名称: | pKCcas9do |
---|---|
别称: | Plasmid #62552 |
基因长: | |
质粒宿主: | 链霉菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 空载体 |
原核抗性: | Apr |
筛选标记: | |
荧光标记: | |
启动子: | J23119 |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 30度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGTGAGGAGG
CTGGTCCTTT
ACCCTGATGC
CCGAGGGCAT
CAGGGTAAAG
GACCAGCCTC
GCCAACTAGG
AGCGCTACCA
AGGCGAACAC
AACCCAGGAC
TTGGTAGAAC
CCTTCGCCGG
AGAAGTTCAG
ACTCATCGGC
ATGAGCACCC
CTCGCCGCGC
ACTCGGCACC
GGTCCGTCGA
CCACCACCAG
GACGTCGTTG
TCGACGTCGG
CCCCGCGGCT
CCTGCCCGCC
GAACGCGTCG
TCGTCGACGG
CCTGGTGCTC
ATCGACGAGC
ACCCGGAGCC
AGGTGAAAAG
CGCCGGCGGA
CGCTCGGACT
GGGCGCGGGA
TTCCAGCAGT
AACCCAGGTC
CGCCGGCCAC
CTCACGGCAG
GCAGACCCTC
GGCTTTCGCG
CCGGGACCGC
CATGAGACCG
CCACCCGGAT
GTCCGGGGTG
GCGGTCTCAT
GGCGGTCCCT
CAGCGGCCCT
ACGCGACCGC
TGTGTCGACG
CGGAGGCAGT
CTCCGGGGTG
CTGGCCCCAG
GGCYGGAKKW
CCGGGGYGGC
GGTCTCATGG
CGGTCCCTCA
GCGGCCCTAC
GCGACCGCTG
TGTCGACGCG
GAGGCAGTCT
CCGGGGTGCT
GGCCCCAGGG
CTGGAGTACC
GGGGCGAGCT
TGCCCTTGCA
GCGGCGGCAC
ACGGGCGACG
CGGCGCCGGG
CGGGGGAGTC
TTGACGTCGA
CGGTGACCGG
CTTCGGCTTC
AGGCGCTTCC
GAAGGCTGAT
CGTCGGGCGG
ATCGCCTCCT
TCTTCGGCTG
ACGCACTCGG
TCGAGCGAAG
CGGCCAGCTC
CTCCGTGCGG
GCCTCGTTGG
CCTTCCGGCG
CGCCTCGCGG
AAAGCAGCTT
CCTCCTCGGA
CATGACCTCG
AACCTCATCT
GGTCAGCGTC
AAGGTCGCCC
GGCGCGGCCG
GCGCTTCCGG
GGCGGGCGGG
TCCAGGACGT
CCTTGCCCCA
CACCAGGCCC
CAGGACTCGA
CGAGCCGCCG
GACGCCCGGT
AGGCCGTACG
TCTCGGCGAC
CTTGATGAGG
TCGAGGCGAC
GTCCGGCGAC
GCGGGCGATG
TATCGGTACC
AGATGTAGGC
CGGGATGACC
GCGATGGCGA
CCAGGCCCTC
GGTGTCGTCG
GTGATCTCCT
CCTCGGTGCG
GACGTCCTGC
TGGATGCCGA
GTTCCTTGAT
CAGCCGGTTC
AGGTTCTGCG
ACCGGTAGTG
CTTGCGGACC
TGGAAGACGC
CGAACTCGCG
CTCGCGGTAC
TTCTCGACGA
ACGGGCCGGG
CCGACGAAGC
CGCTGCAGCT
CGGCGGCCGC
CGCGTCGCCC
AGGTCGAGCG
GTCCCATGCG
GTCGTCGCCG
CGACCGGCCT
TGAAGTTCTG
TCCGGCCAGC
TCCAGGCCGA
TCTTGGCGAC
GCCGCCCTTG
GTCTTGTCGC
CGTCCTTGTA
GAGGTAGCGG
GCCTGCTTGC
CCGCATCGCC
GTCAGCGGCG
TCCGCGCCGT
TGAGTGGGCG
