产品货号：P13307
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | hRad51(A89E)–Cas9(D10A) |
|---|---|
| 别称: | Plasmid #125563 |
| 基因长: | |
| 质粒宿主： | 哺乳细胞 |
| 质粒用途： | 基因编辑 |
| 片段类型： | CRISPR |
| 片段物种： | 人 |
| 原核抗性： | Amp |
| 筛选标记： | |
| 荧光标记： | |
| 启动子: | CMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | |
| 正向引物: | |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
ATATGCCAAG
TACGCCCCCT
ATTGACGTCA
ATGACGGTAA
ATGGCCCGCC
TGGCATTATG
CCCAGTACAT
GACCTTATGG
GACTTTCCTA
CTTGGCAGTA
CATCTACGTA
TTAGTCATCG
CTATTACCAT
GGTGATGCGG
TTTTGGCAGT
ACATCAATGG
GCGTGGATAG
CGGTTTGACT
CACGGGGATT
TCCAAGTCTC
CACCCCATTG
ACGTCAATGG
GAGTTTGTTT
TGGCACCAAA
ATCAACGGGA
CTTTCCAAAA
TGTCGTAACA
ACTCCGCCCC
ATTGACGCAA
ATGGGCGGTA
GGCGTGTACG
GTGGGAGGTC
TATATAAGCA
GAGCTGGTTT
AGTGAACCGT
CAGATCCGCT
AGAGATCCGC
GGCCGCTAAT
ACGACTCACT
ATAGGGAGAG
CCGCCACCAT
GGCCCCAAAG
AAGAAGCGGA
AGGTCGGTAT
CCACGGAGTC
CCAGCAGCCG
CGATGCAGAT
GCAGTTGGAA
GCGAATGCAG
ATACTAGTGT
CGAGGAAGAG
TCATTTGGCC
CGCAACCCAT
CTCGCGTTTA
GAGCAATGTG
GCATCAATGC
AAACGATGTG
AAAAAATTAG
AGGAAGCTGG
ATTCCACACG
GTCGAAGCGG
TCGCATACGC
ACCGAAAAAA
GAGCTGATCA
ACATCAAAGG
CATCAGCGAG
GCGAAAGCCG
ATAAGATTCT
TGCAGAGGCG
GCGAAATTAG
TTCCCATGGG
ATTTACGACG
GAGACTGAGT
TCCATCAACG
TCGTTCCGAG
ATCATTCAAA
TCACGACCGG
AAGCAAGGAG
TTGGATAAAC
TGCTTCAAGG
AGGCATCGAA
ACTGGCTCGA
TCACTGAAAT
GTTTGGAGAG
TTTCGTACTG
GCAAAACCCA
GATCTGCCAC
ACATTGGCCG
TCACTTGCCA
GCTTCCGATC
GATCGTGGTG
GGGGCGAGGG
GAAGGCCATG
TACATTGATA
CTGAGGGGAC
GTTTCGTCCC
GAACGCCTTC
TTGCGGTGGC
AGAGCGTTAC
GGCTTGTCAG
GGTCTGATGT
TCTTGACAAT
GTTGCCTATG
CTCGCGCATT
TAATACCGAC
CACCAGACGC
AGCTTCTGTA
CCAAGCAAGC
GCGATGATGG
TAGAGTCTCG
TTACGCTTTA
TTGATTGTTG
ACTCAGCAAC
AGCGTTGTAC
CGCACAGATT
ATTCTGGGCG
CGGCGAATTG
TCGGCACGCC
AAATGCATTT
AGCCCGCTTT
TTGCGTATGT
TGCTGCGCCT
TGCAGACGAA
TTTGGGGTGG
CTGTGGTGAT
CACCAACCAA
GTTGTAGCAC
AGGTGGACGG
TGCCGCTATG
TTTGCCGCTG
ATCCGAAAAA
ACCCATCGGA
GGGAATATTA
TCGCACATGC
ATCGACAACT
