名称: | dCas9-VPR-EGFP-1 |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | Zeo |
荧光标记: | 绿色 |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 30度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTCGACGGAT
CGGGAGATCT
CCCGATCCCC
TATGGTGCAC
TCTCAGTACA
ATCTGCTCTG
ATGCCGCATA
GTTAAGCCAG
TATCTGCTCC
CTGCTTGTGT
GTTGGAGGTC
GCTGAGTAGT
GCGCGAGCAA
AATTTAAGCT
ACAACAAGGC
AAGGCTTGAC
CGACAATTGC
ATGAAGAATC
TGCTTAGGGT
TAGGCGTTTT
GCGCTGCTTC
GCGATGTACG
GGCCAGATAT
ACGCGTTGAC
ATTGATTATT
GACTAGTTAT
TAATAGTAAT
CAATTACGGG
GTCATTAGTT
CATAGCCCAT
ATATGGAGTT
CCGCGTTACA
TAACTTACGG
TAAATGGCCC
GCCTGGCTGA
CCGCCCAACG
ACCCCCGCCC
ATTGACGTCA
ATAATGACGT
ATGTTCCCAT
AGTAACGCCA
ATAGGGACTT
TCCATTGACG
TCAATGGGTG
GAGTATTTAC
GGTAAACTGC
CCACTTGGCA
GTACATCAAG
TGTATCATAT
GCCAAGTACG
CCCCCTATTG
ACGTCAATGA
CGGTAAATGG
CCCGCCTGGC
ATTATGCCCA
GTACATGACC
TTATGGGACT
TTCCTACTTG
GCAGTACATC
TACGTATTAG
TCATCGCTAT
TACCATGGTG
ATGCGGTTTT
GGCAGTACAT
CAATGGGCGT
GGATAGCGGT
TTGACTCACG
GGGATTTCCA
AGTCTCCACC
CCATTGACGT
CAATGGGAGT
TTGTTTTGGC
ACCAAAATCA
ACGGGACTTT
CCAAAATGTC
GTAACAACTC
CGCCCCATTG
ACGCAAATGG
GCGGTAGGCG
TGTACGGTGG
GAGGTCTATA
TAAGCAGCGC
GTTTTGCCTG
TACTGGGTCT
CTCTGGTTAG
ACCAGATCTG
AGCCTGGGAG
CTCTCTGGCT
AACTAGGGAA
CCCACTGCTT
AAGCCTCAAT
AAAGCTTGCC
TTGAGTGCTT
CAAGTAGTGT
GTGCCCGTCT
GTTGTGTGAC
TCTGGTAACT
AGAGATCCCT
CAGACCCTTT
TAGTCAGTGT
GGAAAATCTC
TAGCAGTGGC
GCCCGAACAG
GGACTTGAAA
GCGAAAGGGA
AACCAGAGGA
GCTCTCTCGA
CGCAGGACTC
GGCTTGCTGA
AGCGCGCACG
GCAAGAGGCG
AGGGGCGGCG
ACTGGTGAGT
ACGCCAAAAA
TTTTGACTAG
CGGAGGCTAG
AAGGAGAGAG
ATGGGTGCGA
GAGCGTCAGT
ATTAAGCGGG
GGAGAATTAG
ATCGCGATGG
GAAAAAATTC
GGTTAAGGCC
AGGGGGAAAG
AAAAAATATA
AATTAAAACA
TATAGTATGG
GCAAGCAGGG
AGCTAGAACG
ATTCGCAGTT
AATCCTGGCC
TGTTAGAAAC
ATCAGAAGGC
TGTAGACAAA
TACTGGGACA
GCTACAACCA
TCCCTTCAGA
CAGGATCAGA
AGAACTTAGA
TCATTATATA
ATACAGTAGC
AACCCTCTAT
TGTGTGCATC
AAAGGATAGA
GATAAAAGAC
ACCAAGGAAG
CTTTAGACAA
GATAGAGGAA
GAGCAAAACA
AAAGTAAGAC
CACCGCACAG
