名称: | pDe-CAS9-D10A |
---|---|
别称: | Plasmid#61477 |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Chl |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | UBI |
复制子: | pVS1 |
拷贝数: | |
感受态: | DB3.1 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AGTACTTTGA
TCCAACCCCT
CCGCTGCTAT
AGTGCAGTCG
GCTTCTGACG
TTCAGTGCAG
CCGTCTTCTG
AAAACGACAT
GTCGCACAAG
TCCTAAGTTA
CGCGACAGGC
TGCCGCCCTG
CCCTTTTCCT
GGCGTTTTCT
TGTCGCGTGT
TTTAGTCGCA
TAAAGTAGAA
TACTTGCGAC
TAGAACCGGA
GACATTACGC
CATGAACAAG
AGCGCCGCCG
CTGGCCTGCT
GGGCTATGCC
CGCGTCAGCA
CCGACGACCA
GGACTTGACC
AACCAACGGG
CCGAACTGCA
CGCGGCCGGC
TGCACCAAGC
TGTTTTCCGA
GAAGATCACC
GGCACCAGGC
GCGACCGCCC
GGAGCTGGCC
AGGATGCTTG
ACCACCTACG
CCCTGGCGAC
GTTGTGACAG
TGACCAGGCT
AGACCGCCTG
GCCCGCAGCA
CCCGCGACCT
ACTGGACATT
GCCGAGCGCA
TCCAGGAGGC
CGGCGCGGGC
CTGCGTAGCC
TGGCAGAGCC
GTGGGCCGAC
ACCACCACGC
CGGCCGGCCG
CATGGTGTTG
ACCGTGTTCG
CCGGCATTGC
CGAGTTCGAG
CGTTCCCTAA
TCATCGACCG
CACCCGGAGC
GGGCGCGAGG
CCGCCAAGGC
CCGAGGCGTG
AAGTTTGGCC
CCCGCCCTAC
CCTCACCCCG
GCACAGATCG
CGCACGCCCG
CGAGCTGATC
GACCAGGAAG
GCCGCACCGT
GAAAGAGGCG
GCTGCACTGC
TTGGCGTGCA
TCGCTCGACC
CTGTACCGCG
CACTTGAGCG
CAGCGAGGAA
GTGACGCCCA
CCGAGGCCAG
GCGGCGCGGT
GCCTTCCGTG
AGGACGCATT
GACCGAGGCC
GACGCCCTGG
CGGCCGCCGA
GAATGAACGC
CAAGAGGAAC
AAGCATGAAA
CCGCACCAGG
ACGGCCAGGA
CGAACCGTTT
TTCATTACCG
AAGAGATCGA
GGCGGAGATG
ATCGCGGCCG
GGTACGTGTT
CGAGCCGCCC
GCGCACGTCT
CAACCGTGCG
GCTGCATGAA
ATCCTGGCCG
GTTTGTCTGA
TGCCAAGCTG
GCGGCCTGGC
CGGCCAGCTT
GGCCGCTGAA
GAAACCGAGC
GCCGCCGTCT
AAAAAGGTGA
TGTGTATTTG
AGTAAAACAG
CTTGCGTCAT
GCGGTCGCTG
CGTATATGAT
GCGATGAGTA
AATAAACAAA
TACGCAAGGG
GAACGCATGA
AGGTTATCGC
TGTACTTAAC
CAGAAAGGCG
GGTCAGGCAA
GACGACCATC
GCAACCCATC
TAGCCCGCGC
CCTGCAACTC
GCCGGGGCCG
ATGTTCTGTT
AGTCGATTCC
GATCCCCAGG
GCAGTGCCCG
CGATTGGGCG
GCCGTGCGGG
AAGATCAACC
GCTAACCGTT
GTCGGCATCG
ACCGCCCGAC
GATTGACCGC
GACGTGAAGG
CCATCGGCCG
GCGCGACTTC
GTAGTGATCG
ACGGAGCGCC
CCAGGCGGCG
GACTTGGCTG
TGTCCGCGAT
CAAGGCAGCC
GACTTCGTGC
TGATTCCGGT
GCAGCCAAGC
CCTTACGACA
TATGGGCCAC
CGCCGACCTG
GTGGAGCTGG
TTAAGCAGCG
CATTGAGGTC
ACGGATGGAA
GGCTACAAGC
GGCCTTTGTC
GTGTCGCGGG
CGATCAAAGG
CACGCGCATC
GGCGGTGAGG
TTGCCGAGGC
GCTGGCCGGG
TACGAGCTGC
CCATTCTTGA
GTCCCGTATC
ACGCAGCGCG
TGAGCTACCC
AGGCACTGCC
GCCGCCGGCA
CAACCGTTCT
TGAATCAGAA
CCCGAGGGCG
ACGCTGCCCG
CGAGGTCCAG
GCGCTGGCCG
CTGAAATTAA
ATCAAAACTC
ATTTGAGTTA
ATGAGGTAAA
GAGAAAATGA
GCAAAAGCAC
