产品货号：P21236
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pLenti-Puro-sgAMPKa1 |
|---|---|
| 别称: | Plasmid #138684 |
| 基因长: | |
| 质粒宿主： | 哺乳细胞,慢病毒 |
| 质粒用途： | 基因编辑 |
| 片段类型： | CRISPR |
| 片段物种： | 人 |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | |
| 启动子: | hU6 |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | |
| 正向引物: | |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTCTA
TATAAGCAGC
GCGTTTTGCC
TGTACTGGGT
CTCTCTGGTT
AGACCAGATC
TGAGCCTGGG
AGCTCTCTGG
CTAACTAGGG
AACCCACTGC
TTAAGCCTCA
ATAAAGCTTG
CCTTGAGTGC
TTCAAGTAGT
GTGTGCCCGT
CTGTTGTGTG
ACTCTGGTAA
CTAGAGATCC
CTCAGACCCT
TTTAGTCAGT
GTGGAAAATC
TCTAGCAGTG
GCGCCCGAAC
AGGGACTTGA
AAGCGAAAGG
GAAACCAGAG
GAGCTCTCTC
GACGCAGGAC
TCGGCTTGCT
GAAGCGCGCA
CGGCAAGAGG
CGAGGGGCGG
CGACTGGTGA
GTACGCCAAA
AATTTTGACT
AGCGGAGGCT
AGAAGGAGAG
AGATGGGTGC
GAGAGCGTCA
GTATTAAGCG
GGGGAGAATT
AGATCGCGAT
GGGAAAAAAT
TCGGTTAAGG
CCAGGGGGAA
AGAAAAAATA
TAAATTAAAA
CATATAGTAT
GGGCAAGCAG
GGAGCTAGAA
CGATTCGCAG
TTAATCCTGG
CCTGTTAGAA
ACATCAGAAG
GCTGTAGACA
AATACTGGGA
CAGCTACAAC
CATCCCTTCA
GACAGGATCA
GAAGAACTTA
GATCATTATA
TAATACAGTA
GCAACCCTCT
ATTGTGTGCA
TCAAAGGATA
GAGATAAAAG
ACACCAAGGA
AGCTTTAGAC
AAGATAGAGG
AAGAGCAAAA
CAAAAGTAAG
ACCACCGCAC
AGCAAGCGGC
CGCTGATCTT
CAGACCTGGA
GGAGGAGATA
TGAGGGACAA
TTGGAGAAGT
GAATTATATA
AATATAAAGT
AGTAAAAATT
GAACCATTAG
GAGTAGCACC
CACCAAGGCA
AAGAGAAGAG
TGGTGCAGAG
AGAAAAAAGA
GCAGTGGGAA
TAGGAGCTTT
GTTCCTTGGG
TTCTTGGGAG
CAGCAGGAAG
CACTATGGGC
GCAGCGTCAA
TGACGCTGAC
GGTACAGGCC
AGACAATTAT
TGTCTGGTAT
AGTGCAGCAG
CAGAACAATT
TGCTGAGGGC
TATTGAGGCG
CAACAGCATC
TGTTGCAACT
CACAGTCTGG
GGCATCAAGC
AGCTCCAGGC
AAGAATCCTG
GCTGTGGAAA
GATACCTAAA
GGATCAACAG
CTCCTGGGGA
TTTGGGGTTG
CTCTGGAAAA
CTCATTTGCA
CCACTGCTGT
GCCTTGGAAT
GCTAGTTGGA
GTAATAAATC
TCTGGAACAG
ATTTGGAATC
ACACGACCTG
GATGGAGTGG
GACAGAGAAA
TTAACAATTA
CACAAGCTTA
ATACACTCCT
TAATTGAAGA
ATCGCAAAAC
