名称: | pFC334 |
---|---|
别称: | Plasmid#87846 |
基因长: | |
质粒宿主: | 丝状真菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR,sgRNA |
片段物种: | |
原核抗性: | Amp |
筛选标记: | argB |
荧光标记: | |
启动子: | TEF1 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GCGCTGCGAT
GATACCGCGA
GAACCACGCT
CACCGGCTCC
GGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
CGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCGCTACAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAATACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
TTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTCA
GTGTTACAAC
CAATTAACCA
ATTCTGAACA
TTATCGCGAG
CCCATTTATA
CCTGAATATG
GCTCATAACA
CCCCTTGTTT
GCCTGGCGGC
AGTAGCGCGG
TGGTCCCACC
TGACCCCATG
CCGAACTCAG
AAGTGAAACG
CCGTAGCGCC
GATGGTAGTG
TGGGGACTCC
CCATGCGAGA
GTAGGGAACT
GCCAGGCATC
AAATAAAACG
AAAGGCTCAG
TCGAAAGACT
GGGCCTTTCG
CCCGGGCTAA
TTATGGGGTG
TCGCCCTTAT
TCGACTCTAT
AGTGAAGTTC
CTATTCTCTA
GAAAGTATAG
GAACTTCTGA
AGTGGGGATT
TAAATGCGGC
CGCGCTGAGG
GTTTAATCGA
CGAAGCAGCT
GACGGCCAGT
GCCAAGCTTA
ACGCGTACCG
GGCCCAGTAT
ATGTTCCGCA
GATGACTGGA
GCTCTGCCAT
ACGTGCCCTC
TCAAGCACCA
TTTGTTCCAT
CTACAGAGAC
TAGTCACCAA
CTAGTCTATC
AAGACTCACA
GGGTACATTG
CTGAGACCAA
CTGACCAGAG
GCAGGGTAGC
GGATTGACGG
CTCCATCTCC
TTCACTTACA
AGGTCTATTG
AAAGCCCTTT
AGCATCACCA
AGCGGAGAAT
AGATTGTTAA
GCTTATTTTT
TGTATACTGT
TTTGTGATAG
CACGAAGTTT
TTCCACGGTA
TCTTGTTAAA
AATATATATT
TGTGGCGGGC
TTACCTACAT
CAAATTAATA
AGAGACTAAT
TATAAACTAA
ACACACAAGC
AAGCTACTTT
AGGGTAAAAG
TTTATAAATG
CTTTTGACGT
ATAAACGTTG
CTTGTATTTA
TTATTACAAT
TAAAGGTGGA
TAGAAAACCT
AGAGACTAGT
TAGAAACTAA
TCTCAGGTTT
GCGTTAAACT
AAATCAGAGC
CCGAGAGGTT
AACAGAACCT
AGAAGGGGAC
TAGATATCCG
GGTAGGGAAA
CAAAAAAAAA
AANCAAGACA
GCCACATATT
AGGGAGACTA
GTTAGAAGCT
AGTTCCAGGA
CTAGGAAAAT
AAAAGACAAT
GATACCACAG
TCTAGTTGAC
AACTAGATAG
ATTCTAGATT
GAGGCCAAAG
TCTCTGAGAT
CCAGGTTAGT
TGCAACTAAT
ACTAGTTAGT
ATCTAGTCTC
CTATAACTCT
GAAGCTAGAA
TAACTTACTA
CTATTATCCT
CACCACTGTT
CAGCTGCGCA
AACGGAGTGA
TTGCAAGGTG
TTCAGAGACT
AGTTATTGAC
TAGTCAGTGA
CTAGCAATAA
CTAACAAGGT
ATTAACCTAC
CATGTCTGCC
ATCACCCTGC
ACTTCCTCGG
GCTCAGCAGC
CTTTTCCTCC
TCATTTTCAT
GCTCATTTTC
CTTGTTTAAG
ACTGTGACTA
GTCAAAGACT
AGTCCAGAAC
CACAAAGGAG
AAATGTCTTA
CCACTTTCTT
CATTGCTTGT
CTCTTTTGCA
TTATCCATGT
CTGCAACTAG
TTAGAGTCTA
GTTAGTGACT
AGTCCGACGA
GGACTTGCTT
GTCTCCGGAT
TGTTGGAGGA
ACTCTCCAGG
GCCTCAAGAT
CCACAACAGA
GCCTTCTAGA
AGACTGGTCA
ATAACTAGTT
GGTCTTTGTC
TGAGTCTGAC
