名称: | pBI121-EGFP-Vac |
---|---|
别称: | 液泡定位 |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 亚细胞定位 |
片段类型: | ORF |
片段物种: | |
原核抗性: | Kan |
筛选标记: | Neo/G418 |
荧光标记: | 绿色 |
启动子: | 35s |
复制子: | oriV |
拷贝数: | 低 |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGAGCGTCGC
AAAGGCGCTC
GGTCTTGCCT
TGCTCGTCGG
TGATGTACTT
CACCAGCTCC
GCGAAGTCGC
TCTTCTTGAT
GGAGCGCATG
GGGACGTGCT
TGGCAATCAC
GCGCACCCCC
CGGCCGTTTT
AGCGGCTAAA
AAAGTCATGG
CTCTGCCCTC
GGGCGGACCA
CGCCCATCAT
GACCTTGCCA
AGCTCGTCCT
GCTTCTCTTC
GATCTTCGCC
AGCAGGGCGA
GGATCGTGGC
ATCACCGAAC
CGCGCCGTGC
GCGGGTCGTC
GGTGAGCCAG
AGTTTCAGCA
GGCCGCCCAG
GCGGCCCAGG
TCGCCATTGA
TGCGGGCCAG
CTCGCGGACG
TGCTCATAGT
CCACGACGCC
CGTGATTTTG
TAGCCCTGGC
CGACGGCCAG
CAGGTAGGCC
GACAGGCTCA
TGCCGGCCGC
CGCCGCCTTT
TCCTCAATCG
CTCTTCGTTC
GTCTGGAAGG
CAGTACACCT
TGATAGGTGG
GCTGCCCTTC
CTGGTTGGCT
TGGTTTCATC
AGCCATCCGC
TTGCCCTCAT
CTGTTACGCC
GGCGGTAGCC
GGCCAGCCTC
GCAGAGCAGG
ATTCCCGTTG
AGCACCGCCA
GGTGCGAATA
AGGGACAGTG
AAGAAGGAAC
ACCCGCTCGC
GGGTGGGCCT
ACTTCACCTA
TCCTGCCCGG
CTGACGCCGT
TGGATACACC
AAGGAAAGTC
TACACGAACC
CTTTGGCAAA
ATCCTGTATA
TCGTGCGAAA
AAGGATGGAT
ATACCGAAAA
AATCGCTATA
ATGACCCCGA
AGCAGGGTTA
TGCAGCGGAA
AAGCGCCACG
CTTCCCGAAG
GGAGAAAGGC
GGACAGGTAT
CCGGTAAGCG
GCAGGGTCGG
AACAGGAGAG
CGCACGAGGG
AGCTTCCAGG
GGGAAACGCC
TGGTATCTTT
ATAGTCCTGT
CGGGTTTCGC
CACCTCTGAC
TTGAGCGTCG
ATTTTTGTGA
TGCTCGTCAG
GGGGGCGGAG
CCTATGGAAA
AACGCCAGCA
ACGCGGCCTT
TTTACGGTTC
CTGGCCTTTT
GCTGGCCTTT
TGCTCACATG
TTCTTTCCTG
CGTTATCCCC
TGATTCTGTG
GATAACCGTA
TTACCGCCTT
TGAGTGAGCT
GATACCGCTC
GCCGCAGCCG
AACGACCGAG
CGCAGCGAGT
CAGTGAGCGA
GGAAGCGGAA
GAGCGCCAGA
AGGCCGCCAG
AGAGGCCGAG
CGCGGCCGTG
AGGCTTGGAC
GCTAGGGCAG
GGCATGAAAA
AGCCCGTAGC
GGGCTGCTAC
GGGCGTCTGA
CGCGGTGGAA
AGGGGGAGGG
GATGTTGTCT
ACATGGCTCT
GCTGTAGTGA
GTGGGTTGCG
CTCCGGCAGC
GGTCCTGATC
AATCGTCACC
CTTTCTCGGT
CCTTCAACGT
TCCTGACAAC
GAGCCTCCTT
TTCGCCAATC
CATCGACAAT
CACCGCGAGT
CCCTGCTCGA
ACGCTGCGTC
CGGACCGGCT
TCGTCGAAGG
CGTCTATCGC
GGCCCGCAAC
AGCGGCGAGA
GCGGAGCCTG
TTCAACGGTG
CCGCCGCGCT
CGCCGGCATC
GCTGTCGCCG
GCCTGCTCCT
CAAGCACGGC
CCCAACAGTG
AAGTAGCTGA
TTGTCATCAG
CGCATTGACG
GCGTCCCCGG
CCGAAAAACC
CGCCTCGCAG
AGGAAGCGAA
GCTGCGCGTC
GGCCGTTTCC
ATCTGCGGTG
CGCCCGGTCG
CGTGCCGGCA
TGGATGCGCG
CGCCATCGCG