AAACACGCTA
AGTGCCGGCC
GTCCGAGCGC
ACGCAGCAGC
AAGGCTGCAA
CGTTGGCCAG
CCTGGCAGAC
ACGCCAGCCA
TGAAGCGGGT
CAACTTTCAG
TTGCCGGCGG
AGGATCACAC
CAAGCTGAAG
ATGTACGCGG
TACGCCAAGG
CAAGACCATT
ACCGAGCTGC
TATCTGAATA
CATCGCGCAG
CTACCAGAGT
AAATGAGCAA
ATGAATAAAT
GAGTAGATGA
ATTTTAGCGG
CTAAAGGAGG
CGGCATGGAA
AATCAAGAAC
AACCAGGCAC
CGACGCCGTG
GAATGCCCCA
TGTGTGGAGG
AACGGGCGGT
TGGCCAGGCG
TAAGCGGCTG
GGTTGTCTGC
CGGCCCTGCA
ATGGCACTGG
AACCCCCAAG
CCCGAGGAAT
CGGCGTGACG
GTCGCAAACC
ATCCGGCCCG
GTACAAATCG
GCGCGGCGCT
GGGTGATGAC
CTGGTGGAGA
AGTTGAAGGC
CGCGCAGGCC
GCCCAGCGGC
AACGCATCGA
GGCAGAAGCA
CGCCCCGGTG
AATCGTGGCA
AGCGGCCGCT
GATCGAATCC
GCAAAGAATC
CCGGCAACCG
CCGGCAGCCG
GTGCGCCGTC
GATTAGGAAG
CCGCCCAAGG
GCGACGAGCA
ACCAGATTTT
TTCGTTCCGA
TGCTCTATGA
CGTGGGCACC
CGCGATAGTC
GCAGCATCAT
GGACGTGGCC
GTTTTCCGTC
TGTCGAAGCG
TGACCGACGA
GCTGGCGAGG
TGATCCGCTA
CGAGCTTCCA
GACGGGCACG
TAGAGGTTTC
CGCAGGGCCG
GCCGGCATGG
CCAGTGTGTG
GGATTACGAC
CTGGTACTGA
TGGCGGTTTC
CCATCTAACC
GAATCCATGA
ACCGATACCG
GGAAGGGAAG
GGAGACAAGC
CCGGCCGCGT
GTTCCGTCCA
CACGTTGCGG
ACGTACTCAA
GTTCTGCCGG
CGAGCCGATG
GCGGAAAGCA
GAAAGACGAC
CTGGTAGAAA
CCTGCATTCG
GTTAAACACC
ACGCACGTTG
CCATGCAGCG
TACGAAGAAG
GCCAAGAACG
GCCGCCTGGT
GACGGTATCC
GAGGGTGAAG
CCTTGATTAG
CCGCTACAAG
ATCGTAAAGA
GCGAAACCGG
GCGGCCGGAG
TACATCGAGA
TCGAGCTAGC
TGATTGGATG
TACCGCGAGA
TCACAGAAGG
CAAGAACCCG
GACGTGCTGA
CGGTTCACCC
CGATTACTTT
TTGATCGATC
CCGGCATCGG
CCGTTTTCTC
TACCGCCTGG
CACGCCGCGC
CGCAGGCAAG
GCAGAAGCCA
GATGGTTGTT
CAAGACGATC
TACGAACGCA
GTGGCAGCGC
CGGAGAGTTC
AAGAAGTTCT
GTTTCACCGT
GCGCAAGCTG
ATCGGGTCAA
ATGACCTGCC
GGAGTACGAT
TTGAAGGAGG
AGGCGGGGCA
GGCTGGCCCG
ATCCTAGTCA
TGCGCTACCG
CAACCTGATC
GAGGGCGAAG
CATCCGCCGG
TTCCTAATGT
ACGGAGCAGA
TGCTAGGGCA
AATTGCCCTA
GCAGGGGAAA
AAGGTCGAAA
AGGTCTCTTT
CCTGTGGATA
GCACGTACAT
TGGGAACCCA
AAGCCGTACA
TTGGGAACCG
GAACCCGTAC
ATTGGGAACC
CAAAGCCGTA
CATTGGGAAC
CGGTCACACA
TGTAAGTGAC
TGATATAAAA
GAGAAAAAAG
GCGATTTTTC
CGCCTAAAAC
TCTTTAAAAC
TTATTAAAAC
TCTTAAAACC
CGCCTGGCCT
GTGCATAACT
GTCTGGCCAG
CGCACAGCCG
AAGAGCTGCA
AAAAGCGCCT
ACCCTTCGGT
CGCTGCGCTC
CCTACGCCCC
GCCGCTTCGC
GTCGGCCTAT
CGCGGCCGCT
GGCCGCTCAA
AAATGGCTGG
CCTACGGCCA
GGCAATCTAC
CAGGGCGCGG
ACAAGCCGCG
CCGTCGCCAC
TCGACCGCCG
GCGCCCACAT
CAAGGCACCC
TGCCTCGCGC
GTTTCGGTGA
TGACGGTGAA
AACCTCTGAC
ACATGCAGCT
CCCGGAGACG
GTCACAGCTT
GTCTGTAAGC
GGATGCCGGG
AGCAGACAAG
CCCGTCAGGG
CGCGTCAGCG
GGTGTTGGCG
GGTGTCGGGG
CGCAGCCATG
ACCCAGTCAC