CAGCAAGAAA
AGAATGAACA
AGAATTATTG
GAATTAGATA
AATGGGCAAG
TTTGTGGAAT
TGGTTTAACA
TAACAAATTG
GCTGTGGTAT
ATAAAATTAT
TCATAATGAT
AGTAGGAGGC
TTGGTAGGTT
TAAGAATAGT
TTTTGCTGTA
CTTTCTATAG
TGAATAGAGT
TAGGCAGGGA
TATTCACCAT
TATCGTTTCA
GACCCACCTC
CCAACCCCGA
GGGGACCCGA
CAGGCCCGAA
GGAATAGAAG
AAGAAGGTGG
AGAGAGAGAC
AGAGACAGAT
CCATTCGATT
AGTGAACGGA
TCGGCACTGC
GTGCGCCAAT
TCTGCAGACA
AATGGCAGTA
TTCATCCACA
ATTTTAAAAG
AAAAGGGGGG
ATTGGGGGGT
ACAGTGCAGG
GGAAAGAATA
GTAGACATAA
TAGCAACAGA
CATACAAACT
AAAGAATTAC
AAAAACAAAT
TACAAAAATT
CAAAATTTTC
GGGTTTATTA
CAGGGACAGC
AGAGATCCAG
TTTGGTTAAT
TAAGGTACCG
AGGGCCTATT
TCCCATGATT
CCTTCATATT
TGCATATACG
ATACAAGGCT
GTTAGAGAGA
TAATTAGAAT
TAATTTGACT
GTAAACACAA
AGATATTAGT
ACAAAATACG
TGACGTAGAA
AGTAATAATT
TCTTGGGTAG
TTTGCAGTTT
TAAAATTATG
TTTTAAAATG
GACTATCATA
TGCTTACCGT
AACTTGAAAG
TATTTCGATT
TCTTGGCTTT
ATATATCTTG
TGGAAAGGAC
GAAACACCGC
TGGTGTGGAT
TATTGTCACG
TTTTAGAGCT
AGAAATAGCA
AGTTAAAATA
AGGCTAGTCC
GTTATCAACT
TGAAAAAGTG
GCACCGAGTC
GGTGCTTTTT
TGAATTCGCT
AGCTAGGTCT
TGAAAGGAGT
GGGAATTGGC
TCCGGTGCCC
GTCAGTGGGC
AGAGCGCACA
TCGCCCACAG
TCCCCGAGAA
GTTGGGGGGA
GGGGTCGGCA
ATTGATCCGG
TGCCTAGAGA
AGGTGGCGCG
GGGTAAACTG
GGAAAGTGAT
GTCGTGTACT
GGCTCCGCCT
TTTTCCCGAG
GGTGGGGGAG
AACCGTATAT
AAGTGCAGTA
GTCGCCGTGA
ACGTTCTTTT
TCGCAACGGG
TTTGCCGCCA
GAACACAGGA
CCGGTTCTAG
AGCGCTGCCA
CCATGGACAA
GAAGTACAGC
ATCGGCCTGG
ACATCGGCAC
CAACTCTGTG
GGCTGGGCCG
TGATCACCGA
CGAGTACAAG
GTGCCCAGCA
AGAAATTCAA
GGTGCTGGGC
AACACCGACC
GGCACAGCAT
CAAGAAGAAC
CTGATCGGAG
CCCTGCTGTT
CGACAGCGGC
GAAACAGCCG
AGGCCACCCG
GCTGAAGAGA
ACCGCCAGAA
GAAGATACAC
CAGACGGAAG
AACCGGATCT
GCTATCTGCA
AGAGATCTTC
AGCAACGAGA
TGGCCAAGGT
GGACGACAGC
TTCTTCCACA
GACTGGAAGA
GTCCTTCCTG
GTGGAAGAGG
ATAAGAAGCA
CGAGCGGCAC
CCCATCTTCG
GCAACATCGT
GGACGAGGTG
GCCTACCACG
AGAAGTACCC
CACCATCTAC
CACCTGAGAA
AGAAACTGGT
GGACAGCACC
GACAAGGCCG
ACCTGCGGCT
GATCTATCTG
GCCCTGGCCC
