名称: | pUB-Cas9 |
---|---|
别称: | Plasmid#86556 |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Kan |
筛选标记: | Hyg |
荧光标记: | |
启动子: | UBI |
复制子: | pVS1 |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TTCGTAATCA
TGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGCTA
GAGCAGCTTG
CCAACATGGT
GGAGCACGAC
ACTCTCGTCT
ACTCCAAGAA
TATCAAAGAT
ACAGTCTCAG
AAGACCAAAG
GGCTATTGAG
ACTTTTCAAC
AAAGGGTAAT
ATCGGGAAAC
CTCCTCGGAT
TCCATTGCCC
AGCTATCTGT
CACTTCATCA
AAAGGACAGT
AGAAAAGGAA
GGTGGCACCT
ACAAATGCCA
TCATTGCGAT
AAAGGAAAGG
CTATCGTTCA
AGATGCCTCT
GCCGACAGTG
GTCCCAAAGA
TGGACCCCCA
CCCACGAGGA
GCATCGTGGA
AAAAGAAGAC
GTTCCAACCA
CGTCTTCAAA
GCAAGTGGAT
TGATGTGAAC
ATGGTGGAGC
ACGACACTCT
CGTCTACTCC
AAGAATATCA
AAGATACAGT
CTCAGAAGAC
CAAAGGGCTA
TTGAGACTTT
TCAACAAAGG
GTAATATCGG
GAAACCTCCT
CGGATTCCAT
TGCCCAGCTA
TCTGTCACTT
CATCAAAAGG
ACAGTAGAAA
AGGAAGGTGG
CACCTACAAA
TGCCATCATT
GCGATAAAGG
AAAGGCTATC
GTTCAAGATG
CCTCTGCCGA
CAGTGGTCCC
AAAGATGGAC
CCCCACCCAC
GAGGAGCATC
GTGGAAAAAG
AAGACGTTCC
AACCACGTCT
TCAAAGCAAG
TGGATTGATG
TGATATCTCC
ACTGACGTAA
GGGATGACGC
ACAATCCCAC
TATCCTTCGC
AAGACCCTTC
CTCTATATAA
GGAAGTTCAT
TTCATTTGGA
GAGGACACGC
TGAAATCACC
AGTCTCTCTC
TACAAATCTA
TCTCTCTCGA
GCTTTCGCAG
ATCCGGGGGG
CAATGAGATA
TGAAAAAGCC
TGAACTCACC
GCGACGTCTG
TCGAGAAGTT
TCTGATCGAA
AAGTTCGACA
GCGTCTCCGA
CCTGATGCAG
CTCTCGGAGG
GCGAAGAATC
TCGTGCTTTC
AGCTTCGATG
TAGGAGGGCG
TGGATATGTC
CTGCGGGTAA
ATAGCTGCGC
CGATGGTTTC
TACAAAGATC
GTTATGTTTA
TCGGCACTTT
GCATCGGCCG
CGCTCCCGAT
TCCGGAAGTG
CTTGACATTG
GGGAGTTTAG
CGAGAGCCTG
ACCTATTGCA
TCTCCCGCCG
TTCACAGGGT
GTCACGTTGC
AAGACCTGCC
TGAAACCGAA
CTGCCCGCTG
TTCTACAACC
GGTCGCGGAG
GCTATGGATG
CGATCGCTGC
GGCCGATCTT
AGCCAGACGA
GCGGGTTCGG
CCCATTCGGA
CCGCAAGGAA
TCGGTCAATA
CACTACATGG
CGTGATTTCA
TATGCGCGAT
TGCTGATCCC
CATGTGTATC
ACTGGCAAAC
TGTGATGGAC
GACACCGTCA
GTGCGTCCGT
CGCGCAGGCT
CTCGATGAGC
TGATGCTTTG
GGCCGAGGAC
TGCCCCGAAG
TCCGGCACCT
CGTGCACGCG
GATTTCGGCT
CCAACAATGT
CCTGACGGAC
AATGGCCGCA
TAACAGCGGT
CATTGACTGG
AGCGAGGCGA
TGTTCGGGGA
TTCCCAATAC
