCCGAG
AATATCGCCA
GCTTCGGAAT
CGCCGAGAGC
GACCTGCCTC
AGAAGATCCT
GGATCTGATC
AGCGGCAATG
CCCACGGCAA
GGATGTGGAC
GCCTTCATCA
GACTGACCGT
GGACGACATG
CTGACCGACA
CCGAGCGGAG
AATCAAGAGA
TTCAAGGACG
ACCGGAAGTC
CATTCGGAGC
GCCGACAACA
AGATGGGAAA
GAGAGGCTTC
AAGCAGATCT
CCACAGGCAA
GCTGGCCGAC
TTCCTGGCCA
AGGACATCGT
GCTGTTTCAG
CCCAGCGTGA
ACGATGGCGA
GAACAAGATC
ACCGGCCTGA
ACTACCGGAT
CATGCAGAGC
GCCATTGCCG
TGTACGATAG
CGGCGACGAT
TACGAGGCCA
AGCAGCAGTT
CAAGCTGATG
TTCGAGAAGG
CCCGGCTGAT
CGGCAAGGGC
ACAACAGAGC
CTCATCCATT
TCTGTACAAG
GTGTTCGCCC
GCAGCATCCC
CGCCAATGCC
GTCGAGTTCT
ACGAGCGCTA
CCTGATCGAG
CGGAAGTTCT
ACCTGACCGG
CCTGTCCAAC
GAGATCAAGA
AAGGCAACAG
AGTGGATGTG
CCCTTCATCC
GGCGGGACCA
GAACAAGTGG
AAAACACCCG
CCATGAAGAC
CCTGGGCAGA
ATCTACAGCG
AGGATCTGCC
CGTGGAACTG
CCCAGACAGA
TGTTCGACAA
TGAGATCAAG
TCCCACCTGA
AGTCCCTGCC
ACAGATGGAA
GGCATCGACT
TCAACAATGC
CAACGTGACC
TATCTGATCG
CCGAGTACAT
GAAGAGAGTG
CTGGACGACG
ACTTCCAGAC
CTTCTACCAG
TGGAACCGCA
ACTACCGGTA
CATGGACATG
CTTAAGGGCG
AGTACGACAG
AAAGGGCTCC
CTGCAGCACT
GCTTCACCAG
CGTGGAAGAG
AGAGAAGGCC
TCTGGAAAGA
GCGGGCCTCC
AGAACAGAGC
GGTACAGAAA
GCAGGCCAGC
AACAAGATCC
GCAGCAACCG
GCAGATGAGA
AACGCCAGCA
GCGAAGAGAT
CGAGACAATC
CTGGATAAGC
GGCTGAGCAA
CAGCCGGAAC
GAGTACCAGA
AAAGCGAGAA
AGTGATCCGG
CGCTACAGAG
TGCAGGATGC
CCTGCTGTTT
CTGCTGGCCA
AAAAGACCCT
GACCGAACTG
GCCGATTTCG
ACGGCGAGAG
GTTCAAACTG
AAAGAAATCA
TGCCCGACGC
CGAGAAGGGA
ATCCTGAGCG
AGATCATGCC
CATGAGCTTC
ACCTTCGAGA
AAGGCGGCAA
GAAGTACACC
ATCACCAGCG
AGGGCATGAA
GCTGAAGAAC
TACGGCGACT
TCTTTGTGCT
GGCTAGCGAC
AAGAGGATCG
GCAACCTGCT
GGAACTCGTG
GGCAGCGACA
TCGTGTCCAA
AGAGGATATC
ATGGAAGAGT
TCAACAAATA
CGACCAGTGC
AGGCCCGAGA
TCAGCTCCAT
CGTGTTCAAC
CTGGAAAAGT
GGGCCTTCGA
CACATACCCC
GAGCTGTCTG
CCAGAGTGGA
CCGGGAAGAG
AAGGTGGACT
TCAAGAGCAT
CCTGAAAATC
CTGCTGAACA
ACAAGAACAT
CAACAAAGAG
CAGAGCGACA
TCCTGCGGAA
GATCCGGAAC
GCCTTCGATC
ACAACAATTA
CCCCGACAAA
GGCGTGGTGG
AAATCAAGGC
CCTGCCTGAG
ATCGCCATGA
GCATCAAGAA
GGCCTTTGGG
GAGTACGCCA
TCATGAAGGG
ATCCCTTCAA
CTGCCTCCAC
TTGAAAGACT
GACACTGGGT
AGTTCCTACC
CATACGATGT
TCCAGATTAC
GCTTATCCCT
ACGACGTGCC
TGATTATGCA
TACCCATATG
ATGTCCCCGA
CTATGCCTAA
GAATTCGATA
TCAAGCTTAT
CGATAATCAA
CCTCTGGATT
ACAAAATTTG