GTAGCGATAG
CGGAGTGTAT
ACTGGCTTAA
CTATGCGGCA
TCAGAGCAGA
TTGTACTGAG
AGTGCACCAT
ATGCGGTGTG
AAATACCGCA
CAGATGCGTA
AGGAGAAAAT
ACCGCATCAG
GCGCTCTTCC
GCTTCCTCGC
TCACTGACTC
GCTGCGCTCG
GTCGTTCGGC
TGCGGCGAGC
GGTATCAGCT
CACTCAAAGG
CGGTAATACG
GTTATCCACA
GAATCAGGGG
ATAACGCAGG
AAAGAACATG
TGAGCAAAAG
GCCAGCAAAA
GGCCAGGAAC
CGTAAAAAGG
CCGCGTTGCT
GGCGTTTTTC
CATAGGCTCC
GCCCCCCTGA
CGAGCATCAC
AAAAATCGAC
GCTCAAGTCA
GAGGTGGCGA
AACCCGACAG
GACTATAAAG
ATACCAGGCG
TTTCCCCCTG
GAAGCTCCCT
CGTGCGCTCT
CCTGTTCCGA
CCCTGCCGCT
TACCGGATAC
CTGTCCGCCT
TTCTCCCTTC
GGGAAGCGTG
GCGCTTTCTC
ATAGCTCACG
CTGTAGGTAT
CTCAGTTCGG
TGTAGGTCGT
TCGCTCCAAG
CTGGGCTGTG
TGCACGAACC
CCCCGTTCAG
CCCGACCGCT
GCGCCTTATC
CGGTAACTAT
CGTCTTGAGT
CCAACCCGGT
AAGACACGAC
TTATCGCCAC
TGGCAGCAGC
CACTGGTAAC
AGGATTAGCA
GAGCGAGGTA
TGTAGGCGGT
GCTACAGAGT
TCTTGAAGTG
GTGGCCTAAC
TACGGCTACA
CTAGAAGGAC
AGTATTTGGT
ATCTGCGCTC
TGCTGAAGCC
AGTTACCTTC
GGAAAAAGAG
TTGGTAGCTC
TTGATCCGGC
AAACAAACCA
CCGCTGGTAG
CGGTGGTTTT
TTTGTTTGCA
AGCAGCAGAT
TACGCGCAGA
AAAAAAGGAT
CTCAAGAAGA
TCCTTTGATC
TTTTCTACGG
GGTCTGACGC
TCAGTGGAAC
GAAAACTCAC
GTTAAGGGAT
TTTGGTCATG
CATGATATAT
CTCCCAATTT
GTGTAGGGCT
TATTATGCAC
GCTTAAAAAT
AATAAAAGCA
GACTTGACCT
GATAGTTTGG
CTGTGAGCAA
TTATGTGCTT
AGTGCATCTA
ATCGCTTGAG
TTAACGCCGG
CGAAGCGGCG
TCGGCTTGAA
CGAATTTCTA
GCTAGACATT
ATTTGCCGAC
TACCTTGGTG
ATCTCGCCTT
TCACGTAGTG
GACAAATTCT
TCCAACTGAT
CTGCGCGCGA
GGCCAAGCGA
TCTTCTTCTT
GTCCAAGATA
AGCCTGTCTA
GCTTCAAGTA
TGACGGGCTG
ATACTGGGCC
GGCAGGCGCT
CCATTGCCCA
GTCGGCAGCG
ACATCCTTCG
GCGCGATTTT
GCCGGTTACT
GCGCTGTACC
AAATGCGGGA
CAACGTAAGC
ACTACATTTC
GCTCATCGCC
AGCCCAGTCG
GGCGGCGAGT
TCCATAGCGT
TAAGGTTTCA
TTTAGCGCCT
CAAATAGATC
CTGTTCAGGA
ACCGGATCAA
AGAGTTCCTC
CGCCGCTGGA
CCTACCAAGG
CAACGCTATG
TTCTCTTGCT
TTTGTCAGCA
AGATAGCCAG
ATCAATGTCG
ATCGTGGCTG
GCTCGAAGAT
ACCTGCAAGA
ATGTCATTGC
GCTGCCATTC
TCCAAATTGC
AGTTCGCGCT
TAGCTGGATA
ACGCCACGGA
ATGATGTCGT
CGTGCACAAC
AATGGTGACT
TCTACAGCGC
GGAGAATCTC
GCTCTCTCCA
GGGGAAGCCG
AAGTTTCCAA
AAGGTCGTTG
ATCAAAGCTC
GCCGCGTTGT
TTCATCAAGC
CTTACGGTCA
CCGTAACCAG
CAAATCAATA
TCACTGTGTG
GCTTCAGGCC
GCCATCCACT
GCGGAGCCGT
ACAAATGTAC
GGCCAGCAAC
GTCGGTTCGA
GATGGCGCTC
GATGACGCCA
ACTACCTCTG
ATAGTTGAGT
CGATACTTCG
GCGATCACCG
CTTCCCCCAT
GATGTTTAAC
TTTGTTTTAG
GGCGACTGCC
CTGCTGCGTA
ACATCGTTGC
TGCTCCATAA
CATCAAACAT
CGACCCACGG
CGTAACGCGC
TTGCTGCTTG
GATGCCCGAG
GCATAGACTG
TACCCCAAAA
AAACATGTCA
TAACAAGAAG
CCATGAAAAC
