名称: | pAAV2/2 |
---|---|
别称: | Plasmid#104963 |
基因长: | |
质粒宿主: | 哺乳细胞,腺相关病毒 |
质粒用途: | 包装辅助 |
片段类型: | ORF |
片段物种: | 病毒 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | P5 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACCTACACCG
AACTGAGATA
CCTACAGCGT
GAGCTATGAG
AAAGCGCCAC
GCTTCCCGAA
GGGAGAAAGG
CGGACAGGTA
TCCGGTAAGC
GGCAGGGTCG
GAACAGGAGA
GCGCACGAGG
GAGCTTCCAG
GGGGAAACGC
CTGGTATCTT
TATAGTCCTG
TCGGGTTTCG
CCACCTCTGA
CTTGAGCGTC
GATTTTTGTG
ATGCTCGTCA
GGGGGGCGGA
GCCTATGGAA
AAACGCCAGC
AACGCGGCCT
TTTTACGGTT
CCTGGCCTTT
TGCTGGCCTT
TTGCTCACAT
GTTCTTTCCT
GCGTTATCCC
CTGATTCTGT
GGATAACCGT
ATTACCGCCT
TTGAGTGAGC
TGATACCGCT
CGCCGCAGCC
GAACGACCGA
GCGCAGCGAG
TCAGTGAGCG
AGGAAGCGGA
AGAGCGCCCA
ATACGCAAAC
CGCCTCTCCC
CGCGCGTTGG
CCGATTCATT
AATGCAGCTG
GCACGACAGG
TTTCCCGACT
GGAAAGCGGG
CAGTGAGCGC
AACGCAATTA
ATGTGAGTTA
GCTCACTCAT
TAGGCACCCC
AGGCTTTACA
CTTTATGCTT
CCGGCTCGTA
TGTTGTGTGG
AATTGTGAGC
GGATAACAAT
TTCACACAGG
AAACAGCTAT
GACCATGATT
ACGCCAAGCG
CGCCGATATC
GTTAACGCCC
CGCGCCGGCC
GCTCTAGAAC
TAGTGGATCC
CCCGGAAGAT
CAGAAGTTCC
TATTCCGAAG
TTCCTATTCT
CTAGAAAGTA
TAGGAACTTC
TGATCTATTC
GAGCTCGGTA
CCCCTAGAGT
CCTGTATTAG
AGGTCACGTG
AGTGTTTTGC
GACATTTTGC
GACACCATGT
GGTCACGCTG
GGTATTTAAG
CCCGAGTGAG
CACGCAGGGT
CTCCATTTTG
AAGCGGGAGG
TTTGAACGCG
CAGCCGCCAT
GCCGGGGTTT
TACGAGATTG
TGATTAAGGT
CCCCAGCGAC
CTTGACGAGC
ATCTGCCCGG
CATTTCTGAC
AGCTTTGTGA
ACTGGGTGGC
CGAGAAGGAA
TGGGAGTTGC
CGCCAGATTC
TGACATGGAT
CTGAATCTGA
TTGAGCAGGC
ACCCCTGACC
GTGGCCGAGA
AGCTGCAGCG
CGACTTTCTG
ACGGAATGGC
GCCGTGTGAG
TAAGGCCCCG
GAGGCCCTTT
TCTTTGTGCA
ATTTGAGAAG
GGAGAGAGCT
ACTTCCACAT
GCACGTGCTC
GTGGAAACCA
CCGGGGTGAA
ATCCATGGTT
TTGGGACGTT
TCCTGAGTCA
GATTCGCGAA
AAACTGATTC
AGAGAATTTA
CCGCGGGATC
GAGCCGACTT
TGCCAAACTG
GTTCGCGGTC
ACAAAGACCA
