名称: | pET28a-6×His-dCas9-20E |
---|---|
别称: | |
基因长: | |
质粒宿主: | 大肠杆菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Kan |
筛选标记: | |
荧光标记: | |
启动子: | T7 |
复制子: | pBR322 |
拷贝数: | 低 |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pET-28a |
正向引物: | T7:TAATACGACTCACTATAGGG |
反向引物: | T7ter:TGCTAGTTATTGCTCAGCGG |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGGCGAATGG
GACGCGCCCT
GTAGCGGCGC
ATTAAGCGCG
GCGGGTGTGG
TGGTTACGCG
CAGCGTGACC
GCTACACTTG
CCAGCGCCCT
AGCGCCCGCT
CCTTTCGCTT
TCTTCCCTTC
CTTTCTCGCC
ACGTTCGCCG
GCTTTCCCCG
TCAAGCTCTA
AATCGGGGGC
TCCCTTTAGG
GTTCCGATTT
AGTGCTTTAC
GGCACCTCGA
CCCCAAAAAA
CTTGATTAGG
GTGATGGTTC
ACGTAGTGGG
CCATCGCCCT
GATAGACGGT
TTTTCGCCCT
TTGACGTTGG
AGTCCACGTT
CTTTAATAGT
GGACTCTTGT
TCCAAACTGG
AACAACACTC
AACCCTATCT
CGGTCTATTC
TTTTGATTTA
TAAGGGATTT
TGCCGATTTC
GGCCTATTGG
TTAAAAAATG
AGCTGATTTA
ACAAAAATTT
AACGCGAATT
TTAACAAAAT
ATTAACGTTT
ACAATTTCAG
GTGGCACTTT
TCGGGGAAAT
GTGCGCGGAA
CCCCTATTTG
TTTATTTTTC
TAAATACATT
CAAATATGTA
TCCGCTCATG
AATTAATTCT
TAGAAAAACT
CATCGAGCAT
CAAATGAAAC
TGCAATTTAT
TCATATCAGG
ATTATCAATA
CCATATTTTT
GAAAAAGCCG
TTTCTGTAAT
GAAGGAGAAA
ACTCACCGAG
GCAGTTCCAT
AGGATGGCAA
GATCCTGGTA
TCGGTCTGCG
ATTCCGACTC
GTCCAACATC
AATACAACCT
ATTAATTTCC
CCTCGTCAAA
AATAAGGTTA
TCAAGTGAGA
AATCACCATG
AGTGACGACT
GAATCCGGTG
AGAATGGCAA
AAGTTTATGC
ATTTCTTTCC
AGACTTGTTC
AACAGGCCAG
CCATTACGCT
CGTCATCAAA
ATCACTCGCA
TCAACCAAAC
CGTTATTCAT
TCGTGATTGC
GCCTGAGCGA
GACGAAATAC
GCGATCGCTG
TTAAAAGGAC
AATTACAAAC
AGGAATCGAA
TGCAACCGGC
GCAGGAACAC
TGCCAGCGCA
TCAACAATAT
TTTCACCTGA
ATCAGGATAT
TCTTCTAATA
CCTGGAATGC
TGTTTTCCCG
GGGATCGCAG
TGGTGAGTAA
CCATGCATCA
TCAGGAGTAC
GGATAAAATG
CTTGATGGTC
GGAAGAGGCA
TAAATTCCGT
CAGCCAGTTT
AGTCTGACCA
TCTCATCTGT
AACATCATTG
GCAACGCTAC
CTTTGCCATG
TTTCAGAAAC
AACTCTGGCG
CATCGGGCTT
CCCATACAAT
CGATAGATTG
TCGCACCTGA
TTGCCCGACA
TTATCGCGAG
CCCATTTATA