CGCCACTGCG
CCGTTACCAC
CGCTGCGTTC
GGTCAAGGTT
CTGGACCAGT
TGCGTGACGG
CAGTTACGCT
ACTTGCATTA
CAGCTTACGA
ACCGAACGAG
GCTTATGTCC
ACTGGGTTCG
TGCCCGAATT
GATCACAGGC
AGCAACGCTC
TGTCATCGTT
ACAATCAACA
TGCTACCCTC
CGCGAGATCA
TCCGTGTTTC
AAACCCGGCA
GCTTAGTTGC
CGTTCTTCCG
AATAGCATCG
GTAACATGAG
CAAAGTCTGC
CGCCTTACAA
CGGCTCTCCC
GCTGACGCCG
TCCCGGACTG
ATGGGCTGCC
TGTATCGAGT
GGTGATTTTG
TGCCGAGCTG
CCGGTCGGGG
AGCTGTTGGC
TGGCTGGTGG
CAGGATATAT
TGTGGTGTAA
ACAAATTGAC
GCTTAGACAA
CTTAATAACA
CATTGCGGAC
GTTTTTAATG
TACTGAATTA
ACGCCGAATT
GCTCTAGCCA
ATACGCAAAC
CGCCTCTCCC
CGCGCGTTGG
CCGATTCATT
AATGCAGCTG
GCACGACAGG
TTTCCCGACT
GGAAAGCGGG
CAGTGAGCGC
AACGCAATTA
ATGTGAGTTA
GCTCACTCAT
TAGGCACCCC
AGGCTTTACA
CTTTATGCTT
CCGGCTCGTA
TGTTGTGTGG
AATTGTGAGC
GGATAACAAT
TTCACACAGG
AAACAGCTAT
GACATGATTA
CGAATTCAAA
AATTACGGAT
ATGAATATAG
GCATATCCGT
ATCCGAATTA
TCCGTTTGAC
AGCTAGCAAC
GATTGTACAA
TTGCTTCTTT
AAAAAAGGAA
GAAAGAAAGA
AAGAAAAGAA
TCAACATCAG
CGTTAACAAA
CGGCCCCGTT
ACGGCCCAAA
CGGTCATATA
GAGTAACGGC
GTTAAGCGTT
GAAAGACTCC
TATCGAAATA
CGTAACCGCA
AACGTGTCAT
AGTCAGATCC
CCTCTTCCTT
CACCGCCTCA
AACACAAAAA
TAATCTTCTA
CAGCCTATAT
ATACAACCCC
CCCTTCTATC
TCTCCTTTCT
CACAATTCAT
CATCTTTCTT
TCTCTACCCC
CAATTTTAAG
AAATCCTCTC
TTCTCCTCTT
CATTTTCAAG
GTAAATCTCT
CTCTCTCTCT
CTCTCTCTGT
TATTCCTTGT
TTTAATTAGG
TATGTATTAT
TGCTAGTTTG
TTAATCTGCT
TATCTTATGT
ATGCCTTATG
TGAATATCTT
TATCTTGTTC
ATCTCATCCG
TTTAGAAGCT
ATAAATTTGT
TGATTTGACT
GTGTATCTAC
ACGTGGTTAT
GTTTATATCT
AATCAGATAT
GAATTTCTTC
ATATTGTTGC
GTTTGTGTGT
ACCAATCCGA
AATCGTTGAT
TTTTTTCATT
TAATCGTGTA
GCTAATTGTA
CGTATACATA
TGGATCTACG
TATCAATTGT
TCATCTGTTT
GTGTTTGTAT
GTATACAGAT
CTGAAAACAT
CACTTCTCTC
ATCTGATTGT
GTTGTTACAT
ACATAGATAT
AGATCTGTTA
TATCATTTTT
TTTATTAATT
GTGTATATAT
ATATGTGCAT
AGATCTGGAT
TACATGATTG
TGATTATTTA
CATGATTTTG
TTATTTACGT
ATGTATATAT
GTAGATCTGG
ACTTTTTGGA
GTTGTTGACT
TGATTGTATT
TGTGTGTGTA
TATGTGTGTT
CTGATCTTGA
TATGTTATGT
ATGTGCAGCG
AATTCGGCGC
GCCATGGATA
AGAAGTACTC
TATCGGACTC
GCTATCGGAA
CTAACTCTGT
GGGATGGGCT
GTGATCACCG
ATGAGTACAA
GGTGCCATCT
AAGAAGTTCA
AGGTTCTCGG
AAACACCGAT
AGGCACTCTA
TCAAGAAAAA
CCTTATCGGT
GCTCTCCTCT
TCGATTCTGG
TGAAACTGCT
GAGGCTACCA
GACTCAAGAG
AACCGCTAGA
AGAAGGTACA
CCAGAAGAAA
GAACAGGATC
TGCTACCTCC
AAGAGATCTT
CTCTAACGAG
ATGGCTAAAG
TGGATGATTC
ATTCTTCCAC
AGGCTCGAAG
AGTCATTCCT
CGTGGAAGAA
GATAAGAAGC
ACGAGAGGCA
CCCTATCTTC
GGAAACATCG
TTGATGAGGT
GGCATACCAC
GAGAAGTACC