CACGTCGGTG
CCGTGGCCCT
TGCCCTCACA
GGAGCAACCG
GGCCGGTCGC
ACGTCTCGCT
GACGGTGTAG
CCGCCCGCGG
ATTCGACCCC
GGCGGCCCAG
GCTCCGGCGA
GTGCGTCGCG
GAACGCGGCC
TGGGCGTCCG
GGCCGAGCAC
CTCGCGGGTG
ACCCAGAGCG
TGTGCCAGTG
CAGGTGCCAG
CCGGAGCCCC
AGCCGAAGGT
GTCCTCGAAG
GCCCGCTCGT
AGCCGATGAT
CCCGAAGTCG
TCGCGCATCG
TGCGCCAGCG
GCGGCCGGAC
GAGCCGTACG
CGCCCTTCCA
GCCGTCGTGC
AAGACCGCGA
CCAGGCCGTG
CCGCATTCCC
TTGCGGACGG
TGCCGAACGC
CATGCGCTCG
AAGTGGCGCA
ACGTGTTCGT
GCCAAGGTGC
AGCCCGTACC
CGGCGTCCGC
GAGACCGTCG
GCGGCGAGCT
GCACGTTCGA
GCCCCGTACG
GCCAGGATGC
GGCTCATGCA
CCACGGGCAG
GTGTGGACGT
TGTTGCAGCG
GCACGTGTTG
CCCCACGTCG
CCTCGCCCGG
CTTCCACATC
AGCTCGGCCG
TCCCGGCAGT
GAGCCGGGTC
CCGCAGCCCT
TGAACGCCTC
GTTCAGCGAC
ACCGTCTGGT
GCCGGTCCCG
CCGGGCGAAC
CGCTCGTCGC
GCGGGTCCTC
CCGCCCGGCT
GTCGCGGCAC
CCTCGTTTGG
GGTAGAACCC
GTTCCAGTTA
CAGCGCTCTG
ACCTGCAGTG
GACGGAGATT
TTCCCTTACT
ACTAAAGCCC
GCGTCCGGAT
TACCCGCTGT
AGTCGTGCTT
GCTACGCTGC
GTGACTGGTC
CGCAATGAGA
CGCTTTGCGC
GCTTTCGGCA
GGCGTCCGAG
CAGTAGATTT
TGGGGCGCTT
CCCGGGGATG
TGGACGATCG
GGGTGCCGCA
GTGGCACTTC
GGTCCGGCGG
GGCGCGGTGG
TGTCGACGCG
CTGTTCTCTC
GTACGCTCGT
CACAGAGCAA
ACGTCCTCAC
TCGGCATGCT
GCGCCGGTTC
GGGGGCGGCG
AGCCCGGGAG
GCCAATCCCG
GGCTCGTGCC
ATTTCTGGGT
CCTGTTGATC
CTGGCATTGG
TGTGGCCGTT
CATTGCCCCT
GCTCGCTCCT
GACGCGCCGA
TAGACGTCCG
ATACGCCCGG
TGCTGGTGGG
ATTTGATAGG
TCGGAAGAAG
CCCCGCCCGG
GCCTGGGCGG
GGCTTCCTGT
GCGTCAGGAC
CTCCTCGTCG
TGAGCCTCTT
CGGCCTATGG
ACGGAGTGAC
CTCGTGATCC
GTTACAGCCG
CGCGCGCTCG
CGTAGAGCGG
TCTCATCAGT
TCCACGAACG
GTCCTCTTCG
CAGATCAGGG
CGTTGGGGCG
GAGTCTCACC
AAGGACTACG
TCTGCTGGCG
ATTTCCGTTA
CACCCCGGGC
GGTGGCCGGC
GCACACGCGC
GCCCGCGTTG
GGCAGTGCAG
AAAGTGCAGA
AACCTAGGCG
CTGATGGTCC
AGGTCCACGG
TTCGTCGTCG
GCGGCGGCGC
GGGCGGCGGC
GTCGGCCAGG
GCGCGGGCGA
GACCGGCTAC
GGCGGGCTTG
ATGCGCCGGT
TGCGGGCGAC
CTTGAGCAGC
TAGTATGCAG
GTCGACGGAT
CTTTTCCGCT
GCATAACCCT
GCTTCGGGGT
CATTATAGCG
ATTTTTTCGG
TATATCCATC
CTTTTTCGCA
CGATATACAG
GATTTTGCCA
AAGGGTTCGT
GTAGACTTTC
CTTGGTGTAT
CCAACGGCGT
