名称: | pHDE-35S-Cas9-mCherry② |
---|---|
别称: | Plasmid#78931 |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Spe |
筛选标记: | Hyg |
荧光标记: | 红色 |
启动子: | 35S |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTTTACCCGC
CAATATATCC
TGTCAAACAC
TGATAGTTTA
AACTAGTGAA
GTGAAGCTTG
GTCTCAACTA
TGAGACCTAA
GCTGGCACAA
CTATATTTCC
AACATCACTA
GCTACCATCA
AAAGATTGAC
TTCTCATCTT
ACTCGATTGA
AACCAAATTA
ACATAGGGTT
TTTATTTAAA
TAAAAGTTTA
ACCTTCTTTT
TAAAAAATTG
TTCATAGTGT
CATGTCAGAA
CAAGAGCTAC
AAATCACACA
TAGCATGCAT
AAGCGGAGCT
ATGATGAGTG
GTATTGTTTT
GTTCGTCACT
TGTCACTCTT
TTCCAACACA
TAATCCCGAC
AACAACGTAA
GAGCATCTCT
CTCTCTCCAC
ACACACTCAT
GCATGCATGC
ATTCTTACAC
GTGATTGCCA
TGCAAATCTC
CTTTCTCACC
TATAAATACA
AACCAACCCT
TCACTACACT
CTTCACTCAA
ACCAAAACAA
GAAAACATAC
ACAAATAGCA
AAACGGTACC
AACAATGGAT
AACATGGCCA
TCATCAAGGA
GTTCATGCGC
TTCAAGGTGC
ACATGGAGGG
CTCCGTGAAC
GGCCACGAGT
TCGAGATCGA
GGGCGAGGGC
GAGGGCCGCC
CCTACGAGGG
CACCCAGACC
GCCAAGCTGA
AGGTGACCAA
GGGTGGCCCC
CTGCCCTTCG
CCTGGGACAT
CCTGTCCCCT
CAGTTCATGT
ACGGCTCCAA
GGCCTACGTG
AAGCACCCCG
CCGACATCCC
CGACTACTTG
AAGCTGTCCT
TCCCCGAGGG
CTTCAAGTGG
GAGCGCGTGA
TGAACTTCGA
GGACGGCGGC
GTGGTGACCG
TGACCCAGGA
CTCCTCCCTG
CAGGACGGCG
AGTTCATCTA
CAAGGTGAAG
CTGCGCGGCA
CCAACTTCCC
CTCCGACGGC
CCCGTAATGC
AGAAGAAGAC
CATGGGCTGG
GAGGCCTCCT
CCGAGCGGAT
GTACCCCGAG
GACGGCGCCC
TGAAGGGCGA
GATCAAGCAG
AGGCTGAAGC
TGAAGGACGG
CGGCCACTAC
GACGCTGAGG
TCAAGACCAC
CTACAAGGCC
AAGAAGCCCG
TGCAGCTGCC
CGGCGCCTAC
AACGTCAACA
TCAAGTTGGA
CATCACCTCC
CACAACGAGG
ACTACACCAT
CGTGGAACAG
TACGAACGCG
CCGAGGGCCG
CCACTCCACC
GGCGGCATGG
ACGAGCTGTA
CAAGTGAGAG
CTCTTGGACT
CCCATGTTGG
CAAAGGCAAC
CAAACAAACA
ATGAATGATC
CGCTCCTGCA
TATGGGGCGG
TTTGAGTATT
TCAACTGCCA
TTTGGGCTGA
ATTGAAGACA
TGCTCCTGTC
AGAAATTCCG
TGATCTTACT
CAATATTCAG
TAATCTCGGC
CAATATCCTA
AATGTGCGTG
GCTTTATCTG
TCTTTGTATT
GTTTCATCAA
TTCATGTAAC
GTTTGCTTTT
CTTATGAATT
TTCAAATAAA
TTATCGTATT
GAGACCGAAT
TCTGTCTAGA
CCTAACTGAG
TAAGCTAGCT
TGTTCGAGTA
TTATGGCATT
GGGAAAACTG
TTTTTCTTGT
ACCATTTGTT
GTGCTTGTAA
TTTACTGTGT
TTTTTATTCG
GTTTTCGCTA
TCGAACTGTG
AAATGGAAAT
GGATGGAGAA
GAGTTAATGA
ATGATATGGT
CCTTTTGTTC
ATTCTCAAAT
TAATATTATT
TGTTTTTTCT
CTTATTTGTT
GTGTGTTGAA
TTTGAAATTA
TAAGAGATAT
GCAAACATTT
TGTTTTGAGT
AAAAATGTGT
CAAATCGTGG