CTACTATCTA
CCACCTCAGA
AAGAAGCTCG
TTGATTCTAC
TGATAAGGCT
GATCTCAGGC
TCATCTACCT
CGCTCTCGCT
CACATGATCA
AGTTCAGAGG
ACACTTCCTC
ATCGAGGGTG
ATCTCAACCC
TGATAACTCT
GATGTGGATA
AGTTGTTCAT
CCAGCTCGTG
CAGACCTACA
ACCAGCTTTT
CGAAGAGAAC
CCTATCAACG
CTTCAGGTGT
GGATGCTAAG
GCTATCCTCT
CTGCTAGGCT
CTCTAAGTCA
AGAAGGCTTG
AGAACCTCAT
TGCTCAGCTC
CCTGGTGAGA
AGAAGAACGG
ACTTTTCGGA
AACTTGATCG
CTCTCTCTCT
CGGACTCACC
CCTAACTTCA
AGTCTAACTT
CGATCTCGCT
GAGGATGCAA
AGCTCCAGCT
CTCAAAGGAT
ACCTACGATG
ATGATCTCGA
TAACCTCCTC
GCTCAGATCG
GAGATCAGTA
CGCTGATTTG
TTCCTCGCTG
CTAAGAACCT
CTCTGATGCT
ATCCTCCTCA
GTGATATCCT
CAGAGTGAAC
ACCGAGATCA
CCAAGGCTCC
ACTCTCAGCT
TCTATGATCA
AGAGATACGA
TGAGCACCAC
CAGGATCTCA
CACTTCTCAA
GGCTCTTGTT
AGACAGCAGC
TCCCAGAGAA
GTACAAAGAG
ATTTTCTTCG
ATCAGTCTAA
GAACGGATAC
GCTGGTTACA
TCGATGGTGG
TGCATCTCAA
GAAGAGTTCT
ACAAGTTCAT
CAAGCCTATC
CTCGAGAAGA
TGGATGGAAC
CGAGGAACTC
CTCGTGAAGC
TCAATAGAGA
GGATCTTCTC
AGAAAGCAGA
GGACCTTCGA
TAACGGATCT
ATCCCTCATC
AGATCCACCT
CGGAGAGTTG
CACGCTATCC
TTAGAAGGCA
AGAGGATTTC
TACCCATTCC
TCAAGGATAA
CAGGGAAAAG
ATTGAGAAGA
TTCTCACCTT
CAGAATCCCT
TACTACGTGG
GACCTCTCGC
TAGAGGAAAC
TCAAGATTCG
CTTGGATGAC
CAGAAAGTCT
GAGGAAACCA
TCACCCCTTG
GAACTTCGAA
GAGGTGGTGG
ATAAGGGTGC
TAGTGCTCAG
TCTTTCATCG
AGAGGATGAC
CAACTTCGAT
AAGAACCTTC
CAAACGAGAA
GGTGCTCCCT
AAGCACTCTT
TGCTCTACGA
GTACTTCACC
GTGTACAACG
AGTTGACCAA
GGTTAAGTAC
GTGACCGAGG
GAATGAGGAA
GCCTGCTTTT
TTGTCAGGTG
AGCAAAAGAA
GGCTATCGTT
GATCTCTTGT
TCAAGACCAA
CAGAAAGGTG
ACCGTGAAGC
AGCTCAAAGA
GGATTACTTC
AAGAAAATCG
AGTGCTTCGA
TTCAGTTGAG
ATTTCTGGTG
TTGAGGATAG
GTTCAACGCA
TCTCTCGGAA
CCTACCACGA
TCTCCTCAAG
ATCATTAAGG
ATAAGGATTT
CTTGGATAAC
GAGGAAAACG
AGGATATCTT
GGAGGATATC
GTTCTTACCC
TCACCCTCTT
TGAAGATAGA
GAGATGATTG
AAGAAAGGCT
CAAGACCTAC
GCTCATCTCT
TCGATGATAA
GGTGATGAAG
CAGTTGAAGA
GAAGAAGATA
CACTGGTTGG
GGAAGGCTCT
CAAGAAAGCT
CATTAACGGA
ATCAGGGATA
AGCAGTCTGG
AAAGACAATC
CTTGATTTCC
TCAAGTCTGA
TGGATTCGCT
AACAGAAACT
TCATGCAGCT
CATCCACGAT
GATTCTCTCA
CCTTTAAAGA
GGATATCCAG
AAGGCTCAGG
TTTCAGGACA
GGGTGATAGT
CTCCATGAGC
ATATCGCTAA
CCTCGCTGGA
TCTCCTGCAA
TCAAGAAGGG
AATCCTCCAG
ACTGTGAAGG
TTGTGGATGA
GTTGGTGAAG
GTGATGGGAA
GGCATAAGCC
TGAGAACATC
GTGATCGAAA
TGGCTAGAGA
GAACCAGACC
ACTCAGAAGG
GACAGAAGAA
CTCTAGGGAA
AGGATGAAGA
GGATCGAGGA
AGGTATCAAA
GAGCTTGGAT
CTCAGATCCT
CAAAGAGCAC
CCTGTTGAGA
ACACTCAGCT
CCAGAATGAG
AAGCTCTACC
TCTACTACCT
CCAGAACGGA
AGGGATATGT
ATGTGGATCA
AGAGTTGGAT
