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pX853-U6-gRNA-CBh-SpCas9

产品货号:P1105

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pX853-U6-gRNA-CBh-SpCas9
别称: Plasmid#62885
基因长:
质粒宿主: 哺乳细胞
质粒用途: 基因编辑
片段类型: CRISPR
片段物种:
原核抗性: Amp
筛选标记:
荧光标记:
启动子:
复制子:
拷贝数:
感受态: Stbl3
温度: 37度
背景载体:
正向引物:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

GAGGGCCTAT

TTCCCATGAT

TCCTTCATAT

TTGCATATAC

GATACAAGGC

TGTTAGAGAG

61

ATAATTGGAA

TTAATTTGAC

TGTAAACACA

AAGATATTAG

TACAAAATAC

GTGACGTAGA

121

AAGTAATAAT

TTCTTGGGTA

GTTTGCAGTT

TTAAAATTAT

GTTTTAAAAT

GGACTATCAT

181

ATGCTTACCG

TAACTTGAAA

GTATTTCGAT

TTCTTGGCTT

TATATATCTT

GTGGAAAGGA

241

CGAAACACCG

GGTCTTCGAG

AAGACCTGTT

TTAGAGCTAG

AAATAGCAAG

TTAAAATAAG

301

GCTAGTCCGT

TATCAACTTG

AAAAAGTGGC

ACCGAGTCGG

TGCTTTTTTG

TTTTAGAGCT

361

AGAAATAGCA

AGTTAAAATA

AGGCTAGTCC

GTTTTTAGCG

CGTGCGCCAA

TTCTGCAGAC

421

AAATGGCTCT

AGAGGTACCC

GTTACATAAC

TTACGGTAAA

TGGCCCGCCT

GGCTGACCGC

481

CCAACGACCC

CCGCCCATTG

ACGTCAATAG

TAACGCCAAT

AGGGACTTTC

CATTGACGTC

541

AATGGGTGGA

GTATTTACGG

TAAACTGCCC

ACTTGGCAGT

ACATCAAGTG

TATCATATGC

601

CAAGTACGCC

CCCTATTGAC

GTCAATGACG

GTAAATGGCC

CGCCTGGCAT

TGTGCCCAGT

661

ACATGACCTT

ATGGGACTTT

CCTACTTGGC

AGTACATCTA

CGTATTAGTC

ATCGCTATTA

721

CCATGGTCGA

GGTGAGCCCC

ACGTTCTGCT

TCACTCTCCC

CATCTCCCCC

CCCTCCCCAC

781

CCCCAATTTT

GTATTTATTT

ATTTTTTAAT

TATTTTGTGC

AGCGATGGGG

GCGGGGGGGG

841

GGGGGGGGCG

CGCGCCAGGC

GGGGCGGGGC

GGGGCGAGGG

GCGGGGCGGG

GCGAGGCGGA

901

GAGGTGCGGC

GGCAGCCAAT

CAGAGCGGCG

CGCTCCGAAA

GTTTCCTTTT

ATGGCGAGGC

961

GGCGGCGGCG

GCGGCCCTAT

AAAAAGCGAA

GCGCGCGGCG

GGCGGGAGTC

GCTGCGACGC

1021

TGCCTTCGCC

CCGTGCCCCG

CTCCGCCGCC

GCCTCGCGCC

GCCCGCCCCG

GCTCTGACTG

1081

ACCGCGTTAC

TCCCACAGGT

GAGCGGGCGG

GACGGCCCTT

CTCCTCCGGG

CTGTAATTAG

1141

CTGAGCAAGA

GGTAAGGGTT

TAAGGGATGG

TTGGTTGGTG

GGGTATTAAT

GTTTAATTAC

1201

CTGGAGCACC

TGCCTGAAAT

CACTTTTTTT

CAGGTTGGAC

CGGTGCCACC

ATGCTAGATT

1261

TAGCTAGCTT

AATCCTCGAC

AAGAAGTACA

GCATCGGCCT

GGACATCGGC

ACCAACTCTG

1321

TGGGCTGGGC

CGTGATCACC

GACGAGTACA

AGGTGCCCAG

CAAGAAATTC

AAGGTGCTGG

1381

GCAACACCGA