ACATGATCAA
GTTCCGGGGC
CACTTCCTGA
TCGAGGGCGA
CCTGAACCCC
GACAACAGCG
ACGTGGACAA
GCTGTTCATC
CAGCTGGTGC
AGACCTACAA
CCAGCTGTTC
GAGGAAAACC
CCATCAACGC
CAGCGGCGTG
GACGCCAAGG
CCATCCTGTC
TGCCAGACTG
AGCAAGAGCA
GACGGCTGGA
AAATCTGATC
GCCCAGCTGC
CCGGCGAGAA
GAAGAATGGC
CTGTTCGGAA
ACCTGATTGC
CCTGAGCCTG
GGCCTGACCC
CCAACTTCAA
GAGCAACTTC
GACCTGGCCG
AGGATGCCAA
ACTGCAGCTG
AGCAAGGACA
CCTACGACGA
CGACCTGGAC
AACCTGCTGG
CCCAGATCGG
CGACCAGTAC
GCCGACCTGT
TTCTGGCCGC
CAAGAACCTG
TCCGACGCCA
TCCTGCTGAG
CGACATCCTG
AGAGTGAACA
CCGAGATCAC
CAAGGCCCCC
CTGAGCGCCT
CTATGATCAA
GAGATACGAC
GAGCACCACC
AGGACCTGAC
CCTGCTGAAA
GCTCTCGTGC
GGCAGCAGCT
GCCTGAGAAG
TACAAAGAGA
TTTTCTTCGA
CCAGAGCAAG
AACGGCTACG
CCGGCTACAT
TGACGGCGGA
GCCAGCCAGG
AAGAGTTCTA
CAAGTTCATC
AAGCCCATCC
TGGAAAAGAT
GGACGGCACC
GAGGAACTGC
TCGTGAAGCT
GAACAGAGAG
GACCTGCTGC
GGAAGCAGCG
GACCTTCGAC
AACGGCAGCA
TCCCCCACCA
GATCCACCTG
GGAGAGCTGC
ACGCCATTCT
GCGGCGGCAG
GAAGATTTTT
ACCCATTCCT
GAAGGACAAC
CGGGAAAAGA
TCGAGAAGAT
CCTGACCTTC
CGCATCCCCT
ACTACGTGGG
CCCTCTGGCC
AGGGGAAACA
GCAGATTCGC
CTGGATGACC
AGAAAGAGCG
AGGAAACCAT
CACCCCCTGG
AACTTCGAGG
AAGTGGTGGA
CAAGGGCGCT
TCCGCCCAGA
GCTTCATCGA
GCGGATGACC
AACTTCGATA
AGAACCTGCC
CAACGAGAAG
GTGCTGCCCA
AGCACAGCCT
GCTGTACGAG
TACTTCACCG
TGTATAACGA
GCTGACCAAA
GTGAAATACG
TGACCGAGGG
AATGAGAAAG
CCCGCCTTCC
TGAGCGGCGA
GCAGAAAAAG
GCCATCGTGG
ACCTGCTGTT
CAAGACCAAC
CGGAAAGTGA
CCGTGAAGCA
GCTGAAAGAG
GACTACTTCA
AGAAAATCGA
GTGCTTCGAC
TCCGTGGAAA
TCTCCGGCGT
GGAAGATCGG
TTCAACGCCT
CCCTGGGCAC
ATACCACGAT
CTGCTGAAAA
TTATCAAGGA
CAAGGACTTC
CTGGACAATG
AGGAAAACGA
GGACATTCTG
GAAGATATCG
TGCTGACCCT
GACACTGTTT
GAGGACAGAG
AGATGATCGA
GGAACGGCTG
AAAACCTATG
CCCACCTGTT
CGACGACAAA
GTGATGAAGC
AGCTGAAGCG
GCGGAGATAC
ACCGGCTGGG
GCAGGCTGAG
CCGGAAGCTG
ATCAACGGCA
TCCGGGACAA
GCAGTCCGGC
AAGACAATCC
TGGATTTCCT
GAAGTCCGAC
GGCTTCGCCA
ACAGAAACTT
CATGCAGCTG
ATCCACGACG
ACAGCCTGAC
CTTTAAAGAG
GACATCCAGA
