产品货号：P10603
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pEGB2Ω2_SF-35s:hCas9:tNos (GB1103) |
|---|---|
| 别称: | Plasmid#75400 |
| 基因长: | |
| 质粒宿主： | 植物 |
| 质粒用途： | 基因编辑 |
| 片段类型： | CRISPR |
| 片段物种： | |
| 原核抗性： | Spe |
| 筛选标记： | |
| 荧光标记： | |
| 启动子: | 35S |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | DH5a |
| 温度: | 37度 |
| 背景载体: | |
| 正向引物: | |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
GGAGTCTCAA
TGGTAACTTT
ACTCTTTATT
TAACCATACA
TTTTTTTTTA
TTTTTTTCAC
TTTGTTCTTC
ATCCACTATT
GTTCTTTGTT
CATCTTGAAC
AAAAGCTCCC
TCCTTCTTTG
TTCTTCATCC
ACCATTGTTC
TTCATCAATC
ATTTCGCTGT
CAGGAGACTA
GAGCCAAGCT
GATCTCCTTT
GCCCCGGAGA
TCACCATGGA
CGACTTTCTC
TATCTCTACG
ATCTAGGAAG
AAAGTTCGAC
GGAGAAGGTG
ACGATACCAT
GTTCACCACC
GATAATGAGA
AGATTAGCCT
CTTCAATTTC
AGAAAGAATG
CTGACCCACA
GATGGTTAGA
GAGGCCTACG
CGGCAGGTCT
GATCAAGACG
ATCTACCCGA
GTAATAATCT
CCAGGAGATC
AAATACCTTC
CCAAGAAGGT
TAAAGATGCA
GTCAAAAGAT
TCAGGACTAA
CTGCATCAAG
AACACAGAGA
AAGATATATT
TCTCAAGATC
AGAAGTACTA
TTCCAGTATG
GACGATTCAA
GGCTTGCTTC
ATAAACCAAG
GCAAGTAATA
GAGATTGGAG
TCTCTAAGAA
AGTAGTTCCT
ACTGAATCAA
AGGCCATGGA
GTCAAAAATT
CAGATCGAGG
ATCTAACAGA
ACTCGCCGTG
AAGACTGGCG
AACAGTTCAT
ACAGAGTCTT
TTACGACTCA
ATGACAAGAA
GAAAATCTTC
GTCAACATGG
TGGAGCACGA
CACTCTCGTC
TACTCCAAGA
ATATCAAAGA
TACAGTCTCA
GAAGACCAAA
GGGCTATTGA
GACTTTTCAA
CAAAGGGTAA
TATCGGGAAA
CCTCCTCGGA
TTCCATTGCC
CAGCTATCTG
TCACTTCATC
AAAAGGACAG
TAGAAAAGGA
AGGTGGCACC
TACAAATGCC
ATCATTGCGA
TAAAGGAAAG
GCTATCGTTC
AAGATGCCCC
TGCCGACAGT
GGTCCCAAAG
ATGGACCCCC
ACCCACGAGG
AGCATCGTGG
AAAAAGAAGA
CGTTCCAACC
ACGTCTTCAA
AGCAAGTGGA
TTGATGTGAT
ATCTCCACTG
ACGTAAGGGA
TGACGCACAA
TCCCACTATC
CTTCGCAAGA
CCCTTCCTCT
ATATAAGGAA
GTTCATTTCA
TTTGGAGAGG
ACTCCGGTAT
TTTTACAACA
ATTACCACAA
CAAAACAAAC
AACAAACAAC
ATTACAATTT
ACTATTCTAG
TCGAAATGGA
CAAGAAGTAC
TCCATTGGGC
TCGATATCGG
CACAAACAGC
