产品货号：P15213
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pCDNA3.1-dCas9 |
|---|---|
| 别称: | |
| 基因长: | |
| 质粒宿主： | 哺乳细胞 |
| 质粒用途： | 基因编辑 |
| 片段类型： | CRISPR |
| 片段物种： | |
| 原核抗性： | Amp |
| 筛选标记： | Neo/G418 |
| 荧光标记： | |
| 启动子: | CMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | DH5a |
| 温度: | 37度 |
| 背景载体: | |
| 正向引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
| 反向引物: | BGH：TAGAAGGCACAGTCGAGG |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
GCACTCTCAG
TACAATCTGC
TCTGATGCCG
CATAGTTAAG
CCAGTATCTG
CTCCCTGCTT
GTGTGTTGGA
GGTCGCTGAG
TAGTGCGCGA
GCAAAATTTA
AGCTACAACA
AGGCAAGGCT
TGACCGACAA
TTGCATGAAG
AATCTGCTTA
GGGTTAGGCG
TTTTGCGCTG
CTTCGCGATG
TACGGGCCAG
ATATACGCGT
TGACATTGAT
TATTGACTAG
TTATTAATAG
TAATCAATTA
CGGGGTCATT
AGTTCATAGC
CCATATATGG
AGTTCCGCGT
TACATAACTT
ACGGTAAATG
GCCCGCCTGG
CTGACCGCCC
AACGACCCCC
GCCCATTGAC
GTCAATAATG
ACGTATGTTC
CCATAGTAAC
GCCAATAGGG
ACTTTCCATT
GACGTCAATG
GGTGGAGTAT
TTACGGTAAA
CTGCCCACTT
GGCAGTACAT
CAAGTGTATC
ATATGCCAAG
TACGCCCCCT
ATTGACGTCA
ATGACGGTAA
ATGGCCCGCC
TGGCATTATG
CCCAGTACAT
GACCTTATGG
GACTTTCCTA
CTTGGCAGTA
CATCTACGTA
TTAGTCATCG
CTATTACCAT
GGTGATGCGG
TTTTGGCAGT
ACATCAATGG
GCGTGGATAG
CGGTTTGACT
CACGGGGATT
TCCAAGTCTC
CACCCCATTG
ACGTCAATGG
GAGTTTGTTT
TGGCACCAAA
ATCAACGGGA
CTTTCCAAAA
TGTCGTAACA
ACTCCGCCCC
ATTGACGCAA
ATGGGCGGTA
GGCGTGTACG
GTGGGAGGTC
TATATAAGCA
GAGCTCTCTG
GCTAACTAGA
GAACCCACTG
CTTACTGGCT
TATCGAAATT
AATACGACTC
ACTATAGGGA
GACCCAAGCT
GGCTAGCGTT
TAAACTTAAG
CTTGGTACCG
CCACCATGAA
AAGGCCGGCG
GCCACGAAAA
AGGCCGGCCA
GGCAAAAAAG
AAAAAGGACA
AGAAGTACAG
CATCGGCCTG
GCCATCGGCA
CCAACTCTGT
GGGCTGGGCC
GTGATCACCG
ACGAGTACAA
GGTGCCCAGC
AAGAAATTCA
AGGTGCTGGG
CAACACCGAC
CGGCACAGCA
TCAAGAAGAA
CCTGATCGGA
GCCCTGCTGT
TCGACAGCGG
CGAAACAGCC
GAGGCCACCC
GGCTGAAGAG
AACCGCCAGA
AGAAGATACA
CCAGACGGAA
GAACCGGATC
TGCTATCTGC
AAGAGATCTT
CAGCAACGAG
ATGGCCAAGG
TGGACGACAG
CTTCTTCCAC
AGACTGGAAG
AGTCCTTCCT
GGTGGAAGAG
GATAAGAAGC
ACGAGCGGCA
CCCCATCTTC
GGCAACATCG
TGGACGAGGT
GGCCTACCAC
GAGAAGTACC
CCACCATCTA
CCACCTGAGA
AAGAAACTGG
TGGACAGCAC
CGACAAGGCC
GACCTGCGGC