GAGGTCGCCA
ACATCTTCTT
CTGGAGGCCG
TGGTTGGCTT
GTATGGAGCA
GCAGACGCGC
TACTTCGAGC
GGAGGCATCC
GGAGCTTGCA
GGATCGCCAC
GACTCCGGGC
GTATATGCTC
CGCATTGGTC
TTGACCAACT
CTATCAGAGC
TTGGTTGACG
GCAATTTCGA
TGATGCAGCT
TGGGCGCAGG
GTCGATGCGA
CGCAATCGTC
CGATCCGGAG
CCGGGACTGT
CGGGCGTACA
CAAATCGCCC
GCAGAAGCGC
GGCCGTCTGG
ACCGATGGCT
GTGTAGAAGT
ACTCGCCGAT
AGTGGAAACC
GACGCCCCAG
CACTCGTCCG
AGGGCAAAGA
AATAGAGTAG
ATGCCGACCG
GGATCTGTCG
ATCGACAAGC
TCGAGTTTCT
CCATAATAAT
GTGTGAGTAG
TTCCCAGATA
AGGGAATTAG
GGTTCCTATA
GGGTTTCGCT
CATGTGTTGA
GCATATAAGA
AACCCTTAGT
ATGTATTTGT
ATTTGTAAAA
TACTTCTATC
AATAAAATTT
CTAATTCCTA
AAACCAAAAT
CCAGTACTAA
AATCCAGATC
CCCCGAATTA
ATTCGGCGTT
AATTCAGTAC
ATTAAAAACG
TCCGCAATGT
GTTATTAAGT
TGTCTAAGCG
TCAATTTGTT
TACACCACAA
TATATCCTGC
CACCAGCCAG
CCAACAGCTC
CCCGACCGGC
AGCTCGGCAC
AAAATCACCA
CTCGATACAG
GCAGCCCATC
AGTCCGGGAC
GGCGTCAGCG
GGAGAGCCGT
TGTAAGGCGG
CAGACTTTGC
TCATGTTACC
GATGCTATTC
GGAAGAACGG
CAACTAAGCT
GCCGGGTTTG
AAACACGGAT
GATCTCGCGG
AGGGTAGCAT
GTTGATTGTA
ACGATGACAG
AGCGTTGCTG
CCTGTGATCA
CCGCGGTTTC
AAAATCGGCT
CCGTCGATAC
TATGTTATAC
GCCAACTTTG
AAAACAACTT
TGAAAAAGCT
GTTTTCTGGT
ATTTAAGGTT
TTAGAATGCA
AGGAACAGTG
AATTGGAGTT
CGTCTTGTTA
TAATTAGCTT
CTTGGGGTAT
CTTTAAATAC
TGTAGAAAAG
AGGAAGGAAA
TAATAAATGG
CTAAAATGAG
AATATCACCG
GAATTGAAAA
AACTGATCGA
AAAATACCGC
TGCGTAAAAG
ATACGGAAGG
AATGTCTCCT
GCTAAGGTAT
ATAAGCTGGT
GGGAGAAAAT
GAAAACCTAT
ATTTAAAAAT
GACGGACAGC
CGGTATAAAG
GGACCACCTA
TGATGTGGAA
CGGGAAAAGG
ACATGATGCT
ATGGCTGGAA
GGAAAGCTGC
CTGTTCCAAA
GGTCCTGCAC
TTTGAACGGC
ATGATGGCTG
GAGCAATCTG
CTCATGAGTG
AGGCCGATGG
CGTCCTTTGC
TCGGAAGAGT
ATGAAGATGA
ACAAAGCCCT
GAAAAGATTA
TCGAGCTGTA
TGCGGAGTGC
ATCAGGCTCT
TTCACTCCAT
CGACATATCG
GATTGTCCCT
ATACGAATAG
CTTAGACAGC
CGCTTAGCCG
AATTGGATTA
CTTACTGAAT
AACGATCTGG
CCGATGTGGA
TTGCGAAAAC
TGGGAAGAAG
ACACTCCATT
TAAAGATCCG
CGCGAGCTGT
ATGATTTTTT
AAAGACGGAA
AAGCCCGAAG
AGGAACTTGT
CTTTTCCCAC
GGCGACCTGG
GAGACAGCAA
CATCTTTGTG
AAAGATGGCA
AAGTAAGTGG
CTTTATTGAT
CTTGGGAGAA
GCGGCAGGGC
GGACAAGTGG
TATGACATTG
CCTTCTGCGT
CCGGTCGATC