GTCGGCTGGG
CCGTCATTAC
GGACGAGTAC
AAGGTGCCGA
GCAAAAAATT
CAAAGTTCTG
GGCAATACCG
ATCGCCACAG
CATAAAGAAG
AACCTCATTG
GCGCCCTCCT
GTTCGACTCC
GGGGAAACGG
CCGAAGCCAC
GCGGCTCAAA
AGAACAGCAC
GGCGCAGATA
TACCCGCAGA
AAGAATCGGA
TCTGCTACCT
GCAGGAGATC
TTTAGTAATG
AGATGGCTAA
GGTGGATGAC
TCTTTCTTCC
ATAGGCTGGA
GGAGTCCTTT
TTGGTGGAGG
AGGATAAAAA
GCACGAGCGC
CACCCAATCT
TTGGCAATAT
CGTGGACGAG
GTGGCGTACC
ATGAAAAGTA
CCCAACCATA
TATCATCTGA
GGAAGAAGCT
TGTAGACAGT
ACTGATAAGG
CTGACTTGCG
GTTGATCTAT
CTCGCGCTGG
CGCATATGAT
CAAATTTCGG
GGACACTTCC
TCATCGAGGG
GGACCTGAAC
CCAGACAACA
GCGATGTCGA
CAAACTCTTT
ATCCAACTGG
TTCAGACTTA
CAATCAGCTT
TTCGAAGAGA
ACCCGATCAA
CGCATCCGGA
GTTGACGCCA
AAGCAATCCT
GAGCGCTAGG
CTGTCCAAAT
CCCGGCGGCT
CGAAAACCTC
ATCGCACAGC
TCCCTGGGGA
GAAGAAGAAC
GGCCTGTTTG
GTAATCTTAT
CGCCCTGTCA
CTCGGGCTGA
CCCCCAACTT
TAAATCTAAC
TTCGACCTGG
CCGAAGATGC
CAAGCTTCAA
CTGAGCAAAG
ACACCTACGA
TGATGATCTC
GACAATCTGC
TGGCCCAGAT
CGGCGACCAG
TACGCAGACC
TTTTTTTGGC
GGCAAAGAAC
CTGTCAGACG
CCATTCTGCT
GAGTGATATT
CTGCGAGTGA
ACACGGAGAT
CACCAAAGCT
CCGCTGAGCG
CTAGTATGAT
CAAGCGCTAT
GATGAGCACC
ACCAAGACTT
GACTTTGCTG
AAGGCCCTTG
TCAGACAGCA
ACTGCCTGAG
AAGTACAAGG
AAATTTTCTT
CGATCAGTCT
AAAAATGGCT
ACGCCGGATA
CATTGACGGC
GGAGCAAGCC
AGGAGGAATT
TTACAAATTT
ATTAAGCCCA
TCTTGGAAAA
AATGGACGGC
ACCGAGGAGC
TGCTGGTAAA
GCTTAACAGA
GAAGATCTGT
TGCGCAAACA
GCGCACTTTC
GACAATGGAA
GCATCCCCCA
CCAGATTCAC
CTGGGCGAAC
TGCACGCTAT
CCTCAGGCGG
CAAGAGGATT
TCTACCCCTT
TTTGAAAGAT
AACAGGGAAA
AGATTGAGAA
AATCCTCACA
TTTCGGATAC
CCTACTATGT
AGGCCCCCTC
GCCCGGGGAA
ATTCCAGATT
CGCGTGGATG
ACTCGCAAAT
CAGAAGAGAC
TATCACTCCC
TGGAACTTCG
AGGAAGTCGT
GGATAAGGGG
GCCTCTGCCC
AGTCCTTCAT
CGAAAGGATG
ACTAACTTTG
ATAAAAATCT
GCCTAACGAA
AAGGTGCTTC
CTAAACACTC
TCTGCTGTAC
GAGTACTTCA
CAGTTTATAA
CGAGCTCACC
AAGGTCAAAT
ACGTCACAGA
AGGGATGAGA
AAGCCAGCAT
TCCTGTCTGG
AGAGCAGAAG
AAAGCTATCG
TGGACCTCCT
CTTCAAGACG
