名称: | pmirGLO-3'UTR-TNF |
---|---|
别称: | Plasmid#153069 |
基因长: | 774bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 启动子检测 |
片段类型: | UTR |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | Fluc,Rluc |
启动子: | PGK |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | P0198 |
正向引物: | PmirGLO-F:GACGAGGTGCCTAAAGGACT |
反向引物: | pCMV-R:TCCAAACTCATCAATGTATC |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CAGAGGCTGG
GAAGGGGTGG
GTCCGGGGGC
GGGCTCAGGG
GCGGGCTCAG
GGGCGGGGCG
GGCGCCCGAA
GGTCCTCCGG
AGGCCCGGCA
TTCTGCACGC
TTCAAAAGCG
CACGTCTGCC
GCGCTGTTCT
CCTCTTCCTC
ATCTCCGGGC
CTTTCGACCT
GCAGCCCAAG
CTTGGCAATC
CGGTACTGTT
GGTAAAGCCA
CCATGGAAGA
TGCCAAAAAC
ATTAAGAAGG
GCCCAGCGCC
ATTCTACCCA
CTCGAAGACG
GGACCGCCGG
CGAGCAGCTG
CACAAAGCCA
TGAAGCGCTA
CGCCCTGGTG
CCCGGCACCA
TCGCCTTTAC
CGACGCACAT
ATCGAGGTGG
ACATTACCTA
CGCCGAGTAC
TTCGAGATGA
GCGTTCGGCT
GGCAGAAGCT
ATGAAGCGCT
ATGGGCTGAA
TACAAACCAT
CGGATCGTGG
TGTGCAGCGA
GAATAGCTTG
CAGTTCTTCA
TGCCCGTGTT
GGGTGCCCTG
TTCATCGGTG
TGGCTGTGGC
CCCAGCTAAC
GACATCTACA
ACGAGCGCGA
GCTGCTGAAC
AGCATGGGCA
TCAGCCAGCC
CACCGTCGTA
TTCGTGAGCA
AGAAAGGGCT
GCAAAAGATC
CTCAACGTGC
AAAAGAAGCT
ACCGATCATA
CAAAAGATCA
TCATCATGGA
TAGCAAGACC
GACTACCAGG
GCTTCCAAAG
CATGTACACC
TTCGTGACTT
CCCATTTGCC
ACCCGGCTTC
AACGAGTACG
ACTTCGTGCC
CGAGAGCTTC
GACCGGGACA
AAACCATCGC
CCTGATCATG
AACAGTAGTG
GCAGTACCGG
ATTGCCCAAG
GGCGTAGCCC
TACCGCACCG
CACCGCTTGT
GTCCGATTCA
GTCATGCCCG
CGACCCCATC
TTCGGCAACC
AGATCATCCC
CGACACCGCT
ATCCTCAGCG
TGGTGCCATT
TCACCACGGC
TTCGGCATGT
TCACCACGCT
GGGCTACTTG
ATCTGCGGCT
TTCGGGTCGT
GCTCATGTAC
CGCTTCGAGG
AGGAGCTATT
CTTGCGCAGC
TTGCAAGACT
ATAAGATTCA
ATCTGCCCTG
CTGGTGCCCA
CACTATTTAG
CTTCTTCGCT
AAGAGCACTC
TCATCGACAA
GTACGACCTA
AGCAACTTGC
ACGAGATCGC
CAGCGGCGGG
GCGCCGCTCA
GCAAGGAGGT
AGGTGAGGCC
GTGGCCAAAC
GCTTCCACCT
ACCAGGCATC
CGCCAGGGCT
ACGGCCTGAC
AGAAACAACC
AGCGCCATTC
TGATCACCCC
CGAAGGGGAC
GACAAGCCTG
GCGCAGTAGG
CAAGGTGGTG
CCCTTCTTCG
AGGCTAAGGT