TGATCTATCT
GGCCCTGGCC
CACATGATCA
AGTTCCGGGG
CCACTTCCTG
ATCGAGGGCG
ACCTGAACCC
CGACAACAGC
GACGTGGACA
AGCTGTTCAT
CCAGCTGGTG
CAGACCTACA
ACCAGCTGTT
CGAGGAAAAC
CCCATCAACG
CCAGCGGCGT
GGACGCCAAG
GCCATCCTGT
CTGCCAGACT
GAGCAAGAGC
AGACGGCTGG
AAAATCTGAT
CGCCCAGCTG
CCCGGCGAGA
AGAAGAATGG
CCTGTTCGGC
AACCTGATTG
CCCTGAGCCT
GGGCCTGACC
CCCAACTTCA
AGAGCAACTT
CGACCTGGCC
GAGGATGCCA
AACTGCAGCT
GAGCAAGGAC
ACCTACGACG
ACGACCTGGA
CAACCTGCTG
GCCCAGATCG
GCGACCAGTA
CGCCGACCTG
TTTCTGGCCG
CCAAGAACCT
GTCCGACGCC
ATCCTGCTGA
GCGACATCCT
GAGAGTGAAC
ACCGAGATCA
CCAAGGCCCC
CCTGAGCGCC
TCTATGATCA
AGAGATACGA
CGAGCACCAC
CAGGACCTGA
CCCTGCTGAA
AGCTCTCGTG
CGGCAGCAGC
TGCCTGAGAA
GTACAAAGAG
ATTTTCTTCG
ACCAGAGCAA
GAACGGCTAC
GCCGGCTACA
TTGACGGCGG
AGCCAGCCAG
GAAGAGTTCT
ACAAGTTCAT
CAAGCCCATC
CTGGAAAAGA
TGGACGGCAC
CGAGGAACTG
CTCGTGAAGC
TGAACAGAGA
GGACCTGCTG
CGGAAGCAGC
GGACCTTCGA
CAACGGCAGC
ATCCCCCACC
AGATCCACCT
GGGAGAGCTG
CACGCCATTC
TGCGGCGGCA
GGAAGATTTT
TACCCATTCC
TGAAGGACAA
CCGGGAAAAG
ATCGAGAAGA
TCCTGACCTT
CCGCATCCCC
TACTACGTGG
GCCCTCTGGC
CAGGGGAAAC
AGCAGATTCG
CCTGGATGAC
CAGAAAGAGC
GAGGAAACCA
TCACCCCCTG
GAACTTCGAG
GAAGTGGTGG
ACAAGGGCGC
TTCCGCCCAG
AGCTTCATCG
AGCGGATGAC
CAACTTCGAT
AAGAACCTGC
CCAACGAGAA
GGTGCTGCCC
AAGCACAGCC
TGCTGTACGA
GTACTTCACC
GTGTATAACG
AGCTGACCAA
AGTGAAATAC
GTGACCGAGG
GAATGAGAAA
GCCCGCCTTC
CTGAGCGGCG
AGCAGAAAAA
GGCCATCGTG
GACCTGCTGT
TCAAGACCAA
CCGGAAAGTG
ACCGTGAAGC
AGCTGAAAGA
GGACTACTTC
AAGAAAATCG
AGTGCTTCGA
CTCCGTGGAA
ATCTCCGGCG
TGGAAGATCG
GTTCAACGCC
TCCCTGGGCA
CATACCACGA
TCTGCTGAAA
ATTATCAAGG
ACAAGGACTT
CCTGGACAAT
GAGGAAAACG
AGGACATTCT
GGAAGATATC
GTGCTGACCC
TGACACTGTT
TGAGGACAGA
GAGATGATCG
AGGAACGGCT
GAAAACCTAT
GCCCACCTGT
TCGACGACAA
AGTGATGAAG
CAGCTGAAGC
GGCGGAGATA
CACCGGCTGG
GGCAGGCTGA
GCCGGAAGCT
GATCAACGGC
ATCCGGGACA
AGCAGTCCGG
CAAGACAATC
CTGGATTTCC
TGAAGTCCGA
CGGCTTCGCC
AACAGAAACT
TCATGCAGCT
GATCCACGAC
GACAGCCTGA
CCTTTAAAGA
GGACATCCAG
AAAGCCCAGG
TGTCCGGCCA
GGGCGATAGC
CTGCACGAGC
ACATTGCCAA
TCTGGCCGGC
AGCCCCGCCA
TTAAGAAGGG
CATCCTGCAG
ACAGTGAAGG
TGGTGGACGA
GCTCGTGAAA
GTGATGGGCC
GGCACAAGCC
CGAGAACATC
GTGATCGAAA
