名称: | pAtCas9-dead_1 |
---|---|
别称: | Plasmid#91165 |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Kan |
筛选标记: | Hyg |
荧光标记: | |
启动子: | 35S |
复制子: | pVS1 |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTAGGCGGTG
CTACAGAGTT
CTTGAAGTGG
TGGCCTAACT
ACGGCTACAC
TAGAAGGACA
GTATTTGGTA
TCTGCGCTCT
GCTGAAGCCA
GTTACCTTCG
GAAAAAGAGT
TGGTAGCTCT
TGATCCGGCA
AACAAACCAC
CGCTGGTAGC
GGTGGTTTTT
TTGTTTGCAA
GCAGCAGATT
ACGCGCAGAA
AAAAAGGATC
TCAAGAAGAT
CCTTTGATCT
TTTCTACGGG
GTCTGACGCT
CAGTGGAACG
AAAACTCACG
TTAAGGGATT
TTGGTCATGC
ATTCTAGGTA
CTAAAACAAT
TCATCCAGTA
AAATATAATA
TTTTATTTTC
TCCCAATCAG
GCTTGATCCC
CAGTAAGTCA
AAAAATAGCT
CGACATACTG
TTCTTCCCCG
ATATCCTCCC
TGATCGACCG
GACGCAGAAG
GCAATGTCAT
ACCACTTGTC
CGCCCTGCCG
CTTCTCCCAA
GATCAATAAA
GCCACTTACT
TTGCCATCTT
TCACAAAGAT
GTTGCTGTCT
CCCAGGTCGC
CGTGGGAAAA
GACAAGTTCC
TCTTCGGGCT
TTTCCGTCTT
TAAAAAATCA
TACAGCTCGC
GCGGATCTTT
AAATGGAGTG
TCTTCTTCCC
AGTTTTCGCA
ATCCACATCG
GCCAGATCGT
TATTCAGTAA
GTAATCCAAT
TCGGCTAAGC
GGCTGTCTAA
GCTATTCGTA
TAGGGACAAT
CCGATATGTC
GATGGAGTGA
AAGAGCCTGA
TGCACTCCGC
ATACAGCTCG
ATAATCTTTT
CAGGGCTTTG
TTCATCTTCA
TACTCTTCCG
AGCAAAGGAC
GCCATCGGCC
TCACTCATGA
GCAGATTGCT
CCAGCCATCA
TGCCGTTCAA
AGTGCAGGAC
CTTTGGAACA
GGCAGCTTTC
CTTCCAGCCA
TAGCATCATG
TCCTTTTCCC
GTTCCACATC
ATAGGTGGTC
CCTTTATACC
GGCTGTCCGT
CATTTTTAAA
TATAGGTTTT
CATTTTCTCC
CACCAGCTTA
TATACCTTAG
CAGGAGACAT
TCCTTCCGTA
TCTTTTACGC
AGCGGTATTT
TTCGATCAGT
TTTTTCAATT
CCGGTGATAT
TCTCATTTTA
GCCATTTATT
ATTTCCTTCC
TCTTTTCTAC
AGTATTTAAA
GATACCCCAA
GAAGCTAATT
ATAACAAGAC
GAACTCCAAT
TCACTGTTCC
TTGCATTCTA
AAACCTTAAA
TACCAGAAAA
CAGCTTTTTC
AAAGTTGTTT
TCAAAGTTGG
CGTATAACAT
AGTATCGACG
GAGCCGATTT
TGAAACCGCG
GTGATCACAG
GCAGCAACGC
TCTGTCATCG
TTACAATCAA
CATGCTACCC
TCCGCGAGAT
CATCCGTGTT
TCAAACCCGG
CAGCTTAGTT
GCCGTTCTTC
CGAATAGCAT
CGGTAACATG
AGCAAAGTCT
GCCGCCTTAC
AACGGCTCTC
CCGCTGACGC
CGTCCCGGAC
TGATGGGCTG
CCTGTATCGA
GTGGTGATTT
TGTGCCGAGC
TGCCGGTCGG
GGAGCTGTTG
GCTGGCTGGT
GGCAGGATAT
ATTGTGGTGT
AAACAAATTG
ACGCTTAGAC
AACTTAATAA
CACATTGCGG
ACGTTTTTAA
TGTACTGAAT
TAACGCCGAA
TTAATTCGGG
