名称: | pAAV2/rh10 |
---|---|
别称: | Plasmid #112866 |
基因长: | |
质粒宿主: | 哺乳细胞,腺相关病毒 |
质粒用途: | 包装辅助 |
片段类型: | ORF |
片段物种: | 病毒 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | P5 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GCGTCCCATT
CGCCATTCAG
GCTGCGCAAC
TGTTGGGAAG
GGCGATCGGT
GCGGGCCTCT
TCGCTATTAC
GCCAGCTGGC
GAAAGGGGGA
TGTGCTGCAA
GGCGATTAAG
TTGGGTAACG
CCAGGGTTTT
CCCAGTCACG
ACGTTGTAAA
ACGACGGCCA
GTGAGCGCGC
GTAATACGAC
TCACTATAGG
GCGAATTGGG
TACCGGGCCC
CCCCTCGATC
GAGGTCGACG
GTATCGGGGG
AGCTCGCAGG
GTCTCCATTT
TGAAGCGGGA
GGTTTGAACG
CGCAGCCGCC
ATGCCGGGGT
TTTACGAGAT
TGTGATTAAG
GTCCCCAGCG
ACCTTGACGA
GCATCTGCCC
GGCATTTCTG
ACAGCTTTGT
GAACTGGGTG
GCCGAGAAGG
AATGGGAGTT
GCCGCCAGAT
TCTGACATGG
ATCTGAATCT
GATTGAGCAG
GCACCCCTGA
CCGTGGCCGA
GAAGCTGCAG
CGCGACTTTC
TGACGGAATG
GCGCCGTGTG
AGTAAGGCCC
CGGAGGCTCT
TTTCTTTGTG
CAATTTGAGA
AGGGAGAGAG
CTACTTCCAC
ATGCACGTGC
TCGTGGAAAC
CACCGGGGTG
AAATCCATGG
TTTTGGGACG
TTTCCTGAGT
CAGATTCGCG
AAAAACTGAT
TCAGAGAATT
TACCGCGGGA
TCGAGCCGAC
TTTGCCAAAC
TGGTTCGCGG
TCACAAAGAC
CAGAAATGGC
GCCGGAGGCG
GGAACAAGGT
GGTGGATGAG
TGCTACATCC
CCAATTACTT
GCTCCCCAAA
ACCCAGCCTG
AGCTCCAGTG
GGCGTGGACT
AATATGGAAC
AGTATTTAAG
CGCCTGTTTG
AATCTCACGG
AGCGTAAACG
GTTGGTGGCG
CAGCATCTGA
CGCACGTGTC
GCAGACGCAG
GAGCAGAACA
AAGAGAATCA
GAATCCCAAT
TCTGATGCGC
CGGTGATCAG
ATCAAAAACT
TCAGCCAGGT
ACATGGAGCT
GGTCGGGTGG
CTCGTGGACA
AGGGGATTAC
CTCGGAGAAG
CAGTGGATCC
AGGAGGACCA
GGCCTCATAC
ATCTCCTTCA
ATGCGGCCTC
CAACTCGCGG
TCCCAAATCA
AGGCTGCCTT
GGACAATGCG
GGAAAGATTA
TGAGCCTGAC
TAAAACCGCC
CCCGACTACC
TGGTGGGCCA
GCAGCCCGTG
GAGGACATTT
CCAGCAATCG
GATTTATAAA
ATTTTGGAAC
TAAACGGGTA
CGATCCCCAA
TATGCGGCTT
CCGTCTTTCT
GGGATGGGCC
ACGAAAAAGT
TCGGCAAGAG
GAACACCATC
TGGCTGTTTG
GGCCTGCAAC
TACCGGGAAG
ACCAACATCG
CGGAGGCCAT
AGCCCACACT
GTGCCCTTCT
ACGGGTGCGT
AAACTGGACC
AATGAGAACT
TTCCCTTCAA
CGACTGTGTC
GACAAGATGG
TGATCTGGTG
GGAGGAGGGG
AAGATGACCG
CCAAGGTCGT
GGAGTCGGCC
AAAGCCATTC
TCGGAGGAAG
CAAGGTGCGC
GTGGACCAGA
AATGCAAGTC
CTCGGCCCAG
ATAGACCCGA
CTCCCGTGAT
CGTCACCTCC
AACACCAACA
TGTGCGCCGT
GATTGACGGG
AACTCAACGA
CCTTCGAACA
CCAGCAGCCG
TTGCAAGACC
GGATGTTCAA
ATTTGAACTC
ACCCGCCGTC
TGGATCATGA
CTTTGGGAAG
