名称: | pTC217 |
---|---|
别称: | Plasmid:#70018 |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Kan |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | 35S |
复制子: | pVS1 |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGGCAGGATA
TATTGTGGTG
TAAACAAATT
GACGCTTAGA
CAACTTAATA
ACACATTGCG
GACGTTTTTA
ATGTAGAGCT
CAAAGTTTAA
CGCGTTAGCA
GAAGGCATGT
TGTTGTGACT
CCGAGGGGTT
GCCTCAAACT
CTATCTTATA
ACCGGCGTGG
AGGCATGGAG
GCAGGGGTAT
TTTGGTCATT
TTAATAGATA
GTGGAAAATG
ACGTGGAATT
TACTTAAAGA
CGAAGTCTTT
GCGACAAGGG
GGGGCCCACG
CCGAATTTAA
TATTACCGGC
GTGGCCCCCC
CTTATCGCGA
GTGCTTTAGC
ACGAGCGGTC
CAGATTTAAA
GTAGAAAATT
TCCCGCCCAC
TAGGGTTAAA
GGTGTTCACA
CTATAAAAGC
ATATACGATG
TGATGGTATT
TGATGGAGCG
TATATTGTAT
CAGGTATTTC
CGTTGGATAC
GAATTATTCG
TACGACCCTC
GGTACCGATC
GGCGCGCCAG
ATTTGCCTTT
TCAATTTCAG
AAAGAATGCT
AACCCACAGA
TGGTTAGAGA
GGCTTACGCA
GCAGGTATCA
TCAAGACGAT
CTACCCGAGC
AATAATCTCC
AGGAAATCAA
ATACCTTCCC
AAGAAGGTTA
AAGATGCAGT
CAAAAGATTC
AGGACTAACT
GCATCAAGAA
CACAGAGAAA
GATATATTTC
TCAAGATCAG
AAGTACTATT
CCAGTATGGA
CGATTCAAGG
CTTGCTTCAC
AAACCAAGGC
AAGTAATAGA
GATTGGAGTC
TCTAAAAAGG
TAGTTCCCAC
TGAATCAAAG
GCCATGGAGT
CAAAGATTCA
AATAGAGGAC
CTAACAGAAC
TCGCCGTAAA
GACTGGCGAA
CAGTTCATAC
AGAGTCTCTT
ACGACTCAAT
GACAAGAAGA
AAATCTTCGT
CAACATGGTG
GAGCACGACA
CACTTGTCTA
CTCCAAAAAT
ATCAAAGATA
CAGTCTCAGA
AGACCAAAGG
GCAATTGAGA
CTTTTCAACA
AAGGGTAATA
TCCGGAAACC
TCCTCGGATT
CCATTGCCCA
GCTATCTGTC
ACTTTATTGT
GAAGATAGTG
GAAAAGGAAG
GTGGCTCCTA
CAAATGCCAT
CATTGCGATA
AAGGAAAGGC
CATCGTTGAA
GATGCCTCTG
CCGACAGTGG
TCCCAAAGAT
GGACCCCCAC
CCACGAGGAG
CATCGTGGAA
AAAGAAGACG
TTCCAACCAC
GTCTTCAAAG
CAAGTGGATT
GATGTGATAT
CTCCACTGAC
GTAAGGGATG
ACGCACAATC
CCACTATCCT
TCGCAAGACC
CTTCCTCTAT
ATAAGGAAGT
TCATTTCATT
TGGAGAGAAC
ACGGGGGACT
CCTGCAGGTA
GATCGCTCGT
CGACATGGAT
AAGAAGTACT
CTATCGGACT
CGATATCGGA
ACTAACTCTG
TGGGATGGGC
TGTGATCACC
GATGAGTACA
AGGTGCCATC
TAAGAAGTTC
AAGGTTCTCG
GAAACACCGA
TAGGCACTCT
ATCAAGAAAA
ACCTTATCGG
TGCTCTCCTC
TTCGATTCTG
GTGAAACTGC
TGAGGCTACC
AGACTCAAGA
GAACCGCTAG
AAGAAGGTAC
ACCAGAAGAA
AGAACAGGAT
CTGCTACCTC
CAAGAGATCT
TCTCTAACGA
GATGGCTAAA
GTGGATGATT
CATTCTTCCA
CAGGCTCGAA
GAGTCATTCC
TCGTGGAAGA
AGATAAGAAG
CACGAGAGGC
ACCCTATCTT
CGGAAACATC
GTTGATGAGG
TGGCATACCA
CGAGAAGTAC
CCTACTATCT
ACCACCTCAG
AAAGAAGCTC
GTTGATTCTA
CTGATAAGGC
TGATCTCAGG
CTCATCTACC
TCGCTCTCGC
TCACATGATC
AAGTTCAGAG
GACACTTCCT
CATCGAGGGT
GATCTCAACC
CTGATAACTC
TGATGTGGAT
AAGTTGTTCA
TCCAGCTCGT
GCAGACCTAC
AACCAGCTTT
TCGAAGAGAA
CCCTATCAAC
GCTTCAGGTG
TGGATGCTAA