TGGCCAGAGA
GAACCAGACC
ACCCAGAAGG
GACAGAAGAA
CAGCCGCGAG
AGAATGAAGC
GGATCGAAGA
GGGCATCAAA
GAGCTGGGCA
GCCAGATCCT
GAAAGAACAC
CCCGTGGAAA
ACACCCAGCT
GCAGAACGAG
AAGCTGTACC
TGTACTACCT
GCAGAATGGG
CGGGATATGT
ACGTGGACCA
GGAACTGGAC
ATCAACCGGC
TGTCCGACTA
CGATGTGGAC
CACATCGTGC
CTCAGAGCTT
TCTGAAGGAC
GACTCCATCG
ACAACAAGGT
GCTGACCAGA
AGCGACAAGG
CCCGGGGCAA
GAGCGACAAC
GTGCCCTCCG
AAGAGGTCGT
GAAGAAGATG
AAGAACTACT
GGCGGCAGCT
GCTGAACGCC
AAGCTGATTA
CCCAGAGAAA
GTTCGACAAT
CTGACCAAGG
CCGAGAGAGG
CGGCCTGAGC
GAACTGGATA
AGGCCGGCTT
CATCAAGAGA
CAGCTGGTGG
AAACCCGGCA
GATCACAAAG
CACGTGGCAC
AGATCCTGGA
CTCCCGGATG
AACACTAAGT
ACGACGAGAA
TGACAAGCTG
ATCCGGGAAG
TGAAAGTGAT
CACCCTGAAG
TCCAAGCTGG
TGTCCGATTT
CCGGAAGGAT
TTCCAGTTTT
ACAAAGTGCG
CGAGATCAAC
AACTACCACC
ACGCCCACGA
CGCCTACCTG
AACGCCGTCG
TGGGAACCGC
CCTGATCAAA
AAGTACCCTA
AGCTGGAAAG
CGAGTTCGTG
TACGGCGACT
ACAAGGTGTA
CGACGTGCGG
AAGATGATCG
CCAAGAGCGA
GCAGGAAATC
GGCAAGGCTA
CCGCCAAGTA
CTTCTTCTAC
AGCAACATCA
TGAACTTTTT
CAAGACCGAG
ATTACCCTGG
CCAACGGCGA
GATCCGGAAG
CGGCCTCTGA
TCGAGACAAA
CGGCGAAACC
GGGGAGATCG
TGTGGGATAA
GGGCCGGGAT
TTTGCCACCG
TGCGGAAAGT
GCTGAGCATG
CCCCAAGTGA
ATATCGTGAA
AAAGACCGAG
GTGCAGACAG
GCGGCTTCAG
CAAAGAGTCT
ATCCTGCCCA
AGAGGAACAG
CGATAAGCTG
ATCGCCAGAA
AGAAGGACTG
GGACCCTAAG
AAGTACGGCG
GCTTCGACAG
CCCCACCGTG
GCCTATTCTG
TGCTGGTGGT
GGCCAAAGTG
GAAAAGGGCA
AGTCCAAGAA
ACTGAAGAGT
GTGAAAGAGC
TGCTGGGGAT
CACCATCATG
GAAAGAAGCA
GCTTCGAGAA
GAATCCCATC
GACTTTCTGG
AAGCCAAGGG
CTACAAAGAA
GTGAAAAAGG
ACCTGATCAT
CAAGCTGCCT
AAGTACTCCC
TGTTCGAGCT
GGAAAACGGC
CGGAAGAGAA
TGCTGGCCTC
TGCCGGCGAA
CTGCAGAAGG
GAAACGAACT
GGCCCTGCCC
TCCAAATATG
TGAACTTCCT
GTACCTGGCC
AGCCACTATG
AGAAGCTGAA
GGGCTCCCCC
GAGGATAATG
AGCAGAAACA
GCTGTTTGTG
GAACAGCACA
AGCACTACCT
GGACGAGATC
ATCGAGCAGA
TCAGCGAGTT
CTCCAAGAGA
GTGATCCTGG
CCGACGCTAA
TCTGGACAAA
GTGCTGTCCG
CCTACAACAA
GCACCGGGAT
AAGCCCATCA
GAGAGCAGGC
CGAGAATATC
ATCCACCTGT
TTACCCTGAC
CAATCTGGGA
GCCCCTGCCG
CCTTCAAGTA
CTTTGACACC
ACCATCGACC
GGAAGAGGTA
CACCAGCACC
AAAGAGGTGC
TGGACGCCAC
CCTGATCCAC
CAGAGCATCA
CCGGCCTGTA
CGAGACACGG
ATCGACCTGT
CTCAGCTGGG
AGGCGACAGC
GCTGGAGGAG