TTACGAGGTT
GCATACTCGC
TCCCTTTGCC
TCGTCAATCG
ATGAGAAAAA
GCGCCAAAAC
TCGCAATATG
GCTTTGAACC
ACACGGTGCT
GAGACTAGTT
AGAATCTAGT
CCCAAACTAG
CTTGGATAGC
TTACCTTTGC
CCTTTGCGTT
GCGACAGGTC
TTGCAGGGTA
TGGTTCCTTT
CTCACCAGCT
GATTTAGCTG
CCTTGCTACC
CTCACGGCGG
ATCTGCATAA
AGAGTGGCTA
GAGGTTATAA
ATTAGCACTG
ATCCTAGGTA
CGGGGCTGAA
TGTAACTTGC
CYTTCCTTTC
TCATCGCGCG
GCAAGACAGG
CTTGCTCAAA
TTCCTACCAG
TCACAGGGGT
ATGCACGGCG
TACGGACCAC
TTGAACTAGT
CACAGATTAG
TTAGCAACTA
GTCTGCATTG
AATGGCTGTA
CTTACGGGCC
CTCGCCATTG
TCCTGATCAT
TTCCAGCTTC
ACCCTCGTTG
CTGCAAAGTA
GTTAGTGACT
AGTCAAGGAC
TAGTTGAAAT
GGGAGAAGAA
ACTCACGAAT
TCTCGACACC
CTTAGTATTG
TGGTCCTTGG
ACTTGGTGCT
GCTATATATT
AGCTAATACA
CTAGTTAGAC
TCACAGAAAC
TTACGCAGCT
CGCTTGCGCT
TCTTGGTAGG
AGTCGGGGTT
GGGAGAACAG
TGCCTTCAAA
CAAGCCTTCA
TACCATGCTA
CTTGACTAGT
CAGGGACTAG
TCACCAAGTA
ATCTAGATAG
GACTTGCCTT
TGGCCTCCAT
CAGTTCCTTC
ATAGTGGGAG
GTCCATTGTG
CAATGTAAAC
TCCATGCCGT
GGGAGTTCTT
GTCCTTCAAG
TGCTTGACCA
ATATGTTTCT
GTTGGCAGAG
GGAACCTGTC
AACTAGTTAA
TAACTAGTCA
GAAACTAGTA
TAGCAGTAGA
CTCACTGTAC
GCTTGAGGCA
TCCCTTCACT
CGGCAGTAGA
CTTCATATGG
ATGGATATCA
GGCACGCCAT
TGTCGTCCTG
TGGACTAGTC
AGTAACTAGG
CTTAAAGCTA
GTCGGGTCGG
CTTACTATCT
TGAAATCCGG
CAGCGTAAGC
TCCCCGTCCT
TAACTGCCTC
GAGATAGTGA
CAGTACTCTG
GGGACTTTCG
GAGATCGTTA
TCGCGAATGC
TCGGCATACT
AATCGTTGAC
TAGTCTTGGA
CTAGTCCCGA
GCAAAAAGGA
TTGGAGGAGG
AGGAGGAAGG
TGAGAGTGAG
ACAAAGAGCG
AAATAAGAGC
TTCAAAGGCT
ATCTCTAAGC
AGTATGAAGG
TTAAGTATCT
AGTTCTTGAC
TAGATTTAAA
AGAGATTTCG
ACTAGTTATG
TACCTGGAGT
TTGGATATAG
GAATGTGTTG
TGGTAACGAA
ATGTAAGGGA
GAGGAAAGAA
AAAGTCGGTC
AAGAGGTAAC
TCTAAGTCGG
CCATTCCTTT
TTGGGAGGCG
CTAACCATAA
ACGGCATGGT
CGACTTAGAG
TTAGCTCAGG
GAATTTAGGG
AGTTATCTGC
GACCACCGAG
GAACGGCGGA
ATGCCAAAGA
ATCCCGATGG
AGCTCTAGCT
GGCGGTTGAC
AACCCCACCT
TTTGGCGTTT
CTGCGGCGTT
GCAGGCGGGA
CTGGATACTT
CGTAGAACCA
GAAAGGCAAG
GCAGAACGCG
CTCAGCAAGA
GTGTTGGAAG
TGATAGCATG
ATGTGCCTTG
TTAACTAGGT
CAAAATCTGC
AGTATGCTTG
ATGTTATCCA
AAGTGTGAGA
GAGGAAGGTC
CAAACATACA
CGATTGGGAG
AGGGCCTAGG
TATAAGAGTT
TTTGAGTAGA
ACGCATGTGA
GCCCAGCCAT
CTCGAGGAGA
TTAAACACGG
GCCGGCATTT
GATGGCTATG
TTAGTACCCC
AATGGAAACG
GTGAGAGTCC
AGTGGTCGCA
GATAACTCCC
TAAATTCCCT
GAGCTAACTC
TAAGTCGACC
ATGCCGTTTA
TGGTTAGCGC
CTCCCAAAAA
GGAATGGCCG
ACTTAGAGTT
ACCTCTTGAC
CGACTTTTTC
TTTCCTCCCC
CTTACATTTC
GTTACCACAA
CACATTCCTA
TATCCAAACT
CCAGGTACAT
AACTAGTCGA
AATCTCTTTT
AAATCTAGTC
AAGAACTAGA
TACTTAACCT
TCATACTGCT
TAGAGATAGC
CTTTGAAGCT
