名称: | pESC-HIS |
---|---|
别称: | |
基因长: | |
质粒宿主: | 酵母菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 多框空载 |
原核抗性: | Amp |
筛选标记: | HIS4 |
荧光标记: | |
启动子: | GAL1,10 |
复制子: | 2U |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TCGCGCGTTT
CGGTGATGAC
GGTGAAAACC
TCTGACACAT
GCAGCTCCCG
GAGACGGTCA
CAGCTTGTCT
GTAAGCGGAT
GCCGGGAGCA
GACAAGCCCG
TCAGGGCGCG
TCAGCGGGTG
TTGGCGGGTG
TCGGGGCTGG
CTTAACTATG
CGGCATCAGA
GCAGATTGTA
CTGAGAGTGC
ACCATAAATT
CCCGTTTTAA
GAGCTTGGTG
AGCGCTAGGA
GTCACTGCCA
GGTATCGTTT
GAACACGGCA
TTAGTCAGGG
AAGTCATAAC
ACAGTCCTTT
CCCGCAATTT
TCTTTTTCTA
TTACTCTTGG
CCTCCTCTAG
TACACTCTAT
ATTTTTTTAT
GCCTCGGTAA
TGATTTTCAT
TTTTTTTTTT
CCCCTAGCGG
ATGACTCTTT
TTTTTTCTTA
GCGATTGGCA
TTATCACATA
ATGAATTATA
CATTATATAA
AGTAATGTGA
TTTCTTCGAA
GAATATACTA
AAAAATGAGC
AGGCAAGATA
AACGAAGGCA
AAGATGACAG
AGCAGAAAGC
CCTAGTAAAG
CGTATTACAA
ATGAAACCAA
GATTCAGATT
GCGATCTCTT
TAAAGGGTGG
TCCCCTAGCG
ATAGAGCACT
CGATCTTCCC
AGAAAAAGAG
GCAGAAGCAG
TAGCAGAACA
GGCCACACAA
TCGCAAGTGA
TTAACGTCCA
CACAGGTATA
GGGTTTCTGG
ACCATATGAT
ACATGCTCTG
GCCAAGCATT
CCGGCTGGTC
GCTAATCGTT
GAGTGCATTG
GTGACTTACA
CATAGACGAC
CATCACACCA
CTGAAGACTG
CGGGATTGCT
CTCGGTCAAG
CTTTTAAAGA
GGCCCTACTG
GCGCGTGGAG
TAAAAAGGTT
TGGATCAGGA
TTTGCGCCTT
TGGATGAGGC
ACTTTCCAGA
GCGGTGGTAG
ATCTTTCGAA
CAGGCCGTAC
GCAGTTGTCG
AACTTGGTTT
GCAAAGGGAG
AAAGTAGGAG
ATCTCTCTTG
CGAGATGATC
CCGCATTTTC
TTGAAAGCTT
TGCAGAGGCT
AGCAGAATTA
CCCTCCACGT
TGATTGTCTG
CGAGGCAAGA
ATGATCATCA
CCGTAGTGAG
AGTGCGTTCA
AGGCTCTTGC
GGTTGCCATA
AGAGAAGCCA
CCTCGCCCAA
TGGTACCAAC
GATGTTCCCT
CCACCAAAGG
TGTTCTTATG
TAGTGACACC
GATTATTTAA
AGCTGCAGCA
TACGATATAT
ATACATGTGT
ATATATGTAT
ACCTATGAAT
GTCAGTAAGT
ATGTATACGA
ACAGTATGAT
ACTGAAGATG
ACAAGGTAAT
GCATCATTCT
ATACGTGTCA
TTCTGAACGA
GGCGCGCTTT
CCTTTTTTCT
TTTTGCTTTT
TCTTTTTTTT
TCTCTTGAAC
TCGACGGATC
TATGCGGTGT
GAAATACCGC
ACAGATGCGT
AAGGAGAAAA
TACCGCATCA
GGAAATTGTA
AACGTTAATA
TTTTGTTAAA
ATTCGCGTTA
AATTTTTGTT
AAATCAGCTC
ATTTTTTAAC
CAATAGGCCG
AAATCGGCAA
AATCCCTTAT
