名称: | pRGE32 |
---|---|
别称: | Plasmid#63159 |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 空载体 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | Ubi,Osu3 |
复制子: | pVS1 |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CTTGTACAAA
GTGGTTGATA
ACAGCGACTA
CAAGGATGAC
GATGACAAGG
CTTAGAGCTC
GAATTTCCCC
GATCGTTCAA
ACATTTGGCA
ATAAAGTTTC
TTAAGATTGA
ATCCTGTTGC
CGGTCTTGCG
ATGATTATCA
TATAATTTCT
GTTGAATTAC
GTTAAGCATG
TAATAATTAA
CATGTAATGC
ATGACGTTAT
TTATGAGATG
GGTTTTTATG
ATTAGAGTCC
CGCAATTATA
CATTTAATAC
GCGATAGAAA
ACAAAATATA
GCGCGCAAAC
TAGGATAAAT
TATCGCGCGC
GGTGTCATCT
ATGTTACTAG
ATCGGGAATT
CACTGGCCGT
CGTTTTACAA
CGTCGTGACT
GGGAAAACCC
TGGCGTTACC
CAACTTAATC
GCCTTGCAGC
ACATCCCCCT
TTCGCCAGCT
GGCGTAATAG
CGAAGAGGCC
CGCACCGATC
GCCCTTCCCA
ACAGTTGCGC
AGCCTGAATG
GCGAATGGCG
CCTGATGCGG
TATTTTCTCC
TTACGCATCT
GTGCGGTATT
TCACACCGCA
TACGTCAAAG
CAACCATAGT
ACGCGCCCTG
TAGCGGCGCA
TTAAGCGCGG
CGGGTGTGGT
GGTTACGCGC
AGCGTGACCG
CTACACTTGC
CAGCGCCCTA
GCGCCCGCTC
CTTTCGCTTT
CTTCCCTTCC
TTTCTCGCCA
CGTTCGCCGG
CTTTCCCCGT
CAAGCTCTAA
ATCGGGGGCT
CCCTTTAGGG
TTCCGATTTA
GTGCTTTACG
GCACCTCGAC
CCCAAAAAAC
TTGATTTGGG
TGATGGTTCA
CGTAGTGGGC
CATCGCCCTG
ATAGACGGTT
TTTCGCCCTT
TGACGTTGGA
GTCCACGTTC
TTTAATAGTG
GACTCTTGTT
CCAAACTGGA
ACAACACTCA
ACCCTATCTC
GGGCTATTCT
TTTGATTTAT
AAGGGATTTT
GCCGATTTCG
GCCTATTGGT
TAAAAAATGA
GCTGATTTAA
CAAAAATTTA
ACGCGAATTT
TAACAAAATA
TTAACGTTTA
CAATTTTATG
GTGCACTCTC
AGTACAATCT
GCTCTGATGC
CGCATAGTTA
AGCCAGCCCC
GACACCCGCC
AACACCCGCT
GACGCGCCCT
GACGGGCTTG
TCTGCTCCCG
GCATCCGCTT
ACAGACAAGC
TGTGACCGTC
TCCGGGAGCT
GCATGTGTCA
GAGGTTTTCA
CCGTCATCAC
CGAAACGCGC
GAGACGAAAG
GGCCTCGTGA
TACGCCTATT
TTTATAGGTT
AATGTCATGA
TAATAATGGT
TTCTTAGACG
TCAGGTGGCA
CTTTTCGGGG
AAATGTGCGC
GGAACCCCTA
TTTGTTTATT
TTTCTAAATA
CATTCAAATA
TGTATCCGCT
CATGAGACAA
TAACCCTGAT
AAATGCTTCA
ATAATATTGA
AAAAGGAAGA
GTATGAGTAT
TCAACATTTC
CGTGTCGCCC
TTATTCCCTT
TTTTGCGGCA
TTTTGCCTTC
CTGTTTTTGC
TCACCCAGAA
ACGCTGGTGA
AAGTAAAAGA