CTTATTTCGC
TCTTTGTCTC
ACTCTCACCT
TCCTCCTCCT
CCTCCAATCC
TTTTTGCTCG
GGACTAGTCC
AAGACTAGTC
AACGATTAGT
ATGCCGAGCA
TTCGCGATAA
CGATCTCCGA
AAGTCCCCAG
AGTACTGTCA
CTATCTCGAG
GCAGTTAAGG
ACGGGGAGCT
TACGCTGCCG
GATTTCAAGA
TAGTAAGCCG
ACCCGACTAG
CTTTAAGCCT
AGTTACTGAC
TAGTCCACAG
GACGACAATG
GCGTGCCTGA
TATCCATCCA
TATGAAGTCT
ACTGCCGAGT
GAAGGGATGC
CTCAAGCGTA
CAGTGAGTCT
ACTGCTATAC
TAGTTTCTGA
CTAGTTATTA
ACTAGTTGAC
AGGTTCCCTC
TGCCAACAGA
AACATATTGG
TCAAGCACTT
GAAGGACAAG
AACTCCCACG
GCATGGAGTT
TACATTGCAC
AATGGACCTC
CCACTATGAA
GGAACTGATG
GAGGCCAAAG
GCAAGTCCTA
TCTAGATTAC
TTGGTGACTA
GTCCCTGACT
AGTCAAGTAG
CATGGTATGA
AGGCTTGTTT
GAAGGCACTG
TTCTCCCAAC
CCCGACTCCT
ACCAAGAAGC
GCAAGCGAGC
TGCGTAAGTT
TCTGTGAGTC
TAACTAGTGT
ATTAGCTAAT
ATATAGCAGC
ACCAAGTCCA
AGGACCACAA
TACTAAGGGT
GTCGAGAATT
CGTGAGTTTC
TTCTCCCATT
TCAACTAGTC
CTTGACTAGT
CACTAACTAC
TTTGCAGCAA
CGAGGGTGAA
GCTGGAAATG
ATCAGGACAA
TGGCGAGGGC
CCGTAAGTAC
AGCCATTCAA
TGCAGACTAG
TTGCTAACTA
ATCTGTGACT
AGTTCAAGTG
GTCCGTACGC
CGTGCATACC
CCTGTGACTG
GTAGGAATTT
GAGCAAGCCT
GTCTTGCCGC
GCGATGAGAA
AGGAARGGCA
AGTTACATTC
AGCCCCGTAC
CTAGGATCAG
TGCTAATTTA
TAACCTCTAG
CCACTCTTTA
TGCAGATCCG
CCGTGAGGGT
AGCAAGGCAG
CTAAATCAGC
TGGTGAGAAA
GGAACCATAC
CCTGCAAGAC
CTGTCGCAAC
GCAAAGGGCA
AAGGTAAGCT
ATCCAAGCTA
GTTTGGGACT
AGATTCTAAC
TAGTCTCAGC
ACCGTGTGGT
TCAAAGCCAT
ATTGCGAGTT
TTGGCGCTTT
TTCTCATCGA
TTGACGAGGC
AAAGGGAGCG
AGTATGCAAC
CTCGTAAGTC
AGACTCAGAC
AAAGACCAAC
TAGTTATTGA
CCAGTCTTCT
AGAAGGCTCT
GTTGTGGATC
TTGAGGCCCT
GGAGAGTTCC
TCCAACAATC
CGGAGACAAG
CAAGTCCTCG
TCGGACTAGT
CACTAACTAG
ACTCTAACTA
GTTGCAGACA
TGGATAATGC
AAAAGAGACA
AGCAATGAAG
AAAGTGGTAA
GACATTTCTC
CTTTGTGGTT
CTGGACTAGT
CTTTGACTAG
TCACAGTCTT
AAACAAGGAA
AATGAGCATG
AAAATGAGGA
GGAAAAGGCT
GCTGAGCCCG
AGGAAGTGCA
GGGTGATGGC
AGACATGGTA
GGTTAATACC
TTGTTAGTTA
TTGCTAGTCA
CTGACTAGTC
AATAACTAGT
CTCTGAACAC
CTTGCAATCA
CTCCGTTTGC
GCAGCTGAAC
AGTGGTGAGG
ATAATAGTAG
TAAGTTATTC
TAGCTTCAGA
GTTATAGGAG
ACTAGATACT
AACTAGTATT
AGTTGCAACT
AACCTGGATC
TCAGAGACTT
TGGCCTCAAT
CTAGAATCTA
TCTAGTTGTC
AACTAGACTG
TGGTATCATT
GTCTTTTATT
TTCCTAGTCC
TGGAACTAGC
TTCTAACTAG
TCTCCCTAAT
ATGTGGCTGT
CTTGTTTTTT
TTTTTTGTTT
CCCTACCCGG
ATATCTAGTC
CCCTTCTAGG
TTCTGTTAAC
CTCTCGGGCT
CTGATTTAGT
TTAACGCAAA
CCTGAGATTA
GTTTCTAACT
AGTCTCTAGG
TTTTCTATCC
ACCTTTAATT
GTAATAATAA
ATACAAGCAA
CGTTTATACG
TCAAAAGCAT
TTATAAACTT
TTACCCTAAA
GTAGCTTGCT
TGTGTGTTTA
GTTTATAATT
AGTCTCTTAT
TAATTTGATG
TAGGTAAGCC
CGCCACAAAT