AAATCAAAAG
AATAGACCGA
GATAGGGTTG
AGTGTTGTTC
CAGTTTGGAA
CAAGAGTCCA
CTATTAAAGA
ACGTGGACTC
CAACGTCAAA
GGGCGAAAAA
CCGTCTATCA
GGGCGATGGC
CCACTACGTG
AACCATCACC
CTAATCAAGT
TTTTTGGGGT
CGAGGTGCCG
TAAAGCACTA
AATCGGAACC
CTAAAGGGAG
CCCCCGATTT
AGAGCTTGAC
GGGGAAAGCC
GGCGAACGTG
GCGAGAAAGG
AAGGGAAGAA
AGCGAAAGGA
GCGGGCGCTA
GGGCGCTGGC
AAGTGTAGCG
GTCACGCTGC
GCGTAACCAC
CACACCCGCC
GCGCTTAATG
CGCCGCTACA
GGGCGCGTCG
CGCCATTCGC
CATTCAGGCT
GCGCAACTGT
TGGGAAGGGC
GATCGGTGCG
GGCCTCTTCG
CTATTACGCC
AGCTGAATTG
GAGCGACCTC
ATGCTATACC
TGAGAAAGCA
ACCTGACCTA
CAGGAAAGAG
TTACTCAAGA
ATAAGAATTT
TCGTTTTAAA
ACCTAAGAGT
CACTTTAAAA
TTTGTATACA
CTTATTTTTT
TTATAACTTA
TTTAATAATA
AAAATCATAA
ATCATAAGAA
ATTCGCTTAT
TTAGAAGTGT
CAACAACGTA
TCTACCAACG
ATTTGACCCT
TTTCCATCTT
TTCGTAAATT
TCTGGCAAGG
TAGACAAGCC
GACAACCTTG
ATTGGAGACT
TGACCAAACC
TCTGGCGAAG
AATTGTTAAT
TAAGAGCTCA
GATCTTATCG
TCGTCATCCT
TGTAATCCAT
CGATACTAGT
GCGGCCGCCC
TTTAGTGAGG
GTTGAATTCG
AATTTTCAAA
AATTCTTACT
TTTTTTTTGG
ATGGACGCAA
AGAAGTTTAA
TAATCATATT
ACATGGCATT
ACCACCATAT
ACATATCCAT
ATACATATCC
ATATCTAATC
TTACTTATAT
GTTGTGGAAA
TGTAAAGAGC
CCCATTATCT
TAGCCTAAAA
AAACCTTCTC
TTTGGAACTT
TCAGTAATAC
GCTTAACTGC
TCATTGCTAT
ATTGAAGTAC
GGATTAGAAG
CCGCCGAGCG
GGTGACAGCC
CTCCGAAGGA
AGACTCTCCT
CCGTGCGTCC
TCGTCTTCAC
CGGTCGCGTT
CCTGAAACGC
AGATGTGCCT
CGCGCCGCAC
TGCTCCGAAC
AATAAAGATT
CTACAATACT
AGCTTTTATG
GTTATGAAGA
GGAAAAATTG
GCAGTAACCT
GGCCCCACAA
ACCTTCAAAT
GAACGAATCA
AATTAACAAC
CATAGGATGA
TAATGCGATT
AGTTTTTTAG
CCTTATTTCT
GGGGTAATTA
ATCAGCGAAG
CGATGATTTT
TGATCTATTA
ACAGATATAT
AAATGCAAAA
ACTGCATAAC
CACTTTAACT
AATACTTTCA
ACATTTTCGG
TTTGTATTAC
TTCTTATTCA
AATGTAATAA
AAGTATCAAC
AAAAAATTGT
TAATATACCT
CTATACTTTA
ACGTCAAGGA
GAAAAAACCC
CGGATCCGTA
ATACGACTCA
CTATAGGGCC
CGGGCGTCGA
CATGGAACAG
AAGTTGATTT
CCGAAGAAGA
CCTCGAGTAA
GCTTGGTACC
GCGGCTAGCT
AAGATCCGCT
CTAACCGAAA
AGGAAGGAGT
TAGACAACCT
GAAGTCTAGG
TCCCTATTTA
TTTTTTTATA
GTTATGTTAG
TATTAAGAAC
GTTATTTATA
TTTCAAATTT
TTCTTTTTTT
TCTGTACAGA
CGCGTGTACG