TGAAAGATTG
ACTGGTATTC
TTAACTATGT
TGCTCCTTTT
ACGCTATGTG
GATACGCTGC
TTTAATGCCT
TTGTATCATG
CTATTGCTTC
CCGTATGGCT
TTCATTTTCT
CCTCCTTGTA
TAAATCCTGG
TTGCTGTCTC
TTTATGAGGA
GTTGTGGCCC
GTTGTCAGGC
AACGTGGCGT
GGTGTGCACT
GTGTTTGCTG
ACGCAACCCC
CACTGGTTGG
GGCATTGCCA
CCACCTGTCA
GCTCCTTTCC
GGGACTTTCG
CTTTCCCCCT
CCCTATTGCC
ACGGCGGAAC
TCATCGCCGC
CTGCCTTGCC
CGCTGCTGGA
CAGGGGCTCG
GCTGTTGGGC
ACTGACAATT
CCGTGGTGTT
GTCGGGGAAA
TCATCGTCCT
TTCCTTGGCT
GCTCGCCTGT
GTTGCCACCT
GGATTCTGCG
CGGGACGTCC
TTCTGCTACG
TCCCTTCGGC
CCTCAATCCA
GCGGACCTTC
CTTCCCGCGG
CCTGCTGCCG
GCTCTGCGGC
CTCTTCCGCG
TCTTCGCCTT
CGCCCTCAGA
CGAGTCGGAT
CTCCCTTTGG
GCCGCCTCCC
CGCATCGATA
CCGTCGACCT
CGAGACCTAG
AAAAACATGG
AGCAATCACA
AGTAGCAATA
CAGCAGCTAC
CAATGCTGAT
TGTGCCTGGC
TAGAAGCACA
AGAGGAGGAG
GAGGTGGGTT
TTCCAGTCAC
ACCTCAGGTA
CCTTTAAGAC
CAATGACTTA
CAAGGCAGCT
GTAGATCTTA
GCCACTTTTT
AAAAGAAAAG
GGGGGACTGG
AAGGGCTAAT
TCACTCCCAA
CGAAGACAAG
ATATCCTTGA
TCTGTGGATC
TACCACACAC
AAGGCTACTT
CCCTGATTGG
CAGAACTACA
CACCAGGGCC
AGGGATCAGA
TATCCACTGA
CCTTTGGATG
GTGCTACAAG
CTAGTACCAG
TTGAGCAAGA
GAAGGTAGAA
GAAGCCAATG
AAGGAGAGAA
CACCCGCTTG
TTACACCCTG
TGAGCCTGCA
TGGGATGGAT
GACCCGGAGA
GAGAAGTATT
AGAGTGGAGG
TTTGACAGCC
GCCTAGCATT
TCATCACATG
GCCCGAGAGC
TGCATCCGGA
CTGTACTGGG
TCTCTCTGGT
TAGACCAGAT
CTGAGCCTGG
GAGCTCTCTG
GCTAACTAGG
GAACCCACTG
CTTAAGCCTC
AATAAAGCTT
GCCTTGAGTG
CTTCAAGTAG
TGTGTGCCCG
TCTGTTGTGT
GACTCTGGTA
ACTAGAGATC
CCTCAGACCC
TTTTAGTCAG
TGTGGAAAAT
CTCTAGCAGG
GCCCGTTTAA
ACCCGCTGAT
CAGCCTCGAC
TGTGCCTTCT
AGTTGCCAGC
CATCTGTTGT
TTGCCCCTCC
CCCGTGCCTT
CCTTGACCCT
GGAAGGTGCC
ACTCCCACTG
TCCTTTCCTA
ATAAAATGAG
GAAATTGCAT
CGCATTGTCT
GAGTAGGTGT
CATTCTATTC
TGGGGGGTGG
GGTGGGGCAG
GACAGCAAGG
GGGAGGATTG
GGAAGACAAT
AGCAGGCATG
CTGGGGATGC
GGTGGGCTCT
ATGGCTTCTG
AGGCGGAAAG
AACCAGCTGG
GGCTCTAGGG
GGTATCCCCA
CGCGCCCTGT
AGCGGCGCAT
TAAGCGCGGC
GGGTGTGGTG
GTTACGCGCA
GCGTGACCGC
TACACTTGCC
AGCGCCCTAG
CGCCCGCTCC
TTTCGCTTTC
TTCCCTTCCT
TTCTCGCCAC
GTTCGCCGGC
TTTCCCCGTC
AAGCTCTAAA
TCGGGGGCTC
CCTTTAGGGT
TCCGATTTAG
TGCTTTACGG
CACCTCGACC
CCAAAAAACT
TGATTAGGGT
GATGGTTCAC
GTAGTGGGCC
ATCGCCCTGA
TAGACGGTTT
TTCGCCCTTT
GACGTTGGAG
TCCACGTTCT
TTAATAGTGG
ACTCTTGTTC
CAAACTG