GTCACCAAGC
AGGAAGTCAA
AGACTTTTTC
CGGTGGGCAA
AGGATCACGT
GGTTGAGGTG
GAGCATGAAT
TCTACGTCAA
AAAGGGTGGA
GCCAAGAAAA
GACCCGCCCC
CAGTGACGCA
GATATAAGTG
AGCCCAAACG
GGTGCGCGAG
TCAGTTGCGC
AGCCATCGAC
GTCAGACGCG
GAAGCTTCGA
TCAACTACGC
AGACAGGTAC
CAAAACAAAT
GTTCTCGTCA
CGTGGGCATG
AATCTGATGC
TGTTTCCCTG
CAGACAATGC
GAGAGAATGA
ATCAGAATTC
AAATATCTGC
TTCACTCACG
GACAGAAAGA
CTGTTTAGAG
TGCTTTCCCG
TGTCAGAATC
TCAACCCGTT
TCTGTCGTCA
AAAAGGCGTA
TCAGAAACTG
TGCTACATTC
ATCATATCAT
GGGAAAGGTG
CCAGACGCTT
GCACTGCCTG
CGATCTGGTC
AATGTGGATT
TGGATGACTG
CATCTTTGAA
CAATAAATGA
TTTAAATCAG
GTATGGCTGC
CGATGGTTAT
CTTCCAGATT
GGCTCGAGGA
CAACCTCTCT
GAGGGCATTC
GCGAGTGGTG
GGACTTGAAA
CCTGGAGCCC
CGAAACCCAA
AGCCAACCAG
CAAAAGCAGG
ACGACGGCCG
GGGTCTGGTG
CTTCCTGGCT
ACAAGTACCT
CGGACCCTTC
AACGGACTCG
ACAAGGGGGA
GCCCGTCAAC
GCGGCGGACG
CAGCGGCCCT
CGAGCACGAC
AAGGCCTACG
ACCAGCAGCT
CAAAGCGGGT
GACAATCCGT
ACCTGCGGTA
TAACCACGCC
GACGCCGAGT
TTCAGGAGCG
TCTGCAAGAA
GATACGTCTT
TTGGGGGCAA
CCTCGGGCGA
GCAGTCTTCC
AGGCCAAGAA
GCGGGTTCTC
GAACCTCTCG
GTCTGGTTGA
GGAAGGCGCT
AAGACGGCTC
CTGGAAAGAA
GAGACCGGTA
GAGCCATCAC
CCCAGCGTTC
TCCAGACTCC
TCTACGGGCA
TCGGCAAGAA
AGGCCAGCAG
CCCGCGAAAA
AGAGACTCAA
CTTTGGGCAG
ACTGGCGACT
CAGAGTCAGT
GCCCGACCCT
CAACCAATCG
GAGAACCCCC
CGCAGGCCCC
TCTGGTCTGG
GATCTGGTAC
AATGGCTGCA
GGCGGTGGCG
CTCCAATGGC
AGACAATAAC
GAAGGCGCCG
ACGGAGTGGG
TAGTTCCTCA
GGAAATTGGC
ATTGCGATTC
CACATGGCTG
GGCGACAGAG
TCATCACCAC
CAGCACCCGA
ACCTGGGCCC
TCCCCACCTA
CAACAACCAC
CTCTACAAGC
AAATCTCCAA
CGGGACTTCG
GGAGGAAGCA
CCAACGACAA
CACCTACTTC
GGCTACAGCA
CCCCCTGGGG
GTATTTTGAC
TTTAACAGAT
TCCACTGCCA
CTTCTCACCA
CGTGACTGGC
AGCGACTCAT
CAACAACAAC
TGGGGATTCC
GGCCCAAGAG
ACTCAACTTC
AAGCTCTTCA
ACATCCAGGT
CAAGGAGGTC
ACGCAGAATG
AAGGCACCAA
GACCATCGCC
AATAACCTTA
CCAGCACGAT
TCAGGTCTTT
ACGGACTCGG
AATACCAGCT
CCCGTACGTC
CTCGGCTCTG
CGCACCAGGG
CTGCCTGCCT
CCGTTCCCGG
CGGACGTCTT
CATGATTCCT
CAGTACGGGT
ACCTGACTCT
GAACAATGGC
AGTCAGGCCG
TGGGCCGTTC
CTCCTTCTAC
TGCCTGGAGT
ACTTTCCTTC
TCAAATGCTG
AGAACGGGCA
ACAACTTTGA
GTTCAGCTAC
CAGTTTGAGG
ACGTGCCTTT
TCACAGCAGC
TACGCGCACA
GCCAAAGCCT
GGACCGGCTG
ATGAACCCCC
TCATCGACCA
GTACCTGTAC
TACCTGTCTC
GGACTCAGTC
CACGGGAGGT
ACCGCAGGAA
CTCAGCAGTT