GTAGGCGAGC
AGCGCCTGCC
TGAAGCTGCG
GGCATTCCCG
ATCAGAAATG
AGCGCCAGTC
GTCGTCGGCT
CTCGGCACCG
AATGCGTATG
ATTCTCCGCC
AGCATGGCTT
CGGCCAGTGC
GTCGAGCAGC
GCCCGCTTGT
TCCTGAAGTG
CCAGTAAAGC
GCCGGCTGCT
GAACCCCCAA
CCGTTCCGCC
AGTTTGCGTG
TCGTCAGACC
GTCTACGCCG
ACCTCGTTCA
ACAGGTCCAG
GGCGGCACGG
ATCACTGTAT
TCGGCTGCAA
CTTTGTCATG
CTTGACACTT
TATCACTGAT
AAACATAATA
TGTCCACCAA
CTTATCAGTG
ATAAAGAATC
CGCGCGTTCA
ATCGGACCAG
CGGAGGCTGG
TCCGGAGGCC
AGACGTGAAA
CCCAACATAC
CCCTGATCGT
AATTCTGAGC
ACTGTCGCGC
TCGACGCTGT
CGGCATCGGC
CTGATTATGC
CGGTGCTGCC
GGGCCTCCTG
CGCGATCTGG
TTCACTCGAA
CGACGTCACC
GCCCACTATG
GCATTCTGCT
GGCGCTGTAT
GCGTTGGTGC
AATTTGCCTG
CGCACCTGTG
CTGGGCGCGC
TGTCGGATCG
TTTCGGGCGG
CGGCCAATCT
TGCTCGTCTC
GCTGGCCGGC
GCCAGATCTG
GGGAACCCTG
TGGTTGGCAT
GCACATACAA
ATGGACGAAC
GGATAAACCT
TTTCACGCCC
TTTTAAATAT
CCGATTATTC
TAATAAACGC
TCTTTTCTCT
TAGGTTTACC
CGCCAATATA
TCCTGTCAAA
CACTGATAGT
TTAAACTGAA
GGCGGGAAAC
GACAATCTGA
TCATGAGCGG
AGAATTAAGG
GAGTCACGTT
ATGACCCCCG
CCGATGACGC
GGGACAAGCC
GTTTTACGTT
TGGAACTGAC
AGAACCGCAA
CGTTGAAGGA
GCCACTCAGC
CGCGGGTTTC
TGGAGTTTAA
TGAGCTAAGC
ACATACGTCA
GAAACCATTA
TTGCGCGTTC
AAAAGTCGCC
TAAGGTCACT
ATCAGCTAGC
AAATATTTCT
TGTCAAAAAT
GCTCCACTGA
CGTTCCATAA
ATTCCCCTCG
GTATCCAATT
AGAGTCTCAT
ATTCACTCTC
AATCCAAATA
ATCTGCACCG
GATCTGGATC
GTTTCGCATG
ATTGAACAAG
ATGGATTGCA
CGCAGGTTCT
CCGGCCGCTT
GGGTGGAGAG
GCTATTCGGC
TATGACTGGG
CACAACAGAC
AATCGGCTGC
TCTGATGCCG
CCGTGTTCCG
GCTGTCAGCG
CAGGGGCGCC
CGGTTCTTTT
TGTCAAGACC
GACCTGTCCG
GTGCCCTGAA
TGAACTGCAG
GACGAGGCAG
CGCGGCTATC
GTGGCTGGCC
ACGACGGGCG
TTCCTTGCGC
AGCTGTGCTC
GACGTTGTCA
CTGAAGCGGG
AAGGGACTGG
CTGCTATTGG
GCGAAGTGCC
GGGGCAGGAT
CTCCTGTCAT
CTCACCTTGC
TCCTGCCGAG
AAAGTATCCA
TCATGGCTGA
TGCAATGCGG
CGGCTGCATA
CGCTTGATCC
GGCTACCTGC
CCATTCGACC
ACCAAGCGAA
ACATCGCATC
GAGCGAGCAC
GTACTCGGAT
GGAAGCCGGT
CTTGTCGATC
AGGATGATCT
GGACGAAGAG
CATCAGGGGC
TCGCGCCAGC
CGAACTGTTC
GCCAGGCTCA
AGGCGCGCAT
GCCCGACGGC
GATGATCTCG
TCGTGACCCA
TGGCGATGCC
TGCTTGCCGA
ATATCATGGT
GGAAAATGGC
CGCTTTTCTG
GATTCATCGA
CTGTGGCCGG
CTGGGTGTGG
CGGACCGCTA
TCAGGACATA
GCGTTGGCTA
CCCGTGATAT
TGCTGAAGAG
CTTGGCGGCG
AATGGGCTGA
CCGCTTCCTC
GTGCTTTACG
GTATCGCCGC
TCCCGATTCG
CAGCGCATCG
CCTTCTATCG
CCTTCTTGAC
GAGTTCTTCT
GAGCGGGACT
CTGGGGTTCG
AAATGACCGA