CCGGCACAGC

ATCAAGAAGA

ACCTGATCGG

AGCCCTGCTG

TTCGACAGCG

1441

GCGAAACAGC

CGAGGCCACC

CGGCTGAAGA

GAACCGCCAG

AAGAAGATAC

ACCAGACGGA

1501

AGAACCGGAT

CTGCTATCTG

CAAGAGATCT

TCAGCAACGA

GATGGCCAAG

GTGGACGACA

1561

GCTTCTTCCA

CAGACTGGAA

GAGTCCTTCC

TGGTGGAAGA

GGATAAGAAG

CACGAGCGGC

1621

ACCCCATCTT

CGGCAACATC

GTGGACGAGG

TGGCCTACCA

CGAGAAGTAC

CCCACCATCT

1681

ACCACCTGAG

AAAGAAACTG

GTGGACAGCA

CCGACAAGGC

CGACCTGCGG

CTGATCTATC

1741

TGGCCCTGGC

CCACATGATC

AAGTTCCGGG

GCCACTTCCT

GATCGAGGGC

GACCTGAACC

1801

CCGACAACAG

CGACGTGGAC

AAGCTGTTCA

TCCAGCTGGT

GCAGACCTAC

AACCAGCTGT

1861

TCGAGGAAAA

CCCCATCAAC

GCCAGCGGCG

TGGACGCCAA

GGCCATCCTG

TCTGCCAGAC

1921

TGAGCAAGAG

CAGACGGCTG

GAAAATCTGA

TCGCCCAGCT

GCCCGGCGAG

AAGAAGAATG

1981

GCCTGTTCGG

AAACCTGATT

GCCCTGAGCC

TGGGCCTGAC

CCCCAACTTC

AAGAGCAACT

2041

TCGACCTGGC

CGAGGATGCC

AAACTGCAGC

TGAGCAAGGA

CACCTACGAC

GACGACCTGG

2101

ACAACCTGCT

GGCCCAGATC

GGCGACCAGT

ACGCCGACCT

GTTTCTGGCC

GCCAAGAACC

2161

TGTCCGACGC

CATCCTGCTG

AGCGACATCC

TGAGAGTGAA

CACCGAGATC

ACCAAGGCCC

2221

CCCTGAGCGC

CTCTATGATC

AAGAGATACG

ACGAGCACCA

CCAGGACCTG

ACCCTGCTGA

2281

AAGCTCTCGT

GCGGCAGCAG

CTGCCTGAGA

AGTACAAAGA

GATTTTCTTC

GACCAGAGCA

2341

AGAACGGCTA

CGCCGGCTAC

ATTGACGGCG

GAGCCAGCCA

GGAAGAGTTC

TACAAGTTCA

2401

TCAAGCCCAT

CCTGGAAAAG

ATGGACGGCA

CCGAGGAACT

GCTCGTGAAG

CTGAACAGAG

2461

AGGACCTGCT

GCGGAAGCAG

CGGACCTTCG

ACAACGGCAG

CATCCCCCAC

CAGATCCACC

2521

TGGGAGAGCT

GCACGCCATT

CTGCGGCGGC

AGGAAGATTT

TTACCCATTC

CTGAAGGACA

2581

ACCGGGAAAA

GATCGAGAAG

ATCCTGACCT

TCCGCATCCC

CTACTACGTG

GGCCCTCTGG

2641

CCAGGGGAAA

CAGCAGATTC

GCCTGGATGA

CCAGAAAGAG

CGAGGAAACC

ATCACCCCCT

2701

GGAACTTCGA

GGAAGTGGTG

GACAAGGGCG

CTTCCGCCCA

GAGCTTCATC

GAGCGGATGA

2761

CCAACTTCGA

TAAGAACCTG

CCCAACGAGA

AGGTGCTGCC

CAAGCACAGC

CTGCTGTACG

2821

AGTACTTCAC

CGTGTATAAC

GAGCTGACCA

AAGTGAAATA

CGTGACCGAG

GGAATGAGAA

2881

AGCCCGCCTT

CCTGAGCGGC

GAGCAGAAAA

AGGCCATCGT

GGACCTGCTG

TTCAAGACCA

2941

ACCGGAAAGT

GACCGTGAAG

CAGCTGAAAG

AGGACTACTT

CAAGAAAATC

GAGGGTGGCG

3001

GTGGCTCGGG

TGGCGGTGGC

TCGGGTGGCG

GTGGCTCGGA