GGCTATCCTC
TCTGCTAGGC
TCTCTAAGTC
AAGAAGGCTT
GAGAACCTCA
TTGCTCAGCT
CCCTGGTGAG
AAGAAGAACG
GACTTTTCGG
AAACTTGATC
GCTCTCTCTC
TCGGACTCAC
CCCTAACTTC
AAGTCTAACT
TCGATCTCGC
TGAGGATGCA
AAGCTCCAGC
TCTCAAAGGA
TACCTACGAT
GATGATCTCG
ATAACCTCCT
CGCTCAGATC
GGAGATCAGT
ACGCTGATTT
GTTCCTCGCT
GCTAAGAACC
TCTCTGATGC
TATCCTCCTC
AGTGATATCC
TCAGAGTGAA
CACCGAGATC
ACCAAGGCTC
CACTCTCAGC
TTCTATGATC
AAGAGATACG
ATGAGCACCA
CCAGGATCTC
ACACTTCTCA
AGGCTCTTGT
TAGACAGCAG
CTCCCAGAGA
AGTACAAAGA
GATTTTCTTC
GATCAGTCTA
AGAACGGATA
CGCTGGTTAC
ATCGATGGTG
GTGCATCTCA
AGAAGAGTTC
TACAAGTTCA
TCAAGCCTAT
CCTCGAGAAG
ATGGATGGAA
CCGAGGAACT
CCTCGTGAAG
CTCAATAGAG
AGGATCTTCT
CAGAAAGCAG
AGGACCTTCG
ATAACGGATC
TATCCCTCAT
CAGATCCACC
TCGGAGAGTT
GCACGCTATC
CTTAGAAGGC
AAGAGGATTT
CTACCCATTC
CTCAAGGATA
ACAGGGAAAA
GATTGAGAAG
ATTCTCACCT
TCAGAATCCC
TTACTACGTG
GGACCTCTCG
CTAGAGGAAA
CTCAAGATTC
GCTTGGATGA
CCAGAAAGTC
TGAGGAAACC
ATCACCCCTT
GGAACTTCGA
AGAGGTGGTG
GATAAGGGTG
CTAGTGCTCA
GTCTTTCATC
GAGAGGATGA
CCAACTTCGA
TAAGAACCTT
CCAAACGAGA
AGGTGCTCCC
TAAGCACTCT
TTGCTCTACG
AGTACTTCAC
CGTGTACAAC
GAGTTGACCA
AGGTTAAGTA
CGTGACCGAG
GGAATGAGGA
AGCCTGCTTT
TTTGTCAGGT
GAGCAAAAGA
AGGCTATCGT
TGATCTCTTG
TTCAAGACCA
ACAGAAAGGT
GACCGTGAAG
CAGCTCAAAG
AGGATTACTT
CAAGAAAATC
GAGTGCTTCG
ATTCAGTTGA
GATTTCTGGT
GTTGAGGATA
GGTTCAACGC
ATCTCTCGGA
ACCTACCACG
ATCTCCTCAA
GATCATTAAG
GATAAGGATT
TCTTGGATAA
CGAGGAAAAC
GAGGATATCT
TGGAGGATAT
CGTTCTTACC
CTCACCCTCT
TTGAAGATAG
AGAGATGATT
GAAGAAAGGC
TCAAGACCTA
CGCTCATCTC
TTCGATGATA
AGGTGATGAA
GCAGTTGAAG
AGAAGAAGAT
ACACTGGTTG
GGGAAGGCTC
TCAAGAAAGC
TCATTAACGG
AATCAGGGAT
AAGCAGTCTG
GAAAGACAAT
CCTTGATTTC
CTCAAGTCTG
ATGGATTCGC
TAACAGAAAC
TTCATGCAGC
TCATCCACGA
TGATTCTCTC
ACCTTTAAAG
AGGATATCCA
GAAGGCTCAG
GTTTCAGGAC
AGGGTGATAG
TCTCCATGAG
CATATCGCTA
ACCTCGCTGG
ATCTCCTGCA
ATCAAGAAGG
GAATCCTCCA
GACTGTGAAG
GTTGTGGATG
AGTTGGTGAA
GGTGATGGGA
AGGCATAAGC
CTGAGAACAT
CGTGATCGAA
ATGGCTAGAG
AGAACCAGAC
CACTCAGAAG
GGACAGAAGA
ACTCTAGGGA
AAGGATGAAG
AGGATCGAGG
AAGGTATCAA
AGAGCTTGGA
TCTCAGATCC
TCAAAGAGCA
CCCTGTTGAG
AACACTCAGC
TCCAGAATGA
GAAGCTCTAC
CTCTACTACC
TCCAGAACGG
AAGGGATATG
TATGTGGATC
AAGAGTTGGA
TATCAACAGG
CTCTCTGATT
ACGATGTTGA
TCATATCGTG
CCACAGTCAT
TCTTGAAGGA
TGATTCTATC
GATAACAAGG
TGCTCACCAG
GTCTGATAAG
AACAGGGGTA
AGAGTGATAA
CGTGCCAAGT
GAAGAGGTTG
TGAAGAAAAT
GAAGAACTAT
TGGAGGCAGC
TCCTCAACGC
TAAGCTCATC
ACTCAGAGAA
AGTTCGATAA
CTTGACTAAG
GCTGAGAGGG
GAGGACTCTC
TGAATTGGAT
AAGGCAGGAT
TCATCAAGAG
GCAGCTTGTG
GAAACCAGGC
AGATCACTAA
GCACGTTGCA
CAGATCCTCG