GGATCTGGAT
TTTAGTACTG
GATTTTGGTT
TTAGGAATTA
GAAATTTTAT
TGATAGAAGT
ATTTTACAAA
TACAAATACA
TACTAAGGGT
TTCTTATATG
CTCAACACAT
GAGCGAAACC
CTATAGGAAC
CCTAATTCCC
TTATCTGGGA
ACTACTCACA
CATTATTATG
GAGAAACTCG
AGCTTGTCGA
TCGACAGATC
CCGGTCGGCA
TCTACTCTAT
TTCTTTGCCC
TCGGACGAGT
GCTGGGGCGT
CGGTTTCCAC
TATCGGCGAG
TACTTCTACA
CAGCCATCGG
TCCAGACGGC
CGCGCTTCTG
CGGGCGATTT
GTGTACGCCC
GACAGTCCCG
GCTCCGGATC
GGACGATTGC
GTCGCATCGA
CCCTGCGCCC
AAGCTGCATC
ATCGAAATTG
CCGTCAACCA
AGCTCTGATA
GAGTTGGTCA
AGACCAATGC
GGAGCATATA
CGCCCGGAGT
CGTGGCGATC
CTGCAAGCTC
CGGATGCCTC
CGCTCGAAGT
AGCGCGTCTG
CTGCTCCATA
CAAGCCAACC
ACGGCCTCCA
GAAGAAGATG
TTGGCGACCT
CGTATTGGGA
ATCCCCGAAC
ATCGCCTCGC
TCCAGTCAAT
GACCGCTGTT
ATGCGGCCAT
TGTCCGTCAG
GACATTGTTG
GAGCCGAAAT
CCGCGTGCAC
GAGGTGCCGG
ACTTCGGGGC
AGTCCTCGGC
CCAAAGCATC
AGCTCATCGA
GAGCCTGCGC
GACGGACGCA
CTGACGGTGT
CGTCCATCAC
AGTTTGCCAG
TGATACACAT
GGGGATCAGC
AATCGCGCAT
ATGAAATCAC
GCCATGTAGT
GTATTGACCG
ATTCCTTGCG
GTCCGAATGG
GCCGAACCCG
CTCGTCTGGC
TAAGATCGGC
CGCAGCGATC
GCATCCATAG
CCTCCGCGAC
CGGTTGTAGA
ACAGCGGGCA
GTTCGGTTTC
AGGCAGGTCT
TGCAACGTGA
CACCCTGTGA
ACGGCGGGAG
ATGCAATAGG
TCAGGCTCTC
GCTAAACTCC
CCAATGTCAA
GCACTTCCGG
AATCGGGAGC
GCGGCCGATG
CAAAGTGCCG
ATAAACATAA
CGATCTTTGT
AGAAACCATC
GGCGCAGCTA
TTTACCCGCA
GGACATATCC
ACGCCCTCCT
ACATCGAAGC
TGAAAGCACG
AGATTCTTCG
CCCTCCGAGA
GCTGCATCAG
GTCGGAGACG
CTGTCGAACT
TTTCGATCAG
AAACTTCTCG
ACAGACGTCG
CGGTGAGTTC
AGGCTTTTTC
ATATCTCATT
GCCCCCCGGA
TCTGCGAAAG
CTCGAGAGAG
ATAGATTTGT
AGAGAGAGAC
TGGTGATTTC
AGCGTGTCCT
CTCCAAATGA
AATGAACTTC
CTTATATAGA
GGAAGGGTCT
TGCGAAGGAT
AGTGGGATTG
TGCGTCATCC
CTTACGTCAG
TGGAGATATC
ACATCAATCC
ACTTGCTTTG
AAGACGTGGT
TGGAACGTCT
TCTTTTTCCA
CGATGCTCCT
CGTGGGTGGG
GGTCCATCTT
TGGGACCACT
GTCGGCAGAG
GCATCTTGAA
CGATAGCCTT
TCCTTTATCG
CAATGATGGC
ATTTGTAGGT
GCCACCTTCC
TTTTCTACTG
TCCTTTTGAT
GAAGTGACAG
ATAGCTGGGC
AATGGAATCC
GAGGAGGTTT
CCCGATATTA
CCCTTTGTTG
AAAAGTCTCA
ATAGCCCTTT
GGTCTTCTGA
GACTGTATCT
TTGATATTCT
TGGAGTAGAC
GAGAGTGTCG
TGCTCCACCA
TGTTCACATC
AATCCACTTG
CTTTGAAGAC
GTGGTTGGAA
CGTCTTCTTT
TTCCACGATG
CTCCTCGTGG