GATGTGGCAC

GAGGGCCTGG

3061

AGGAGGCCAG

CAGACTGTAC

TTCGGCGAGA

GAAACGTGAA

GGGCATGTTC

GAGGTGCTGG

3121

AGCCCCTGCA

CGCCATGATG

GAGAGAGGCC

CCCAGACCCT

GAAGGAGACC

AGCTTCAACC

3181

AGGCCTACGG

CAGAGACCTG

ATGGAGGCCC

AGGAGTGGTG

CAGAAAGTAC

ATGAAGAGCG

3241

GCAACGTGAA

GGACCTGACC

CAGGCCTGGG

ACCTGTACTA

CCACGTGTTC

AGAAGAATCA

3301

GCAAGCAGTA

AGAATTCCTA

GAGCTCGCTG

ATCAGCCTCG

ACTGTGCCTT

CTAGTTGCCA

3361

GCCATCTGTT

GTTTGCCCCT

CCCCCGTGCC

TTCCTTGACC

CTGGAAGGTG

CCACTCCCAC

3421

TGTCCTTTCC

TAATAAAATG

AGGAAATTGC

ATCGCATTGT

CTGAGTAGGT

GTCATTCTAT

3481

TCTGGGGGGT

GGGGTGGGGC

AGGACAGCAA

GGGGGAGGAT

TGGGAAGAGA

ATAGCAGGCA

3541

TGCTGGGGAG

CGGCCGCAGG

AACCCCTAGT

GATGGAGTTG

GCCACTCCCT

CTCTGCGCGC

3601

TCGCTCGCTC

ACTGAGGCCG

GGCGACCAAA

GGTCGCCCGA

CGCCCGGGCT

TTGCCCGGGC

3661

GGCCTCAGTG

AGCGAGCGAG

CGCGCAGCTG

CCTGCAGGGG

CGCCTGATGC

GGTATTTTCT

3721

CCTTACGCAT

CTGTGCGGTA

TTTCACACCG

CATACGTCAA

AGCAACCATA

GTACGCGCCC

3781

TGTAGCGGCG

CATTAAGCGC

GGCGGGTGTG

GTGGTTACGC

GCAGCGTGAC

CGCTACACTT

3841

GCCAGCGCCC

TAGCGCCCGC

TCCTTTCGCT

TTCTTCCCTT

CCTTTCTCGC

CACGTTCGCC

3901

GGCTTTCCCC

GTCAAGCTCT

AAATCGGGGG

CTCCCTTTAG

GGTTCCGATT

TAGTGCTTTA

3961

CGGCACCTCG

ACCCCAAAAA

ACTTGATTTG

GGTGATGGTT

CACGTAGTGG

GCCATCGCCC

4021

TGATAGACGG

TTTTTCGCCC

TTTGACGTTG

GAGTCCACGT

TCTTTAATAG

TGGACTCTTG

4081

TTCCAAACTG

GAACAACACT

CAACCCTATC

TCGGGCTATT

CTTTTGATTT

ATAAGGGATT

4141

TTGCCGATTT

CGGCCTATTG

GTTAAAAAAT

GAGCTGATTT

AACAAAAATT

TAACGCGAAT

4201

TTTAACAAAA

TATTAACGTT

TACAATTTTA

TGGTGCACTC

TCAGTACAAT

CTGCTCTGAT

4261

GCCGCATAGT

TAAGCCAGCC

CCGACACCCG

CCAACACCCG

CTGACGCGCC

CTGACGGGCT

4321

TGTCTGCTCC

CGGCATCCGC

TTACAGACAA

GCTGTGACCG

TCTCCGGGAG

CTGCATGTGT

4381

CAGAGGTTTT

CACCGTCATC

ACCGAAACGC

GCGAGACGAA

AGGGCCTCGT

GATACGCCTA

4441

TTTTTATAGG

TTAATGTCAT

GATAATAATG

GTTTCTTAGA

CGTCAGGTGG

CACTTTTCGG

4501

GGAAATGTGC

GCGGAACCCC

TATTTGTTTA

TTTTTCTAAA

TACATTCAAA

TATGTATCCG

4561

CTCATGAGAC

AATAACCCTG

ATAAATGCTT

CAATAATATT

GAAAAAGGAA

GAGTATGAGT

4621

ATTCAACATT

TCCGTGTCGC

CCTTATTCCC

TTTTTTGCGG

CATTTTGCCT

TCCTGTTTTT

4681