TGCTGAAGAT
CAGTTGGGTG
CACGAGTGGG
TTACATCGAA
CTGGATCTCA
ACAGCGGTAA
GATCCTTGAG
AGTTTTCGCC
CCGAAGAACG
TTTTCCAATG
ATGAGCACTT
TTAAAGTTCT
GCTATGTGGC
GCGGTATTAT
CCCGTATTGA
CGCCGGGCAA
GAGCAACTCG
GTCGCCGCAT
ACACTATTCT
CAGAATGACT
TGGTTGAGTA
CTCACCAGTC
ACAGAAAAGC
ATCTTACGGA
TGGCATGACA
GTAAGAGAAT
TATGCAGTGC
TGCCATAACC
ATGAGTGATA
ACACTGCGGC
CAACTTACTT
CTGACAACGA
TCGGAGGACC
GAAGGAGCTA
ACCGCTTTTT
TGCACAACAT
GGGGGATCAT
GTAACTCGCC
TTGATCGTTG
GGAACCGGAG
CTGAATGAAG
CCATACCAAA
CGACGAGCGT
GACACCACGA
TGCCTGTAGC
AATGGCAACA
ACGTTGCGCA
AACTATTAAC
TGGCGAACTA
CTTACTCTAG
CTTCCCGGCA
ACAATTAATA
GACTGGATGG
AGGCGGATAA
AGTTGCAGGA
CCACTTCTGC
GCTCGGCCCT
TCCGGCTGGC
TGGTTTATTG
CTGATAAATC
TGGAGCCGGT
GAGCGTGGCA
CTCGCGGTAT
CATTGCAGCA
CTGGGGCCAG
ATGGTAAGCC
CTCCCGTATC
GTAGTTATCT
ACACGACGGG
GAGTCAGGCA
ACTATGGATG
AACGAAATAG
ACAGATCGCT
GAGATAGGTG
CCTCACTGAT
TAAGCATTGG
TAACTGTCAG
ACCAAGTTTA
CTCATATATA
CTTTAGATTG
ATTTAAAACT
TCATTTTTAA
TTTAAAAGGA
TCTAGGTGAA
GATCCTTTTT
GATAATCTCA
TGACCAAAAT
CCCTTAACGT
GAGTTTTCGT
TCCACTGAGC
GTCAGACCCC
GTAGAAAAGA
TCAAAGGATC
TTCTTGAGAT
CCTTTTTTTC
TGCGCGTAAT
CTGCTGCTTG
CAAACAAAAA
AACCACCGCT
ACCAGCGGTG
GTTTGTTTGC
CGGATCAAGA
GCTACCAACT
CTTTTTCCGA
AGGTAACTGG
CTTCAGCAGA
GCGCAGATAC
CAAATACTGT
CCTTCTAGTG
TAGCCGTAGT
TAGGCCACCA
CTTCAAGAAC
TCTGTAGCAC
CGCCTACATA
CCTCGCTCTG
CTAATCCTGT
TACCAGTGGC
TGCTGCCAGT
GGCGATAAGT
CGTGTCTTAC
CGGGTTGGAC
TCAAGACGAT
AGTTACCGGA
TAAGGCGCAG
CGGTCGGGCT
GAACGGGGGG
TTCGTGCACA
CAGCCCAGCT
TGGAGCGAAC
GACCTACACC
GAACTGAGAT
ACCTACAGCG
TGAGCTATGA
GAAAGCGCCA
CGCTTCCCGA
AGGGAGAAAG
GCGGACAGGT
ATCCGGTAAG
CGGCAGGGTC
GGAACAGGAG
AGCGCACGAG
GGAGCTTCCA
GGGGGAAACG
CCTGGTATCT
TTATAGTCCT
GTCGGGTTTC
GCCACCTCTG
ACTTGAGCGT
CGATTTTTGT
GATGCTCGTC
AGGGGGGCGG
AGCCTATGGA
AAAACGCCAG
CAACGCGGCC
TTTTTACGGT
TCCTGGCCTT
TTGCTGGCCT
TTTGCTCACA
TGTTCTTTCC
TGCGTTATCC
CCTGATTCTG
TGGATAACCG
TATTACCGCC
TTTGAGTGAG
CTGATACCGC
TCGCCGCAGC
CGAACGACCG
AGCGCAGCGA
GTCAGTGAGC
GAGGAAGCGG
AAGAGCGCCC
AATACGCAAA
