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pX854-U6-gRNA-CBh-SpCas9

产品货号:P1138

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pX854-U6-gRNA-CBh-SpCas9
别称: Plasmid#62886
基因长:
质粒宿主: 哺乳细胞
质粒用途: 基因编辑
片段类型: CRISPR
片段物种:
原核抗性: Amp
筛选标记:
荧光标记:
启动子:
复制子:
拷贝数:
感受态: Stbl3
温度: 37度
背景载体:
正向引物:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

GAGGGCCTAT

TTCCCATGAT

TCCTTCATAT

TTGCATATAC

GATACAAGGC

TGTTAGAGAG

61

ATAATTGGAA

TTAATTTGAC

TGTAAACACA

AAGATATTAG

TACAAAATAC

GTGACGTAGA

121

AAGTAATAAT

TTCTTGGGTA

GTTTGCAGTT

TTAAAATTAT

GTTTTAAAAT

GGACTATCAT

181

ATGCTTACCG

TAACTTGAAA

GTATTTCGAT

TTCTTGGCTT

TATATATCTT

GTGGAAAGGA

241

CGAAACACCG

GGTCTTCGAG

AAGACCTGTT

TTAGAGCTAG

AAATAGCAAG

TTAAAATAAG

301

GCTAGTCCGT

TATCAACTTG

AAAAAGTGGC

ACCGAGTCGG

TGCTTTTTTG

TTTTAGAGCT

361

AGAAATAGCA

AGTTAAAATA

AGGCTAGTCC

GTTTTTAGCG

CGTGCGCCAA

TTCTGCAGAC

421

AAATGGCTCT

AGAGGTACCC

GTTACATAAC

TTACGGTAAA

TGGCCCGCCT

GGCTGACCGC

481

CCAACGACCC

CCGCCCATTG

ACGTCAATAG

TAACGCCAAT

AGGGACTTTC

CATTGACGTC

541

AATGGGTGGA

GTATTTACGG

TAAACTGCCC

ACTTGGCAGT

ACATCAAGTG

TATCATATGC

601

CAAGTACGCC

CCCTATTGAC

GTCAATGACG

GTAAATGGCC

CGCCTGGCAT

TGTGCCCAGT

661

ACATGACCTT

ATGGGACTTT

CCTACTTGGC

AGTACATCTA

CGTATTAGTC

ATCGCTATTA

721

CCATGGTCGA

GGTGAGCCCC

ACGTTCTGCT

TCACTCTCCC

CATCTCCCCC

CCCTCCCCAC

781

CCCCAATTTT

GTATTTATTT

ATTTTTTAAT

TATTTTGTGC

AGCGATGGGG

GCGGGGGGGG

841

GGGGGGGGCG

CGCGCCAGGC

GGGGCGGGGC

GGGGCGAGGG

GCGGGGCGGG

GCGAGGCGGA

901

GAGGTGCGGC

GGCAGCCAAT

CAGAGCGGCG

CGCTCCGAAA

GTTTCCTTTT

ATGGCGAGGC

961

GGCGGCGGCG

GCGGCCCTAT

AAAAAGCGAA

GCGCGCGGCG

GGCGGGAGTC

GCTGCGACGC

1021

TGCCTTCGCC

CCGTGCCCCG

CTCCGCCGCC

GCCTCGCGCC

GCCCGCCCCG

GCTCTGACTG

1081

ACCGCGTTAC

TCCCACAGGT

GAGCGGGCGG

GACGGCCCTT

CTCCTCCGGG

CTGTAATTAG

1141

CTGAGCAAGA

GGTAAGGGTT

TAAGGGATGG

TTGGTTGGTG

GGGTATTAAT

GTTTAATTAC

1201

CTGGAGCACC

TGCCTGAAAT

