名称: | pGWB12 |
---|---|
别称: | |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 蛋白表达 |
片段类型: | ORF,GateWay |
片段物种: | 空载体 |
原核抗性: | Kan |
筛选标记: | Hyg,Neo/G418 |
荧光标记: | |
启动子: | 35s |
复制子: | oriV |
拷贝数: | |
感受态: | DB3.1 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGAGCGTCGC
AAAGGCGCTC
GGTCTTGCCT
TGCTCGTCGG
TGATGTACTT
CACCAGCTCC
GCGAAGTCGC
TCTTCTTGAT
GGAGCGCATG
GGGACGTGCT
TGGCAATCAC
GCGCACCCCC
CGGCCGTTTT
AGCGGCTAAA
AAAGTCATGG
CTCTGCCCTC
GGGCGGACCA
CGCCCATCAT
GACCTTGCCA
AGCTCGTCCT
GCTTCTCTTC
GATCTTCGCC
AGCAGGGCGA
GGATCGTGGC
ATCACCGAAC
CGCGCCGTGC
GCGGGTCGTC
GGTGAGCCAG
AGTTTCAGCA
GGCCGCCCAG
GCGGCCCAGG
TCGCCATTGA
TGCGGGCCAG
CTCGCGGACG
TGCTCATAGT
CCACGACGCC
CGTGATTTTG
TAGCCCTGGC
CGACGGCCAG
CAGGTAGGCC
GACAGGCTCA
TGCCGGCCGC
CGCCGCCTTT
TCCTCAATCG
CTCTTCGTTC
GTCTGGAAGG
CAGTACACCT
TGATAGGTGG
GCTGCCCTTC
CTGGTTGGCT
TGGTTTCATC
AGCCATCCGC
TTGCCCTCAT
CTGTTACGCC
GGCGGTAGCC
GGCCAGCCTC
GCAGAGCAGG
ATTCCCGTTG
AGCACCGCCA
GGTGCGAATA
AGGGACAGTG
AAGAAGGAAC
ACCCGCTCGC
GGGTGGGCCT
ACTTCACCTA
TCCTGCCCGG
CTGACGCCGT
TGGATACACC
AAGGAAAGTC
TACACGAACC
CTTTGGCAAA
ATCCTGTATA
TCGTGCGAAA
AAGGATGGAT
ATACCGAAAA
AATCGCTATA
ATGACCCCGA
AGCAGGGTTA
TGCAGCGGAA
AAGCGCCACG
CTTCCCGAAG
GGAGAAAGGC
GGACAGGTAT
CCGGTAAGCG
GCAGGGTCGG
AACAGGAGAG
CGCACGAGGG
AGCTTCCAGG
GGGAAACGCC
TGGTATCTTT
ATAGTCCTGT
CGGGTTTCGC
CACCTCTGAC
TTGAGCGTCG
ATTTTTGTGA
TGCTCGTCAG
GGGGGCGGAG
CCTATGGAAA
AACGCCAGCA
ACGCGGCCTT
TTTACGGTTC
CTGGCCTTTT
GCTGGCCTTT
TGCTCACATG
TTCTTTCCTG
CGTTATCCCC
TGATTCTGTG
GATAACCGTA
TTACCGCCTT
TGAGTGAGCT
GATACCGCTC
GCCGCAGCCG
AACGACCGAG
CGCAGCGAGT
CAGTGAGCGA
GGAAGCGGAA
GAGCGCCAGA
AGGCCGCCAG
AGAGGCCGAG
CGCGGCCGTG
AGGCTTGGAC
GCTAGGGCAG
GGCATGAAAA
AGCCCGTAGC
GGGCTGCTAC
GGGCGTCTGA
CGCGGTGGAA
AGGGGGAGGG
GATGTTGTCT
ACATGGCTCT
GCTGTAGTGA
GTGGGTTGCG
CTCCGGCAGC
GGTCCTGATC
AATCGTCACC
CTTTCTCGGT
CCTTCAACGT
TCCTGACAAC
GAGCCTCCTT
TTCGCCAATC
CATCGACAAT
CACCGCGAGT
CCCTGCTCGA
ACGCTGCGTC
CGGACCGGCT
TCGTCGAAGG
CGTCTATCGC
GGCCCGCAAC
AGCGGCGAGA
GCGGAGCCTG
TTCAACGGTG
CCGCCGCGCT
CGCCGGCATC
GCTGTCGCCG
GCCTGCTCCT
CAAGCACGGC
CCCAACAGTG
AAGTAGCTGA
TTGTCATCAG
CGCATTGACG
GCGTCCCCGG
CCGAAAAACC
CGCCTCGCAG
AGGAAGCGAA
GCTGCGCGTC
GGCCGTTTCC
ATCTGCGGTG
CGCCCGGTCG
CGTGCCGGCA
TGGATGCGCG
CGCCATCGCG
GTAGGCGAGC
AGCGCCTGCC
TGAAGCTGCG
GGCATTCCCG
ATCAGAAATG
AGCGCCAGTC
GTCGTCGGCT
CTCGGCACCG
AATGCGTATG
ATTCTCCGCC
AGCATGGCTT
CGGCCAGTGC
GTCGAGCAGC
GCCCGCTTGT
TCCTGAAGTG
CCAGTAAAGC
GCCGGCTGCT
GAACCCCCAA
CCGTTCCGCC
AGTTTGCGTG
TCGTCAGACC
GTCTACGCCG
ACCTCGTTCA