CGCTTATATC
TGCGTAAGGG
CCGTGGAGAA
ACACGCATCT
GCAAAATCTA
TGACTCCCCA
TGCCTGCCGG
AGGCGGAAGC
CATGTTCGCA
ATTAACGCAG
ATGGCGTAGG
GGACGCCAAA
GATTCTGGTG
GTTCTTCTGG
TGGTTCTAGC
GGCAGCGAGA
CTCCCGGGAC
CTCAGAGTCC
GCCACACCCG
AAAGTTCTGG
TGGTTCTTCT
GGTGGTTCTG
ACAAGAAGTA
CAGCATCGGC
CTGGCCATCG
GCACCAACTC
TGTGGGCTGG
GCCGTGATCA
CCGACGAGTA
CAAGGTGCCC
AGCAAGAAAT
TCAAGGTGCT
GGGCAACACC
GACCGGCACA
GCATCAAGAA
GAACCTGATC
GGAGCCCTGC
TGTTCGACAG
CGGCGAAACA
GCCGAGGCCA
CCCGGCTGAA
GAGAACCGCC
AGAAGAAGAT
ACACCAGACG
GAAGAACCGG
ATCTGCTATC
TGCAAGAGAT
CTTCAGCAAC
GAGATGGCCA
AGGTGGACGA
CAGCTTCTTC
CACAGACTGG
AAGAGTCCTT
CCTGGTGGAA
GAGGATAAGA
AGCACGAGCG
GCACCCCATC
TTCGGCAACA
TCGTGGACGA
GGTGGCCTAC
CACGAGAAGT
ACCCCACCAT
CTACCACCTG
AGAAAGAAAC
TGGTGGACAG
CACCGACAAG
GCCGACCTGC
GGCTGATCTA
TCTGGCCCTG
GCCCACATGA
TCAAGTTCCG
GGGCCACTTC
CTGATCGAGG
GCGACCTGAA
CCCCGACAAC
AGCGACGTGG
ACAAGCTGTT
CATCCAGCTG
GTGCAGACCT
ACAACCAGCT
GTTCGAGGAA
AACCCCATCA
ACGCCAGCGG
CGTGGACGCC
AAGGCCATCC
TGTCTGCCAG
ACTGAGCAAG
AGCAGACGGC
TGGAAAATCT
GATCGCCCAG
CTGCCCGGCG
AGAAGAAGAA
TGGCCTGTTC
GGAAACCTGA
TTGCCCTGAG
CCTGGGCCTG
ACCCCCAACT
TCAAGAGCAA
CTTCGACCTG
GCCGAGGATG
CCAAACTGCA
GCTGAGCAAG
GACACCTACG
ACGACGACCT
GGACAACCTG
CTGGCCCAGA
TCGGCGACCA
GTACGCCGAC
CTGTTTCTGG
CCGCCAAGAA
CCTGTCCGAC
GCCATCCTGC
TGAGCGACAT
CCTGAGAGTG
AACACCGAGA
TCACCAAGGC
CCCCCTGAGC
GCCTCTATGA
TCAAGAGATA
CGACGAGCAC
CACCAGGACC
TGACCCTGCT
GAAAGCTCTC
GTGCGGCAGC
AGCTGCCTGA
GAAGTACAAA
GAGATTTTCT
TCGACCAGAG
CAAGAACGGC
TACGCCGGCT
ACATTGACGG
CGGAGCCAGC
CAGGAAGAGT
TCTACAAGTT
CATCAAGCCC
ATCCTGGAAA
AGATGGACGG
CACCGAGGAA
CTGCTCGTGA
AGCTGAACAG
AGAGGACCTG
CTGCGGAAGC
AGCGGACCTT
CGACAACGGC
AGCATCCCCC
ACCAGATCCA
CCTGGGAGAG
CTGCACGCCA
TTCTGCGGCG
GCAGGAAGAT
TTTTACCCAT
TCCTGAAGGA
CAACCGGGAA
AAGATCGAGA
AGATCCTGAC
CTTCCGCATA
CCCTACTACG
TGGGCCCTCT
GGCCAGGGGA
AACAGCAGAT
TCGCCTGGAT
GACCAGAAAG
AGCGAGGAAA
CCATCACCCC
CTGGAACTTC
GAGGAAGTGG
TGGACAAGGG
CGCTTCCGCC
CAGAGCTTCA
TCGAGCGGAT