CACTTTTTTT

CAGGTTGGAC

CGGTGCCACC

ATGGCCCCAA

1261

AGAAGAAGCG

GAAGGTCGGT

ATCCACGGAG

TCCCAGCAGC

CGGCGTGCAG

GTGGAGACCA

1321

TCAGCCCCGG

CGACGGCAGA

ACCTTCCCCA

AGAGAGGCCA

GACCTGCGTG

GTGCACTACA

1381

CCGGCATGCT

GGAGGACGGC

AAGAAGTTCG

ACAGCAGCAG

AGACAGAAAC

AAGCCCTTCA

1441

AGTTCATGCT

GGGCAAGCAG

GAGGTGATCA

GAGGCTGGGA

GGAGGGCGTG

GCCCAGATGA

1501

GCGTGGGCCA

GAGAGCCAAG

CTGACCATCA

GCCCCGACTA

CGCCTACGGC

GCCACCGGCC

1561

ACCCCGGCAT

CATCCCTCCT

CACGCCACCC

TGGTGTTCGA

CGTGGAGCTG

CTGAAGCTGG

1621

AGGGTGGCGG

TGGCTCGGGT

GGCGGTGGCT

CGGGTGGCGG

TGGCTCGTGC

TTCGACTCCG

1681

TGGAAATCTC

CGGCGTGGAA

GATCGGTTCA

ACGCCTCCCT

GGGCACATAC

CACGATCTGC

1741

TGAAAATTAT

CAAGGACAAG

GACTTCCTGG

ACAATGAGGA

AAACGAGGAC

ATTCTGGAAG

1801

ATATCGTGCT

GACCCTGACA

CTGTTTGAGG

ACAGAGAGAT

GATCGAGGAA

CGGCTGAAAA

1861

CCTATGCCCA

CCTGTTCGAC

GACAAAGTGA

TGAAGCAGCT

GAAGCGGCGG

AGATACACCG

1921

GCTGGGGCAG

GCTGAGCCGG

AAGCTGATCA

ACGGCATCCG

GGACAAGCAG

TCCGGCAAGA

1981

CAATCCTGGA

TTTCCTGAAG

TCCGACGGCT

TCGCCAACAG

AAACTTCATG

CAGCTGATCC

2041

ACGACGACAG

CCTGACCTTT

AAAGAGGACA

TCCAGAAAGC

CCAGGTGTCC

GGCCAGGGCG

2101

ATAGCCTGCA

CGAGCACATT

GCCAATCTGG

CCGGCAGCCC

CGCCATTAAG

AAGGGCATCC

2161

TGCAGACAGT

GAAGGTGGTG

GACGAGCTCG

TGAAAGTGAT

GGGCCGGCAC

AAGCCCGAGA

2221

ACATCGTGAT

CGAAATGGCC

AGAGAGAACC

AGACCACCCA

GAAGGGACAG

AAGAACAGCC

2281

GCGAGAGAAT

GAAGCGGATC

GAAGAGGGCA

TCAAAGAGCT

GGGCAGCCAG

ATCCTGAAAG

2341

AACACCCCGT

GGAAAACACC

CAGCTGCAGA

ACGAGAAGCT

GTACCTGTAC

TACCTGCAGA

2401

ATGGGCGGGA

TATGTACGTG

GACCAGGAAC

TGGACATCAA

CCGGCTGTCC

GACTACGATG

2461

TGGACCATAT

CGTGCCTCAG

AGCTTTCTGA

AGGACGACTC

CATCGACAAC

AAGGTGCTGA

2521

CCAGAAGCGA

CAAGAACCGG

GGCAAGAGCG

ACAACGTGCC

CTCCGAAGAG

GTCGTGAAGA

2581

AGATGAAGAA

CTACTGGCGG

CAGCTGCTGA

ACGCCAAGCT

GATTACCCAG

AGAAAGTTCG

2641

ACAATCTGAC

CAAGGCCGAG

AGAGGCGGCC

TGAGCGAACT

GGATAAGGCC

GGCTTCATCA