ACAGGTCCAG
GGCGGCACGG
ATCACTGTAT
TCGGCTGCAA
CTTTGTCATG
CTTGACACTT
TATCACTGAT
AAACATAATA
TGTCCACCAA
CTTATCAGTG
ATAAAGAATC
CGCGCGTTCA
ATCGGACCAG
CGGAGGCTGG
TCCGGAGGCC
AGACGTGAAA
CCCAACATAC
CCCTGATCGT
AATTCTGAGC
ACTGTCGCGC
TCGACGCTGT
CGGCATCGGC
CTGATTATGC
CGGTGCTGCC
GGGCCTCCTG
CGCGATCTGG
TTCACTCGAA
CGACGTCACC
GCCCACTATG
GCATTCTGCT
GGCGCTGTAT
GCGTTGGTGC
AATTTGCCTG
CGCACCTGTG
CTGGGCGCGC
TGTCGGATCG
TTTCGGGCGG
CGGCCAATCT
TGCTCGTCTC
GCTGGCCGGC
GCCAGATCTG
GGGAACCCTG
TGGTTGGCAT
GCACATACAA
ATGGACGAAC
GGATAAACCT
TTTCACGCCC
TTTTAAATAT
CCGATTATTC
TAATAAACGC
TCTTTTCTCT
TAGGTTTACC
CGCCAATATA
TCCTGTCAAA
CACTGATAGT
TTAAACTGAA
GGCGGGAAAC
GACAATCTGA
TCATGAGCGG
AGAATTAAGG
GAGTCACGTT
ATGACCCCCG
CCGATGACGC
GGGACAAGCC
GTTTTACGTT
TGGAACTGAC
AGAACCGCAA
CGTTGAAGGA
GCCACTCAGC
CGCGGGTTTC
TGGAGTTTAA
TGAGCTAAGC
ACATACGTCA
GAAACCATTA
TTGCGCGTTC
AAAAGTCGCC
TAAGGTCACT
ATCAGCTAGC
AAATATTTCT
TGTCAAAAAT
GCTCCACTGA
CGTTCCATAA
ATTCCCCTCG
GTATCCAATT
AGAGTCTCAT
ATTCACTCTC
AATCCAAATA
ATCTGCACCG
GATCTGGATC
GTTTCGCATG
ATTGAACAAG
ATGGATTGCA
CGCAGGTTCT
CCGGCCGCTT
GGGTGGAGAG
GCTATTCGGC
TATGACTGGG
CACAACAGAC
AATCGGCTGC
TCTGATGCCG
CCGTGTTCCG
GCTGTCAGCG
CAGGGGCGCC
CGGTTCTTTT
TGTCAAGACC
GACCTGTCCG
GTGCCCTGAA
TGAACTGCAG
GACGAGGCAG
CGCGGCTATC
GTGGCTGGCC
ACGACGGGCG
TTCCTTGCGC
AGCTGTGCTC
GACGTTGTCA
CTGAAGCGGG
AAGGGACTGG
CTGCTATTGG
GCGAAGTGCC
GGGGCAGGAT
CTCCTGTCAT
CTCACCTTGC
TCCTGCCGAG
AAAGTATCCA
TCATGGCTGA
TGCAATGCGG
CGGCTGCATA
CGCTTGATCC
GGCTACCTGC
CCATTCGACC
ACCAAGCGAA
ACATCGCATC
GAGCGAGCAC
GTACTCGGAT
GGAAGCCGGT
CTTGTCGATC
AGGATGATCT
GGACGAAGAG
CATCAGGGGC
TCGCGCCAGC
CGAACTGTTC
GCCAGGCTCA
AGGCGCGCAT
GCCCGACGGC
GATGATCTCG
TCGTGACCCA
TGGCGATGCC
TGCTTGCCGA
ATATCATGGT
GGAAAATGGC
CGCTTTTCTG
GATTCATCGA
CTGTGGCCGG
CTGGGTGTGG
CGGACCGCTA
TCAGGACATA
GCGTTGGCTA
CCCGTGATAT
TGCTGAAGAG
CTTGGCGGCG
AATGGGCTGA
CCGCTTCCTC
GTGCTTTACG
GTATCGCCGC
TCCCGATTCG
CAGCGCATCG
CCTTCTATCG
CCTTCTTGAC
GAGTTCTTCT
GAGCGGGACT
CTGGGGTTCG
AAATGACCGA
CCAAGCGACG
CCCAACCTGC
CATCACGAGA
TTTCGATTCC
ACCGCCGCCT
TCTATGAAAG
GTTGGGCTTC
GGAATCGTTT
TCCGGGACGC
CGGCTGGATG
ATCCTCCAGC
GCGGGGATCT
CATGCTGGAG
TTCTTCGCCC
ACGGGATCTC
TGCGGAACAG
GCGGTCGAAG
GTGCCGATAT
CATTACGACA
GCAACGGCCG
ACAAGCACAA
CGCCACGATC
CTGAGCGACA
ATATGATCGG
GCCCGGCGTC
CACATCAACG
GCGTCGGCGG
CGACTGCCCA
GGCAAGACCG
AGATGCACCG
CGATATCTTG
CTGCGTTCGG
ATATTTTCGT
GGAGTTCCCG
CCACAGACCC
GGATGATCCC
CGATCGTTCA
AACATTTGGC
AATAAAGTTT
CTTAAGATTG
AATCCTGTTG
CCGGTCTTGC
GATGATTATC
ATATAATTTC
TGTTGAATTA
CGTTAAGCAT
GTAATAATTA