ATCAACAGGC
TCTCTGATTA
CGATGTTGAT
CATATCGTGC
CACAGTCATT
CTTGAAGGAT
GATTCTATCG
ATAACAAGGT
GCTCACCAGG
TCTGATAAGA
ACAGGGGTAA
GAGTGATAAC
GTGCCAAGTG
AAGAGGTTGT
GAAGAAAATG
AAGAACTATT
GGAGGCAGCT
CCTCAACGCT
AAGCTCATCA
CTCAGAGAAA
GTTCGATAAC
TTGACTAAGG
CTGAGAGGGG
AGGACTCTCT
GAATTGGATA
AGGCAGGATT
CATCAAGAGG
CAGCTTGTGG
AAACCAGGCA
GATCACTAAG
CACGTTGCAC
AGATCCTCGA
TTCTAGGATG
AACACCAAGT
ACGATGAGAA
CGATAAGTTG
ATCAGGGAAG
TGAAGGTTAT
CACCCTCAAG
TCAAAGCTCG
TGTCTGATTT
CAGAAAGGAT
TTCCAATTCT
ACAAGGTGAG
GGAAATCAAC
AACTACCACC
ACGCTCACGA
TGCTTACCTT
AACGCTGTTG
TTGGAACCGC
TCTCATCAAG
AAGTATCCTA
AGCTCGAGTC
AGAGTTCGTG
TACGGTGATT
ACAAGGTGTA
CGATGTGAGG
AAGATGATCG
CTAAGTCTGA
GCAAGAGATC
GGAAAGGCTA
CCGCTAAGTA
TTTCTTCTAC
TCTAACATCA
TGAATTTCTT
CAAGACCGAG
ATTACCCTCG
CTAACGGTGA
GATCAGAAAG
AGGCCACTCA
TCGAGACAAA
CGGTGAAACA
GGTGAGATCG
TGTGGGATAA
GGGAAGGGAT
TTCGCTACCG
TTAGAAAGGT
GCTCTCTATG
CCACAGGTGA
ACATCGTTAA
GAAAACCGAG
GTGCAGACCG
GTGGATTCTC
TAAAGAGTCT
ATCCTCCCTA
AGAGGAACTC
TGATAAGCTC
ATTGCTAGGA
AGAAGGATTG
GGACCCTAAG
AAATACGGTG
GTTTCGATTC
TCCTACCGTG
GCTTACTCTG
TTCTCGTTGT
GGCTAAGGTT
GAGAAGGGAA
AGAGTAAGAA
GCTCAAGTCT
GTTAAGGAAC
TTCTCGGAAT
CACTATCATG
GAAAGGTCAT
CTTTCGAGAA
GAACCCAATC
GATTTCCTCG
AGGCTAAGGG
ATACAAAGAG
GTTAAGAAGG
ATCTCATCAT
CAAGCTCCCA
AAGTACTCAC
TCTTCGAACT
CGAGAACGGT
AGAAAGAGGA
TGCTCGCTTC
TGCTGGTGAG
CTTCAAAAGG
GAAACGAGCT
TGCTCTCCCA
TCTAAGTACG
TTAACTTTCT
TTACCTCGCT
TCTCACTACG
AGAAGTTGAA
GGGATCTCCA
GAAGATAACG
AGCAGAAGCA
ACTTTTCGTT
GAGCAGCACA
AGCACTACTT
GGATGAGATC
ATCGAGCAGA
TCTCTGAGTT
CTCTAAAAGG
GTGATCCTCG
CTGATGCAAA
CCTCGATAAG
GTGTTGTCTG
CTTACAACAA
GCACAGAGAT
AAGCCTATCA
GGGAACAGGC
AGAGAACATC
ATCCATCTCT
TCACCCTTAC
CAACCTCGGT
GCTCCTGCTG
CTTTCAAGTA
CTTCGATACA
ACCATCGATA
GGAAGAGATA
CACCTCTACC
AAAGAAGTGC
TCGATGCTAC
CCTCATCCAT
CAGTCTATCA
CTGGACTCTA
CGAGACTAGG
ATCGATCTCT
CACAGCTCGG
TGGTGATTCA
AGGGCTGATC
CTAAGAAGAA
GAGGAAGGTT
TGAGGCGCGC
CGAGCTCCAG
GCCTCCCAGC
TTTCGTCCGT
ATCATCGGTT
TCGACAACGT
TCGTCAAGTT
CAATGCATCA
GTTTCATTGC
CCACACACCA
GAATCCTACT
AAGTTTGAGT
ATTATGGCAT
TGGAAAAGCT
GTTTTCTTCT
ATCATTTGTT
CTGCTTGTAA
TTTACTGTGT
TCTTTCAGTT
TTTGTTTTCG
GACATCAAAA
TGCAAATGGA
TGGATAAGAG
TTAATAAATG
ATATGGTCCT
TTTGTTCATT
CTCAAATTAT
TATTATCTGT
TGTTTTTACT
TTAATGGGTT
GAATTTAAGT
AAGAAAGGAA
CTAACAGTGT
GATATTAAGG
TGCAATGTTA
GACATATAAA
ACAGTCTTTC
ACCTCTCTTT
GGTTATGTCT
TGAATTGGTT
TGTTTCTTCA
CTTATCTGTG
TAATCAAGTT
TACTATGAGT
