名称: | pLL3.7-Hsa-Let-7a-1miRNA |
---|---|
别称: | pre--Hsa-Let-7a-1 |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 信号报告 |
片段类型: | miRNA |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTCGACGGAT
CGGGAGATCT
CCCGATCCCC
TATGGTGCAC
TCTCAGTACA
ATCTGCTCTG
ATGCCGCATA
GTTAAGCCAG
TATCTGCTCC
CTGCTTGTGT
GTTGGAGGTC
GCTGAGTAGT
GCGCGAGCAA
AATTTAAGCT
ACAACAAGGC
AAGGCTTGAC
CGACAATTGC
ATGAAGAATC
TGCTTAGGGT
TAGGCGTTTT
GCGCTGCTTC
GCGATGTACG
GGCCAGATAT
ACGCGTTGAC
ATTGATTATT
GACTAGTTAT
TAATAGTAAT
CAATTACGGG
GTCATTAGTT
CATAGCCCAT
ATATGGAGTT
CCGCGTTACA
TAACTTACGG
TAAATGGCCC
GCCTGGCTGA
CCGCCCAACG
ACCCCCGCCC
ATTGACGTCA
ATAATGACGT
ATGTTCCCAT
AGTAACGCCA
ATAGGGACTT
TCCATTGACG
TCAATGGGTG
GAGTATTTAC
GGTAAACTGC
CCACTTGGCA
GTACATCAAG
TGTATCATAT
GCCAAGTACG
CCCCCTATTG
ACGTCAATGA
CGGTAAATGG
CCCGCCTGGC
ATTATGCCCA
GTACATGACC
TTATGGGACT
TTCCTACTTG
GCAGTACATC
TACGTATTAG
TCATCGCTAT
TACCATGGTG
ATGCGGTTTT
GGCAGTACAT
CAATGGGCGT
GGATAGCGGT
TTGACTCACG
GGGATTTCCA
AGTCTCCACC
CCATTGACGT
CAATGGGAGT
TTGTTTTGGC
ACCAAAATCA
ACGGGACTTT
CCAAAATGTC
GTAACAACTC
CGCCCCATTG
ACGCAAATGG
GCGGTAGGCG
TGTACGGTGG
GAGGTCTATA
TAAGCAGCGC
GTTTTGCCTG
TACTGGGTCT
CTCTGGTTAG
ACCAGATCTG
AGCCTGGGAG
CTCTCTGGCT
AACTAGGGAA
CCCACTGCTT
AAGCCTCAAT
AAAGCTTGCC
TTGAGTGCTT
CAAGTAGTGT
GTGCCCGTCT
GTTGTGTGAC
TCTGGTAACT
AGAGATCCCT
CAGACCCTTT
TAGTCAGTGT
GGAAAATCTC
TAGCAGTGGC
GCCCGAACAG
GGACTTGAAA
GCGAAAGGGA
AACCAGAGGA
GCTCTCTCGA
CGCAGGACTC
GGCTTGCTGA
AGCGCGCACG
GCAAGAGGCG
AGGGGCGGCG
ACTGGTGAGT
ACGCCAAAAA
TTTTGACTAG
CGGAGGCTAG
AAGGAGAGAG
ATGGGTGCGA
GAGCGTCAGT
ATTAAGCGGG
GGAGAATTAG
ATCGCGATGG
GAAAAAATTC
GGTTAAGGCC
AGGGGGAAAG
AAAAAATATA
AATTAAAACA
TATAGTATGG
GCAAGCAGGG
AGCTAGAACG
ATTCGCAGTT
AATCCTGGCC
TGTTAGAAAC
ATCAGAAGGC
TGTAGACAAA
TACTGGGACA
GCTACAACCA
TCCCTTCAGA
CAGGATCAGA
AGAACTTAGA
TCATTATATA
ATACAGTAGC
AACCCTCTAT
TGTGTGCATC
AAAGGATAGA
GATAAAAGAC
ACCAAGGAAG
CTTTAGACAA
GATAGAGGAA
GAGCAAAACA
AAAGTAAGAC
CACCGCACAG
CAAGCGGCCG
GCCGCGCTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCG
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTTGG
AATCACACGA
CCTGGATGGA
GTGGGACAGA
GAAATTAACA
ATTACACAAG
CTTAATACAC
TCCTTAATTG
AAGAATCGCA
AAACCAGCAA
GAAAAGAATG
AACAAGAATT
ATTGGAATTA
GATAAATGGG
CAAGTTTGTG
GAATTGGTTT
AACATAACAA
ATTGGCTGTG
GTATATAAAA
TTATTCATAA
TGATAGTAGG
AGGCTTGGTA
GGTTTAAGAA
TAGTTTTTGC
TGTACTTTCT
ATAGTGAATA
GAGTTAGGCA
GGGATATTCA
CCATTATCGT
TTCAGACCCA
CCTCCCAACC
CCGAGGGGAC
CCGACAGGCC
CGAAGGAATA
GAAGAAGAAG
GTGGAGAGAG
AGACAGAGAC
AGATCCATTC
GATTAGTGAA
CGGATCGGCA
CTGCGTGCGC
CAATTCTGCA
GACAAATGGC
AGTATTCATC
CACAATTTTA
AAAGAAAAGG
GGGGATTGGG
GGGTACAGTG
CAGGGGAAAG
AATAGTAGAC
ATAATAGCAA
CAGACATACA
AACTAAAGAA
TTACAAAAAC
AAATTACAAA
AATTCAAAAT
TTTCGGGTTT
ATTACAGGGA
CAGCAGAGAT
CCAGTTTGGT
TAGTACCGGG
CCCGCTCTAG
AGATCCGACG
CCGCCATCTC
TAGGCCCGCG
CCGGCCCCCT
CGCACAGACT
TGTGGGAGAA
GCTCGGCTAC
TCCCCTGCCC
CGGTTAATTT
GCATATAATA
TTTCCTAGTA
ACTATAGAGG
CTTAATGTGC
GATAAAAGAC
AGATAATCTG
TTCTTTTTAA
TACTAGCTAC
ATTTTACATG
ATAGGCTTGG
ATTTCTATAA
GAGATACAAA
TACTAAATTA
TTATTTTAAA
AAACAGCACA
AAAGGAAACT
CACCCTAACT
GTAAAGTAAT
TGTGTGTTTT
GAGACTATAA
ATATCCCTTG
GAGAAAAGCC
TTGTTAACGC
GCGGTGACCC
TCGACTTCAA
CGTAAGTGAA
TGAAAATGGT
CTAATACTAT
TTTTCTTATC
ACTCACACAG
GAAACCAGGA
TTACCGAGGA
GGAAAAAAAG
CCTTCCTGTG
GTGCTCAACT
GTGATTCCTT
TTCACCATTC
ACCCTGGATG
TTCTCTTCAC
TGTGGGATGA
GGTAGTAGGT
TGTATAGTTT
TAGGGTCACA
CCCACCACTG
GGAGATAACT
ATACAATCTA
CTGTCTTTCC
TAACGTGATA
GAAAAGTCTG
CATCCAGGCG
GTCTGATAGA
AAGTCAGTTA
ACTAATTGTA
CAATATTTAA
GATTAACTTG
TCTTAAAGAG
ATGTAGTGCA
GCATTTGTTT
ATGGCCTGGA
AATAGGATCC
TTTTTATTTA
ACGCGAATTA
ATTCTGTGGA
ATGTGTGTCA
GTTAGGGTGT
GGAAAGTCCC
CAGGCTCCCC
AGCAGGCAGA
AGTATGCAAA
GCATGCATCT
CAATTAGTCA
GCAACCAGGT
GTGGAAAGTC
CCCAGGCTCC
CCAGCAGGCA
GAAGTATGCA
AAGCATGCAT
CTCAATTAGT
CAGCAACCAT
AGTCCCGCCC
CTAACTCCGC
CCATCCCGCC
CCTAACTCCG
CCCAGTTCCG
CCCATTCTCC
GCCCCATGGC
TGACTAATTT
TTTTTATTTA
TGCAGAGGCC
GAGGCCGCCT
CTGCCTCTGA
GCTATTCCAG
AAGTAGTGAG
GAGGCTTTTT
TGGAGGCCTA
GGCTTTTGCA
AAAAGCTCCC
GGCTAGCATG
ACCGAGTACA
AGCCCACGGT