CCCATATAAA
TCAGCATCCA
TGTTGGAATT
TAATCGCGGC
CTAGAGCAAG
ACGTTTCCCG
TTGAATATGG
CTCATAACAC
CCCTTGTATT
ACTGTTTATG
TAAGCAGACA
GTTTTATTGT
TCATGACCAA
AATCCCTTAA
CGTGAGTTTT
CGTTCCACTG
AGCGTCAGAC
CCCGTAGAAA
AGATCAAAGG
ATCTTCTTGA
GATCCTTTTT
TTCTGCGCGT
AATCTGCTGC
TTGCAAACAA
AAAAACCACC
GCTACCAGCG
GTGGTTTGTT
TGCCGGATCA
AGAGCTACCA
ACTCTTTTTC
CGAAGGTAAC
TGGCTTCAGC
AGAGCGCAGA
TACCAAATAC
TGTCCTTCTA
GTGTAGCCGT
AGTTAGGCCA
CCACTTCAAG
AACTCTGTAG
CACCGCCTAC
ATACCTCGCT
CTGCTAATCC
TGTTACCAGT
GGCTGCTGCC
AGTGGCGATA
AGTCGTGTCT
TACCGGGTTG
GACTCAAGAC
GATAGTTACC
GGATAAGGCG
CAGCGGTCGG
GCTGAACGGG
GGGTTCGTGC
ACACAGCCCA
GCTTGGAGCG
AACGACCTAC
ACCGAACTGA
GATACCTACA
GCGTGAGCTA
TGAGAAAGCG
CCACGCTTCC
CGAAGGGAGA
AAGGCGGACA
GGTATCCGGT
AAGCGGCAGG
GTCGGAACAG
GAGAGCGCAC
GAGGGAGCTT
CCAGGGGGAA
ACGCCTGGTA
TCTTTATAGT
CCTGTCGGGT
TTCGCCACCT
CTGACTTGAG
CGTCGATTTT
TGTGATGCTC
GTCAGGGGGG
CGGAGCCTAT
GGAAAAACGC
CAGCAACGCG
GCCTTTTTAC
GGTTCCTGGC
CTTTTGCTGG
CCTTTTGCTC
ACATGTTCTT
TCCTGCGTTA
TCCCCTGATT
CTGTGGATAA
CCGTATTACC
GCCTTTGAGT
GAGCTGATAC
CGCTCGCCGC
AGCCGAACGA
CCGAGCGCAG
CGAGTCAGTG
AGCGAGGAAG
CGGAAGAGCG
CCTGATGCGG
TATTTTCTCC
TTACGCATCT
GTGCGGTATT
TCACACCGCA
TATATGGTGC
ACTCTCAGTA
CAATCTGCTC
TGATGCCGCA
TAGTTAAGCC
AGTATACACT
CCGCTATCGC
TACGTGACTG
GGTCATGGCT
GCGCCCCGAC
ACCCGCCAAC
ACCCGCTGAC
GCGCCCTGAC
GGGCTTGTCT
GCTCCCGGCA
TCCGCTTACA
GACAAGCTGT
GACCGTCTCC
GGGAGCTGCA
TGTGTCAGAG
GTTTTCACCG
TCATCACCGA
AACGCGCGAG
GCAGCTGCGG
TAAAGCTCAT
CAGCGTGGTC
GTGAAGCGAT
TCACAGATGT
CTGCCTGTTC
ATCCGCGTCC
AGCTCGTTGA
GTTTCTCCAG
AAGCGTTAAT
GTCTGGCTTC
TGATAAAGCG
GGCCATGTTA
AGGGCGGTTT
TTTCCTGTTT
GGTCACTGAT
GCCTCCGTGT
AAGGGGGATT
TCTGTTCATG
GGGGTAATGA
TACCGATGAA
ACGAGAGAGG
ATGCTCACGA
TACGGGTTAC
TGATGATGAA
CATGCCCGGT
TACTGGAACG
TTGTGAGGGT
AAACAACTGG
CGGTATGGAT
GCGGCGGGAC
CAGAGAAAAA
TCACTCAGGG
TCAATGCCAG
CGCTTCGTTA
ATACAGATGT
AGGTGTTCCA
CAGGGTAGCC