GACCAACTTC
GATAAGAACC
TGCCCAACGA
GAAGGTGCTG
CCCAAGCACA
GCCTGCTGTA
CGAGTACTTC
ACCGTGTATA
ACGAGCTGAC
CAAAGTGAAA
TACGTGACCG
AGGGAATGAG
AAAGCCCGCC
TTCCTGAGCG
GCGAGCAGAA
AAAGGCCATC
GTGGACCTGC
TGTTCAAGAC
CAACCGGAAA
GTGACCGTGA
AGCAGCTGAA
AGAGGACTAC
TTCAAGAAAA
TCGAGTGCTT
CGACTCCGTG
GAAATCTCCG
GCGTGGAAGA
TCGGTTCAAC
GCCTCCCTGG
GCACATACCA
CGATCTGCTG
AAAATTATCA
AGGACAAGGA
CTTCCTGGAC
AATGAGGAAA
ACGAGGACAT
TCTGGAAGAT
ATCGTGCTGA
CCCTGACACT
GTTTGAGGAC
AGAGAGATGA
TCGAGGAACG
GCTGAAAACC
TATGCCCACC
TGTTCGACGA
CAAAGTGATG
AAGCAGCTGA
AGCGGCGGAG
ATACACCGGC
TGGGGCAGGC
TGAGCCGGAA
GCTGATCAAC
GGCATCCGGG
ACAAGCAGTC
CGGCAAGACA
ATCCTGGATT
TCCTGAAGTC
CGACGGCTTC
GCCAACAGAA
ACTTCATGCA
GCTGATCCAC
GACGACAGCC
TGACCTTTAA
AGAGGACATC
CAGAAAGCCC
AGGTGTCCGG
CCAGGGCGAT
AGCCTGCACG
AGCACATTGC
CAATCTGGCC
GGCAGCCCCG
CCATTAAGAA
GGGCATCCTG
CAGACAGTGA
AGGTGGTGGA
CGAGCTCGTG
AAAGTGATGG
GCCGGCACAA
GCCCGAGAAC
ATCGTGATCG
AAATGGCCAG
AGAGAACCAG
ACCACCCAGA
AGGGACAGAA
GAACAGCCGC
GAGAGAATGA
AGCGGATCGA
AGAGGGCATC
AAAGAGCTGG
GCAGCCAGAT
CCTGAAAGAA
CACCCCGTGG
AAAACACCCA
GCTGCAGAAC
GAGAAGCTGT
ACCTGTACTA
CCTGCAGAAT
GGGCGGGATA
TGTACGTGGA
CCAGGAACTG
GACATCAACC
GGCTGTCCGA
CTACGATGTG
GACCATATCG
TGCCTCAGAG
CTTTCTGAAG
GACGACTCCA
TCGACAACAA
GGTGCTGACC
AGAAGCGACA
AGAACCGGGG
CAAGAGCGAC
AACGTGCCCT
CCGAAGAGGT
CGTGAAGAAG
ATGAAGAACT
ACTGGCGGCA
GCTGCTGAAC
GCCAAGCTGA
TTACCCAGAG
AAAGTTCGAC
AATCTGACCA
AGGCCGAGAG
AGGCGGCCTG
AGCGAACTGG
ATAAGGCCGG
CTTCATCAAG
AGACAGCTGG
TGGAAACCCG
GCAGATCACA
AAGCACGTGG
CACAGATCCT
GGACTCCCGG
ATGAACACTA
AGTACGACGA
GAATGACAAG
CTGATCCGGG
AAGTGAAAGT
GATCACCCTG
AAGTCCAAGC
TGGTGTCCGA
TTTCCGGAAG
GATTTCCAGT
TTTACAAAGT
GCGCGAGATC
AACAACTACC
ACCACGCCCA
CGACGCCTAC
CTGAACGCCG
TCGTGGGAAC
CGCCCTGATC
AAAAAGTACC
CTAAGCTGGA
AAGCGAGTTC
GTGTACGGCG
ACTACAAGGT
GTACGACGTG
CGGAAGATGA
TCGCCAAGAG
CGAGCAGGAA
ATCGGCAAGG
CTACCGCCAA
GTACTTCTTC
TACAGCAACA
TCATGAACTT
TTTCAAGACC
GAGATTACCC
TGGCCAACGG
CGAGATCCGG
AAGCGGCCTC