GTGGAAGCGG
AGGAGGAGGA
AGCGGAGGAG
GAGGTAGCGG
ACCTAAGAAA
AAGAGGAAGG
TGGCGGCCGC
TGGATCCTAG
AATTCTGCAG
ATATCCAGCA
CAGTGGCGGC
CGCTCGAGTC
TAGAGGGCCC
GTTTAAACCC
GCTGATCAGC
CTCGACTGTG
CCTTCTAGTT
GCCAGCCATC
TGTTGTTTGC
CCCTCCCCCG
TGCCTTCCTT
GACCCTGGAA
GGTGCCACTC
CCACTGTCCT
TTCCTAATAA
AATGAGGAAA
TTGCATCGCA
TTGTCTGAGT
AGGTGTCATT
CTATTCTGGG
GGGTGGGGTG
GGGCAGGACA
GCAAGGGGGA
GGATTGGGAA
GACAATAGCA
GGCATGCTGG
GGATGCGGTG
GGCTCTATGG
CTTCTGAGGC
GGAAAGAACC
AGCTGGGGCT
CTAGGGGGTA
TCCCCACGCG
CCCTGTAGCG
GCGCATTAAG
CGCGGCGGGT
GTGGTGGTTA
CGCGCAGCGT
GACCGCTACA
CTTGCCAGCG
CCCTAGCGCC
CGCTCCTTTC
GCTTTCTTCC
CTTCCTTTCT
CGCCACGTTC
GCCGGCTTTC
CCCGTCAAGC
TCTAAATCGG
GGGCTCCCTT
TAGGGTTCCG
ATTTAGTGCT
TTACGGCACC
TCGACCCCAA
AAAACTTGAT
TAGGGTGATG
GTTCACGTAG
TGGGCCATCG
CCCTGATAGA
CGGTTTTTCG
CCCTTTGACG
TTGGAGTCCA
CGTTCTTTAA
TAGTGGACTC
TTGTTCCAAA
CTGGAACAAC
ACTCAACCCT
ATCTCGGTCT
ATTCTTTTGA
TTTATAAGGG
ATTTTGCCGA
TTTCGGCCTA
TTGGTTAAAA
AATGAGCTGA
TTTAACAAAA
ATTTAACGCG
AATTAATTCT
GTGGAATGTG
TGTCAGTTAG
GGTGTGGAAA
GTCCCCAGGC
TCCCCAGCAG
GCAGAAGTAT
GCAAAGCATG
CATCTCAATT
AGTCAGCAAC
CAGGTGTGGA
AAGTCCCCAG
GCTCCCCAGC
AGGCAGAAGT
ATGCAAAGCA
TGCATCTCAA
TTAGTCAGCA
ACCATAGTCC
CGCCCCTAAC
TCCGCCCATC
CCGCCCCTAA
CTCCGCCCAG
TTCCGCCCAT
TCTCCGCCCC
ATGGCTGACT
AATTTTTTTT
ATTTATGCAG
AGGCCGAGGC
CGCCTCTGCC
TCTGAGCTAT
TCCAGAAGTA
GTGAGGAGGC
TTTTTTGGAG
GCCTAGGCTT
TTGCAAAAAG
CTCCCGGGAG
CTTGTATATC
CATTTTCGGA
TCTGATCAAG
AGACAGGATG
AGGATCGTTT
CGCATGATTG
AACAAGATGG
ATTGCACGCA
GGTTCTCCGG
CCGCTTGGGT
GGAGAGGCTA
TTCGGCTATG
ACTGGGCACA
ACAGACAATC
GGCTGCTCTG
ATGCCGCCGT
GTTCCGGCTG
TCAGCGCAGG
GGCGCCCGGT
TCTTTTTGTC
AAGACCGACC
TGTCCGGTGC
CCTGAATGAA
CTGCAGGACG
AGGCAGCGCG
GCTATCGTGG
CTGGCCACGA
CGGGCGTTCC
TTGCGCAGCT
GTGCTCGACG
TTGTCACTGA
AGCGGGAAGG
GACTGGCTGC
TATTGGGCGA
AGTGCCGGGG
CAGGATCTCC
TGTCATCTCA
CCTTGCTCCT
GCCGAGAAAG
TATCCATCAT
GGCTGATGCA
ATGCGGCGGC
TGCATACGCT
TGATCCGGCT
ACCTGCCCAT
TCGACCACCA
AGCGAAACAT
CGCATCGAGC
GAGCACGTAC
TCGGATGGAA
GCCGGTCTTG
TCGATCAGGA
TGATCTGGAC
GAAGAGCATC
AGGGGCTCGC
GCCAGCCGAA
CTGTTCGCCA
GGCTCAAGGC
GCGCATGCCC
GACGGCGAGG
ATCTCGTCGT
GACCCATGGC