ATTCTAGGAT
GAACACCAAG
TACGATGAGA
ACGATAAGTT
GATCAGGGAA
GTGAAGGTTA
TCACCCTCAA
GTCAAAGCTC
GTGTCTGATT
TCAGAAAGGA
TTTCCAATTC
TACAAGGTGA
GGGAAATCAA
CAACTACCAC
CACGCTCACG
ATGCTTACCT
TAACGCTGTT
GTTGGAACCG
CTCTCATCAA
GAAGTATCCT
AAGCTCGAGT
CAGAGTTCGT
GTACGGTGAT
TACAAGGTGT
ACGATGTGAG
GAAGATGATC
GCTAAGTCTG
AGCAAGAGAT
CGGAAAGGCT
ACCGCTAAGT
ATTTCTTCTA
CTCTAACATC
ATGAATTTCT
TCAAGACCGA
GATTACCCTC
GCTAACGGTG
AGATCAGAAA
GAGGCCACTC
ATCGAGACAA
ACGGTGAAAC
AGGTGAGATC
GTGTGGGATA
AGGGAAGGGA
TTTCGCTACC
GTTAGAAAGG
TGCTCTCTAT
GCCACAGGTG
AACATCGTTA
AGAAAACCGA
GGTGCAGACC
GGTGGATTCT
CTAAAGAGTC
TATCCTCCCT
AAGAGGAACT
CTGATAAGCT
CATTGCTAGG
AAGAAGGATT
GGGACCCTAA
GAAATACGGT
GGTTTCGATT
CTCCTACCGT
GGCTTACTCT
GTTCTCGTTG
TGGCTAAGGT
TGAGAAGGGA
AAGAGTAAGA
AGCTCAAGTC
TGTTAAGGAA
CTTCTCGGAA
TCACTATCAT
GGAAAGGTCA
TCTTTCGAGA
AGAACCCAAT
CGATTTCCTC
GAGGCTAAGG
GATACAAAGA
GGTTAAGAAG
GATCTCATCA
TCAAGCTCCC
AAAGTACTCA
CTCTTCGAAC
TCGAGAACGG
TAGAAAGAGG
ATGCTCGCTT
CTGCTGGTGA
GCTTCAAAAG
GGAAACGAGC
TTGCTCTCCC
ATCTAAGTAC
GTTAACTTTC
TTTACCTCGC
TTCTCACTAC
GAGAAGTTGA
AGGGATCTCC
AGAAGATAAC
GAGCAGAAGC
AACTTTTCGT
TGAGCAGCAC
AAGCACTACT
TGGATGAGAT
CATCGAGCAG
ATCTCTGAGT
TCTCTAAAAG
GGTGATCCTC
GCTGATGCAA
ACCTCGATAA
GGTGTTGTCT
GCTTACAACA
AGCACAGAGA
TAAGCCTATC
AGGGAACAGG
CAGAGAACAT
CATCCATCTC
TTCACCCTTA
CCAACCTCGG
TGCTCCTGCT
GCTTTCAAGT
ACTTCGATAC
AACCATCGAT
AGGAAGAGAT
ACACCTCTAC
CAAAGAAGTG
CTCGATGCTA
CCCTCATCCA
TCAGTCTATC
ACTGGACTCT
ACGAGACTAG
GATCGATCTC
TCACAGCTCG
GTGGTGATTC
AAGGGCTGAT
CCTAAGAAGA
AGAGGAAGGT
TTGACTCGAG
ATATGAAGAT
GAAGATGAAA
TATTTGGTGT
GTCAAATAAA
AAGCTTGTGT
GCTTAAGTTT
GTGTTTTTTT
CTTGGCTTGT
TGTGTTATGA
ATTTGTGGCT
TTTTCTAATA
TTAAATGAAT
GTAAGATCAC
ATTATAATGA
ATAAACAAAT
GTTTCTATAA
TCCATTGTGA
ATGTTTTGTT
GGATCTCTTC
TGCAGCATAT
AACTACTGTA
TGTGCTATGG
TATGGACTAT
GGAATATGAT
TAAAGATAAG
GAGCTCCGGT
GACGGACCCA
TGGCTTCGTT
GAACAACGGA
AACTCGACTT
GCCTTCCGCA
CAATACATCA
TTTCTTCTTA
GCTTTTTTTC
TTCTTCTTCG
TTCATACAGT
TTTTTTTTGT
TTATCAGCTT
ACATTTTCTT
GAACCGTAGC
TTTCGTTTTC
TTCTTTTTAA
CTTTCCATTC
GGAGTTTTTG
TATCTTGTTT
CATAGTTTGT
CCCAGGATTA
GAATGATTAG
GCATCGAACC
TTCAAGAATT
TGATTGAATA
AAACATCTTC
ATTCTTAAGA
TATGAAGATA
ATCTTCAAAA
GGCCCCTGGG
AATCTGAAAG
AAGAGAAGCA
GGCCCATTTA
TATGGGAAAG
AACAATAGTA
TTTCTTATAT
AGGCCCATTT
AAGTTGAAAA
CAATCTTCAA
AAGTCCCACA
TCGCTTAGAT
AAGAAAACGA
AGCTGAGTTT
ATATACAGCT
AGAGTCGAAG
TAGTGATTGA
CAATTTAATA
CACCTTTTGT
TTTAGAGCTA
GAAATAGCAA
GTTAAAATAA
GGCTAGTCCG
TTATCAACTT
GAAAAAGTGG
CACCGAGTCG
GTGCTTTTTT
TCCCGGCGAG
CTTTGGGTAC
GTCACGTGGC