GTGGGGGTCC
ATCTTTGGGA
CCACTGTCGG
CAGAGGCATC
TTGAACGATA
GCCTTTCCTT
TATCGCAATG
ATGGCATTTG
TAGGTGCCAC
CTTCCTTTTC
TACTGTCCTT
TTGATGAAGT
GACAGATAGC
TGGGCAATGG
AATCCGAGGA
GGTTTCCCGA
TATTACCCTT
TGTTGAAAAG
TCTCAATAGC
CCTTTGGTCT
TCTGAGACTG
TATCTTTGAT
ATTCTTGGAG
TAGACGAGAG
TGTCGTGCTC
CACCATGTTG
GCAAGCTGCT
CTAGCCAATA
CGCAAACCGC
CTCTCCCCGC
GCGTTGGCCG
ATTCATTAAT
GCAGCTGGCA
CGACAGGTTT
CCCGACTGGA
AAGCGGGCAG
TGAGCGCAAC
GCAATTAATG
TGAGTTAGCT
CACTCATTAG
GCACCCCAGG
CTTTACACTT
TATGCTTCCG
GCTCGTATGT
TGTGTGGAAT
TGTGAGCGGA
TAACAATTTC
ACACAGGAAA
CAGCTATGAC
ATGATTACGA
ATTCGAGCTC
GGTACCCGGG
GATCGGCGCG
CCAGATTTGC
CTTTTCAATT
TCAGAAAGAA
TGCTAACCCA
CAGATGGTTA
GAGAGGCTTA
CGCAGCAGGT
ATCATCAAGA
CGATCTACCC
GAGCAATAAT
CTCCAGGAAA
TCAAATACCT
TCCCAAGAAG
GTTAAAGATG
CAGTCAAAAG
ATTCAGGACT
AACTGCATCA
AGAACACAGA
GAAAGATATA
TTTCTCAAGA
TCAGAAGTAC
TATTCCAGTA
TGGACGATTC
AAGGCTTGCT
TCACAAACCA
AGGCAAGTAA
TAGAGATTGG
AGTCTCTAAA
AAGGTAGTTC
CCACTGAATC
AAAGGCCATG
GAGTCAAAGA
TTCAAATAGA
GGACCTAACA
GAACTCGCCG
TAAAGACTGG
CGAACAGTTC
ATACAGAGTC
TCTTACGACT
CAATGACAAG
AAGAAAATCT
TCGTCAACAT
GGTGGAGCAC
GACACACTTG
TCTACTCCAA
AAATATCAAA
GATACAGTCT
CAGAAGACCA
AAGGGCAATT
GAGACTTTTC
AACAAAGGGT
AATATCCGGA
AACCTCCTCG
GATTCCATTG
CCCAGCTATC
TGTCACTTTA
TTGTGAAGAT
AGTGGAAAAG
GAAGGTGGCT
CCTACAAATG
CCATCATTGC
GATAAAGGAA
AGGCCATCGT
TGAAGATGCC
TCTGCCGACA
GTGGTCCCAA
AGATGGACCC
CCACCCACGA
GGAGCATCGT
GGAAAAAGAA
GACGTTCCAA
CCACGTCTTC
AAAGCAAGTG
GATTGATGTG
ATATCTCCAC
TGACGTAAGG
GATGACGCAC
AATCCCACTA
TCCTTCGCAA
GACCCTTCCT
CTATATAAGG
AAGTTCATTT
CATTTGGAGA
GAACACGGGG
GACTCCTGCA
GGTAGATCGC
TCGTCGACAT
GGATAAGAAG
TACTCTATCG
GACTCGCTAT
CGGAACTAAC
TCTGTGGGAT
GGGCTGTGAT
CACCGATGAG
TACAAGGTGC
CATCTAAGAA
GTTCAAGGTT
CTCGGAAACA
CCGATAGGCA
CTCTATCAAG
AAAAACCTTA
TCGGTGCTCT
CCTCTTCGAT
TCTGGTGAAA
CTGCTGAGGC
TACCAGACTC
AAGAGAACCG
CTAGAAGAAG
GTACACCAGA
AGAAAGAACA
GGATCTGCTA
CCTCCAAGAG
ATCTTCTCTA
ACGAGATGGC
TAAAGTGGAT
GATTCATTCT
TCCACAGGCT
CGAAGAGTCA
TTCCTCGTGG
AAGAAGATAA
GAAGCACGAG
AGGCACCCTA
TCTTCGGAAA
CATCGTTGAT
GAGGTGGCAT
ACCACGAGAA
GTACCCTACT