AACCGGAAAG
TTACCGTGAA
ACAGCTCAAA
GAAGATTATT
TCAAAAAGAT
TGAATGTTTC
GACTCTGTTG
AAATCAGCGG
AGTGGAGGAT
CGCTTCAACG
CATCCCTGGG
AACGTATCAC
GATCTCCTGA
AAATCATTAA
AGACAAGGAC
TTCCTGGACA
ATGAGGAGAA
CGAGGACATT
CTTGAGGACA
TTGTCCTCAC
CCTTACGTTG
TTTGAAGATA
GGGAGATGAT
TGAAGAACGC
TTGAAAACTT
ACGCTCATCT
CTTCGACGAC
AAAGTCATGA
AACAGCTCAA
GAGGCGCCGA
TATACAGGAT
GGGGGCGGCT
GTCAAGAAAA
CTGATCAATG
GGATCCGAGA
CAAGCAGAGT
GGAAAGACAA
TCCTGGATTT
TCTTAAGTCC
GATGGATTTG
CCAACCGGAA
CTTCATGCAG
TTGATCCATG
ATGACTCTCT
CACCTTTAAG
GAGGACATCC
AGAAAGCACA
AGTTTCTGGC
CAGGGGGACA
GTCTCCACGA
GCACATCGCT
AATCTTGCAG
GTAGCCCAGC
TATCAAAAAG
GGAATACTGC
AGACCGTTAA
GGTCGTGGAT
GAACTCGTCA
AAGTAATGGG
AAGGCATAAG
CCCGAGAATA
TCGTTATCGA
GATGGCCCGA
GAGAACCAAA
CTACCCAGAA
GGGACAGAAG
AACAGTAGGG
AAAGGATGAA
GAGGATTGAA
GAGGGTATAA
AAGAACTGGG
GTCCCAAATC
CTTAAGGAAC
ACCCAGTTGA
AAACACCCAG
CTTCAGAATG
AGAAGCTCTA
CCTGTACTAC
CTGCAGAACG
GCAGGGACAT
GTACGTGGAT
CAGGAACTGG
ACATCAATCG
GCTCTCCGAC
TACGACGTGG
ATCATATCGT
GCCCCAGTCT
TTTCTCAAAG
ATGATTCTAT
TGATAATAAA
GTGTTGACAA
GATCCGATAA
AAATAGAGGG
AAGAGTGATA
ACGTCCCCTC
AGAAGAAGTT
GTCAAGAAAA
TGAAAAATTA
TTGGCGGCAG
CTGCTGAACG
CCAAACTGAT
CACACAACGG
AAGTTCGATA
ATCTGACTAA
GGCTGAACGA
GGTGGCCTGT
CTGAGTTGGA
TAAAGCCGGC
TTCATCAAAA
GGCAGCTTGT
TGAGACACGC
CAGATCACCA
AGCACGTGGC
CCAAATTCTC
GATTCACGCA
TGAACACCAA
GTACGATGAA
AATGACAAAC
TGATTCGAGA
GGTGAAAGTT
ATTACTCTGA
AGTCTAAGCT
GGTTTCAGAT
TTCAGAAAGG
ACTTTCAGTT
TTATAAGGTG
AGAGAGATCA
ACAATTACCA
CCATGCGCAT
GATGCCTACC
TGAATGCAGT
GGTAGGCACT
GCACTTATCA
AAAAATATCC
CAAGCTTGAA
TCTGAATTTG
TTTACGGAGA
CTATAAAGTG
TACGATGTTA
GGAAAATGAT
CGCAAAGTCT
GAGCAGGAAA
TAGGCAAGGC
CACCGCTAAG
TACTTCTTTT
ACAGCAATAT
TATGAATTTT
TTCAAGACCG
AGATTACACT
GGCCAATGGA
GAGATTCGGA
AGCGACCACT
TATCGAAACA
AACGGAGAAA
CAGGAGAAAT
CGTGTGGGAC
AAGGGTAGGG
ATTTCGCGAC
AGTCCGGAAG
GTCCTGTCCA
TGCCGCAGGT