AGGGAGGATA
TCGGGGAAGA
ACAGTATGTC
GAGCTATTTT
TTGACTTACT
GGGGATCAAG
CCTGATTGGG
AGAAAATAAA
ATATTATATT
TTACTGGATG
AATTGTTTTA
GTACCTAGAA
TGCATGACCA
AAATCCCTTA
ACGTGAGTTT
TCGTTCCACT
GAGCGTCAGA
CCCCGTAGAA
AAGATCAAAG
GATCTTCTTG
AGATCCTTTT
TTTCTGCGCG
TAATCTGCTG
CTTGCAAACA
AAAAAACCAC
CGCTACCAGC
GGTGGTTTGT
TTGCCGGATC
AAGAGCTACC
AACTCTTTTT
CCGAAGGTAA
CTGGCTTCAG
CAGAGCGCAG
ATACCAAATA
CTGTCCTTCT
AGTGTAGCCG
TAGTTAGGCC
ACCACTTCAA
GAACTCTGTA
GCACCGCCTA
CATACCTCGC
TCTGCTAATC
CTGTTACCAG
TGGCTGCTGC
CAGTGGCGAT
AAGTCGTGTC
TTACCGGGTT
GGACTCAAGA
CGATAGTTAC
CGGATAAGGC
GCAGCGGTCG
GGCTGAACGG
GGGGTTCGTG
CACACAGCCC
AGCTTGGAGC
GAACGACCTA
CACCGAACTG
AGATACCTAC
AGCGTGAGCT
ATGAGAAAGC
GCCACGCTTC
CCGAAGGGAG
AAAGGCGGAC
AGGTATCCGG
TAAGCGGCAG
GGTCGGAACA
GGAGAGCGCA
CGAGGGAGCT
TCCAGGGGGA
AACGCCTGGT
ATCTTTATAG
TCCTGTCGGG
TTTCGCCACC
TCTGACTTGA
GCGTCGATTT
TTGTGATGCT
CGTCAGGGGG
GCGGAGCCTA
TGGAAAAACG
CCAGCAACGC
GGCCTTTTTA
CGGTTCCTGG
CCTTTTGCTG
GCCTTTTGCT
CACATGTTCT
TTCCTGCGTT
ATCCCCTGAT
TCTGTGGATA
ACCGTATTAC
CGCCTTTGAG
TGAGCTGATA
CCGCTCGCCG
CAGCCGAACG
ACCGAGCGCA
GCGAGTCAGT
GAGCGAGGAA
GCGGAAGAGC
GCCTGATGCG
GTATTTTCTC
CTTACGCATC
TGTGCGGTAT
TTCACACCGC
ATATGGTGCA
CTCTCAGTAC
AATCTGCTCT
GATGCCGCAT
AGTTAAGCCA
GTATACACTC
CGCTATCGCT
ACGTGACTGG
GTCATGGCTG
CGCCCCGACA
CCCGCCAACA
CCCGCTGACG
CGCCCTGACG
GGCTTGTCTG
CTCCCGGCAT
CCGCTTACAG
ACAAGCTGTG
ACCGTCTCCG
GGAGCTGCAT
GTGTCAGAGG
TTTTCACCGT
CATCACCGAA
ACGCGCGAGG
CAGGGTGCCT
TGATGTGGGC
GCCGGCGGTC
GAGTGGCGAC
GGCGCGGCTT
GTCCGCGCCC
TGGTAGATTG
CCTGGCCGTA
GGCCAGCCAT
TTTTGAGCGG
CCAGCGGCCG
CGATAGGCCG
ACGCGAAGCG
GCGGGGCGTA
GGGAGCGCAG
CGACCGAAGG
GTAGGCGCTT
TTTGCAGCTC
TTCGGCTGTG
CGCTGGCCAG
ACAGTTATGC
ACAGGCCAGG
CGGGTTTTAA
GAGTTTTAAT
AAGTTTTAAA
GAGTTTTAGG
CGGAAAAATC
GCCTTTTTTC
TCTTTTATAT
CAGTCACTTA
CATGTGTGAC
CGGTTCCCAA
TGTACGGCTT
TGGGTTCCCA
ATGTACGGGT
TCCGGTTCCC
AATGTACGGC
TTTGGGTTCC
CAATGTACGT
GCTATCCACA
GGAAAGAGAC
CTTTTCGACC
TTTTTCCCCT
GCTAGGGCAA
TTTGCCCTAG
CATCTGCTCC
GTACATTAGG
AACCGGCGGA
TGCTTCGCCC
TCGATCAGGT
TGCGGTAGCG
CATGACTAGG