CAGCCGGGCA
GGATAGGTGA
AGTAGGCCCA
CCCGCGAGCG
GGTGTTCCTT
CTTCACTGTC
CCTTATTCGC
ACCTGGCGGT
GCTCAACGGG
AATCCTGCTC
TGCGAGGCTG
GCCGGCTACC
GCCGGCGTAA
CAGATGAGGG
CAAGCGGATG
GCTGATGAAA
CCAAGCCAAC
CAGGAAGGGC
AGCCCACCTA
TCAAGGTGTA
CTGCCTTCCA
GACGAACGAA
GAGCGATTGA
GGAAAAGGCG
GCGGCGGCCG
GCATGAGCCT
GTCGGCCTAC
CTGCTGGCCG
TCGGCCAGGG
CTACAAAATC
ACGGGCGTCG
TGGACTATGA
GCACGTCCGC
GAGCTGGCCC
GCATCAATGG
CGACCTGGGC
CGCCTGGGCG
GCCTGCTGAA
ACTCTGGCTC
ACCGACGACC
CGCGCACGGC
GCGGTTCGGT
GATGCCACGA
TCCTCGCCCT
GCTGGCGAAG
ATCGAAGAGA
AGCAGGACGA
GCTTGGCAAG
GTCATGATGG
GCGTGGTCCG
CCCGAGGGCA
GAGCCATGAC
TTTTTTAGCC
GCTAAAACGG
CCGGGGGGTG
CGCGTGATTG
CCAAGCACGT
CCCCATGCGC
TCCATCAAGA
AGAGCGACTT
CGCGGAGCTG
GTGAAGTACA
TCACCGACGA
GCAAGGCAAG
ACCGATCCCC
GGGGACCTGC
ATAGATCTAC
GTCTGTCGAG
AAGTTTCTGA
TYGMMAAGYW
CGWCMSCRTS
CGCTCCATCA
AGAAGAGCGA
CTTCGCGGAG
CTGGTGAAGT
ACATCACCGA
CGAGCAAGGC
AAGACCGATC
CCCGGGGACC
TGCATAGATC
TACGTCTGTC
GAGAAGTTTC
TGATCGAAAA
GTTCGACAGC
GTCTCCGACC
TGATGCAGCT
CTCGGAGGGC
GAAGAATCTC
GTGCTTTCAG
CTTCGATGTA
GGAGGGCGTG
GATATGTCCT
GCGGGTAAAT
AGCTGCGCCG
ATGGTTTCTA
CAAAGATCGT
TATGTTTATC
GGCACTTTGC
ATCGGCCGCG
CTCCCGATTC
CGGAAGTGCT
TGACATTGGG
GAATTTATGC
GGTGTGAAAT
ACCGCACAGA
TGCGTAAGGA
GAAAATACCG
CATCAGGCGC
CATTCGCCAT
TCAGGCTGCG
CAACTGTTGG
GAAGGGCGAT
CGGTGCGGGC
CTCTTCGCTA
TTACGCCAGC
TGGCGAAAGG
GGGATGTGCT
GCAAGGCGAT
TAAGTTGGGT
AACGCCAGGG
TTTTCCCAGT
CACGACGTTG
TAAAACGACG
GCCAGTGCCA
AGCTTCGACC
GCCGCTCGAG
CCGGGCGCCC
AAAGCTGAGC
GCGTGAGGGA
CCACGAGGCA
GGGAGGGGAA
CACATGGCCA
CGCAGGACTC
CGAAGTCGCA
CTGGTGACGG
GTGCGACCAG
CGGAATCGGG
CTGGAGATCG
CCCGCAGGCT
CGGTAAGGAG
GGGTTGCGCG
TGTTCGTCTG
CGCGCGCGGC
GAAGAAGGAC
TGCGGACGAC
GCTGAAGGAG
TTGCGGGAGG
CGGGCGTGGA
GGCGGACGGG
CGGACCTGCG
ACGTCCGCTC
GGTCCCGGAG
ATCGAGGCTC
TCGTGGCGGC
GGTGGTCGAG
CGTTACGGTC
CGGTCGACGT
GCTGGTGAAC
AACGCGGGCC
GGCCCGGCGG
CGGCGCCACG
GCCGAACTCG
CCGACGAACT
CTGGCTCGAT
GTCGTGGAGA
CCAACCTCAC
CGGCGTGTTC
CGGGTGACCA
AACAGGTCCT
CAAGGCGGGC