ATATATTTTT
AACAAGATAC
CGTGGAAAAA
CTTCGTGCTA
TCACAAAACA
GTATACAAAA
AATAAGCTTA
ACAATCTATT
CTCCGCTTGG
TGATGCTAAA
GGGCTTTCAA
TAGACCTTGT
AAGTGAAGGA
GATGGAGCCG
TCAATCCGCT
ACCCTGCCTC
TGGTCAGTTG
GTCTCAGCAA
TGTACCCTGT
GAGTCTTGAT
AGACTAGTTG
GTGACTAGTC
TCTGTAGATG
GAACAAATGG
TGCTTGAGAG
GGCACGTATG
GCAGAGCTCC
AGTCATCTGC
GGAACATATA
CTGGGCCCGG
GAAGATCTCA
TGGTCATAGC
TGTTTCCGCT
GAGGGTTTAA
TGCGTAAGCT
CCCTAATTGG
CCCATCCGGC
ATCTGTAGGG
CGTCCAAATA
TCGTGCCTCT
CCTGCTTTGC
CCGGTGTATG
AAACCGGAAA
GGCCGCTCAG
GAGCTGGCCA
GCGGCGCAGA
CCGGGAACAC
AAGCTGGCAG
TCGACCCATC
CGGTGCTCTA
CACTCGACCT
GCTGAGGTCC
CTCAGTCCCT
GGTAGGCAGC
TTTGCCCCGT
CTGTCCGCCC
GGTGTGTCGG
CGGGGTTGAC
AAGGTCGTTG
CGTCAGTCCA
ACATTTGTTG
CCATATTTTC
CTGCTCTCCC
CACCAGCTGC
TCTTTTCTTT
TCTCTTTCTT
TTCCCATCTT
CAGTATATTC
ATCTTCCCAT
CCAAGAACCT
TTATTTCCCC
TAAGTAAGTA
CTTTGCTACA
TCCATACTCC
ATCCTTCCCA
TCCCTTATTC
CTTTGAACCT
TTCAGTTCGA
GCTTTCCCAC
TTCATCGCAG
CTTGACTAAC
AGCTACCCCG
CTTGAGCAGA
CATCACCGTC
CGCCCTGATG
AGTCCGTGAG
GACGAAACGA
GTAAGCTCGT
CGGCGGAGTA
TCATAACATC
GGTTTTAGAG
CTAGAAATAG
CAAGTTAAAA
TAAGGCTAGT
CCGTTATCAA
CTTGAAAAAG
TGGCACCGAG
TCGGTGCTTT
TGGCCGGCAT
GGTCCCAGCC
TCCTCGCTGG
CGCCGGCTGG
GCAACATGCT
TCGGCATGGC
GAATGGGACT
GATTTAATAG
CTCCATGTCA
ACAAGAATAA
AACGCGTTTC
GGGTTTACCT
CTTCCAGATA
CAGCTCATCT
GCAATGCATT
AATGCATTGG
ACCTCGCAAC
CCTAGTACGC
CCTTCAGGCT
CCGGCGAAGC
AGAAGAATAG
CTTAGCAGAG
TCTATTTTCA
TTTTCGGGAG
ACGAGATCAA
GCAGATCAAC
GGTCGTCAAG
AGACCTACGA
GACTGAGGAA
TCCGCTCTTG
GCTCATTAAG
ACCTCAGCCG
AGACAGCAGA
ATCACCGCCC
AAGTTAAGCC
TTTGTGCTGA
TCATGCTCTC
GAACGGGCCA
AGTTCGGGAA
AAGCAAAGGA
GCGTTTAGTG
AGGGGCAATT
TGACTCACCT
CCCAGGCAAC
AGATGAGGGG
GGCAAAAAGA
AAGAAATTTT
CGTGAGTCAA
TATGGATTCC
GAGCATCATT
TTCTTGCGGT
CTATCTTGCT
ACGTATGTTG
ATCTTGACGC
TGTGGATCAA
GCAACGCCAC
TCGCTCGCTC
CATCGCAGGC
TGGTCGCAGA
CAAATTAAAA
GGCGGCAAAC
TCGTACAGCC
GCGGGGTTGT
CCGCTGCAAA
GTACAGAGTG
ATAAAAGCCG
CCATGCGACC
ATCAACGCGT
TGATGCCCAG
CTTTTTCGAT
CCGAGAATCC
ACCGTAGAGG
CGATAGCAAG
TAAAGAAAAG
CTAAACAAAA
AAAAATTTCT
GCCCCTAAGC
CATGAAAACG
AGATGGGGTG
GAGCAGAACC
AAGGAAAGAG
TCGCGCTGGG
CTGCCGTTCC
GGAAGGTGTT
GTAAAGGCTC
GACGCCCAAG
GTGGGAGTCT
AGGAGAAGAA
TTTGCATCGG
GAGTGGGGCG
GGTTACCCCT
CCATATCCAA
TGACAGATAT
CTACCAGCCA
AGGGTTTGAG
CCCGCCCGCT
TAGTCGTCGT
CCTCGCTTGC
CCCTCCATAA
AAGGATTTCC
CCTCCCCCTC
CCACAAAATT
TTCTTTCCCT
TCCTCTCCTT
GTCCGCTTCA
GTACGTATAT
CTTCCCTTCC
CTCGCTTCTC
TCCTCCATCC
TTCTTTCATC
CATCTCCTGC
TAACTTCTCT
GCTCAGCACC