GCTATTTTCT
CAGGCCGGGC
CTAATAACAT
GTCGGCTCAG
GCCAAAAACT
GGCTACCCGG
GCCCTGCTAC
CGGCAGCAAC
GCGTCTCCAC
GACACTGTCG
CAAAATAACA
ACAGCAACTT
TGCCTGGACC
GGTGCCACCA
AGTATCATCT
GAATGGCAGA
GACTCTCTGG
TAAATCCCGG
TGTCGCTATG
GCAACCCACA
AGGACGACGA
AGAGCGATTT
TTTCCGTCCA
GCGGAGTCTT
AATGTTTGGG
AAACAGGGAG
CTGGAAAAGA
CAACGTGGAC
TATAGCAGCG
TTATGCTAAC
CAGTGAGGAA
GAAATTAAAA
CCACCAACCC
AGTGGCCACA
GAACAGTACG
GCGTGGTGGC
CGATAACCTG
CAACAGCAAA
ACGCCGCTCC
TATTGTAGGG
GCCGTCAACA
GTCAAGGAGC
CTTACCTGGC
ATGGTCTGGC
AGAACCGGGA
CGTGTACCTG
CAGGGTCCTA
TCTGGGCCAA
GATTCCTCAC
ACGGACGGAA
ACTTTCATCC
CTCGCCGCTG
ATGGGAGGCT
TTGGACTGAA
ACACCCGCCT
CCTCAGATCC
TGATTAAGAA
TACACCTGTT
CCCGCGGATC
CTCCAACTAC
CTTCAGTCAA
GCTAAGCTGG
CGTCGTTCAT
CACGCAGTAC
AGCACCGGAC
AGGTCAGCGT
GGAAATTGAA
TGGGAGCTGC
AGAAAGAAAA
CAGCAAACGC
TGGAACCCAG
AGATTCAATA
CACTTCCAAC
TACTACAAAT
CTACAAATGT
GGACTTTGCT
GTTAACACAG
ATGGCACTTA
TTCTGAGCCT
CGCCCCATCG
GCACCCGTTA
CCTCACCCGT
AATCTGTAAT
TGCTTGTTAA
TCAATAAACC
GGTTGATTCG
TTTCAGTTGA
ACTTTGGTCT
CTGCGAAGGG
CGAATTCGTT
TAAACCTGCA
GGACTAGAGG
TCCTGTATTA
GAGGTCACGT
GAGTGTTTTG
CGACATTTTG
CGACACCATG
TGGTCACGCT
GGGTATTTAA
GCCCGAGTGA
GCACGCAGGG
TCTCCATTTT
GAAGCGGGAG
GTTTGAACGC
GCAGCCGCCA
AGCCGAATTC
TGCAGATATC
CATCACACTG
GCGGCCGCTC
GACTAGAGCG
GCCGCCACCG
CGGTGGAGCT
CCAGCTTTTG
TTCCCTTTAG
TGAGGGTTAA
TTGCGCGCTT
GGCGTAATCA
TGGTCATAGC
TGTTTCCTGT
GTGAAATTGT
TATCCGCTCA
CAATTCCACA
CAACATACGA
GCCGGAAGCA
TAAAGTGTAA
AGCCTGGGGT
GCCTAATGAG
TGAGCTAACT
CACATTAATT
GCGTTGCGCT
CACTGCCCGC
TTTCCAGTCG
GGAAACCTGT
CGTGCCAGCT
GCATTAATGA
ATCGGCCAAC
GCGCGGGGAG
AGGCGGTTTG
CGTATTGGGC
GCTCTTCCGC
TTCCTCGCTC
ACTGACTCGC
TGCGCTCGGT
CGTTCGGCTG
CGGCGAGCGG
TATCAGCTCA
CTCAAAGGCG
GTAATACGGT
TATCCACAGA
ATCAGGGGAT
AACGCAGGAA
AGAACATGTG
AGCAAAAGGC
CAGCAAAAGG
CCAGGAACCG
TAAAAAGGCC
GCGTTGCTGG
CGTTTTTCCA
TAGGCTCCGC
CCCCCTGACG
AGCATCACAA
AAATCGACGC
TCAAGTCAGA
GGTGGCGAAA
CCCGACAGGA
CTATAAAGAT
ACCAGGCGTT
TCCCCCTGGA
AGCTCCCTCG
TGCGCTCTCC
TGTTCCGACC
CTGCCGCTTA
CCGGATACCT
GTCCGCCTTT
CTCCCTTCGG
GAAGCGTGGC
GCTTTCTCAT
AGCTCACGCT
GTAGGTATCT
CAGTTCGGTG
TAGGTCGTTC
GCTCCAAGCT
GGGCTGTGTG
CACGAACCCC
CCGTTCAGCC
CGACCGCTGC
GCCTTATCCG
GTAACTATCG
TCTTGAGTCC