2701

AGAGACAGCT

GGTGGAAACC

CGGCAGATCA

CAAAGCACGT

GGCACAGATC

CTGGACTCCC

2761

GGATGAACAC

TAAGTACGAC

GAGAATGACA

AGCTGATCCG

GGAAGTGAAA

GTGATCACCC

2821

TGAAGTCCAA

GCTGGTGTCC

GATTTCCGGA

AGGATTTCCA

GTTTTACAAA

GTGCGCGAGA

2881

TCAACAACTA

CCACCACGCC

CACGACGCCT

ACCTGAACGC

CGTCGTGGGA

ACCGCCCTGA

2941

TCAAAAAGTA

CCCTAAGCTG

GAAAGCGAGT

TCGTGTACGG

CGACTACAAG

GTGTACGACG

3001

TGCGGAAGAT

GATCGCCAAG

AGCGAGCAGG

AAATCGGCAA

GGCTACCGCC

AAGTACTTCT

3061

TCTACAGCAA

CATCATGAAC

TTTTTCAAGA

CCGAGATTAC

CCTGGCCAAC

GGCGAGATCC

3121

GGAAGCGGCC

TCTGATCGAG

ACAAACGGCG

AAACCGGGGA

GATCGTGTGG

GATAAGGGCC

3181

GGGATTTTGC

CACCGTGCGG

AAAGTGCTGA

GCATGCCCCA

AGTGAATATC

GTGAAAAAGA

3241

CCGAGGTGCA

GACAGGCGGC

TTCAGCAAAG

AGTCTATCCT

GCCCAAGAGG

AACAGCGATA

3301

AGCTGATCGC

CAGAAAGAAG

GACTGGGACC

CTAAGAAGTA

CGGCGGCTTC

GACAGCCCCA

3361

CCGTGGCCTA

TTCTGTGCTG

GTGGTGGCCA

AAGTGGAAAA

GGGCAAGTCC

AAGAAACTGA

3421

AGAGTGTGAA

AGAGCTGCTG

GGGATCACCA

TCATGGAAAG

AAGCAGCTTC

GAGAAGAATC

3481

CCATCGACTT

TCTGGAAGCC

AAGGGCTACA

AAGAAGTGAA

AAAGGACCTG

ATCATCAAGC

3541

TGCCTAAGTA

CTCCCTGTTC

GAGCTGGAAA

ACGGCCGGAA

GAGAATGCTG

GCCTCTGCCG

3601

GCGAACTGCA

GAAGGGAAAC

GAACTGGCCC

TGCCCTCCAA

ATATGTGAAC

TTCCTGTACC

3661

TGGCCAGCCA

CTATGAGAAG

CTGAAGGGCT

CCCCCGAGGA

TAATGAGCAG

AAACAGCTGT

3721

TTGTGGAACA

GCACAAGCAC

TACCTGGACG

AGATCATCGA

GCAGATCAGC

GAGTTCTCCA

3781

AGAGAGTGAT

CCTGGCCGAC

GCTAATCTGG

ACAAAGTGCT

GTCCGCCTAC

AACAAGCACC

3841

GGGATAAGCC

CATCAGAGAG

CAGGCCGAGA

ATATCATCCA

CCTGTTTACC

CTGACCAATC

3901

TGGGAGCCCC

TGCCGCCTTC

AAGTACTTTG

ACACCACCAT

CGACCGGAAG

AGGTACACCA

3961

GCACCAAAGA

GGTGCTGGAC

GCCACCCTGA

TCCACCAGAG

CATCACCGGC

CTGTACGAGA

4021

CACGGATCGA

CCTGTCTCAG

CTGGGAGGCG

ACAAAAGGCC

GGCGGCCACG

AAAAAGGCCG

4081

GCCAGGCAAA

AAAGAAAAAG

TAAGAATTCC

TAGAGCTCGC

TGATCAGCCT

CGACTGTGCC

4141

TTCTAGTTGC

CAGCCATCTG

TTGTTTGCCC

CTCCCCCGTG