ACATGTAATG
CATGACGTTA
TTTATGAGAT
GGGTTTTTAT
GATTAGAGTC
CCGCAATTAT
ACATTTAATA
CGCGATAGAA
AACAAAATAT
AGCGCGCAAA
CTAGGATAAA
TTATCGCGCG
CGGTGTCATC
TATGTTACTA
GATCGGGCCT
CCTGTCAATG
CTGGCGGCGG
CTCTGGTGGT
GGTTCTGGTG
GCGGCTCTGA
GGGTGGTGGC
TCTGAGGGTG
GCGGTTCTGA
GGGTGGCGGC
TCTGAGGGAG
GCGGTTCCGG
TGGTGGCTCT
GGTTCCGGTG
ATTTTGATTA
TGAAAAGATG
GCAAACGCTA
ATAAGGGGGC
TATGACCGAA
AATGCCGATG
AAAACGCGCT
ACAGTCTGAC
GCTAAAGGCA
AACTTGATTC
TGTCGCTACT
GATTACGGTG
CTGCTATCGA
TGGTTTCATT
GGTGACGTTT
CCGGCCTTGC
TAATGGTAAT
GGTGCTACTG
GTGATTTTGC
TGGCTCTAAT
TCCCAAATGG
CTCAAGTCGG
TGACGGTGAT
AATTCACCTT
TAATGAATAA
TTTCCGTCAA
TATTTACCTT
CCCTCCCTCA
ATCGGTTGAA
TGTCGCCCTT
TTGTCTTTGG
CCCAATACGC
AAACCGCCTC
TCCCCGCGCG
TTGGCCGATT
CATTAATGCA
GCTGGCACGA
CAGGTTTCCC
GACTGGAAAG
CGGGCAGTGA
GCGCAACGCA
ATTAATGTGA
GTTAGCTCAC
TCATTAGGCA
CCCCAGGCTT
TACACTTTAT
GCTTCCGGCT
CGTATGTTGT
GTGGAATTGT
GAGCGGATAA
CAATTTCACA
CAGGAAACAG
CTATGACCAT
GATTACGCCA
AGCTTGCATG
CCTGCAGGTC
CCCAGATTAG
CCTTTTCAAT
TTCAGAAAGA
ATGCTAACCC
ACAGATGGTT
AGAGAGGCTT
ACGCAGCAGG
TCTCATCAAG
ACGATCTACC
CGAGCAATAA
TCTCCAGGAA
ATCAAATACC
TTCCCAAGAA
GGTTAAAGAT
GCAGTCAAAA
GATTCAGGAC
TAACTGCATC
AAGAACACAG
AGAAAGATAT
ATTTCTCAAG
ATCAGAAGTA
CTATTCCAGT
ATGGACGATT
CAAGGCTTGC
TTCACAAACC
AAGGCAAGTA
ATAGAGATTG
GAGTCTCTAA
AAAGGTAGTT
CCCACTGAAT
CAAAGGCCAT
GGAGTCAAAG
ATTCAAATAG
AGGACCTAAC
AGAACTCGCC
GTAAAGACTG
GCGAACAGTT
CATACAGAGT
CTCTTACGAC
TCAATGACAA
GAAGAAAATC
TTCGTCAACA
TGGTGGAGCA
CGACACACTT
GTCTACTCCA
AAAATATCAA
AGATACAGTC
TCAGAAGACC
AAAGGGCAAT
TGAGACTTTT
CAACAAAGGG
TAATATCCGG
AAACCTCCTC
GGATTCCATT
GCCCAGCTAT
CTGTCACTTT
ATTGTGAAGA
TAGTGGAAAA
GGAAGGTGGC
TCCTACAAAT
GCCATCATTG
CGATAAAGGA
AAGGCCATCG
TTGAAGATGC
CTCTGCCGAC
AGTGGTCCCA
AAGATGGACC
CCCACCCACG
AGGAGCATCG
TGGAAAAAGA
AGACGTTCCA
ACCACGTCTT
CAAAGCAAGT
GGATTGATGT
GATATCTCCA
CTGACGTAAG
GGATGACGCA
CAATCCCACT
ATCCTTCGCA
AGACCCTTCC
TCTATATAAG
GAAGTTCATT
TCATTTGGAG
AGAACACGGG
GGACTCTAGA
ATGAGCGACT
ACAAGGATGA
CGATGACAAG
GCTGTTATCA
CAAGTTTGTA
CAAAAAAGCT
GAACGAGAAA
CGTAAAATGA
TATAAATATC
AATATATTAA
ATTAGATTTT
GCATAAAAAA
CAGACTACAT
AATACTGTAA
AACACAACAT
ATCCAGTCAC
TATGGCGGCC
CCTCGAGGGG
CCGCATTAGG
CACCCCAGGC
TTTACACTTT
ATGCTTCCGG
CTCGTATAAT
GTGTGGATTT
TGAGTTAGGA
TCCGGCGAGA
TTTTCAGGAG
CTAAGGAAGC
TAAAATGGAG
AAAAAAATCA
CTGGATATAC
CACCGTTGAT
ATATCCCAAT
GGCATCGTAA
AGAACATTTT
GAGGCATTTC
AGTCAGTTGC
TCAATGTACC
TATAACCAGA
CCGTTCAGCT
GGATATTACG
GCCTTTTTAA
AGACCGTAAA
GAAAAATAAG
CACAAGTTTT
ATCCGGCCTT
TATTCACATT
CTTGCCCGCC
TGATGAATGC
TCATCCGGAA
