名称: | pHT43-SUMO-GFP |
---|---|
别称: | |
基因长: | |
质粒宿主: | 枯草杆菌 |
质粒用途: | 蛋白表达,分泌表达 |
片段类型: | ORF |
片段物种: | |
原核抗性: | Amp |
筛选标记: | Chl |
荧光标记: | 绿色 |
启动子: | Pgrac |
复制子: | pUC,repB |
拷贝数: | |
感受态: | Stbl3 |
温度: | 30度 |
背景载体: | |
正向引物: | M13F:TGTAAAACGACGGCCAGT |
反向引物: | pHT-R:agcaagtcatctccaaattctt |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TTAAGTTATT
GGTATGACTG
GTTTTAAGCG
CAAAAAAAGT
TGCTTTTTCG
TACCTATTAA
TGTATCGTTT
TAGAAAACCG
ACTGTAAAAA
GTACAGTCGG
CATTATCTCA
TATTATAAAA
GCCAGTCATT
AGGCCTATCT
GACAATTCCT
GAATAGAGTT
CATAAACAAT
CCTGCATGAT
AACCATCACA
AACAGAATGA
TGTACCTGTA
AAGATAGCGG
TAAATATATT
GAATTACCTT
TATTAATGAA
TTTTCCTGCT
GTAATAATGG
GTAGAAGGTA
ATTACTATTA
TTATTGATAT
TTAAGTTAAA
CCCAGTAAAT
GAAGTCCATG
GAATAATAGA
AAGAGAAAAA
GCATTTTCAG
GTATAGGTGT
TTTGGGAAAC
AATTTCCCCG
AACCATTATA
TTTCTCTACA
TCAGAAAGGT
ATAAATCATA
AAACTCTTTG
AAGTCATTCT
TTACAGGAGT
CCAAATACCA
GAGAATGTTT
TAGATACACC
ATCAAAAATT
GTATAAAGTG
GCTCTAACTT
ATCCCAATAA
CCTAACTCTC
CGTCGCTATT
GTAACCAGTT
CTAAAAGCTG
TATTTGAGTT
TATCACCCTT
GTCACTAAGA
AAATAAATGC
AGGGTAAAAT
TTATATCCTT
CTTGTTTTAT
GTTTCGGTAT
AAAACACTAA
TATCAATTTC
TGTGGTTATA
CTAAAAGTCG
TTTGTTGGTT
CAAATAATGA
TTAAATATCT
CTTTTCTCTT
CCAATTGTCT
AAATCAATTT
TATTAAAGTT
CATTTGATAT
GCCTCCTAAA
TTTTTATCTA
AAGTGAATTT
AGGAGGCTTA
CTTGTCTGCT
TTCTTCATTA
GAATCAATCC
TTTTTTAAAA
GTCAATATTA
CTGTAACATA
AATATATATT
TTAAAAATAT
CCCACTTTAT
CCAATTTTCG
TTTGTTGAAC
TAATGGGTGC
TTTAGTTGAA
GAATAAAAGA
CCACATTAAA
AAATGTGGTC
TTTTGTGTTT
TTTTAAAGGA
TTTGAGCGTA
GCGAAAAATC
CTTTTCTTTC
TTATCTTGAT
AATAAGGGTA
ACTATTGCCG
TCGTCCATTC
CGACAGCATC
GCCAGTCACT
ATGGCGTGCT
GCTAGCGCCA
TTCGCCATTC
AGGCTGCGCA
ACTGTTGGGA
AGGGCGATCG
GTGCGGGCCT
CTTCGCTATT
ACGCCAGCTG
GCGAAAGGGG
GATGTGCTGC
AAGGCGATTA
AGTTGGGTAA
CGCCAGGGTT
TTCCCAGTCA
CGACGTTGTA
AAACGACGGC
