名称: | pB-CAGGS-dCas9-KRAB-MeCP2 |
---|---|
别称: | Plasmid#110824 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | Blast |
荧光标记: | |
启动子: | CAG |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 30度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACTCTTCCTT
TTTCAATATT
ATTGAAGCAT
TTATCAGGGT
TATTGTCTCA
TGAGCGGATA
CATATTTGAA
TGTATTTAGA
AAAATAAACA
AATAGGGGTT
CCGCGCACAT
TTCCCCGAAA
AGTGCCACCT
AAATTGTAAG
CGTTAATATT
TTGTTAAAAT
TCGCGTTAAA
TTTTTGTTAA
ATCAGCTCAT
TTTTTAACCA
ATAGGCCGAA
ATCGGCAAAA
TCCCTTATAA
ATCAAAAGAA
TAGACCGAGA
TAGGGTTGAG
TGTTGTTCCA
GTTTGGAACA
AGAGTCCACT
ATTAAAGAAC
GTGGACTCCA
ACGTCAAAGG
GCGAAAAACC
GTCTATCAGG
GCGATGGCCC
ACTACGTGAA
CCATCACCCT
AATCAAGTTT
TTTGGGGTCG
AGGTGCCGTA
AAGCACTAAA
TCGGAACCCT
AAAGGGAGCC
CCCGATTTAG
AGCTTGACGG
GGAAAGCCGG
CGAACGTGGC
GAGAAAGGAA
GGGAAGAAAG
CGAAAGGAGC
GGGCGCTAGG
GCGCTGGCAA
GTGTAGCGGT
CACGCTGCGC
GTAACCACCA
CACCCGCCGC
GCTTAATGCG
CCGCTACAGG
GCGCGTCCCA
TTCGCCATTC
AGGCTGCGCA
ACTGTTGGGA
AGGGCGATCG
GTGCGGGCCT
CTTCGCTATT
ACGCCAGCTG
GCGAAAGGGG
GATGTGCTGC
AAGGCGATTA
AGTTGGGTAA
CGCCAGGGTT
TTCCCAGTCA
CGACGTTGTA
AAACGACGGC
CAGTGAGCGC
GCCTCGTTCA
TTCACGTTTT
TGAACCCGTG
GAGGACGGGC
AGACTCGCGG
TGCAAATGTG
TTTTACAGCG
TGATGGAGCA
GATGAAGATG
CTCGACACGC
TGCAGAACAC
GCAGCTAGAT
TAACCCTAGA
AAGATAATCA
TATTGTGACG
TACGTTAAAG
ATAATCATGT
GTAAAATTGA
CGCATGTGTT
TTATCGGTCT
GTATATCGAG
GTTTATTTAT
TAATTTGAAT
AGATATTAAG
TTTTATTATA
TTTACACTTA
CATACTAATA
ATAAATTCAA
CAAACAATTT
ATTTATGTTT
ATTTATTTAT
TAAAAAAAAC
AAAAACTCAA
AATTTCTTCT
ATAAAGTAAC
AAAACTTTTA
TGAGGGACAG
CCCCCCCCCA
AAGCCCCCAG
GGATGTAATT
ACGTCCCTCC
CCCGCTAGGG
GGCAGCAGCG
AGCCGCCCGG
GGCTCCGCTC
CGGTCCGGCG
CTCCCCCCGC
ATCCCCGAGC
CGGCAGCGTG
CGGGGACAGC
CCGGGCACGG
GGAAGGTGGC
ACGGGATCGC
TTTCCTCTGA
ACGCTTCTCG
CTGCTCTTTG
AGCCTGCAGA
CACCTGGGGG
GATACGGGGA
AAAGGCCTCC
ACGGCCGACA
TTGATTATTG
ACTAGTTATT
AATAGTAATC
AATTACGGGG
TCATTAGTTC
ATAGCCCATA
TATGGAGTTC
CGCGTTACAT
AACTTACGGT
AAATGGCCCG
CCTGGCTGAC
CGCCCAACGA
CCCCCGCCCA
TTGACGTCAA
TAATGACGTA
TGTTCCCATA
GTAACGCCAA
TAGGGACTTT
CCATTGACGT
CAATGGGTGG
AGTATTTACG
GTAAACTGCC
CACTTGGCAG
TACATCAAGT
GTATCATATG
CCAAGTACGC
CCCCTATTGA
CGTCAATGAC
GGTAAATGGC
CCGCCTGGCA
TTATGCCCAG
TACATGACCT
TATGGGACTT
TCCTACTTGG
CAGTACATCT
ACGTATTAGT
CATCGCTATT
ACCATGGTCG
AGGTGAGCCC
CACGTTCTGC
TTCACTCTCC
CCATCTCCCC
CCCCTCCCCA
CCCCCAATTT
TGTATTTATT
TATTTTTTAA
TTATTTTGTG
CAGCGATGGG
GGCGGGGGGG
GGGGGGGGGC
GCGCGCCGGG
GGGGGGGGGG
GGGGGSGGGG
GGSGGGGSGG
GGSGRGGCGG
AGAGGKGCGG
CGGCAGCCAA
TCAGAGCGGC
GCGCTCCGAA
AGTTTCCTTT
TATGGCGAGG
CGGCGGCGGC
GGCGGCCCTA
TAAAAAGCGA
AGCGCGCGGC
GGGCGGGAGT
CGCTGCGCGC
TGCCTTCGCC
CCGTGCCCCG
CTCCGCCGCC
GCCTCGCGCC
GCCCGCCCCG
GCTCTGACTG
ACCGCGTTAC
TCCCACAGGT
GAGCGGGCGG
GACGGCCCTT
CTCCTCCGGG
CTGTAATTAG
CGCTTGGTTT
AATGACGGCT
TGTTTCTTTT
CTGTGGCTGC
GTGAAAGCCT
TGAGGGGCTC
CGGGAGGGCC
CTTTGTGCGG
GGGGAGCGGC
TCGGGGGGTG
CGTGCGTGTG
TGTGTGCGTG
GGGAGCGCCG
CGTGCGGCTC
CGCGCTGCCC
GGCGGCTGTG
AGCGCTGCGG
GCGCGGCGCG
GGGCTTTTTG
CGCTCCGCAG
TGTGCGCGAG
GGGAGCGCGG
CCGGGGGCGG
TGCCCCGCGG
TGCGGGGGGG
GCTGCGAGGG
GAACAAAGGC
TGCGTGCGGG
GTGTGTGCGT
GGGGGGGTGA
GCAGGGGGTG
TGGGCGCGTC
GGTCGGGCTG
CAACCCCCCC
TGCACCCCCC
TCCCCGAGTT
GCTGAGCACG
GCCCGGCTTC
GGGTGCGGGG
CTCCGTACGG
GGCGTGGCGC
GGGGCTCGCC
GTGCCGGGCG
GGGGGTGGCG
GCAGGTGGGG
GTGCCGGGCG
GGGCGGGGCC
GCCTCGGGCC
GGGGAGGGCT
CGGGGGAGGG
GCGCGGCGGC
CCCCGGAGCG
CCGGCGGCTG
TCGAGGCGCG
GCGAGCCGCA
GCCATTGCCT
TTTATGGTAA
TCGTGCGAGA
GGGCGCAGGG
ACTTCCTTTG
TCCCAAATCT
GTGCGGAGCC
GAAATCTGGG
AGGCGCCGCC
GCACCCCCTC
TAGCGGGCGC
GGGGCGAAGC
GGTGCGGCGC
CGGCAGGAAG
GAAATGGGCG
GGGAGGGCCT
TCGTGCGTCG
CCGCGCCGCC
GTCCCCTTCT
CCCTCTCCAG
CCTCGGGGCT
GTCCGCGGGG
GGACGGCTGC
CTTCGGGGGG
GACGGGGCAG
GGCGGGGTTC
GGCTTCTGGC
GTGTGACCGG
CGGCTCTAGA
GCCTCTGCTA
ACCATGTTCA
TGCCTTCTTC
TTTTTCCTAC
AGCTCCTGGG
CAACGTGCTG
GTTATTGTGC
TGTCTCATCA
TTTTGGCAAA
GAATCTAGAT
ACCCTCGTAA
AGGTCTAGAG
CTAGCGGATC
GAACCCTTGC
CACCATGGAC
AAGAAGTACT
CCATTGGGCT
CGCTATCGGC
ACAAACAGCG
TCGGCTGGGC
CGTCATTACG
GACGAGTACA
AGGTGCCGAG
CAAAAAATTC
AAAGTTCTGG
GCAATACCGA
TCGCCACAGC
ATAAAGAAGA
ACCTCATTGG
CGCCCTCCTG
TTCGACTCCG
GGGAGACGGC
CGAAGCCACG
CGGCTCAAAA
GAACAGCACG
GCGCAGATAT
ACCCGCAGAA
AGAATCGGAT
CTGCTACCTG
CAGGAGATCT
TTAGTAATGA
GATGGCTAAG
GTGGATGACT
CTTTCTTCCA
TAGGCTGGAG
GAGTCCTTTT
TGGTGGAGGA
GGATAAAAAG
CACGAGCGCC
ACCCAATCTT
TGGCAATATC
GTGGACGAGG
TGGCGTACCA
TGAAAAGTAC
CCAACCATAT
ATCATCTGAG
GAAGAAGCTT
GTAGACAGTA
CTGATAAGGC
TGACTTGCGG
TTGATCTATC
TCGCGCTGGC
GCATATGATC
AAATTTCGGG
GACACTTCCT
CATCGAGGGG
GACCTGAACC
CAGACAACAG
CGATGTCGAC
AAACTCTTTA
TCCAACTGGT
TCAGACTTAC
AATCAGCTTT
TCGAAGAGAA
CCCGATCAAC
GCATCCGGAG
TTGACGCCAA
AGCAATCCTG
AGCGCTAGGC
TGTCCAAATC
CCGGCGGCTC
GAAAACCTCA
TCGCACAGCT
CCCTGGGGAG
AAGAAGAACG
GCCTGTTTGG
TAATCTTATC
GCCCTGTCAC
TCGGGCTGAC
CCCCAACTTT
AAATCTAACT
TCGACCTGGC
CGAAGATGCC
AAGCTTCAAC
TGAGCAAAGA
CACCTACGAT
GATGATCTCG
ACAATCTGCT
GGCCCAGATC
GGCGACCAGT
ACGCAGACCT
TTTTTTGGCG
GCAAAGAACC
TGTCAGACGC
CATTCTGCTG
AGTGATATTC
TGCGAGTGAA
CACGGAGATC
ACCAAAGCTC
CGCTGAGCGC
TAGTATGATC
AAGCGCTATG
ATGAGCACCA
CCAAGACTTG
ACTTTGCTGA
AGGCCCTTGT
CAGACAGCAA
CTGCCTGAGA
AGTACAAGGA
AATTTTCTTC
GATCAGTCTA
AAAATGGCTA
CGCCGGATAC
ATTGACGGCG
GAGCAAGCCA
GGAGGAATTT
TACAAATTTA
TTAAGCCCAT
CTTGGAAAAA
ATGGACGGCA
CCGAGGAGCT
GCTGGTAAAG
CTTAACAGAG
AAGATCTGTT
GCGCAAACAG
CGCACTTTCG
ACAATGGAAG
CATCCCCCAC
CAGATTCACC
TGGGCGAACT
GCACGCTATC
CTCAGGCGGC
AAGAGGATTT
CTACCCCTTT
TTGAAAGATA
ACAGGGAAAA
GATTGAGAAA
ATCCTCACAT
TTCGGATACC
CTACTATGTA
GGCCCCCTCG
CCCGGGGAAA
TTCCAGATTC
GCGTGGATGA
CTCGCAAATC
AGAAGAGACC
ATCACTCCCT
GGAACTTCGA
GGAAGTCGTG
GATAAGGGGG
CCTCTGCCCA
GTCCTTCATC
GAAAGGATGA
CTAACTTTGA
TAAAAATCTG
CCTAACGAAA
AGGTGCTTCC
TAAACACTCT
CTGCTGTACG
AGTACTTCAC
AGTTTATAAC
GAGCTCACCA
AGGTCAAATA
CGTCACAGAA
GGGATGAGAA
AGCCAGCATT
CCTGTCTGGA
GAGCAGAAGA
AAGCTATCGT
GGACCTCCTC
TTCAAGACGA
ACCGGAAAGT
TACCGTGAAA
CAGCTCAAAG
AAGACTATTT
CAAAAAGATT
GAATGTTTCG
ACTCTGTTGA
AATCAGCGGA
GTGGAGGATC
GCTTCAACGC
ATCCCTGGGA
ACGTATCACG
ATCTCCTGAA
AATCATTAAA
GACAAGGACT
TCCTGGACAA
TGAGGAGAAC
GAGGACATTC
TTGAGGACAT
TGTCCTCACC
CTTACGTTGT
TTGAAGATAG
GGAGATGATT
GAAGAACGCT
TGAAAACTTA
CGCTCATCTC
TTCGACGACA
AAGTCATGAA
ACAGCTCAAG
AGGCGCCGAT
ATACAGGATG
GGGGCGGCTG
TCAAGAAAAC
TGATCAATGG
GATCCGAGAC
AAGCAGAGTG
GAAAGACAAT
CCTGGATTTT
CTTAAGTCCG
ATGGATTTGC
CAACCGGAAC
TTCATGCAGT
TGATCCATGA
TGACTCTCTC
ACCTTTAAGG
AGGACATCCA
GAAAGCACAA
GTTTCTGGCC
AGGGGGACAG
TCTTCACGAG
CACATCGCTA
ATCTTGCAGG
TAGCCCAGCT
ATCAAAAAGG
GAATACTGCA
GACCGTTAAG
GTCGTGGATG
AACTCGTCAA
AGTAATGGGA
AGGCATAAGC
CCGAGAATAT
CGTTATCGAG
ATGGCCCGAG
AGAACCAAAC
TACCCAGAAG
GGACAGAAGA
ACAGTAGGGA
AAGGATGAAG
AGGATTGAAG
AGGGTATAAA
AGAACTGGGG
TCCCAAATCC
TTAAGGAACA
CCCAGTTGAA
AACACCCAGC
TTCAGAATGA
GAAGCTCTAC
CTGTACTACC
TGCAGAACGG
CAGGGACATG
TACGTGGATC
AGGAACTGGA
CATCAATCGG
CTCTCCGACT
ACGACGTGGC
TGCTATCGTG
CCCCAGTCTT
TTCTCAAAGA
TGATTCTATT
GATAATAAAG
TGTTGACAAG
ATCCGATAAA
GCTAGAGGGA
AGAGTGATAA
CGTCCCCTCA
GAAGAAGTTG
TCAAGAAAAT
GAAAAATTAT
TGGCGGCAGC
TGCTGAACGC
CAAACTGATC
ACACAACGGA
AGTTCGATAA
TCTGACTAAG
GCTGAACGAG
GTGGCCTGTC
TGAGTTGGAT
AAAGCCGGCT
TCATCAAAAG
GCAGCTTGTT
GAGACACGCC
AGATCACCAA
GCACGTGGCC
CAAATTCTCG
ATTCACGCAT
GAACACCAAG
TACGATGAAA
ATGACAAACT
GATTCGAGAG
GTGAAAGTTA
TTACTCTGAA
GTCTAAGCTG
GTCTCAGATT
TCAGAAAGGA
CTTTCAGTTT
TATAAGGTGA
GAGAGATCAA
CAATTACCAC
CATGCGCATG
ATGCCTACCT
GAATGCAGTG
GTAGGCACTG
CACTTATCAA
AAAATATCCC
AAGCTTGAAT
CTGAATTTGT
TTACGGAGAC
TATAAAGTGT
ACGATGTTAG
GAAAATGATC
GCAAAGTCTG
AGCAGGAAAT
AGGCAAGGCC
ACCGCTAAGT
ACTTCTTTTA
CAGCAATATT
ATGAATTTTT
TCAAGACCGA
GATTACACTG
GCCAATGGAG
AGATTCGGAA
GCGACCACTT
ATCGAAACAA
ACGGAGAAAC
AGGAGAAATC
GTGTGGGACA
AGGGTAGGGA
TTTCGCGACA
GTCCGGAAGG
TCCTGTCCAT
GCCGCAGGTG
AACATCGTTA
AAAAGACCGA
AGTACAGACC
GGAGGCTTCT
CCAAGGAAAG
TATCCTCCCG
AAAAGGAACA
GCGACAAGCT
GATCGCACGC
AAAAAAGATT
GGGACCCCAA
GAAATACGGC
GGATTCGATT
CTCCTACAGT
CGCTTACAGT
GTACTGGTTG
TGGCCAAAGT
GGAGAAAGGG
AAGTCTAAAA
AACTCAAAAG
CGTCAAGGAA
CTGCTGGGCA
TCACAATCAT
GGAGCGATCA
AGCTTCGAAA
AAAACCCCAT
CGACTTTCTC
GAGGCGAAAG
GATATAAAGA
GGTCAAAAAA
GACCTCATCA
TTAAGCTTCC
CAAGTACTCT
CTCTTTGAGC
TTGAAAACGG
CCGGAAACGA
ATGCTCGCTA
GTGCGGGCGA
GCTGCAGAAA
GGTAACGAGC
TGGCACTGCC
CTCTAAATAC
GTTAATTTCT
TGTATCTGGC
CAGCCACTAT
GAAAAGCTCA
AAGGGTCTCC
CGAAGATAAT
GAGCAGAAGC
AGCTGTTCGT
GGAACAACAC
AAACACTACC
TTGATGAGAT
CATCGAGCAA
ATAAGCGAAT
TCTCCAAAAG
AGTGATCCTC
GCCGACGCTA
ACCTCGATAA
GGTGCTTTCT
GCTTACAATA
AGCACAGGGA
TAAGCCCATC
AGGGAGCAGG
CAGAAAACAT
TATCCACTTG
TTTACTCTGA
CCAACTTGGG
CGCGCCTGCA
GCCTTCAAGT
ACTTCGACAC
CACCATAGAC
AGAAAGCGGT
ACACCTCTAC
AAAGGAGGTC
CTGGACGCCA
CACTGATTCA
TCAGTCAATT
ACGGGGCTCT
ATGAAACAAG
AATCGACCTC
TCTCAGCTCG
GTGGAGACAG
CAGGGCTGAC
CCCAAGAAGA
AGAGGAAGGT
GAGTGGTGGA
GGAAGTGGCG
GGTCAGGGTC
GATGGACGCG
AAATCACTTA
CGGCATGGTC
GAGAACACTG
GTTACGTTCA
AGGACGTGTT
TGTGGACTTT
ACACGTGAGG
AGTGGAAATT
GCTGGATACT
GCGCAACAAA
TTGTGTATCG
AAATGTCATG
CTTGAGAATT
ACAAGAACCT
CGTCAGTCTC
GGATACCAGT
TGACGAAACC
GGATGTGATC
CTTAGGCTCG
AAAAGGGGGA
AGAACCTTGG
CTGGTATCGG
GAGGTGGTTC
GGGTGGCTCT
GGATCAAGCC
CAAAGAAGAA
ACGGAAGGTG
GAAGCCTCAG
TGCAGGTGAA
AAGGGTGCTG
GAAAAATCCC
CCGGCAAACT
CCTCGTGAAG
ATGCCCTTCC
AGGCTTCCCC
TGGCGGAAAA
GGTGAAGGGG
GTGGCGCAAC
CACATCTGCC
CAGGTCATGG
TCATCAAGCG
ACCTGGAAGG
AAAAGAAAGG
CCGAGGCTGA
CCCTCAGGCC
ATTCCAAAGA
AACGGGGACG
CAAGCCAGGG
TCCGTGGTCG
CAGCTGCAGC
AGCTGAGGCT
AAGAAAAAGG
CAGTGAAGGA
AAGCTCCATC
CGCAGTGTGC
AGGAGACTGT
CCTGCCCATC
AAGAAGAGGA
AGACTAGGGA
GACCGTGTCC
ATCGAGGTCA
AAGAAGTGGT
CAAGCCCCTG
CTCGTGTCCA
CCCTGGGCGA
AAAATCTGGA
AAGGGGCTCA
AAACATGCAA
GTCACCTGGA
CGGAAAAGCA
AGGAGTCTAG
TCCAAAGGGG
CGCTCAAGCT
CCGCTTCTAG
TCCCCCTAAA
AAGGAACACC
ATCACCATCA
CCATCACGCC
GAGTCTCCTA
AGGCTCCTAT
GCCACTGCTC
CCACCACCTC
CACCACCTGA
GCCACAGTCA
AGCGAAGACC
CCATCAGCCC
ACCCGAGCCT
CAGGATCTGT
CCTCTAGTAT
TTGCAAAGAG
GAAAAGATGC
CCAGAGCAGG
CAGCCTGGAG
AGTGATGGCT
GTCCAAAAGA
ACCCGCCAAG
ACCCAGCCTA
TGGTGGCAGC
CGCTGCAACT
ACCACCACAA
CCACAACTAC
CACAGTGGCC
GAAAAATACA
AGCATCGCGG
CGAGGGCGAA
CGAAAGGACA
TTGTGTCAAG
CTCCATGCCC
AGACCTAACC
GGGAGGAACC
AGTCGATAGT
AGGACACCCG
TGACTGAGAG
AGTCTCATAG
AAGAATTCGA
TCCCTACCGG
TTAGTAATGA
GTTTAAACGG
GGGAGGCTAA
CTGAAACACG
GAAGGAGACA
ATACCGGAAG
GAACCCGCGC
TATGACGGCA
ATAAAAAGAC
AGAATAAAAC
GCACGGGTGT
TGGGTCGTTT
GTTCATAAAC
GCGGGGTTCG
GTCCCAGGGC
TGGCACTCTG
TCGATACCCC
ACCGAGACCC
CATTGGGGCC
AATACGCCCG
CGTTTCTTCC
TTTTCCCCAC
CCCACCCCCC
AAGTTCGGGT
GAAGGCCCAG
GGCTCGCAGC
CAACGTCGGG
GCGGCAGGCC
CTGCCATAGC
AGATCTGCGA
AATCGGATCC
GCGGCCGCAC
TGATTCTAGC
TGCAGAGGCT
CCGGTGCCCG
TCAGTGGGCA
GAGCGCACAT
CGCCCACAGT
CCCCGAGAAG
TTGGGGGGAG
GGGTCGGCAA
TTGAACCGGT
GCCTAGAGAA
GGTGGCGCGG
GGTAAACTGG
GAAAGTGATG
TCGTGTACTG
GCTCCGCCTT
TTTCCCGAGG
GTGGGGGAGA
ACCGTATATA
AGTGCAGTAG
TCGCCGTGAA
CGTTCTTTTT
CGCAACGGGT
TTGCCGCCAG
AACACAGGAT
CCTCGAGCCA
CCATGGCCAA
GCCTTTGTCT
CAAGAAGAAT
CCACCCTCAT
TGAAAGAGCA
ACGGCTACAA
TCAACAGCAT
CCCCATCTCT
GAAGACTACA
GCGTCGCCAG
CGCAGCTCTC
TCTAGCGACG
GCCGCATCTT
CACTGGTGTC
AATGTATATC
ATTTTACTGG
GGGACCTTGT
GCAGAACTCG
TGGTGCTGGG
CACTGCTGCT
GCTGCGGCAG
CTGGCAACCT
GACTTGTATC
GTCGCGATCG
GAAATGAGAA
CAGGGGCATC
TTGAGCCCCT
GCGGACGGTG
CCGACAGGTG
CTTCTCGATC
TGCATCCTGG
GATCAAAGCC
ATAGTGAAGG
ACAGTGATGG
ACAGCCGACG
GCAGTTGGGA
TTCGTGAATT
GCTGCCCTCT
GGTTATGTGT
GGGAGGGCTA
AGTTAACTTG
TTTATTGCAG
CTTATAATGG
TTACAAATAA
AGCAATAGCA
TCACAAATTT
CACAAATAAA
GCATTTTTTT
CACTGCATTC
TAGTTGTGGT
TTGTCCAAAC
TCATCAATGT
ATCTTATCAT
GTCTGGAATT
GACTCAAATG
ATGTCAATTA
GTCTATCAGA
AGCTCATCTG
GTCTCCCTTC
CGGGGGACAA
GACATCCCTG
TTTAATATTT
AAACAGCAGT
GTTCCCAAAC
TGGGTTCTTA
TATCCCTTGC
TCTGGTCAAC
CAGGTTGCAG
GGTTTCCTGT
CCTCACAGGA
ACGAAGTCCC
TAAAGAAACA
GTGGCAGCCA
GGTTTAGCCC
CGGAATTGAC
TGGATTCCTT
TTTTAGGGCC
CATTGGTATG
GCTTTTTCCC
CGTATCCCCC
CAGGTGTCTG
CAGGCTCAAA
GAGCAGCGAG
AAGCGTTCAG
AGGAAAGCGA
TCCCGTGCCA
CCTTCCCCGT
GCCCGGGCTG
TCCCCGCACG
CTGCCGGCTC
GGGGATGCGG
GGGGAGCGCC
GGACCGGAGC
GGAGCCCCGG
GCGGCTCGCT
GCTGCCCCCT
AGCGGGGGAG
GGACGTAATT
ACATCCCTGG
GGGCTTTGGG
GGGGGGCTGT
CCCTGATATC
TATAACAAGA
AAATATATAT
ATAATAAGTT
ATCACGTAAG
TAGAACATGA
AATAACAATA
TAATTATCGT
ATGAGTTAAA
TCTTAAAAGT
CACGTAAAAG
ATAATCATGC
GTCATTTTGA
CTCACGCGGT
CGTTATAGTT
CAAAATCAGT
GACACTTACC
GCATTGACAA
GCACGCCTCA
CGGGAGCTCC
AAGCGGCGAC
TGAGATGTCC
TAAATGCACA
GCGACGGATT
CGCGCTATTT
AGAAAGAGAG
AGCAATATTT
CAAGAATGCA
TGCGTCAATT
TTACGCAGAC
TATCTTTCTA
GGGTTAATCT
AGCTGCATCA
GGATCATATC
GTCGGGTCTT
TTTTCCGGCT
CAGTCATCGC
CCAAGCTGGC
GCTATCTGGG
CATCGGGGAG
GAAGAAGCCC
GTGCCTTTTC
CCGCGAGGTT
GAAGCGGCAT
GGAAAGAGTT
TGCCGAGGAT
GACTGCTGCT
GCATTGACGT
TGAGCGAAAA
CGCACGTTTA
CCATGATGAT
TCGGGAAGGT
GTGGCCATGC
ACGCCTTTAA
CGGTGAACTG
TTCGTTCAGG
CCACCTGGGA
TACCAGTTCG
TCGCGGCTTT
TCCGGACACA
GTTCCGGATG
GTCAGCCCGA
AGCGCATCAG
CAACCCGAAC
AATACCGGCG
ACAGCCGGAA
CTGCCGTGCC
GGTGTGCAGA
TTAATGACAG
CGGTGCGGCG
CTGGGATATT
ACGTCAGCGA
GGACGGGTAT
CCTGGCTGGA
TGCCGCAGAA
ATGGACATGG
ATACCCCGTG
AGTTACCCGG
CGGGCGCGCT
TGGCGTAATC
ATGGTCATAG
CTGTTTCCTG
TGTGAAATTG
TTATCCGCTC
ACAATTCCAC
ACAACATACG
AGCCGGAAGC
ATAAAGTGTA
AAGCCTGGGG
TGCCTAATGA
GTGAGCTAAC
TCACATTAAT
TGCGTTGCGC
TCACTGCCCG
CTTTCCAGTC
GGGAAACCTG
TCGTGCCAGC
TGCATTAATG
AATCGGCCAA
CGCGCGGGGA
GAGGCGGTTT
GCGTATTGGG
CGCTCTTCCG
CTTCCTCGCT
CACTGACTCG
CTGCGCTCGG
TCGTTCGGCT
GCGGCGAGCG
GTATCAGCTC
ACTCAAAGGC
GGTAATACGG
TTATCCACAG
AATCAGGGGA
TAACGCAGGA
AAGAACATGT
GAGCAAAAGG
CCAGCAAAAG
GCCAGGAACC
GTAAAAAGGC
CGCGTTGCTG
GCGTTTTTCC
ATAGGCTCCG
CCCCCCTGAC
GAGCATCACA
AAAATCGACG
CTCAAGTCAG
AGGTGGCGAA
ACCCGACAGG
ACTATAAAGA
TACCAGGCGT
TTCCCCCTGG
AAGCTCCCTC
GTGCGCTCTC
CTGTTCCGAC
CCTGCCGCTT
ACCGGATACC
TGTCCGCCTT
TCTCCCTTCG
GGAAGCGTGG
CGCTTTCTCA
TAGCTCACGC
TGTAGGTATC
TCAGTTCGGT
GTAGGTCGTT
CGCTCCAAGC
TGGGCTGTGT
GCACGAACCC
CCCGTTCAGC
CCGACCGCTG
CGCCTTATCC
GGTAACTATC
GTCTTGAGTC
CAACCCGGTA
AGACACGACT
TATCGCCACT
GGCAGCAGCC
ACTGGTAACA
GGATTAGCAG
AGCGAGGTAT
GTAGGCGGTG
CTACAGAGTT
CTTGAAGTGG
TGGCCTAACT
ACGGCTACAC
TAGAAGAACA
GTATTTGGTA
TCTGCGCTCT
GCTGAAGCCA
GTTACCTTCG
GAAAAAGAGT
TGGTAGCTCT
TGATCCGGCA
AACAAACCAC
CGCTGGTAGC
GGTGGTTTTT
TTGTTTGCAA
GCAGCAGATT
ACGCGCAGAA
AAAAAGGATC
TCAAGAAGAT
CCTTTGATCT
TTTCTACGGG
GTCTGACGCT
CAGTGGAACG
AAAACTCACG
TTAAGGGATT
TTGGTCATGA
GATTATCAAA
AAGGATCTTC
ACCTAGATCC
TTTTAAATTA
AAAATGAAGT
TTTAAATCAA
TCTAAAGTAT
ATATGAGTAA
ACTTGGTCTG
ACAGTTACCA
ATGCTTAATC
AGTGAGGCAC
CTATCTCAGC
GATCTGTCTA
TTTCGTTCAT
CCATAGTTGC
CTGACTCCCC
GTCGTGTAGA
TAACTACGAT
ACGGGAGGGC
TTACCATCTG
GCCCCAGTGC
TGCAATGATA
CCGCGAGACC
CACGCTCACC
GGCTCCAGAT
TTATCAGCAA
TAAACCAGCC
AGCCGGAAGG
GCCGAGCGCA
GAAGTGGTCC
TGCAACTTTA
TCCGCCTCCA
TCCAGTCTAT
TAATTGTTGC
CGGGAAGCTA
GAGTAAGTAG
TTCGCCAGTT
AATAGTTTGC
GCAACGTTGT
TGCCATTGCT
ACAGGCATCG
TGGTGTCACG
CTCGTCGTTT
GGTATGGCTT
CATTCAGCTC
CGGTTCCCAA
CGATCAAGGC
GAGTTACATG
ATCCCCCATG
TTGTGCAAAA
AAGCGGTTAG
CTCCTTCGGT
CCTCCGATCG
TTGTCAGAAG
TAAGTTGGCC
GCAGTGTTAT
CACTCATGGT
TATGGCAGCA
CTGCATAATT
CTCTTACTGT
CATGCCATCC
GTAAGATGCT
TTTCTGTGAC
TGGTGAGTAC
TCAACCAAGT
CATTCTGAGA
ATAGTGTATG
CGGCGACCGA
GTTGCTCTTG
CCCGGCGTCA
ATACGGGATA
ATACCGCGCC
ACATAGCAGA
ACTTTAAAAG
TGCTCATCAT
TGGAAAACGT
TCTTCGGGGC
GAAAACTCTC
AAGGATCTTA
CCGCTGTTGA
GATCCAGTTC
GATGTAACCC
ACTCGTGCAC
CCAACTGATC
TTCAGCATCT
TTTACTTTCA
CCAGCGTTTC
TGGGTGAGCA
AAAACAGGAA
GGCAAAATGC
CGCAAAAAAG
GGAATAAGGG
CGACACGGAA
ATGTTGAATA
CTCAT