GCGCCTCGCC
ACCCGCGACG
ACGTCCCCAG
GGCCGTACGC
ACCCTCGCCG
CCGCGTTCGC
CGACTACCCC
GCCACGCGCC
ACACCGTCGA
TCCGGACCGC
CACATCGAGC
GGGTCACCGA
GCTGCAAGAA
CTCTTCCTCA
CGCGCGTCGG
GCTCGACATC
GGCAAGGTGT
GGGTCGCGGA
CGACGGCGCC
GCGGTGGCGG
TCTGGACCAC
GCCGGAGAGC
GTCGAAGCGG
GGGCGGTGTT
CGCCGAGATC
GGCCCGCGCA
TGGCCGAGTT
GAGCGGTTCC
CGGCTGGCCG
CGCAGCAACA
GATGGAAGGC
CTCCTGGCGC
CGCACCGGCC
CAAGGAGCCC
GCGTGGTTCC
TGGCCACCGT
CGGCGTCTCG
CCCGACCACC
AGGGCAAGGG
TCTGGGCAGC
GCCGTCGTGC
TCCCCGGAGT
GGAGGCGGCC
GAGCGCGCCG
GGGTGCCCGC
CTTCCTGGAG
ACCTCCGCGC
CCCGCAACCT
CCCCTTCTAC
GAGCGGCTCG
GCTTCACCGT
CACCGCCGAC
GTCGAGGTGC
CCGAAGGACC
GCGCACCTGG
TGCATGACCC
GCAAGCCCGG
TGCCTGAGAA
TTCGTCGAGG
TCGACGGTAT
CGATAAGCTC
GCTTCACGAG
ATTCCAGCAG
GTCGAGGGAC
CTAATAACTT
CGTATAGCAT
ACATTATACG
AAGTTATATT
AAGGGTTCCA
AGCTTAAGCG
GCCGCGTGGA
TAACCGTATT
ACCGCCATGC
ATTAGTTATT
AATAGTAATC
AATTACGGGG
TCATTAGTTC
ATAGCCCATA
TATGGAGTTC
CGCGTTACAT
AACTTACGGT
AAATGGCCCG
CCTGGCTGAC
CGCCCAACGA
CCCCCGCCCA
TTGACGTCAA
TAATGACGTA
TGTTCCCATA
GTAACGCCAA
TAGGGACTTT
CCATTGACGT
CAATGGGTGG
AGTATTTACG
GTAAACTGCC
CACTTGGCAG
TACATCAAGT
GTATCATATG
CCAAGTACGC
CCCCTATTGA
CGTCAATGAC
GGTAAATGGC
CCGCCTGGCA
TTATGCCCAG
TACATGACCT
TATGGGACTT
TCCTACTTGG
CAGTACATCT
ACGTATTAGT
CATCGCTATT
ACCATGGTGA
TGCGGTTTTG
GCAGTACATC
AATGGGCGTG
GATAGCGGTT
TGACTCACGG
GGATTTCCAA
GTCTCCACCC
CATTGACGTC
AATGGGAGTT
TGTTTTGGCA
CCAAAATCAA
CGGGACTTTC
CAAAATGTCG
TAACAACTCC
GCCCCATTGA
CGCAAATGGG
CGGTAGGCGT
GTACGGTGGG
AGGTCTATAT
AAGCAGAGCT
GGTTTAGTGA
ACCGTCAGAT
CCGCTAGCGC
TACCGGTCGC
CACCATGGTG
AGCAAGGGCG
AGGAGCTGTT
CACCGGGGTG
GTGCCCATCC
TGGTCGAGCT
GGACGGCGAC
GTAAACGGCC
ACAAGTTCAG
CGTGTCCGGC
GAGGGCGAGG
GCGATGCCAC
CTACGGCAAG
CTGACCCTGA
AGTTCATCTG
CACCACCGGC
AAGCTGCCCG
TGCCCTGGCC
CACCCTCGTG
ACCACCCTGA
CCTACGGCGT
GCAGTGCTTC
AGCCGCTACC
CCGACCACAT
GAAGCAGCAC
GACTTCTTCA
AGTCCGCCAT
GCCCGAAGGC
TACGTCCAGG
AGCGCACCAT
CTTCTTCAAG
GACGACGGCA
ACTACAAGAC
CCGCGCCGAG
GTGAAGTTCG
AGGGCGACAC
CCTGGTGAAC
CGCATCGAGC
TGAAGGGCAT
CGACTTCAAG
GAGGACGGCA
ACATCCTGGG