GAACATCGTT
AAAAAGACCG
AAGTACAGAC
CGGAGGCTTC
TCCAAGGAAA
GTATCCTCCC
GAAAAGGAAC
AGCGACAAGC
TGATCGCACG
CAAAAAAGAT
TGGGACCCCA
AGAAATACGG
CGGATTCGAT
TCTCCTACAG
TCGCTTACAG
TGTACTGGTT
GTGGCCAAAG
TGGAGAAAGG
GAAGTCTAAA
AAACTCAAAA
GCGTCAAGGA
ACTGCTGGGC
ATCACAATCA
TGGAGCGATC
AAGCTTCGAA
AAAAACCCCA
TCGACTTTCT
CGAGGCGAAA
GGATATAAAG
AGGTCAAAAA
AGACCTCATC
ATTAAGCTTC
CCAAGTACTC
TCTCTTTGAG
CTTGAAAACG
GCCGGAAACG
AATGCTCGCT
AGTGCGGGCG
AGCTGCAGAA
AGGTAACGAG
CTGGCACTGC
CCTCTAAATA
CGTTAATTTC
TTGTATCTGG
CCAGCCACTA
TGAAAAGCTC
AAAGGATCTC
CCGAAGATAA
TGAGCAGAAG
CAGCTGTTCG
TGGAACAACA
CAAACACTAC
CTTGATGAGA
TCATCGAGCA
AATAAGCGAA
TTCTCCAAAA
GAGTGATCCT
CGCCGACGCT
AACCTCGATA
AGGTGCTTTC
TGCTTACAAT
AAGCACAGGG
ATAAGCCCAT
CAGGGAGCAG
GCAGAAAACA
TTATCCACTT
GTTTACTCTG
ACCAACTTGG
GCGCGCCTGC
AGCCTTCAAG
TACTTCGACA
CCACCATAGA
CAGAAAGCGG
TACACCTCTA
CAAAGGAGGT
CCTGGACGCC
ACACTGATTC
ATCAGTCAAT
TACGGGGCTC
TATGAAACAA
GAATCGACCT
CTCTCAGCTC
GGTGGAGACA
GCAGGGCTGA
CCCCAAGAAG
AAGAGGAAGG
TGTGAGCTTG
GAATGGATCT
TCGATCCCGA
TCGTTCAAAC
ATTTGGCAAT
AAAGTTTCTT
AAGATTGAAT
CCTGTTGCCG
GTCTTGCGAC
GATTATCATA
TAATTTCTGT
TGAATTACGT
TAAGCATGTA
ATAATTAACA
TGTAATGCAT
GACGTTATTT
ATGAGATGGG
TTTTTATGAT
TAGAGTCCCG
CAATTATACA
TTTAATACGC
GATAGAAAAC
AAAATATAGC
GCGCAAACTA
GGATAAATTA
TCGCGCGCGG
TGTCATCTAT
GTTACTAGAT
CGGGAATTGC
CAAGCTAATT
CTTGAAGACG
AAAGGGCCTC
GTGATACGCC
TATTTTTATA
GGTTAATGTC
ATGATAATAA
TGGTTTCTTA
GACGTCAGGT
GGCACTTTTC
GGGGAAATGT
GCGCGGAACC
CCTATTTGTT
TATTTTTCTA
AATACATTCA
AATATGTATC
CGCTCATGAG
ACAATAACCC
TGATAAATGC
TTCAATAATG
GGACCGACTC
GCGCTTGAGA
CCGATATCTG
ACGTAAACCT
AAGAGAAAAG
AGCGTTTATT
AGAATAACGG
ATATTTAAAA
GGGCGTGAAA
AGGTTTATCC
GTTCGTCCAT
TTGTATGTGC
ATGCCAACCA
CAGGGTTCCC
CTCGGGATCA
AAGTACTTTG
ATCCAACCCC
TCCGCTGCTA
TAGTGCAGTC
GGCTTCTGAC
GTTCAGTGCA
GCCGTCATCT
GAAAACGACA
TGTCGCACAA
GTCCTAAGTT
ACGCGACAGG
CTGCCGCCCT