AAGCCCAGGT
GTCCGGCCAG
GGCGATAGCC
TGCACGAGCA
CATTGCCAAT
CTGGCCGGCA
GCCCCGCCAT
TAAGAAGGGC
ATCCTGCAGA
CAGTGAAGGT
GGTGGACGAG
CTCGTGAAAG
TGATGGGCCG
GCACAAGCCC
GAGAACATCG
TGATCGAAAT
GGCCAGAGAG
AACCAGACCA
CCCAGAAGGG
ACAGAAGAAC
AGCCGCGAGA
GAATGAAGCG
GATCGAAGAG
GGCATCAAAG
AGCTGGGCAG
CCAGATCCTG
AAAGAACACC
CCGTGGAAAA
CACCCAGCTG
CAGAACGAGA
AGCTGTACCT
GTACTACCTG
CAGAATGGGC
GGGATATGTA
CGTGGACCAG
GAACTGGACA
TCAACCGGCT
GTCCGACTAC
GATGTGGACC
ATATCGTGCC
TCAGAGCTTT
CTGAAGGACG
ACTCCATCGA
CAACAAGGTG
CTGACCAGAA
GCGACAAGAA
CCGGGGCAAG
AGCGACAACG
TGCCCTCCGA
AGAGGTCGTG
AAGAAGATGA
AGAACTACTG
GCGGCAGCTG
CTGAACGCCA
AGCTGATTAC
CCAGAGAAAG
TTCGACAATC
TGACCAAGGC
CGAGAGAGGC
GGCCTGAGCG
AACTGGATAA
GGCCGGCTTC
ATCAAGAGAC
AGCTGGTGGA
AACCCGGCAG
ATCACAAAGC
ACGTGGCACA
GATCCTGGAC
TCCCGGATGA
ACACTAAGTA
CGACGAGAAT
GACAAGCTGA
TCCGGGAAGT
GAAAGTGATC
ACCCTGAAGT
CCAAGCTGGT
GTCCGATTTC
CGGAAGGATT
TCCAGTTTTA
CAAAGTGCGC
GAGATCAACA
ACTACCACCA
CGCCCACGAC
GCCTACCTGA
ACGCCGTCGT
GGGAACCGCC
CTGATCAAAA
AGTACCCTAA
GCTGGAAAGC
GAGTTCGTGT
ACGGCGACTA
CAAGGTGTAC
GACGTGCGGA
AGATGATCGC
CAAGAGCGAG
CAGGAAATCG
GCAAGGCTAC
CGCCAAGTAC
TTCTTCTACA
GCAACATCAT
GAACTTTTTC
AAGACCGAGA
TTACCCTGGC
CAACGGCGAG
ATCCGGAAGC
GGCCTCTGAT
CGAGACAAAC
GGCGAAACCG
GGGAGATCGT
GTGGGATAAG
GGCCGGGATT
TTGCCACCGT
GCGGAAAGTG
CTGAGCATGC
CCCAAGTGAA
TATCGTGAAA
AAGACCGAGG
TGCAGACAGG
CGGCTTCAGC
AAAGAGTCTA
TCCTGCCCAA
GAGGAACAGC
GATAAGCTGA
TCGCCAGAAA
GAAGGACTGG
GACCCTAAGA
AGTACGGCGG
CTTCGACAGC
CCCACCGTGG
CCTATTCTGT
GCTGGTGGTG
GCCAAAGTGG
AAAAGGGCAA
GTCCAAGAAA
CTGAAGAGTG
TGAAAGAGCT
GCTGGGGATC
ACCATCATGG
AAAGAAGCAG
CTTCGAGAAG
AATCCCATCG
ACTTTCTGGA
AGCCAAGGGC
TACAAAGAAG
TGAAAAAGGA
CCTGATCATC
AAGCTGCCTA
AGTACTCCCT
GTTCGAGCTG
GAAAACGGCC
GGAAGAGAAT
GCTGGCCTCT
GCCGGCGAAC
TGCAGAAGGG
AAACGAACTG
GCCCTGCCCT
CCAAATATGT
GAACTTCCTG
TACCTGGCCA
GCCACTATGA
GAAGCTGAAG
GGCTCCCCCG
AGGATAATGA
GCAGAAACAG