TCGAGCGCGT
AGTCCTCGGT
AACCACAACA
CTTGTCGGTG
AGATTATTTA
ATGCTGATTA
GATTAGACAA
AAATTAATTA
GTTTTGAGTA
GTGGCGTAAG
TGTAAATAAT
TAGTCTCTTT
TTTAACTTAG
AAAATAGTTT
AATCCTTAGT
ATAAATAGTC
AAAATCACTG
GAATGAAAAA
CAGTTTTTAA
TTTTTCCAAA
TTTGATTCTG
ATACCATGTT
AAATTCGTGG
TTCAAAATCA
CTGCAATGAA
AAGAGCAATA
TTGTTTAACT
TTTTTTAGGA
AAATCGAATT
GATTTATAGT
CAGTTGATAT
AGAGTGAATA
CATAAGGAAC
ATATACAGTT
GATACAATTG
TATAATTCGT
TCATACACTT
AATACAAAGT
GAACCCACAA
GGAACATATA
CACTTAATAT
AATTGTATTC
CTTGATACAA
ACCAATTTTG
TTCGTGTCTC
TACTCTCTAT
TTCAATTTCG
CTTGACTCTT
TACTTTTTCT
AATATGTAGC
TATAAATCGT
AATTAAACAA
TACTATATCT
CTAAATCTCT
TATTAAGCTC
AAACTATGGT
CATATTCGAA
AAAATCCTTT
TAAATATTGG
TCCCCTCTCA
CGATTAATGA
TAGTTATAAC
TAACATTCAA
ATTTTAGTTG
TACTTGACAT
CTAAAACTTA
AAAAATAGTA
CAAGTTAACT
TTTTCTTTTT
TTTAAAAAAA
GGAAATACTT
GTATTTATTT
TTTTAATATA
TAGTTATATT
TTTGGTTATT
TGAAAATACT
TGATCTGTCA
TGTATGCTCA
GTTAAATATC
GTCACATTAT
AGAGAAAAAA
GTAATAGGAG
AAAAAAATTA
AAAATTATTT
CGAAAAATCA
AAATTTTTTT
TTGATTGAAA
TGAAAGATGG
GTTTCCCAAT
CGAGGCTGGC
AGGATAGGTA
CATTGGGAAA
TTTGGATTTG
TGTGTTGAAA
ATGATTGTTC
AATTTGGCTT
TTATAACATT
TGTCGTTTAT
AATTTGTAGA
AGGCTCTCTA
CAAGTTGGAC
TGAAGGCGGG
AAACGACAAT
CTGATCATGA
GCGGAGAATT
AAGGGAGTCA
CGTTATGACC
CCCGCCGATG
ACGCGGGACA
AGCCGTTTTA
CGTTTGGAAC
TGACAGAACC
GCAACGTTGA
AGGAGCCACT
CAGCCGCGGG
TTTCTGGAGT
TTAATGAGCT
AAGCACATAC
GTCAGAAACC
ATTATTGCGC
GTTCAAAAGT
CGCCTAAGGT
CACTATCAGC
TAGCAAATAT
TTCTTGTCAA
AAATGCTCCA
CTGACGTTCC
ATAAATTCCC
CTCGGTATCC
AATTAGAGTC
TCATATTCAC
TCTCAATCCA
AATAATCTGC
ACCGGATCCG
CTAGAGGATC
TCGACCTGCA
AGATCCCGGG
GGGCAATGAG
ATCCTAGGAT
GGGGATTGAA
CAAGATGGAT
TGCACGCAGG
TTCTCCGGCC
GCTTGGGTGG
AGAGGCTATT
CGGCTATGAC
TGGGCACAAC
AGACAATCGG
CTGCTCTGAT
GCCGCCGTGT
TCCGGCTGTC
AGCGCAGGGG
CGCCCGGTTC
TTTTTGTCAA
GACCGACCTG
TCCGGTGCCC
TGAATGAACT
CCAGGACGAG
GCAGCGCGGC
TATCGTGGCT
GGCCACGACG
GGCGTTCCTT
GCGCAGCTGT
GCTCGACGTT
GTCACTGAAG
CGGGAAGGGA
CTGGCTGCTA
TTGGGCGAAG
TGCCGGGGCA
GGATCTCCTG
TCATCTCACC
TTGCTCCTGC
CGAGAAAGTA
TCCATCATGG
CTGATGCAAT
GCGGCGGCTG
CATACGCTTG
ATCCGGCTAC
CTGCCCATTC
GACCACCAAG
CGAAACATCG
CATCGAGCGA
GCACGTACTC
GGATGGAAGC
CGGTCTTGTC
GATCAGGATG
ATCTGGACGA
AGAGCATCAG
GGGCTCGCGC
CAGCCGAACT
GTTCGCCAGG
CTCAAGGCGC
GCATGCCCGA
CGGCGAGGAT
CTCGTCGTGA
CACATGGCGA
TGCCTGCTTG
CCGAATATCA
TGGTGGAAAA
TGGCCGCTTT
TCTGGATTCA
TCGACTGTGG
CCGGCTGGGT
GTGGCGGACC
GCTATCAGGA
CATAGCGTTG
GCTACCCGTG
ATATTGCTGA
AGAGCTTGGC
GGCGAATGGG
CTGACCGCTT
CCTCGTGCTT
TACGGTATCG
CCGCTCCCGA
TTCGCAGCGC
ATCGCCTTCT
ATCGCCTTCT
TGACGAGTTC