CATGTAACAT
TATACTGAAA
ACCTTGCTTG
AGAAGGTTTT
GGGACGCTCG
AAGATCCAGC
TGCATTAATG
AATCGGCCAA
CGCGCGGGGA
GAGGCGGTTT
GCGTATTGGG
CGCTCTTCCG
CTTCCTCGCT
CACTGACTCG
CTGCGCTCGG
TCGTTCGGCT
GCGGCGAGCG
GTATCAGCTC
ACTCAAAGGC
GGTAATACGG
TTATCCACAG
AATCAGGGGA
TAACGCAGGA
AAGAACATGT
GAGCAAAAGG
CCAGCAAAAG
GCCAGGAACC
GTAAAAAGGC
CGCGTTGCTG
GCGTTTTTCC
ATAGGCTCCG
CCCCCCTGAC
GAGCATCACA
AAAATCGACG
CTCAAGTCAG
AGGTGGCGAA
ACCCGACAGG
ACTATAAAGA
TACCAGGCGT
TTCCCCCTGG
AAGCTCCCTC
GTGCGCTCTC
CTGTTCCGAC
CCTGCCGCTT
ACCGGATACC
TGTCCGCCTT
TCTCCCTTCG
GGAAGCGTGG
CGCTTTCTCA
TAGCTCACGC
TGTAGGTATC
TCAGTTCGGT
GTAGGTCGTT
CGCTCCAAGC
TGGGCTGTGT
GCACGAACCC
CCCGTTCAGC
CCGACCGCTG
CGCCTTATCC
GGTAACTATC
GTCTTGAGTC
CAACCCGGTA
AGACACGACT
TATCGCCACT
GGCAGCAGCC
ACTGGTAACA
GGATTAGCAG
AGCGAGGTAT
GTAGGCGGTG
CTACAGAGTT
CTTGAAGTGG
TGGCCTAACT
ACGGCTACAC
TAGAAGGACA
GTATTTGGTA
TCTGCGCTCT
GCTGAAGCCA
GTTACCTTCG
GAAAAAGAGT
TGGTAGCTCT
TGATCCGGCA
AACAAACCAC
CGCTGGTAGC
GGTGGTTTTT
TTGTTTGCAA
GCAGCAGATT
ACGCGCAGAA
AAAAAGGATC
TCAAGAAGAT
CCTTTGATCT
TTTCTACGGG
GTCTGACGCT
CAGTGGAACG
AAAACTCACG
TTAAGGGATT
TTGGTCATGA
GATTATCAAA
AAGGATCTTC
ACCTAGATCC
TTTTAAATTA
AAAATGAAGT
TTTAAATCAA
TCTAAAGTAT
ATATGAGTAA
ACTTGGTCTG
ACAGTTACCA
ATGCTTAATC
AGTGAGGCAC
CTATCTCAGC
GATCTGTCTA
TTTCGTTCAT
CCATAGTTGC
CTGACTCCCC
GTCGTGTAGA
TAACTACGAT
ACGGGAGGGC
TTACCATCTG
GCCCCAGTGC
TGCAATGATA
CCGCGAGACC
CACGCTCACC
GGCTCCAGAT
TTATCAGCAA
TAAACCAGCC
AGCCGGAAGG
GCCGAGCGCA
GAAGTGGTCC
TGCAACTTTA
TCCGCCTCCA
TCCAGTCTAT
TAATTGTTGC
CGGGAAGCTA
GAGTAAGTAG
TTCGCCAGTT
AATAGTTTGC
GCAACGTTGT
TGCCATTGCT
ACAGGCATCG
TGGTGTCACG
CTCGTCGTTT
GGTATGGCTT
CATTCAGCTC
CGGTTCCCAA
CGATCAAGGC
GAGTTACATG
ATCCCCCATG
TTGTGCAAAA
AAGCGGTTAG
CTCCTTCGGT
CCTCCGATCG
TTGTCAGAAG
TAAGTTGGCC
GCAGTGTTAT
CACTCATGGT
TATGGCAGCA
CTGCATAATT
CTCTTACTGT
CATGCCATCC
GTAAGATGCT
TTTCTGTGAC
TGGTGAGTAC
TCAACCAAGT
CATTCTGAGA
ATAGTGTATG
CGGCGACCGA
GTTGCTCTTG
CCCGGCGTCA
ATACGGGATA
ATACCGCGCC
ACATAGCAGA
ACTTTAAAAG
TGCTCATCAT
TGGAAAACGT