GAAATGGCGC
CGGAGGCGGG
AACAAGGTGG
TGGATGAGTG
CTACATCCCC
AATTACTTGC
TCCCCAAAAC
CCAGCCTGAG
CTCCAGTGGG
CGTGGACTAA
TATGGAACAG
TATTTAAGCG
CCTGTTTGAA
TCTCACGGAG
CGTAAACGGT
TGGTGGCGCA
GCATCTGACG
CACGTGTCGC
AGACGCAGGA
GCAGAACAAA
GAGAATCAGA
ATCCCAATTC
TGATGCGCCG
GTGATCAGAT
CAAAAACTTC
AGCCAGGTAC
ATGGAGCTGG
TCGGGTGGCT
CGTGGACAAG
GGGATTACCT
CGGAGAAGCA
GTGGATCCAG
GAGGACCAGG
CCTCATACAT
CTCCTTCAAT
GCGGCCTCCA
ACTCGCGGTC
CCAAATCAAG
GCTGCCTTGG
ACAATGCGGG
AAAGATTATG
AGCCTGACTA
AAACCGCCCC
CGACTACCTG
GTGGGCCAGC
AGCCCGTGGA
GGACATTTCC
AGCAATCGGA
TTTATAAAAT
TTTGGAACTA
AACGGGTACG
ATCCCCAATA
TGCGGCTTCC
GTCTTTCTGG
GATGGGCCAC
GAAAAAGTTC
GGCAAGAGGA
ACACCATCTG
GCTGTTTGGG
CCTGCAACTA
CCGGGAAGAC
CAACATCGCG
GAGGCCATAG
CCCACACTGT
GCCCTTCTAC
GGGTGCGTAA
ACTGGACCAA
TGAGAACTTT
CCCTTCAACG
ACTGTGTCGA
CAAGATGGTG
ATCTGGTGGG
AGGAGGGGAA
GATGACCGCC
AAGGTCGTGG
AGTCGGCCAA
AGCCATTCTC
GGAGGAAGCA
AGGTGCGCGT
GGACCAGAAA
TGCAAGTCCT
CGGCCCAGAT
AGACCCGACT
CCCGTGATCG
TCACCTCCAA
CACCAACATG
TGCGCCGTGA
TTGACGGGAA
CTCAACGACC
TTCGAACACC
AGCAGCCGTT
GCAAGACCGG
ATGTTCAAAT
TTGAACTCAC
CCGCCGTCTG
GATCATGACT
TTGGGAAGGT
CACCAAGCAG
GAAGTCAAAG
ACTTTTTCCG
GTGGGCAAAG
GATCACGTGG
TTGAGGTGGA
GCATGAATTC
TACGTCAAAA
AGGGTGGAGC
CAAGAAAAGA
CCCGCCCCCA
GTGACGCAGA
TATAAGTGAG
CCCAAACGGG
TGCGCGAGTC
AGTTGCGCAG
CCATCGACGT
CAGACGCGGA
AGCTTCGATC
AACTACGCAG
ACAGGTACCA
AAACAAATGT
TCTCGTCACG
TGGGCATGAA
TCTGATGCTG
TTTCCCTGCA
GACAATGCGA
GAGAATGAAT
CAGAATTCAA
ATATCTGCTT
CACTCACGGA
CAGAAAGACT
GTTTAGAGTG
CTTTCCCGTG
TCAGAATCTC
AACCCGTTTC
TGTCGTCAAA
AAGGCGTATC
AGAAACTGTG
CTACATTCAT
CATATCATGG
GAAAGGTGCC
AGACGCTTGC
ACTGCCTGCG
ATCTGGTCAA
TGTGGATTTG
GATGACTGCA
TCTTTGAACA
ATAAATGATT
TAAATCAGGT
ATGGCTGCCG
ATGGTTATCT
TCCAGATTGG
CTCGAGGACA
CTCTCTCTGA
AGGAATAAGA