CTATGATCAA
GTAATTATGC
AATCAAGTTA
AGTACAGTAT
AGGCTTGAGC
TCCCTAGGCC
TGTTATCCCT
AACAAGTTTG
TACAAAAAAG
CTGAACGAGA
AACGTAAAAT
GATATAAATA
TCAATATATT
AAATTAGATT
TTGCATAAAA
AACAGACTAC
ATAATACTGT
AAAACACAAC
ATATCCAGTC
ACTATGGCGG
CCGCATTAGG
CACCCCAGGC
TTTACACTTT
ATGCTTCCGG
CTCGTATAAT
GTGTGGATTT
TGAGTTAGGA
TCCGTCGAGA
TTTTCAGGAG
CTAAGGAAGC
TAAAATGGAG
AAAAAAATCA
CTGGATATAC
CACCGTTGAT
ATATCCCAAT
GGCATCGTAA
AGAACATTTT
GAGGCATTTC
AGTCAGTTGC
TCAATGTACC
TATAACCAGA
CCGTTCAGCT
GGATATTACG
GCCTTTTTAA
AGACCGTAAA
GAAAAATAAG
CACAAGTTTT
ATCCGGCCTT
TATTCACATT
CTTGCCCGCC
TGATGAATGC
TCATCCGGAA
TTCCGTATGG
CAATGAAAGA
CGGTGAGCTG
GTGATATGGG
ATAGTGTTCA
CCCTTGTTAC
ACCGTTTTCC
ATGAGCAAAC
TGAAACGTTT
TCATCGCTCT
GGAGTGAATA
CCACGACGAT
TTCCGGCAGT
TTCTACACAT
ATATTCGCAA
GATGTGGCGT
GTTACGGTGA
AAACCTGGCC
TATTTCCCTA
AAGGGTTTAT
TGAGAATATG
TTTTTCGTCT
CAGCCAATCC
CTGGGTGAGT
TTCACCAGTT
TTGATTTAAA
CGTGGCCAAT
ATGGACAACT
TCTTCGCCCC
CGTTTTCACC
ATGGGCAAAT
ATTATACGCA
AGGCGACAAG
GTGCTGATGC
CGCTGGCGAT
TCAGGTTCAT
CATGCCGTTT
GTGATGGCTT
CCATGTCGGC
AGAATGCTTA
ATGAATTACA
ACAGTACTGC
GATGAGTGGC
AGGCGGGGCG
TAATCTAGAG
GATCCGGCTT
ACTAAAAGCC
AGATAACAGT
ATGCGTATTT
GCGCGCTGAT
TTTTGCGGTA
TAAGAATATA
TACTGATATG
TATACCCGAA
GTATGTCAAA
AAGAGGTATG
CTATGAAGCA
GCGTATTACA
GTGACAGTTG
ACAGCGACAG
CTATCAGTTG
CTCAAGGCAT
ATATGATGTC
AATATCTCCG
GTCTGGTAAG
CACAACCATG
CAGAATGAAG
CCCGTCGTCT
GCGTGCCGAA
CGCTGGAAAG
CGGAAAATCA
GGAAGGGATG
GCTGAGGTCG
CCCGGTTTAT
TGAAATGAAC
GGCTCTTTTG
CTGACGAGAA
CAGGGGCTGG
TGAAATGCAG
TTTAAGGTTT
ACACCTATAA
AAGAGAGAGC
CGTTATCGTC
TGTTTGTGGA
TGTACAGAGT
GATATTATTG
ACACGCCCGG
GCGACGGATG
GTGATCCCCC
TGGCCAGTGC
ACGTCTGCTG
TCAGATAAAG
TCCCCCGTGA
ACTTTACCCG
GTGGTGCATA
TCGGGGATGA
AAGCTGGCGC
ATGATGACCA
CCGATATGGC
CAGTGTGCCG
GTCTCCGTTA
TCGGGGAAGA
AGTGGCTGAT
CTCAGCCACC
GCGAAAATGA
CATCAAAAAC
GCCATTAACC
TGATGTTCTG
GGGAATATAA
ATGTCAGGCT
CCCTTATACA
CAGCCAGTCT
GCAGGTCGAC
CATAGTGACT
GGATATGTTG
TGTTTTACAG
TATTATGTAG
TCTGTTTTTT
ATGCAAAATC
TAATTTAATA
TATTGATATT
TATATCATTT
TACGTTTCTC
GTTCAGCTTT
CTTGTACAAA
GTGGTTCGAT
AATTCCGATC
CAGCCTAGGC
CCGGGCCTGA
GGACGCGTCC
ATGGTTAATT
AAGACGTCCG
GACCGACTAG
TGGATCCTCT
AGAGTCGACC
TGCAGGCATG
CAAGCTTCAG
CTTGCCAACA
TGGTGGAGCA
CGACACTCTC
GTCTACTCCA
AGAATATCAA
AGATACAGTC
TCAGAAGACC
AAAGGGCTAT
TGAGACTTTT
CAACAAAGGG
TAATATCGGG
AAACCTCCTC
GGATTCCATT
GCCCAGCTAT
CTGTCACTTC
ATCAAAAGGA
CAGTAGAAAA
GGAAGGTGGC
ACCTACAAAT
GCCATCATTG