CCAAGCGACG
CCCAACCTGC
CATCACGAGA
TTTCGATTCC
ACCGCCGCCT
TCTATGAAAG
GTTGGGCTTC
GGAATCGTTT
TCCGGGACGC
CGGCTGGATG
ATCCTCCAGC
GCGGGGATCT
CATGCTGGAG
TTCTTCGCCC
ACGGGATCTC
TGCGGAACAG
GCGGTCGAAG
GTGCCGATAT
CATTACGACA
GCAACGGCCG
ACAAGCACAA
CGCCACGATC
CTGAGCGACA
ATATGATCGG
GCCCGGCGTC
CACATCAACG
GCGTCGGCGG
CGACTGCCCA
GGCAAGACCG
AGATGCACCG
CGATATCTTG
CTGCGTTCGG
ATATTTTCGT
GGAGTTCCCG
CCACAGACCC
GGATGATCCC
CGATCGTTCA
AACATTTGGC
AATAAAGTTT
CTTAAGATTG
AATCCTGTTG
CCGGTCTTGC
GATGATTATC
ATATAATTTC
TGTTGAATTA
CGTTAAGCAT
GTAATAATTA
ACATGTAATG
CATGACGTTA
TTTATGAGAT
GGGTTTTTAT
GATTAGAGTC
CCGCAATTAT
ACATTTAATA
CGCGATAGAA
AACAAAATAT
AGCGCGCAAA
CTAGGATAAA
TTATCGCGCG
CGGTGTCATC
TATGTTACTA
GATCGGGCCT
CCTGTCAATG
CTGGCGGCGG
CTCTGGTGGT
GGTTCTGGTG
GCGGCTCTGA
GGGTGGTGGC
TCTGAGGGTG
GCGGTTCTGA
GGGTGGCGGC
TCTGAGGGAG
GCGGTTCCGG
TGGTGGCTCT
GGTTCCGGTG
ATTTTGATTA
TGAAAAGATG
GCAAACGCTA
ATAAGGGGGC
TATGACCGAA
AATGCCGATG
AAAACGCGCT
ACAGTCTGAC
GCTAAAGGCA
AACTTGATTC
TGTCGCTACT
GATTACGGTG
CTGCTATCGA
TGGTTTCATT
GGTGACGTTT
CCGGCCTTGC
TAATGGTAAT
GGTGCTACTG
GTGATTTTGC
TGGCTCTAAT
TCCCAAATGG
CTCAAGTCGG
TGACGGTGAT
AATTCACCTT
TAATGAATAA
TTTCCGTCAA
TATTTACCTT
CCCTCCCTCA
ATCGGTTGAA
TGTCGCCCTT
TTGTCTTTGG
CCCAATACGC
AAACCGCCTC
TCCCCGCGCG
TTGGCCGATT
CATTAATGCA
GCTGGCACGA
CAGGTTTCCC
GACTGGAAAG
CGGGCAGTGA
GCGCAACGCA
ATTAATGTGA
GTTAGCTCAC
TCATTAGGCA
CCCCAGGCTT
TACACTTTAT
GCTTCCGGCT
CGTATGTTGT
GTGGAATTGT
GAGCGGATAA
CAATTTCACA
CAGGAAACAG
CTATGACCAT
GATTACGCCA
AGCTTGCATG
CCTGCAGGTC
CCCAGATTAG
CCTTTTCAAT
TTCAGAAAGA
ATGCTAACCC
ACAGATGGTT
AGAGAGGCTT
ACGCAGCAGG
TCTCATCAAG
ACGATCTACC
CGAGCAATAA
TCTCCAGGAA
ATCAAATACC
TTCCCAAGAA
GGTTAAAGAT
GCAGTCAAAA
GATTCAGGAC
TAACTGCATC
AAGAACACAG
AGAAAGATAT
ATTTCTCAAG
ATCAGAAGTA
CTATTCCAGT
ATGGACGATT
CAAGGCTTGC
TTCACAAACC
AAGGCAAGTA
ATAGAGATTG
GAGTCTCTAA
AAAGGTAGTT
CCCACTGAAT
CAAAGGCCAT
GGAGTCAAAG
ATTCAAATAG
AGGACCTAAC
AGAACTCGCC
GTAAAGACTG
GCGAACAGTT
CATACAGAGT
CTCTTACGAC
TCAATGACAA
GAAGAAAATC
TTCGTCAACA
TGGTGGAGCA
CGACACACTT
GTCTACTCCA
AAAATATCAA
AGATACAGTC
TCAGAAGACC
AAAGGGCAAT
TGAGACTTTT
CAACAAAGGG
TAATATCCGG
AAACCTCCTC
GGATTCCATT
GCCCAGCTAT
CTGTCACTTT
ATTGTGAAGA
TAGTGGAAAA
GGAAGGTGGC
TCCTACAAAT
GCCATCATTG
CGATAAAGGA
AAGGCCATCG
TTGAAGATGC
CTCTGCCGAC