TTCTGATTAA
TTAACGATCG
ACAAGCTCTA
TTTTCTCCAT
AATAATGTGT
GAGTAGTTCC
CAGATAAGGG
AATTAGGGTT
CCTATAGGGT
TTCGCTCATG
TGTTGAGCAT
ATAAGAAACC
CTTAGTATGT
ATTTGTATTT
GTAAAATACT
TCTATCAATA
AAATTTCTAA
TTCCTAAAAC
CAAAATCCAG
TACTAAAATC
CAGATCCCCC
GAATTAATTC
GGCGTTAATT
CAGCATGGAG
TCAAAGATTC
AAATAGAGGA
CCTAACAGAA
CTCGCCGTAA
AGACTGGCGA
ACAGTTCATA
CAGAGTCTCT
TACGACTCAA
TGACAAGAAG
AAAATCTTCG
TCAACATGGT
GGAGCACGAC
ACACTTGTCT
ACTCCAAAAA
TATCAAAGAT
ACAGTCTCAG
AAGACCAAAG
GGCAATTGAG
ACTTTTCAAC
AAAGGGTAAT
ATCCGGAAAC
CTCCTCGGAT
TCCATTGCCC
AGCTATCTGT
CACTTTATTG
TGAAGATAGT
GGAAAAGGAA
GGTGGCTCCT
ACAAATGCCA
TCATTGCGAT
AAAGGAAAGG
CCATCGTTGA
AGATGCCTCT
GCCGACAGTG
GTCCCAAAGA
TGGACCCCCA
CCCACGAGGA
GCATCGTGGA
AAAAGAAGAC
GTTCCAACCA
CGTCTTCAAA
GCAAGTGGAT
TGATGTGATA
TCTCCACTGA
CGTAAGGGAT
GACGCACAAT
CCCACTATCC
TTCGCAAGAC
CCTTCCTCTA
TATAAGGAAG
TTCATTTCAT
TTGGAGAGAA
CACGGGGGAC
TCTTGACAGT
AGTATAATAT
ATTATCAAAT
TATTATGAAC
AGTACATCTA
TGTCTTCATT
GGGAGTGAGA
AAAGGTTCAT
GGACTGATGA
AGAAGATTTT
CTTCTAAGAA
AATGTATTGA
TAAGTATGGT
GAAGGAAAAT
GGCATCTTGT
TCCCATAAGA
GCTGGTAACT
ATTAAATTAA
CTATCACGTT
ATTTTTATTT
GTCTTTCTGT
CTCATTTTAT
TTGACGTTAT
TACGAATATC
ATCTGAAAAT
GTACGTGCAG
GTCTGAATAG
ATGTCGGAAA
AGTTGTAGAT
TGAGGTGGCT
GAATTATCTA
AGGCCACATA
TCAAGAGAGG
TGACTTTGAA
CAAGATGAAG
TGGATCTCAT
TTTGAGGCTT
CATAAGCTCT
TAGGCAACAG
GCATGCAAGT
TTATGTTTTG
ACAAAATTTG
ATTAGTATAT
ATTATATATA
CGTGTGACTA
TTTCATCTAA
ATGTTACGTT
ATTTTACGTA
GATGGTCACT
TATTGCTGGT
AGACTTCCCG
GAAGGACAGC
TAACGATGTG
AAAAACTATT
GGAACACTAA
TCTTCTAAGG
AAGTTAAATA
CTACTAAAAT
TGTTCCTCGC
GAAAAGATTA
ACAATAAGTG
TGGAGAAATT
AGTACTAAGA
TTGAAATTAT
AAAACCTCAA
CGACGCAAGT
ATTTCTCAAG
CACAATGAAG
AATGTTACAA
ACAATAATGT
AATTTTGGAC
GAGGAGGAAC
ATTGCATTTA
AATGAGTGTA
CTTCAAGTCA
CACCGGCGAG
TGTTTGATCG
CCGGCGGTAC
CGAGTGTACT
TCAAGTCAGT
GGGAAATCAA
TAAAATGATT
ATTTTATGAA
TATATTTCAT
TGTGCAAGTA
GATAGAAATT
ACATATGTTA
CATAACACAC
GAAATAAACA
AAAAAAGACA
ATCCAAAAAC
AAACACCCCA
AAAAAAATAA
TCACTTTAGA
TAAACTCGTA
TGAGGAGAGG
CACGTTCAGT
GACTCGACGA
TTCCCGAGCA
AAAAAAGTCT
CCCCGTCACA
CATGTAGTGG
GTGACGCAAT
TATCTTTAAA
GTAATCCTTC
TGTTGACTTG
TCATTGATAA
CATCCAGTCT
TCGTCAGGAT
TGCAAAGAAT
TATAGAAGGG
ATCCCACCTT
TTATTTTCTT
CTTTTTTCCA
TATTTAGGGT
TGACAGTGAA
ATCAGACTGG
CAACCTATTA
ATTGCTTCCA
CAATGGGACG
AACTTGAAGG
GGATGTCGTC
GATGATATTA
TAGGTGGCGT
GTTCATCGTA
GTTGGTGAAA
TCGATGGTAC
CGTTCCAATA
GTTGTGTCGT
CCGAGACTTC
TAGCCCAGGT
GGTCTTTCCG
GTACGAGTTG
GTCCGCAGAT
GTAGAGGCTG
GGGTGTCGGA
TTCCATTCCT
TCCATTGTCC
TTGTTAAATC
GGCCATCCAT
TCAAGGTCAG