GGTGGACTTG
GACACCGGTA
AGACACTGGG
TGTGAACCAG
CGCGGCGAGC
TGTGCGTCCG
TGGCCCCATG
ATCATGAGCG
GCTACGTTAA
CAACCCCGAG
GCTACAAACG
CTCTCATCGA
CAAGGACGGC
TGGCTGCACA
GCGGCGACAT
CGCCTACTGG
GACGAGGACG
AGCACTTCTT
CATCGTGGAC
CGGCTGAAGA
GCCTGATCAA
ATACAAGGGC
TACCAGGTAG
CCCCAGCCGA
ACTGGAGAGC
ATCCTGCTGC
AACACCCCAA
CATCTTCGAC
GCCGGGGTCG
CCGGCCTGCC
CGACGACGAT
GCCGGCGAGC
TGCCCGCCGC
AGTCGTCGTG
CTGGAACACG
GTAAAACCAT
GACCGAGAAG
GAGATCGTGG
ACTATGTGGC
CAGCCAGGTT
ACAACCGCCA
AGAAGCTGCG
CGGTGGTGTT
GTGTTCGTGG
ACGAGGTGCC
TAAAGGACTG
ACCGGCAAGT
TGGACGCCCG
CAAGATCCGC
GAGATTCTCA
TTAAGGCCAA
GAAGGGCGGC
AAGATCGCCG
TGTAATTCTA
GTTGTTTAAA
CGAGCTCGCT
AGCGGAGGAC
GAACATCCAA
CCTTCCCAAA
CGCCTCCCCT
GCCCCAATCC
CTTTATTACC
CCCTCCTTCA
GACACCCTCA
ACCTCTTCTG
GCTCAAAAAG
AGAATTGGGG
GCTTAGGGTC
GGAACCCAAG
CTTAGAACTT
TAAGCAACAA
GACCACCACT
TCGAAACCTG
GGATTCAGGA
ATGTGTGGCC
TGCACAGTGA
AGTGCTGGCA
ACCACTAAGA
ATTCAAACTG
GGGCCTCCAG
AACTCACTGG
GGCCTACAGC
TTTGATCCCT
GACATCTGGA
ATCTGGAGAC
CAGGGAGCCT
TTGGTTCTGG
CCAGAATGCT
GCAGGACTTG
AGAAGACCTC
ACCTAGAAAT
TGACACAAGT
GGACCTTAGG
CCTTCCTCTC
TCCAGATGTT
TCCAGACTTC
CTTGAGACAC
GGAGCCCAGC
CCTCCCCATG
GAGCCAGCTC
CCTCTATTTA
TGTTTGCACT
TGTGATTATT
TATTATTTAT
TTATTATTTA
TTTATTTACA
GATGAATGTA
TTTATTTGGG
AGACCGGGGT
ATCCTGGGGG
ACCCAATGTA
GGAGCTGCCT
TGGCTCAGAC
ATGTTTTCCG
TGAAAACGGA
GCTGAACAAT
AGGCTGTTCC
CATGTAGCCC
CCTGGCCTCT
GTGCCTTCTT
TTGATTATGT
TTTTTAAAAT
ATTTATCTGA
TTAAGTTGTC
TAAACAATGC
TGATTTGGTG
ACCAACTGTC
ACTCATTGCT
GAGCCTCTGC
TCCCCAGGGG
AGTTGTGTCT
GTAATCGCCC
TACTATTCAG
TGGCGAGATC
TAGAGTCGAC
CTGCAGGCAT
GCAAGCTGAT
CCGGCTGCTA
ACAAAGCCCG
AAAGGAAGCT
GAGTTGGCTG
CTGCCACCGC
TGAGCAATAA
CTAGCATAAC
CCCTTGGGGC
GGCCGCTTCG
AGCAGACATG
ATAAGATACA
TTGATGAGTT
TGGACAAACC
ACAACTAGAA
TGCAGTGAAA
AAAATGCTTT
ATTTGTGAAA
TTTGTGATGC
TATTGCTTTA
TTTGTAACCA
TTATAAGCTG
CAATAAACAA
GTTAACAACA
ACAATTGCAT
TCATTTTATG
TTTCAGGTTC
AGGGGGAGAT
GTGGGAGGTT
TTTTTAAGCA
AGTAAAACCT
CTACAAATGT
GGTAAAATCG
AATTTTAACA