CAAGCGGCCG
CTGATCTTCA
GACCTGGAGG
AGGAGATATG
AGGGACAATT
GGAGAAGTGA
ATTATATAAA
TATAAAGTAG
TAAAAATTGA
ACCATTAGGA
GTAGCACCCA
CCAAGGCAAA
GAGAAGAGTG
GTGCAGAGAG
AAAAAAGAGC
AGTGGGAATA
GGAGCTTTGT
TCCTTGGGTT
CTTGGGAGCA
GCAGGAAGCA
CTATGGGCGC
AGCGTCAATG
ACGCTGACGG
TACAGGCCAG
ACAATTATTG
TCTGGTATAG
TGCAGCAGCA
GAACAATTTG
CTGAGGGCTA
TTGAGGCGCA
ACAGCATCTG
TTGCAACTCA
CAGTCTGGGG
CATCAAGCAG
CTCCAGGCAA
GAATCCTGGC
TGTGGAAAGA
TACCTAAAGG
ATCAACAGCT
CCTGGGGATT
TGGGGTTGCT
CTGGAAAACT
CATTTGCACC
ACTGCTGTGC
CTTGGAATGC
TAGTTGGAGT
AATAAATCTC
TGGAACAGAT
TTGGAATCAC
ACGACCTGGA
TGGAGTGGGA
CAGAGAAATT
AACAATTACA
CAAGCTTAAT
ACACTCCTTA
ATTGAAGAAT
CGCAAAACCA
GCAAGAAAAG
AATGAACAAG
AATTATTGGA
ATTAGATAAA
TGGGCAAGTT
TGTGGAATTG
GTTTAACATA
ACAAATTGGC
TGTGGTATAT
AAAATTATTC
ATAATGATAG
TAGGAGGCTT
GGTAGGTTTA
AGAATAGTTT
TTGCTGTACT
TTCTATAGTG
AATAGAGTTA
GGCAGGGATA
TTCACCATTA
TCGTTTCAGA
CCCACCTCCC
AACCCCGAGG
GGACCCGACA
GGCCCGAAGG
AATAGAAGAA
GAAGGTGGAG
AGAGAGACAG
AGACAGATCC
ATTCGATTAG
TGAACGGATC
GGCACTGCGT
GCGCCAATTC
TGCAGACAAA
TGGCAGTATT
CATCCACAAT
TTTAAAAGAA
AAGGGGGGAT
TGGGGGGTAC
AGTGCAGGGG
AAAGAATAGT
AGACATAATA
GCAACAGACA
TACAAACTAA
AGAATTACAA
AAACAAATTA
CAAAAATTCA
AAATTTTCGG
GTTTATTACA
GGGACAGCAG
AGATCCAGTT
TGGTTAATTA
GCTAGCTGCA
AAGATGGATA
AAGTTTTAAA
CAGAGAGGAA
TCTTTGCAGC
TAATGGACCT
TCTAGGTCTT
GAAAGGAGTG
GGAATTGGCT
CCGGTGCCCG
TCAGTGGGCA
GAGCGCACAT
CGCCCACAGT
CCCCGAGAAG
TTGGGGGGAG
GGGTCGGCAA
TTGAACCGGT
GCCTAGAGAA
GGTGGCGCGG
GGTAAACTGG
GAAAGTGATG
TCGTGTACTG
GCTCCGCCTT
TTTCCCGAGG
GTGGGGGAGA
ACCGTATATA
AGTGCAGTAG
TCGCCGTGAA
CGTTCTTTTT
CGCAACGGGT
TTGCCGCCAG
AACACAGGTA
AGTGCCGTGT
GTGGTTCCCG
CGGGCCTGGC
CTCTTTACGG
GTTATGGCCC
TTGCGTGCCT
TGAATTACTT
CCACCTGGCT
GCAGTACGTG
ATTCTTGATC
CCGAGCTTCG
GGTTGGAAGT
GGGTGGGAGA
GTTCGAGGCC
TTGCGCTTAA
GGAGCCCCTT
CGCCTCGTGC
TTGAGTTGAG
GCCTGGCCTG
GGCGCTGGGG
CCGCCGCGTG
CGAATCTGGT
GGCACCTTCG
CGCCTGTCTC
GCTGCTTTCG
ATAAGTCTCT
AGCCATTTAA
AATTTTTGAT
GACCTGCTGC
GACGCTTTTT