GGCATGCTCG
AACGGGGCAC
GGGCCGAATC
GTCAACATCG
CCTCGACCGG
CGGAAAGCAG
GGTGTGGTGC
ACGCCGCGCC
CTACTCCGCC
TCGAAGCACG
GCGTGGTCGG
CTTCACCAAG
GCGCTCGGTC
TCGAACTGGC
GAGGACCGGC
ATCACGGTGA
ACGCCGTCTG
CCCCGGATTC
GTCGAGACGC
CGATGGCCGC
GTCCGTGCGC
GAGCACTACT
CGGACATCTG
GGAGGTGTCG
ACCGAGGAGG
CCTTCGACCG
GATCACCGCG
CGCGTGCCGA
TCGGCCGGTA
CGTGCAGCCG
TCCGAGGTGG
CGGAGATGGT
GGCGTACCTG
ATCGGGCCCG
GTGCGGCCGC
GTTCACGGAC
CGGTGCTGCG
CCCCCGTCGR
GRTYCWYCGK
CYSSTRSGKG
CRGCCGTCCG
AGGTGGCGGA
GATGGTGGCG
TACCTGATCG
GGCCCGGTGC
GGCCGCGTTC
ACGGACCGGT
GCTGCGCCCC
CGTCGAGATT
CTCCGTCTCC
TGGGGGCGAC
AATAACAGGC
CTACTCAACA
GATTTCAATG
TGCTTGCCGT
CACAAGCGAT
CTCCTATTGA
TCAAAAATTA
ATCAGTGCAG
CGAGCTGCAC
TGATTAATTT
TTGCTGGATT
TTACCGAGAG
GCAGTGGTCG
AGGCTTCCGA
ATCAATAGAA
TCTGGCATTC
CTGATACGGC
TGGGTCCTCG
GAAGGGTCCT
CGCGCCGGGC
GAGCGGGTGC
GGCCACCACA
TCCACCGGCC
GACATCGAGA
GTGAGCGCGG
TGACCAGGAC
CGATCGCACG
ATCATGGTGT
CGAGCAGGAC
GCCCACCGCG
ACCGTGAATC
CGAGCTCGGC
GATGAAGACC
AGCGGCAGCG
TGGCCAGCGC
GGCGAAGGTC
CCGGCCAGCA
CCAGCCCGGC
CGCGGTGATC
ACGCCACCGG
TCGAGGTGAG
GCCCTTGAGC
GCGCCCTGCC
GGGTGCCGAG
CCGGTCGCTC
TCCTCCTTGA
TCCGGGTGAC
GAGGAAGATG
TTGTAGTCGA
CGCCCAGGGC
GACCAGGAAG
ACGAAGACCC
ACAGGGGGAA
GGCCGAATCG
GCTCCCGCGA
AGTCGAAGAC
GTGGTTGAAG
AAGAGGGTGG
CGAGGCCGAG
CGCGGTGAAG
AAGGACAGCA
CCACGCTCGC
GATCAGCAGC
AGCGGCGCCA
CCAGTGCTCG
CAGCAGCAGC
GCGAGGATGC
AGAACACCAC
GGCGAGGATC
ACCGGAATGA
TCAGACCGCG
GTCGCGGCTG
GACGCCTCCC
GCATGTCGTG
CACGACGGCG
CTGCTGCCGC
CCACCCGTGC
CTGCGCACCG
TCCAGCCGGG
CGAGTGTCTC
GCGGGCCGCG
GTGACCGAAC
GCATGGCCGC
CGGCGAGTCC
GCCCCGGCGC
CGAGCGTCGC
CTCGACATAG
GCGAGGCCGT
CCTTCACCTG
TGGTGGTGCG
ACCTCGATGA
CGCCGGGCAC
GGTCTCCAGA
GCTGCGTGCA
CCTGGTCCGC
CGAGGCGCCC
TTGGCGATGA
CGACCATGGG
TTCACCGGAG
CCGGCCGGGA
AGTGCCGGGT
CAGGATCTCC
TCGCCCACCA
CCGACTCCGG
CTTGGTGGAA
TTCAGATCTC
AAAAAAAGCA
CCGACTCGGT
GCCACTTTTT
CAAGTTGATA
ACGGACTAGC
CTTATTTTAA
CTTGCTATTT
CTAGCTCTAA
AACGAACTCC
GGTCCCTGGT
AATACTAGTA
TTATACCTAG
GACTGAGCTA
GCTGTCAAGG