AGTGGTCCCA
AAGATGGACC
CCCACCCACG
AGGAGCATCG
TGGAAAAAGA
AGACGTTCCA
ACCACGTCTT
CAAAGCAAGT
GGATTGATGT
GATATCTCCA
CTGACGTAAG
GGATGACGCA
CAATCCCACT
ATCCTTCGCA
AGACCCTTCC
TCTATATAAG
GAAGTTCATT
TCATTTGGAG
AGAACACGGG
GGACTCTAGA
GGATCCCCGG
GGTACCATGG
TGAGCAAGGG
CGAGGAGCTG
TTCACCGGGG
TGGTGCCCAT
CCTGGTCGAG
CTGGACGGCG
ACGTAAACGG
CCACAAGTTC
AGCGTGTCCG
GCGAGGGCGA
GGGCGATGCC
ACCTACGGCA
AGCTGACCCT
GAAGTTCATC
TGCACCACCG
GCAAGCTGCC
CGTGCCCTGG
CCCACCCTCG
TGACCACCCT
GACCTACGGC
GTGCAGTGCT
TCAGCCGCTA
CCCCGACCAC
ATGAAGCAGC
ACGACTTCTT
CAAGTCCGCC
ATGCCCGAAG
GCTACGTCCA
GGAGCGCACC
ATCTTCTTCA
AGGACGACGG
CAACTACAAG
ACCCGCGCCG
AGGTGAAGTT
CGAGGGCGAC
ACCCTGGTGA
ACCGCATCGA
GCTGAAGGGC
ATCGACTTCA
AGGAGGACGG
CAACATCCTG
GGGCACAAGC
TGGAGTACAA
CTACAACAGC
CACAACGTCT
ATATCATGGC
CGACAAGCAG
AAGAACGGCA
TCAAGGTGAA
CTTCAAGATC
CGCCACAACA
TCGAGGACGG
CAGCGTGCAG
CTCGCCGACC
ACTACCAGCA
GAACACCCCC
ATCGGCGACG
GCCCCGTGCT
GCTGCCCGAC
AACCACTACC
TGAGCACCCA
GTCCGCCCTG
AGCAAAGACC
CCAACGAGAA
GCGCGATCAC
ATGGTCCTGC
TGGAGTTCGT
GACCGCCGCC
GGGATCACTC
TCGGCATGGA
CGAGCTGTAC
AAGCTCGAGA
TGGTGAATTC
GAATGAAGAA
GACGAAAGGA
GAATTCTTGA
CGTTGAAGAA
TCTCTTCTTA
CACAGGAGGA
AGTGAACAAT
CACTTGACCG
GATTGTCTTC
TACAGCTGAA
GATGGTTCTA
TTGACATTTA
TGGAAACCCA
CCATCGAAGA
AGAAGACAGG
AAACTGGAAA
GCTTGTCCCT
TTATTCTTGG
AAACGAATGT
TGTGAGAGAT
TAGCTTACTA
TGGAATTGCC
AAGAATCTAA
TCACTTACTA
CACAAGTGAA
TTACACGAGT
CCAATGTTTC
TGCAGCTAGT
GACGTTATGA
TTTGGCAAGG
AACTTGCTAT
ATCACACCTC
TCATTGGAGC
TGTTATAGCT
GATTCTTACT
GGGGAAGATA
CTGGACCATT
GCTTCTTTCT
CTGCTATTTA
TTTCATTGGA
ATGGCTTTGT
TGACACTCTC
GGCTTCACTT
CCGGTTTTAA
AGCCAGCGGC
ATGTGCTGGC
GTTGCTGCGG
CTCTATGCTC
ACCAGCAACA
ACGGTTCAGT
ATGCGGTTTT
CTTTACAGGG
CTTTACCTTA
TTGCTCTTGG
TACTGGAGGT
ATCAAACCAT
GTGTATCATC
TTTTGGTGCT
GATCAGTTTG
ATGATACTGA
CCCGAGGGAA
CGAGTTAGAA
AAGCTTCTTT
CTTTAACTGG
TTTTACTTCT
CCATCAACAT
TGGTTCATTT
ATCTCATCCA
CTTTGTTAGT
TTGGGTTCAA
GAAAATGTCG
GGTGGGGACT
CGGTTTCTTG
ATTCCCACCG
TGTTTATGGG
AGTCTCTATT
GCGAGTTTCT
TCATTGGAAC
TCCGCTTTAT
CGGTTTCAGA
AACCGGGAGG
TAGTCCGATT
ACTAGAGTTT
GCCAAGTTCT
TGTAGCTGCA
TACCGTAAAT
TGAAGCTTAA
TCTCCCTGAA
GATATCTCAT
TTCTGTATGA
AACACGAGAG
AAAAACTCCA
TGATTGCTGG
AAGCAGGAAG
ATTCAACACA
CTGACGGTTA
CAAGTTTCTT
GACAAAGCTG
CTGTTATCTC
AGAATATGAA