CCGCCTCTCC
CCGCGCGTTG
GCCGATTCAT
TAATGCAGCT
GGCACGACAG
GTTTCCCGAC
TGGAAAGCGG
GCAGTGAGCG
CAACGCAATT
AATGTGAGTT
AGCTCACTCA
TTAGGCACCC
CAGGCTTTAC
ACTTTATGCT
TCCGGCTCGT
ATGTTGTGTG
GAATTGTGAG
CGGATAACAA
TTTCACACAG
GAAACAGCTA
TGACCATGAT
TACGCCAAGC
TTAAGGAATC
TTTAAACATA
CGAACAGATC
ACTTAAAGTT
CTTCTGAAGC
AACTTAAAGT
TATCAGGCAT
GCATGGATCT
TGGAGGAATC
AGATGTGCAG
TCAGGGACCA
TAGCACAAGA
CAGGCGTCTT
CTACTGGTGC
TACCAGCAAA
TGCTGGAAGC
CGGGAACACT
GGGTACGTTG
GAAACCACGT
GATGTGAAGA
AGTAAGATAA
ACTGTAGGAG
AAAAGCATTT
CGTAGTGGGC
CATGAAGCCT
TTCAGGACAT
GTATTGCAGT
ATGGGCCGGC
CCATTACGCA
ATTGGACGAC
AACAAAGACT
AGTATTAGTA
CCACCTCGGC
TATCCACATA
GATCAAAGCT
GATTTAAAAG
AGTTGTGCAG
ATGATCCGTG
GCAGGAGACC
GAGGTCTCGG
TTTTAGAGCT
AGAAATAGCA
AGTTAAAATA
AGGCTAGTCC
GTTATCAACT
TGAAAAAGTG
GCACCGAGTC
GGTGCTTTTT
TGTTTTAGAG
CTAGAAATAG
CAAGTTAAAA
TAAGGCTAGT
CCGTTTTTAG
CGCGTGCATG
CCTGCAGGTC
CACAAATTCG
GGTCAAGGCG
GAAGCCAGCG
CGCCACCCCA
CGTCAGCAAA
TACGGAGGCG
CGGGGTTGAC
GGCGTCACCC
GGTCCTAACG
GCGACCAACA
AACCAGCCAG
AAGAAATTAC
AGTAAAAAAA
AAGTAAATTG
CACTTTGATC
CACCTTTTAT
TACCTAAGTC
TCAATTTGGA
TCACCCTTAA
ACCTATCTTT
TCAATTTGGG
CCGGGTTGTG
GTTTGGACTA
CCATGAACAA
CTTTTCGTCA
TGTCTAACTT
CCCTTTCAGC
AAACATATGA
ACCATATATA
GAGGAGATCG
GCCGTATACT
AGAGCTGATG
TGTTTAAGGT
CGTTGATTGC
ACGAGAAAAA
AAAATCCAAA
TCGCAACAAT
AGCAAATTTA
TCTGGTTCAA
AGTGAAAAGA
TATGTTTAAA
GGTAGTCCAA
AGTAAAACTT
ATAGATAATA
AAATGTGGTC
CAAAGCGTAA
TTCACTCAAA
AAAAATCAAC
GAGACGTGTA
CCAAACGGAG
ACAAACGGCA
TCTTCTCGAA
ATTTCCCAAC
CGCTCGCTCG
CCCGCCTCGT
CTTCCCGGAA
ACCGCGGTGG
TTTCAGCGTG
GCGGATTCTC
CAAGCAGACG
GAGACGTCAC
GGCACGGGAC
TCCTCCCACC
ACCCAACCGC
CATAAATACC
AGCCCCCTCA
TCTCCTCTCC
TCGCATCAGC
TCCACCCCCG
AAAAATTTCT
CCCCAATCTC
GCGAGGCTCT
CGTCGTCGAA
TCGAATCCTC
TCGCGTCCTC
AAGGTACGCT
GCTTCTCCTC
TCCTCGCTTC
GTTTCGATTC
GATTTCGGAC
GGGTGAGGTT
GTTTTGTTGC
TAGATCCGAT
TGGTGGTTAG
GGTTGTCGAT
GTGATTATCG
TGAGATGTTT
AGGGGTTGTA
GATCTGATGG
TTGTGATTTG
GGCACGGTTG
GTTCGATAGG
TGGAATCGTG
GTTAGGTTTT
GGGATTGGAT