TTCTGGCAAG
ATAGTCTTGT
AAATGCGGGC
CAAGATCTGC
ACACTGGTAT
TTCGGTTTTT
GGGGCCGCGG
GCGGCGACGG
GGCCCGTGCG
TCCCAGCGCA
CATGTTCGGC
GAGGCGGGGC
CTGCGAGCGC
GGCCACCGAG
AATCGGACGG
GGGTAGTCTC
AAGCTGGCCG
GCCTGCTCTG
GTGCCTGGCC
TCGCGCCGCC
GTGTATCGCC
CCGCCCTGGG
CGGCAAGGCT
GGCCCGGTCG
GCACCAGTTG
CGTGAGCGGA
AAGATGGCCG
CTTCCCGGCC
CTGCTGCAGG
GAGCTCAAAA
TGGAGGACGC
GGCGCTCGGG
AGAGCGGGCG
GGTGAGTCAC
CCACACAAAG
GAAAAGGGCC
TTTCCGTCCT
CAGCCGTCGC
TTCATGTGAC
TCCACGGAGT
ACCGGGCGCC
GTCCAGGCAC
CTCGATTAGT
TCTCGAGCTT
TTGGAGTACG
TCGTCTTTAG
GTTGGGGGGA
GGGGTTTTAT
GCGATGGAGT
TTCCCCACAC
TGAGTGGGTG
GAGACTGAAG
TTAGGCCAGC
TTGGCACTTG
ATGTAATTCT
CCTTGGAATT
TGCCCTTTTT
GAGTTTGGAT
CTTGGTTCAT
TCTCAAGCCT
CAGACAGTGG
TTCAAAGTTT
TTTTCTTCCA
TTTCAGGTGT
CGTGACGTAC
GGCCACCATG
AGCCCCAAGA
AGAAGAGAAA
GGTGGAGGCC
AGCGACAAGA
AGTACAGCAT
CGGCCTGGCC
ATCGGCACCA
ACTCTGTGGG
CTGGGCCGTG
ATCACCGACG
AGTACAAGGT
GCCCAGCAAG
AAATTCAAGG
TGCTGGGCAA
CACCGACCGG
CACAGCATCA
AGAAGAACCT
GATCGGAGCC
CTGCTGTTCG
ACAGCGGCGA
AACAGCCGAG
GCCACCCGGC
TGAAGAGAAC
CGCCAGAAGA
AGATACACCA
GACGGAAGAA
CCGGATCTGC
TATCTGCAAG
AGATCTTCAG
CAACGAGATG
GCCAAGGTGG
ACGACAGCTT
CTTCCACAGA
CTGGAAGAGT
CCTTCCTGGT
GGAAGAGGAT
AAGAAGCACG
AGCGGCACCC
CATCTTCGGC
AACATCGTGG
ACGAGGTGGC
CTACCACGAG
AAGTACCCCA
CCATCTACCA
CCTGAGAAAG
AAACTGGTGG
ACAGCACCGA
CAAGGCCGAC
CTGCGGCTGA
TCTATCTGGC
CCTGGCCCAC
ATGATCAAGT
TCCGGGGCCA
CTTCCTGATC
GAGGGCGACC
TGAACCCCGA
CAACAGCGAC
GTGGACAAGC
TGTTCATCCA
GCTGGTGCAG
ACCTACAACC
AGCTGTTCGA
GGAAAACCCC
ATCAACGCCA
GCGGCGTGGA
CGCCAAGGCC
ATCCTGTCTG
CCAGACTGAG
CAAGAGCAGA
CGGCTGGAAA
ATCTGATCGC
CCAGCTGCCC
GGCGAGAAGA
AGAATGGCCT
GTTCGGCAAC
CTGATTGCCC
TGAGCCTGGG
CCTGACCCCC
AACTTCAAGA
GCAACTTCGA
CCTGGCCGAG
GATGCCAAAC
TGCAGCTGAG
CAAGGACACC
TACGACGACG
ACCTGGACAA
CCTGCTGGCC
CAGATCGGCG
ACCAGTACGC
CGACCTGTTT
CTGGCCGCCA
AGAACCTGTC
CGACGCCATC
CTGCTGAGCG
ACATCCTGAG
AGTGAACACC
GAGATCACCA
AGGCCCCCCT
GAGCGCCTCT
ATGATCAAGA
GATACGACGA
GCACCACCAG
GACCTGACCC
TGCTGAAAGC