CCTCTAATGA
CCGAAGTTAA
TATGAGGAGT
AAAACACTAG
ATCCCCAAAC
AAGCTTGAAA
CTGAAGGCGG
GAAACGACAA
TCTGATCATG
AGCGGAGAAT
TAAGGGAGTC
ACGTTATGAC
CCCCGCCGAT
GACGCGGGAC
AAGCCGTTTT
ACGTTTGGAA
CTGACAGAAC
CGCAACGTTG
AAGGAGCCAC
TCAGCCGCGG
GTTTCTGGAG
TTTAATGAGC
TAAGCACATA
CGTCAGAAAC
CATTATTGCG
CGTTCAAAAG
TCGCCTAAGG
TCACTATCAG
CTAGCAAATA
TTTCTTGTCA
AAAATGCTCC
ACTGACGTTC
CATAAATTCC
CCTCGGTATC
CAATTAGAGT
CTCATATTCA
CTCTCAATCC
AAATAATCTG
CACCGGATCC
GCTAGAGGAT
CTCGACCTGC
AAGATCCCGG
GGGGCAATGA
GATATGAAAA
AGCCTGAACT
CACCGCGACG
TCTGTCGAGA
AGTTTCTGAT
CGAAAAGTTC
GACAGCGTCT
CCGACCTGAT
GCAGCTCTCG
GAGGGCGAAG
AATCTCGTGC
TTTCAGCTTC
GATATAGGAG
GGCGTGGATA
TGTCCTGCGG
GTAAATAGCT
GCGCCGATGG
TTTCTACAAA
GATCGTTATG
TTTATCGGCA
CTTTGCATCG
GCCGCGCTCC
CGATTCCGGA
AGTGCTTGAC
ATTGGGGAAT
TCAGCGAGAG
CCTGACCTAT
TGCATCTCCC
GCCGTGCACA
GGGTGTCACG
TTGCAAGACC
TGCCTGAAAC
CGAACTGCCC
GCTGTTCTGC
AGCCGGTCGC
GGAGGCCATG
GATGCGATCG
CTGCGGCCGA
TCTTAGCCAG
ACGAGCGGGT
TCGGCCCATT
CGGACCGCAA
GGAATCGGTC
AATACACTAC
ATGGCGTGAT
TTCATATGCG
CGATTGCTGA
TCCCCATGTG
TATCACTGGC
AAACTGTGAT
GGACGACACC
GTCAGTGCGT
CCGTCGCGCA
GGCTCTCGAT
GAGCTGATGC
TTTGGGCCGA
GGACTGCCCC
GAAGTCCGGC
ACCTCGTGCA
CGCGGATTTC
GGCTCCAACA
ATGTCCTGAC
GGACAATGGC
CGCATAACAG
CGGTCATTGA
CTGGAGCGAG
GCGATGTTCG
GGGATTCCCA
ATACGAGGTC
GCCAACATCT
TCTTCTGGAG
GCCGTGGTTG
GCTTGTATGG
AGCAGCAGAC
GCGCTACTTC
GAGCGGAGGC
ATCCGGAGCT
TGCAGGATCG
CCGCGGCTCC
GGGCGTATAT
GCTCCGCATT
GGTCTTGACC
AACTCTATCA
GAGCTTGGTT
GACGGCAATT
TCGATGATGC
AGCTTGGGCG
CAGGGTCGAT
GCGACGCAAT
CGTCCGATCC
GGAGCCGGGA
CTGTCGGGCG
TACACAAATC
GCCCGCAGAA
GCGCGGCCGT
CTGGACCGAT
GGCTGTGTAG
AAGTACTCGC
CGATAGTGGA
AACCGACGCC
CCAGCACTCG
TCCGGGATCT
TGGAGGTGAT
GTAACATGAT
CACAAGCTGA
TCCCCCGAAT
TTCCCCGATC
GTTCAAACAT
TTGGCAATAA
AGTTTCTTAA
GATTGAATCC
TGTTGCCGGT
CTTGCGATGA
TTATCATATA
ATTTCTGTTG
AATTACGTTA
AGCATGTAAT
AATTAACATG
TAATGCATGA
CGTTATTTAT
GAGATGGGTT
TTTATGATTA
GAGTCCCGCA
ATTATACATT
TAATACGCGA
TAGAAAACAA
AATATAGCGC
GCAAACTAGG
ATAAATTATC
GCGCGCGGTG
TCATCTATGT
TACTAGATCG
GGAATTGATC
CCCCCTCGAC
AGCTTGCATG
CCAGCTTGGG
CTGCAGGTCG
ACGCGTCAAC
ATGGTGGAGC
ACGACACTCT
CGTCTACTCC
AAGAATATCA
AAGATACAGT
CTCAGAAGAC
CAAAGGGCTA
TTGAGACTTT
TCAACAAAGG
GTAATATCGG
GAAACCTCCT
CGGATTCCAT
TGCCCAGCTA
TCTGTCACTT
CATCAAAAGG