CTGTTTGTGG
AACAGCACAA
GCACTACCTG
GACGAGATCA
TCGAGCAGAT
CAGCGAGTTC
TCCAAGAGAG
TGATCCTGGC
CGACGCTAAT
CTGGACAAAG
TGCTGTCCGC
CTACAACAAG
CACCGGGATA
AGCCCATCAG
AGAGCAGGCC
GAGAATATCA
TCCACCTGTT
TACCCTGACC
AATCTGGGAG
CCCCTGCCGC
CTTCAAGTAC
TTTGACACCA
CCATCGACCG
GAAGAGGTAC
ACCAGCACCA
AAGAGGTGCT
GGACGCCACC
CTGATCCACC
AGAGCATCAC
CGGCCTGTAC
GAGACACGGA
TCGACCTGTC
TCAGCTGGGA
GGCGACAAGC
GACCTGCCGC
CACAAAGAAG
GCTGGACAGG
CTAAGAAGAA
GAAAGATTAC
AAAGACGATG
ACGATAAGGG
ATCCGGCGCA
ACAAACTTCT
CTCTGCTGAA
ACAAGCCGGA
GATGTCGAAG
AGAATCCTGG
ACCGACCGAG
TACAAGCCCA
CGGTGCGCCT
CGCCACCCGC
GACGACGTCC
CCAGGGCCGT
ACGCACCCTC
GCCGCCGCGT
TCGCCGACTA
CCCCGCCACG
CGCCACACCG
TCGATCCGGA
CCGCCACATC
GAGCGGGTCA
CCGAGCTGCA
AGAACTCTTC
CTCACGCGCG
TCGGGCTCGA
CATCGGCAAG
GTGTGGGTCG
CGGACGACGG
CGCCGCGGTG
GCGGTCTGGA
CCACGCCGGA
GAGCGTCGAA
GCGGGGGCGG
TGTTCGCCGA
GATCGGCCCG
CGCATGGCCG
AGTTGAGCGG
TTCCCGGCTG
GCCGCGCAGC
AACAGATGGA
AGGCCTCCTG
GCGCCGCACC
GGCCCAAGGA
GCCCGCGTGG
TTCCTGGCCA
CCGTCGGAGT
CTCGCCCGAC
CACCAGGGCA
AGGGTCTGGG
CAGCGCCGTC
GTGCTCCCCG
GAGTGGAGGC
GGCCGAGCGC
GCCGGGGTGC
CCGCCTTCCT
GGAGACCTCC
GCGCCCCGCA
ACCTCCCCTT
CTACGAGCGG
CTCGGCTTCA
CCGTCACCGC
CGACGTCGAG
GTGCCCGAAG
GACCGCGCAC
CTGGTGCATG
ACCCGCAAGC
CCGGTGCCTG
AACGCGTTAA
GTCGACAATC
AACCTCTGGA
TTACAAAATT
TGTGAAAGAT
TGACTGGTAT
TCTTAACTAT
GTTGCTCCTT
TTACGCTATG
TGGATACGCT
GCTTTAATGC
CTTTGTATCA
TGCTATTGCT
TCCCGTATGG
CTTTCATTTT
CTCCTCCTTG
TATAAATCCT
GGTTGCTGTC
TCTTTATGAG
GAGTTGTGGC
CCGTTGTCAG
GCAACGTGGC
GTGGTGTGCA
CTGTGTTTGC
TGACGCAACC
CCCACTGGTT
GGGGCATTGC
CACCACCTGT
CAGCTCCTTT
CCGGGACTTT
CGCTTTCCCC
CTCCCTATTG
CCACGGCGGA
ACTCATCGCC
GCCTGCCTTG
CCCGCTGCTG
GACAGGGGCT
CGGCTGTTGG
GCACTGACAA
TTCCGTGGTG
TTGTCGGGGA
AATCATCGTC
CTTTCCTTGG
CTGCTCGCCT
GTGTTGCCAC
CTGGATTCTG
CGCGGGACGT
CCTTCTGCTA
CGTCCCTTCG
GCCCTCAATC
CAGCGGACCT
TCCTTCCCGC
GGCCTGCTGC
CGGCTCTGCG
GCCTCTTCCG
CGTCTTCGCC
TTCGCCCTCA
GACGAGTCGG