CCTTCCTTGA

CCCTGGAAGG

4201

TGCCACTCCC

ACTGTCCTTT

CCTAATAAAA

TGAGGAAATT

GCATCGCATT

GTCTGAGTAG

4261

GTGTCATTCT

ATTCTGGGGG

GTGGGGTGGG

GCAGGACAGC

AAGGGGGAGG

ATTGGGAAGA

4321

GAATAGCAGG

CATGCTGGGG

AGCGGCCGCA

GGAACCCCTA

GTGATGGAGT

TGGCCACTCC

4381

CTCTCTGCGC

GCTCGCTCGC

TCACTGAGGC

CGGGCGACCA

AAGGTCGCCC

GACGCCCGGG

4441

CTTTGCCCGG

GCGGCCTCAG

TGAGCGAGCG

AGCGCGCAGC

TGCCTGCAGG

GGCGCCTGAT

4501

GCGGTATTTT

CTCCTTACGC

ATCTGTGCGG

TATTTCACAC

CGCATACGTC

AAAGCAACCA

4561

TAGTACGCGC

CCTGTAGCGG

CGCATTAAGC

GCGGCGGGTG

TGGTGGTTAC

GCGCAGCGTG

4621

ACCGCTACAC

TTGCCAGCGC

CCTAGCGCCC

GCTCCTTTCG

CTTTCTTCCC

TTCCTTTCTC

4681

GCCACGTTCG

CCGGCTTTCC

CCGTCAAGCT

CTAAATCGGG

GGCTCCCTTT

AGGGTTCCGA

4741

TTTAGTGCTT

TACGGCACCT

CGACCCCAAA

AAACTTGATT

TGGGTGATGG

TTCACGTAGT

4801

GGGCCATCGC

CCTGATAGAC

GGTTTTTCGC

CCTTTGACGT

TGGAGTCCAC

GTTCTTTAAT

4861

AGTGGACTCT

TGTTCCAAAC

TGGAACAACA

CTCAACCCTA

TCTCGGGCTA

TTCTTTTGAT

4921

TTATAAGGGA

TTTTGCCGAT

TTCGGCCTAT

TGGTTAAAAA

ATGAGCTGAT

TTAACAAAAA

4981

TTTAACGCGA

ATTTTAACAA

AATATTAACG

TTTACAATTT

TATGGTGCAC

TCTCAGTACA

5041

ATCTGCTCTG

ATGCCGCATA

GTTAAGCCAG

CCCCGACACC

CGCCAACACC

CGCTGACGCG

5101

CCCTGACGGG

CTTGTCTGCT

CCCGGCATCC

GCTTACAGAC

AAGCTGTGAC

CGTCTCCGGG

5161

AGCTGCATGT

GTCAGAGGTT

TTCACCGTCA

TCACCGAAAC

GCGCGAGACG

AAAGGGCCTC

5221

GTGATACGCC

TATTTTTATA

GGTTAATGTC

ATGATAATAA

TGGTTTCTTA

GACGTCAGGT

5281

GGCACTTTTC

GGGGAAATGT

GCGCGGAACC

CCTATTTGTT

TATTTTTCTA

AATACATTCA

5341

AATATGTATC

CGCTCATGAG

ACAATAACCC

TGATAAATGC

TTCAATAATA

TTGAAAAAGG

5401

AAGAGTATGA

GTATTCAACA

TTTCCGTGTC

GCCCTTATTC

CCTTTTTTGC

GGCATTTTGC

5461

CTTCCTGTTT

TTGCTCACCC

AGAAACGCTG

GTGAAAGTAA

AAGATGCTGA

AGATCAGTTG

5521

GGTGCACGAG

TGGGTTACAT

CGAACTGGAT

CTCAACAGCG

GTAAGATCCT

TGAGAGTTTT

5581

CGCCCCGAAG

AACGTTTTCC

AATGATGAGC

ACTTTTAAAG

TTCTGCTATG

TGGCGCGGTA

5641

TTATCCCGTA