TCTACGCATT
ACTAGCCGTA
GTATCTGAGC
ACTTCTCCCT
TTTATATTCC
ACAAAACATA
ACACAACCTT
CACCATGGAC
AAGAAGTATA
GCATCGGGCT
GGACATTGGA
ACGAACTCGG
TTGGTTGGGC
TGTGATTACG
GACGAATACA
AGGTGCCATC
CAAGAAGTTT
AAGGTCCTGG
GAAACACCGA
CCGTCACTCA
ATCAAGAAGA
ATCTCATTGG
AGCCCTGCTC
TTCGATAGTG
GGGAGACCGC
CGAAGCTACT
CGACTGAAGC
GAACGGCTCG
CCGGCGTTAT
ACACGACGCA
AGAATCGCAT
CTGCTACCTC
CAGGAGATTT
TCAGCAACGA
AATGGCTAAG
GTTGATGACT
CATTCTTTCA
TCGACTCGAA
GAAAGTTTCT
TGGTCGAGGA
GGATAAGAAG
CACGAGCGCC
ATCCGATCTT
TGGTAACATT
GTGGATGAGG
TTGCCTATCA
CGAAAAGTAC
CCAACTATCT
ATCATCTTCG
TAAGAAGCTG
GTCGATAGCA
CGGACAAGGC
TGATTTGCGA
CTTATCTACC
TGGCACTCGC
GCACATGATT
AAGTTCCGCG
GCCATTTTCT
TATCGAGGGT
GACCTGAACC
CCGATAATTC
TGACGTTGAT
AAGCTCTTCA
TCCAGTTGGT
CCAAACCTAC
AATCAGCTGT
TTGAGGAAAA
CCCTATTAAT
GCATCTGGCG
TGGACGCCAA
GGCTATCCTT
TCGGCGCGCC
TGTCTAAGTC
GCGGCGTTTG
GAGAACCTTA
TCGCACAACT
CCCCGGCGAA
AAGAAGAACG
GCCTCTTCGG
TAATTTGATT
GCGTTGTCAC
TTGGTCTGAC
TCCTAACTTC
AAGAGTAATT
TTGACCTGGC
AGAGGATGCG
AAGCTCCAGT
TGTCTAAGGA
TACGTATGAT
GACGATCTCG
ACAACTTGCT
TGCCCAAATC
GGTGACCAGT
ACGCTGATCT
TTTCCTGGCC
GCTAAGAATC
TCTCAGATGC
AATCCTGCTC
AGTGACATTT
TGCGGGTCAA
CACCGAGATT
ACTAAGGCCC
CCCTGTCAGC
TAGTATGATC
AAGCGGTATG
ATGAGCACCA
TCAGGACCTC
ACCTTGCTTA
AGGCCCTCGT
GCGTCAGCAA
TTGCCTGAGA
AGTATAAGGA
AATCTTCTTT
GACCAATCCA
AGAACGGATA
CGCAGGGTAT
ATTGATGGCG
GTGCGAGCCA
GGAGGAATTC
TACAAGTTTA
TCAAGCCGAT
TTTGGAGAAG
ATGGACGGCA
CTGAGGAACT
GCTCGTCAAG
CTGAATCGCG
AAGATTTGCT
TCGTAAGCAA
CGAACGTTCG
ACAACGGCTC
CATCCCGCAC
CAGATTCATC
TGGGCGAGCT
CCACGCCATC
CTTCGACGCC
AGGAAGATTT
CTACCCATTT
CTGAAGGACA
ACCGTGAGAA
GATCGAAAAG
ATTCTTACAT
TCCGAATCCC
CTACTATGTG
GGACCTTTGG
CCCGTGGGAA
TTCCCGATTT
GCTTGGATGA
CCCGAAAGAG
CGAGGAAACC
ATCACTCCGT
GGAACTTCGA
GGAAGTCGTG
GACAAGGGTG
CATCCGCGCA
GAGCTTCATT
GAGCGGATGA
CCAATTTTGA
TAAGAACCTT
CCGAATGAAA
AGGTCCTGCC
AAAGCATTCG
CTGCTCTACG
AGTATTTCAC
CGTGTATAAC
GAACTGACTA
AGGTCAAGTA
CGTGACGGAG
GGAATGCGGA
AGCCAGCCTT
CCTCTCAGGG
GAACAAAAGA
AGGCTATCGT
CGATTTGCTT
TTTAAGACCA
ATCGTAAAGT
GACTGTTAAG
CAGCTGAAGG
AGGATTATTT
CAAGAAGATT
GAATGTTTCG
ACTCCGTCGA
GATCAGCGGC
GTGGAAGATC
GCTTTAACGC
TTCCCTCGGT
ACCTACCACG
ACCTGCTCAA
GATCATTAAG
GACAAGGATT
TCCTCGATAA
CGAGGAAAAT
GAGGACATCT
TGGAAGATAT
TGTCCTCACG
TTGACACTTT
TTGAGGACCG
CGAAATGATC
GAGGAACGGC
TCAAGACATA
TGCCCATTTG
TTCGACGATA
AGGTGATGAA
GCAGCTGAAG
CGGCGTCGAT
ACACCGGATG
GGGTCGCCTT
AGCCGGAAGC
TGATCAACGG
CATTCGAGAT
AAGCAATCTG
GTAAGACTAT