ATCGGGCCAG
CCTGCCCCGC
CTCCTCCTTC
AAATCGTACT
CCGGCAGGTC
ATTTGACCCG
ATCAGCTTGC
GCACGGTGAA
ACAGAACTTC
TTGAACTCTC
CGGCGCTGCC
ACTGCGTTCG
TAGATCGTCT
TGAACAACCA
TCTGGCTTCT
GCCTTGCCTG
CGGCGCGGCG
TGCCAGGCGG
TAGAGAAAAC
GGCCGATGCC
GGGATCGATC
AAAAAGTAAT
CGGGGTGAAC
CGTCAGCACG
TCCGGGTTCT
TGCCTTCTGT
GATCTCGCGG
TACATCCAAT
CAGCTAGCTC
GATCTCGATG
TACTCCGGCC
GCCCGGTTTC
GCTCTTTACG
ATCTTGTAGC
GGCTAATCAA
GGCTTCACCC
TCGGATACCG
TCACCAGGCG
GCCGTTCTTG
GCCTTCTTCG
TACGCTGCAT
GGCAACGTGC
GTGGTGTTTA
ACCGAATGCA
GGTTTCTACC
AGGTCGTCTT
TCTGCTTTCC
GCCATCGGCT
CGCCGGCAGA
ACTTGAGTAC
GTCCGCAACG
TGTGGACGGA
ACACGCGGCC
GGGCTTGTCT
CCCTTCCCTT
CCCGGTATCG
GTTCATGGAT
TCGGTTAGAT
GGGAAACCGC
CATCAGTACC
AGGTCGTAAT
CCCACACACT
GGCCATGCCG
GCCGGCCCTG
CGGAAACCTC
TACGTGCCCG
TCTGGAAGCT
CGTAGCGGAT
CACCTCGCCA
GCTCGTCGGT
CACGCTTCGA
CAGACGGAAA
ACGGCCACGT
CCATGATGCT
GCGACTATCG
CGGGTGCCCA
CGTCATAGAG
CATCGGAACG
AAAAAATCTG
GTTGCTCGTC
GCCCTTGGGC
GGCTTCCTAA
TCGACGGCGC
ACCGGCTGCC
GGCGGTTGCC
GGGATTCTTT
GCGGATTCGA
TCAGCGGCCG
CTTGCCACGA
TTCACCGGGG
CGTGCTTCTG
CCTCGATGCG
TTGCCGCTGG
GCGGCCTGCG
CGGCCTTCAA
CTTCTCCACC
AGGTCATCAC
CCAGCGCCGC
GCCGATTTGT
ACCGGGCCGG
ATGGTTTGCG
ACCGCTCACG
CCGATTCCTC
GGGCTTGGGG
GTTCCAGTGC
CATTGCAGGG
CCGGCAGGCA
ACCCAGCCGC
TTACGCCTGG
CCAACCGCCC
GTTCCTCCAC
ACATGGGGCA
TTCCACGGCG
TCGGTGCCTG
GTTGTTCTTG
ATTTTCCATG
CCGCCTCCTT
TAGCCGCTAA
AATTCATCTA
CTCATTTATT
CATTTGCTCA
TTTACTCTGG
TAGCTGCGCG
ATGTATTCAG
ATAGCAGCTC
GGTAATGGTC
TTGCCTTGGC
GTACCGCGTA
CATCTTCAGC
TTGGTGTGAT
CCTCCGCCGG
CAACTGAAAG
TTGACCCGCT
TCATGGCTGG
CGTGTCTGCC
AGGCTGGCCA
ACGTTGCAGC
CTTGCTGCTG
CGTGCGCTCG
GACGGCCGGC
ACTTAGCGTG
TTTGTGCTTT
TGCTCATTTT
CTCTTTACCT
CATTAACTCA
AATGAGTTTT
GATTTAATTT
CAGCGGCCAG
CGCCTGGACC
TCGCGGGCAG
CGTCGCCCTC
GGGTTCTGAT
TCAAGAACGG
TTGTGCCGGC
GGCGGCAGTG
CCTGGGTAGC
TCACGCGCTG
CGTGATACGG
GACTCAAGAA
TGGGCAGCTC
GTACCCGGCC
AGCGCCTCGG
CAACCTCACC
GCCGATGCGC
GTGCCTTTGA
TCGCCCGCGA
CACGACAAAG
GCCGCTTGTA
GCCTTCCATC
CGTGACCTCA
ATGCGCTGCT
TAACCAGCTC
CACCAGGTCG
GCGGTGGCCC
ATATGTCGTA
AGGGCTTGGC
TGCACCGGAA