GCTCACCCAG

AAACGCTGGT

GAAAGTAAAA

GATGCTGAAG

ATCAGTTGGG

TGCACGAGTG

4741

GGTTACATCG

AACTGGATCT

CAACAGCGGT

AAGATCCTTG

AGAGTTTTCG

CCCCGAAGAA

4801

CGTTTTCCAA

TGATGAGCAC

TTTTAAAGTT

CTGCTATGTG

GCGCGGTATT

ATCCCGTATT

4861

GACGCCGGGC

AAGAGCAACT

CGGTCGCCGC

ATACACTATT

CTCAGAATGA

CTTGGTTGAG

4921

TACTCACCAG

TCACAGAAAA

GCATCTTACG

GATGGCATGA

CAGTAAGAGA

ATTATGCAGT

4981

GCTGCCATAA

CCATGAGTGA

TAACACTGCG

GCCAACTTAC

TTCTGACAAC

GATCGGAGGA

5041

CCGAAGGAGC

TAACCGCTTT

TTTGCACAAC

ATGGGGGATC

ATGTAACTCG

CCTTGATCGT

5101

TGGGAACCGG

AGCTGAATGA

AGCCATACCA

AACGACGAGC

GTGACACCAC

GATGCCTGTA

5161

GCAATGGCAA

CAACGTTGCG

CAAACTATTA

ACTGGCGAAC

TACTTACTCT

AGCTTCCCGG

5221

CAACAATTAA

TAGACTGGAT

GGAGGCGGAT

AAAGTTGCAG

GACCACTTCT

GCGCTCGGCC

5281

CTTCCGGCTG

GCTGGTTTAT

TGCTGATAAA

TCTGGAGCCG

GTGAGCGTGG

AAGCCGCGGT

5341

ATCATTGCAG

CACTGGGGCC

AGATGGTAAG

CCCTCCCGTA

TCGTAGTTAT

CTACACGACG

5401

GGGAGTCAGG

CAACTATGGA

TGAACGAAAT

AGACAGATCG

CTGAGATAGG

TGCCTCACTG

5461

ATTAAGCATT

GGTAACTGTC

AGACCAAGTT

TACTCATATA

TACTTTAGAT

TGATTTAAAA

5521

CTTCATTTTT

AATTTAAAAG

GATCTAGGTG

AAGATCCTTT

TTGATAATCT

CATGACCAAA

5581

ATCCCTTAAC

GTGAGTTTTC

GTTCCACTGA

GCGTCAGACC

CCGTAGAAAA

GATCAAAGGA

5641

TCTTCTTGAG

ATCCTTTTTT

TCTGCGCGTA

ATCTGCTGCT

TGCAAACAAA

AAAACCACCG

5701

CTACCAGCGG

TGGTTTGTTT

GCCGGATCAA

GAGCTACCAA

CTCTTTTTCC

GAAGGTAACT

5761

GGCTTCAGCA

GAGCGCAGAT

ACCAAATACT

GTCCTTCTAG

TGTAGCCGTA

GTTAGGCCAC

5821

CACTTCAAGA

ACTCTGTAGC

ACCGCCTACA

TACCTCGCTC

TGCTAATCCT

GTTACCAGTG

5881

GCTGCTGCCA

GTGGCGATAA

GTCGTGTCTT

ACCGGGTTGG

ACTCAAGACG

ATAGTTACCG

5941

GATAAGGCGC

AGCGGTCGGG

CTGAACGGGG

GGTTCGTGCA

CACAGCCCAG

CTTGGAGCGA

6001

ACGACCTACA

CCGAACTGAG

ATACCTACAG

CGTGAGCTAT

GAGAAAGCGC

CACGCTTCCC

6061

GAAGGGAGAA

AGGCGGACAG

GTATCCGGTA

AGCGGCAGGG

TCGGAACAGG

AGAGCGCACG

6121

AGGGAGCTTC

CAGGGGGAAA

CGCCTGGTAT

CTTTATAGTC

CTGTCGGGTT

TCGCCACCTC

6181

TGACTTGAGC

GTCGATTTTT

GTGATGCTCG

TCAGGGGGGC

GGAGCCTATG

GAAAAACGCC

6241

AGCAACGCGG

CCTTTTTACG

GTTCCTGGCC

TTTTGCTGGC

CTTTTGCTCA

CATGT

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