TGATCGAGAC
AAACGGCGAA
ACCGGGGAGA
TCGTGTGGGA
TAAGGGCCGG
GATTTTGCCA
CCGTGCGGAA
AGTGCTGAGC
ATGCCCCAAG
TGAATATCGT
GAAAAAGACC
GAGGTGCAGA
CAGGCGGCTT
CAGCAAAGAG
TCTATCCTGC
CCAAGAGGAA
CAGCGATAAG
CTGATCGCCA
GAAAGAAGGA
CTGGGACCCT
AAGAAGTACG
GCGGCTTCGA
CAGCCCCACC
GTGGCCTATT
CTGTGCTGGT
GGTGGCCAAA
GTGGAAAAGG
GCAAGTCCAA
GAAACTGAAG
AGTGTGAAAG
AGCTGCTGGG
GATCACCATC
ATGGAAAGAA
GCAGCTTCGA
GAAGAATCCC
ATCGACTTTC
TGGAAGCCAA
GGGCTACAAA
GAAGTGAAAA
AGGACCTGAT
CATCAAGCTG
CCTAAGTACT
CCCTGTTCGA
GCTGGAAAAC
GGCCGGAAGA
GAATGCTGGC
CTCTGCCGGC
GAACTGCAGA
AGGGAAACGA
ACTGGCCCTG
CCCTCCAAAT
ATGTGAACTT
CCTGTACCTG
GCCAGCCACT
ATGAGAAGCT
GAAGGGCTCC
CCCGAGGATA
ATGAGCAGAA
ACAGCTGTTT
GTGGAACAGC
ACAAGCACTA
CCTGGACGAG
ATCATCGAGC
AGATCAGCGA
GTTCTCCAAG
AGAGTGATCC
TGGCCGACGC
TAATCTGGAC
AAAGTGCTGT
CCGCCTACAA
CAAGCACCGG
GATAAGCCCA
TCAGAGAGCA
GGCCGAGAAT
ATCATCCACC
TGTTTACCCT
GACCAATCTG
GGAGCCCCTG
CCGCCTTCAA
GTACTTTGAC
ACCACCATCG
ACCGGAAGAG
GTACACCAGC
ACCAAAGAGG
TGCTGGACGC
CACCCTGATC
CACCAGAGCA
TCACCGGCCT
GTACGAGACA
CGGATCGACC
TGTCTCAGCT
GGGAGGCGAC
AAAAGGCCGG
CGGCCACGAA
AAAGGCCGGC
CAGGCAAAAA
AGAAAAAGTG
ACCGGTCATC
ATCACCATCA
CCATTGAGTT
TAAACCCGCT
GATCAGCCTC
GACTGTGCCT
TCTAGTTGCC
AGCCATCTGT
TGTTTGCCCC
TCCCCCGTGC
CTTCCTTGAC
CCTGGAAGGT
GCCACTCCCA
CTGTCCTTTC
CTAATAAAAT
GAGGAAATTG
CATCGCATTG
TCTGAGTAGG
TGTCATTCTA
TTCTGGGGGG
TGGGGTGGGG
CAGGACAGCA
AGGGGGAGGA
TTGGGAAGAC
AATAGCAGGC
ATGCTGGGGA
TGCGGTGGGC
TCTATGGCTT
CTGAGGCGGA
AAGAACCAGC
TGGGGCTCGA
TACCGTCGAC
CTCTAGCTAG
AGCTTGGCGT
AATCATGGTC
ATAGCTGTTT
CCTGTGTGAA
ATTGTTATCC
GCTCACAATT
CCACACAACA
TACGAGCCGG
AAGCATAAAG
TGTAAAGCCT
AGGGTGCCTA
ATGAGTGAGC
TAACTCACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCTGCATT
AATGAATCGG
CCAACGCGCG
GGGAGAGGCG
GTTTGCGTAT
TGGGCGCTCT
TCCGCTTCCT
CGCTCACTGA
CTCGCTGCGC
TCGGTCGTTC
GGCTGCGGCG
AGCGGTATCA
GCTCACTCAA
AGGCGGTAAT
ACGGTTATCC
ACAGAATCAG
GGGATAACGC
AGGAAAGAAC
ATGTGAGCAA
AAGGCCAGCA
AAAGGCCAGG
AACCGTAAAA