TCTCGTGCGG
CAGCAGCTGC
CTGAGAAGTA
CAAAGAGATT
TTCTTCGACC
AGAGCAAGAA
CGGCTACGCC
GGCTACATTG
ACGGCGGAGC
CAGCCAGGAA
GAGTTCTACA
AGTTCATCAA
GCCCATCCTG
GAAAAGATGG
ACGGCACCGA
GGAACTGCTC
GTGAAGCTGA
ACAGAGAGGA
CCTGCTGCGG
AAGCAGCGGA
CCTTCGACAA
CGGCAGCATC
CCCCACCAGA
TCCACCTGGG
AGAGCTGCAC
GCCATTCTGC
GGCGGCAGGA
AGATTTTTAC
CCATTCCTGA
AGGACAACCG
GGAAAAGATC
GAGAAGATCC
TGACCTTCCG
CATCCCCTAC
TACGTGGGCC
CTCTGGCCAG
GGGAAACAGC
AGATTCGCCT
GGATGACCAG
AAAGAGCGAG
GAAACCATCA
CCCCCTGGAA
CTTCGAGGAA
GTGGTGGACA
AGGGCGCTTC
CGCCCAGAGC
TTCATCGAGC
GGATGACCAA
CTTCGATAAG
AACCTGCCCA
ACGAGAAGGT
GCTGCCCAAG
CACAGCCTGC
TGTACGAGTA
CTTCACCGTG
TATAACGAGC
TGACCAAAGT
GAAATACGTG
ACCGAGGGAA
TGAGAAAGCC
CGCCTTCCTG
AGCGGCGAGC
AGAAAAAGGC
CATCGTGGAC
CTGCTGTTCA
AGACCAACCG
GAAAGTGACC
GTGAAGCAGC
TGAAAGAGGA
CTACTTCAAG
AAAATCGAGT
GCTTCGACTC
CGTGGAAATC
TCCGGCGTGG
AAGATCGGTT
CAACGCCTCC
CTGGGCACAT
ACCACGATCT
GCTGAAAATT
ATCAAGGACA
AGGACTTCCT
GGACAATGAG
GAAAACGAGG
ACATTCTGGA
AGATATCGTG
CTGACCCTGA
CACTGTTTGA
GGACAGAGAG
ATGATCGAGG
AACGGCTGAA
AACCTATGCC
CACCTGTTCG
ACGACAAAGT
GATGAAGCAG
CTGAAGCGGC
GGAGATACAC
CGGCTGGGGC
AGGCTGAGCC
GGAAGCTGAT
CAACGGCATC
CGGGACAAGC
AGTCCGGCAA
GACAATCCTG
GATTTCCTGA
AGTCCGACGG
CTTCGCCAAC
AGAAACTTCT
CCGGACTCAG
ATCTGGCAGC
GGTCTCGAGA
TGGAAGTTAG
CGCTCTGGAA
AAAGAAGTGT
CTGCACTCGA
GAAAGAAGTA
AGTGCCCTTG
AGAAGGAGGT
GTCCGCACTC
GAGAAGGAGG
TCAGCGCCCT
GGAAAAGGGA
GGCGGAGGCT
CCATGCAGCT
GATCCACGAC
GACAGCCTGA
CCTTTAAAGA
GGACATCCAG
AAAGCCCAGG
TGGAAAACCT
GTACTTTCAG
TCCCCATCAT
CAAAGGTGTC
CGCCCTGAAG
GAAAAAGTAA
GCGCACTGAA
AGAAAAGGTG
AGCGCGCTGA
AGGAGAAAGT
GAGCGCCCTG
AAAGAGAAAG
TCTCTGCCCT
TAAGGAGGGA
GGCGGAGGCT
CCTCCGGCCA
GGGCGATAGC
CTGCACGAGC
ACATTGCCAA
TCTGGCCGGC
AGCCCCGCCA
TTAAGAAGGG
CATCCTGCAG
ACAGTGAAGG
TGGTGGACGA
GCTCGTGAAA
GTGATGGGCC
GGCACAAGCC
CGAGAACATC
GTGATCGAAA
TGGCCAGAGA
GAACCAGACC
ACCCAGAAGG
GACAGAAGAA
CAGCCGCGAG
AGAATGAAGC
GGATCGAAGA
GGGCATCAAA
GAGCTGGGCA
GCCAGATCCT
GAAAGAACAC
CCCGTGGAAA
ACACCCAGCT