TCAGCACGAA
GTCGGCTGCC
TTGATCGCGG
ACACAGCCAA
GTCCGCCGCC
TGGGGCGCTC
CGTCGATCAC
TACGAAGTCG
CGCCGGCCGA
TGGCCTTCAC
GTCGCGGTCA
ATCGTCGGGC
GGTCGATGCC
GACAACGGTT
AGCGGTTGAT
CTTCCCGCAC
GGCCGCCCAA
TCGCGGGCAC
TGCCCTGGGG
ATCGGAATCG
ACTAACAGAA
CATCGGCCCC
GGCGAGTTGC
AGGGCGCGGG
CTAGATGGGT
TGCGATGGTC
GTCTTGCCTG
ACCCGCCTTT
CTGGTTAAGT
ACAGCGATAA
CCTTCATGCG
TTCCCCTTGC
GTATTTGTTT
ATTTACTCAT
CGCATCATAT
ACGCAGCGAC
CGCATGACGC
AAGCTGTTTT
ACTCAAATAC
ACATCACCTT
TTTAGACGGC
GGCGCTCGGT
TTCTTCAGCG
GCCAAGCTGG
CCGGCCAGGC
CGCCAGCTTG
GCATCAGACA
AACCGGCCAG
GATTTCATGC
AGCCGCACGG
TTGAGACGTG
CGCGGGCGGC
TCGAACACGT
ACCCGGCCGC
GATCATCTCC
GCCTCGATCT
CTTCGGTAAT
GAAAAACGGT
TCGTCCTGGC
CGTCCTGGTG
CGGTTTCATG
CTTGTTCCTC
TTGGCGTTCA
TTCTCGGCGG
CCGCCAGGGC
GTCGGCCTCG
GTCAATGCGT
CCTCACGGAA
GGCACCGCGC
CGCCTGGCCT
CGGTGGGCGT
CACTTCCTCG
CTGCGCTCAA
GTGCGCGGTA
CAGGGTCGAG
CGATGCACGC
CAAGCAGTGC
AGCCGCCTCT
TTCACGGTGC
GGCCTTCCTG
GTCGATCAGC
TCGCGGGCGT
GCGCGATCTG
TGCCGGGGTG
AGGGTAGGGC
GGGGGCCAAA
CTTCACGCCT
CGGGCCTTGG
CGGCCTCGCG
CCCGCTCCGG
GTGCGGTCGA
TGATTAGGGA
ACGCTCGAAC
TCGGCAATGC
CGGCGAACAC
GGTCAACACC
ATGCGGCCGG
CCGGCGTGGT
GGTGTCGGCC
CACGGCTCTG
CCAGGCTACG
CAGGCCCGCG
CCGGCCTCCT
GGATGCGCTC
GGCAATGTCC
AGTAGGTCGC
GGGTGCTGCG
GGCCAGGCGG
TCTAGCCTGG
TCACTGTCAC
AACGTCGCCA
GGGCGTAGGT
GGTCAAGCAT
CCTGGCCAGC
TCCGGGCGGT
CGCGCCTGGT
GCCGGTGATC
TTCTCGGAAA
ACAGCTTGGT
GCAGCCGGCC
GCGTGCAGTT
CGGCCCGTTG
GTTGGTCAAG
TCCTGGTCGT
CGGTGCTGAC
GCGGGCATAG
CCCAGCAGGC
CAGCGGCGGC
GCTCTTGTTC
ATGGCGTAAT
GTCTCCGGTT
CTAGTCGCAA
GTATTCTACT
TTATGCGACT
AAAACACGCG
ACAAGAAAAC
GCCAGGAAAA
GGGCAGGGCG
GCAGCCTGTC
GCGTAACTTA
GGACTTGTGC
GACATGTCGT
TTTCAGAAGA
CGGCTGCACT
GAACGTCAGA
AGCCGACTGC
ACTATAGCAG
CGGAGGGGTT
GGATCAAAGT
ACTTTGATCC
CGAGGGGAAC
CCTGTGGTTG
GCATGCACAT
ACAAATGGAC
GAACGGATAA
ACCTTTTCAC
GCCCTTTTAA
ATATCCGATT
ATTCTAATAA
ACGCTCTTTT
CTCTTAGGTT
TACCCGCCAA
TATATCCTGT
CAAACACTGA
TAGTTTAAAC
TGAAGGCGGG
AAACGACAAT
CTGATCCAAG
CTCAAGCTGC
TCTAGCATTC
GCCATTCAGG
CTGCGCAACT
GTTGGGAAGG
GCGATCGGTG
CGGGCCTCTT
CGCTATTACG
CCAGCTGGCG