AGGCCGCGTT
GCTGGCGTTT
TTCCATAGGC
TCCGCCCCCC
TGACGAGCAT
CACAAAAATC
GACGCTCAAG
TCAGAGGTGG
CGAAACCCGA
CAGGACTATA
AAGATACCAG
GCGTTTCCCC
CTGGAAGCTC
CCTCGTGCGC
TCTCCTGTTC
CGACCCTGCC
GCTTACCGGA
TACCTGTCCG
CCTTTCTCCC
TTCGGGAAGC
GTGGCGCTTT
CTCATAGCTC
ACGCTGTAGG
TATCTCAGTT
CGGTGTAGGT
CGTTCGCTCC
AAGCTGGGCT
GTGTGCACGA
ACCCCCCGTT
CAGCCCGACC
GCTGCGCCTT
ATCCGGTAAC
TATCGTCTTG
AGTCCAACCC
GGTAAGACAC
GACTTATCGC
CACTGGCAGC
AGCCACTGGT
AACAGGATTA
GCAGAGCGAG
GTATGTAGGC
GGTGCTACAG
AGTTCTTGAA
GTGGTGGCCT
AACTACGGCT
ACACTAGAAG
AACAGTATTT
GGTATCTGCG
CTCTGCTGAA
GCCAGTTACC
TTCGGAAAAA
GAGTTGGTAG
CTCTTGATCC
GGCAAACAAA
CCACCGCTGG
TAGCGGTGGT
TTTTTTGTTT
GCAAGCAGCA
GATTACGCGC
AGAAAAAAAG
GATCTCAAGA
AGATCCTTTG
ATCTTTTCTA
CGGGGTCTGA
CGCTCAGTGG
AACGAAAACT
CACGTTAAGG
GATTTTGGTC
ATGAGATTAT
CAAAAAGGAT
CTTCACCTAG
ATCCTTTTAA
ATTAAAAATG
AAGTTTTAAA
TCAATCTAAA
GTATATATGA
GTAAACTTGG
TCTGACAGTT
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GTGCTGCAAT
GATACCGCGA
GACCCACGCT
CACCGGCTCC
AGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
TGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCACTGCAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAATACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
CTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTTC
CGCGCACATT
TCCCCGAAAA
GTGCCACCTG
ACGTCGACGG
ATCGGGAGAT
CGATCTCCCG
ATCCCCTAGG
GTCGACTCTC
AGTACAATCT
GCTCTGATGC
CGCATAGTTA
AGCCAGTATC
TGCTCCCTGC
TTGTGTGTTG
GAGGTCGCTG
AGTAGTGCGC
GAGCAAAATT
TAAGCTACAA
CAAGGCAAGG
CTTGACCGAC
AATTGCATGA
AGAATCTGCT
TAGGGTTAGG
CGTTTTGCGC
TGCTTCGCGA
TGTACGGGCC
AGATATACGC
GTTGACATTG
ATTATTGACT
AGTTATTAAT
AGTAATCAAT
TACGGGGTCA
TTAGTTCATA
GCCCATATAT
GGAGTTCCGC
GTTACATAAC
TTACGGTAAA
TGGCCCGCCT
GGCTGACCGC
CCAACGACCC
CCGCCCATTG
ACGTCAATAA
TGACGTATGT
TCCCATAGTA
ACGCCAATAG
GGACTTTCCA
TTGACGTCAA
TGGGTGGAGT
ATTTACGGTA
AACTGCCCAC
TTGGCAGTAC
ATCAAGTGTA
TC
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