ATTGAGCTTG
TTGGTATGAG
ACAGGATGTA
TGTAAGTATA
AGCGTCTATG
CTTACATGGT
ATAGATGGGT
TTCCCTCCAG
GAGTGTAGAT
CTTCGTGGCA
GCGAAGATCT
GATTCTGTGA
AGGGCGACAC
ATACGGTTCA
GGTTGTGGAG
GGAATAATTT
GTTGGCTGAA
TATTCCAGCC
ATTGAAGCTT
TGTTGCCCAT
TCATGAGGGA
ATTCTTCCTT
GATCATGTCA
AGATATTCCT
CCTTAGACGT
TGCAGTCTGG
ATAATAGTTC
TCCATCGTGC
GTCAGATTTG
CGAGGAGAAA
CCTTATGATC
TCGGAAATCT
CCTCTGGTTT
TAATATCTCC
GTCCTTTGAT
ATGTAATCAA
GGACTTGTTT
AGAGTTTCTA
GCTGGCTGGA
TATTAGGGTG
ATTTCCTTCA
AAATCGAAAA
AAGAAGGATC
CCTAATACAA
GGTTTTTTAT
CAAGCTGGAG
AAGAGCATGA
TAGTGGGTAG
TGCCATCTTG
ATGAAGCTCA
GAAGCAACAC
CAAGGAAGAA
AATAAGAAAA
GGTGTGAGTT
TCTCCCAGAG
AAACTGGAAT
AAATCATCTC
TTTGAGATGA
GCACTTGGGA
TAGGTAAGGA
AAACATATTT
AGATTGGAGT
CTGAAGTTCT
TACTAGCAGA
AGGCATGTTG
TTGTGACTCC
GAGGGGTTGC
CTCAAACTCT
ATCTTATAAC
CGGCGTGGAG
GCATGGAGGC
AGGGGTATTT
TGGTCATTTT
AATAGATAGT
GGAAAATGAC
GTGGAATTTA
CTTAAAGACG
AAGTCTTTGC
GACAAGGGGG
GGCCCACGCC
GAATTTAATA
TTACCGGCGT
GGCCCCCCCT
TATCGCGAGT
GCTTTAGCAC
GAGCGGTCCA
GATTTAAAGT
AGAAAATTTC
CCGCCCACTA
GGGTTAAAGG
TGTTCACACT
ATAAAAGCAT
ATACGATGTG
ATGGTATTTG
ATGGAGCGTA
TATTGTATCA
GGTATTTCCG
TTGGATACGA
ATTATTCGTA
CGACCCTCAT
AGTTTAAACT
ATCAGTGTTT
GACAGGATAT
ATTGGCGGGT
AAACCTAAGA
GAAAAGAGCG
TTTATTAGAA
TAACGGATAT
TTAAAAGGGC
GTGAAAAGGT
TTATCCGTTC
GTCCATTTGT
ATGTGCATGC
CAACCACAGG
GTTCCCCTCG
GGATCAAAGT
ACTTTGATCC
AACCCCTCCG
CTGCTATAGT
GCAGTCGGCT
TCTGACGTTC
AGTGCAGCCG
TCTTCTGAAA
ACGACATGTC
GCACAAGTCC
TAAGTTACGC
GACAGGCTGC
CGCCCTGCCC
TTTTCCTGGC
GTTTTCTTGT
CGCGTGTTTT
AGTCGCATAA
AGTAGAATAC
TTGCGACTAG
AACCGGAGAC
ATTACGCCAT
GAACAAGAGC
GCCGCCGCTG
GCCTGCTGGG
CTATGCCCGC
GTCAGCACCG
ACGACCAGGA
CTTGACCAAC
CAACGGGCCG
AACTGCACGC
GGCCGGCTGC
ACCAAGCTGT
TTTCCGAGAA
GATCACCGGC
ACCAGGCGCG
ACCGCCCGGA
GCTGGCCAGG
ATGCTTGACC
ACCTACGCCC
TGGCGACGTT
GTGACAGTGA
CCAGGCTAGA
CCGCCTGGCC
CGCAGCACCC
GCGACCTACT
GGACATTGCC
GAGCGCATCC
AGGAGGCCGG
CGCGGGCCTG
CGTAGCCTGG
CAGAGCCGTG
GGCCGACACC
ACCACGCCGG
CCGGCCGCAT
GGTGTTGACC
GTGTTCGCCG
GCATTGCCGA
GTTCGAGCGT
TCCCTAATCA
TCGACCGCAC
CCGGAGCGGG
CGCGAGGCCG
CCAAGGCCCG
AGGCGTGAAG
TTTGGCCCCC
GCCCTACCCT
CACCCCGGCA
CAGATCGCGC
ACGCCCGCGA
GCTGATCGAC
CAGGAAGGCC
GCACCGTGAA
AGAGGCGGCT
GCACTGCTTG
GCGTGCATCG
CTCGACCCTG
TACCGCGCAC
TTGAGCGCAG
CGAGGAAGTG
ACGCCCACCG
AGGCCAGGCG
GCGCGGTGCC
TTCCGTGAGG
ACGCATTGAC
CGAGGCCGAC
GCCCTGGCGG
CCGCCGAGAA
TGAACGCCAA
GAGGAACAAG
CATGAAACCG
CACCAGGACG
GCCAGGACGA
ACCGTTTTTC
ATTACCGAAG
AGATCGAGGC
GGAGATGATC
GCGGCCGGGT
ACGTGTTCGA
GCCGCCCGCG
CACGGCTCAA
CCGTGCGGCT
GCATGAAATC
CTGGCCGGTT