TTCCGTATGG
CAATGAAAGA
CGGTGAGCTG
GTGATATGGG
ATAGTGTTCA
CCCTTGTTAC
ACCGTTTTCC
ATGAGCAAAC
TGAAACGTTT
TCATCGCTCT
GGAGTGAATA
CCACGACGAT
TTCCGGCAGT
TTCTACACAT
ATATTCGCAA
GATGTGGCGT
GTTACGGTGA
AAACCTGGCC
TATTTCCCTA
AAGGGTTTAT
TGAGAATATG
TTTTTCGTCT
CAGCCAATCC
CTGGGTGAGT
TTCACCAGTT
TTGATTTAAA
CGTGGCCAAT
ATGGACAACT
TCTTCGCCCC
CGTTTTCACC
ATGGGCAAAT
ATTATACGCA
AGGCGACAAG
GTGCTGATGC
CGCTGGCGAT
TCAGGTTCAT
CATGCCGTCT
GTGATGGCTT
CCATGTCGGC
AGAATGCTTA
ATGAATTACA
ACAGTACTGC
GATGAGTGGC
AGGGCGGGGC
GTAAACGCGT
GGATCCGGCT
TACTAAAAGC
CAGATAACAG
TATGCGTATT
TGCGCGCTGA
TTTTTGCGGT
ATAAGAATAT
ATACTGATAT
GTATACCCGA
AGTATGTCAA
AAAGAGGTGT
GCTATGAAGC
AGCGTATTAC
AGTGACAGTT
GACAGCGACA
GCTATCAGTT
GCTCAAGGCA
TATATGATGT
CAATATCTCC
GGTCTGGTAA
GCACAACCAT
GCAGAATGAA
GCCCGTCGTC
TGCGTGCCGA
ACGCTGGAAA
GCGGAAAATC
AGGAAGGGAT
GGCTGAGGTC
GCCCGGTTTA
TTGAAATGAA
CGGCTCTTTT
GCTGACGAGA
ACAGGGACTG
GTGAAATGCA
GTTTAAGGTT
TACACCTATA
AAAGAGAGAG
CCGTTATCGT
CTGTTTGTGG
ATGTACAGAG
TGATATTATT
GACACGCCCG
GGCGACGGAT
GGTGATCCCC
CTGGCCAGTG
CACGTCTGCT
GTCAGATAAA
GTCTCCCGTG
AACTTTACCC
GGTGGTGCAT
ATCGGGGATG
AAAGCTGGCG
CATGATGACC
ACCGATATGG
CCAGTGTGCC
GGTCTCCGTT
ATCGGGGAAG
AAGTGGCTGA
TCTCAGCCAC
CGCGAAAATG
ACATCAAAAA
CGCCATTAAC
CTGATGTTCT
GGGGAATATA
AATGTCAGGC
TCCCTTATAC
ACAGCCAGTC
TGCAGGTCGA
CCATAGTGAC
TGGATATGTT
GTGTTTTACA
GTATTATGTA
GTCTGTTTTT
TATGCAAAAT
CTAATTTAAT
ATATTGATAT
TTATATCATT
TTACGTTTCT
CGTTCAGCTT
TCTTGTACAA
AGTGGTGATA
ACCTAGAGCT
CGAATTTCCC
CGATCGTTCA
AACATTTGGC
AATAAAGTTT
CTTAAGATTG
AATCCTGTTG
CCGGTCTTGC
GATGATTATC
ATATAATTTC
TGTTGAATTA
CGTTAAGCAT
GTAATAATTA
ACATGTAATG
CATGACGTTA
TTTATGAGAT
GGGTTTTTAT
GATTAGAGTC
CCGCAATTAT
ACATTTAATA
CGCGATAGAA
AACAAAATAT
AGCGCGCAAA
CTAGGATAAA
TTATCGCGCG
CGGTGTCATC
TATGTTACTA
GATCGGGAAT
TAGCTTCATC
AACGCAAGAC
ATGCGCACGA
CCGTCTGACA
GGAGAGGAAT
TTCCGACGAG
CACAGAAAGG
ACTTGCTCTT
GGACGTAGGC
CTATTTCTCA
GGCACATGTA
TCAAGTGTTC
GGACGTGGGT
TTTCGATGGT
GTATCAGCCG
CCGCCAACTG
GGAGATGAGG
AGGCTTTCTT
GGGGGGCAGT
CAGCAGTTCA
TTTCACAAGA
CAGAGGAACT
TGTAAGGAGA
TGCACTGATT
TATCTTGGCG
CAAACCAGCA
GGACGAATTA
GTGGGAATAG
CCCGCGAATA
TCTAAGTTAT
GCCTGTCGGC
ATGAGCAGAA
ACTTCCAATT
CGAAACAGTT
TGGAGAGGTT
GTTTTTGGGC
ATACCTTTTG
TTAGTCAGCC
TCTCGATTGC
TCATCGTCAT
TACACAGTAC
CGAAGTTTGA
TCGATCTAGT
AACATAGATG
ACACCGCGCG
CGATAATTTA
TCCTAGTTTG
CGCGCTATAT
TTTGTTTTCT
ATCGCGTATT
AAATGTATAA
TTGCGGGACT
CTAATCATAA
AAACCCATCT
CATAAATAAC
GTCATGCATT
ACATGTTAAT
TATTACATGC
TTAACGTAAT
TCAACAGAAA
TTATATGATA
ATCATCGCAA
GACCGGCAAC
AGGATTCAAT
CTTAAGAAAC