GCACAAGCTG
GAGTACAACT
ACAACAGCCA
CAACGTCTAT
ATCATGGCCG
ACAAGCAGAA
GAACGGCATC
AAGGTGAACT
TCAAGATCCG
CCACAACATC
GAGGACGGCA
GCGTGCAGCT
CGCCGACCAC
TACCAGCAGA
ACACCCCCAT
CGGCGACGGC
CCCGTGCTGC
TGCCCGACAA
CCACTACCTG
AGCACCCAGT
CCGCCCTGAG
CAAAGACCCC
AACGAGAAGC
GCGATCACAT
GGTCCTGCTG
GAGTTCGTGA
CCGCCGCCGG
GATCACTCTC
GGCATGGACG
AGCTGTACAA
GTAGGAATTC
GTCGAGGGAC
CTAATAACTT
CGTATAGCAT
ACATTATACG
AAGTTATACA
TGTTTAAGGG
TTCCGGTTCC
ACTAGGTACA
ATTCGATATC
AAGCTTATCG
ATAATCAACC
TCTGGATTAC
AAAATTTGTG
AAAGATTGAC
TGGTATTCTT
AACTATGTTG
CTCCTTTTAC
GCTATGTGGA
TACGCTGCTT
TAATGCCTTT
GTATCATGCT
ATTGCTTCCC
GTATGGCTTT
CATTTTCTCC
TCCTTGTATA
AATCCTGGTT
GCTGTCTCTT
TATGAGGAGT
TGTGGCCCGT
TGTCAGGCAA
CGTGGCGTGG
TGTGCACTGT
GTTTGCTGAC
GCAACCCCCA
CTGGTTGGGG
CATTGCCACC
ACCTGTCAGC
TCCTTTCCGG
GACTTTCGCT
TTCCCCCTCC
CTATTGCCAC
GGCGGAACTC
ATCGCCGCCT
GCCTTGCCCG
CTGCTGGACA
GGGGCTCGGC
TGTTGGGCAC
TGACAATTCC
GTGGTGTTGT
CGGGGAAATC
ATCGTCCTTT
CCTTGGCTGC
TCGCCTGTGT
TGCCACCTGG
ATTCTGCGCG
GGACGTCCTT
CTGCTACGTC
CCTTCGGCCC
TCAATCCAGC
GGACCTTCCT
TCCCGCGGCC
TGCTGCCGGC
TCTGCGGCCT
CTTCCGCGTC
TTCGCCTTCG
CCCTCAGACG
AGTCGGATCT
CCCTTTGGGC
CGCCTCCCCG
CATCGATACC
GTCGACCTCG
ATCGAGACCT
AGAAAAACAT
GGAGCAATCA
CAAGTAGCAA
TACAGCAGCT
ACCAATGCTG
ATTGTGCCTG
GCTAGAAGCA
CAAGAGGAGG
AGGAGGTGGG
TTTTCCAGTC
ACACCTCAGG
TACCTTTAAG
ACCAATGACT
TACAAGGCAG
CTGTAGATCT
TAGCCACTTT
TTAAAAGAAA
AGGGGGGACT
GGAAGGGCTA
ATTCACTCCC
AACGAAGACA
AGATATCCTT
GATCTGTGGA
TCTACCACAC
ACAAGGCTAC
TTCCCTGATT
GGCAGAACTA
CACACCAGGG
CCAGGGATCA
GATATCCACT
GACCTTTGGA
TGGTGCTACA
AGCTAGTACC
AGTTGAGCAA
GAGAAGGTAG
AAGAAGCCAA
TGAAGGAGAG
AACACCCGCT
TGTTACACCC
TGTGAGCCTG
CATGGGATGG
ATGACCCGGA
GAGAGAAGTA
TTAGAGTGGA
GGTTTGACAG
CCGCCTAGCA
TTTCATCACA
TGGCCCGAGA
GCTGCATCCG
GACTGTACTG
GGTCTCTCTG
GTTAGACCAG
ATCTGAGCCT
GGGAGCTCTC
TGGCTAACTA
GGGAACCCAC
TGCTTAAGCC
TCAATAAAGC
TTGCCTTGAG
TGCTTCAAGT
AGTGTGTGCC
CGTCTGTTGT
GTGACTCTGG
TAACTAGAGA
TCCCTCAGAC
CCTTTTAGTC
AGTGTGGAAA
ATCTCTAGCA
GCATGTGAGC
AAAAGGCCAG
CAAAAGGCCA
GGAACCGTAA
AAAGGCCGCG