TCTAAATCCG
GTGCTTTTTC
CAACCCATGG
AAGCTATGTA
CTGTAACTCA
AGTCGAAGAA
GTTAAGACTC
TAATCCGTAT
GTTTCCAATC
TGGGCCTCGG
GGATCGTCTA
CTCGGTCCTA
TATTCACAGA
TTTCCACTCT
ATTCGTGCAA
CAAGGACGGT
CCATGAACCG
AATAATCCGT
TCTTTCGAGA
TCCCTCCTGC
TTCATTCGGG
GTTTTCGACA
CACTTATCGT
GCTCATATCC
ATCCCAATAT
ATGACCGTTT
CCTCGTACCC
TTTGTAAGAC
GGTTCACAGG
GATACCAAAA
GGGTTAACTG
ATCTGCAGCG
AATGGGCATT
GGTCTTTTTC
TCTCTGTCCT
AAGCATTGCA
GCTGCAGCTA
TTGTTGAGAC
AGTGCGGTTG
CAGCTTGCTC
AAGACTTTGT
TGCAATGAGC
ATATTTTGGC
AGATCCCTCA
GTACATACTA
ATGGGAATAG
CAGAGGTCTT
TTTCTTCATT
GGTCGAGTTG
AGTTTTTCTA
TGATGAATCT
CCTGATGCAA
TGAGAAGTGT
ATGCAGCGCT
TTGGCACTAC
TCAATACCGC
AGTCGGAAGC
TACCTGAGCT
CGTTGATCCT
CACTCTTGTG
GCGTATTTCA
CAGCTCTTGG
AGGTAAAGAT
GGTTGGGTTC
CAGACGACCT
TAATAAAGGA
CATCTTGACT
ACTTCTTCTG
GCTTTTGGTC
TCTCTTGGAC
TTGTCAACAT
TCCTGTTTAT
GCCTTGATTT
GTGTTAAGCA
CACAAAGAAG
AAGGCTTTGT
AAGAGCTCGA
ATTTCCCCGA
TCGTTCAAAC
ATTTGGCAAT
AAAGTTTCTT
AAGATTGAAT
CCTGTTGCCG
GTCTTGCGAT
GATTATCATA
TAATTTCTGT
TGAATTACGT
TAAGCATGTA
ATAATTAACA
TGTAATGCAT
GACGTTATTT
ATGAGATGGG
TTTTTATGAT
TAGAGTCCCG
CAATTATACA
TTTAATACGC
GATAGAAAAC
AAAATATAGC
GCGCAAACTA
GGATAAATTA
TCGCGCGCGG
TGTCATCTAT
GTTACTAGAT
CGGGAATTCA
CTGGCCGTCG
TTTTACAACG
TCGTGACTGG
GAAAACCCTG
GCGTTACCCA
ACTTAATCGC
CTTGCAGCAC
ATCCCCCTTT
CGCCAGCTGG
CGTAATAGCG
AAGAGGCCCG
CACCGATCGC
CCTTCCCAAC
AGTTGCGCAG
CCTGAATGGC
GCCCGCTCCT
TTCGCTTTCT
TCCCTTCCTT
TCTCGCCACG
TTCGCCGGCT
TTCCCCGTCA
AGCTCTAAAT
CGGGGGCTCC
CTTTAGGGTT
CCGATTTAGT
GCTTTACGGC
ACCTCGACCC
CAAAAAACTT
GATTTGGGTG
ATGGTTCACG
TAGTGGGCCA
TCGCCCTGAT
AGACGGTTTT
TCGCCCTTTG
ACGTTGGAGT
CCACGTTCTT
TAATAGTGGA
CTCTTGTTCC
AAACTGGAAC
AACACTCAAC
CCTATCTCGG
GCTATTCTTT
TGATTTATAA
GGGATTTTGC
CGATTTCGGA
ACCACCATCA
AACAGGATTT
TCGCCTGCTG
GGGCAAACCA
GCGTGGACCG
CTTGCTGCAA
CTCTCTCAGG
GCCAGGCGGT
GAAGGGCAAT
CAGCTGTTGC
CCGTCTCACT
GGTGAAAAGA
AAAACCACCC
CAGTACATTA
AAAACGTCCG
CAATGTGTTA
TTAAGTTGTC
TAAGCGTCAA
TTTGTTTACA
CCACAATATA
TCCTGCCACC
AGCCAGCCAA
CAGCTCCCCG
ACCGGCAGCT
CGGCACAAAA
TCACCACTCG
ATACAGGCAG
CCCATCAGTC
CGGGACGGCG
TCAGCGGGAG
AGCCGTTGTA
AGGCGGCAGA
CTTTGCTCAT
GTTACCGATG
CTATTCGGAA
GAACGGCAAC
TAAGCTGCCG
GGTTTGAAAC
ACGGATGATC
TCGCGGAGGG
TAGCATGTTG
ATTGTAACGA
TGACAGAGCG
TTGCTGCCTG
TGATCAAATA
TCATCTCCCT