AAATATTAAC
GCTTACAATT
TCCTGATGCG
GTATTTTCTC
CTTACGCATC
TGTGCGGTAT
TTCACACCGC
ATACGCGGAT
CTGCGCAGCA
CCATGGCCTG
AAATAACCTC
TGAAAGAGGA
ACTTGGTTAG
GTACCTTCTG
AGGCGGAAAG
AACCAGCTGT
GGAATGTGTG
TCAGTTAGGG
TGTGGAAAGT
CCCCAGGCTC
CCCAGCAGGC
AGAAGTATGC
AAAGCATGCA
TCTCAATTAG
TCAGCAACCA
GGTGTGGAAA
GTCCCCAGGC
TCCCCAGCAG
GCAGAAGTAT
GCAAAGCATG
CATCTCAATT
AGTCAGCAAC
CATAGTCCCG
CCCCTAACTC
CGCCCATCCC
GCCCCTAACT
CCGCCCAGTT
CCGCCCATTC
TCCGCCCCAT
GGCTGACTAA
TTTTTTTTAT
TTATGCAGAG
GCCGAGGCCG
CCTCGGCCTC
TGAGCTATTC
CAGAAGTAGT
GAGGAGGCTT
TTTTGGAGGC
CTAGGCTTTT
GCAAAAAGCT
TGATTCTTCT
GACACAACAG
TCTCGAACCA
AAGGCTGGAG
CCACCATGGC
TTCCAAGGTG
TACGACCCCG
AGCAACGCAA
ACGCATGATC
ACTGGGCCTC
AGTGGTGGGC
TCGCTGCAAG
CAAATGAACG
TGCTGGACTC
CTTCATCAAC
TACTATGATT
CCGAGAAGCA
CGCCGAGAAC
GCCGTGATTT
TTCTGCATGG
TAACGCTGCC
TCCAGCTACC
TGTGGAGGCA
CGTCGTGCCT
CACATCGAGC
CCGTGGCTAG
ATGCATCATC
CCTGATCTGA
TCGGAATGGG
TAAGTCCGGC
AAGAGCGGGA
ATGGCTCATA
TCGCCTCCTG
GATCACTACA
AGTACCTCAC
CGCTTGGTTC
GAGCTGCTGA
ACCTTCCAAA
GAAAATCATC
TTTGTGGGCC
ACGACTGGGG
GGCTTGTCTG
GCCTTTCACT
ACTCCTACGA
GCACCAAGAC
AAGATCAAGG
CCATCGTCCA
TGCTGAGAGT
GTCGTGGACG
TGATCGAGTC
CTGGGACGAG
TGGCCTGACA
TCGAGGAGGA
TATCGCCCTG
ATCAAGAGCG
AAGAGGGCGA
GAAAATGGTG
CTTGAGAATA
ACTTCTTCGT
CGAGACCATG
CTCCCAAGCA
AGATCATGCG
GAAACTGGAG
CCTGAGGAGT
TCGCTGCCTA
CCTGGAGCCA
TTCAAGGAGA
AGGGCGAGGT
TAGACGGCCT
ACCCTCTCCT
GGCCTCGCGA
GATCCCTCTC
GTTAAGGGAG
GCAAGCCCGA
CGTCGTCCAG
ATTGTCCGCA
ACTACAACGC
CTACCTTCGG
GCCAGCGACG
ATCTGCCTAA
GATGTTCATC
GAGTCCGACC
CTGGGTTCTT
TTCCAACGCT
ATTGTCGAGG
GAGCTAAGAA
GTTCCCTAAC
ACCGAGTTCG
TGAAGGTGAA
GGGCCTCCAC
TTCAGCCAGG
AGGACGCTCC
AGATGAAATG
GGTAAGTACA
TCAAGAGCTT
CGTGGAGCGC
GTGCTGAAGA
ACGAGCAGAC
CGGTGGTGGG
AGCGGAGGTG
GCGGATCAGG
TGGCGGAGGC
TCCGGAGGGA
TTGAACAAGA
TGGATTGCAC
GCAGGTTCTC
CGGCCGCTTG
GGTGGAGAGG
CTATTCGGCT
ATGACTGGGC
ACAACAGACA
ATCGGCTGCT
CTGATGCCGC
CGTGTTCCGG
CTGTCAGCGC
AGGGGCGCCC
GGTTCTTTTT
GTCAAGACCG
ACCTGTCCGG
TGCCCTGAAT