AGCAGCATCC
TGCGATGCAG
ATCCGGAACA
TAATGGTGCA
GGGCGCTGAC
TTCCGCGTTT
CCAGACTTTA
CGAAACACGG
AAACCGAAGA
CCATTCATGT
TGTTGCTCAG
GTCGCAGACG
TTTTGCAGCA
GCAGTCGCTT
CACGTTCGCT
CGCGTATCGG
TGATTCATTC
TGCTAACCAG
TAAGGCAACC
CCGCCAGCCT
AGCCGGGTCC
TCAACGACAG
GAGCACGATC
ATGCGCACCC
GTGGGGCCGC
CATGCCGGCG
ATAATGGCCT
GCTTCTCGCC
GAAACGTTTG
GTGGCGGGAC
CAGTGACGAA
GGCTTGAGCG
AGGGCGTGCA
AGATTCCGAA
TACCGCAAGC
GACAGGCCGA
TCATCGTCGC
GCTCCAGCGA
AAGCGGTCCT
CGCCGAAAAT
GACCCAGAGC
GCTGCCGGCA
CCTGTCCTAC
GAGTTGCATG
ATAAAGAAGA
CAGTCATAAG
TGCGGCGACG
ATAGTCATGC
CCCGCGCCCA
CCGGAAGGAG
CTGACTGGGT
TGAAGGCTCT
CAAGGGCATC
GGTCGAGATC
CCGGTGCCTA
ATGAGTGAGC
TAACTTACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCTGCATT
AATGAATCGG
CCAACGCGCG
GGGAGAGGCG
GTTTGCGTAT
TGGGCGCCAG
GGTGGTTTTT
CTTTTCACCA
GTGAGACGGG
CAACAGCTGA
TTGCCCTTCA
CCGCCTGGCC
CTGAGAGAGT
TGCAGCAAGC
GGTCCACGCT
GGTTTGCCCC
AGCAGGCGAA
AATCCTGTTT
GATGGTGGTT
AACGGCGGGA
TATAACATGA
GCTGTCTTCG
GTATCGTCGT
ATCCCACTAC
CGAGATATCC
GCACCAACGC
GCAGCCCGGA
CTCGGTAATG
GCGCGCATTG
CGCCCAGCGC
CATCTGATCG
TTGGCAACCA
GCATCGCAGT
GGGAACGATG
CCCTCATTCA
GCATTTGCAT
GGTTTGTTGA
AAACCGGACA
TGGCACTCCA
GTCGCCTTCC
CGTTCCGCTA
TCGGCTGAAT
TTGATTGCGA
GTGAGATATT
TATGCCAGCC
AGCCAGACGC
AGACGCGCCG
AGACAGAACT
TAATGGGCCC
GCTAACAGCG
CGATTTGCTG
GTGACCCAAT
GCGACCAGAT
GCTCCACGCC
CAGTCGCGTA
CCGTCTTCAT
GGGAGAAAAT
AATACTGTTG
ATGGGTGTCT
GGTCAGAGAC
ATCAAGAAAT
AACGCCGGAA
CATTAGTGCA
GGCAGCTTCC
ACAGCAATGG
CATCCTGGTC
ATCCAGCGGA
TAGTTAATGA
TCAGCCCACT
GACGCGTTGC
GCGAGAAGAT
TGTGCACCGC
CGCTTTACAG
GCTTCGACGC
CGCTTCGTTC
TACCATCGAC
ACCACCACGC
TGGCACCCAG
TTGATCGGCG
CGAGATTTAA
TCGCCGCGAC
AATTTGCGAC
GGCGCGTGCA
GGGCCAGACT
GGAGGTGGCA
ACGCCAATCA
GCAACGACTG
TTTGCCCGCC
AGTTGTTGTG
CCACGCGGTT
GGGAATGTAA
TTCAGCTCCG
CCATCGCCGC
TTCCACTTTT
TCCCGCGTTT
TCGCAGAAAC
GTGGCTGGCC
TGGTTCACCA
CGCGGGAAAC
GGTCTGATAA
GAGACACCGG
CATACTCTGC
GACATCGTAT