ACAGTAGAAA
AGGAAGGTGG
CACCTACAAA
TGCCATCATT
GCGATAAAGG
AAAGGCTATC
GTTCAAGATG
CCTCTGCCGA
CAGTGGTCCC
AAAGATGGAC
CCCCACCCAC
GAGGAGCATC
GTGGAAAAAG
AAGACGTTCC
AACCACGTCT
TCAAAGCAAG
TGGATTGATG
TGATATCTCC
ACTGACGTAA
GGGATGACGC
ACAATCCCAC
TATCCTTCGC
AAGACCCTTC
CTCTATATAA
GGAAGTTCAT
TTCATTTGGA
GAGGACACGA
TATGGACAAG
AAGTACTCCA
TTGGGCTCGA
TATCGGCACA
AACAGCGTCG
GCTGGGCCGT
CATTACGGAC
GAGTACAAGG
TGCCGAGCAA
AAAATTCAAA
GTTCTGGGCA
ATACCGATCG
CCACAGCATA
AAGAAGAACC
TCATTGGCGC
CCTCCTGTTC
GACTCCGGGG
AGACGGCCGA
AGCCACGCGG
CTCAAAAGAA
CAGCACGGCG
CAGATATACC
CGCAGAAAGA
ATCGGATCTG
CTACCTGCAG
GAGATCTTTA
GTAATGAGAT
GGCTAAGGTG
GATGACTCTT
TCTTCCATAG
GCTGGAGGAG
TCCTTTTTGG
TGGAGGAGGA
TAAAAAGCAC
GAGCGCCACC
CAATCTTTGG
CAATATCGTG
GACGAGGTGG
CGTACCATGA
AAAGTACCCA
ACCATATATC
ATCTGAGGAA
GAAGCTTGTA
GACAGTACTG
ATAAGGCTGA
CTTGCGGTTG
ATCTATCTCG
CGCTGGCGCA
TATGATCAAA
TTTCGGGGAC
ACTTCCTCAT
CGAGGGGGAC
CTGAACCCAG
ACAACAGCGA
TGTCGACAAA
CTCTTTATCC
AACTGGTTCA
GACTTACAAT
CAGCTTTTCG
AAGAGAACCC
GATCAACGCA
TCCGGAGTTG
ACGCCAAAGC
AATCCTGAGC
GCTAGGCTGT
CCAAATCCCG
GCGGCTCGAA
AACCTCATCG
CACAGCTCCC
TGGGGAGAAG
AAGAACGGCC
TGTTTGGTAA
TCTTATCGCC
CTGTCACTCG
GGCTGACCCC
CAACTTTAAA
TCTAACTTCG
ACCTGGCCGA
AGATGCCAAG
CTTCAACTGA
GCAAAGACAC
CTACGATGAT
GATCTCGACA
ATCTGCTGGC
CCAGATCGGC
GACCAGTACG
CAGACCTTTT
TTTGGCGGCA
AAGAACCTGT
CAGACGCCAT
TCTGCTGAGT
GATATTCTGC
GAGTGAACAC
GGAGATCACC
AAAGCTCCGC
TGAGCGCTAG
TATGATCAAG
CGCTATGATG
AGCACCACCA
AGACTTGACT
TTGCTGAAGG
CCCTTGTCAG
ACAGCAACTG
CCTGAGAAGT
ACAAGGAAAT
TTTCTTCGAT
CAGTCTAAAA
ATGGCTACGC
CGGATACATT
GACGGCGGAG
CAAGCCAGGA
GGAATTTTAC
AAATTTATTA
AGCCCATCTT
GGAAAAAATG
GACGGCACCG
AGGAGCTGCT
GGTAAAGCTT
AACAGAGAAG
ATCTGTTGCG
CAAACAGCGC
ACTTTCGACA
ATGGAAGCAT
CCCCCACCAG
ATTCACCTGG
GCGAACTGCA
CGCTATCCTC
AGGCGGCAAG
AGGATTTCTA
CCCCTTTTTG
AAAGATAACA
GGGAAAAGAT
TGAGAAAATC
CTCACATTTC
GGATACCCTA
CTATGTAGGC
CCCCTCGCCC
GGGGAAATTC
CAGATTCGCG
TGGATGACTC
GCAAATCAGA
AGAGACCATC
ACTCCCTGGA
ACTTCGAGGA
AGTCGTGGAT
AAGGGGGCCT
CTGCCCAGTC
CTTCATCGAA
AGGATGACTA
ACTTTGATAA
AAATCTGCCT
AACGAAAAGG
TGCTTCCTAA
ACACTCTCTG
CTGTACGAGT
ACTTCACAGT
TTATAACGAG
CTCACCAAGG
TCAAATACGT
CACAGAAGGG
ATGAGAAAGC
CAGCATTCCT
GTCTGGAGAG
CAGAAGAAAG
CTATCGTGGA
CCTCCTCTTC
AAGACGAACC
GGAAAGTTAC