CAGTGGTGGA
AGCTCAAACC
TGGCCCACCA
CCACCAAAGC
CCGCAGAGCG
GCATAAGGAC
GACAGCAGGG
GTCTTGTGCT
TCCTGGGTAC
AAGTACCTCG
GACCCTTCAA
CGGACTCGAC
AAGGGAGAGC
CGGTCAACGA
GGCAGACGCC
GCGGCCCTCG
AGCACGACAA
AGCCTACGAC
CGGCAGCTCG
ACAGCGGAGA
CAACCCGTAC
CTCAAGTACA
ACCACGCCGA
CGCGGAGTTT
CAGGAGCGCC
TTAAAGAAGA
TACGTCTTTT
GGGGGCAACC
TCGGACGAGC
AGTCTTCCAG
GCGAAAAAGA
GGGTTCTTGA
ACCTCTGGGC
CTGGTTGAGG
AACCTGTTAA
GACGGCTCCG
GGAAAAAAGA
GGCCGGTAGA
GCACTCTCCT
GTGGAGCCAG
ACTCCTCCTC
GGGAACCGGA
AAGGCGGGCC
AGCAGCCTGC
AAGAAAAAGA
TTGAATTTTG
GTCAGACTGG
AGACGCAGAC
TCAGTACCTG
ACCCCCAGCC
TCTCGGACAG
CCACCAGCAG
CCCCCTCTGG
TCTGGGAACT
AATACGATGG
CTACAGGCAG
TGGCGCACCA
ATGGCAGACA
ATAACGAGGG
CGCCGACGGA
GTGGGTAATT
CCTCGGGAAA
TTGGCATTGC
GATTCCACAT
GGATGGGCGA
CAGAGTCATC
ACCACCAGCA
CCCGAACCTG
GGCCCTGCCC
ACCTACAACA
ACCACCTCTA
CAAACAAATT
TCCAGCCAAT
CAGGAGCCTC
GAACGACAAT
CACTACTTTG
GCTACAGCAC
CCCTTGGGGG
TATTTTGACT
TCAACAGATT
CCACTGCCAC
TTTTCACCAC
GTGACTGGCA
AAGACTCATC
AACAACAACT
GGGGATTCCG
ACCCAAGAGA
CTCAACTTCA
AGCTCTTTAA
CATTCAAGTC
AAAGAGGTCA
CGCAGAATGA
CGGTACGACG
ACGATTGCCA
ATAACCTTAC
CAGCACGGTT
CAGGTGTTTA
CTGACTCGGA
GTACCAGCTC
CCGTACGTCC
TCGGCTCGGC
GCATCAAGGA
TGCCTCCCGC
CGTTCCCAGC
AGACGTCTTC
ATGGTGCCAC
AGTATGGATA
CCTCACCCTG
AACAACGGGA
GTCAGGCAGT
AGGACGCTCT
TCATTTTACT
GCCTGGAGTA
CTTTCCTTCT
CAGATGCTGC
GTACCGGAAA
CAACTTTACC
TTCAGCTACA
CTTTTGAGGA
CGTTCCTTTC
CACAGCAGCT
ACGCTCACAG
CCAGAGTCTG
GACCGTCTCA
TGAATCCTCT
CATCGACCAG
TACCTGTATT
ACTTGAGCAG
AACAAACACT
CCAAGTGGAA
CCACCACGCA
GTCAAGGCTT
CAGTTTTCTC
AGGCCGGAGC
GAGTGACATT
CGGGACCAGT
CTAGGAACTG
GCTTCCTGGA
CCCTGTTACC
GCCAGCAGCG
AGTATCAAAG
ACATCTGCGG
ATAACAACAA
CAGTGAATAC
TCGTGGACTG
GAGCTACCAA
GTACCACCTC
AATGGCAGAG
ACTCTCTGGT
GAATCCGGGC
CCGGCCATGG
CAAGCCACAA
GGACGATGAA
GAAAAGTTTT