ATCTCCCTTT
GGGCCGCCTC
CCCGCGTCGA
CTTTAAGACC
AATGACTTAC
AAGGCAGCTG
TAGATCTTAG
CCACTTTTTA
AAAGAAAAGG
GGGGACTGGA
AGGGCTAATT
CACTCCCAAC
GAAGACAAGA
TCTGCTTTTT
GCTTGTACTG
GGTCTCTCTG
GTTAGACCAG
ATCTGAGCCT
GGGAGCTCTC
TGGCTAACTA
GGGAACCCAC
TGCTTAAGCC
TCAATAAAGC
TTGCCTTGAG
TGCTTCAAGT
AGTGTGTGCC
CGTCTGTTGT
GTGACTCTGG
TAACTAGAGA
TCCCTCAGAC
CCTTTTAGTC
AGTGTGGAAA
ATCTCTAGCA
GGGCCCGTTT
AAACCCGCTG
ATCAGCCTCG
ACTGTGCCTT
CTAGTTGCCA
GCCATCTGTT
GTTTGCCCCT
CCCCCGTGCC
TTCCTTGACC
CTGGAAGGTG
CCACTCCCAC
TGTCCTTTCC
TAATAAAATG
AGGAAATTGC
ATCGCATTGT
CTGAGTAGGT
GTCATTCTAT
TCTGGGGGGT
GGGGTGGGGC
AGGACAGCAA
GGGGGAGGAT
TGGGAAGACA
ATAGCAGGCA
TGCTGGGGAT
GCGGTGGGCT
CTATGGCTTC
TGAGGCGGAA
AGAACCAGCT
GGGGCTCTAG
GGGGTATCCC
CACGCGCCCT
GTAGCGGCGC
ATTAAGCGCG
GCGGGTGTGG
TGGTTACGCG
CAGCGTGACC
GCTACACTTG
CCAGCGCCCT
AGCGCCCGCT
CCTTTCGCTT
TCTTCCCTTC
CTTTCTCGCC
ACGTTCGCCG
GCTTTCCCCG
TCAAGCTCTA
AATCGGGGGC
TCCCTTTAGG
GTTCCGATTT
AGTGCTTTAC
GGCACCTCGA
CCCCAAAAAA
CTTGATTAGG
GTGATGGTTC
ACGTAGTGGG
CCATCGCCCT
GATAGACGGT
TTTTCGCCCT
TTGACGTTGG
AGTCCACGTT
CTTTAATAGT
GGACTCTTGT
TCCAAACTGG
AACAACACTC
AACCCTATCT
CGGTCTATTC
TTTTGATTTA
TAAGGGATTT
TGCCGATTTC
GGCCTATTGG
TTAAAAAATG
AGCTGATTTA
ACAAAAATTT
AACGCGAATT
AATTCTGTGG
AATGTGTGTC
AGTTAGGGTG
TGGAAAGTCC
CCAGGCTCCC
CAGCAGGCAG
AAGTATGCAA
AGCATGCATC
TCAATTAGTC
AGCAACCAGG
TGTGGAAAGT
CCCCAGGCTC
CCCAGCAGGC
AGAAGTATGC
AAAGCATGCA
TCTCAATTAG
TCAGCAACCA
TAGTCCCGCC
CCTAACTCCG
CCCATCCCGC
CCCTAACTCC
GCCCAGTTCC
GCCCATTCTC
CGCCCCATGG
CTGACTAATT
TTTTTTATTT
ATGCAGAGGC
CGAGGCCGCC
TCTGCCTCTG
AGCTATTCCA
GAAGTAGTGA
GGAGGCTTTT
TTGGAGGCCT
AGGCTTTTGC
AAAAAGCTCC
CGGGAGCTTG
TATATCCATT
TTCGGATCTG
ATCAGCACGT
GTTGACAATT
AATCATCGGC
ATAGTATATC
GGCATAGTAT
AATACGACAA
GGTGAGGAAC
TAAACCATGG
CCAAGTTGAC
CAGTGCCGTT
CCGGTGCTCA
CCGCGCGCGA
CGTCGCCGGA
GCGGTCGAGT
TCTGGACCGA
CCGGCTCGGG
TTCTCCCGGG
ACTTCGTGGA
GGACGACTTC
GCCGGTGTGG