CGCAGAGATC
CGAATTATCA
GCCTTCTTAT
TCATTTCTCG
CTTAACCGTG
ACAGGCTGTC
GATCTTGAGA
ACTATGCCGA
CATAATAGGA
AATCGCTGGA
TAAAGCCGCT
GAGGAAGCTG
AGTGGCGCTA
TTTCTTTAGA
AGTGAACGTT
GACGATATCA
ACTCCCCTAT
CCATTGCTCA
CCGAATGGTA
CAGGTCGGGG
ACCCGAAGTT
CCGACTGTCG
GCCTGATGCA
TCCCCGGCTG
ATCGACCCCA
GATCTGGGGC
TGAGAAAGCC
CAGTAAGGAA
ACAACTGTAG
GTTCGAGTCG
CGAGATCCCC
CGGAACCAAA
GGAAGTAGGT
TAAACCCGCT
CCGATCAGGC
CGAGCCACGC
CAGGCCGAGA
ACATTGGTTC
CTGTAGGCAT
CGGGATTGGC
GGATCAAACA
CTAAAGCTAC
TGGAACGAGC
AGAAGTCCTC
CGGCCGCCAG
TTGCCAGGCG
GTAAAGGTGA
GCAGAGGCAC
GGGAGGTTGC
CACTTGCGGG
TCAGCACGGT
TCCGAACGCC
ATGGAAACCG
CCCCCGCCAG
GCCCGCTGCG
ACGCCGACAG
GATCTAGCGC
TGCGTTTGGT
GTCAACACCA
ACAGCGCCAC
GCCCGCAGTT
CCGCAAATAG
CCCCCAGGAC
CGCCATCAAT
CGTATCGGGC
TACCTAGCAG
AGCGGCAGAG
ATGAACACGA
CCATCAGCGG
CTGCACAGCG
CCTACCGTCG
CCGCGACCCC
GCCCGGCAGG
CGGTAGACCG
AAATAAACAA
CAAGCTCCAG
AATAGCGAAA
TATTAAGTGC
GCCGAGGATG
AAGATGCGCA
TCCACCAGAT
TCCCGTTGGA
ATCTGTCGGA
CGATCATCAC
GAGCAATAAA
CCCGCCGGCA
ACGCCCGCAG
CAGCATACCG
GCGACCCCTC
GGCCTCGCTG
TTCGGGCTCC
ACGAAAACGC
CGGACAGATG
CGCCTTGTGA
GCGTCCTTGG
GGCCGTCCTC
CTGTTTGAAG
ACCGACAGCC
CAATGATCTC
GCCGTCGATG
TAGGCGCCGA
ATGCCACGGC
ATCTCGCAAC
CGTTCAGCGA
ACGCCTCCAT
GGGCTTTTTC
TCCTCGTGCT
CGTAAACGGA
CCCGAACATC
TCTGGAGCTT
TCTTCAGGGC
CGACAATCGG
ATCTCGCGGA
AATCCTGCAC
GTCGGCCGCT
CCAAGCCGTC
GAATCTGAGC
CTTAATCACA
ATTGTCAATT
TTAATCCTCT
GTTTATCGGC
AGTTCGTAGA
GCGCGCCGTG
CGTCCCGAGC
GATACTGAGC
GAAGCAAGTG
CGTCGAGCAG
TGCCCGCTTG
TTCCTGAAAT
GCCAGTAAAG
CGCTGGCTGC
TGAACCCCCA
GCCGGAACTG
ACCCCACAAG
GCCCTAGCGT
TTGCAATGCA
CCAGGTCATC
ATTGACCCAG
GCGTGTTCCA
CCAGGCCGCT
GCCTCGCAAC
TCTTCGCAGG
CTTCGCCGAC
CTGCTCGCGC
CACTTCTTCA
CGCGGGTGGA
ATCCGATCCG
CACATGAGGC
GGAAGGTTTC
CAGCTTGAGC
GGGTACGGCT
CCCGGTGCGA
GCTGAAATAG
TCGAACATCC
GTCGGGCCGT
CGGCGACAGC
TTGCGGTACT
TCTCCCATAT
GAATTTCGTG
TAGTGGTCGC
CAGCAAACAG
CACGACGATT
TCCTCGTCGA
TCAGGACCTG
GCAACGGGAC
GTTTTCTTGC
CACGGTCCAG
GACGCGGAAG
CGGTGCAGCA
GCGACACCGA
TTCCAGGTGC
CCAACGCGGT
CGGACGTGAA
GCCCATCGCC
GTCGCCTGTA
GGCGCGACAG
GCATTCCTCG
GCCTTCGTGT
AATACCGGCC
ATTGATCGAC
CAGCCCAGGT
CCTGGCAAAG
CTCGTAGAAC
GTGAAGGTGA
TCGGCTCGCC
GATAGGGGTG
CGCTTCGCGT
ACTCCAACAC
CTGCTGCCAC
ACCAGTTCGT
CATCGTCGGC
CCGCAGCTCG
ACGCCGGTGT
AGGTGATCTT
CACGTCCTTG