AACCCGGTAA
GACACGACTT
ATCGCCACTG
GCAGCAGCCA
CTGGTAACAG
GATTAGCAGA
GCGAGGTATG
TAGGCGGTGC
TACAGAGTTC
TTGAAGTGGT
GGCCTAACTA
CGGCTACACT
AGAAGAACAG
TATTTGGTAT
CTGCGCTCTG
CTGAAGCCAG
TTACCTTCGG
AAAAAGAGTT
GGTAGCTCTT
GATCCGGCAA
ACAAACCACC
GCTGGTAGCG
GTGGTTTTTT
TGTTTGCAAG
CAGCAGATTA
CGCGCAGAAA
AAAAGGATCT
CAAGAAGATC
CTTTGATCTT
TTCTACGGGG
TCTGACGCTC
AGTGGAACGA
AAACTCACGT
TAAGGGATTT
TGGTCATGAG
ATTATCAAAA
AGGATCTTCA
CCTAGATCCT
TTTAAATTAA
AAATGAAGTT
TTAAATCAAT
CTAAAGTATA
TATGAGTAAA
CTTGGTCTGA
CAGTTACCAA
TGCTTAATCA
GTGAGGCACC
TATCTCAGCG
ATCTGTCTAT
TTCGTTCATC
CATAGTTGCC
TGACTCCCCG
TCGTGTAGAT
AACTACGATA
CGGGAGGGCT
TACCATCTGG
CCCCAGTGCT
GCAATGATAC
CGCGAGACCC
ACGCTCACCG
GCTCCAGATT
TATCAGCAAT
AAACCAGCCA
GCCGGAAGGG
CCGAGCGCAG
AAGTGGTCCT
GCAACTTTAT
CCGCCTCCAT
CCAGTCTATT
AATTGTTGCC
GGGAAGCTAG
AGTAAGTAGT
TCGCCAGTTA
ATAGTTTGCG
CAACGTTGTT
GCCATTGCTA
CAGGCATCGT
GGTGTCACGC
TCGTCGTTTG
GTATGGCTTC
ATTCAGCTCC
GGTTCCCAAC
GATCAAGGCG
AGTTACATGA
TCCCCCATGT
TGTGCAAAAA
AGCGGTTAGC
TCCTTCGGTC
CTCCGATCGT
TGTCAGAAGT
AAGTTGGCCG
CAGTGTTATC
ACTCATGGTT
ATGGCAGCAC
TGCATAATTC
TCTTACTGTC
ATGCCATCCG
TAAGATGCTT
TTCTGTGACT
GGTGAGTACT
CAACCAAGTC
ATTCTGAGAA
TAGTGTATGC
GGCGACCGAG
TTGCTCTTGC
CCGGCGTCAA
TACGGGATAA
TACCGCGCCA
CATAGCAGAA
CTTTAAAAGT
GCTCATCATT
GGAAAACGTT
CTTCGGGGCG
AAAACTCTCA
AGGATCTTAC
CGCTGTTGAG
ATCCAGTTCG
ATGTAACCCA
CTCGTGCACC
CAACTGATCT
TCAGCATCTT
TTACTTTCAC
CAGCGTTTCT
GGGTGAGCAA
AAACAGGAAG
GCAAAATGCC
GCAAAAAAGG
GAATAAGGGC
GACACGGAAA
TGTTGAATAC
TCATACTCTT
CCTTTTTCAA
TATTATTGAA
GCATTTATCA
GGGTTATTGT
CTCATGAGCG
GATACATATT
TGAATGTATT
TAGAAAAATA
AACAAATAGG
GGTTCCGCGC
ACATTTCCCC
GAAAAGTGCC
ACCTAAATTG
TAAGCGTTAA
TATTTTGTTA
AAATTCGCGT
TAAATTTTTG
TTAAATCAGC
TCATTTTTTA
ACCAATAGGC
CGAAATCGGC
AAAATCCCTT
ATAAATCAAA
AGAATAGACC
GAGATAGGGT
TGAGTGTTGT
TCCAGTTTGG
AACAAGAGTC
CACTATTAAA
GAACGTGGAC
TCCAACGTCA
AAGGGCGAAA
AACCGTCTAT
CAGGGCGATG
GCCCACTACG
TGAACCATCA
CCCTAATCAA
GTTTTTTGGG
GTCGAGGTGC
CGTAAAGCAC
TAAATCGGAA
CCCTAAAGGG
AGCCCCCGAT
TTAGAGCTTG
ACGGGGAAAG
CCGGCGAACG
TGGCGAGAAA
GGAAGGGAAG
AAAGCGAAAG
GAGCGGGCGC
TAGGGCGCTG
GCAAGTGTAG
CGGTCACGCT
GCGCGTAACC
ACCACACCCG
CCGCGCTTAA
TGCGCCGCTA
CAGGGC