CAGTGAATTC
GAGCTCAGGC
CTTAACTCAC
ATTAATTGCG
TTGCGCTCAC
TGCCCGCTTT
CCAGTCGGGA
AACCTGTCGT
GCCAGCTGCA
TTAATGAATC
GGCCAACGCG
CGGGGAGAGG
CGGTTTGCGT
ATTGGGCGCC
AGGGTGGTTT
TTCTTTTCAC
CAGTGAGACG
GGCAACAGCT
GATTGCCCTT
CACCGCCTGG
CCCTGAGAGA
GTTGCAGCAA
GCGGTCCACG
CTGGTTTGCC
CCAGCAGGCG
AAAATCCTGT
TTGATGGTGG
TTGACGGCGG
GATATAACAT
GAGCTGTCTT
CGGTATCGTC
GTATCCCACT
ACCGAGATAT
CCGCACCAAC
GCGCAGCCCG
GACTCGGTAA
TGGCGCGCAT
TGCGCCCAGC
GCCATCTGAT
CGTTGGCAAC
CAGCATCGCA
GTGGGAACGA
TGCCCTCATT
CAGCATTTGC
ATGGTTTGTT
GAAAACCGGA
CATGGCACTC
CAGTCGCCTT
CCCGTTCCGC
TATCGGCTGA
ATTTGATTGC
GAGTGAGATA
TTTATGCCAG
CCAGCCAGAC
GCAGACGCGC
CGAGACAGAA
CTTAATGGGC
CCGCTAACAG
CGCGATTTGC
TGGTGACCCA
ATGCGACCAG
ATGCTCCACG
CCCAGTCGCG
TACCGTCTTC
ATGGGAGAAA
ATAATACTGT
TGATGGGTGT
CTGGTCAGAG
ACATCAAGAA
ATAACGCCGG
AACATTAGTG
CAGGCAGCTT
CCACAGCAAT
GGCATCCTGG
TCATCCAGCG
GATAGTTAAT
GATCAGCCCA
CTGACGCGTT
GCGCGAGAAG
ATTGTGCACC
GCCGCTTTAC
AGGCTTCGAC
GCCGCTTCGT
TCTACCATCG
ACACCACCAC
GCTGGCACCC
AGTTGATCGG
CGCGAGATTT
AATCGCCGCG
ACAATTTGCG
ACGGCGCGTG
CAGGGCCAGA
CTGGAGGTGG
CAACGCCAAT
CAGCAACGAC
TGTTTGCCCG
CCAGTTGTTG
TGCCACGCGG
TTGGGAATGT
AATTCAGCTC
CGCCATCGCC
GCTTCCACTT
TTTCCCGCGT
TTTCGCAGAA
ACGTGGCTGG
CCTGGTTCAC
CACGCGGGAA
ACGGTCTGAT
AAGAGACACC
GGCATACTCT
GCGACATCGT
ATAACGTTAC
TGGTTTCATC
AAAATCGTCT
CCCTCCGTTT
GAATATTTGA
TTGATCGTAA
CCAGATGAAG
CACTCTTTCC
ACTATCCCTA
CAGTGTTATG
GCTTGAACAA
TCACGAAACA
ATAATTGGTA
CGTACGATCT
TTCAGCCGAC
TCAAACATCA
AATCTTACAA
ATGTAGTCTT
TGAAAGTATT
ACATATGTAA
GATTTAAATG
CAACCGTTTT
TTCGGAAGGA
AATGATGACC
TCGTTTCCAC
CGGAATTAGC
TTGGTACCAG
CTATTGTAAC
ATAATCGGTA
CGGGGGTGAA
AAAGCTAACG
GAAAAGGGAG
CGGAAAAGAA
TGATGTAAGC
GTGAAAAATT
TTTTATCTTA
TCACTTGAAA
TTGGAAGGGA
GATTCTTTAT
TATAAGAATT
GTGGAATTGT
GAGCGGATAA
CAATTCCCAA
TTAAAGGAGG
AAGGATCAAT
GATTCAAAAA