CGTGAAACAG
CTCAAAGAAG
ACTATTTCAA
AAAGATTGAA
TGTTTCGACT
CTGTTGAAAT
CAGCGGAGTG
GAGGATCGCT
TCAACGCATC
CCTGGGAACG
TATCACGATC
TCCTGAAAAT
CATTAAAGAC
AAGGACTTCC
TGGACAATGA
GGAGAACGAG
GACATTCTTG
AGGACATTGT
CCTCACCCTT
ACGTTGTTTG
AAGATAGGGA
GATGATTGAA
GAACGCTTGA
AAACTTACGC
TCATCTCTTC
GACGACAAAG
TCATGAAACA
GCTCAAGAGG
CGCCGATATA
CAGGATGGGG
GCGGCTGTCA
AGAAAACTGA
TCAATGGGAT
CCGAGACAAG
CAGAGTGGAA
AGACAATCCT
GGATTTTCTT
AAGTCCGATG
GATTTGCCAA
CCGGAACTTC
ATGCAGTTGA
TCCATGATGA
CTCTCTCACC
TTTAAGGAGG
ACATCCAGAA
AGCACAAGTT
TCTGGCCAGG
GGGACAGTCT
TCACGAGCAC
ATCGCTAATC
TTGCAGGTAG
CCCAGCTATC
AAAAAGGGAA
TACTGCAGAC
CGTTAAGGTC
GTGGATGAAC
TCGTCAAAGT
AATGGGAAGG
CATAAGCCCG
AGAATATCGT
TATCGAGATG
GCCCGAGAGA
ACCAAACTAC
CCAGAAGGGA
CAGAAGAACA
GTAGGGAAAG
GATGAAGAGG
ATTGAAGAGG
GTATAAAAGA
ACTGGGGTCC
CAAATCCTTA
AGGAACACCC
AGTTGAAAAC
ACCCAGCTTC
AGAATGAGAA
GCTCTACCTG
TACTACCTGC
AGAACGGCAG
GGACATGTAC
GTGGATCAGG
AACTGGACAT
CAATCGGCTC
TCCGACTACG
ACGTGGATCA
TATCGTGCCC
CAGTCTTTTC
TCAAAGATGA
TTCTATTGAT
AATAAAGTGT
TGACAAGATC
CGATAAAAAT
AGAGGGAAGA
GTGATAACGT
CCCCTCAGAA
GAAGTTGTCA
AGAAAATGAA
AAATTATTGG
CGGCAGCTGC
TGAACGCCAA
ACTGATCACA
CAACGGAAGT
TCGATAATCT
GACTAAGGCT
GAACGAGGTG
GCCTGTCTGA
GTTGGATAAA
GCCGGCTTCA
TCAAAAGGCA
GCTTGTTGAG
ACACGCCAGA
TCACCAAGCA
CGTGGCCCAA
ATTCTCGATT
CACGCATGAA
CACCAAGTAC
GATGAAAATG
ACAAACTGAT
TCGAGAGGTG
AAAGTTATTA
CTCTGAAGTC
TAAGCTGGTC
TCAGATTTCA
GAAAGGACTT
TCAGTTTTAT
AAGGTGAGAG
AGATCAACAA
TTACCACCAT
GCGCATGATG
CCTACCTGAA
TGCAGTGGTA
GGCACTGCAC
TTATCAAAAA
ATATCCCAAG
CTTGAATCTG
AATTTGTTTA
CGGAGACTAT
AAAGTGTACG
ATGTTAGGAA
AATGATCGCA
AAGTCTGAGC
AGGAAATAGG
CAAGGCCACC
GCTAAGTACT
TCTTTTACAG
CAATATTATG
AATTTTTTCA
AGACCGAGAT
TACACTGGCC
AATGGAGAGA
TTCGGAAGCG
ACCACTTATC
GAAACAAACG
GAGAAACAGG
AGAAATCGTG
TGGGACAAGG
GTAGGGATTT
CGCGACAGTC
CGGAAGGTCC
TGTCCATGCC
GCAGGTGAAC
ATCGTTAAAA
AGACCGAAGT
ACAGACCGGA
GGCTTCTCCA
AGGAAAGTAT
CCTCCCGAAA
AGGAACAGCG
ACAAGCTGAT
CGCACGCAAA
AAAGATTGGG
ACCCCAAGAA
ATACGGCGGA
TTCGATTCTC
CTACAGTCGC
TTACAGTGTA
CTGGTTGTGG
CCAAAGTGGA
GAAAGGGAAG
TCTAAAAAAC
TCAAAAGCGT
CAAGGAACTG
CTGGGCATCA
CAATCATGGA
GCGATCAAGC
TTCGAAAAAA
ACCCCATCGA
CTTTCTCGAG
GCGAAAGGAT
ATAAAGAGGT
CAAAAAAGAC
CTCATCATTA
AGCTTCCCAA
GTACTCTCTC
TTTGAGCTTG
AAAACGGCCG
GAAACGAATG