TTGCTGGCGT
TTTTCCATAG
GCTCCGCCCC
CCTGACGAGC
ATCACAAAAA
TCGACGCTCA
AGTCAGAGGT
GGCGAAACCC
GACAGGACTA
TAAAGATACC
AGGCGTTTCC
CCCTGGAAGC
TCCCTCGTGC
GCTCTCCTGT
TCCGACCCTG
CCGCTTACCG
GATACCTGTC
CGCCTTTCTC
CCTTCGGGAA
GCGTGGCGCT
TTCTCATAGC
TCACGCTGTA
GGTATCTCAG
TTCGGTGTAG
GTCGTTCGCT
CCAAGCTGGG
CTGTGTGCAC
GAACCCCCCG
TTCAGCCCGA
CCGCTGCGCC
TTATCCGGTA
ACTATCGTCT
TGAGTCCAAC
CCGGTAAGAC
ACGACTTATC
GCCACTGGCA
GCAGCCACTG
GTAACAGGAT
TAGCAGAGCG
AGGTATGTAG
GCGGTGCTAC
AGAGTTCTTG
AAGTGGTGGC
CTAACTACGG
CTACACTAGA
AGAACAGTAT
TTGGTATCTG
CGCTCTGCTG
AAGCCAGTTA
CCTTCGGAAA
AAGAGTTGGT
AGCTCTTGAT
CCGGCAAACA
AACCACCGCT
GGTAGCGGTG
GTTTTTTTGT
TTGCAAGCAG
CAGATTACGC
GCAGAAAAAA
AGGATCTCAA
GAAGATCCTT
TGATCTTTTC
TACGGGGTCT
GACGCTCAGT
GGAACGAAAA
CTCACGTTAA
GGGATTTTGG
TCATGAGATT
ATCAAAAAGG
ATCTTCACCT
AGATCCTTTT
AAATTAAAAA
TGAAGTTTTA
AATCAATCTA
AAGTATATAT
GAGTAAACTT
GGTCTGACAG
TTACCAATGC
TTAATCAGTG
AGGCACCTAT
CTCAGCGATC
TGTCTATTTC
GTTCATCCAT
AGTTGCCTGA
CTCCCCGTCG
TGTAGATAAC
TACGATACGG
GAGGGCTTAC
CATCTGGCCC
CAGTGCTGCA
ATGATACCGC
GAGACCCACG
CTCACCGGCT
CCAGATTTAT
CAGCAATAAA
CCAGCCAGCC
GGAAGGGCCG
AGCGCAGAAG
TGGTCCTGCA
ACTTTATCCG
CCTCCATCCA
GTCTATTAAT
TGTTGCCGGG
AAGCTAGAGT
AAGTAGTTCG
CCAGTTAATA
GTTTGCGCAA
CGTTGTTGCC
ATTGCTACAG
GCATCGTGGT
GTCACGCTCG
TCGTTTGGTA
TGGCTTCATT
CAGCTCCGGT
TCCCAACGAT
CAAGGCGAGT
TACATGATCC
CCCATGTTGT
GCAAAAAAGC
GGTTAGCTCC
TTCGGTCCTC
CGATCGTTGT
CAGAAGTAAG
TTGGCCGCAG
TGTTATCACT
CATGGTTATG
GCAGCACTGC
ATAATTCTCT
TACTGTCATG
CCATCCGTAA
GATGCTTTTC
TGTGACTGGT
GAGTACTCAA
CCAAGTCATT
CTGAGAATAG
TGTATGCGGC
GACCGAGTTG
CTCTTGCCCG
GCGTCAATAC
GGGATAATAC
CGCGCCACAT
AGCAGAACTT
TAAAAGTGCT
CATCATTGGA
AAACGTTCTT
CGGGGCGAAA
ACTCTCAAGG
ATCTTACCGC
TGTTGAGATC
CAGTTCGATG
TAACCCACTC
GTGCACCCAA
CTGATCTTCA
GCATCTTTTA
CTTTCACCAG
CGTTTCTGGG
TGAGCAAAAA
CAGGAAGGCA
AAATGCCGCA
AAAAAGGGAA
TAAGGGCGAC
ACGGAAATGT
TGAATACTCA
TACTCTTCCT
TTTTCAATAT
TATTGAAGCA
TTTATCAGGG
TTATTGTCTC
ATGAGCGGAT
ACATATTTGA
ATGTATTTAG
AAAAATAAAC
AAATAGGGGT
TCCGCGCACA
TTTCCCCGAA
AAGTGCCACC
TGAC