CGAAAGCGGA
CAGTTTCGTT
CAGACTTGTG
CTTATGTGCA
CGCTGTTATT
TGTCAGTTTG
CCGATTACAA
AAACATCAGC
CGTACTGGAT
CCGATGCATC
ATCATCATCA
TCATGGCTCT
CTTCAAGATT
CTGAAGTTAA
CCAAGAAGCT
AAACCTGAAG
TTAAACCTGA
AGTTAAACCT
GAAACACATA
TCAACCTTAA
AGTTTCTGAT
GGCTCTTCTG
AAATCTTCTT
CAAAATCAAA
AAAACAACAC
CTCTTCGTCG
TCTTATGGAA
GCTTTCGCTA
AACGTCAAGG
CAAAGAAATG
GATTCTCTTC
GTTTCCTTTA
CGATGGCATC
CGTATCCAAG
CTGATCAAGC
TCCTGAAGAT
CTTGATATGG
AAGATAACGA
TCATGAAGCT
CATCGTGAAC
AAATCGGCGG
CATGTCTAAA
GGCGAAGAAC
TTTTCACAGG
CGTTGTTCCT
ATCCTTGTTG
AACTTGATGG
CGATGTTAAC
GGCCATAAAT
TCTCTGTTTC
TGGCGAAGGC
GAAGGCGATG
CTACATACGG
CAAACTTACA
CTTAAATTCA
TCTGCACAAC
AGGCAAACTT
CCTGTTCCTT
GGCCTACACT
TGTTACAACA
TTCACATACG
GCGTTCAATG
CTTCTCTCGT
TACCCTGATC
ATATGAAACA
ACATGATTTC
TTCAAATCTG
CTATGCCTGA
AGGCTACGTT
CAAGAACGTA
CAATCTTCTT
CAAAGATGAT
GGCAACTACA
AAACACGTGC
TGAAGTTAAA
TTCGAAGGCG
ATACACTTGT
TAACCGTATC
GAACTTAAAG
GCATCGATTT
CAAAGAAGAT
GGCAACATCC
TTGGCCATAA
ACTTGAATAC
AACTACAACT
CTCATAACGT
TTACATCATG
GCTGATAAAC
AAAAAAACGG
CATCAAAGTT
AACTTCAAAA
TCCGTCATAA
CATCGAAGAT
GGCTCTGTTC
AACTTGCTGA
TCATTACCAA
CAAAACACAC
CTATCGGCGA
TGGCCCTGTT
CTTCTTCCTG
ATAACCATTA
CCTTTCTACA
CAATCTGCTC
TTTCTAAAGA
TCCTAACGAA
AAACGTGATC
ATATGGTTCT
TCTTGAATTC
GTTACAGCTG
CTGGCATCAC
ACATGGCATG
GATGAACTTT
ACAAATAATC
TAGAGTCGAC
GTCCCCGGGG
CAGCCCGCCT
AATGAGCGGG
CTTTTTTCAC
GTCACGCGTC
CATGGAGATC
TTTGTCTGCA
ACTGAAAAGT
TTATACCTTA
CCTGGAACAA
ATGGTTGAAA
CATACGAGGC
TAATATCGGC
TTATTAGGAA
TAGTCCCTGT
ACTAATAAAA
TCAGGTGGAT
CAGTTGATCA
GTATATTTTG
GACGAAGCTC
GGAAAGAATT
TGGAGATGAC
TTGCTTAATT
CCACAATTAA
ATTAAGGGAA
AGAATAAAGC
GATTTGATGT
TCAAGGAATC
ACGGAAGAAG
ATACTCATGA
TAAAGAAGCT
CTAAAACTAT
TCAATAACCT
TACAATGGAA
TTGATCGAAA
GGGTGGAAGG
TTAATGGTAC
GAAAATTAGG
GGATCTACCT
AGAAAGCCAC
AAGGCGATAG
GTCAAGCTTA
AAGAACCCTT