GCAGAACGAG
AAGCTGTACC
TGTACTACCT
GCAGAATGGG
CGGGATATGT
ACGTGGACCA
GGAACTGGAC
ATCAACCGGC
TGTCCGACTA
CGATGTGGAC
GCTATCGTGC
CTCAGAGCTT
TCTGAAGGAC
GACTCCATCG
ACAACAAGGT
GCTGACCAGA
AGCGACAAGA
ACCGGGGCAA
GAGCGACAAC
GTGCCCTCCG
AAGAGGTCGT
GAAGAAGATG
AAGAACTACT
GGCGGCAGCT
GCTGAACGCC
AAGCTGATTA
CCCAGAGAAA
GTTCGACAAT
CTGACCAAGG
CCGAGAGAGG
CGGCCTGAGC
GAACTGGATA
AGGCCGGCTT
CATCAAGAGA
CAGCTGGTGG
AAACCCGGCA
GATCACAAAG
CACGTGGCAC
AGATCCTGGA
CTCCCGGATG
AACACTAAGT
ACGACGAGAA
TGACAAGCTG
ATCCGGGAAG
TGAAAGTGAT
CACCCTGAAG
TCCAAGCTGG
TGTCCGATTT
CCGGAAGGAT
TTCCAGTTTT
ACAAAGTGCG
CGAGATCAAC
AACTACCACC
ACGCCCACGA
CGCCTACCTG
AACGCCGTCG
TGGGAACCGC
CCTGATCAAA
AAGTACCCTA
AGCTGGAAAG
CGAGTTCGTG
TACGGCGACT
ACAAGGTGTA
CGACGTGCGG
AAGATGATCG
CCAAGAGCGA
GCAGGAAATC
GGCAAGGCTA
CCGCCAAGTA
CTTCTTCTAC
AGCAACATCA
TGAACTTTTT
CAAGACCGAG
ATTACCCTGG
CCAACGGCGA
GATCCGGAAG
CGGCCTCTGA
TCGAGACAAA
CGGCGAAACC
GGGGAGATCG
TGTGGGATAA
GGGCCGGGAT
TTTGCCACCG
TGCGGAAAGT
GCTGAGCATG
CCCCAAGTGA
ATATCGTGAA
AAAGACCGAG
GTGCAGACAG
GCGGCTTCAG
CAAAGAGTCT
ATCCTGCCCA
AGAGGAACAG
CGATAAGCTG
ATCGCCAGAA
AGAAGGACTG
GGACCCTAAG
AAGTACGGCG
GCTTCGACAG
CCCCACCGTG
GCCTATTCTG
TGCTGGTGGT
GGCCAAAGTG
GAAAAGGGCA
AGTCCAAGAA
ACTGAAGAGT
GTGAAAGAGC
TGCTGGGGAT
CACCATCATG
GAAAGAAGCA
GCTTCGAGAA
GAATCCCATC
GACTTTCTGG
AAGCCAAGGG
CTACAAAGAA
GTGAAAAAGG
ACCTGATCAT
CAAGCTGCCT
AAGTACTCCC
TGTTCGAGCT
GGAAAACGGC
CGGAAGAGAA
TGCTGGCCTC
TGCCGGCGAA
CTGCAGAAGG
GAAACGAACT
GGCCCTGCCC
TCCAAATATG
TGAACTTCCT
GTACCTGGCC
AGCCACTATG
AGAAGCTGAA
GGGCTCCCCC
GAGGATAATG
AGCAGAAACA
GCTGTTTGTG
GAACAGCACA
AGCACTACCT
GGACGAGATC
ATCGAGCAGA
TCAGCGAGTT
CTCCAAGAGA
GTGATCCTGG
CCGACGCTAA
TCTGGACAAA
GTGCTGTCCG
CCTACAACAA
GCACCGGGAT
AAGCCCATCA
GAGAGCAGGC
CGAGAATATC
ATCCACCTGT
TTACCCTGAC
CAATCTGGGA
GCCCCTGCCG
CCTTCAAGTA
CTTTGACACC
ACCATCGACC
GGAAGAGGTA
CACCAGCACC
AAAGAGGTGC
TGGACGCCAC
CCTGATCCAC
CAGAGCATCA
CCGGCCTGTA
CGAGACACGG
ATCGACCTGT
CTCAGCTGGG
AGGCGACAGC
GCTGGAGGAG