GATGCCTGCT
TGCCGAATAT
CATGGTGGAA
AATGGCCGCT
TTTCTGGATT
CATCGACTGT
GGCCGGCTGG
GTGTGGCGGA
CCGCTATCAG
GACATAGCGT
TGGCTACCCG
TGATATTGCT
GAAGAGCTTG
GCGGCGAATG
GGCTGACCGC
TTCCTCGTGC
TTTACGGTAT
CGCCGCTCCC
GATTCGCAGC
GCATCGCCTT
CTATCGCCTT
CTTGACGAGT
TCTTCTGAGC
GGGACTCTGG
GGTTCGAAAT
GACCGACCAA
GCGACGCCCA
ACCTGCCATC
ACGAGATTTC
GATTCCACCG
CCGCCTTCTA
TGAAAGGTTG
GGCTTCGGAA
TCGTTTTCCG
GGACGCCGGC
TGGATGATCC
TCCAGCGCGG
GGATCTCATG
CTGGAGTTCT
TCGCCCACCC
CAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGTATACCGT
CGACCTCTAG
CTAGAGCTTG
GCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TTTTTTTGTT
TGCAAGCAGC
AGATTACGCG
CAGAAAAAAA
GGATCTCAAG
AAGATCCTTT
GATCTTTTCT
ACGGGGTCTG
ACGCTCAGTG
GAACGAAAAC
TCACGTTAAG
GGATTTTGGT
CATGAGATTA
TCAAAAAGGA
TCTTCACCTA
GATCCTTTTA
AATTAAAAAT
GAAGTTTTAA
ATCAATCTAA
AGTATATATG
AGTAAACTTG
GTCTGACAGT
TACCAATGCT
TAATCAGTGA
GGCACCTATC
TCAGCGATCT
GTCTATTTCG
TTCATCCATA
GTTGCCTGAC
TCCCCGTCGT
GTAGATAACT
ACGATACGGG
AGGGCTTACC
ATCTGGCCCC
AGTGCTGCAA
TGATACCGCG
AGACCCACGC
TCACCGGCTC
CAGATTTATC
AGCAATAAAC
CAGCCAGCCG
GAAGGGCCGA
GCGCAGAAGT
GGTCCTGCAA
CTTTATCCGC
CTCCATCCAG
TCTATTAATT
GTTGCCGGGA
AGCTAGAGTA
AGTAGTTCGC
CAGTTAATAG
TTTGCGCAAC
GTTGTTGCCA
TTGCTACAGG
CATCGTGGTG
TCACGCTCGT
CGTTTGGTAT
GGCTTCATTC
AGCTCCGGTT
CCCAACGATC
AAGGCGAGTT
ACATGATCCC
CCATGTTGTG
CAAAAAAGCG
GTTAGCTCCT
TCGGTCCTCC
GATCGTTGTC
AGAAGTAAGT
TGGCCGCAGT
GTTATCACTC
ATGGTTATGG
CAGCACTGCA
TAATTCTCTT
ACTGTCATGC
CATCCGTAAG
ATGCTTTTCT
GTGACTGGTG
AGTACTCAAC
CAAGTCATTC
TGAGAATAGT
GTATGCGGCG
ACCGAGTTGC
TCTTGCCCGG
CGTCAATACG
GGATAATACC
GCGCCACATA
GCAGAACTTT
AAAAGTGCTC
ATCATTGGAA
AACGTTCTTC
GGGGCGAAAA
CTCTCAAGGA
TCTTACCGCT
GTTGAGATCC
AGTTCGATGT
AACCCACTCG
TGCACCCAAC
TGATCTTCAG
CATCTTTTAC
TTTCACCAGC
GTTTCTGGGT
GAGCAAAAAC
AGGAAGGCAA
AATGCCGCAA
AAAAGGGAAT
AAGGGCGACA
CGGAAATGTT
GAATACTCAT
ACTCTTCCTT
TTTCAATATT
ATTGAAGCAT
TTATCAGGGT
TATTGTCTCA
TGAGCGGATA
CATATTTGAA
TGTATTTAGA
AAAATAAACA
AATAGGGGTT
CCGCGCACAT
TTCCCCGAAA
AGTGCCACCT
GACGTCGACG
GATCGGGAGA
TCTCCCGATC
CCCTATGGT
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