GAACTGCAGG
ACGAGGCAGC
GCGGCTATCG
TGGCTGGCCA
CGACGGGCGT
TCCTTGCGCA
GCTGTGCTCG
ACGTTGTCAC
TGAAGCGGGA
AGGGACTGGC
TGCTATTGGG
CGAAGTGCCG
GGGCAGGATC
TCCTGTCATC
TCACCTTGCT
CCTGCCGAGA
AAGTATCCAT
CATGGCTGAT
GCAATGCGGC
GGCTGCATAC
GCTTGATCCG
GCTACCTGCC
CATTCGACCA
CCAAGCGAAA
CATCGCATCG
AGCGAGCACG
TACTCGGATG
GAAGCCGGTC
TTGTCGATCA
GGATGATCTG
GACGAAGAGC
ATCAGGGGCT
CGCGCCAGCC
GAACTGTTCG
CCAGGCTCAA
GGCGCGCATG
CCCGACGGCG
AGGATCTCGT
CGTGACCCAT
GGCGATGCCT
GCTTGCCGAA
TATCATGGTG
GAAAATGGCC
GCTTTTCTGG
ATTCATCGAC
TGTGGCCGGC
TGGGTGTGGC
GGACCGCTAT
CAGGACATAG
CGTTGGCTAC
CCGTGATATT
GCTGAAGAGC
TTGGCGGCGA
ATGGGCTGAC
CGCTTCCTCG
TGCTTTACGG
TATCGCCGCT
CCCGATTCGC
AGCGCATCGC
CTTCTATCGC
CTTCTTGACG
AGTTCTTCTG
AGCGGGACTC
TGGGGTTCGA
AATGACCGAC
CAAGCGACGC
CCAACCTGCC
ATCACGATGG
CCGCAATAAA
ATATCTTTAT
TTTCATTACA
TCTGTGTGTT
GGTTTTTTGT
GTGAATCGAT
AGCGATAAGG
ATCCTCTTTG
CGCTTGCGTT
TTCCCTTGTC
CAGATAGCCC
AGTAGCTGAC
ATTCATCCGG
GGTCAGCACC
GTTTCTGCGG
ACTGGCTTTC
TACGTAATGG
TTTCTTAGAC
GTCAGGTGGC
ACTTTTCGGG
GAAATGTGCG
CGGAACCCCT
ATTTGTTTAT
TTTTCTAAAT
ACATTCAAAT
ATGTATCCGC
TCATGAGACA
ATAACCCTGA
TAAATGCTTC
AATAATATTG
AAAAAGGAAG
AGTATGAGTA
TTCAACATTT
CCGTGTCGCC
CTTATTCCCT
TTTTTGCGGC
ATTTTGCCTT
CCTGTTTTTG
CTCACCCAGA
AACGCTGGTG
AAAGTAAAAG
ATGCTGAAGA
TCAGTTGGGT
GCACGAGTGG
GTTACATCGA
ACTGGATCTC
AACAGCGGTA
AGATCCTTGA
GAGTTTTCGC
CCCGAAGAAC
GTTTTCCAAT
GATGAGCACT
TTCAAAGTTC
TGCTATGTGG
CGCGGTATTA
TCCCGTATTG
ACGCCGGGCA
AGAGCAACTC
GGTCGCCGCA
TACACTATTC
TCAGAATGAC
TTGGTTGAGT
ACTCACCAGT
CACAGAAAAG
CATCTTACGG
ATGGCATGAC
AGTAAGAGAA
TTATGCAGTG
CTGCCATAAC
CATGAGTGAT
AACACTGCGG
CCAACTTACT
TCTGACAACT
ATCGGAGGAC
CGAAGGAGCT
AACCGCTTTT
TTGCACAACA
TGGGGGATCA
TGTAACTCGC
CTTGATCGTT
GGGAACCGGA
GCTGAATGAA
GCCATACCAA
ACGACGAGCG
TGACACCACG
ATGCCTGTAG
CAATGGCAAC
AACGTTGCGC
AAACTATTAA
CTGGCGAACT
ACTTACTCTA
GCTTCCCGGC
AACAATTAAT
AGACTGGATG
GAGGCGGATA
AAGTTGCAGG
ACCACTTCTG
CGCTCGGCCC
TTCCGGCTGG
CTGGTTTATT
GCTGATAAAT
CTGGAGCCGG