ATCTACCACC
TCAGAAAGAA
GCTCGTTGAT
TCTACTGATA
AGGCTGATCT
CAGGCTCATC
TACCTCGCTC
TCGCTCACAT
GATCAAGTTC
AGAGGACACT
TCCTCATCGA
GGGTGATCTC
AACCCTGATA
ACTCTGATGT
GGATAAGTTG
TTCATCCAGC
TCGTGCAGAC
CTACAACCAG
CTTTTCGAAG
AGAACCCTAT
CAACGCTTCA
GGTGTGGATG
CTAAGGCTAT
CCTCTCTGCT
AGGCTCTCTA
AGTCAAGAAG
GCTTGAGAAC
CTCATTGCTC
AGCTCCCTGG
TGAGAAGAAG
AACGGACTTT
TCGGAAACTT
GATCGCTCTC
TCTCTCGGAC
TCACCCCTAA
CTTCAAGTCT
AACTTCGATC
TCGCTGAGGA
TGCAAAGCTC
CAGCTCTCAA
AGGATACCTA
CGATGATGAT
CTCGATAACC
TCCTCGCTCA
GATCGGAGAT
CAGTACGCTG
ATTTGTTCCT
CGCTGCTAAG
AACCTCTCTG
ATGCTATCCT
CCTCAGTGAT
ATCCTCAGAG
TGAACACCGA
GATCACCAAG
GCTCCACTCT
CAGCTTCTAT
GATCAAGAGA
TACGATGAGC
ACCACCAGGA
TCTCACACTT
CTCAAGGCTC
TTGTTAGACA
GCAGCTCCCA
GAGAAGTACA
AAGAGATTTT
CTTCGATCAG
TCTAAGAACG
GATACGCTGG
TTACATCGAT
GGTGGTGCAT
CTCAAGAAGA
GTTCTACAAG
TTCATCAAGC
CTATCCTCGA
GAAGATGGAT
GGAACCGAGG
AACTCCTCGT
GAAGCTCAAT
AGAGAGGATC
TTCTCAGAAA
GCAGAGGACC
TTCGATAACG
GATCTATCCC
TCATCAGATC
CACCTCGGAG
AGTTGCACGC
TATCCTTAGA
AGGCAAGAGG
ATTTCTACCC
ATTCCTCAAG
GATAACAGGG
AAAAGATTGA
GAAGATTCTC
ACCTTCAGAA
TCCCTTACTA
CGTGGGACCT
CTCGCTAGAG
GAAACTCAAG
ATTCGCTTGG
ATGACCAGAA
AGTCTGAGGA
AACCATCACC
CCTTGGAACT
TCGAAGAGGT
GGTGGATAAG
GGTGCTAGTG
CTCAGTCTTT
CATCGAGAGG
ATGACCAACT
TCGATAAGAA
CCTTCCAAAC
GAGAAGGTGC
TCCCTAAGCA
CTCTTTGCTC
TACGAGTACT
TCACCGTGTA
CAACGAGTTG
ACCAAGGTTA
AGTACGTGAC
CGAGGGAATG
AGGAAGCCTG
CTTTTTTGTC
AGGTGAGCAA
AAGAAGGCTA
TCGTTGATCT
CTTGTTCAAG
ACCAACAGAA
AGGTGACCGT
GAAGCAGCTC
AAAGAGGATT
ACTTCAAGAA
AATCGAGTGC
TTCGATTCAG
TTGAGATTTC
TGGTGTTGAG
GATAGGTTCA
ACGCATCTCT
CGGAACCTAC
CACGATCTCC
TCAAGATCAT
TAAGGATAAG
GATTTCTTGG
ATAACGAGGA
AAACGAGGAT
ATCTTGGAGG
ATATCGTTCT
TACCCTCACC
CTCTTTGAAG
ATAGAGAGAT
GATTGAAGAA
AGGCTCAAGA
CCTACGCTCA
TCTCTTCGAT
GATAAGGTGA
TGAAGCAGTT
GAAGAGAAGA
AGATACACTG
GTTGGGGAAG
GCTCTCAAGA
AAGCTCATTA
ACGGAATCAG
GGATAAGCAG
TCTGGAAAGA
CAATCCTTGA
TTTCCTCAAG
TCTGATGGAT
TCGCTAACAG
AAACTTCATG
CAGCTCATCC
ACGATGATTC
TCTCACCTTT
AAAGAGGATA
TCCAGAAGGC
TCAGGTTTCA
GGACAGGGTG
ATAGTCTCCA
TGAGCATATC
GCTAACCTCG
CTGGATCTCC
TGCAATCAAG