TCCGGGACGA
CGTGACCCTG
TTCATCAGCG
CGGTCCAGGA
CCAGGTGGTG
CCGGACAACA
CCCTGGCCTG
GGTGTGGGTG
CGCGGCCTGG
ACGAGCTGTA
CGCCGAGTGG
TCGGAGGTCG
TGTCCACGAA
CTTCCGGGAC
GCCTCCGGGC
CGGCCATGAC
CGAGATCGGC
GAGCAGCCGT
GGGGGCGGGA
GTTCGCCCTG
CGCGACCCGG
CCGGCAACTG
CGTGCACTTC
GTGGCCGAGG
AGCAGGACTG
ACACGTGCTA
CGAGATTTCG
ATTCCACCGC
CGCCTTCTAT
GAAAGGTTGG
GCTTCGGAAT
CGTTTTCCGG
GACGCCGGCT
GGATGATCCT
CCAGCGCGGG
GATCTCATGC
TGGAGTTCTT
CGCCCACCCC
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GTATACCGTC
GACCTCTAGC
TAGAGCTTGG
CGTAATCATG
GTCATAGCTG
TTTCCTGTGT
GAAATTGTTA
TCCGCTCACA
ATTCCACACA
ACATACGAGC
CGGAAGCATA
AAGTGTAAAG
CCTGGGGTGC
CTAATGAGTG
AGCTAACTCA
CATTAATTGC
GTTGCGCTCA
CTGCCCGCTT
TCCAGTCGGG
AAACCTGTCG
TGCCAGCTGC
ATTAATGAAT
CGGCCAACGC
GCGGGGAGAG
GCGGTTTGCG
TATTGGGCGC
TCTTCCGCTT
CCTCGCTCAC
TGACTCGCTG
CGCTCGGTCG
TTCGGCTGCG
GCGAGCGGTA
TCAGCTCACT
CAAAGGCGGT
AATACGGTTA
TCCACAGAAT
CAGGGGATAA
CGCAGGAAAG
AACATGTGAG
CAAAAGGCCA
GCAAAAGGCC
AGGAACCGTA
AAAAGGCCGC
GTTGCTGGCG
TTTTTCCATA
GGCTCCGCCC
CCCTGACGAG
CATCACAAAA
ATCGACGCTC
AAGTCAGAGG
TGGCGAAACC
CGACAGGACT
ATAAAGATAC
CAGGCGTTTC
CCCCTGGAAG
CTCCCTCGTG
CGCTCTCCTG
TTCCGACCCT
GCCGCTTACC
GGATACCTGT
CCGCCTTTCT
CCCTTCGGGA
AGCGTGGCGC
TTTCTCATAG
CTCACGCTGT
AGGTATCTCA
GTTCGGTGTA
GGTCGTTCGC
TCCAAGCTGG
GCTGTGTGCA
CGAACCCCCC
GTTCAGCCCG
ACCGCTGCGC
CTTATCCGGT
AACTATCGTC
TTGAGTCCAA
CCCGGTAAGA
CACGACTTAT
CGCCACTGGC
AGCAGCCACT
GGTAACAGGA
TTAGCAGAGC
GAGGTATGTA
GGCGGTGCTA
CAGAGTTCTT
GAAGTGGTGG
CCTAACTACG
GCTACACTAG
AAGAACAGTA
TTTGGTATCT
GCGCTCTGCT
GAAGCCAGTT
ACCTTCGGAA
AAAGAGTTGG
TAGCTCTTGA
TCCGGCAAAC
AAACCACCGC
TGGTAGCGGT
GGTTTTTTTG
TTTGCAAGCA
GCAGATTACG
CGCAGAAAAA
AAGGATCTCA
AGAAGATCCT
TTGATCTTTT
CTACGGGGTC
TGACGCTCAG
TGGAACGAAA
ACTCACGTTA
AGGGATTTTG
GTCATGAGAT
TATCAAAAAG
GATCTTCACC
TAGATCCTTT
TAAATTAAAA
ATGAAGTTTT
AAATCAATCT
AAAGTATATA
TGAGTAAACT
TGGTCTGACA
GTTACCAATG
CTTAATCAGT