TTTATTGCCA
AATGTTTGAA
CGATCTGCTT
CGACGCACTC
CTTCTTTACT
CCACCATCTC
GTCCTTATTG
AAAACGTGGG
TAGCACCAAA
ACGAATCAAG
TCGCTGGAAC
TGAAGTTACC
AATCACGCTG
GATGATTTGC
CAGTTGGATT
AATCTTGCCT
TTCCCCGCAT
GAATAATATT
GATGAATGCA
TGCGTGAGGG
GTATTTCGAT
TTTGGCAATA
GCTGCAATTG
CCGCGACATC
CTCCAACGAG
CATAATTCTT
CAGAAAAATA
GCGATGTTCC
ATGTTGTCAG
GGCATGCATG
ATGCACGTTA
TGAGGTGACG
GTGCTAGGCA
GTATTCCCTC
AAAGTTTCAT
AGTCAGTATC
ATATTCATCA
TTGCATTCCT
GCAAGAGAGA
ATTGAGACGC
AATCCACACG
CTGCGGCAAC
CTTCCGGCGT
TCGTGGTCTA
TTTGCTCTTG
GACGTTGCAA
ACGTAAGTGT
TGGATCCCGG
TCGGCATCTA
CTCTATTCCT
TTGCCCTCGG
ACGAGTGCTG
GGGCGTCGGT
TTCCACTATC
GGCGAGTACT
TCTACACAGC
CATCGGTCCA
GACGGCCGCG
CTTCTGCGGG
CGATTTGTGT
ACGCCCGACA
GTCCCGGCTC
CGGATCGGAC
GATTGCGTCG
CATCGACCCT
GCGCCCAAGC
TGCATCATCG
AAATTGCCGT
CAACCAAGCT
CTGATAGAGT
TGGTCAAGAC
CAATGCGGAG
CATATACGCC
CGGAGCCGCG
GCGATCCTGC
AAGCTCCGGA
TGCCTCCGCT
CGAAGTAGCG
CGTCTGCTGC
TCCATACAAG
CCAACCACGG
CCTCCAGAAG
AAGATGTTGG
CGACCTCGTA
TTGGGAATCC
CCGAACATCG
CCTCGCTCCA
GTCAATGACC
GCTGTTATGC
GGCCATTGTC
CGTCAGGACA
TTGTTGGAGC
CGAAATCCGC
GTGCACGAGG
TGCCGGACTT
CGGGGCAGTC
CTCGGCCCAA
AGCATCAGCT
CATCGAGAGC
CTGCGCGACG
GACGCACTGA
CGGTGTCGTC
CATCACAGTT
TGCCAGTGAT
ACACATGGGG
ATCAGCAATC
GCGCATATGA
AATCACGCCA
TGTAGTGTAT
TGACCGATTC
CTTGCGGTCC
GAATGGGCCG
AACCCGCTCG
TCTGGCTAAG
ATCGGCCGCA
GCGATCGCAT
CCATGGCCTC
CGCGACCGGC
TGCAGAACAG
CGGGCAGTTC
GGTTTCAGGC
AGGTCTTGCA
ACGTGACACC
CTGTGCACGG
CGGGAGATGC
AATAGGTCAG
GCTCTCGCTG
AATTCCCCAA
TGTCAAGCAC
TTCCGGAATC
GGGAGCGCGG
CCGATGCAAA
GTGCCGATAA
ACATAACGAT
CTTTGTAGAA
ACCATCGGCG
CAGCTATTTA
CCCGCAGGAC
ATATCCACGC
CCTCCTACAT
CGAAGCTGAA
AGCACGAGAT
TCTTCGCCCT
CCGAGAGCTG
CATCAGGTCG
GAGACGCTGT
CGAACTTTTC
GATCAGAAAC
TTCTCGACAG
ACGTCGCGGT
GAGTTCAGGC
TTTTTCATAT
CGGGGTCGTC
CTCTCCAAAT
GAAATGAACT
TCCTTATATA
GAGGAAGGGT
CTTGCGAAGG
ATAGTGGGAT
TGTGCGTCAT
CCCTTACGTC
AGTGGAGATA
TCACATCAAT
CCACTTGCTT
TGAAGACGTG
GTTGGAACGT
CTTCTTTTTC
CACGATGCTC
CTCGTGGGTG
GGGGTCCATC
TTTGGGACCA
CTGTCGGCAG
AGGCATCTTG
AACGATAGCC
TTTCCTTTAT
CGCAATGATG
GCATTTGTAG
GTGCCACCTT
CCTTTTCTAC
TGTCCTTTTG
ATGAAGTGAC
AGATAGCTGG
GCAATGGAAT
CCGAGGAGGT
TTCCCGATAT
TACCCTTTGT
TGAAAAGTCT
CAATAGCCCT
TTGGTCTTCT
GAGACTGTAT
CTTTGATATT
CTTGGAGTAG
ACGAGAGTGT
CGTGCTCCAC
CATGTTGACG
GATCTCTAGG
ACGCGTCCTA
GAAGCTAATT
CACTGGCCGT
CGTTTTACAA
CGTCGTGACT
GGGAAAACCC
TGGCGTTACC
CAACTTAATC
GCCTTGCAGC
ACATCCCCCT
TTCGCCAGCT
GGCGTAATAG
CGAAGAGGCC
CGCACCGATC
GCCCTTCCCA
ACAGTTGCGC
AGCCTGAATG
GCGCCCGCTC
CTTTCGCTTT
CTTCCCTTCC
TTTCTCGCCA
CGTTCGCCGG