GTGGAAGCGG
AGGAGGAGGA
AGCGGAGGAG
GAGGTAGCGG
ACCTAAGAAA
AAGAGGAAGG
TGGCGGCCGC
TGGATCCGGA
CGGGCTGACG
CATTGGACGA
TTTTGATCTG
GATATGCTGG
GAAGTGACGC
CCTCGATGAT
TTTGACCTTG
ACATGCTTGG
TTCGGATGCC
CTTGATGACT
TTGACCTCGA
CATGCTCGGC
AGTGACGCCC
TTGATGATTT
CGACCTGGAC
ATGCTGATTA
ACGCTAGCGG
CAGTGGACCT
ACACAGGCCG
GCGAGGGCAC
ACTGTCTGAA
GCTCTGCTGC
AGCTGCAGTT
CGACGACGAG
GATCTGGGAG
CCCTGCTGGG
AAACAGCACC
GATCCTGCCG
TGTTCACCGA
CCTGGCCAGC
GTGGACAACA
GCGAGTTCCA
GCAGCTGCTG
AACCAGGGCA
TCCCTGTGGC
CCCTCACACC
ACCGAGCCCA
TGCTGATGGA
ATACCCCGAG
GCCATCACCC
GGCTCGTGAC
AGGCGCTCAG
AGGCCTCCTG
ATCCAGCTCC
TGCCCCTCTG
GGAGCACCAG
GCCTGCCTAA
TGGACTGCTG
TCTGGCGACG
AGGACTTCAG
CTCTATCGCC
GATATGGATT
TCTCAGCCTT
GCTGGGCTCT
GGCAGCGGCA
GCCGGGATTC
CAGGGAAGGG
ATGTTTTTGC
CGAAGCCTGA
GGCCGGCTCC
GCTATTAGTG
ACGTGTTTGA
GGGCCGCGAG
GTGTGCCAGC
CAAAACGAAT
CCGGCCATTT
CATCCTCCAG
GAAGTCCATG
GGCCAACCGC
CCACTCCCCG
CCAGCCTCGC
ACCAACACCA
ACCGGTCCAG
TACATGAGCC
AGTCGGGTCA
CTGACCCCGG
CACCAGTCCC
TCAGCCACTG
GATCCAGCGC
CCGCAGTGAC
TCCCGAGGCC
AGTCACCTGT
TGGAGGATCC
CGATGAAGAG
ACGAGCCAGG
CTGTCAAAGC
CCTTCGGGAG
ATGGCCGATA
CTGTGATTCC
CCAGAAGGAA
GAGGCTGCAA
TCTGTGGCCA
AATGGACCTT
TCCCATCCGC
CCCCAAGGGG
CCATCTGGAT
GAGCTGACAA
CCACACTTGA
GTCCATGACC
GAGGATCTGA
ACCTGGACTC
ACCCCTGACC
CCGGAATTGA
ACGAGATTCT
GGATACCTTC
CTGAACGACG
AGTGCCTCTT
GCATGCCATG
CATATCAGCA
CAGGACTGTC
CATCTTCGAC
ACATCTCTCT
TTGAGGGCAG
AGGAAGTCTG
CTAACATGCG
GTGACGTCGA
GGAGAATCCT
GGCCCAGTGA
GCAAGGGCGA
GGAGCTGTTC
ACCGGGGTGG
TGCCCATCCT
GGTCGAGCTG
GACGGCGACG
TAAACGGCCA
CAAGTTCAGC
GTGTCCGGCG
AGGGCGAGGG
CGATGCCACC
TACGGCAAGC
TGACCCTGAA
GTTCATCTGC
ACCACCGGCA
AGCTGCCCGT
GCCCTGGCCC
ACCCTCGTGA
CCACCCTGAC
CTACGGCGTG
CAGTGCTTCA
GCCGCTACCC
CGACCACATG
AAGCAGCACG
ACTTCTTCAA
GTCCGCCATG
CCCGAAGGCT
ACGTCCAGGA
GCGCACCATC
TTCTTCAAGG
ACGACGGCAA
CTACAAGACC
CGCGCCGAGG
TGAAGTTCGA
GGGCGACACC
CTGGTGAACC
GCATCGAGCT
GAAGGGCATC
GACTTCAAGG
AGGACGGCAA
CATCCTGGGG
CACAAGCTGG
AGTACAACTA
CAACAGCCAC
AACGTCTATA