AAGGGAATCC
TCCAGACTGT
GAAGGTTGTG
GATGAGTTGG
TGAAGGTGAT
GGGAAGGCAT
AAGCCTGAGA
ACATCGTGAT
CGAAATGGCT
AGAGAGAACC
AGACCACTCA
GAAGGGACAG
AAGAACTCTA
GGGAAAGGAT
GAAGAGGATC
GAGGAAGGTA
TCAAAGAGCT
TGGATCTCAG
ATCCTCAAAG
AGCACCCTGT
TGAGAACACT
CAGCTCCAGA
ATGAGAAGCT
CTACCTCTAC
TACCTCCAGA
ACGGAAGGGA
TATGTATGTG
GATCAAGAGT
TGGATATCAA
CAGGCTCTCT
GATTACGATG
TTGATGCTAT
CGTGCCACAG
TCATTCTTGA
AGGATGATTC
TATCGATAAC
AAGGTGCTCA
CCAGGTCTGA
TAAGAACAGG
GGTAAGAGTG
ATAACGTGCC
AAGTGAAGAG
GTTGTGAAGA
AAATGAAGAA
CTATTGGAGG
CAGCTCCTCA
ACGCTAAGCT
CATCACTCAG
AGAAAGTTCG
ATAACTTGAC
TAAGGCTGAG
AGGGGAGGAC
TCTCTGAATT
GGATAAGGCA
GGATTCATCA
AGAGGCAGCT
TGTGGAAACC
AGGCAGATCA
CTAAGCACGT
TGCACAGATC
CTCGATTCTA
GGATGAACAC
CAAGTACGAT
GAGAACGATA
AGTTGATCAG
GGAAGTGAAG
GTTATCACCC
TCAAGTCAAA
GCTCGTGTCT
GATTTCAGAA
AGGATTTCCA
ATTCTACAAG
GTGAGGGAAA
TCAACAACTA
CCACCACGCT
CACGATGCTT
ACCTTAACGC
TGTTGTTGGA
ACCGCTCTCA
TCAAGAAGTA
TCCTAAGCTC
GAGTCAGAGT
TCGTGTACGG
TGATTACAAG
GTGTACGATG
TGAGGAAGAT
GATCGCTAAG
TCTGAGCAAG
AGATCGGAAA
GGCTACCGCT
AAGTATTTCT
TCTACTCTAA
CATCATGAAT
TTCTTCAAGA
CCGAGATTAC
CCTCGCTAAC
GGTGAGATCA
GAAAGAGGCC
ACTCATCGAG
ACAAACGGTG
AAACAGGTGA
GATCGTGTGG
GATAAGGGAA
GGGATTTCGC
TACCGTTAGA
AAGGTGCTCT
CTATGCCACA
GGTGAACATC
GTTAAGAAAA
CCGAGGTGCA
GACCGGTGGA
TTCTCTAAAG
AGTCTATCCT
CCCTAAGAGG
AACTCTGATA
AGCTCATTGC
TAGGAAGAAG
GATTGGGACC
CTAAGAAATA
CGGTGGTTTC
GATTCTCCTA
CCGTGGCTTA
CTCTGTTCTC
GTTGTGGCTA
AGGTTGAGAA
GGGAAAGAGT
AAGAAGCTCA
AGTCTGTTAA
GGAACTTCTC
GGAATCACTA
TCATGGAAAG
GTCATCTTTC
GAGAAGAACC
CAATCGATTT
CCTCGAGGCT
AAGGGATACA
AAGAGGTTAA
GAAGGATCTC
ATCATCAAGC
TCCCAAAGTA
CTCACTCTTC
GAACTCGAGA
ACGGTAGAAA
GAGGATGCTC
GCTTCTGCTG
GTGAGCTTCA
AAAGGGAAAC
GAGCTTGCTC
TCCCATCTAA
GTACGTTAAC
TTTCTTTACC
TCGCTTCTCA
CTACGAGAAG
TTGAAGGGAT
CTCCAGAAGA
TAACGAGCAG
AAGCAACTTT
TCGTTGAGCA
GCACAAGCAC
TACTTGGATG
AGATCATCGA
GCAGATCTCT
GAGTTCTCTA
AAAGGGTGAT
CCTCGCTGAT
GCAAACCTCG
ATAAGGTGTT
GTCTGCTTAC
AACAAGCACA
GAGATAAGCC
TATCAGGGAA
CAGGCAGAGA
ACATCATCCA
TCTCTTCACC
CTTACCAACC
TCGGTGCTCC
TGCTGCTTTC
AAGTACTTCG
ATACAACCAT
CGATAGGAAG