AACGTTACTG
GTTTCACATT
CACCACCCTG
AATTGACTCT
CTTCCGGGCG
CTATCATGCC
ATACCGCGAA
AGGTTTTGCG
CCATTCGATG
GTGTCCGGGA
TCTCGACGCT
CTCCCTTATG
CGACTCCTGC
ATTAGGAAGC
AGCCCAGTAG
TAGGTTGAGG
CCGTTGAGCA
CCGCCGCCGC
AAGGAATGGT
GCATGCAAGG
AGATGGCGCC
CAACAGTCCC
CCGGCCACGG
GGCCTGCCAC
CATACCCACG
CCGAAACAAG
CGCTCATGAG
CCCGAAGTGG
CGAGCCCGAT
CTTCCCCATC
GGTGATGTCG
GCGATATAGG
CGCCAGCAAC
CGCACCTGTG
GCGCCGGTGA
TGCCGGCCAC
GATGCGTCCG
GCGTAGAGGA
TCGAGATCTC
GATCCCGCGA
AATTAATACG
ACTCACTATA
GGGGAATTGT
GAGCGGATAA
CAATTCCCCT
CTAGAAATAA
TTTTGTTTAA
CTTTAAGAAG
GAGATATACC
ATGGACCACC
ACCACCACCA
CCACATGGAT
AAGAAATACT
CAATAGGCTT
AGCTATCGGC
ACAAATAGCG
TCGGATGGGC
GGTGATCACT
GATGAATATA
AGGTTCCGTC
TAAAAAGTTC
AAGGTTCTGG
GAAATACAGA
CCGCCACAGT
ATCAAAAAAA
ATCTTATAGG
GGCTCTTTTA
TTTGACAGTG
GAGAGACAGC
GGAAGCGACT
CGTCTCAAAC
GGACAGCTCG
TAGAAGGTAT
ACACGTCGGA
AGAATCGTAT
TTGTTATCTA
CAGGAGATTT
TTTCAAATGA
GATGGCGAAA
GTAGATGATA
GTTTCTTTCA
TCGACTTGAA
GAGTCTTTTT
TGGTGGAAGA
AGACAAGAAG
CATGAACGTC
ATCCTATTTT
TGGAAATATA
GTAGATGAAG
TTGCTTATCA
TGAGAAATAT
CCAACTATCT
ATCATCTGCG
AAAAAAATTG
GTAGATTCTA
CTGATAAAGC
GGATTTGCGC
TTAATCTATT
TGGCCTTAGC
GCATATGATT
AAGTTTCGTG
GTCATTTTTT
GATTGAGGGA
GATTTAAATC
CTGATAATAG
TGATGTGGAC
AAACTATTTA
TCCAGTTGGT
ACAAACCTAC
AATCAATTAT
TTGAAGAAAA
CCCTATTAAC
GCAAGTGGAG
TAGATGCTAA
AGCGATTCTT
TCTGCACGAT
TGAGTAAATC
AAGACGATTA
GAAAATCTCA
TTGCTCAGCT
CCCCGGTGAG
AAGAAAAATG
GCTTATTTGG
GAATCTCATT
GCTTTGTCAT
TGGGTTTGAC
CCCTAATTTT
AAATCAAATT
TTGATTTGGC
AGAAGATGCT
AAATTACAGC
TTTCAAAAGA
TACTTACGAT
GATGATTTAG
ATAATTTATT
GGCGCAAATT
GGAGATCAAT
ATGCTGATTT
GTTTTTGGCA
GCTAAGAATT
TATCAGATGC
TATTTTACTT
TCAGATATCC
TAAGAGTAAA
TACTGAAATA
ACTAAGGCTC
CCCTATCAGC
TTCAATGATT
AAACGCTACG
ATGAACATCA
TCAAGACTTG
ACTCTTTTAA
AAGCTTTAGT
TCGACAACAA
CTTCCAGAAA
AGTATAAAGA
AATCTTTTTT
GATCAATCAA
AAAACGGATA
TGCAGGTTAT
ATTGATGGGG
GAGCTAGCCA
AGAAGAATTT