TTCCTCAGAG
CGGGGTTCTC
ATCTTTGGGA
AGCAAGGCTC
AGAGAAAACA
AATGTGGACA
TTGAAAAGGT
CATGATTACA
GACGAAGAGG
AAATCAGGAC
AACCAATCCC
GTGGCTACGG
AGCAGTATGG
TTCTGTATCT
ACCAACCTCC
AGAGAGGCAA
CAGACAAGCA
GCTACCGCAG
ATGTCAACAC
ACAAGGCGTT
CTTCCAGGCA
TGGTCTGGCA
GGACAGAGAT
GTGTACCTTC
AGGGGCCCAT
CTGGGCAAAG
ATTCCACACA
CGGACGGACA
TTTTCACCCC
TCTCCCCTCA
TGGGTGGATT
CGGACTTAAA
CACCCTCCTC
CACAGATTCT
CATCAAGAAC
ACCCCGGTAC
CTGCGAATCC
TTCGACCACC
TTCAGTGCGG
CAAAGTTTGC
TTCCTTCATC
ACACAGTACT
CCACGGGACA
GGTCAGCGTG
GAGATCGAGT
GGGAGCTGCA
GAAGGAAAAC
AGCAAACGCT
GGAATCCCGA
AATTCAGTAC
ACTTCCAACT
ACAACAAGTC
TGTTAATGTG
GACTTTACTG
TGGACACTAA
TGGCGTGTAT
TCAGAGCCTC
GCCCCATTGG
CACCAGATAC
CTGACTCGTA
ATCTGTAATT
GCTTGTTAAT
CAATAAACCG
TTTAATTCGT
TTCAGTTGAA
CTTTGGTCTC
TGCGTATTTC
TTTCTTATCT
AGTTTCCATG
GCTACGTAGA
TAAGTAGCAT
GGCGGGTTAA
TCATTAACTA
CAGCCCGGGC
GTTTAAACAG
CGGGCGGAGG
GGTGGAGTCG
TGACGTGAAT
TACGTCATAG
GGTTAGGGAG
AGTCCTGTAT
TAGAGGTCAC
GTGAGTGTTT
TGCGACATTT
TGCGACACCA
TGTGGTCACG
CTGGGTATTT
AAGCCCGAGT
GAGCACGCAG
GGTCTCCATT
TTGAGCGGGA
GGTTTGAACG
AGCGCTGGCG
CGCTCACTGG
CCGTCGTTTT
ACAACGTCGT
GACTGGGAAA
ACCCTGGCGT
TACCCAACTT
AATCGCCTTG
CAGCACATCC
CCCTTTCGCC
AGCTGGCGTA
ATAGCGAAGA
GGCCCGCACC
GATCGCCCTT
CCCAACAGTT
GCGCAGCCTG
AATGGCGAAT
GGAAATTGTA
AGCGTTAATA
TTTTGTTAAA
ATTCGCGTTA
AATTTTTGTT
AAATCAGCTC
ATTTTTTTAA
CCAATAGGCC
GAAATCGGCA
AAATCCCTTA
TAAATCAAAA
GAATAGACCG
AGATAGGGTT
GAGTGTTGTT
CCAGTTTGGA
ACAAGAGTCC
ACTATTAAGA
ACGTGGACTC
CAACGTCAAA
GGGCGAAAAA
CCGTCTATCA
GGGCGATGGC
CCACTACGTG
AACCATCACC
CTAATCAAGT
TTTTTGGGGT
CGAGGTGCCG
TAAAGCACTA
AATCGGAACC
CTAAAGGGAG
CCCCCGATTT
AGAGCTTGAC
GGGGAAAGCC
GGCGAACGTG
GCGAGAAAGG
AAGGGAAGAA
AGCGAAAGGA
GCGGGCGCTA
GGGCGCTGGC
AAGTGTAGCG
GTCACGCTGC
GCGTAACCAC
CACACCCGCC
GCGCTTAATG