ACATGGATCT
TACAGATTCT
GAAAGTAAAG
AAACAACAGA
GGTTAAACAA
ACAGAACCAA
AAAGAAAAAA
AGCATTGTTG
AAAACAATGA
AAGTTGATGT
TTCAATCCAT
AATAAGATTA
AATCGCTGCA
CGAAATTCTG
GCAGCATCCG
AAGGGAATTC
ATATTACTTA
GAGGATACTA
TTGAGAGAGC
TATTGATAAG
ATGGTTGAGA
CATTACCTGA
GAGCCAAAAA
ACTTTTTATG
AATATGAATT
AAAAAAAAGA
ACCAACAAAG
GCTGAGACAG
ACTCCAAACG
AGTCTGTTTT
TTTAAAAAAA
ATATTAGGAG
CATTGAATAT
ATATTAGAGA
ATTAAGAAAG
ACATGGGAAT
AAAAATATTT
TAAATCCAGT
AAAAATATGA
TAAGATTATT
TCAGAATATG
AAGAACTCTG
TTTGTTTTTG
ATGAAAAAAC
AAACAAAAAA
AATCCACCTA
ACGGAATCTC
AATTTAACTA
ACAGCGGCCA
AACTGAGAAG
TTAAATTTGA
GAAGGGGAAA
AGGCGGATTT
ATACTTGTAT
TTAACTATCT
CCATTTTAAC
ATTTTATTAA
ACCCCATACA
AGTGAAAATC
CTCTTTTACA
CTGTTCCTTT
AGGTGATCGC
GGAGGGACAT
TATGAGTGAA
GTAAACCTAA
AAGGAAATAC
AGATGAATTA
GTGTATTATC
GACAGCAAAC
CACTGGAAAT
AAAATCGCCA
GGAAGAGAAT
CAAAAAAGGG
AAAGAAGAAG
TTTATTATGT
TGCTGAAACG
GAAGAGAAGA
TATGGACAGA
AGAGCAAATA
AAAAACTTTT
CTTTAGACAA
ATTTGGTACG
CATATACCTT
ACATAGAAGG
TCATTATACA
ATCTTAAATA
ATTACTTCTT
TGATTTTTGG
GGCTATTTTT
TAGGTGCTGA
AGGAATTGCG
CTCTATGCTC
ACCTAACTCG
TTATGCATAC
GGCAGCAAAG
ACTTTTGCTT
TCCTAGTCTA
CAAACAATCG
CTAAAAAAAT
GGACAAGACT
CCTGTTACAG
TTAGAGGCTA
CTTGAAACTG
CTTGAAAGGT
ACGGTTTTAT
TTGGAAGGTA
AACGTCCGTA
ATAAAACCAA
GGATAACACA
GAGGAATCCC
CGATTTTTAA
GATTAGACGT
AAGGTTCCTT
TGCTTTCAGA
AGAACTTTTA
AATGGAAACC
CTAATATTGA
AATTCCAGAT
GACGAGGAAG
CACATGTAAA
GAAGGCTTTA
AAAAAGGAAA
AAGAGGGTCT
TCCAAAGGTT
TTGAAAAAAG
AGCACGATGA
ATTTGTTAAA
AAAATGATGG
ATGAGTCAGA
AACAATTAAT
ATTCCAGAGG
CCTTACAATA
TGACACAATG
TATGAAGATA
TACTCAGTAA
AGGAGAAATT
CGAAAAGAAA
TCAAAAAACA
AATACCTAAT
CCTACAACAT
CTTTTGAGAG
TATATCAATG
ACAACTGAAG
AGGAAAAAGT
CGACAGTACT
TTAAAAAGCG
AAATGCAAAA
TCGTGTCTCT
AAGCCTTCTT
TTGATACCTG
GTTTAAAAAC
ACTAAGATCA
AAATTGAAAA
TAAAAATTGT
TTATTACTTG
TACCGAGTGA
ATTTGCATTT