TGAGCGTGGG
TCTCGCGGTA
TCATTGCAGC
ACTGGGGCCA
GATGGTAAGC
CCTCCCGTAT
CGTAGTTATC
TACACGACGG
GGAGTCAGGC
AACTATGGAT
GAACGAAATA
GACAGATCGC
TGAGATAGGT
GCCTCACTGA
TTAAGCATTG
GTAATTCGAA
ATGACCGACC
AAGCGACGCC
CAACCGGTAT
CAGCTCACTC
AAAGGCGGTA
ATACGGTTAT
CCACAGAATC
AGGGGATAAC
GCAGGAAAGA
ACATGTGAGC
AAAAGGCCAG
CAAAAGGCCA
GGAACCGTAA
AAAGGCCGCG
TTGCTGGCGT
TTTTCCATAG
GCTCCGCCCC
CCTGACGAGC
ATCACAAAAA
TCGACGCTCA
AGTCAGAGGT
GGCGAAACCC
GACAGGACTA
TAAAGATACC
AGGCGTTTCC
CCCTGGAAGC
TCCCTCGTGC
GCTCTCCTGT
TCCGACCCTG
CCGCTTACCG
GATACCTGTC
CGCCTTTCTC
CCTTCGGGAA
GCGTGGCGCT
TTCTCATAGC
TCACGCTGTA
GGTATCTCAG
TTCGGTGTAG
GTCGTTCGCT
CCAAGCTGGG
CTGTGTGCAC
GAACCCCCCG
TTCAGCCCGA
CCGCTGCGCC
TTATCCGGTA
ACTATCGTCT
TGAGTCCAAC
CCGGTAAGAC
ACGACTTATC
GCCACTGGCA
GCAGCCACTG
GTAACAGGAT
TAGCAGAGCG
AGGTATGTAG
GCGGTGCTAC
AGAGTTCTTG
AAGTGGTGGC
CTAACTACGG
CTACACTAGA
AGGACAGTAT
TTGGTATCTG
CGCTCTGCTG
AAGCCAGTTA
CCTTCGGAAA
AAGAGTTGGT
AGCTCTTGAT
CCGGCAAACA
AACCACCGCT
GGTAGCGGTG
GTTTTTTTGT
TTGCAAGCAG
CAGATTACGC
GCAGAAAAAA
AGGATTTCAA
GAAGATCCTT
TGATCTTTTC
TACGGGGTCT
GACGCTCAGT
GGAACGAAAA
CTCACGTTAA
GGGATTTTGG
TCATGAGATT
ATCAAAAAGG
ATCTTCACCT
AGATCCTTTT
ATAGTCCGGA
AATACAGGAA
CGCACGCTGG
ATGGCCCTTC
GCTGGGATGG
TGAAACCATG
AAAAATGGCA
GCTTCAGTGG
ATTAAGTGGG
GGTAATGTGG
CCTGTACCCT
CTGGTTGCAT
AGGTATTCAT
ACGGTTAAAA
TTTATCAGGC
GCGATTGCGG
CAGTTTTTCG
GGTGGTTTGT
TGCCATTTTT
ACCTGTCTGC
TGCCGTGATC
GCGCTGAACG
CGTTTTAGCG
GTGCGTACAA
TTAAGGGATT
ATGGTAAATC
CACTTACTGT
CTGCCCTCGT
AGCCATCGAG
ATAAACCGCA
GTACTCCGGC
CACGATGCGT
CCGGCGTAGA
GGATCGAGAT
CTACCGGGTA
GGGGAGGCGC
TTTTCCCAAG
GCAGTCTGGA
GCATGCGCTT
TAGCAGCCCC
GCTGGGCACT
TGGCGCTACA
CAAGTGGCCT
CTGGCCTCGC
ACACATTCCA
CATCCACCGG
TAGGCGCCAA
CCGGCTCCGT
TCTTTGGTGG
CCCCTTCGCG
CCACCTTCTA
CTCCTCCCCT
AGTCAGGAAG
TTCCCCCCCG
CCCCGCAGCT
CGCGTCGTGC
AGGACGTGAC
AAATGGAAGT
AGCACGTCTC
ACTAGTCTCG
TGCAGATGGA
CAGCACCGCT
GAGCAATGGA
AGCGGGTAGG
CCTTTGGGGC
AGCGGCCAAT
AGCAGCTTTG
CTCCTTCGCT
TTCTGGGCT