CTTTCCCCGT
CAAGCTCTAA
ATCGGGGGCT
CCCTTTAGGG
TTCCGATTTA
GTGCTTTACG
GCACCTCGAC
CCCAAAAAAC
TTGATTTGGG
TGATGGTTCA
CGTAGTGGGC
CATCGCCCTG
ATAGACGGTT
TTTCGCCCTT
TGACGTTGGA
GTCCACGTTC
TTTAATAGTG
GACTCTTGTT
CCAAACTGGA
ACAACACTCA
ACCCTATCTC
GGGCTATTCT
TTTGATTTAT
AAGGGATTTT
GCCGATTTCG
GAACCACCAT
CAAACAGGAT
TTTCGCCTGC
TGGGGCAAAC
CAGCGTGGAC
CGCTTGCTGC
AACTCTCTCA
GGGCCAGGCG
GTGAAGGGCA
ATCAGCTGTT
GCCCGTCTCA
CTGGTGAAAA
GAAAAACCAC
CCCAGTACAT
TAAAAACGTC
CGCAATGTGT
TATTAAGTTG
TCTAAGCGTC
AATTTGTTTA
CACCACAATA
TATCCTGCCA
CCAGCCAGCC
AACAGCTCCC
CGACCGGCAG
CTCGGCACAA
AATCACCACT
CGATACAGGC
AGCCCATCAG
TCCGGGACGG
CGTCAGCGGG
AGAGCCGTTG
TAAGGCGGCA
GACTTTGCTC
ATGTTACCGA
TGCTATTCGG
AAGAACGGCA
ACTAAGCTGC
CGGGTTTGAA
ACACGGATGA
TCTCGCGGAG
GGTAGCATGT
TGATTGTAAC
GATGACAGAG
CGTTGCTGCC
TGTGATCAAA
TATCATCTCC
CTCGCAGAGA
TCCGAATTAT
CAGCCTTCTT
ATTCATTTCT
CGCTTAACCG
TGACAGGCTG
TCGATCTTGA
GAACTATGCC
GACATAATAG
GAAATCGCTG
GATAAAGCCG
CTGAGGAAGC
TGAGTGGCGC
TATTTCTTTA
GAAGTGAACG
TTGACGATAT
CAACTCCCCT
ATCCATTGCT
CACCGAATGG
TACAGGTCGG
GGACCCGAAG
TTCCGACTGT
CGGCCTGATG
CATCCCCGGC
TGATCGACCC
CAGATCTGGG
GCTGAGAAAG
CCCAGTAAGG
AAACAACTGT
AGGTTCGAGT
CGCGAGATCC
CCCGGAACCA
AAGGAAGTAG
GTTAAACCCG
CTCCGATCAG
GCCGAGCCAC
GCCAGGCCGA
GAACATTGGT
TCCTGTAGGC
ATCGGGATTG
GCGGATCAAA
CACTAAAGCT
ACTGGAACGA
GCAGAAGTCC
TCCGGCCGCC
AGTTGCCAGG
CGGTAAAGGT
GAGCAGAGGC
ACGGGAGGTT
GCCACTTGCG
GGTCAGCACG
GTTCCGAACG
CCATGGAAAC
CGCCCCCGCC
AGGCCCGCTG
CGACGCCGAC
AGGATCTAGC
GCTGCGTTTG
GTGTCAACAC
CAACAGCGCC
ACGCCCGCAG
TTCCGCAAAT
AGCCCCCAGG
ACCGCCATCA
ATCGTATCGG
GCTACCTAGC
AGAGCGGCAG
AGATGAACAC
GACCATCAGC
GGCTGCACAG
CGCCTACCGT
CGCCGCGACC
CCGCCCGGCA
GGCGGTAGAC
CGAAATAAAC
AACAAGCTCC
AGAATAGCGA
AATATTAAGT
GCGCCGAGGA
TGAAGATGCG
CATCCACCAG
ATTCCCGTTG
GAATCTGTCG
GACGATCATC
ACGAGCAATA
AACCCGCCGG
CAACGCCCGC
AGCAGCATAC
CGGCGACCCC
TCGGCCTCGC
TGTTCGGGCT
CCACGAAAAC
GCCGGACAGA
TGCGCCTTGT
GAGCGTCCTT
GGGGCCGTCC
TCCTGTTTGA
AGACCGACAG
CCCAATGATC
TCGCCGTCGA
TGTAGGCGCC
GAATGCCACG
GCATCTCGCA
ACCGTTCAGC
GAACGCCTCC
ATGGGCTTTT
TCTCCTCGTG
CTCGTAAACG
GACCCGAACA
TCTCTGGAGC
TTTCTTCAGG
GCCGACAATC
GGATCTCGCG
GAAATCCTGC
ACGTCGGCCG
CTCCAAGCCG
TCGAATCTGA
GCCTTAATCA
CAATTGTCAA
TTTTAATCCT
CTGTTTATCG
GCAGTTCGTA
GAGCGCGCCG
TGCGTCCCGA
GCGATACTGA
GCGAAGCAAG
TGCGTCGAGC
AGTGCCCGCT
TGTTCCTGAA
ATGCCAGTAA
AGCGCTGGCT
GCTGAACCCC
CAGCCGGAAC
TGACCCCACA
AGGCCCTAGC
GTTTGCAATG
CACCAGGTCA
TCATTGACCC
AGGCGTGTTC
CACCAGGCCG
CTGCCTCGCA
ACTCTTCGCA
GGCTTCGCCG
ACCTGCTCGC
GCCACTTCTT
CACGCGGGTG
GAATCCGATC
CGCACATGAG