GAGGCACCTA
TCTCAGCGAT
CTGTCTATTT
CGTTCATCCA
TAGTTGCCTG
ACTCCCCGTC
GTGTAGATAA
CTACGATACG
GGAGGGCTTA
CCATCTGGCC
CCAGTGCTGC
AATGATACCG
CGAGACCCAC
GCTCACCGGC
TCCAGATTTA
TCAGCAATAA
ACCAGCCAGC
CGGAAGGGCC
GAGCGCAGAA
GTGGTCCTGC
AACTTTATCC
GCCTCCATCC
AGTCTATTAA
TTGTTGCCGG
GAAGCTAGAG
TAAGTAGTTC
GCCAGTTAAT
AGTTTGCGCA
ACGTTGTTGC
CATTGCTACA
GGCATCGTGG
TGTCACGCTC
GTCGTTTGGT
ATGGCTTCAT
TCAGCTCCGG
TTCCCAACGA
TCAAGGCGAG
TTACATGATC
CCCCATGTTG
TGCAAAAAAG
CGGTTAGCTC
CTTCGGTCCT
CCGATCGTTG
TCAGAAGTAA
GTTGGCCGCA
GTGTTATCAC
TCATGGTTAT
GGCAGCACTG
CATAATTCTC
TTACTGTCAT
GCCATCCGTA
AGATGCTTTT
CTGTGACTGG
TGAGTACTCA
ACCAAGTCAT
TCTGAGAATA
GTGTATGCGG
CGACCGAGTT
GCTCTTGCCC
GGCGTCAATA
CGGGATAATA
CCGCGCCACA
TAGCAGAACT
TTAAAAGTGC
TCATCATTGG
AAAACGTTCT
TCGGGGCGAA
AACTCTCAAG
GATCTTACCG
CTGTTGAGAT
CCAGTTCGAT
GTAACCCACT
CGTGCACCCA
ACTGATCTTC
AGCATCTTTT
ACTTTCACCA
GCGTTTCTGG
GTGAGCAAAA
ACAGGAAGGC
AAAATGCCGC
AAAAAAGGGA
ATAAGGGCGA
CACGGAAATG
TTGAATACTC
ATACTCTTCC
TTTTTCAATA
TTATTGAAGC
ATTTATCAGG
GTTATTGTCT
CATGAGCGGA
TACATATTTG
AATGTATTTA
GAAAAATAAA
CAAATAGGGG
TTCCGCGCAC
ATTTCCCCGA
AAAGTGCCAC
CTGACGTCGA
CGGATCGGGA
GATCTCCCGA
TCCCCTATGG
TGCACTCTCA
GTACAATCTG
CTCTGATGCC
GCATAGTTAA
GCCAGTATCT
GCTCCCTGCT
TGTGTGTTGG
AGGTCGCTGA
GTAGTGCGCG
AGCAAAATTT
AAGCTACAAC
AAGGCAAGGC
TTGACCGACA
ATTGCATGAA
GAATCTGCTT
AGGGTTAGGC
GTTTTGCGCT
GCTTCGCGAT
GTACGGGCCA
GATATACGCG
TTGACATTGA
TTATTGACTA
GTTATTAATA
GTAATCAATT
ACGGGGTCAT
TAGTTCATAG
CCCATATATG
GAGTTCCGCG
TTACATAACT
TACGGTAAAT
GGCCCGCCTG
GCTGACCGCC
CAACGACCCC
CGCCCATTGA
CGTCAATAAT
GACGTATGTT
CCCATAGTAA
CGCCAATAGG
GACTTTCCAT
TGACGTCAAT
GGGTGGAGTA
TTTACGGTAA
ACTGCCCACT
TGGCAGTACA
TCAAGTGTAT
CATATGCCAA
GTACGCCCCC
TATTGACGTC
AATGACGGTA
AATGGCCCGC
CTGGCATTAT
GCCCAGTACA
TGACCTTATG
GGACTTTCCT
ACTTGGCAGT
ACATCTACGT
ATTAGTCATC
GCTATTACCA
TGG
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