AAAGGGGGAT
GTGCTGCAAG
GCGATTAAGT
TGGGTAACGC
CAGGGTTTTC
CCAGTCACGA
CGTTGTAAAA
CGACGGCCAG
TGCCAAGCTT
GATTACGAAT
TCGAGCTCGG
TACCCGACGA
GTCAGTAATA
AACGGCGTCA
AAGTGGTTGC
AGCCGGCACA
CACGAGTCGT
GTTTATCAAC
TCAAAGCACA
AATACTTTTC
CTCAACCTAA
AAATAAGGCA
ATTAGCCAAA
AACAACTTTG
CGTGTAAACA
ACGCTCAATA
CACGTGTCAT
TTTATTATTA
GCTATTGCTT
CACCGCCTTA
GCTTTCTCGT
GACCTAGTCG
TCCTCGTCTT
TTCTTCTTCT
TCTTCTATAA
AACAATACCC
AAAGAGCTCT
TCTTCTTCAC
AATTCAGATT
TCAATTTCTC
AAAATCTTAA
AAACTTTCTC
TCAATTCTCT
CTACCGTGAT
CAAGGTAAAT
TTCTGTGTTC
CTTATTCTCT
CAAAATCTTC
GATTTTGTTT
TCGTTCGATC
CCAATTTCGT
ATATGTTCTT
TGGTTTAGAT
TCTGTTAATC
TTAGATCGAA
GACGATTTTC
TGGGTTTGAT
CGTTAGATAT
CATCTTAATT
CTCGATTAGG
GTTTCATAGA
TATCATCCGA
TTTGTTCAAA
TAATTTGAGT
TTTGTCGAAT
AATTACTCTT
CGATTTGTGA
TTTCTATCTA
GATCTGGTGT
TAGTTTCTAG
TTTGTGCGAT
CGAATTTGTA
GATTAATCTG
AGTTTTTCTG
ATTAACAGCT
CGAGTGCGGG
CCCCCCCTCG
AGGCGCGCCA
AGCTATCAAA
CAAGTTTGTA
CAAAAAAGCA
GGCTACCATA
TGGCCAGCAG
CCCCCCCAAG
AAGAAGCGCA
AGGTCGACAA
GAAGTACTCC
ATCGGCCTGG
ACATCGGCAC
GAACAGCGTG
GGCTGGGCCG
TGATCACCGA
CGAGTACAAG
GTGCCGTCCA
AGAAGTTCAA
GGTGCTGGGC
AACACCGACC
GCCACAGCAT
CAAGAAGAAC
CTGATCGGCG
CCCTGCTGTT
CGACTCGGGC
GAGACCGCCG
AGGCGACGCG
CCTGAAGCGC
ACCGCGCGCC
GCCGCTACAC
GCGCCGCAAG
AACCGCATCT
GCTACCTGCA
GGAGATCTTC
AGCAACGAGA
TGGCCAAGGT
GGACGACTCG
TTCTTCCACC
GCCTGGAGGA
GTCCTTCCTG
GTGGAGGAGG
ACAAGAAGCA
CGAGCGCCAC
CCCATCTTCG
GCAACATCGT
GGACGAGGTG
GCCTACCACG
AGAAGTACCC
GACGATCTAC
CACCTGCGCA
AGAAGCTGGT
GGACAGCACC
GACAAGGCGG
ACCTGCGCCT
GATCTACCTG
GCCCTGGCGC
ACATGATCAA
GTTCCGCGGC
CACTTCCTGA
TCGAGGGCGA
CCTGAACCCC
GACAACTCGG
ACGTGGACAA
GCTGTTCATC
CAGCTGGTGC
AGACCTACAA
CCAGCTGTTC
GAGGAGAACC
CGATCAACGC
CTCGGGCGTG
GACGCCAAGG
CGATCCTGTC
CGCGCGCCTG
TCCAAGAGCC
GCCGCTTGGA
GAACCTGATC
GCCCAGCTGC
CCGGCGAGAA
GAAGAACGGC
CTGTTCGGCA
ACCTGATCGC
GCTGAGCCTG
GGCCTGACGC
CGAACTTCAA
GTCGAACTTC
GACCTGGCCG
AGGACGCGAA
GCTGCAGCTG
AGCAAGGACA
CCTACGACGA
CGACCTGGAC
AACCTGCTGG
CCCAGATCGG
CGACCAGTAC
GCGGACCTGT
TCCTGGCCGC
GAAGAACCTG
TCGGACGCCA
TCCTGCTGTC
CGACATCCTG