GCGGAAGGTT
TCCAGCTTGA
GCGGGTACGG
CTCCCGGTGC
GAGCTGAAAT
AGTCGAACAT
CCGTCGGGCC
GTCGGCGACA
GCTTGCGGTA
CTTCTCCCAT
ATGAATTTCG
TGTAGTGGTC
GCCAGCAAAC
AGCACGACGA
TTTCCTCGTC
GATCAGGACC
TGGCAACGGG
ACGTTTTCTT
GCCACGGTCC
AGGACGCGGA
AGCGGTGCAG
CAGCGACACC
GATTCCAGGT
GCCCAACGCG
GTCGGACGTG
AAGCCCATCG
CCGTCGCCTG
TAGGCGCGAC
AGGCATTCCT
CGGCCTTCGT
GTAATACCGG
CCATTGATCG
ACCAGCCCAG
GTCCTGGCAA
AGCTCGTAGA
ACGTGAAGGT
GATCGGCTCG
CCGATAGGGG
TGCGCTTCGC
GTACTCCAAC
ACCTGCTGCC
ACACCAGTTC
GTCATCGTCG
GCCCGCAGCT
CGACGCCGGT
GTAGGTGATC
TTCACGTCCT
TGTTGACGTG
GAAAATGACC
TTGTTTTGCA
GCGCCTCGCG
CGGGATTTTC
TTGTTGCGCG
TGGTGAACAG
GGCAGAGCGG
GCCGTGTCGT
TTGGCATCGC
TCGCATCGTG
TCCGGCCACG
GCGCAATATC
GAACAAGGAA
AGCTGCATTT
CCTTGATCTG
CTGCTTCGTG
TGTTTCAGCA
ACGCGGCCTG
CTTGGCCTCG
CTGACCTGTT
TTGCCAGGTC
CTCGCCGGCG
GTTTTTCGCT
TCTTGGTCGT
CATAGTTCCT
CGCGTGTCGA
TGGTCATCGA
CTTCGCCAAA
CCTGCCGCCT
CCTGTTCGAG
ACGACGCGAA
CGCTCCACGG
CGGCCGATGG
CGCGGGCAGG
GCAGGGGGAG
CCAGTTGCAC
GCTGTCGCGC
TCGATCTTGG
CCGTAGCTTG
CTGGACCATC
GAGCCGACGG
ACTGGAAGGT
TTCGCGGGGC
GCACGCATGA
CGGTGCGGCT
TGCGATGGTT
TCGGCATCCT
CGGCGGAAAA
CCCCGCGTCG
ATCAGTTCTT
GCCTGTATGC
CTTCCGGTCA
AACGTCCGAT
TCATTCACCC
TCCTTGCGGG
ATTGCCCCGA
CTCACGCCGG
GGCAATGTGC
CCTTATTCCT
GATTTGACCC
GCCTGGTGCC
TTGGTGTCCA
GATAATCCAC
CTTATCGGCA
ATGAAGTCGG
TCCCGTAGAC
CGTCTGGCCG
TCCTTCTCGT
ACTTGGTATT
CCGAATCTTG
CCCTGCACGA
ATACCAGCGA
CCCCTTGCCC
AAATACTTGC
CGTGGGCCTC
GGCCTGAGAG
CCAAAACACT
TGATGCGGAA
GAAGTCGGTG
CGCTCCTGCT
TGTCGCCGGC
ATCGTTGCGC
CACATCTAGG
TACTAAAACA
ATTCATCCAG
TAAAATATAA
TATTTTATTT
TCTCCCAATC
AGGCTTGATC
CCCAGTAAGT
CAAAAAATAG
CTCGACATAC
TGTTCTTCCC
CGATATCCTC
CCTGATCGAC
CGGACGCAGA
AGGCAATGTC
ATACCACTTG
TCCGCCCTGC
CGCTTCTCCC
AAGATCAATA
AAGCCACTTA
CTTTGCCATC
TTTCACAAAG
ATGTTGCTGT
CTCCCAGGTC
GCCGTGGGAA
AAGACAAGTT
CCTCTTCGGG
CTTTTCCGTC
TTTAAAAAAT
CATACAGCTC
GCGCGGATCT
TTAAATGGAG
TGTCTTCTTC
CCAGTTTTCG
CAATCCACAT
CGGCCAGATC
GTTATTCAGT
AAGTAATCCA
ATTCGGCTAA
GCGGCTGTCT
AAGCTATTCG
TATAGGGACA
ATCCGATATG
TCGATGGAGT
GAAAGAGCCT
GATGCACTCC
GCATACAGCT
CGATAATCTT
TTCAGGGCTT
TGTTCATCTT
CATACTCTTC
CGAGCAAAGG
ACGCCATCGG
CCTCACTCAT
GAGCAGATTG
CTCCAGCCAT
CATGCCGTTC
AAAGTGCAGG
ACCTTTGGAA
CAGGCAGCTT
TCCTTCCAGC
CATAGCATCA
TGTCCTTTTC
CCGTTCCACA
TCATAGGTGG
TCCCTTTATA
CCGGCTGTCC
GTCATTTTTA
AATATAGGTT
TTCATTTTCT
CCCACCAGCT
TATATACCTT
AGCAGGAGAC
ATTCCTTCCG
TATCTTTTAC
GCAGCGGTAT
TTTTCGATCA
GTTTTTTCAA
TTCCGGTGAT
ATTCTCATTT
TAGCCATTTA
TTATTTCCTT
CCTCTTTTCT
ACAGTATTTA
AAGATACCCC
AAGAAGCTAA
TTATAACAAG
ACGAACTCCA
ATTCACTGTT