GAATGGATTA
AGAAAAGATA
TTTAGAAACA
ATTAAAACAG
TCCTTGAAGA
AGCTGGATAT
GTTTTCGAAA
AAATCGAACT
AAGAAAAGTG
CAATAAACTG
CTGAAGTATT
TCAGCAGTTT
TTTTTATTTA
GAAATAGTGA
AAAAAATATA
ATCAGGGAGG
TATCAATATT
TAATGAGTAC
TGATTTAAAT
TTATTTAGAC
TGGAATTAAT
AATTAACACG
TAGACTAATT
AAAATTTAAT
GAGGGATAAA
GAGGATACAA
AAATATTAAT
TTCAATCCCT
ATTAAATTTT
AACAAGGGGG
GGATTAAAAT
TTAATTAGAG
GTTTATCCAC
AAGAAAAGAC
CCTAATAAAA
TTTTTACTAG
GGTTATAACA
CTGATTAATT
TCTTAATGGG
GGAGGGATTA
AAATTTAATG
ACAAAGAAAA
CAATCTTTTA
AGAAAAGCTT
TTAAAAGATA
ATAATAAAAA
GAGCTTTGCG
ATTAAGCAAA
ACTCTTTACT
TTTTCATTGA
CATTATCAAA
TTCATCGATT
TCAAATTGTT
GTTGTATCAT
AAAGTTAATT
CTGTTTTGCA
CAACCTTTTC
AGGAATATAA
AACACATCTG
AGGCTTGTTT
TATAAACTCA
GGGTCGCTAA
AGTCAATGTA
ACGTAGCATA
TGATATGGTA
TAGCTTCCAC
CCAAGTTAGC
CTTTCTGCTT
CTTCTGAATG
TTTTTCATAT
ACTTCCATGG
GTATCTCTAA
ATGATTTTCC
TCATGTAGCA
AGGTATGAGC
AAAAAGTTTA
TGGAATTGAT
AGTTCCTCTC
TTTTTCTTCA
ACTTTTTTAT
CTAAAACAAA
CACTTTAACA
TCTGAGTCAA
TGTAAGCATA
AGATGTTTTT
CCAGTCATAA
TTTCAATCCC
AAATCTTTTA
GACAGAAATT
CTGGACGTAA
ATCTTTTGGT
GAAAGAATTT
TTTTATGTAG
CAATATATCC
GATACAGCAC
CTTCTAAAAG
CGTTGGTGAA
TAGGGCATTT
TACCTATCTC
CTCTCATTTT
GTGGAATAAA
AATAGTCATA
TTCGTCCATC
TACCTATCCT
ATTATCGAAC
AGTTGAACTT
TTTAATCAAG
GATCAGTCCT
TTTTTTCATT
ATTCTTAAAC
TGTGCTCTTA
ACTTTAACAA
CTCGATTTGT
TTTTCCAGAT
CTCGAGGGTA
ACTAGCCTCG
CCGATCCCGC
AAGAGGCCCG
GCAGTCAGGT
GGCACTTTTC
GGGGAAATGT
GCGCGGAACC
CCTATTTGTT
TATTTTTCTA
AATACATTCA
AATATGTATC
CGCTCATGAG
ACAATAACCC
TGATAAATGC
TTCAATAATA
TTGAAAAAGG
AAGAGTATGA
GTATTCAACA
TTTCCGTGTC
GCCCTTATTC
CCTTTTTTGC
GGCATTTTGC
CTTCCTGTTT
TTGCTCACCC
AGAAACGCTG
GTGAAAGTAA
AAGATGCTGA
AGATCAGTTG
GGTGCACGAG
TGGGTTACAT
CGAACTGGAT
CTCAACAGCG
GTAAGATCCT
TGAGAGTTTT
CGCCCCGAAG
AACGTTTTCC
AATGATGAGC
ACTTTTAAAG
TTCTGCTATG
TGGCGCGGTA
TTATCCCGTA
TTGACGCCGG