TTGACGTGGA
AAATGACCTT
GTTTTGCAGC
GCCTCGCGCG
GGATTTTCTT
GTTGCGCGTG
GTGAACAGGG
CAGAGCGGGC
CGTGTCGTTT
GGCATCGCTC
GCATCGTGTC
CGGCCACGGC
GCAATATCGA
ACAAGGAAAG
CTGCATTTCC
TTGATCTGCT
GCTTCGTGTG
TTTCAGCAAC
GCGGCCTGCT
TGGCCTCGCT
GACCTGTTTT
GCCAGGTCCT
CGCCGGCGGT
TTTTCGCTTC
TTGGTCGTCA
TAGTTCCTCG
CGTGTCGATG
GTCATCGACT
TCGCCAAACC
TGCCGCCTCC
TGTTCGAGAC
GACGCGAACG
CTCCACGGCG
GCCGATGGCG
CGGGCAGGGC
AGGGGGAGCC
AGTTGCACGC
TGTCGCGCTC
GATCTTGGCC
GTAGCTTGCT
GGACCATCGA
GCCGACGGAC
TGGAAGGTTT
CGCGGGGCGC
ACGCATGACG
GTGCGGCTTG
CGATGGTTTC
GGCATCCTCG
GCGGAAAACC
CCGCGTCGAT
CAGTTCTTGC
CTGTATGCCT
TCCGGTCAAA
CGTCCGATTC
ATTCACCCTC
CTTGCGGGAT
TGCCCCGACT
CACGCCGGGG
CAATGTGCCC
TTATTCCTGA
TTTGACCCGC
CTGGTGCCTT
GGTGTCCAGA
TAATCCACCT
TATCGGCAAT
GAAGTCGGTC
CCGTAGACCG
TCTGGCCGTC
CTTCTCGTAC
TTGGTATTCC
GAATCTTGCC
CTGCACGAAT
ACCAGCGACC
CCTTGCCCAA
ATACTTGCCG
TGGGCCTCGG
CCTGAGAGCC
AAAACACTTG
ATGCGGAAGA
AGTCGGTGCG
CTCCTGCTTG
TCGCCGGCAT
CGTTGCGCCA
CATCTAGGTA
CTAAAACAAT
TCATCCAGTA
AAATATAATA
TTTTATTTTC
TCCCAATCAG
GCTTGATCCC
CAGTAAGTCA
AAAAATAGCT
CGACATACTG
TTCTTCCCCG
ATATCCTCCC
TGATCGACCG
GACGCAGAAG
GCAATGTCAT
ACCACTTGTC
CGCCCTGCCG
CTTCTCCCAA
GATCAATAAA
GCCACTTACT
TTGCCATCTT
TCACAAAGAT
GTTGCTGTCT
CCCAGGTCGC
CGTGGGAAAA
GACAAGTTCC
TCTTCGGGCT
TTTCCGTCTT
TAAAAAATCA
TACAGCTCGC
GCGGATCTTT
AAATGGAGTG
TCTTCTTCCC
AGTTTTCGCA
ATCCACATCG
GCCAGATCGT
TATTCAGTAA
GTAATCCAAT
TCGGCTAAGC
GGCTGTCTAA
GCTATTCGTA
TAGGGACAAT
CCGATATGTC
GATGGAGTGA
AAGAGCCTGA
TGCACTCCGC
ATACAGCTCG
ATAATCTTTT
CAGGGCTTTG
TTCATCTTCA
TACTCTTCCG
AGCAAAGGAC
GCCATCGGCC
TCACTCATGA
GCAGATTGCT
CCAGCCATCA
TGCCGTTCAA
AGTGCAGGAC
CTTTGGAACA
GGCAGCTTTC
CTTCCAGCCA
TAGCATCATG
TCCTTTTCCC
GTTCCACATC
ATAGGTGGTC
CCTTTATACC
GGCTGTCCGT
CATTTTTAAA
TATAGGTTTT
CATTTTCTCC
CACCAGCTTA
TATACCTTAG
CAGGAGACAT
TCCTTCCGTA
TCTTTTACGC
AGCGGTATTT
TTCGATCAGT
TTTTTCAATT
CCGGTGATAT
TCTCATTTTA
GCCATTTATT
ATTTCCTTCC
TCTTTTCTAC
AGTATTTAAA
GATACCCCAA
GAAGCTAATT
ATAACAAGAC
GAACTCCAAT
TCACTGTTCC
TTGCATTCTA
AAACCTTAAA
TACCAGAAAA
CAGCTTTTTC
AAAGTTGTTT
TCAAAGTTGG
CGTATAACAT
AGTATCGACG
GAGCCGATTT
TGAAACCACA
ATTATGGGTG
ATGCTGCCAA
CTTACTGATT
TAGTGTATGA
TGGTGTTTTT
GAGGTGCTCC
AGTGGCTTCT
GTGTCTATCA
GCTGTCCCTC
CTGTTCAGCT
ACTGACGGGG
TGGTGCGTAA
CGGCAAAAGC
ACCGCCGGAC