TGTCTGATGC
CAAGCTGGCG
GCCTGGCCGG
CCAGCTTGGC
CGCTGAAGAA
ACCGAGCGCC
GCCGTCTAAA
AAGGTGATGT
GTATTTGAGT
AAAACAGCTT
GCGTCATGCG
GTCGCTGCGT
ATATGATGCG
ATGAGTAAAT
AAACAAATAC
GCAAGGGGAA
CGCATGAAGG
TTATCGCTGT
ACTTAACCAG
AAAGGCGGGT
CAGGCAAGAC
GACCATCGCA
ACCCATCTAG
CCCGCGCCCT
GCAACTCGCC
GGGGCCGATG
TTCTGTTAGT
CGATTCCGAT
CCCCAGGGCA
GTGCCCGCGA
TTGGGCGGCC
GTGCGGGAAG
ATCAACCGCT
AACCGTTGTC
GGCATCGACC
GCCCGACGAT
TGACCGCGAC
GTGAAGGCCA
TCGGCCGGCG
CGACTTCGTA
GTGATCGACG
GAGCGCCCCA
GGCGGCGGAC
TTGGCTGTGT
CCGCGATCAA
GGCAGCCGAC
TTCGTGCTGA
TTCCGGTGCA
GCCAAGCCCT
TACGACATAT
GGGCCACCGC
CGACCTGGTG
GAGCTGGTTA
AGCAGCGCAT
TGAGGTCACG
GATGGAAGGC
TACAAGCGGC
CTTTGTCGTG
TCGCGGGCGA
TCAAAGGCAC
GCGCATCGGC
GGTGAGGTTG
CCGAGGCGCT
GGCCGGGTAC
GAGCTGCCCA
TTCTTGAGTC
CCGTATCACG
CAGCGCGTGA
GCTACCCAGG
CACTGCCGCC
GCCGGCACAA
CCGTTCTTGA
ATCAGAACCC
GAGGGCGACG
CTGCCCGCGA
GGTCCAGGCG
CTGGCCGCTG
AAATTAAATC
AAAACTCATT
TGAGTTAATG
AGGTAAAGAG
AAAATGAGCA
AAAGCACAAA
CACGCTAAGT
GCCGGCCGTC
CGAGCGCACG
CAGCAGCAAG
GCTGCAACGT
TGGCCAGCCT
GGCAGACACG
CCAGCCATGA
AGCGGGTCAA
CTTTCAGTTG
CCGGCGGAGG
ATCACACCAA
GCTGAAGATG
TACGCGGTAC
GCCAAGGCAA
GACCATTACC
GAGCTGCTAT
CTGAATACAT
CGCGCAGCTA
CCAGAGTAAA
TGAGCAAATG
AATAAATGAG
TAGATGAATT
TTAGCGGCTA
AAGGAGGCGG
CATGGAAAAT
CAAGAACAAC
CAGGCACCGA
CGCCGTGGAA
TGCCCCATGT
GTGGAGGAAC
GGGCGGTTGG
CCAGGCGTAA
GCGGCTGGGT
TGTCTGCCGG
CCCTGCAATG
GCACTGGAAC
CCCCAAGCCC
GAGGAATCGG
CGTGACGGTC
GCAAACCATC
CGGCCCGGTA
CAAATCGGCG
CGGCGCTGGG
TGATGACCTG
GTGGAGAAGT
TGAAGGCCGC
GCAGGCCGCC
CAGCGGCAAC
GCATCGAGGC
AGAAGCACGC
CCCGGTGAAT
CGTGGCAAGC
GGCCGCTGAT
CGAATCCGCA
AAGAATCCCG
GCAACCGCCG
GCAGCCGGTG
CGCCGTCGAT
TAGGAAGCCG
CCCAAGGGCG
ACGAGCAACC
AGATTTTTTC
GTTCCGATGC
TCTATGACGT
GGGCACCCGC
GATAGTCGCA
GCATCATGGA
CGTGGCCGTT
TTCCGTCTGT
CGAAGCGTGA
CCGACGAGCT
GGCGAGGTGA
TCCGCTACGA
GCTTCCAGAC
GGGCACGTAG
AGGTTTCCGC
AGGGCCGGCC
GGCATGGCCA
GTGTGTGGGA
TTACGACCTG
GTACTGATGG
CGGTTTCCCA
TCTAACCGAA
TCCATGAACC
GATACCGGGA
AGGGAAGGGA
GACAAGCCCG
GCCGCGTGTT
CCGTCCACAC
GTTGCGGACG
TACTCAAGTT
CTGCCGGCGA
GCCGATGGCG
GAAAGCAGAA
AGACGACCTG
GTAGAAACCT
GCATTCGGTT
AAACACCACG
CACGTTGCCA
TGCAGCGTAC
GAAGAAGGCC
AAGAACGGCC
GCCTGGTGAC
GGTATCCGAG
GGTGAAGCCT
TGATTAGCCG
CTACAAGATC
GTAAAGAGCG
AAACCGGGCG
GCCGGAGTAC
ATCGAGATCG
AGCTAGCTGA
TTGGATGTAC
CGCGAGATCA
CAGAAGGCAA
GAACCCGGAC
GTGCTGACGG
TTCACCCCGA
TTACTTTTTG
ATCGATCCCG
GCATCGGCCG
TTTTCTCTAC
CGCCTGGCAC
GCCGCGCCGC
AGGCAAGGCA
GAAGCCAGAT
GGTTGTTCAA
GACGATCTAC
GAACGCAGTG
GCAGCGCCGG
AGAGTTCAAG
AAGTTCTGTT
TCACCGTGCG
CAAGCTGATC
GGGTCAAATG