GCAAGAGCAA
CTCGGTCGCC
GCATACACTA
TTCTCAGAAT
GACTTGGTTG
AGTACTCACC
AGTCACAGAA
AAGCATCTTA
CGGATGGCAT
GACAGTAAGA
GAATTATGCA
GTGCTGCCAT
AACCATGAGT
GATAACACTG
CGGCCAACTT
ACTTCTGACA
ACGATCGGAG
GACCGAAGGA
GCTAACCGCT
TTTTTGCACA
ACATGGGGGA
TCATGTAACT
CGCCTTGATC
GTTGGGAACC
GGAGCTGAAT
GAAGCCATAC
CAAACGACGA
GCGTGACACC
ACGATGCCTG
TAGCAATGGC
AACAACGTTG
CGCAAACTAT
TAACTGGCGA
ACTACTTACT
CTAGCTTCCC
GGCAACAATT
AATAGACTGG
ATGGAGGCGG
ATAAAGTTGC
AGGACCACTT
CTGCGCTCGG
CCCTTCCGGC
TGGCTGGTTT
ATTGCTGATA
AATCTGGAGC
CGGTGAGCGT
GGGTCTCGCG
GTATCATTGC
AGCACTGGGG
CCAGATGGTA
AGCCCTCCCG
TATCGTAGTT
ATCTACACGA
CGGGGAGTCA
GGCAACTATG
GATGAACGAA
ATAGACAGAT
CGCTGAGATA
GGTGCCTCAC
TGATTAAGCA
TTGGTAACTG
TCAGACCAAG
TTTACTCATA
TATACTTTAG
ATTGATTTAA
AACTTCATTT
TTAATTTAAA
AGGATCTAGG
TGAAGATCCT
TTTTGATAAT
CTCATGACCA
AAATCCCTTA
ACGTGAGTTT
TCGTTCCACT
GAGCGTCAGA
CCCCGTAGAA
AAGATCAAAG
GATCTTCTTG
AGATCCTTTT
TTTCTGCGCG
TAATCTGCTG
CTTGCAAACA
AAAAAACCAC
CGCTACCAGC
GGTGGTTTGT
TTGCCGGATC
AAGAGCTACC
AACTCTTTTT
CCGAAGGTAA
CTGGCTTCAG
CAGAGCGCAG
ATACCAAATA
CTGTCCTTCT
AGTGTAGCCG
TAGTTAGGCC
ACCACTTCAA
GAACTCTGTA
GCACCGCCTA
CATACCTCGC
TCTGCTAATC
CTGTTACCAG
TGGCTGCTGC
CAGTGGCGAT
AAGTCGTGTC
TTACCGGGTT
GGACTCAAGA
CGATAGTTAC
CGGATAAGGC
GCAGCGGTCG
GGCTGAACGG
GGGGTTCGTG
CACACAGCCC
AGCTTGGAGC
GAACGACCTA
CACCGAACTG
AGATACCTAC
AGCGTGAGCT
ATGAGAAAGC
GCCACGCTTC
CCGAAGGGAG
AAAGGCGGAC
AGGTATCCGG
TAAGCGGCAG
GGTCGGAACA
GGAGAGCGCA
CGAGGGAGCT
TCCAGGGGGA
AACGCCTGGT
ATCTTTATAG
TCCTGTCGGG
TTTCGCCACC
TCTGACTTGA
GCGTCGATTT
TTGTGATGCT
CGTCAGGGGG
GCGGAGCCTA
TGGAAAAACG
CCAGCAACGC
GGCCTTTTTA
CGGTTCCTGG
CCTTTTGCTG
GCCTTTTGCT
CACATGTTCT
TTCCTGCGTT
ATCCCCTGAT
TCTGTGGATA
ACCGTATTAC
CGCCTTTGAG
TGAGCTGATA
CCGCTCGCCG
CAGCCGAACG
ACCGAGCGCA
GCGAGTCAGT
GAGCGAGGAA
GCGGAAGAGC
GCCCAATACG
CATGC