TCATGGCCGA
CAAGCAGAAG
AACGGCATCA
AGGTGAACTT
CAAGATCCGC
CACAACATCG
AGGACGGCAG
CGTGCAGCTC
GCCGACCACT
ACCAGCAGAA
CACCCCCATC
GGCGACGGCC
CCGTGCTGCT
GCCCGACAAC
CACTACCTGA
GCACCCAGTC
CGCCCTGAGC
AAAGACCCCA
ACGAGAAGCG
CGATCACATG
GTCCTGCTGG
AGTTCGTGAC
CGCCGCCGGG
ATCACTCTCG
GCATGGACGA
GCTGTACAAG
TAAGAATTCG
ATATCAAGCT
TATCGGTAAT
CAACCTCTGG
ATTACAAAAT
TTGTGAAAGA
TTGACTGGTA
TTCTTAACTA
TGTTGCTCCT
TTTACGCTAT
GTGGATACGC
TGCTTTAATG
CCTTTGTATC
ATGCTATTGC
TTCCCGTATG
GCTTTCATTT
TCTCCTCCTT
GTATAAATCC
TGGTTGCTGT
CTCTTTATGA
GGAGTTGTGG
CCCGTTGTCA
GGCAACGTGG
CGTGGTGTGC
ACTGTGTTTG
CTGACGCAAC
CCCCACTGGT
TGGGGCATTG
CCACCACCTG
TCAGCTCCTT
TCCGGGACTT
TCGCTTTCCC
CCTCCCTATT
GCCACGGCGG
AACTCATCGC
CGCCTGCCTT
GCCCGCTGCT
GGACAGGGGC
TCGGCTGTTG
GGCACTGACA
ATTCCGTGGT
GTTGTCGGGG
AAATCATCGT
CCTTTCCTTG
GCTGCTCGCC
TGTGTTGCCA
CCTGGATTCT
GCGCGGGACG
TCCTTCTGCT
ACGTCCCTTC
GGCCCTCAAT
CCAGCGGACC
TTCCTTCCCG
CGGCCTGCTG
CCGGCTCTGC
GGCCTCTTCC
GCGTCTTCGC
CTTCGCCCTC
AGACGAGTCG
GATCTCCCTT
TGGGCCGCCT
CCCCGCATCG
ATACCGTCGA
CCTCGAGACC
TAGAAAAACA
TGGAGCAATC
ACAAGTAGCA
ATACAGCAGC
TACCAATGCT
GATTGTGCCT
GGCTAGAAGC
ACAAGAGGAG
GAGGAGGTGG
GTTTTCCAGT
CACACCTCAG
GTACCTTTAA
GACCAATGAC
TTACAAGGCA
GCTGTAGATC
TTAGCCACTT
TTTAAAAGAA
AAGGGGGGAC
TGGAAGGGCT
AATTCACTCC
CAACGAAGAC
AAGATATCCT
TGATCTGTGG
ATCTACCACA
CACAAGGCTA
CTTCCCTGAT
TGGCAGAACT
ACACACCAGG
GCCAGGGATC
AGATATCCAC
TGACCTTTGG
ATGGTGCTAC
AAGCTAGTAC
CAGTTGAGCA
AGAGAAGGTA
GAAGAAGCCA
ATGAAGGAGA
GAACACCCGC
TTGTTACACC
CTGTGAGCCT
GCATGGGATG
GATGACCCGG
AGAGAGAAGT
ATTAGAGTGG
AGGTTTGACA
GCCGCCTAGC
ATTTCATCAC
ATGGCCCGAG
AGCTGCATCC
GGACTGTACT
GGGTCTCTCT
GGTTAGACCA
GATCTGAGCC
TGGGAGCTCT
CTGGCTAACT
AGGGAACCCA
CTGCTTAAGC
CTCAATAAAG
CTTGCCTTGA
GTGCTTCAAG
TAGTGTGTGC
CCGTCTGTTG
TGTGACTCTG
GTAACTAGAG
ATCCCTCAGA
CCCTTTTAGT
CAGTGTGGAA
AATCTCTAGC
AGGGCCCGTT
TAAACCCGCT
GATCAGCCTC
GACTGTGCCT
TCTAGTTGCC
AGCCATCTGT
TGTTTGCCCC
TCCCCCGTGC
CTTCCTTGAC
CCTGGAAGGT
GCCACTCCCA
CTGTCCTTTC