AGATACACCT
CTACCAAAGA
AGTGCTCGAT
GCTACCCTCA
TCCATCAGTC
TATCACTGGA
CTCTACGAGA
CTAGGATCGA
TCTCTCACAG
CTCGGTGGTG
ATTCAAGGGC
TGATCCTAAG
AAGAAGAGGA
AGGTTTGACT
CGAGATATGA
AGATGAAGAT
GAAATATTTG
GTGTGTCAAA
TAAAAAGCTT
GTGTGCTTAA
GTTTGTGTTT
TTTTCTTGGC
TTGTTGTGTT
ATGAATTTGT
GGCTTTTTCT
AATATTAAAT
GAATGTAAGA
TCACATTATA
ATGAATAAAC
AAATGTTTCT
ATAATCCATT
GTGAATGTTT
TGTTGGATCT
CTTCTGCAGC
ATATAACTAC
TGTATGTGCT
ATGGTATGGA
CTATGGAATA
TGATTAAAGA
TAAGGAGCTC
CGGTGACGGA
CTGGCACTGG
CCGTCGTTTT
ACAACGTCGT
GACTGGGAAA
ACCCTGGCGT
TACCCAACTT
AATCGCCTTG
CAGCACATCC
CCCTTTCGCC
AGCTGGCGTA
ATAGCGAAGA
GGCCCGCACC
GATCGCCCTT
CCCAACAGTT
GCGCAGCCTG
AATGGCGAAT
GCTAGAGCAG
CTTGAGCTTG
GATCAGATTG
TCGTTTCCCG
CCTTCAGTTT
AAACTATCAG
TGTTTGACAG
GATATATTGG
CGGGTAAACC
TAAGAGAAAA
GAGCGTTTAT
TAGAATAATC
GGATATTTAA
AAGGGCGTGA
AAAGGTTTAT
CCGTTCGTCC
ATTTGTATGT
GCATGCCAAC
CACAGGGTTC
CCCTCGGGAT
CAAAGTACTT
TGATCCAACC
CCTCCGCTGC
TATAGTGCAG
TCGGCTTCTG
ACGTTCAGTG
CAGCCGTCTT
CTGAAAACGA
CATGTCGCAC
AAGTCCTAAG
TTACGCGACA
GGCTGCCGCC
CTGCCCTTTT
CCTGGCGTTT
TCTTGTCGCG
TGTTTTAGTC
GCATAAAGTA
GAATACTTGC
GACTAGAACC
GGAGACATTA
CGCCATGAAC
AAGAGCGCCG
CCGCTGGCCT
GCTGGGCTAT
GCCCGCGTCA
GCACCGACGA
CCAGGACTTG
ACCAACCAAC
GGGCCGAACT
GCACGCGGCC
GGCTGCACCA
AGCTGTTTTC
CGAGAAGATC
ACCGGCACCA
GGCGCGACCG
CCCGGAGCTG
GCCAGGATGC
TTGACCACCT
ACGCCCTGGC
GACGTTGTGA
CAGTGACCAG
GCTAGACCGC
CTGGCCCGCA
GCACCCGCGA
CCTACTGGAC
ATTGCCGAGC
GCATCCAGGA
GGCCGGCGCG
GGCCTGCGTA
GCCTGGCAGA
GCCGTGGGCC
GACACCACCA
CGCCGGCCGG
CCGCATGGTG
TTGACCGTGT
TCGCCGGCAT
TGCCGAGTTC
GAGCGTTCCC
TAATCATCGA
CCGCACCCGG
AGCGGGCGCG
AGGCCGCCAA
GGCCCGAGGC
GTGAAGTTTG
GCCCCCGCCC
TACCCTCACC
CCGGCACAGA
TCGCGCACGC
CCGCGAGCTG
ATCGACCAGG
AAGGCCGCAC
CGTGAAAGAG
GCGGCTGCAC
TGCTTGGCGT
GCATCGCTCG
ACCCTGTACC
GCGCACTTGA
GCGCAGCGAG
GAAGTGACGC
CCACCGAGGC
CAGGCGGCGC
GGTGCCTTCC
GTGAGGACGC
ATTGACCGAG
GCCGACGCCC
TGGCGGCCGC
CGAGAATGAA
CGCCAAGAGG
AACAAGCATG
AAACCGCACC
AGGACGGCCA
GGACGAACCG
TTTTTCATTA
CCGAAGAGAT
CGAGGCGGAG
ATGATCGCGG
CCGGGTACGT
GTTCGAGCCG
CCCGCGCACG
TCTCAACCGT
GCGGCTGCAT
GAAATCCTGG
CCGGTTTGTC