CTTGGATTTC
CTTAAGTCGG
ACGGCTTCGC
CAACCGCAAT
TTTATGCAGC
TTATTCACGA
CGATTCCCTG
ACGTTCAAGG
AGGACATCCA
GAAGGCACAA
GTCTCAGGAC
AAGGGGATTC
CCTGCACGAG
CATATCGCCA
ACCTGGCTGG
ATCCCCGGCG
ATCAAGAAGG
GGATTCTTCA
GACCGTCAAG
GTTGTCGACG
AGCTGGTCAA
GGTGATGGGC
CGTCATAAGC
CAGAAAACAT
CGTGATTGAG
ATGGCCCGAG
AAAATCAGAC
CACTCAAAAG
GGTCAGAAGA
ACAGCCGCGA
GCGGATGAAG
CGGATCGAGG
AAGGCATTAA
GGAACTTGGT
TCTCAGATCC
TGAAGGAGCA
CCCTGTTGAA
AACACACAGC
TCCAAAATGA
GAAGCTGTAT
CTCTACTATT
TGCAAAATGG
ACGCGACATG
TACGTCGATC
AGGAGCTCGA
CATTAACCGG
TTGTCGGACT
ACGATGTTGA
CCATATCGTC
CCGCAATCCT
TCCTTAAGGA
CGATAGCATT
GATAACAAGG
TGCTGACTCG
CTCAGATAAG
AACCGGGGCA
AGTCCGACAA
TGTTCCAAGC
GAGGAAGTGG
TTAAGAAGAT
GAAGAACTAC
TGGCGCCAAT
TGCTTAATGC
CAAGCTCATC
ACACAGCGCA
AGTTTGACAA
CTTGACCAAG
GCCGAGCGGG
GAGGGCTGAG
TGAACTCGAT
AAGGCTGGCT
TCATCAAGCG
TCAACTCGTG
GAGACGCGAC
AGATCACAAA
GCACGTTGCT
CAGATTCTGG
ACTCCCGGAT
GAACACAAAG
TACGACGAGA
ATGATAAGCT
CATCCGTGAA
GTTAAGGTCA
TTACCCTCAA
GTCTAAGTTG
GTGTCGGATT
TCCGCAAGGA
CTTCCAATTT
TATAAGGTTC
GGGAGATCAA
CAATTATCAC
CATGCACATG
ATGCGTACCT
CAACGCAGTC
GTGGGAACTG
CGCTCATCAA
GAAGTATCCC
AAGTTGGAGT
CCGAATTCGT
CTACGGGGAT
TATAAGGTTT
ACGACGTCCG
CAAGATGATC
GCCAAGAGTG
AGCAGGAAAT
TGGCAAGGCC
ACGGCTAAGT
ATTTCTTTTA
CTCCAACATC
ATGAATTTCT
TTAAGACGGA
GATCACACTC
GCCAATGGAG
AAATCCGTAA
GCGACCTTTG
ATTGAGACCA
ACGGCGAGAC
TGGTGAAATC
GTTTGGGATA
AGGGGCGCGA
CTTCGCTACC
GTGCGGAAGG
TTCTGAGCAT
GCCGCAAGTC
AATATCGTCA
AGAAAACCGA
GGTGCAGACA
GGCGGTTTCT
CTAAGGAATC
GATTCTTCCA
AAGCGTAACT
CTGACAAGCT
GATCGCTCGA
AAGAAGGATT
GGGACCCCAA
GAAGTATGGA
GGGTTCGATT
CTCCTACAGT
GGCATACTCG
GTTCTCGTTG
TCGCGAAGGT
TGAGAAGGGA
AAGTCTAAGA
AGCTGAAGTC
GGTCAAGGAA
CTGCTCGGGA
TCACCATTAT
GGAGCGCTCC
AGCTTCGAAA
AGAATCCCAT
CGACTTTCTC
GAGGCCAAGG
GCTATAAGGA
AGTCAAGAAG
GATCTTATCA
TTAAGCTGCC
TAAGTACTCT
TTGTTCGAGC
TTGAAAACGG
TCGAAAGCGA
ATGCTCGCAT
CGGCAGGAGA
GTTGCAGAAG
GGGAATGAAT
TGGCACTTCC
CTCAAAGTAC
GTGAACTTCC
TGTATCTCGC
GTCCCACTAC
GAGAAGCTGA
AGGGTAGCCC
TGAGGACAAC
GAACAGAAGC
AACTTTTTGT
TGAGCAACAC
AAGCATTATC
TGGATGAGAT
CATTGAACAG
ATTTCAGAGT
TCAGTAAGCG
CGTCATCCTC
GCCGATGCTA
ATCTCGACAA
GGTGTTGTCG
GCCTACAACA
AGCACCGTGA
CAAGCCGATC
CGAGAGCAGG
CTGAAAATAT
CATTCATCTG
TTCACCCTCA
CTAACTTGGG
AGCACCAGCA
GCGTTCAAGT
ATTTTGATAC
GACAATCGAC
CGTAAGCGAT
ACACGTCCAC
AAAGGAGGTG
CTTGATGCGA
CCCTGATTCA
TCAATCCATC
ACTGGGCTCT
ATGAAACCCG
TATCGACCTT
AGTCAACTGG
GGGGCGACCC
TCCCAAGAAG
AAGCGCAAGG
TCTGAGCGGA
CATTCGATTT