GCCCTTTTCC
TGGCGTTTTC
TTGTCGCGTG
TTTTAGTCGC
ATAAAGTAGA
ATACTTGCGA
CTAGAACCGG
AGACATTACG
CCATGAACAA
GAGCGCCGCC
GCTGGCCTGC
TGGGCTATGC
CCGCGTCAGC
ACCGACGACC
AGGACTTGAC
CAACCAACGG
GCCGAACTGC
ACGCGGCCGG
CTGCACCAAG
CTGTTTTCCG
AGAAGATCAC
CGGCACCAGG
CGCGACCGCC
CGGAGCTGGC
CAGGATGCTT
GACCACCTAC
GCCCTGGCGA
CGTTGTGACA
GTGACCAGGC
TAGACCGCCT
GGCCCGCAGC
ACCCGCGACC
TACTGGACAT
TGCCGAGCGC
ATCCAGGAGG
CCGGCGCGGG
CCTGCGTAGC
CTGGCAGAGC
CGTGGGCCGA
CACCACCACG
CCGGCCGGCC
GCATGGTGTT
GACCGTGTTC
GCCGGCATTG
CCGAGTTCGA
GCGTTCCCTA
ATCATCGACC
GCACCCGGAG
CGGGCGCGAG
GCCGCCAAGG
CCCGAGGCGT
GAAGTTTGGC
CCCCGCCCTA
CCCTCACCCC
GGCACAGATC
GCGCACGCCC
GCGAGCTGAT
CGACCAGGAA
GGCCGCACCG
TGAAAGAGGC
GGCTGCACTG
CTTGGCGTGC
AACGCTCGAC
CCTGTACCGC
GCACTTGAGC
GCAGCGAGGA
AGTGACGCCC
ACCGAGGCCA
GGCGGCGCGG
TGCCTTCCGT
GAGGACGCAT
TGACCGAGGC
CGACGCCCTG
GCGGCCGCCG
AGAATGAACG
CCAAGAGGAA
CAAGCATGAA
ACCGCACCAG
GACGGCCAGG
ACGAACCGTT
TTTCATTACC
GAAGAGATCG
AGGCGGAGAT
GATCGCGGCC
GGGTACGTGT
TCGAGCCGCC
CGCGCACCTC
TCAACCGTGC
GGCTGCATGA
AATCCTGGCC
GGTTTGTCTG
ATGCCAAGCT
GGCGGCCTGG
CCGGCCAGCT
TGGCCGCTGA
AGAAACCGAG
CGCCGCCGTC
TAAAAAGGTG
ATGTGTATTT
GAGTAAAACA
GCTTGCGTCA
TGCGGTCGCT
GCGTATATGA
TCCGATGAGT
AAATAAACAA
ATACGCAAGG
GGAACGCATG
AAGGTTATCG
CTGTACTTAA
CCAGAAAGGC
GGGTCAGGCA
AGACGACCAT
CGGAACCCAT
CTAGCCCGCG
CCCTGCAACT
CGCCGGGGCC
GATGTTCTGT
TAGTCGATTC
CGATCCCCAG
GGCAGTGCCC
GCGATTGGGC
GGCCGTGCGG
GAAGATCAAC
CGCTAACCGT
TGTCGGCATC
GACCGCCCGA
CGATTGACCG
CGACGTGAAG
GCCATCGGCC
GGCGCGACTT
CGTAGTGATC
GACGGAGCGC
CCCAGGCGGC
GGACTTGGCT
GTGTCCGCGA
TCAAGGCAGC
CGACTTCGTG
CTGATTCCGG
TGCAGCCAAG
CCCTTACGAC
ATATGGGCCA
CCGCCGACCT
GGTGGAGCTG
GTTAAGCAGC
GCATTGAGGT
CACGGATGGA
AGGCTACAAG
CGGCCTTTGT
CGTGTCGCGG
GCGATCAAAG
GCACGCGCAT
CGGCGGTGAG
GTTGCCGAGG
CGCTGGCCGG
GTACGAGCTG
CCCATTCTTG
AGTCCCGTAT
CACGCAGCGC
GTGAGCTACC
CAGGCACTGC
CGCCGCCGGC
ACAACCGTTC