CGCGTGAACA
CCGAGATCAC
GAAGGCCCCC
CTGTCGGCGT
CCATGATCAA
GCGCTACGAC
GAGCACCACC
AGGACCTGAC
CCTGCTGAAG
GCGCTGGTGC
GCCAGCAGCT
GCCGGAGAAG
TACAAGGAGA
TCTTCTTCGA
CCAGTCCAAG
AACGGCTACG
CCGGCTACAT
CGACGGCGGC
GCCAGCCAGG
AGGAGTTCTA
CAAGTTCATC
AAGCCCATCC
TGGAGAAGAT
GGACGGCACG
GAGGAGCTGC
TGGTGAAGCT
GAACCGCGAG
GACCTGCTGC
GCAAGCAGCG
CACCTTCGAC
AACGGCAGCA
TCCCCCACCA
GATCCACCTG
GGCGAGCTGC
ACGCCATCCT
GCGCCGCCAG
GAGGACTTCT
ACCCGTTCCT
GAAGGACAAC
CGCGAGAAGA
TCGAGAAGAT
CCTGACGTTC
CGCATCCCCT
ACTACGTGGG
CCCGCTGGCT
CGCGGCAACT
CCCGCTTCGC
GTGGATGACC
CGCAAGAGCG
AGGAGACCAT
CACGCCCTGG
AACTTCGAGG
AGGTGGTGGA
CAAGGGCGCC
AGCGCGCAGT
CGTTCATCGA
GCGCATGACC
AACTTCGACA
AGAACCTGCC
CAACGAGAAG
GTGCTGCCGA
AGCACTCCCT
GCTGTACGAG
TACTTCACCG
TGTACAACGA
GCTGACGAAG
GTGAAGTACG
TGACCGAGGG
CATGCGCAAG
CCCGCCTTCC
TGAGCGGCGA
GCAGAAGAAG
GCGATCGTGG
ACCTGCTGTT
CAAGACCAAC
CGCAAGGTGA
CGGTGAAGCA
GCTGAAGGAG
GACTACTTCA
AGAAGATCGA
GTGCTTCGAC
AGCGTGGAGA
TCTCGGGCGT
GGAGGACCGC
TTCAACGCCA
GCCTGGGCAC
CTACCACGAC
CTGCTGAAGA
TCATCAAGGA
CAAGGACTTC
CTGGACAACG
AGGAGAACGA
GGACATCCTG
GAGGACATCG
TGCTGACCCT
GACGCTGTTC
GAGGACCGCG
AGATGATCGA
GGAGCGCCTG
AAGACGTACG
CCCACCTGTT
CGACGACAAG
GTGATGAAGC
AGCTGAAGCG
CCGCCGCTAC
ACCGGCTGGG
GCCGCCTGAG
CCGCAAGCTG
ATCAACGGCA
TCCGCGACAA
GCAGTCGGGC
AAGACCATCC
TGGACTTCCT
GAAGTCCGAC
GGCTTCGCGA
ACCGCAACTT
CATGCAGCTG
ATCCACGACG
ACTCGCTGAC
CTTCAAGGAG
GACATCCAGA
AGGCCCAGGT
GTCGGGCCAG
GGCGACTCCC
TGCACGAGCA
CATCGCCAAC
CTGGCGGGCT
CCCCCGCGAT
CAAGAAGGGC
ATCCTGCAGA
CCGTGAAGGT
GGTGGACGAG
CTGGTGAAGG
TGATGGGCCG
CCACAAGCCG
GAGAACATCG
TGATCGAGAT
GGCCCGCGAG
AACCAGACCA
CGCAGAAGGG
CCAGAAGAAC
AGCCGCGAGC
GCATGAAGCG
CATCGAGGAG
GGCATCAAGG
AGCTGGGCTC
GCAGATCCTG
AAGGAGCACC
CCGTGGAGAA
CACCCAGCTG
CAGAACGAGA
AGCTGTACCT
GTACTACCTG
CAGAACGGCC
GCGACATGTA
CGTGGACCAG
GAGCTGGACA
TCAACCGCCT
GTCCGACTAC
GACGTGGACC
ACATCGTGCC
CCAGAGCTTC
CTGAAGGACG
ACTCGATCGA
CAACAAGGTG
CTGACCCGCA
GCGACAAGAA
CCGCGGCAAG
AGCGACAACG
TGCCGTCGGA
GGAGGTGGTG
AAGAAGATGA
AGAACTACTG
GCGCCAGCTG
CTGAACGCCA
AGCTGATCAC
GCAGCGCAAG