GTTGGTTCTG
ATGATTGGGG
GGAATTTTTA
CGGTTAGATG
AATTGTTGGA
TGATTCGATT
GGGGAAATCG
GTGTAGATCT
GTTGGGGAAT
TGTGGAACTA
GTCATGCCTG
AGTGATTGGT
GCGATTTGTA
GCGTGTTCCA
TCTTGTAGGC
CTTGTTGCGA
GCATGTTCAG
ATCTACTGTT
CCGCTCTTGA
TTGAGTTATT
GGTGCCATGG
GTTGGTGCAA
ACACAGGCTT
TAATATGTTA
TATCTGTTTT
GTGTTTGATG
TAGATCTGTA
GGGTAGTTCT
TCTTAGACAT
GGTTCAATTA
TGTAGCTTGT
GCGTTTCGAT
TTGATTTCAT
ATGTTCACAG
ATTAGATAAT
GATGAACTCT
TTTAATTAAT
TGTCAATGGT
AAATAGGAAG
TCTTGTCGCT
ATATCTGTCA
TAATGATCTC
ATGTTACTAT
CTGCCAGTAA
TTTATGCTAA
GAACTATATT
AGAATATCAT
GTTACAATCT
GTAGTAATAT
CATGTTACAA
TCTGTAGTTC
ATCTATATAA
TCTATTGTGG
TAATTTCTTT
TTACTATCTG
TGTGAAGATT
ATTGCCACTA
GTTCATTCTA
CTTATTTCTG
AAGTTCAGGA
TACGTGTGCT
GTTACTACCT
ATCTGAATAC
ATGTGTGATG
TGCCTGTTAC
TATCTTTTTG
AATACATGTA
TGTTCTGTTG
GAATATGTTT
GCTGTTTGAT
CCGTTGTTGT
GTCCTTAATC
TTGTGCTAGT
TCTTACCCTA
TCTGTTTGGT
GATTATTTCT
TGCAGATAGT
TATCAACAAG
TTTGTACAAA
AAAGCAGGCT
TCGAAGGAGA
TAGAACCAAT
TCTCTAAGGA
AATACTTAAC
CATGGACTAT
AAGGACCACG
ACGGAGACTA
CAAGGATCAT
GATATTGATT
ACAAAGACGA
TGACGATAAG
ATGGCCCCAA
AGAAGAAGCG
GAAGGTCGGT
ATCCACGGAG
TCCCAGCAGC
CGACAAGAAG
TACAGCATCG
GCCTGGACAT
CGGCACCAAC
TCTGTGGGCT
GGGCCGTGAT
CACCGACGAG
TACAAGGTGC
CCAGCAAGAA
ATTCAAGGTG
CTGGGCAACA
CCGACCGGCA
CAGCATCAAG
AAGAACCTGA
TCGGAGCCCT
GCTGTTCGAC
AGCGGCGAAA
CAGCCGAGGC
CACCCGGCTG
AAGAGAACCG
CCAGAAGAAG
ATACACCAGA
CGGAAGAACC
GGATCTGCTA
TCTGCAAGAG
ATCTTCAGCA
ACGAGATGGC
CAAGGTGGAC
GACAGCTTCT
TCCACAGACT
GGAAGAGTCC
TTCCTGGTGG
AAGAGGATAA
GAAGCACGAG
CGGCACCCCA
TCTTCGGCAA
CATCGTGGAC
GAGGTGGCCT
ACCACGAGAA
GTACCCCACC
ATCTACCACC
TGAGAAAGAA
ACTGGTGGAC
AGCACCGACA
AGGCCGACCT
GCGGCTGATC
TATCTGGCCC
TGGCCCACAT
GATCAAGTTC
CGGGGCCACT
TCCTGATCGA
GGGCGACCTG
AACCCCGACA
ACAGCGACGT
GGACAAGCTG
TTCATCCAGC
TGGTGCAGAC
CTACAACCAG
CTGTTCGAGG
AAAACCCCAT
CAACGCCAGC
GGCGTGGACG
CCAAGGCCAT
CCTGTCTGCC
AGACTGAGCA
AGAGCAGACG
GCTGGAAAAT
CTGATCGCCC
AGCTGCCCGG
CGAGAAGAAG
AATGGCCTGT
TCGGAAACCT
GATTGCCCTG
AGCCTGGGCC
TGACCCCCAA
CTTCAAGAGC