TTGAATCAGA
ACCCGAGGGC
GACGCTGCCC
GCGAGGTCCA
GGCGCTGGCC
GCTGAAATTA
AATCAAAACT
CATTTGAGTT
AATGAGGTAA
AGAGAAAATG
AGCAAAAGCA
CAAACACGCT
AAGTGCCGGC
CGTCCGAGCG
CACGCAGCAG
CAAGGCTGCA
ACGTTGGCCA
GCCTGGCAGA
CACGCCAGCC
ATGAAGCGGG
TCAACTTTCA
GTTGCCGGCG
GAGGATCACA
CCAAGCTGAA
GATGTACGCG
GTACGCCAAG
GCAAGACCAT
TACCGAGCTG
CTATCTGAAT
AGATCGCGCA
GCTACCAGAG
TAAATGAGCA
AATGAATAAA
TGAGTAGATG
AATTTTAGCG
GCTAAAGGAG
GCGGCATGGA
AAATCAAGAA
CAACCAGGCA
CCGACGCCGT
GGAATGCCCC
ATGTGTGGAG
GAACGGGCGG
TTGGCCAGGC
GTAAGCGGCT
GGGTTGTCTG
CCGGCCCTGC
AATGGCACTG
GAACCCCCAA
GCCCGAGGAA
TCGGCGTGAC
GGTCGCAAAC
CATCCGGCCC
GGTACAAATC
GGCGCGGCGC
TGGGTGATGA
CCTGGTGGAG
AAGTTGAAGG
CCGCGCAGGC
CGCCCAGCGG
CAACGCATCG
AGGCAGAAGC
ACGCCCCGGT
GAATCGTGGC
AAGCGGCCGC
TGATCGAATC
CGCAAAGAAT
CCCGGCAACC
GCCGGCAGCC
GGTGCGCCGT
CGATTAGGAA
GCCGCCCAAG
GGCGACGAGC
AACCAGATTT
TTTCGTTCCG
ATGCTCTATG
ACGTGGGCAC
CCGCGATAGT
CGCAGCATCA
TGGACGTGGC
CGTTTTCCGT
CTGTCGAAGC
GTGACCGACG
AGCTGGCGAG
GTGATCCGCT
ACGAGCTTCC
AGACGGGCAC
GTAGAGGTTT
CCGCAGGGCC
GGCCGGCATG
GCCAGTGTGT
GGGATTACGA
CCTGGTACTG
ATGGCGGTTT
CCCATCTAAC
CGAATCCATG
AACCGATACC
GGGAAGGGAA
GGGAGACAAG
CCCGGCCGCG
TGTTCCGTCC
ACACGTTGCG
GACGTACTCA
AGTTCTGCCG
GCGAGCCGAT
GGCGGAAAGC
AGAAAGACGA
CCTGGTAGAA
ACCTGCATTC
GGTTAAACAC
CACGCACGTT
GCCATGCAGC
GTACGAAGAA
GGCCAAGAAC
GGCCGCCTGG
TGACGGTATC
CGAGGGTGAA
GCCTTGATTA
GCCGCTACAA
GATCGTAAAG
AGCGAAACCG
GGCGGCCGGA
GTACATCGAG
ATCGAGCTAG
CTGATTGGAT
GTACCGCGAG
ATCACAGAAG
GCAAGAACCC
GGACGTGCTG
ACGGTTCACC
CCGATTACTT
TTTGATCGAT
CCCGGCATCG
GCCGTTTTCT
CTACCGCCTG
GCACGCCGCG
CCGCAGGCAA
GGCAGAAGCC
AGATGGTTGT
TCAAGACGAT
CTACGAACGC
AGTGGCAGCG
CCGGAGAGTT
CAAGAAGTTC
TGTTTCACCG
TGCGCAAGCT
GATCGGGTCA
AATGACCTGC
CGGAGTACGA
TTTGAAGGAG
GAGGCGGGGC
AGGCTGGCCC
GATCCTAGTC
ATGCGCTACC
GCAACCTGAT
CGAGGGCGAA
GCATCCGCCG
GTTCCTAATG
TACGGAGCAG
ATGCTAGGGC
AAATTGCCCT
AGCAGGGGAA