TATAAATTTA
TCAAACCAAT
TTTAGAAAAA
ATGGATGGTA
CTGAGGAATT
ATTGGTGAAA
CTAAATCGTG
AAGATTTGCT
GCGCAAGCAA
CGGACCTTTG
ACAACGGCTC
TATTCCCCAT
CAAATTCACT
TGGGTGAGCT
GCATGCTATT
TTGAGAAGAC
AAGAAGACTT
TTATCCATTT
TTAAAAGACA
ATCGTGAGAA
GATTGAAAAA
ATCTTGACTT
TTCGAATTCC
TTATTATGTT
GGTCCATTGG
CGCGTGGCAA
TAGTCGTTTT
GCATGGATGA
CTCGGAAGTC
TGAAGAAACA
ATTACCCCAT
GGAATTTTGA
AGAAGTTGTC
GATAAAGGTG
CTTCAGCTCA
ATCATTTATT
GAACGCATGA
CAAACTTTGA
TAAAAATCTT
CCAAATGAAA
AAGTACTACC
AAAACATAGT
TTGCTTTATG
AGTATTTTAC
GGTTTATAAC
GAATTGACAA
AGGTCAAATA
TGTTACTGAA
GGAATGCGAA
AACCAGCATT
TCTTTCAGGT
GAACAGAAGA
AAGCCATTGT
TGATTTACTC
TTCAAAACAA
ATCGAAAAGT
AACCGTTAAG
CAATTAAAAG
AAGATTATTT
CAAAAAAATA
GAATGTTTTG
ATAGTGTTGA
AATTTCAGGA
GTTGAAGATA
GATTTAATGC
TTCATTAGGT
ACCTACCATG
ATTTGCTAAA
AATTATTAAA
GATAAAGATT
TTTTGGATAA
TGAAGAAAAT
GAAGATATCT
TAGAGGATAT
TGTTTTAACA
TTGACCTTAT
TTGAAGATAG
GGAGATGATT
GAGGAAAGAC
TTAAAACATA
TGCTCACCTC
TTTGATGATA
AGGTGATGAA
ACAGCTTAAA
CGTCGCCGTT
ATACTGGTTG
GGGACGTTTG
TCTCGAAAAT
TGATTAATGG
TATTAGGGAT
AAGCAATCTG
GCAAAACAAT
ATTAGATTTT
TTGAAATCAG
ATGGTTTTGC
CAATCGCAAT
TTTATGCAGC
TGATCCATGA
TGATAGTTTG
ACATTTAAAG
AAGACATTCA
AAAAGCACAA
GTGTCTGGAC
AAGGCGATAG
TTTACATGAA
CATATTGCAA
ATTTAGCTGG
TAGCCCTGCT
ATTAAAAAAG
GTATTTTACA
GACTGTAAAA
GTTGTTGATG
AATTGGTCAA
AGTAATGGGG
CGGCATAAGC
CAGAAAATAT
CGTTATTGAA
ATGGCACGTG
AAAATCAGAC
AACTCAAAAG
GGCCAGAAAA
ATTCGCGAGA
GCGTATGAAA
CGAATCGAAG
AAGGTATCAA
AGAATTAGGA
AGTCAGATTC
TTAAAGAGCA
TCCTGTTGAA
AATACTCAAT
TGCAAAATGA
AAAGCTCTAT
CTCTATTATC
TCCAAAATGG
AAGAGACATG
TATGTGGACC
AAGAATTAGA
TATTAATCGT
TTAAGTGATT
ATGATGTCGA
TGCCATTGTT
CCACAAAGTT
TCCTTAAAGA
CGATTCAATA
GACAATAAGG
TCTTAACGCG
TTCTGATAAA
AATCGTGGTA
AATCGGATAA
CGTTCCAAGT
GAAGAAGTAG
TCAAAAAGAT
GAAAAACTAT
TGGAGACAAC
TTCTAAACGC
CAAGTTAATC
ACTCAACGTA
AGTTTGATAA
TTTAACGAAA
GCTGAACGTG
GAGGTTTGAG
TGAACTTGAT
AAAGCTGGTT
TTATCAAACG