CTCGCTAGTG
CGGGCGAGCT
GCAGAAAGGT
AACGAGCTGG
CACTGCCCTC
TAAATACGTT
AATTTCTTGT
ATCTGGCCAG
CCACTATGAA
AAGCTCAAAG
GGTCTCCCGA
AGATAATGAG
CAGAAGCAGC
TGTTCGTGGA
ACAACACAAA
CACTACCTTG
ATGAGATCAT
CGAGCAAATA
AGCGAATTCT
CCAAAAGAGT
GATCCTCGCC
GACGCTAACC
TCGATAAGGT
GCTTTCTGCT
TACAATAAGC
ACAGGGATAA
GCCCATCAGG
GAGCAGGCAG
AAAACATTAT
CCACTTGTTT
ACTCTGACCA
ACTTGGGCGC
GCCTGCAGCC
TTCAAGTACT
TCGACACCAC
CATAGACAGA
AAGCGGTACA
CCTCTACAAA
GGAGGTCCTG
GACGCCACAC
TGATTCATCA
GTCAATTACG
GGGCTCTATG
AAACAAGAAT
CGACCTCTCT
CAGCTCGGTG
GAGACAGCAG
GGCTGACCCC
AAGAAGAAGA
GGAAGGTGTG
ATCGATCCAG
GCCTCCCAGC
TTTCGTCCGT
ATCATCGGTT
TCGACAACGT
TCGTCAAGTT
CAATGCATCA
GTTTCATTGC
CCACACACCA
GAATCCTACT
AAGTTTGAGT
ATTATGGCAT
TGGAAAAGCT
GTTTTCTTCT
ATCATTTGTT
CTGCTTGTAA
TTTACTGTGT
TCTTTCAGTT
TTTGTTTTCG
GACATCAAAA
TGCAAATGGA
TGGATAAGAG
TTAATAAATG
ATATGGTCCT
TTTGTTCATT
CTCAAATTAT
TATTATCTGT
TGTTTTTACT
TTAATGGGTT
GAATTTAAGT
AAGAAAGGAA
CTAACAGTGT
GATATTAAGG
TGCAATGTTA
GACATATAAA
ACAGTCTTTC
ACCTCTCTTT
GGTTATGTCT
TGAATTGGTT
TGTTTCTTCA
CTTATCTGTG
TAATCAAGTT
TACTATGAGT
CTATGATCAA
GTAATTATGC
AATCAAGTTA
AGTACAGTAT
AGGCTTTTTG
TGTCGAGGGG
GTACCGAGTC
GAGGAATTCA
CTGGCCGTCG
TTTTACAACG
TCGTGACTGG
GAAAACCCTG
GCGTTACCCA
ACTTAATCGC
CTTGCAGCAC
ATCCCCCTTT
CGCCAGCTGG
CGTAATAGCG
AAGAGGCCCG
CACCGATCGC
CCTTCCCAAC
AGTTGCGCAG
CCTGAATGGC
GGGTACCGAG
CTCGAATTCA
ATTCGGCGTT
AATTCAGTAC
ATTAAAAACG
TCCGCAATGT
GTTATTAAGT
TGTCTAAGCG
TCAATTTGTT
TACACCACAA
TATATCCTGC
CACCAGCCAG
CCAACAGCTC
CCCGACCGGC
AGCTCGGCAC
AAAATCACCA
CTCGATACAG
GCAGCCCATC
AGTCCGGGAC
GGCGTCAGCG
GGAGAGCCGT
TGTAAGGCGG
CAGACTTTGC
TCATGTTACC
GATGCTATTC
GGAAGAACGG
CAACTAAGCT
GCCGGGTTTG
AAACACGGAT
GATCTCGCGG
AGGGTAGCAT
GTTGATTGTA
ACGATGACAG
AGCGTTGCTG
CCTGTGATCA
ATTCGGGCAC
GAACCCAGTG
GACATAAGCC
TCGTTCGGTT
CGTAAGCTGT
AATGCAAGTA
GCGTAACTGC
CGTCACGCAA
CTGGTCCAGA
ACCTTGACCG
AACGCAGCGG
TGGTAACGGC
GCAGTGGCGG
TTTTCATGGC
TTCTTGTTAT
GACATGTTTT
TTTGGGGTAC
AGTCTATGCC
TCGGGCATCC
AAGCAGCAAG
CGCGTTACGC
CGTGGGTCGA
TGTTTGATGT
TATGGAGCAG
CAACGATGTT
ACGCAGCAGG
GCAGTCGCCC
TAAAACAAAG
TTAAACATCA
TGGGGGAAGC
GGTGATCGCC
GAAGTATCGA
CTCAACTATC
AGAGGTAGTT
GGCGTCATCG
AGCGCCATCT
CGAACCGACG
TTGCTGGCCG
TACATTTGTA
CGGCTCCGCA
GTGGATGGCG
GCCTGAAGCC
ACACAGTGAT
ATTGATTTGC