ATCAGCGCTA
TCTCTGCTCT
CACTGCCGTA
AAACATGGCA
ACTGCAGTTC
ACTTACACCG
CTTCTCAACC
CGGTACGCAC
CAGAAAATCA
TTGATATGGC
CATGAATGGC
GTTGGATGCC
GGGCAACAGC
CCGCATTATG
GGCGTTGGCC
TCAACACGAT
TTTACGTCAC
TTAAAAAACT
CAGGCCGCAG
TCGGTAACCT
CGCGCATACA
GCCGGGCAGT
GACGTCATCG
TCTGCGCGGA
AATGGACGAA
CAGTGGGGCT
ATGTCGGGGC
TAAATCGCGC
CAGCGCTGGC
TGTTTTACGC
GTATGACAGT
CTCCGGAAGA
CGGTTGTTGC
GCACGTATTC
GGTGAACGCA
CTATGGCGAC
GCTGGGGCGT
CTTATGAGCC
TGCTGTCACC
CTTTGACGTG
GTGATATGGA
TGACGGATGG
CTGGCCGCTG
TATGAATCCC
GCCTGAAGGG
AAAGCTGCAC
GTAATCAGCA
AGCGATATAC
GCAGCGAATT
GAGCGGCATA
ACCTGAATCT
GAGGCAGCAC
CTGGCACGGC
TGGGACGGAA
GTCGCTGTCG
TTCTCAAAAT
CGGTGGAGCT
GCATGACAAA
GTCATCGGGC
ATTATCTGAA
CATAAAACAC
TATCAATAAG
TTGGAGTCAT
TACCCAATTA
TGATAGAATT
TACAAGCTAT
AAGGTTATTG
TCCTGGGTTT
CAAGCATTAG
TCCATGCAAG
TTTTTATGCT
TTGCCCATTC
TATAGATATA
TTGATAAGCG
CGCTGCCTAT
GCCTTGCCCC
CTGAAATCCT
TACATACGGC
GATATCTTCT
ATATAAAAGA
TATATTATCT
TATCAGTATT
GTCAATATAT
TCAAGGCAAT
CTGCCTCCTC
ATCCTCTTCA
TCCTCTTCGT
CTTGGTAGCT
TTTTAAATAT
GGCGCTTCAT
AGAGTAATTC
TGTAAAGGTC
CAATTCTCGT
TTTCATACCT
CGGTATAATC
TTACCTATCA
CCTCAAATGG
TTCGCTGGGT
TTATCGCACC
CCCGAACACG
AGCACGGCAC
CCGCGACCAC
TATGCCAAGA
ATGCCCAAGG
TAAAAATTGC
CGGCCCCGCC
ATGAAGTCCG
TGAATGCCCC
GACGGCCGAA
GTGAAGGGCA
GGCCGCCACC
CAGGCCGCCG
CCCTCACTGC
CCGGCACCTG
GTCGCTGAAT
GTCGATGCCA
GCACCTGCGG
CACGTCAATG
CTTCCGGGCG
TCGCGCTCGG
GCTGATCGCC
CATCCCGTTA
CTGCCCCGAT
CCCGGCAATG
GCAAGGACTG
CCAGCGCTGC
CATTTTTGGG
GTGAGGCCGT
TCGCGGCCGA
GGGGCGCAGC
CCCTGGGGGG
ATGGGAGGCC
CGCGTTAGCG
GGCCGGGAGG
GTTCGAGAAG
GGGGGGCACC
CCCCTTCGGC
GTGCGCGGTC
ACGCGCACAG
GGCGCAGCCC
TGGTTAAAAA
CAAGGTTTAT
AAATATTGGT
TTAAAAGCAG
GTTAAAAGAC
AGGTTAGCGG
TGGCCGAAAA
ACGGGCGGAA
ACCCTTGCAA
ATGCTGGATT
TTCTGCCTGT
GGACAGCCCC
TCAAATGTCA
ATAGGTGCGC
CCCTCATCTG
TCAGCACTCT
GCCCCTCAAG
TGTCAAGGAT
CGCGCCCCTC
ATCTGTCAGT
AGTCGCGCCC
CTCAAGTGTC
AATACCGCAG
GGCACTTATC
CCCAGGCTTG
TCCACATCAT
CTGTGGGAAA
CTCGCGTAAA
ATCAGGCGTT
TTCGCCGATT
TGCGAGGCTG
GCCAGCTCCA
CGTCGCCGGC
CGAAATCGAG
CCTGCCCCTC
ATCTGTCAAC
GCCGCGCCGG
GTGAGTCGGC
CCCTCAAGTG
TCAACGTCCG
CCCCTCATCT
GTCAGTGAGG
GCCAAGTTTT
CCGCGAGGTA
TCCACAACGC
CGGCGGCCGC
GGTGTCTCGC
ACACGGCTTC
GACGGCGTTT
CTGGCGCGTT
TGCAGGGCCA
TAGACGGCCG
CCAGCCCAGC
GGCGAGGGCA
ACCAGCCCGG