ATGCCGTTAT
GACTTCCTTA
AAAAAGCCTT
TACGAATGAA
AGAAATGGAA
TTAGACTTGT
TATGTAGTTG
ATTCTACAAT
GGATTATGAT
TCCTGAACTT
CAAATCCGCT
GTTCATTATT
AATCTCAGCT
CTTCCCGTAA
AGCCAATGTT
GAAACTATTC
GTAAATGTAC
CTCGTTTTGC
GTGTACCTTG
CTTATCACGT
GATATTACAT
GACCTGGACA
GAGTTCTGCG
CGAAAGTCAT
AACGTAAATC
CCGGGCGGTA
GGTGCGTCCC
GGGCGGAAGG
TAGTTTTCTC
GTCCACCCCA
ACGCGTTTAT
CAACCTCAAC
TTTCAACAAC
CATCATGCCA
CCAAAAGCGC
GTAAAACAAA
GCGAGATTTG
ATTGAGCAAG
AGGGCAGGAT
CCAATGCGCG
ATTCAAGACA
TTAAAAATGG
AAAATTTCAA
AAAATTGCGC
CCGCAGCGCG
TGCATACAAA
ATTCATCCCA
ATACAAGTGT
AATACTAGGT
AATATCGCGT
GCATTCCGCG
GTTTTTTGGG
GTAGTCATCT
AATGAAACAG
ACCCGGACGC
AGCAGAGGAA
GCCCGCGATG
ACTCTATACC
ACCGTACGCC
GATATATCAT
CATCGCGGCG
ATGGAGAAGT
TGGGTTGACT
CCGAAGACAC
TTCAAAGGAG
CGACGCTGTT
GATTTGTAGA
CGACGCTTGA
TAGGGAGAAG
CATTATTGTC
GTGATGCTCG
CCCAACAGAG
GCCGACTCGC
CTCATCCGTC
ATAACGAACG
CTGTGTAAAG
CGGAGTGGGG
GGGAAAGTGT
GGATTGTGGA
GAGTATGCGA
TAGTGTTGAG
GCTGATCAGA
CGGCGAATCG
GGCCAGATAT
GACCAGTTTA
GAGGCCTCAT
TTGACTATAA
TTTACATAAA
TTAGATAAAT
AGAGATGAAC
GCATGCAATA
ATTGCAGCAA
ATATTGATGA
AGCGAGAGGT
AGGACGATGA
AGGACTGTGA
GCAGTTCAAG
GTATCAGCAG
AGTCAAGGGC
CTGATGCAAT
GGCGGTGATC
CGTGATCAGC
GAACGGAAGG
GGCGCTAACT
CTGTTTCTTT
ACCAATGATC
GGAAGCTCCT
GCTGGCGGAC
TTATGAGTCA
TTCACGAATC
ATTTCTCAGT
TATTTGTGGA
TGCCCTCGTT
CTGTCCACAA
TTTCTTTCCG
CCCCAAGTCT
TTTAAGTTCT
TTAACATCTA
TATTCTTGCA
CTTCCAATGG
CATCCCTTCG
CTCCGTACTC
AAGAGCCAGA
GCTTGCGACA
CACCGTGCGA
TCCTACTCCT
CGCAAACCAT
GCCTCCCGCC
TCACCCTTTG
CTCCCCGCCA
CTTCCTCTCC
ATTGCGGACC
TCTCGCCCTC
CGAGTTCGCA
ACCCTTGTTC
GCAATGCCTC
CTCACACAAA
CGGGCTATCA
AGTCGGGGTC
AATGCCCCAA
AACTTGCAGG
GATCACTCCT
TGGGAAAACT
GTGGCCATGA
TCTTCAGCAA
ACGAAGCACG
AGGACAAGGG
TATCTACAGA
AGGGGCCGTT
GTGCAGATGG
GAGGTCATCC
GATGTTCTTG
GGCAAGGATG
ATATCCAACT
AGGTGTCAAC
GAGTCCCTAT
ACGACACCTC
CGTTGTCATT
TCGTCCATGG
TATCCTGCAT
TGTAGCCCGT
GTCGGTAAAC
ATGCAGAGGT
CGCAGATCTG
GCGAAGCACT
CTTCGGTTCC
AGTCATCAAT
GCTTTGTGTG
ACTCTTTCCA
CCCTCTCCAA
GCCGTGGCCG
ATTTCCAGAC
CATCTATGAA
GCATTCACCC
CCAAGGCGCA
CCACCTTTCA
AGTCTAGGGT
TGGAAGGATT
GAAGATCGCT
TGGGTGGGTG
ACGCCAACAA
CGTCCTGTTC
GATATGGCCA
TTGCTGCTAC
AAAAATGGGT
GTCGACATTG
CTGTCGCTAC
TCCCAAGGGG
TACGAAATCC
CTCCTCACAT
GCTGGAGCTC
ATCAAGTCTG
CTGGAGAGGG
TGTCTCGAAA
CCAGGAAAGC
TTCTGCAAAC
CAATATTCCC
GAAGAAGCGG
TCAAGGACGC
CGATATTCTG
GTCACAGACA
CCTGGGTCTC
TATGGGCCAA
GAGGAAGAGA
AGGCTCAGAG
GCTGAAGGAG
TTTGATGGTT
TCCAAATCAC
TGCTGAACTC
GCCAAGCGAG
GAGGAGCTAA