TGGTTACGGT
GACCGTAAGG
CTTGATGAAA
CAACGCGGCG
AGCTTTGATC
AACGACCTTT
TGGAAACTTC
GGCTTCCCCT
GGAGAGAGCG
AGATTCTCCG
CGCTGTAGAA
GTCACCATTG
TTGTGCACGA
CGACATCATT
CCGTGGCGTT
ATCCAGCTAA
GCGCGAACTG
CAATTTGGAG
AATGGCAGCG
CAATGACATT
CTTGCAGGTA
TCTTCGAGCC
AGCCACGATC
GACATTGATC
TGGCTATCTT
GCTGACAAAA
GCAAGAGAAC
ATAGCGTTGC
CTTGGTAGGT
CCAGCGGCGG
AGGAACTCTT
TGATCCGGTT
CCTGAACAGG
ATCTATTTGA
GGCGCTAAAT
GAAACCTTAA
CGCTATGGAA
CTCGCCGCCC
GACTGGGCTG
GCGATGAGCG
AAATGTAGTG
CTTACGTTGT
CCCGCATTTG
GTACAGCGCA
GTAACCGGCA
AAATCGCGCC
GAAGGATGTC
GCTGCCGACT
GGGCAATGGA
GCGCCTGCCG
GCCCAGTATC
AGCCCGTCAT
ACTTGAAGCT
AGACAGGCTT
ATCTTGGACA
AGAAGAAGAT
CGCTTGGCCT
CGCGCGCAGA
TCAGTTGGAA
GAATTTGTCC
ACTACGTGAA
AGGCGAGATC
ACCAAGGTAG
TCGGCAAATA
ATGTCTAGCT
AGAAATTCGT
TCAAGCCGAC
GCCGCTTCGC
CGGCGTTAAC
TCAAGCGATT
AGATGCACTA
AGCACATAAT
TGCTCACAGC
CAAACTATCA
GGTCAAGTCT
GCTTTTATTA
TTTTTAAGCG
TGCATAATAA
GCCCTACACA
AATTGGGAGA
TATATCATGC
ATGACCAAAA
TCCCTTAACG
TGAGTTTTCG
TTCCACTGAG
CGTCAGACCC
CGTAGAAAAG
ATCAAAGGAT
CTTCTTGAGA
TCCTTTTTTT
CTGCGCGTAA
TCTGCTGCTT
GCAAACAAAA
AAACCACCGC
TACCAGCGGT
GGTTTGTTTG
CCGGATCAAG
AGCTACCAAC
TCTTTTTCCG
AAGGTAACTG
GCTTCAGCAG
AGCGCAGATA
CCAAATACTG
TCCTTCTAGT
GTAGCCGTAG
TTAGGCCACC
ACTTCAAGAA
CTCTGTAGCA
CCGCCTACAT
ACCTCGCTCT
GCTAATCCTG
TTACCAGTGG
CTGCTGCCAG
TGGCGATAAG
TCGTGTCTTA
CCGGGTTGGA
CTCAAGACGA
TAGTTACCGG
ATAAGGCGCA
GCGGTCGGGC
TGAACGGGGG
GTTCGTGCAC
ACAGCCCAGC
TTGGAGCGAA
CGACCTACAC
CGAACTGAGA
TACCTACAGC
GTGAGCTATG
AGAAAGCGCC
ACGCTTCCCG
AAGGGAGAAA
GGCGGACAGG
TATCCGGTAA
GCGGCAGGGT
CGGAACAGGA
GAGCGCACGA
GGGAGCTTCC
AGGGGGAAAC
GCCTGGTATC
TTTATAGTCC
TGTCGGGTTT
CGCCACCTCT
GACTTGAGCG
TCGATTTTTG
TGATGCTCGT
CAGGGGGGCG
GAGCCTATGG
AAAAACGCCA
GCAACGCGGC
CTTTTTACGG
TTCCTGGCCT
TTTGCTGGCC
TTTTGCTCAC
ATGTTCTTTC
CTGCGTTATC
CCCTGATTCT
GTGGATAACC
GTATTACCGC
CTTTGAGTGA
GCTGATACCG
CTCGCCGCAG
CCGAACGACC
GAGCGCAGCG
AGTCAGTGAG
CGAGGAAGCG
GAAGAGCGCC
TGATGCGGTA
TTTTCTCCTT
ACGCATCTGT
GCGGTATTTC
ACACCGCATA
TGGTGCACTC
TCAGTACAAT
CTGCTCTGAT
GCCGCATAGT
TAAGCCAGTA
TACACTCCGC
TATCGCTACG
TGACTGGGTC
ATGGCTGCGC
CCCGACACCC
GCCAACACCC
GCTGACGCGC
CCTGACGGGC
TTGTCTGCTC
CCGGCATCCG
CTTACAGACA
AGCTGTGACC
GTCTCCGGGA
GCTGCATGTG
TCAGAGGTTT
TCACCGTCAT
CACCGAAACG
CGCGAGGCAG
GGTGCCTTGA