TCTTCGGGGC
GAAAACTCTC
AAGGATCTTA
CCGCTGTTGA
GATCCAGTTC
GATGTAACCC
ACTCGTGCAC
CCAACTGATC
TTCAGCATCT
TTTACTTTCA
CCAGCGTTTC
TGGGTGAGCA
AAAACAGGAA
GGCAAAATGC
CGCAAAAAAG
GGAATAAGGG
CGACACGGAA
ATGTTGAATA
CTCATACTCT
TCCTTTTTCA
ATATTATTGA
AGCATTTATC
AGGGTTATTG
TCTCATGAGC
GGATACATAT
TTGAATGTAT
TTAGAAAAAT
AAACAAATAG
GGGTTCCGCG
CACATTTCCC
CGAAAAGTGC
CACCTGAACG
AAGCATCTGT
GCTTCATTTT
GTAGAACAAA
AATGCAACGC
GAGAGCGCTA
ATTTTTCAAA
CAAAGAATCT
GAGCTGCATT
TTTACAGAAC
AGAAATGCAA
CGCGAAAGCG
CTATTTTACC
AACGAAGAAT
CTGTGCTTCA
TTTTTGTAAA
ACAAAAATGC
AACGCGAGAG
CGCTAATTTT
TCAAACAAAG
AATCTGAGCT
GCATTTTTAC
AGAACAGAAA
TGCAACGCGA
GAGCGCTATT
TTACCAACAA
AGAATCTATA
CTTCTTTTTT
GTTCTACAAA
AATGCATCCC
GAGAGCGCTA
TTTTTCTAAC
AAAGCATCTT
AGATTACTTT
TTTTCTCCTT
TGTGCGCTCT
ATAATGCAGT
CTCTTGATAA
CTTTTTGCAC
TGTAGGTCCG
TTAAGGTTAG
AAGAAGGCTA
CTTTGGTGTC
TATTTTCTCT
TCCATAAAAA
AAGCCTGACT
CCACTTCCCG
CGTTTACTGA
TTACTAGCGA
AGCTGCGGGT
GCATTTTTTC
AAGATAAAGG
CATCCCCGAT
TATATTCTAT
ACCGATGTGG
ATTGCGCATA
CTTTGTGAAC
AGAAAGTGAT
AGCGTTGATG
ATTCTTCATT
GGTCAGAAAA
TTATGAACGG
TTTCTTCTAT
TTTGTCTCTA
TATACTACGT
ATAGGAAATG
TTTACATTTT
CGTATTGTTT
TCGATTCACT
CTATGAATAG
TTCTTACTAC
AATTTTTTTG
TCTAAAGAGT
AATACTAGAG
ATAAACATAA
AAAATGTAGA
GGTCGAGTTT
AGATGCAAGT
TCAAGGAGCG
AAAGGTGGAT
GGGTAGGTTA
TATAGGGATA
TAGCACAGAG
ATATATAGCA
AAGAGATACT
TTTGAGCAAT
GTTTGTGGAA
GCGGTATTCG
CAATATTTTA
GTAGCTCGTT
ACAGTCCGGT
GCGTTTTTGG
TTTTTTGAAA
GTGCGTCTTC
AGAGCGCTTT
TGGTTTTCAA
AAGCGCTCTG
AAGTTCCTAT
ACTTTCTAGA
GAATAGGAAC
TTCGGAATAG
GAACTTCAAA
GCGTTTCCGA
AAACGAGCGC
TTCCGAAAAT
GCAACGCGAG
CTGCGCACAT
ACAGCTCACT
GTTCACGTCG
CACCTATATC
TGCGTGTTGC
CTGTATATAT
ATATACATGA
GAAGAACGGC
ATAGTGCGTG
TTTATGCTTA
AATGCGTACT
TATATGCGTC
TATTTATGTA
GGATGAAAGG
TAGTCTAGTA
CCTCCTGTGA
TATTATCCCA
TTCCATGCGG
GGTATCGTAT
GCTTCCTTCA
GCACTACCCT
TTAGCTGTTC
TATATGCTGC
CACTCCTCAA
TTGGATTAGT
CTCATCCTTC
AATGCTATCA
TTTCCTTTGA
TATTGGATCA
TCTAAGAAAC
CATTATTATC
ATGACATTAA
CCTATAAAAA
TAGGCGTATC
ACGAGGCCCT
TTCGTC