CCAATTGGTT
GAAACTCGCC
AAATCACTAA
GCATGTGGCA
CAAATTTTGG
ATAGTCGCAT
GAATACTAAA
TACGATGAAA
ATGATAAACT
TATTCGAGAG
GTTAAAGTGA
TTACCTTAAA
ATCTAAATTA
GTTTCTGACT
TCCGAAAAGA
TTTCCAATTC
TATAAAGTAC
GTGAGATTAA
CAATTACCAT
CATGCCCATG
ATGCGTATCT
AAATGCCGTC
GTTGGAACTG
CTTTGATTAA
GAAATATCCA
AAACTTGAAT
CGGAGTTTGT
CTATGGTGAT
TATAAAGTTT
ATGATGTTCG
TAAAATGATT
GCTAAGTCTG
AGCAAGAAAT
AGGCAAAGCA
ACCGCAAAAT
ATTTCTTTTA
CTCTAATATC
ATGAACTTCT
TCAAAACAGA
AATTACACTT
GCAAATGGAG
AGATTCGCAA
ACGCCCTCTA
ATCGAAACTA
ATGGGGAAAC
TGGAGAAATT
GTCTGGGATA
AAGGGCGAGA
TTTTGCCACA
GTGCGCAAAG
TATTGTCCAT
GCCCCAAGTC
AATATTGTCA
AGAAAACAGA
AGTACAGACA
GGCGGATTCT
CCAAGGAGTC
AATTTTACCA
AAAAGAAATT
CGGACAAGCT
TATTGCTCGT
AAAAAAGACT
GGGATCCAAA
AAAATATGGT
GGTTTTGATA
GTCCAACGGT
AGCTTATTCA
GTCCTAGTGG
TTGCTAAGGT
GGAAAAAGGG
AAATCGAAGA
AGTTAAAATC
CGTTAAAGAG
TTACTAGGGA
TCACAATTAT
GGAAAGAAGT
TCCTTTGAAA
AAAATCCGAT
TGACTTTTTA
GAAGCTAAAG
GATATAAGGA
AGTTAAAAAA
GACTTAATCA
TTAAACTACC
TAAATATAGT
CTTTTTGAGT
TAGAAAACGG
TCGTAAACGG
ATGCTGGCTA
GTGCCGGAGA
ATTACAAAAA
GGAAATGAGC
TGGCTCTGCC
AAGCAAATAT
GTGAATTTTT
TATATTTAGC
TAGTCATTAT
GAAAAGTTGA
AGGGTAGTCC
AGAAGATAAC
GAACAAAAAC
AATTGTTTGT
GGAGCAGCAT
AAGCATTATT
TAGATGAGAT
TATTGAGCAA
ATCAGTGAAT
TTTCTAAGCG
TGTTATTTTA
GCAGATGCCA
ATTTAGATAA
AGTTCTTAGT
GCATATAACA
AACATAGAGA
CAAACCAATA
CGTGAACAAG
CAGAAAATAT
TATTCATTTA
TTTACGTTGA
CGAATCTTGG
AGCTCCCGCT
GCTTTTAAAT
ATTTTGATAC
AACAATTGAT
CGTAAACGAT
ATACGTCTAC
AAAAGAAGTT
TTAGATGCCA
CTCTTATCCA
TCAATCCATC
ACTGGTCTTT
ATGAAACACG
CATTGATTTG
AGTCAGCTAG
GAGGTGACAA
GCGACCTGCC
GCCACAAAGA
AGGCTGGACA
GGCTAAGAAG
AAGAAAATGG
AAGAAGAGGA
GGAAGAAGAG
GAGGAAGAAG
AGGAAGAAGA
GGAAGAGGAG
GAGGAAGAGT
AATTTTTAAT
CTCGAGCACC
ACCACCACCA
CCACTGAGAT
CCGGCTGCTA
ACAAAGCCCG
AAAGGAAGCT
GAGTTGGCTG
CTGCCACCGC
TGAGCAATAA
CTAGCATAAC
CCCTTGGGGC
CTCTAAACGG
GTCTTGAGGG
GTTTTTTGCT
GAAAGGAGGA
ACTATATCCG
GAT