TGTGGGCGCC
GGCGGTCGAG
TGGCGACGGC
GCGGCTTGTC
CGCGCCCTGG
TAGATTGCCT
GGCCGTAGGC
CAGCCATTTT
TGAGCGGCCA
GCGGCCGCGA
TAGGCCGACG
CGAAGCGGCG
GGGCGTAGGG
AGCGCAGCGA
CCGAAGGGTA
GGCGCTTTTT
GCAGCTCTTC
GGCTGTGCGC
TGGCCAGACA
GTTATGCACA
GGCCAGGCGG
GTTTTAAGAG
TTTTAATAAG
TTTTAAAGAG
TTTTAGGCGG
AAAAATCGCC
TTTTTTCTCT
TTTATATCAG
TCACTTACAT
GTGTGACCGG
TTCCCAATGT
ACGGCTTTGG
GTTCCCAATG
TACGGGTTCC
GGTTCCCAAT
GTACGGCTTT
GGGTTCCCAA
TGTACGTGCT
ATCCACAGGA
AAGAGACCTT
TTCGACCTTT
TTCCCCTGCT
AGGGCAATTT
GCCCTAGCAT
CTGCTCCGTA
CATTAGGAAC
CGGCGGATGC
TTCGCCCTCG
ATCAGGTTGC
GGTAGCGCAT
GACTAGGATC
GGGCCAGCCT
GCCCCGCCTC
CTCCTTCAAA
TCGTACTCCG
GCAGGTCATT
TGACCCGATC
AGCTTGCGCA
CGGTGAAACA
GAACTTCTTG
AACTCTCCGG
CGCTGCCACT
GCGTTCGTAG
ATCGTCTTGA
ACAACCATCT
GGCTTCTGCC
TTGCCTGCGG
CGCGGCGTGC
CAGGCGGTAG
AGAAAACGGC
CGATGCCGGG
ATCGATCAAA
AAGTAATCGG
GGTGAACCGT
CAGCACGTCC
GGGTTCTTGC
CTTCTGTGAT
CTCGCGGTAC
ATCCAATCAG
CTAGCTCGAT
CTCGATGTAC
TCCGGCCGCC
CGGTTTCGCT
CTTTACGATC
TTGTAGCGGC
TAATCAAGGC
TTCACCCTCG
GATACCGTCA
CCAGGCGGCC
GTTCTTGGCC
TTCTTCGTAC
GCTGCATGGC
AACGTGCGTG
GTGTTTAACC
GAATGCAGGT
TTCTACCAGG
TCGTCTTTCT
GCTTTCCGCC
ATCGGCTCGC
CGGCAGAACT
TGAGTACGTC
CGCAACGTGT
GGACGGAACA
CGCGGCCGGG
CTTGTCTCCC
TTCCCTTCCC
GGTATCGGTT
CATGGATTCG
GTTAGATGGG
AAACCGCCAT
CAGTACCAGG
TCGTAATCCC
ACACACTGGC
CATGCCGGCC
GGCCCTGCGG
AAACCTCTAC
GTGCCCGTCT
GGAAGCTCGT
AGCGGATCAC
CTCGCCAGCT
CGTCGGTCAC
GCTTCGACAG
ACGGAAAACG
GCCACGTCCA
TGATGCTGCG
ACTATCGCGG
GTGCCCACGT
CATAGAGCAT
CGGAACGAAA
AAATCTGGTT
GCTCGTCGCC
CTTGGGCGGC
TTCCTAATCG
ACGGCGCACC
GGCTGCCGGC
GGTTGCCGGG
ATTCTTTGCG
GATTCGATCA
GCGGCCGCTT
GCCACGATTC
ACCGGGGCGT
GCTTCTGCCT
CGATGCGTTG
CCGCTGGGCG
GCCTGCGCGG
CCTTCAACTT
CTCCACCAGG
TCATCACCCA
GCGCCGCGCC
GATTTGTACC
GGGCCGGATG
GTTTGCGACC
GTCACGCCGA
TTCCTCGGGC
TTGGGGGTTC
CAGTGCCATT
GCAGGGCCGG
CAGACAACCC
AGCCGCTTAC
GCCTGGCCAA
CCGCCCGTTC
CTCCACACAT
GGGGCATTCC
ACGGCGTCGG
TGCCTGGTTG
TTCTTGATTT
TCCATGCCGC
CTCCTTTAGC
CGCTAAAATT
CATCTACTCA
TTTATTCATT
TGCTCATTTA
CTCTGGTAGC
TGCGCGATGT
ATTCAGATAG
CAGCTCGGTA
ATGGTCTTGC
CTTGGCGTAC
CGCGTACATC
TTCAGCTTGG
TGTGATCCTC
CGCCGGCAAC
TGAAAGTTGA
CCCGCTTCAT
GGCTGGCGTG
TCTGCCAGGC
TGGCCAACGT
TGCAGCCTTG
CTGCTGCGTG
CGCTCGGACG
GCCGGCACTT
AGCGTGTTTG
TGCTTTTGCT
CATTTTCTCT
TTACCTCATT
AACTCAAATG