CCTTGCATTC
TAAAACCTTA
AATACCAGAA
AACAGCTTTT
TCAAAGTTGT
TTTCAAAGTT
GGCGTATAAC
ATAGTATCGA
CGGAGCCGAT
TTTGAAACCA
CAATTATGGG
TGATGCTGCC
AACTTACTGA
TTTAGTGTAT
GATGGTGTTT
TTGAGGTGCT
CCAGTGGCTT
CTGTGTCTAT
CAGCTGTCCC
TCCTGTTCAG
CTACTGACGG
GGTGGTGCGT
AACGGCAAAA
GCACCGCCGG
ACATCAGCGC
TATCTCTGCT
CTCACTGCCG
TAAAACATGG
CAACTGCAGT
TCACTTACAC
CGCTTCTCAA
CCCGGTACGC
ACCAGAAAAT
CATTGATATG
GCCATGAATG
GCGTTGGATG
CCGGGCAACA
GCCCGCATTA
TGGGCGTTGG
CCTCAACACG
ATTTTACGTC
ACTTAAAAAA
CTCAGGCCGC
AGTCGGTAAC
CTCGCGCATA
CAGCCGGGCA
GTGACGTCAT
CGTCTGCGCG
GAAATGGACG
AACAGTGGGG
CTATGTCGGG
GCTAAATCGC
GCCAGCGCTG
GCTGTTTTAC
GCGTATGACA
GTCTCCGGAA
GACGGTTGTT
GCGCACGTAT
TCGGTGAACG
CACTATGGCG
ACGCTGGGGC
GTCTTATGAG
CCTGCTGTCA
CCCTTTGACG
TGGTGATATG
GATGACGGAT
GGCTGGCCGC
TGTATGAATC
CCGCCTGAAG
GGAAAGCTGC
ACGTAATCAG
CAAGCGATAT
ACGCAGCGAA
TTGAGCGGCA
TAACCTGAAT
CTGAGGCAGC
ACCTGGCACG
GCTGGGACGG
AAGTCGCTGT
CGTTCTCAAA
ATCGGTGGAG
CTGCATGACA
AAGTCATCGG
GCATTATCTG
AACATAAAAC
ACTATCAATA
AGTTGGAGTC
ATTACCCAAT
TATGATAGAA
TTTACAAGCT
ATAAGGTTAT
TGTCCTGGGT
TTCAAGCATT
AGTCCATGCA
AGTTTTTATG
CTTTGCCCAT
TCTATAGATA
TATTGATAAG
CGCGCTGCCT
ATGCCTTGCC
CCCTGAAATC
CTTACATACG
GCGATATCTT
CTATATAAAA
GATATATTAT
CTTATCAGTA
TTGTCAATAT
ATTCAAGGCA
ATCTGCCTCC
TCATCCTCTT
CATCCTCTTC
GTCTTGGTAG
CTTTTTAAAT
ATGGCGCTTC
ATAGAGTAAT
TCTGTAAAGG
TCCAATTCTC
GTTTTCATAC
CTCGGTATAA
TCTTACCTAT
CACCTCAAAT
GGTTCGCTGG
GTTTATCGCA
CCCCCGAACA
CGAGCACGGC
ACCCGCGACC
ACTATGCCAA
GAATGCCCAA
GGTAAAAATT
GCCGGCCCCG
CCATGAAGTC
CGTGAATGCC
CCGACGGCCG
AAGTGAAGGG
CAGGCCGCCA
CCCAGGCCGC
CGCCCTCACT
GCCCGGCACC
TGGTCGCTGA
ATGTCGATGC
CAGCACCTGC
GGCACGTCAA
TGCTTCCGGG
CGTCGCGCTC
GGGCTGATCG
CCCATCCCGT
TACTGCCCCG
ATCCCGGCAA
TGGCAAGGAC
TGCCAGCGCT
GCCATTTTTG
GGGTGAGGCC
GTTCGCGGCC
GAGGGGCGCA
GCCCCTGGGG
GGATGGGAGG
CCCGCGTTAG
CGGGCCGGGA
GGGTTCGAGA
AGGGGGGGCA
CCCCCCTTCG
GCGTGCGCGG
TCACGCGCAC
AGGGCGCAGC
CCTGGTTAAA
AACAAGGTTT
ATAAATATTG
GTTTAAAAGC
AGGTTAAAAG
ACAGGTTAGC
GGTGGCCGAA
AAACGGGCGG
AAACCCTTGC
AAATGCTGGA
TTTTCTGCCT
GTGGACAGCC
CCTCAAATGT
CAATAGGTGC
GCCCCTCATC
TGTCAGCACT
CTGCCCCTCA
AGTGTCAAGG
ATCGCGCCCC
TCATCTGTCA
GTAGTCGCGC
CCCTCAAGTG
TCAATACCGC
AGGGCACTTA
TCCCCAGGCT
TGTCCACATC
ATCTGTGGGA
AACTCGCGTA
AAATCAGGCG
TTTTCGCCGA
TTTGCGAGGC
TGGCCAGCTC
CACGTCGCCG
GCCGAAATCG
AGCCTGCCCC
TCATCTGTCA
ACGCCGCGCC
GGGTGAGTCG
GCCCCTCAAG
TGTCAACGTC
CGCCCCTCAT
CTGTCAGTGA
GGGCCAAGTT
TTCCGCGAGG
TATCCACAAC
GCCGGCGGCC
GCGGTGTCTC
GCACACGGCT
TCGACGGCGT
TTCTGGCGCG
TTTGCAGGGC
CATAGACGGC
CGCCAGCCCA
GCGGCGAGGG
CAACCAGCCC
GG