ATCCGCGGCC
GCGCGCGATC
TAGAGCAACC
GAGCGTTCTG
AACAAATCCA
GATGGAGTTC
TGAGGTCATT
ACTGGACCGG
ATCGGGGATC
TGGGCTGAGG
GAGCCGACGG
CACGCGGCGG
CTCACGGCGT
GGCACGCGGA
ACGTCCGGGC
TTGCACCTCA
CGTCACGTGA
GGAGGCAGCG
TGGACGGCGT
CAGAGAAGGG
AGCGGACATA
TGGACAAGAA
GTACTCCATC
GGCCTGGACA
TCGGCACCAA
CTCCGTCGGC
TGGGCCGTCA
TCACGGACGA
GTACAAGGTC
CCGTCGAAGA
AGTTCAAGGT
GCTGGGCAAC
ACGGACCGCC
ACAGCATCAA
GAAGAACCTC
ATCGGCGCCC
TCCTGTTCGA
CAGCGGCGAG
ACGGCCGAGG
CGACCCGCCT
GAAGCGCACG
GCGCGGCGGC
GGTACACGCG
CCGCAAGAAC
CGCATCTGCT
ACCTCCAGGA
GATCTTCTCC
AACGAGATGG
CGAAGGTCGA
CGACTCGTTC
TTCCACCGGC
TGGAGGAGTC
CTTCCTGGTC
GAGGAGGACA
AGAAGCACGA
GCGGCACCCC
ATCTTCGGCA
ACATCGTGGA
CGAGGTGGCC
TACCACGAGA
AGTACCCGAC
CATCTACCAC
CTCCGGAAGA
AGCTGGTGGA
CTCGACCGAC
AAGGCCGACC
TGCGCCTGAT
CTACCTGGCC
CTCGCCCACA
TGATCAAGTT
CCGCGGCCAC
TTCCTCATCG
AGGGCGACCT
GAACCCGGAC
AACTCGGACG
TCGACAAGCT
GTTCATCCAG
CTCGTGCAGA
CCTACAACCA
GCTCTTCGAG
GAGAACCCGA
TCAACGCCTC
CGGCGTGGAC
GCGAAGGCCA
TCCTGTCGGC
CCGCCTCTCG
AAGTCCCGGC
GGCTCGAGAA
CCTGATCGCC
CAGCTGCCGG
GCGAGAAGAA
GAACGGCCTC
TTCGGCAACC
TGATCGCGCT
CTCCCTGGGC
CTGACGCCGA
ACTTCAAGTC
CAACTTCGAC
CTCGCCGAGG
ACGCCAAGCT
CCAGCTGTCC
AAGGACACGT
ACGACGACGA
CCTGGACAAC
CTCCTGGCGC
AGATCGGCGA
CCAGTACGCG
GACCTGTTCC
TCGCGGCGAA
GAACCTCTCC
GACGCGATCC
TGCTCAGCGA
CATCCTCCGC
GTCAACACCG
AGATCACCAA
GGCGCCCCTG
TCCGCGAGCA
TGATCAAGCG
CTACGACGAG
CACCACCAGG
ACCTCACCCT
GCTGAAGGCC
CTGGTCCGCC
AGCAGCTCCC
GGAGAAGTAC
AAGGAGATCT
TCTTCGACCA
GTCCAAGAAC
GGCTACGCGG
GCTACATCGA
CGGCGGCGCG
TCCCAGGAGG
AGTTCTACAA
GTTCATCAAG
CCCATCCTGG
AGAAGATGGA
CGGCACCGAG
GAGCTGCTGG
TCAAGCTGAA
CCGGGAGGAC
CTCCTGCGCA
AGCAGCGCAC
GTTCGACAAC
GGCTCCATCC
CCCACCAGAT
CCACCTGGGC
GAGCTGCACG
CCATCCTCCG
GCGCCAGGAG
GACTTCTACC
CGTTCCTCAA
GGACAACCGC
GAGAAGATCG
AGAAGATCCT
CACGTTCCGG
ATCCCGTACT
ACGTCGGCCC
CCTCGCCCGC
GGCAACTCCC
GCTTCGCGTG
GATGACGCGC
AAGTCGGAGG
AGACGATCAC
CCCGTGGAAC
TTCGAGGAGG
TGGTGGACAA
GGGCGCCTCC
GCGCAGTCGT
TCATCGAGCG
GATGACCAAC