AACTTCGACC
TGGCCGAGGA
TGCCAAACTG
CAGCTGAGCA
AGGACACCTA
CGACGACGAC
CTGGACAACC
TGCTGGCCCA
GATCGGCGAC
CAGTACGCCG
ACCTGTTTCT
GGCCGCCAAG
AACCTGTCCG
ACGCCATCCT
GCTGAGCGAC
ATCCTGAGAG
TGAACACCGA
GATCACCAAG
GCCCCCCTGA
GCGCCTCTAT
GATCAAGAGA
TACGACGAGC
ACCACCAGGA
CCTGACCCTG
CTGAAAGCTC
TCGTGCGGCA
GCAGCTGCCT
GAGAAGTACA
AAGAGATTTT
CTTCGACCAG
AGCAAGAACG
GCTACGCCGG
CTACATTGAC
GGCGGAGCCA
GCCAGGAAGA
GTTCTACAAG
TTCATCAAGC
CCATCCTGGA
AAAGATGGAC
GGCACCGAGG
AACTGCTCGT
GAAGCTGAAC
AGAGAGGACC
TGCTGCGGAA
GCAGCGGACC
TTCGACAACG
GCAGCATCCC
CCACCAGATC
CACCTGGGAG
AGCTGCACGC
CATTCTGCGG
CGGCAGGAAG
ATTTTTACCC
ATTCCTGAAG
GACAACCGGG
AAAAGATCGA
GAAGATCCTG
ACCTTCCGCA
TCCCCTACTA
CGTGGGCCCT
CTGGCCAGGG
GAAACAGCAG
ATTCGCCTGG
ATGACCAGAA
AGAGCGAGGA
AACCATCACC
CCCTGGAACT
TCGAGGAAGT
GGTGGACAAG
GGCGCTTCCG
CCCAGAGCTT
CATCGAGCGG
ATGACCAACT
TCGATAAGAA
CCTGCCCAAC
GAGAAGGTGC
TGCCCAAGCA
CAGCCTGCTG
TACGAGTACT
TCACCGTGTA
TAACGAGCTG
ACCAAAGTGA
AATACGTGAC
CGAGGGAATG
AGAAAGCCCG
CCTTCCTGAG
CGGCGAGCAG
AAAAAGGCCA
TCGTGGACCT
GCTGTTCAAG
ACCAACCGGA
AAGTGACCGT
GAAGCAGCTG
AAAGAGGACT
ACTTCAAGAA
AATCGAGTGC
TTCGACTCCG
TGGAAATCTC
CGGCGTGGAA
GATCGGTTCA
ACGCCTCCCT
GGGCACATAC
CACGATCTGC
TGAAAATTAT
CAAGGACAAG
GACTTCCTGG
ACAATGAGGA
AAACGAGGAC
ATTCTGGAAG
ATATCGTGCT
GACCCTGACA
CTGTTTGAGG
ACAGAGAGAT
GATCGAGGAA
CGGCTGAAAA
CCTATGCCCA
CCTGTTCGAC
GACAAAGTGA
TGAAGCAGCT
GAAGCGGCGG
AGATACACCG
GCTGGGGCAG
GCTGAGCCGG
AAGCTGATCA
ACGGCATCCG
GGACAAGCAG
TCCGGCAAGA
CAATCCTGGA
TTTCCTGAAG
TCCGACGGCT
TCGCCAACAG
AAACTTCATG
CAGCTGATCC
ACGACGACAG
CCTGACCTTT
AAAGAGGACA
TCCAGAAAGC
CCAGGTGTCC
GGCCAGGGCG
ATAGCCTGCA
CGAGCACATT
GCCAATCTGG
CCGGCAGCCC
CGCCATTAAG
AAGGGCATCC
TGCAGACAGT
GAAGGTGGTG
GACGAGCTCG
TGAAAGTGAT
GGGCCGGCAC
AAGCCCGAGA
ACATCGTGAT
CGAAATGGCC
AGAGAGAACC
AGACCACCCA
GAAGGGACAG
AAGAACAGCC
GCGAGAGAAT
GAAGCGGATC
GAAGAGGGCA
TCAAAGAGCT
GGGCAGCCAG
ATCCTGAAAG
AACACCCCGT
GGAAAACACC
CAGCTGCAGA
ACGAGAAGCT
GTACCTGTAC
TACCTGCAGA
ATGGGCGGGA
TATGTACGTG