CGATAAAGGA
AAGGCTATCG
TTCAAGATGC
CTCTGCCGAC
AGTGGTCCCA
AAGATGGACC
CCCACCCACG
AGGAGCATCG
TGGAAAAAGA
AGACGTTCCA
ACCACGTCTT
CAAAGCAAGT
GGATTGATGT
GATAACATGG
TGGAGCACGA
CACTCTCGTC
TACTCCAAGA
ATATCAAAGA
TACAGTCTCA
GAAGACCAAA
GGGCTATTGA
GACTTTTCAA
CAAAGGGTAA
TATCGGGAAA
CCTCCTCGGA
TTCCATTGCC
CAGCTATCTG
TCACTTCATC
AAAAGGACAG
TAGAAAAGGA
AGGTGGCACC
TACAAATGCC
ATCATTGCGA
TAAAGGAAAG
GCTATCGTTC
AAGATGCCTC
TGCCGACAGT
GGTCCCAAAG
ATGGACCCCC
ACCCACGAGG
AGCATCGTGG
AAAAAGAAGA
CGTTCCAACC
ACGTCTTCAA
AGCAAGTGGA
TTGATGTGAT
ATCTCCACTG
ACGTAAGGGA
TGACGCACAA
TCCCACTATC
CTTCGCAAGA
CCCTTCCTCT
ATATAAGGAA
GTTCATTTCA
TTTGGAGAGG
ACACGCTGAA
ATCACCAGTC
TCTCTCTACA
AATCTATCTC
TCTCGATTCG
CAGATCTGTC
GATCGACCAT
GGGGATTGAA
CAAGATGGAT
TGCACGCAGG
TTCTCCGGCC
GCTTGGGTGG
AGAGGCTATT
CGGCTATGAC
TGGGCACAAC
AGACAATCGG
CTGCTCTGAT
GCCGCCGTGT
TCCGGCTGTC
AGCGCAGGGG
CGCCCGGTTC
TTTTTGTCAA
GACCGACCTG
TCCGGTGCCC
TGAATGAACT
CCAGGACGAG
GCAGCGCGGC
TATCGTGGCT
GGCCACGACG
GGCGTTCCTT
GCGCAGCTGT
GCTCGACGTT
GTCACTGAAG
CGGGAAGGGA
CTGGCTGCTA
TTGGGCGAAG
TGCCGGGGCA
GGATCTCCTG
TCATCTCACC
TTGCTCCTGC
CGAGAAAGTA
TCCATCATGG
CTGATGCAAT
GCGGCGGCTG
CATACGCTTG
ATCCGGCTAC
CTGCCCATTC
GACCACCAAG
CGAAACATCG
CATCGAGCGA
GCACGTACTC
GGATGGAAGC
CGGTCTTGTC
GATCAGGATG
ATCTGGACGA
AGAGCATCAG
GGGCTCGCGC
CAGCCGAACT
GTTCGCCAGG
CTCAAGGCGC
GCATGCCCGA
CGGCGAGGAT
CTCGTCGTGA
CACATGGCGA
TGCCTGCTTG
CCGAATATCA
TGGTGGAAAA
TGGCCGCTTT
TCTGGATTCA
TCGACTGTGG
CCGGCTGGGT
GTGGCGGACC
GCTATCAGGA
CATAGCGTTG
GCTACCCGTG
ATATTGCTGA
AGAGCTTGGC
GGCGAATGGG
CTGACCGCTT
CCTCGTGCTT
TACGGTATCG
CCGCTCCCGA
TTCGCAGCGC
ATCGCCTTCT
ATCGCCTTCT
TGACGAGTTC
TTCTGAGCGG
GACTCTGGGG
TTCGGATCGA
TCCTCTAGCT
AGAGTCGATC
GACATCGAGT
TTCTCCATAA
TAATGTGTGA
GTAGTTCCCA
GATAAGGGAA
TTAGGGTTCT
TATAGGGTTT
CGCTCACGTG
TTGAGCATAT
AAGAAACCCT
TAGTATGTAT
TTGTATTTGT
AAAATACTTC
TATCAATAAA
ATTTCTAATT
CCTAAAACCA
AAATCCAGTA
CTAAAATCCA
GATCACCTAA
AGTCCCTATA
GATCCCCCGA
ATTAATTCGG
CGTTAATTCA
GTACATTAAA
AACGTCCGCA
ATGTGTTATT
AAGTTGTCAA
GCTTGGCACT
GGCCGTCGTT
TTACAACGTC
GTGACTGGGA
AAACCCTGGC
GTTACCCAAC
TTAATCGCCT
TGCAGCACAT
CCCCCTTTCG
CCAGCTGGCG
TAATAGCGAA
GAGGCCCGCA
CCGATCGCCC
TTCCCAACAG
TTGCGCAGCC
TGAATGGCGA
ATGAGCTTGA
GCTTGGATCA
GATTGTCGTT
TCCCGCCTTC
AGTTTAAACT
ATCAGTGTTT
GACAGGATAT
ATTGGCGGGT
AAACCTAAGA
GAAAAGAGCG
TTTATTAGAA
TAACGGATAT
TTAAAAGGGC
GTGAAAAGGT
TTATCCGTTC
GTCCATTTGT
ATGTGCATGC
CAACCACAGG
GTTCCCCTCG
GGATCAA