TTCGACAAGA
ACCTGCCGAA
CGAGAAGGTG
CTGCCCAAGC
ACAGCCTCCT
GTACGAGTAC
TTCACGGTCT
ACAACGAGCT
CACCAAGGTG
AAGTACGTGA
CGGAGGGCAT
GCGGAAGCCG
GCGTTCCTGT
CCGGCGAGCA
GAAGAAGGCC
ATCGTGGACC
TGCTGTTCAA
GACGAACCGG
AAGGTCACCG
TCAAGCAGCT
GAAGGAGGAC
TACTTCAAGA
AGATCGAGTG
CTTCGACTCG
GTCGAGATCA
GCGGCGTGGA
GGACCGCTTC
AACGCGTCGC
TGGGCACGTA
CCACGACCTC
CTGAAGATCA
TCAAGGACAA
GGACTTCCTC
GACAACGAGG
AGAACGAGGA
CATCCTGGAG
GACATCGTGC
TGACCCTGAC
GCTCTTCGAG
GACCGGGAGA
TGATCGAGGA
GCGCCTCAAG
ACCTACGCGC
ACCTGTTCGA
CGACAAGGTC
ATGAAGCAGC
TCAAGCGGCG
GCGGTACACC
GGCTGGGGCC
GGCTCTCCCG
CAAGCTCATC
AACGGCATCC
GGGACAAGCA
GTCGGGCAAG
ACCATCCTCG
ACTTCCTGAA
GTCCGACGGC
TTCGCCAACC
GCAACTTCAT
GCAGCTGATC
CACGACGACA
GCCTGACGTT
CAAGGAGGAC
ATCCAGAAGG
CGCAGGTGTC
GGGCCAGGGC
GACTCCCTGC
ACGAGCACAT
CGCGAACCTG
GCCGGCAGCC
CGGCCATCAA
GAAGGGCATC
CTGCAGACGG
TCAAGGTGGT
CGACGAGCTG
GTGAAGGTGA
TGGGCCGCCA
CAAGCCCGAG
AACATCGTCA
TCGAGATGGC
CCGGGAGAAC
CAGACCACGC
AGAAGGGCCA
GAAGAACAGC
CGCGAGCGCA
TGAAGCGGAT
CGAGGAGGGC
ATCAAGGAGC
TCGGCTCGCA
GATCCTCAAG
GAGCACCCCG
TCGAGAACAC
CCAGCTCCAG
AACGAGAAGC
TCTACCTCTA
CTACCTGCAG
AACGGCCGGG
ACATGTACGT
GGACCAGGAG
CTCGACATCA
ACCGGCTGTC
GGACTACGAC
GTCGACCACA
TCGTCCCGCA
GTCGTTCCTG
AAGGACGACA
GCATCGACAA
CAAGGTCCTC
ACCCGCTCGG
ACAAGAACCG
GGGCAAGTCC
GACAACGTCC
CGTCGGAGGA
GGTCGTGAAG
AAGATGAAGA
ACTACTGGCG
GCAGCTCCTC
AACGCCAAGC
TCATCACGCA
GCGCAAGTTC
GACAACCTGA
CGAAGGCGGA
GCGGGGCGGC
CTCAGCGAGC
TCGACAAGGC
GGGCTTCATC
AAGCGGCAGC
TGGTCGAGAC
GCGGCAGATC
ACGAAGCACG
TGGCCCAGAT
CCTGGACTCG
CGCATGAACA
CCAAGTACGA
CGAGAACGAC
AAGCTGATCC
GCGAGGTCAA
GGTCATCACC
CTCAAGTCCA
AGCTGGTGAG
CGACTTCCGC
AAGGACTTCC
AGTTCTACAA
GGTGCGGGAG
ATCAACAACT
ACCACCACGC
CCACGACGCG
TACCTGAACG
CGGTGGTGGG
CACGGCGCTG
ATCAAGAAGT
ACCCGAAGCT
CGAGAGCGAG
TTCGTCTACG
GCGACTACAA
GGTGTACGAC
GTGCGGAAGA
TGATCGCGAA
GAGCGAGCAG
GAGATCGGCA
AGGCCACGGC
GAAGTACTTC
TTCTACAGCA
ACATCATGAA
CTTCTTCAAG
ACCGAGATCA
CCCTGGCGAA
CGGCGAGATC
CGCAAGCGCC
CGCTCATCGA
GACCAACGGC