AAAGGTCGAA
AAGGACTCTT
TCCTGTGGAT
AGCACGTACA
TTGGGAACCC
AAAGCCGTAC
ATTGGGAACC
GGAACCCGTA
CATTGGGAAC
CCAAAGCCGT
ACATTGGGAA
CCGGTCACAC
ATGTAAGTGA
CTGATATAAA
AGAGAAAAAA
GGCGATTTTT
CCGCCTAAAA
CTCTTTAAAA
CTTATTAAAA
CTCTTAAAAC
CCGCCTGGCC
TGTGCATATC
TGTCTGGCCA
GCGCACAGCC
GAAGAGCTGC
AAAAAGCGCC
TACCCTTCGG
TCGCTGCGCT
CCCTACGCCC
CGCCGCTTCG
CGTCGGCCTA
TCGCGGCCGC
TGGCCGCTCA
AAAATGGCTG
GCCTACGGCC
AGGCAATCTA
CCAGGGCGCG
GACAAGCCGC
GCCGTCGCCA
CTCGACCGCC
GGCGCCCACA
TCAAGGCACC
CTGCCTCGCG
CGTTTCGGTG
ATGACGGTGA
AAACCTCTGA
CACATGCAGC
TCCCGGTGAC
GGTCACAGCT
TGTCTGTAAG
CGGATGCCGG
GAGCAGACAA
GCCCGTCAGG
GCGCGTCAGC
GGGTGTTGGC
GGGTGTCGGG
GCGCAGCCAT
GACCCAGTCA
CGTAGCGATA
GCGGAGTGTA
TACTGGCTTA
ACTATGCGGC
ATCAGAGCAG
ATTGTACTGA
GAGTGCACCA
TATGCGGTGT
GAAATACCGC
ACAGATGCGT
AAGGAGAAAA
TACCGCATCA
GGCGCTCTTC
CGCTTCCTCG
CTCACTGACT
CGCTGCGCTC
GGTCGTTCGG
CTGCGGCGAG
CGGTATCAGC
TCACTCAAAG
GCGGTAATAC
GGTTATCCAC
AGAATCAGGG
GATAACGCAG
GAAAGAACAT
GTGAGCAAAA
GGCCAGCAAA
AGGCCAGGAA
CCGTAAAAAG
GCCGCGTTGC
TGGCGTTTTT
CCATAGGCTC
CGCCCCCCTG
ACGAGCATCA
CAAAAATCGA
CGCTCAAGTC
AGAGGTGGCG
AAACCCGACA
GGACTATAAA
GATACCAGGC
GTTTCCCCCT
GGAAGCTCCC
TCGTGCGCTC
TCCTGTTCCG
ACCCTGCCGC
TTACCGGATA
CCTGTCCGCC
TTTCTCCCTT
CGGGAAGCGT
GGCGCTTTCT
CATAGCTCAC
GCTGTAGGTA
TCTCAGTTCG
GTGTAGGTCG
TTCGCTCCAA
GCTGGGCTGT
GTGCACGAAC
CCCCCGTTCA
GCCCGACCGC
TGCGCCTTAT
CCGGTAACTA
TCGTCTTGAG
TCCAACCCGG
TAAGACACGA
CTTATCGCCA
CTGGCAGCAG
CCACTGGTAA
CAGGATTAGC
AGAGCGAGGT
ATGTAGGCGG
TGCTACAGAG
TTCTTGAAGT
GGTGGCCTAA
CTACGGCTAC
ACTAGAAGGA
CAGTATTTGG
TATCTGCGCT
CTGCTGAAGC
CAGTTACCTT
CGGAAAAAGA
GTTGGTAGCT
CTTGATCCGG
CAAACAAACC
ACCGCTGGTA
GCGGTGGTTT
TTTTGTTTGC
AAGCAGCAGA
TTACGCGCAG
AAAAAAAGGA
TCTCAAGAAG
ATCCTTTGAT
CTTTTCTACG
GGGTCTGACG
CTCAGTGGAA
CGAAAACTCA
CGTTAAGGGA
TTTTGGTCAT
GCATTCTAGG
TGATTATTTG
CCGACTACCT
TGGTGATCTC
GCCTTTCACG
TAGTGGACAA