ACCTGCCGGA
GTACGATTTG
AAGGAGGAGG
CGGGGCAGGC
TGGCCCGATC
CTAGTCATGC
GCTACCGCAA
CCTGATCGAG
GGCGAAGCAT
CCGCCGGTTC
CTAATGTACG
GAGCAGATGC
TAGGGCAAAT
TGCCCTAGCA
GGGGAAAAAG
GTCGAAAAGG
CCTCTTTCCT
GTGGATAGCA
CGTACATTGG
GAACCCAAAG
CCGTACATTG
GGAACCGGAA
CCCGTACATT
GGGAACCCAA
AGCCGTACAT
TGGGAACCGG
TCACACATGT
AAGTGACTGA
TATAAAAGAG
AAAAAAGGCG
ATTTTTCCGC
CTAAAACTCT
TTAAAACTTA
TTAAAACTCT
TAAAACCCGC
CTGGCCTGTG
CATAACTGTC
TGGCCAGCGC
ACAGCCGAAG
AGCTGCAAAA
AGCGCCTACC
CTTCGGTCGC
TGCGCTCCCT
ACGCCCCGCC
GCTTCGCGTC
GGCCTATCGC
GGCCGCTGGC
CGCTCAAAAA
TGGCTGGCCT
ACGGCCAGGC
AATCTACCAG
GGCGCGGACA
AGCCGCGCCG
TCGCCACTCG
ACCGCCGGCG
CCCACATCAA
GGCACCCTGC
CTCGCGCGTT
TCGGTGATGA
CGGTGAAAAC
CTCTGACACA
TGCAGCTCCC
GGAAACGGTC
ACAGCTTGTC
TGTAAGCGGA
TGCCGGGAGC
AGACAAGCCC
GTCAGGGCGC
GTCAGCGGGT
GTTGGCGGGT
GTCGGGGCGC
AGCCATGACC
CAGTCACGTA
GCGATAGCGG
AGTGTATACT
GGCTTAACTA
TGCGGCATCA
GAGCAGATTG
TACTGAGAGT
GCACCATATG
CGGTGTGAAA
TACCGCACAG
ATGCGTAAGG
AGAAAATACC
GCATCAGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGGACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGCAT
TCTAGGTACT
AAAACAATTC
ATCCAGTAAA
ATATAATATT
TTATTTTCTC
CCAATCAGGC
TTGATCCCCA
GTAAGTCAAA
AAATAGCTCG
ACATACTGTT
CTTCCCCGAT
ATCCTCCCTG
ATCGACCGGA
CGCAGAAGGC
AATGTCATAC
CACTTGTCCG
CCCTGCCGCT
TCTCCCAAGA
TCAATAAAGC
CACTTACTTT
GCCATCTTTC
ACAAAGATGT
TGCTGTCTCC
CAGGTCGCCG
TGGGAAAAGA
CAAGTTCCTC
TTCGGGCTTT
TCCGTCTTTA
AAAAATCATA
CAGCTCGCGC
GGATCTTTAA
ATGGAGTGTC
TTCTTCCCAG
TTTTCGCAAT
CCACATCGGC
CAGATCGTTA
TTCAGTAAGT
AATCCAATTC
GGCTAAGCGG
CTGTCTAAGC
TATTCGTATA
GGGACAATCC
GATATGTCGA
TGGAGTGAAA
GAGCCTGATG
CACTCCGCAT
ACAGCTCGAT
AATCTTTTCA
GGGCTTTGTT
CATCTTCATA
CTCTTCCGAG
CAAAGGACGC
CATCGGCCTC
ACTCATGAGC
AGATTGCTCC
AGCCATCATG
CCGTTCAAAG
TGCAGGACCT
TTGGAACAGG
CAGCTTTCCT
TCCAGCCATA
GCATCATGTC
CTTTTCCCGT
TCCACATCAT
AGGTGGTCCC
TTTATACCGG
CTGTCCGTCA
TTTTTAAATA
TAGGTTTTCA
TTTTCTCCCA
CCAGCTTATA
TACCTTAGCA
GGAGACATTC
CTTCCGTATC
TTTTACGCAG
CGGTATTTTT
CGATCAGTTT
TTTCAATTCC
GGTGATATTC
TCATTTTAGC
CATTTATTAT
TTCCTTCCTC
TTTTCTACAG
TATTTAAAGA
TACCCCAAGA
AGCTAATTAT
AACAAGACGA
ACTCCAATTC
ACTGTTCCTT
GCATTCTAAA
ACCTTAAATA
CCAGAAAACA
GCTTTTTCAA
AGTTGTTTTC
AAAGTTGGCG
TATAACATAG
TATCGACGGA
GCCGATTTTG
AAACCGCGGT
GATCACAGGC
AGCAACGCTC
TGTCATCGTT
ACAATCAACA
TGCTACCCTC
CGCGAGATCA
TCCGTGTTTC
AAACCCGGCA
GCTTAGTTGC
CGTTCTTCCG
AATAGCATCG
GTAACATGAG
CAAAGTCTGC
CGCCTTACAA
CGGCTCTCCC
GCTGACGCCG
TCCCGGACTG
ATGGGCTGCC
TGTATCGAGT
GGTGATTTTG
TGCCGAGCTG
CCGGTCGGGG
AGCTGTTGGC
TGGCTGG