TGATGCCAAG
CTGGCGGCCT
GGCCGGCCAG
CTTGGCCGCT
GAAGAAACCG
AGCGCCGCCG
TCTAAAAAGG
TGATGTGTAT
TTGAGTAAAA
CAGCTTGCGT
CATGCGGTCG
CTGCGTATAT
GATGCGATGA
GTAAATAAAC
AAATACGCAA
GGGGAACGCA
TGAAGGTTAT
CGCTGTACTT
AACCAGAAAG
GCGGGTCAGG
CAAGACGACC
ATCGCAACCC
ATCTAGCCCG
CGCCCTGCAA
CTCGCCGGGG
CCGATGTTCT
GTTAGTCGAT
TCCGATCCCC
AGGGCAGTGC
CCGCGATTGG
GCGGCCGTGC
GGGAAGATCA
ACCGCTAACC
GTTGTCGGCA
TCGACCGCCC
GACGATTGAC
CGCGACGTGA
AGGCCATCGG
CCGGCGCGAC
TTCGTAGTGA
TCGACGGAGC
GCCCCAGGCG
GCGGACTTGG
CTGTGTCCGC
GATCAAGGCA
GCCGACTTCG
TGCTGATTCC
GGTGCAGCCA
AGCCCTTACG
ACATATGGGC
CACCGCCGAC
CTGGTGGAGC
TGGTTAAGCA
GCGCATTGAG
GTCACGGATG
GAAGGCTACA
AGCGGCCTTT
GTCGTGTCGC
GGGCGATCAA
AGGCACGCGC
ATCGGCGGTG
AGGTTGCCGA
GGCGCTGGCC
GGGTACGAGC
TGCCCATTCT
TGAGTCCCGT
ATCACGCAGC
GCGTGAGCTA
CCCAGGCACT
GCCGCCGCCG
GCACAACCGT
TCTTGAATCA
GAACCCGAGG
GCGACGCTGC
CCGCGAGGTC
CAGGCGCTGG
CCGCTGAAAT
TAAATCAAAA
CTCATTTGAG
TTAATGAGGT
AAAGAGAAAA
TGAGCAAAAG
CACAAACACG
CTAAGTGCCG
GCCGTCCGAG
CGCACGCAGC
AGCAAGGCTG
CAACGTTGGC
CAGCCTGGCA
GACACGCCAG
CCATGAAGCG
GGTCAACTTT
CAGTTGCCGG
CGGAGGATCA
CACCAAGCTG
AAGATGTACG
CGGTACGCCA
AGGCAAGACC
ATTACCGAGC
TGCTATCTGA
ATACATCGCG
CAGCTACCAG
AGTAAATGAG
CAAATGAATA
AATGAGTAGA
TGAATTTTAG
CGGCTAAAGG
AGGCGGCATG
GAAAATCAAG
AACAACCAGG
CACCGACGCC
GTGGAATGCC
CCATGTGTGG
AGGAACGGGC
GGTTGGCCAG
GCGTAAGCGG
CTGGGTTGCC
TGCCGGCCCT
GCAATGGCAC
TGGAACCCCC
AAGCCCGAGG
AATCGGCGTG
AGCGGTCGCA
AACCATCCGG
CCCGGTACAA
ATCGGCGCGG
CGCTGGGTGA
TGACCTGGTG
GAGAAGTTGA
AGGCCGCGCA
GGCCGCCCAG
CGGCAACGCA
TCGAGGCAGA
AGCACGCCCC
GGTGAATCGT
GGCAAGCGGC
CGCTGATCGA
ATCCGCAAAG
AATCCCGGCA
ACCGCCGGCA
GCCGGTGCGC
CGTCGATTAG
GAAGCCGCCC
AAGGGCGACG
AGCAACCAGA
TTTTTTCGTT
CCGATGCTCT
ATGACGTGGG
CACCCGCGAT
AGTCGCAGCA
TCATGGACGT
GGCCGTTTTC
CGTCTGTCGA
AGCGTGACCG
ACGAGCTGGC
GAGGTGATCC
GCTACGAGCT
TCCAGACGGG
CACGTAGAGG
TTTCCGCAGG
GCCGGCCGGC
ATGGCCAGTG
TGTGGGATTA
CGACCTGGTA
CTGATGGCGG
TTTCCCATCT
AACCGAATCC
ATGAACCGAT
ACCGGGAAGG
GAAGGGAGAC
AAGCCCGGCC
GCGTGTTCCG
TCCACACGTT
GCGGACGTAC
TCAAGTTCTG
CCGGCGAGCC
GATGGCGGAA
AGCAGAAAGA
CGACCTGGTA
GAAACCTGCA