GGAGGGCTGG
AAGTTCATGC
ACTGTCTCCC
GCGACACCCT
GAGGAGGTCA
GCGACGAGGT
TTTCTACAGC
AACCGGTCAC
TTGTCTTCCC
TGAGGCTGAG
AACCGGTTAT
GGGCTGCGAT
TTCCGCCTTG
GAGGGTTTCG
TTGTCAATAA
GGGAAAAATT
GAATAAATAT
AACCAGGCTT
CCATTTAAAT
ATATAGAGGC
TGGCGTTATC
AAACTGATGA
GTTGACGGGT
ATGAGATCAT
TCCGTCCCTA
AATATATTTA
CTCCGATCAC
GTAAAAGCCT
GTTAGTAGAA
GCATTTTCCC
AATTATCCTG
ACCAATTCTT
TCTAGCATAT
ATCAAATAAC
TAATTGACAT
GTTCTCTCGC
TTCCTTATAT
TACTCAGAGT
ATTGGAAATG
GGGCAAATCG
CACCCGGTGA
CTTTCACATG
TCACGAATGC
GGAGTCGTCC
TAGCCAAGGT
AGATCCAGGC
CTAACACACC
CCAACCCTCG
ACTCTCCTCA
TTCCATACTC
TTGACCTCTA
TCCAGCACAT
TCTTCTGAGG
TTTCGCAATG
GCTGTAGGTG
GCGCTGAGGC
ATCCCCACTA
CCGCATTAAG
ACCTCAGCGC
GGCCGCAAAT
TTAAATAAAA
TGAAGTGAAG
TTCCTATACT
TTCTAGAGAA
TAGGAACTTC
TATAGTGAGT
CGAATAAGGG
CGACACAAAA
TTTATTCTAA
ATGCATAATA
AATACTGATA
ACATCTTATA
GTTTGTATTA
TATTTTGTAT
TATCGTTGAC
ATGTATAATT
TTGATATCAA
AAACTGATTT
TCCCTTTATT
ATTTTCGAGA
TTTATTTTCT
TAATTCTCTT
TAACAAACTA
GAAATATTGT
ATATACAAAA
AATCATAAAT
AATAGATGAA
TAGTTTAATT
ATAGGTGTTC
ATCAATCGAA
AAAGCAACGT
ATCTTATTTA
AAGTGCGTTG
CTTTTTTCTC
ATTTATAAGG
TTAAATAATT
CTCATATATC
AAGCAAAGTG
ACAGGCGCCC
TTAAATATTC
TGACAAATGC
TCTTTCCCTA
AACTCCCCCC
ATAAAAAAAC
CCGCCGAAGC
GGGTTTTTAC
GTTATTTGCG
GATTAACGAT
TACTCGTTAT
CAGAACCGCC
CAGGGGGCCC
GAGCTTAAGA
CTGGCCGTCG
TTTTACAACA
CAGAAAGAGT
TTGTAGAAAC
GCAAAAAGGC
CATCCGTCAG
GGGCCTTCTG
CTTAGTTTGA
TGCCTGGCAG
TTCCCTACTC
TCGCCTTCCG
CTTCCTCGCT
CACTGACTCG
CTGCGCTCGG
TCGTTCGGCT
GCGGCGAGCG
GTATCAGCTC
ACTCAAAGGC
GGTAATACGG
TTATCCACAG
AATCAGGGGA
TAACGCAGGA
AAGAACATGT
GAGCAAAAGG
CCAGCAAAAG
GCCAGGAACC
GTAAAAAGGC
CGCGTTGCTG
GCGTTTTTCC
ATAGGCTCCG
CCCCCCTGAC
GAGCATCACA
AAAATCGACG
CTCAAGTCAG
AGGTGGCGAA
ACCCGACAGG
ACTATAAAGA
TACCAGGCGT
TTCCCCCTGG
AAGCTCCCTC
GTGCGCTCTC
CTGTTCCGAC
CCTGCCGCTT
ACCGGATACC
TGTCCGCCTT
TCTCCCTTCG
GGAAGCGTGG
CGCTTTCTCA
TAGCTCACGC
TGTAGGTATC
TCAGTTCGGT
GTAGGTCGTT
CGCTCCAAGC
TGGGCTGTGT
GCACGAACCC
CCCGTTCAGC
CCGACCGCTG
CGCCTTATCC
GGTAACTATC
GTCTTGAGTC
CAACCCGGTA
AGACACGACT
TATCGCCACT
GGCAGCAGCC
ACTGGTAACA
GGATTAGCAG
AGCGAGGTAT
GTAGGCGGTG
CTACAGAGTT
CTTGAAGTGG
TGGGCTAACT
ACGGCTACAC
TAGAAGAACA
GTATTTGGTA
TCTGCGCTCT
GCTGAAGCCA
GTTACCTTCG
GAAAAAGAGT
TGGTAGCTCT
TGATCCGGCA
AACAAACCAC
CGCTGGTAGC
GGTGGTTTTT
TTGTTTGCAA
GCAGCAGATT
ACGCGCAGAA
AAAAAGGATC
TCAAGAAGAT
CCTTTGATCT
TTTCTACGGG
GTCTGACGCT
CAGTGGAACG
ACGCGCGCGT
AACTCACGTT
AAGGGATTTT
GGTCATGAGC
TTGCGCCGTC
CCGTCAAGTC
AGCGTAATGC
TCTGCTT