GACCAGGAAC
TGGACATCAA
CCGGCTGTCC
GACTACGATG
TGGACCATAT
CGTGCCTCAG
AGCTTTCTGA
AGGACGACTC
CATCGACAAC
AAGGTGCTGA
CCAGAAGCGA
CAAGAACCGG
GGCAAGAGCG
ACAACGTGCC
CTCCGAAGAG
GTCGTGAAGA
AGATGAAGAA
CTACTGGCGG
CAGCTGCTGA
ACGCCAAGCT
GATTACCCAG
AGAAAGTTCG
ACAATCTGAC
CAAGGCCGAG
AGAGGCGGCC
TGAGCGAACT
GGATAAGGCC
GGCTTCATCA
AGAGACAGCT
GGTGGAAACC
CGGCAGATCA
CAAAGCACGT
GGCACAGATC
CTGGACTCCC
GGATGAACAC
TAAGTACGAC
GAGAATGACA
AGCTGATCCG
GGAAGTGAAA
GTGATCACCC
TGAAGTCCAA
GCTGGTGTCC
GATTTCCGGA
AGGATTTCCA
GTTTTACAAA
GTGCGCGAGA
TCAACAACTA
CCACCACGCC
CACGACGCCT
ACCTGAACGC
CGTCGTGGGA
ACCGCCCTGA
TCAAAAAGTA
CCCTAAGCTG
GAAAGCGAGT
TCGTGTACGG
CGACTACAAG
GTGTACGACG
TGCGGAAGAT
GATCGCCAAG
AGCGAGCAGG
AAATCGGCAA
GGCTACCGCC
AAGTACTTCT
TCTACAGCAA
CATCATGAAC
TTTTTCAAGA
CCGAGATTAC
CCTGGCCAAC
GGCGAGATCC
GGAAGCGGCC
TCTGATCGAG
ACAAACGGCG
AAACCGGGGA
GATCGTGTGG
GATAAGGGCC
GGGATTTTGC
CACCGTGCGG
AAAGTGCTGA
GCATGCCCCA
AGTGAATATC
GTGAAAAAGA
CCGAGGTGCA
GACAGGCGGC
TTCAGCAAAG
AGTCTATCCT
GCCCAAGAGG
AACAGCGATA
AGCTGATCGC
CAGAAAGAAG
GACTGGGACC
CTAAGAAGTA
CGGCGGCTTC
GACAGCCCCA
CCGTGGCCTA
TTCTGTGCTG
GTGGTGGCCA
AAGTGGAAAA
GGGCAAGTCC
AAGAAACTGA
AGAGTGTGAA
AGAGCTGCTG
GGGATCACCA
TCATGGAAAG
AAGCAGCTTC
GAGAAGAATC
CCATCGACTT
TCTGGAAGCC
AAGGGCTACA
AAGAAGTGAA
AAAGGACCTG
ATCATCAAGC
TGCCTAAGTA
CTCCCTGTTC
GAGCTGGAAA
ACGGCCGGAA
GAGAATGCTG
GCCTCTGCCG
GCGAACTGCA
GAAGGGAAAC
GAACTGGCCC
TGCCCTCCAA
ATATGTGAAC
TTCCTGTACC
TGGCCAGCCA
CTATGAGAAG
CTGAAGGGCT
CCCCCGAGGA
TAATGAGCAG
AAACAGCTGT
TTGTGGAACA
GCACAAGCAC
TACCTGGACG
AGATCATCGA
GCAGATCAGC
GAGTTCTCCA
AGAGAGTGAT
CCTGGCCGAC
GCTAATCTGG
ACAAAGTGCT
GTCCGCCTAC
AACAAGCACC
GGGATAAGCC
CATCAGAGAG
CAGGCCGAGA
ATATCATCCA
CCTGTTTACC
CTGACCAATC
TGGGAGCCCC
TGCCGCCTTC
AAGTACTTTG
ACACCACCAT
CGACCGGAAG
AGGTACACCA
GCACCAAAGA
GGTGCTGGAC
GCCACCCTGA
TCCACCAGAG
CATCACCGGC
CTGTACGAGA
CACGGATCGA
CCTGTCTCAG
CTGGGAGGCG
ACAAAAGGCC
GGCGGCCACG
AAAAAGGCCG
GCCAGGCAAA
AAAGAAAAAG
TAAGAATTCG
CGGCCGCACT
CGAGATATCT
AGACCCAGCT
TT