TTCGGTTAAA
CACCACGCAC
GTTGCCATGC
AGCGTACGAA
GAAGGCCAAG
AACGGCCGCC
TGGTGACGGT
ATCCGAGGGT
GAAGCCTTGA
TTAGCCGCTA
CAAGATCGTA
AAGAGCGAAA
CCGGGCGGCC
GGAGTACATC
GAGATCGAGC
TAGCTGATTG
GATGTACCGC
GAGATCACAG
AAGGCAAGAA
CCCGGACGTG
CTGACGGTTC
ACCCCGATTA
CTTTTTGATC
GATCCCGGCA
TCGGCCGTTT
TCTCTACCGC
CTGGCACGCC
GCGCCGCAGG
CAAGGCAGAA
GCCAGATGGT
TGTTCAAGAC
GATCTACGAA
CGCAGTGGCA
GCGCCGGAGA
GTTCAAGAAG
TTCTGTTTCA
CCGTGCGCAA
GCTGATCGGG
TCAAATGACC
TGCCGGAGTA
CGATTTGAAG
GAGGAGGCGG
GGCAGGCTGG
CCCGATCCTA
GTCATGCGCT
ACCGCAACCT
GATCGAGGGC
GAAGCATCCG
CCGGTTCCTA
ATGTACGGAG
CAGATGCTAG
GGCAAATTGC
CCTAGCAGGG
GAAAAAGGTC
GAAAAGGTCT
CTTTCCTGTG
GATAGCACGT
ACATTGGGAA
CCCAAAGCCG
TACATTGGGA
ACCGGAACCC
GTACATTGGG
AACCCAAAGC
CGTACATTGG
GAACCGGTCA
CACATGTAAG
TGACTGATAT
AAAAGAGAAA
AAAGGCGATT
TTTCCGCCTA
AAACTCTTTA
AAACTTATTA
AAACTCTTAA
AACCCGCCTG
GCCTGTGCAT
AACTGTCTGG
CCAGCGCACA
GCCGAAGAGC
TGCAAAAAGC
GCCTACCCTT
CGGTCGCTGC
GCTCCCTACG
CCCCGCCGCT
TCGCGTCGGC
CTATCGCGGC
CGCTGGCCGC
TCAAAAATGG
CTGGCCTACG
GCCAGGCAAT
CTACCAGGGC
GCGGACAAGC
CGCGCCGTCG
CCACTCGACC
GCCGGCGCCC
ACATCAAGGC
ACCCTGCCTC
GCGCGTTTCG
GTGATGACGG
TGAAAACCTC
TGACACATGC
AGCTCCCGGA
GACGGTCACA
GCTTGTCTGT
AAGCGGATGC
CGGGAGCAGA
CAAGCCCGTC
AGGGCGCGTC
AGCGGGTGTT
GGCGGGTGTC
GGGGCGCAGC
CATGACCCAG
TCACGTAGCG
ATAGCGGAGT
GTATACTGGC
TTAACTATGC
GGCATCAGAG
CAGATTGTAC
TGAGAGTGCA
CCATATGCGG
TGTGAAATAC
CGCACAGATG
CGTAAGGAGA
AAATACCGCA
TCAGGCGCTC
TTCCGCTTCC
TCGCTCACTG
ACTCGCTGCG
CTCGGTCGTT
CGGCTGCGGC
GAGCGGTATC
AGCTCACTCA
AAGGCGGTAA
TACGGTTATC
CACAGAATCA
GGGGATAACG
CAGGAAAGAA
CATGTGAGCA
AAAGGCCAGC
AAAAGGCCAG
GAACCGTAAA
AAGGCCGCGT
TGCTGGCGTT
TTTCCATAGG
CTCCGCCCCC
CTGACGAGCA
TCACAAAAAT
CGACGCTCAA
GTCAGAGGTG
GCGAAACCCG
ACAGGACTAT
AAAGATACCA
GGCGTTTCCC
CCTGGAAGCT
CCCTCGTGCG
CTCTCCTGTT
CCGACCCTGC
CGCTTACCGG
ATACCTGTCC
GCCTTTCTCC
CTTCGGGAAG
CGTGGCGCTT
TCTCATAGCT
CACGCTGTAG
GTATCTCAGT
TCGGTGTAGG
TCGTTCGCTC
CAAGCTGGGC
TGTGTGCACG
AACCCCCCGT
TCAGCCCGAC
CGCTGCGCCT
TATCCGGTAA
CTATCGTCTT
GAGTCCAACC
CGGTAAGACA
CGACTTATCG
CCACTGGCAG
CAGCCACTGG
TAACAGGATT
AGCAGAGCGA
GGTAT