AGTTTTGATT
TAATTTCAGC
GGCCAGCGCC
TGGACCTCGC
GGGCAGCGTC
GCCCTCGGGT
TCTGATTCAA
GAACGGTTGT
GCCGGCGGCG
GCAGTGCCTG
GGTAGCTCAC
GCGCTGCGTG
ATACGGGACT
CAAGAATGGG
CAGCTCGTAC
CCGGCCAGCG
CCTCGGCAAC
CTCACCGCCG
ATGCGCGTGC
CTTTGATCGC
CCGCGACACG
ACAAAGGCCG
CTTGTAGCCT
TCCATCCGTG
ACCTCAATGC
GCTGCTTAAC
CAGCTCCACC
AGGTCGGCGG
TGGCCCATAT
GTCGTAAGGG
CTTGGCTGCA
CCGGAATCAG
CACGAAGTCG
GCTGCCTTGA
TCGCGGACAC
AGCCAAGTCC
GCCGCCTGGG
GCGCTCCGTC
GATCACTACG
AAGTCGCGCC
GGCCGATGGC
CTTCACGTCG
CGGTCAATCG
TCGGGCGGTC
GATGCCGACA
ACGGTTAGCG
GTTGATCTTC
CCGCACGGCC
GCCCAATCGC
GGGCACTGCC
CTGGGGATCG
GAATCGACTA
ACAGAACATC
GGCCCCGGCG
AGTTGCAGGG
CGCGGGCTAG
ATGGGTTGCG
ATGGTCGTCT
TGCCTGACCC
GCCTTTCTGG
TTAAGTACAG
CGATAACCTT
CATGCGTTCC
CCTTGCGTAT
TTGTTTATTT
ACTCATCGCA
TCATATACGC
AGCGACCGCA
TGACGCAAGC
TGTTTTACTC
AAATACACAT
CACCTTTTTA
GACGGCGGCG
CTCGGTTTCT
TCAGCGGCCA
AGCTGGCCGG
CCAGGCCGCC
AGCTTGGCAT
CAGACAAACC
GGCCAGGATT
TCATGCAGCC
GCACGGTTGA
GACGTGCGCG
GGCGGCTCGA
ACACGTACCC
GGCCGCGATC
ATCTCCGCCT
CGATCTCTTC
GGTAATGAAA
AACGGTTCGT
CCTGGCCGTC
CTGGTGCGGT
TTCATGCTTG
TTCCTCTTGG
CGTTCATTCT
CGGCGGCCGC
CAGGGCGTCG
GCCTCGGTCA
ATGCGTCCTC
ACGGAAGGCA
CCGCGCCGCC
TGGCCTCGGT
GGGCGTCACT
TCCTCGCTGC
GCTCAAGTGC
GCGGTACAGG
GTCGAGCGAT
GCACGCCAAG
CAGTGCAGCC
GCCTCTTTCA
CGGTGCGGCC
TTCCTGGTCG
ATCAGCTCGC
GGGCGTGCGC
GATCTGTGCC
GGGGTGAGGG
TAGGGCGGGG
GCCAAACTTC
ACGCCTCGGG
CCTTGGCGGC
CTCGCGCCCG
CTCCGGGTGC
GGTCGATGAT
TAGGGAACGC
TCGAACTCGG
CAATGCCGGC
GAACACGGTC
AACACCATGC
GGCCGGCCGG
CGTGGTGGTG
TCGGCCCACG
GCTCTGCCAG
GCTACGCAGG
CCCGCGCCGG
CCTCCTGGAT
GCGCTCGGCA
ATGTCCAGTA
GGTCGCGGGT
GCTGCGGGCC
AGGCGGTCTA
GCCTGGTCAC
TGTCACAACG
TCGCCAGGGC
GTAGGTGGTC
AAGCATCCTG
GCCAGCTCCG
GGCGGTCGCG
CCTGGTGCCG
GTGATCTTCT
CGGAAAACAG
CTTGGTGCAG
CCGGCCGCGT
GCAGTTCGGC
CCGTTGGTTG
GTCAAGTCCT
GGTCGTCGGT
GCTGACGCGG
GCATAGCCCA
GCAGGCCAGC
GGCGGCGCTC
TTGTTCATGG
CGTAATGTCT
CCGGTTCTAG
TCGCAAGTAT
TCTACTTTAT
GCGACTAAAA
CACGCGACAA
GAAAACGCCA
GGAAAAGGGC
AGGGCGGCAG
CCTGTCGCGT
AACTTAGGAC
TTGTGCGACA
TGTCGTTTTC
AGAAGACGGC
TGCACTGAAC
GTCAGAAGCC
GACTGCACTA
TAGCAGCGGA
GGGGTTGGAT
CAAAGTACTT
TAAAGTACTT
TAAAGTACTT
TAAAGTACTT
TGATCCCGAG
GGGAACCCTG
TGGTTGGCAT
GCACATACAA
ATGGACGAAC
GGATAAACCT
TTTCACGCCC
TTTTAAATAT
CCGATTATTC
TAATAAACGC
TCTTTTCTCT
TAG