TTGACGCCGG

GCAAGAGCAA

CTCGGTCGCC

GCATACACTA

TTCTCAGAAT

5701

GACTTGGTTG

AGTACTCACC

AGTCACAGAA

AAGCATCTTA

CGGATGGCAT

GACAGTAAGA

5761

GAATTATGCA

GTGCTGCCAT

AACCATGAGT

GATAACACTG

CGGCCAACTT

ACTTCTGACA

5821

ACGATCGGAG

GACCGAAGGA

GCTAACCGCT

TTTTTGCACA

ACATGGGGGA

TCATGTAACT

5881

CGCCTTGATC

GTTGGGAACC

GGAGCTGAAT

GAAGCCATAC

CAAACGACGA

GCGTGACACC

5941

ACGATGCCTG

TAGCAATGGC

AACAACGTTG

CGCAAACTAT

TAACTGGCGA

ACTACTTACT

6001

CTAGCTTCCC

GGCAACAATT

AATAGACTGG

ATGGAGGCGG

ATAAAGTTGC

AGGACCACTT

6061

CTGCGCTCGG

CCCTTCCGGC

TGGCTGGTTT

ATTGCTGATA

AATCTGGAGC

CGGTGAGCGT

6121

GGAAGCCGCG

GTATCATTGC

AGCACTGGGG

CCAGATGGTA

AGCCCTCCCG

TATCGTAGTT

6181

ATCTACACGA

CGGGGAGTCA

GGCAACTATG

GATGAACGAA

ATAGACAGAT

CGCTGAGATA

6241

GGTGCCTCAC

TGATTAAGCA

TTGGTAACTG

TCAGACCAAG

TTTACTCATA

TATACTTTAG

6301

ATTGATTTAA

AACTTCATTT

TTAATTTAAA

AGGATCTAGG

TGAAGATCCT

TTTTGATAAT

6361

CTCATGACCA

AAATCCCTTA

ACGTGAGTTT

TCGTTCCACT

GAGCGTCAGA

CCCCGTAGAA

6421

AAGATCAAAG

GATCTTCTTG

AGATCCTTTT

TTTCTGCGCG

TAATCTGCTG

CTTGCAAACA

6481

AAAAAACCAC

CGCTACCAGC

GGTGGTTTGT

TTGCCGGATC

AAGAGCTACC

AACTCTTTTT

6541

CCGAAGGTAA

CTGGCTTCAG

CAGAGCGCAG

ATACCAAATA

CTGTCCTTCT

AGTGTAGCCG

6601

TAGTTAGGCC

ACCACTTCAA

GAACTCTGTA

GCACCGCCTA

CATACCTCGC

TCTGCTAATC

6661

CTGTTACCAG

TGGCTGCTGC

CAGTGGCGAT

AAGTCGTGTC

TTACCGGGTT

GGACTCAAGA

6721

CGATAGTTAC

CGGATAAGGC

GCAGCGGTCG

GGCTGAACGG

GGGGTTCGTG

CACACAGCCC

6781

AGCTTGGAGC

GAACGACCTA

CACCGAACTG

AGATACCTAC

AGCGTGAGCT

ATGAGAAAGC

6841

GCCACGCTTC

CCGAAGGGAG

AAAGGCGGAC

AGGTATCCGG

TAAGCGGCAG

GGTCGGAACA

6901

GGAGAGCGCA

CGAGGGAGCT

TCCAGGGGGA

AACGCCTGGT

ATCTTTATAG

TCCTGTCGGG

6961

TTTCGCCACC

TCTGACTTGA

GCGTCGATTT

TTGTGATGCT

CGTCAGGGGG

GCGGAGCCTA

7021

TGGAAAAACG

CCAGCAACGC

GGCCTTTTTA

CGGTTCCTGG

CCTTTTGCTG

GCCTTTTGCT

7081

CACATGT

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