TTCGACAACC
TGACCAAGGC
CGAGCGCGGC
GGCCTGTCGG
AGCTGGACAA
GGCGGGCTTC
ATCAAGCGCC
AGCTGGTGGA
GACCCGCCAG
ATCACGAAGC
ACGTGGCGCA
GATCCTGGAC
TCCCGCATGA
ACACGAAGTA
CGACGAGAAC
GACAAGCTGA
TCCGCGAGGT
GAAGGTGATC
ACCCTGAAGT
CCAAGCTGGT
CAGCGACTTC
CGCAAGGACT
TCCAGTTCTA
CAAGGTGCGC
GAGATCAACA
ACTACCACCA
CGCCCACGAC
GCGTACCTGA
ACGCCGTGGT
GGGCACCGCG
CTGATCAAGA
AGTACCCCAA
GCTGGAGAGC
GAGTTCGTGT
ACGGCGACTA
CAAGGTGTAC
GACGTGCGCA
AGATGATCGC
CAAGTCGGAG
CAGGAGATCG
GCAAGGCCAC
CGCGAAGTAC
TTCTTCTACT
CCAACATCAT
GAACTTCTTC
AAGACCGAGA
TCACGCTGGC
CAACGGCGAG
ATCCGCAAGC
GCCCCCTGAT
CGAGACCAAC
GGCGAGACGG
GCGAGATCGT
GTGGGACAAG
GGCCGCGACT
TCGCGACCGT
GCGCAAGGTG
CTGAGCATGC
CCCAGGTGAA
CATCGTGAAG
AAGACCGAGG
TGCAGACGGG
CGGCTTCTCC
AAGGAGAGCA
TCCTGCCGAA
GCGCAACTCG
GACAAGCTGA
TCGCCCGCAA
GAAGGACTGG
GACCCCAAGA
AGTACGGCGG
CTTCGACTCC
CCGACCGTGG
CCTACAGCGT
GCTGGTGGTG
GCGAAGGTGG
AGAAGGGCAA
GTCCAAGAAG
CTGAAGAGCG
TGAAGGAGCT
GCTGGGCATC
ACCATCATGG
AGCGCAGCTC
GTTCGAGAAG
AACCCCATCG
ACTTCCTGGA
GGCGAAGGGC
TACAAGGAGG
TGAAGAAGGA
CCTGATCATC
AAGCTGCCGA
AGTACTCGCT
GTTCGAGCTG
GAGAACGGCC
GCAAGCGCAT
GCTGGCCTCC
GCGGGCGAGC
TGCAGAAGGG
CAACGAGCTG
GCCCTGCCCA
GCAAGTACGT
GAACTTCCTG
TACCTGGCGT
CCCACTACGA
GAAGCTGAAG
GGCTCGCCGG
AGGACAACGA
GCAGAAGCAG
CTGTTCGTGG
AGCAGCACAA
GCACTACCTG
GACGAGATCA
TCGAGCAGAT
CTCGGAGTTC
TCCAAGCGCG
TGATCCTGGC
CGACGCGAAC
CTGGACAAGG
TGCTGAGCGC
CTACAACAAG
CACCGCGACA
AGCCCATCCG
CGAGCAGGCG
GAGAACATCA
TCCACCTGTT
CACCCTGACG
AACCTGGGCG
CCCCGGCCGC
GTTCAAGTAC
TTCGACACCA
CGATCGACCG
CAAGCGCTAC
ACCAGCACGA
AGGAGGTGCT
GGACGCGACC
CTGATCCACC
AGAGCATCAC
CGGCCTGTAC
GAGACGCGCA
TCGACCTGTC
GCAGCTGGGC
GGCGACTAAT
GTACCCAGCT
TTCTTGTACA
AAGTGGTTCG
ATAATTCTTA
ATTAACTAGT
TCTAGAGCGG
CCGCCACCGC
GGTGGAGCTC
GAATTTCCCC
GATCGTTCAA
ACATTTGGCA
ATAAAGTTTC
TTAAGATTGA
ATCCTGTTGC
CGGTCTTGCG
ATGATTATCA
TATAATTTCT
GTTGAATTAC
GTTAAGCATG
TAATAATTAA
CATGTAATGC
ATGACGTTAT
TTATGAGATG
GGTTTTTATG
ATTAGAGTCC
CGCAATTATA
CATTTAATAC
GCGATAGAAA
ACAAAATATA
GCGCGCAAAC
TAGGATAAAT
TATCGCGCGC
GGTGTCATCT
ATGTTACTAG
ATCGGGAA