CTAATAAAAT
GAGGAAATTG
CATCGCATTG
TCTGAGTAGG
TGTCATTCTA
TTCTGGGGGG
TGGGGTGGGG
CAGGACAGCA
AGGGGGAGGA
TTGGGAAGAC
AATAGCAGGC
ATGCTGGGGA
TGCGGTGGGC
TCTATGGCTT
CTGAGGCGGA
AAGAACCAGC
TGGGGCTCTA
GGGGGTATCC
CCACGCGCCC
TGTAGCGGCG
CATTAAGCGC
GGCGGGTGTG
GTGGTTACGC
GCAGCGTGAC
CGCTACACTT
GCCAGCGCCC
TAGCGCCCGC
TCCTTTCGCT
TTCTTCCCTT
CCTTTCTCGC
CACGTTCGCC
GGCTTTCCCC
GTCAAGCTCT
AAATCGGGGG
CTCCCTTTAG
GGTTCCGATT
TAGTGCTTTA
CGGCACCTCG
ACCCCAAAAA
ACTTGATTAG
GGTGATGGTT
CACGTAGTGG
GCCATCGCCC
TGATAGACGG
TTTTTCGCCC
TTTGACGTTG
GAGTCCACGT
TCTTTAATAG
TGGACTCTTG
TTCCAAACTG
GAACAACACT
CAACCCTATC
TCGGTCTATT
CTTTTGATTT
ATAAGGGATT
TTGCCGATTT
CGGCCTATTG
GTTAAAAAAT
GAGCTGATTT
AACAAAAATT
TAACGCGAAT
TAATTCTGTG
GAATGTGTGT
CAGTTAGGGT
GTGGAAAGTC
CCCAGGCTCC
CCAGCAGGCA
GAAGTATGCA
AAGCATGCAT
CTCAATTAGT
CAGCAACCAG
GTGTGGAAAG
TCCCCAGGCT
CCCCAGCAGG
CAGAAGTATG
CAAAGCATGC
ATCTCAATTA
GTCAGCAACC
ATAGTCCCGC
CCCTAACTCC
GCCCATCCCG
CCCCTAACTC
CGCCCAGTTC
CGCCCATTCT
CCGCCCCATG
GCTGACTAAT
TTTTTTTATT
TATGCAGAGG
CCGAGGCCGC
CTCTGCCTCT
GAGCTATTCC
AGAAGTAGTG
AGGAGGCTTT
TTTGGAGGCC
TAGGCTTTTG
CAAAAAGCTC
CCGGGAGCTT
GTATATCCAT
TTTCGGATCT
GATCAGCACG
TGTTGACAAT
TAATCATCGG
CATAGTATAT
CGGCATAGTA
TAATACGACA
AGGTGAGGAA
CTAAACCATG
GCCAAGTTGA
CCAGTGCCGT
TCCGGTGCTC
ACCGCGCGCG
ACGTCGCCGG
AGCGGTCGAG
TTCTGGACCG
ACCGGCTCGG
GTTCTCCCGG
GACTTCGTGG
AGGACGACTT
CGCCGGTGTG
GTCCGGGACG
ACGTGACCCT
GTTCATCAGC
GCGGTCCAGG
ACCAGGTGGT
GCCGGACAAC
ACCCTGGCCT
GGGTGTGGGT
GCGCGGCCTG
GACGAGCTGT
ACGCCGAGTG
GTCGGAGGTC
GTGTCCACGA
ACTTCCGGGA
CGCCTCCGGG
CCGGCCATGA
CCGAGATCGG
CGAGCAGCCG
TGGGGGCGGG
AGTTCGCCCT
GCGCGACCCG
GCCGGCAACT
GCGTGCACTT
CGTGGCCGAG
GAGCAGGACT
GACACGTGCT
ACGAGATTTC
GATTCCACCG
CCGCCTTCTA
TGAAAGGTTG
GGCTTCGGAA
TCGTTTTCCG
GGACGCCGGC
TGGATGATCC
TCCAGCGCGG
GGATCTCATG
CTGGAGTTCT
TCGCCCACCC
CAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGTATACCGT
CGACCTCTAG
CTAGAGCTTG
GCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTGAC