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mCherry-P4M-SidM-Pou4f3 promoter

产品货号:P10915

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: mCherry-P4M-SidM-Pou4f3 promoter
别称:
基因长:
质粒宿主: 哺乳细胞
质粒用途: 启动子检测
片段类型: Promoter
片段物种:
原核抗性: Kan
筛选标记: Neo/G418
荧光标记: 红色
启动子: Pou4f3
复制子: pUC
拷贝数:
感受态: DH5a
温度: 37度
背景载体:
正向引物:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

TAGTTATTAA

TAGTAATCAA

TTACGGGGTC

ATTAGTTCAT

AGCCCATATA

TGGAGTTCCG

61

CGTTACATAA

CTTACGGTAA

ATGGCCCGCC

TGGCTGACCG

CCCAACGACC

CCCGCCCATT

121

GACGTCAATA

ATGACGTATG

TTCCCATAGT

AACGCCAATA

GGGACTTTCC

ATTGACGTCA

181

ATGGGTGGAG

TATTTACGGT

AAACTGCCCA

CTTGGCAGTA

CATCAAGTGT

ATCATATGCC

241

AAGTACGCCC

CCTATTGACG

TCAATGACGG

TAAATGGCCC

GCCTGGCATT

ATGCCCAGTA

301

CATGACCTTA

TGGGACTTTC

CTACTTGGCA

GTACATCTAC

GTATTAGTCA

TCGCTATTAC

361

CATGTGGCTA

GGCTGGTCAA

GCTGGTTTTA

GCTGGTCATC

TCCCAGACTG

TAATTAAAAA

421

AGTAATTTAA

ATATAACTTT

ATGATGCAAA

AATAATTATT

TACTTTTACA

ATAAACATTT

481

GATTAATTTA

GCATATAAAT

ACAGTTAAAA

TCAGAATTAT

TAAACCCCAT

TTAATTTTTT

541

TTCGTTATTT

ATTTTTCCCA

TATGATGTTT

AACAGAGCAA

GGAAATTTTC

ACATTATATC

601

TGATAATATT

TTTTCTTCTG

AAGAAAGTCT

TTTTTTTAAT

TTCAGCTAGA

ATAAAAGCAG

661

TTTCTATTTT

TTTTAAACTA

TTTAGCTCAA

AATTATTAGC

TTATTTAGGC

TACATCTTTA

721

GTCTACAGAA

CAAACCATTG

TTATACAATA

ACTTGCCTTA

TTAGCCAATA

CTACCATATT

781

CTGCCTAGTT

ACCCTAATTA

ACCTAGTGAA

GCCTTTAAAC

ATCACTTTAA

ACTGTATAAA

841

ACTGTCTTGG

AAAATATCTA

GTCAAATACA

ATTTTCTGTC

ATCATGGCAA

ATATAAAATA

901

AAGCAGTTAT

TAGAGATGCG

TTATCAAAAT

ATGTTTAAAA

ATGTGTTGAA

AAAATACTCT

961

CTCTGTTAAA

CAGAAATTGG

GGGAACAATA

AACAAGGGGG

GCTAATAATT

CAGGAGGGCT

1021

ATTAATTCTG

ACTTCATTAT

ATATATATAT

TAGTACACCC

AAATTAATGT

TTGACAGGAA

1081

AAATCAAGAG

AAACATAAAT

CATATAGCAT

TTAATTGAAA

TGTCGTTGTG

TGTAATTTTA

1141

TTGTTGTTGA

AATAGCTCGT

ATTTTCTTTC

TATTCCTAAA

GATGGTGTGT

GAACCAACAC

1201

TTATTTTAAT

GGATATATAT

GTTAAATAAA

CACCCGTTTT

GTTTAAATGC

ACCAAAATTT

1261

ACCTTTTGTT

ATGCTAATTT

TCTAAGGGCG

TGAAGAGACA

GAGACCTATG

CTAATTTATA

1321

AAAAAATATC

ATTACATGCA

ATTTATTTTA

ACTGACTTCA

CGTATACATG

GACATTACTT

1381

CCACTATCAT

AAAAAAAGGA

AAAAAAGAAA

AAGAAAGAAA

GAAATGACAA

ACCTCAAGTT

1441

CCCTGCAACA

AGGTTAAATG

AAATTTAGAA

TCTTCAAGTA

ACTTTCGGCA

ACAGTTAAAA

1501

TGGCAGATTA

ATGATGAAAC

CATTCGACTT

CTGAAGCGCA

TCTAAAGATC

TCGCACACAC

1561

TACGGAAAAT

GTAAATTAAG

ATGAACCGAT

GAGATATGAA

TGGGGCATTT

TTAGGTTATG

1621

CTTGTTAAAT

ATTCATGAGC

TGAAACTTTA

TCTTGAGATG

GATAGGGACA

TGACTTTATC

1681

ATAAAAGGTT

TCATACGCTA

GATTAGTGAA

TATCATGACA

CAGTTGGCAC

AACAAGACTG

1741

TTCAGAATTT

TGCCAAGACT

TAAAAAAAAA

GGTACGCGCT

GTTAATCTGC

GTAATGAATG

1801

AGAAAATGCC

AGAGAGGTAA

TGGATGCCCG

CCACATAGCA

GATGAATAAT

TGAGCGTCTG

1861

GGGGAACAGG

AGAGGTGGAT

GGGATCGGAT

GGTGCATCTG

TGTCACTGCA

CTGCCTGGCT

1921

TCTCTTTCTA

CAGCCACCAT

CACGGTCACC

ATGGAGGTAT

AACAGGTGTT

TACTTCCAAC

1981

TACCAGAAAC

ATGTTTGCAA

TGTCATTTAC

CAAACCACTT

TGGTGGGGTG

TTCATTTAAA

2041

GTTTTTTTTT

TATATGACAT

GCTGTTTAAA

GCTTATATTT

TATATTTCAT

TCGATTGACT

2101

TGCATCAGCC

AAAGGCTAAG

AACTTGACAA

ATATATTTGA

TCGATCTATA

TTAAATTAAT

2161

ATAAAAGCAT

AATTGTGTTC

ATTTTTAATG

CCCTATAGCT

GTGACCAGGG

CAGGTGAGAG

2221

CAGCTCTGTC

ACATTCAACA

GCCAATGAAG

ATTAGGGGTC

AGCCAAGGTG

AAAGTTCGTG

2281

GAGTAGACTG

TGAGCTTCAA

CTTTCATTGC

AAGTTCAAAT

GGATTTAAAT

ACAAATACAA

2341

ATCAAAATGT

GGTAGCCTGC

TATATATTTG

TGTTATTTTA

ATAATGAACA

CAATATTCTA

2401

CACATGATAG

TTTATTTACA

TAAAAACAAA

GAATTGTCAT

AAAAAAAACA

TATATAGTAT

2461

AGCCATGCTT

GTGCATTAAA

GTGGACATGT

ATAATTCTGG

AGATACATAA

GGGTATTTTT

2521

AATTGTTATA

AGCAGCCTAC

TGTAAATAAT

TATTGTACTG

TAATTCATAA

AGTAAAACCG

2581

AAAAATTATT

TACAGAATTA

CACTTCTGTC

TACAGTTTAC

CATCCGATTT

CCTATTTTGG

2641

CATTTGAAAA

CAAAGACATT

TAATAAATTT

TGCATTGCTA

TTATCAGATT

AAAAACTGAT

2701

CTGCATTTTA

ATATGTTAAA

ATAAAAATAT

AAATTATTAC

ATTTTTTAAT

TAATTAATTA

2761

ATATCAATTT

ATTTATTTAA

TCAACGTCAA

TAAAATCATT

AGTTTTATTA

TTATGCTAAA

2821

TATGTCAATA

TTTATTTTTA

TTTTTTTAAA

TCACTTCGTT

GAAATACTGG

GTTAAATAAC

2881

CTACGAAATC

CCACGCGTGA

GTGTCAGACC

AGTTTGCCAC

TTGTATCCCG

AGTAGATGGA

2941

GGCGGGAGGG

CAGTGTGAGT

TTGGGGTGGG

GGGAGAACCC

GTGAGGCGCT

GCCAATGGAA

3001

ACTGAACACA

CACGAGGAGC

GTCGAGCCAG

AGCGCCGCGT

TTCCTCCATG

TCAGGTCCAC

3061

TCACCTGCAA

GGTGCGCAAC

GAAGGCTAGC

GCTACCGGTC

GCCACCATGG

TGAGCAAGGG

3121

CGAGGAGGAT

AACATGGCCA

TCATCAAGGA

GTTCATGCGC

TTCAAGGTGC

ACATGGAGGG

3181

CTCCGTGAAC

GGCCACGAGT

TCGAGATCGA

GGGCGAGGGC

GAGGGCCGCC

CCTACGAGGG

3241

CACCCAGACC

GCCAAGCTGA

AGGTGACCAA

GGGTGGCCCC

CTGCCCTTCG

CCTGGGACAT

3301

CCTGTCCCCT

CAGTTCATGT

ACGGCTCCAA

GGCCTACGTG

AAGCACCCCG

CCGACATCCC

3361

CGACTACTTG

AAGCTGTCCT

TCCCCGAGGG

CTTCAAGTGG

GAGCGCGTGA

TGAACTTCGA

3421

GGACGGCGGC

GTGGTGACCG

TGACCCAGGA

CTCCTCCCTG

CAGGACGGCG

AGTTCATCTA

3481

CAAGGTGAAG

CTGCGCGGCA

CCAACTTCCC

CTCCGACGGC

CCCGTAATGC

AGAAGAAGAC

3541

CATGGGCTGG

GAGGCCTCCT

CCGAGCGGAT

GTACCCCGAG

GACGGCGCCC

TGAAGGGCGA

3601

GATCAAGCAG

AGGCTGAAGC

TGAAGGACGG

CGGCCACTAC

GACGCTGAGG

TCAAGACCAC

3661

CTACAAGGCC

AAGAAGCCCG

TGCAGCTGCC

CGGCGCCTAC

AACGTCAACA

TCAAGTTGGA

3721

CATCACCTCC

CACAACGAGG

ACTACACCAT

CGTGGAACAG

TACGAACGCG

CCGAGGGCCG

3781

CCACTCCACC

GGCGGCATGG

ACGAGCTGTA

CAAGTCCGGA

CTCAGATCCA

CGGCAAGCAC

3841

GGAAAACTTT

AAAAATGTTA

AAGAAAAATA

TCAGCAAATG

CGAGGTGATG

CTTTAAAAAC

3901

AGAAATCCTG

GCTGATTTCA

AGGATAAACT

GGCTGAAGCT

ACCGATGAAC

AGAGCCTTAA

3961

GCAAATTGTT

GCTGAATTGA

AAAGTAAAGA

TGAATATAGA

ATTTTGGCTA

AGGGGCAAGG

4021

GTTAACAACC

CAGCTTTTAG

GGTTAAAGAC

AAGTTCAGTG

TCTTCATTTG

AGAAAATGGT

4081

TGAGGAAACA

AGAGAAAGTA

TTAAGTCTCA

AGAAAGACAA

ACCATTAAGA

TAAAATAAGA

4141

ATTCCCGGGT

CGACTCGACG

GTACCGCGGG

CCCGGGATCC

ACCGGATCTA

GATAACTGAT

4201

CATAATCAGC

CATACCACAT

TTGTAGAGGT

TTTACTTGCT

TTAAAAAACC

TCCCACACCT

4261

CCCCCTGAAC

CTGAAACATA

AAATGAATGC

AATTGTTGTT

GTTAACTTGT

TTATTGCAGC

4321

TTATAATGGT

TACAAATAAA

GCAATAGCAT

CACAAATTTC

ACAAATAAAG

CATTTTTTTC

4381

ACTGCATTCT

AGTTGTGGTT

TGTCCAAACT

CATCAATGTA

TCTTAACGCG

TAAATTGTAA

4441

GCGTTAATAT

TTTGTTAAAA

TTCGCGTTAA

ATTTTTGTTA

AATCAGCTCA

TTTTTTAACC

4501

AATAGGCCGA

AATCGGCAAA

ATCCCTTATA

AATCAAAAGA

ATAGACCGAG

ATAGGGTTGA

4561

GTGTTGTTCC

AGTTTGGAAC

AAGAGTCCAC

TATTAAAGAA

CGTGGACTCC

AACGTCAAAG

4621

GGCGAAAAAC

CGTCTATCAG

GGCGATGGCC

CACTACGTGA

ACCATCACCC

TAATCAAGTT

4681

TTTTGGGGTC

GAGGTGCCGT

AAAGCACTAA

ATCGGAACCC

TAAAGGGAGC

CCCCGATTTA

4741

GAGCTTGACG

GGGAAAGCCG

GCGAACGTGG

CGAGAAAGGA

AGGGAAGAAA

GCGAAAGGAG

4801

CGGGCGCTAG

GGCGCTGGCA

AGTGTAGCGG

TCACGCTGCG

CGTAACCACC

ACACCCGCCG

4861

CGCTTAATGC

GCCGCTACAG

GGCGCGTCAG

GTGGCACTTT

TCGGGGAAAT

GTGCGCGGAA

4921

CCCCTATTTG

TTTATTTTTC

TAAATACATT

CAAATATGTA

TCCGCTCATG

AGACAATAAC

4981

CCTGATAAAT

GCTTCAATAA

TATTGAAAAA

GGAAGAGTCC

TGAGGCGGAA

AGAACCAGCT

5041

GTGGAATGTG

TGTCAGTTAG

GGTGTGGAAA

GTCCCCAGGC

TCCCCAGCAG

GCAGAAGTAT

5101

GCAAAGCATG

CATCTCAATT

AGTCAGCAAC

CAGGTGTGGA

AAGTCCCCAG

GCTCCCCAGC

5161

AGGCAGAAGT

ATGCAAAGCA

TGCATCTCAA

TTAGTCAGCA

ACCATAGTCC

CGCCCCTAAC

5221

TCCGCCCATC

CCGCCCCTAA

CTCCGCCCAG

TTCCGCCCAT

TCTCCGCCCC

ATGGCTGACT

5281

AATTTTTTTT

ATTTATGCAG

AGGCCGAGGC

CGCCTCGGCC

TCTGAGCTAT

TCCAGAAGTA

5341

GTGAGGAGGC

TTTTTTGGAG

GCCTAGGCTT

TTGCAAAGAT

CGATCAAGAG

ACAGGATGAG

5401

GATCGTTTCG

CATGATTGAA

CAAGATGGAT

TGCACGCAGG

TTCTCCGGCC

GCTTGGGTGG

5461

AGAGGCTATT

CGGCTATGAC

TGGGCACAAC

AGACAATCGG

CTGCTCTGAT

GCCGCCGTGT

5521

TCCGGCTGTC

AGCGCAGGGG

CGCCCGGTTC

TTTTTGTCAA

GACCGACCTG

TCCGGTGCCC

5581

TGAATGAACT

GCAAGACGAG

GCAGCGCGGC

TATCGTGGCT

GGCCACGACG

GGCGTTCCTT

5641

GCGCAGCTGT

GCTCGACGTT

GTCACTGAAG

CGGGAAGGGA

CTGGCTGCTA

TTGGGCGAAG

5701

TGCCGGGGCA

GGATCTCCTG

TCATCTCACC

TTGCTCCTGC

CGAGAAAGTA

TCCATCATGG

5761

CTGATGCAAT

GCGGCGGCTG

CATACGCTTG

ATCCGGCTAC

CTGCCCATTC

GACCACCAAG

5821

CGAAACATCG

CATCGAGCGA

GCACGTACTC

GGATGGAAGC

CGGTCTTGTC

GATCAGGATG

5881

ATCTGGACGA

AGAGCATCAG

GGGCTCGCGC

CAGCCGAACT

GTTCGCCAGG

CTCAAGGCGA

5941

GCATGCCCGA

CGGCGAGGAT

CTCGTCGTGA

CCCATGGCGA

TGCCTGCTTG

CCGAATATCA

6001

TGGTGGAAAA

TGGCCGCTTT

TCTGGATTCA

TCGACTGTGG

CCGGCTGGGT

GTGGCGGACC

6061

GCTATCAGGA

CATAGCGTTG

GCTACCCGTG

ATATTGCTGA

AGAGCTTGGC

GGCGAATGGG

6121

CTGACCGCTT

CCTCGTGCTT

TACGGTATCG

CCGCTCCCGA

TTCGCAGCGC

ATCGCCTTCT

6181

ATCGCCTTCT

TGACGAGTTC

TTCTGAGCGG

GACTCTGGGG

TTCGAAATGA

CCGACCAAGC

6241

GACGCCCAAC

CTGCCATCAC

GAGATTTCGA

TTCCACCGCC

GCCTTCTATG

AAAGGTTGGG

6301

CTTCGGAATC

GTTTTCCGGG

ACGCCGGCTG

GATGATCCTC

CAGCGCGGGG

ATCTCATGCT

6361

GGAGTTCTTC

GCCCACCCTA

GGGGGAGGCT

AACTGAAACA

CGGAAGGAGA

CAATACCGGA

6421

AGGAACCCGC

GCTATGACGG

CAATAAAAAG

ACAGAATAAA

ACGCACGGTG

TTGGGTCGTT

6481

TGTTCATAAA

CGCGGGGTTC

GGTCCCAGGG

CTGGCACTCT

GTCGATACCC

CACCGAGACC

6541

CCATTGGGGC

CAATACGCCC

GCGTTTCTTC

CTTTTCCCCA

CCCCACCCCC

CAAGTTCGGG

6601

TGAAGGCCCA

GGGCTCGCAG

CCAACGTCGG

GGCGGCAGGC

CCTGCCATAG

CCTCAGGTTA

6661

CTCATATATA

CTTTAGATTG

ATTTAAAACT

TCATTTTTAA

TTTAAAAGGA

TCTAGGTGAA

6721

GATCCTTTTT

GATAATCTCA

TGACCAAAAT

CCCTTAACGT

GAGTTTTCGT

TCCACTGAGC

6781

GTCAGACCCC

GTAGAAAAGA

TCAAAGGATC

TTCTTGAGAT

CCTTTTTTTC

TGCGCGTAAT

6841

CTGCTGCTTG

CAAACAAAAA

AACCACCGCT

ACCAGCGGTG

GTTTGTTTGC

CGGATCAAGA

6901

GCTACCAACT

CTTTTTCCGA

AGGTAACTGG

CTTCAGCAGA

GCGCAGATAC

CAAATACTGT

6961

TCTTCTAGTG

TAGCCGTAGT

TAGGCCACCA

CTTCAAGAAC

TCTGTAGCAC

CGCCTACATA

7021

CCTCGCTCTG

CTAATCCTGT

TACCAGTGGC

TGCTGCCAGT

GGCGATAAGT

CGTGTCTTAC

7081

CGGGTTGGAC

TCAAGACGAT

AGTTACCGGA

TAAGGCGCAG

CGGTCGGGCT

GAACGGGGGG

7141

TTCGTGCACA

CAGCCCAGCT

TGGAGCGAAC

GACCTACACC

GAACTGAGAT

ACCTACAGCG

7201

TGAGCTATGA

GAAAGCGCCA

CGCTTCCCGA

AGGGAGAAAG

GCGGACAGGT

ATCCGGTAAG

7261

CGGCAGGGTC

GGAACAGGAG

AGCGCACGAG

GGAGCTTCCA

GGGGGAAACG

CCTGGTATCT

7321

TTATAGTCCT

GTCGGGTTTC

GCCACCTCTG

ACTTGAGCGT

CGATTTTTGT

GATGCTCGTC

7381

AGGGGGGCGG

AGCCTATGGA

AAAACGCCAG

CAACGCGGCC

TTTTTACGGT

TCCTGGCCTT

7441

TTGCTGGCCT

TTTGCTCACA

TGTTCTTTCC

TGCGTTATCC

CCTGATTCTG

TGGATAACCG

7501

TATTACCGCC

ATGCAT

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