GAGACGGGCG
AGATCGTGTG
GGACAAGGGC
CGCGACTTCG
CGACCGTCCG
GAAGGTGCTG
TCCATGCCCC
AGGTCAACAT
CGTCAAGAAG
ACGGAGGTCC
AGACCGGCGG
CTTCAGCAAG
GAGTCCATCC
TGCCCAAGCG
GAACAGCGAC
AAGCTGATCG
CCCGGAAGAA
GGACTGGGAC
CCCAAGAAGT
ACGGCGGCTT
CGACTCCCCC
ACCGTCGCCT
ACTCGGTCCT
CGTGGTCGCC
AAGGTGGAGA
AGGGCAAGAG
CAAGAAGCTG
AAGTCCGTGA
AGGAGCTGCT
GGGCATCACC
ATCATGGAGC
GGTCGTCGTT
CGAGAAGAAC
CCCATCGACT
TCCTGGAGGC
CAAGGGCTAC
AAGGAGGTGA
AGAAGGACCT
CATCATCAAG
CTCCCCAAGT
ACTCCCTGTT
CGAGCTGGAG
AACGGCCGGA
AGCGCATGCT
CGCCAGCGCG
GGCGAGCTGC
AGAAGGGCAA
CGAGCTGGCG
CTGCCGAGCA
AGTACGTCAA
CTTCCTGTAC
CTCGCCTCGC
ACTACGAGAA
GCTGAAGGGC
TCGCCGGAGG
ACAACGAGCA
GAAGCAGCTG
TTCGTGGAGC
AGCACAAGCA
CTACCTCGAC
GAGATCATCG
AGCAGATCTC
CGAGTTCTCC
AAGCGCGTCA
TCCTGGCCGA
CGCGAACCTG
GACAAGGTCC
TGTCCGCGTA
CAACAAGCAC
CGCGACAAGC
CGATCCGGGA
GCAGGCCGAG
AACATCATCC
ACCTGTTCAC
GCTGACCAAC
CTCGGCGCCC
CCGCGGCCTT
CAAGTACTTC
GACACCACGA
TCGACCGGAA
GCGGTACACG
AGCACGAAGG
AGGTGCTCGA
CGCCACCCTG
ATCCACCAGA
GCATCACGGG
CCTGTACGAG
ACCCGCATCG
ACCTCTCCCA
GCTGGGCGGC
GACTGAGAAT
TCGTAATCAT
GTCATAGCTG
TTTCCTGTGT
GAAATTGTTA
TCCGCTCACA
ATTCCACACA
ACATACGAGC
CGGAAGCATA
AAGTGTAAAG
CCTGGGGTGC
CTAATGAGTG
AGCTAACTCA
CATTAATTGC
GTTGCGCTCA
CTGCCCGCTT
TCCAGTCGGG
AAACCTGTCG
TGCCAGCTGC
ATTAATGAAT
CGGCCAACGC
GCGGGGAGAG
GCGGTTTGCG
TATTGGGCGC
TCTTCCGCTT
CCTCGCTCAC
TGACTCGCTG
CGCTCGGTCG
TTCGGCTGCG
GCGAGCGGTA
TCAGCTCACT
CAAAGGCGGT
AATACGGTTA
TCCACAGAAT
CAGGGGATAA
CGCAGGAAAG
AACATGTGAG
CAAAAGGCCA
GCAAAAGGCC
AGGAACCGTA
AAAAGGCCGC
GTTGCTGGCG
TTTTTCCATA
GGCTCCGCCC
CCCTGACGAG
CATCACAAAA
ATCGACGCTC
AAGTCAGAGG
TGGCGAAACC
CGACAGGACT
ATAAAGATAC
CAGGCGTTTC
CCCCTGGAAG
CTCCCTCGTG
CGCTCTCCTG
TTCCGACCCT
GCCGCTTACC
GGATACCTGT
CCGCCTTTCT
CCCTTCGGGA
AGCGTGGCGC
TTTCTCATAG
CTCACGCTGT
AGGTATCTCA
GTTCGGTGTA
GGTCGTTCGC
TCCAAGCTGG
GCTGTGTGCA
CGAACCCCCC
GTTCAGCCCG
ACCGCTGCGC
CTTATCCGGT
AACTATCGTC
TTGAGTCCAA
CCCGGTAAGA
CACGACTTAT
CGCCACTGGC
AGCAGCCACT
GGTAACAGGA
TTAGCAGAGC