CGCCGCTACA
GGGCGCGTCA
GGTGGCACTT
TTCGGGGAAA
TGTGCGCGGA
ACCCCTATTT
GTTTATTTTT
CTAAATACAT
TCAAATATGT
ATCCGCTCAT
GAGACAATAA
CCCTGATAAA
TGCTTCAATA
ATATTGAAAA
AGGAAGAGTA
TGAGTATTCA
ACATTTCCGT
GTCGCCCTTA
TTCCCTTTTT
TGCGGCATTT
TGCCTTCCTG
TTTTTGCTCA
CCCAGAAACG
CTGGTGAAAG
TAAAAGATGC
TGAAGATCAG
TTGGGTGCAC
GAGTGGGTTA
CATCGAACTG
GATCTCAACA
GCGGTAAGAT
CCTTGAGAGT
TTTCGCCCCG
AAGAACGTTT
TCCAATGATG
AGCACTTTTA
AAGTTCTGCT
ATGTGGCGCG
GTATTATCCC
GTATTGACGC
CGGGCAAGAG
CAACTCGGTC
GCCGCATACA
CTATTCTCAG
AATGACTTGG
TTGAGTACTC
ACCAGTCACA
GAAAAGCATC
TTACGGATGG
CATGACAGTA
AGAGAATTAT
GCAGTGCTGC
CATAACCATG
AGTGATAACA
CTGCGGCCAA
CTTACTTCTG
ACAACGATCG
GAGGACCGAA
GGAGCTAACC
GCTTTTTTGC
ACAACATGGG
GGATCATGTA
ACTCGCCTTG
ATCGTTGGGA
ACCGGAGCTG
AATGAAGCCA
TACCAAACGA
CGAGCGTGAC
ACCACGATGC
CTGTAGCAAT
GGCAACAACG
TTGCGCAAAC
TATTAACTGG
CGAACTACTT
ACTCTAGCTT
CCCGGCAACA
ATTAATAGAC
TGGATGGAGG
CGGATAAAGT
TGCAGGACCA
CTTCTGCGCT
CGGCCCTTCC
GGCTGGCTGG
TTTATTGCTG
ATAAATCTGG
AGCCGGTGAG
CGTGGGTCTC
GCGGTATCAT
TGCAGCACTG
GGGCCAGATG
GTAAGCCCTC
CCGTATCGTA
GTTATCTACA
CGACGGGGAG
TCAGGCAACT
ATGGATGAAC
GAAATAGACA
GATCGCTGAG
ATAGGTGCCT
CACTGATTAA
GCATTGGTAA
CTGTCAGACC
AAGTTTACTC
ATATATACTT
TAGATTGATT
TAAAACTTCA
TTTTTAATTT
AAAAGGATCT
AGGTGAAGAT
CCTTTTTGAT
AATCTCATGA
CCAAAATCCC
TTAACGTGAG
TTTTCGTTCC
ACTGAGCGTC
AGACCCCGTA
GAAAAGATCA
AAGGATCTTC
TTGAGATCCT
TTTTTTCTGC
GCGTAATCTG
CTGCTTGCAA
ACAAAAAAAC
CACCGCTACC
AGCGGTGGTT
TGTTTGCCGG
ATCAAGAGCT
ACCAACTCTT
TTTCCGAAGG
TAACTGGCTT
CAGCAGAGCG
CAGATACCAA
ATACTGTTCT
TCTAGTGTAG
CCGTAGTTAG
GCCACCACTT
CAAGAACTCT
GTAGCACCGC
CTACATACCT
CGCTCTGCTA
ATCCTGTTAC
CAGTGGCTGC
TGCCAGTGGC
GATAAGTCGT
GTCTTACCGG
GTTGGACTCA
AGACGATAGT
TACCGGATAA
GGCGCAGCGG
TCGGGCTGAA
CGGGGGGTTC
GTGCACACAG
CCCAGCTTGG
AGCGAACG