ATTCTTCCAA
CTGATCTGCG
CGCGAGGCCA
AGCGATCTTC
TTCTTGTCCA
AGATAAGCCT
GTCTAGCTTC
AAGTATGACG
GGCTGATACT
GGGCCGGCAG
GCGCTCCATT
GCCCAGTCGG
CAGCGACATC
CTTCGGCGCG
ATTTTGCCGG
TTACTGCGCT
GTACCAAATG
CGGGACAACG
TAAGCACTAC
ATTTCGCTCA
TCTCCAGCCC
AGTCGGGCGG
CGAGTTCCAT
AGCGTTAAGG
TTTCATTTAG
CGCCTCAAAT
AGATCCTGTT
CAGGAACCGG
ATCAAAGAGT
TCCTCCGCCG
CTGGACCTAC
CAAGGCAACG
CTATGTTCTC
TTGCTTTTGT
CAGCAAGATA
GCCAGATCAA
TGTCGATCGT
GGCTGGCTCG
AAGATACCTG
CAAGAATGTC
ATTGCGCTGC
CATTCTCCAA
ATTGCAGTTC
GCGCTTAGCT
GGATAACGCC
ACGGAATGAT
GTCGTCGTGC
ACAACAATGG
TGACTTCTAC
AGCGCGGAGA
ATCTCGCTCT
CTCCAGGGGA
AGCCGAAGTT
TCCAAAAGGT
CGTTGATCAA
AGCTCGCCGC
GTTGTTTCAT
CAAGCCTTAC
GGTCACCGTA
ACCAGCAAAT
CAATATCACT
GTGTGGCTTC
AGGCCGCCAT
CCACTGCGGA
GCCGTACAAA
TGTACGGCCA
GCAACGTCGG
TTCGAGATGG
CGCTCGATGA
CGCCAACTAC
CTCTGATAGT
TGAGTCGATA
CTTCGGCGAT
CACCGCTTCC
CTCATGATGT
TTAACTTTGT
TTTAGGGCGA
CTGCCCTGCT
GCGTAACATC
GTTGCTGCTC
CATAACATCA
AACATCGACC
CACGGCGTAA
CGCGCTTGCT
GCTTGGATGC
CCGAGGCATA
GACTGTACCC
CAAAAAAACA
GTCATAACAA
GCCATGAAAA
CCGCCACTGC
GCCGTTACCA
CCGCTGCGTT
CGGTCAAGGT
TCTGGACCAG
TTGCGTGAGC
GCATACGCTA
CTTGCATTAC
AGCTTACGAA
CCGAACAGGC
TTATGTCCAC
TGGGTTCGTG
CCTTCATCCG
TTTCCACGGT
GTGCGTCACC
CGGCAACCTT
GGGCAGCAGC
GAAGTCGAGG
CATTTCTGTC
CTGGCTGGAA
CAGAACTTAT
TATTTCCTTC
CTCTTTTCTA
CAGTATTTAA
AGATACCCCA
AGAAGCTAAT
TATAACAAGA
CGAACTCCAA
TTCACTGTTC
CTTGCATTCT
AAAACCTTAA
ATACCAGAAA
ACAGCTTTTT
CAAAGTTGTT
TTCAAAGTTG
GCGTATAACA
TAGTATCGAC
GGAGCCGATT
TTGAAACCGC
GGTGATCACA
GGCAGCAACG
CTCTGTCATC
GTTACAATCA
ACATGCTACC
CTCCGCGAGA
TCATCCGTGT
TTCAAACCCG
GCAGCTTAGT
TGCCGTTCTT
CCGAATAGCA
TCGGTAACAT
GAGCAAAGTC
TGCCGCCTTA
CAACGGCTCT
CCCGCTGACG
CCGTCCCGGA
CTGATGGGCT
GCCTGTATCG
AGTGGTGATT
TTGTGCCGAG
CTGCCGGTCG
GGGAGCTGTT
GGCTGGCTGG
TGGCAGGATA
TATTGTGGTG
TAACGATATC
GGTCTCAGTC
A
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