GAGGTATGTA
GGCGGTGCTA
CAGAGTTCTT
GAAGTGGTGG
CCTAACTACG
GCTACACTAG
AAGAACAGTA
TTTGGTATCT
GCGCTCTGCT
GAAGCCAGTT
ACCTTCGGAA
AAAGAGTTGG
TAGCTCTTGA
TCCGGCAAAC
AAACCACCGC
TGGTAGCGGT
GGTTTTTTTG
TTTGCAAGCA
GCAGATTACG
CGCAGAAAAA
AAGGATCTCA
AGAAGATCCT
TTGATCTTTT
CTACGGGGTC
TGACGCTCAG
TGGAACGAAA
ACTCACGTTA
AGGGATTTTG
GTCATGAGAT
TATCAAAAAG
GATCTTCACC
TAGATCCTTT
TGGTTCATGT
GCAGCTCCAT
CAGCAAAAGG
GGATGATAAG
TTTATCACCA
CCGACTATTT
GCAACAGTGC
CGTTGATCGT
GCTATGATCG
ACTGATGTCA
TCAGCGGTGG
AGTGCAATGT
CGTGCAATAC
GAATGGCGAA
AAGCCGAGCT
CATCGGTCAG
CTTCTCAACC
TTGGGGTTAC
CCCCGGCGGT
GTGCTGCTGG
TCCACAGCTC
CTTCCGTAGC
GTCCGGCCCC
TCGAAGATGG
GCCACTTGGA
CTGATCGAGG
CCCTGCGTGC
TGCGCTGGGT
CCGGGAGGGA
CGCTCGTCAT
GCCCTCGTGG
TCAGGTCTGG
ACGACGAGCC
GTTCGATCCT
GCCACGTCGC
CCGTTACACC
GGACCTTGGA
GTTGTCTCTG
ACACATTCTG
GCGCCTGCCA
AATGTAAAGC
GCAGCGCCCA
TCCATTTGCC
TTTGCGGCAG
CGGGGCCACA
GGCAGAGCAG
ATCATCTCTG
ATCCATTGCC
CCTGCCACCT
CACTCGCCTG
CAAGCCCGGT
CGCCCGTGTC
CATGAACTCG
ATGGGCAGGT
ACTTCTCCTC
GGCGTGGGAC
ACGATGCCAA
CACGACGCTG
CATCTTGCCG
AGTTGATGGC
AAAGGTTCCC
TATGGGGTGC
CGAGACACTG
CACCATTCTT
CAGGATGGCA
AGTTGGTACG
CGTCGATTAT
CTCGAGAATG
ACCACTGCTG
TGAGCGCTTT
GCCTTGGCGG
ACAGGTGGCT
CAAGGAGAAG
AGCCTTCAGA
AGGAAGGTCC
AGTCGGTCAT
GCCTTTGCTC
GGTTGATCCG
CTCCCGCGAC
ATTGTGGCGA
CAGCCCTGGG
TCAACTGGGC
CGAGATCCGT
TGATCTTCCT
GCATCCGCCA
GAGGCGGGAT
GCGAAGAATG
CGATGCCGCT
CGCCAGTCGA
TTGGCTGAGC
TCATGAGCGG
AGAACGAGAT
GACGTTGGAG
GGGCAAGGTC
GCGCTGATTG
CTGGGGCAAC
ACGTGGAGCG
GATCGGGGAT
TGTCTTTCTT
CAGCTCGCTG
ATGATATGCT
GACGCTCAAT
GCCGTTTGGC
CTCCGACTAA
CGAAAATCCC
GCATTTGGAC
GGCTGATCCG
ATTGGCACGG
CGGACGGCGA
ATGGCGGAGC
AGACGCTCGT
CCGGGGGCAA
TGAGATATGA
AAAAGCCTGA
ACTCACCGCG
ACGTATCGAT
GTCGAGGTTC
CTCAGGGGAG
CCACCCCAGA
GAAGCCCTCG
GAGCTGAGCG
GAGCTATTTC
CAAAGCCATG
CCAGCTAGAG
ACAGTGCACA
CCGCCAAGCA
TCTGCAAAAC
CCTCGCCTGG
AGGGAAAGTG
CAATGTACGT
ACGCTGGTTT
CCCTCCAGAA
AGGGATTCTG
CGGCTTCTCA
ACTTTGGAAG
AAGAGGCGGA
ACGAACTGCT
GTTTCAGCCT
ACTCA