名称: | pDEST32 |
---|---|
别称: | |
基因长: | |
质粒宿主: | 酵母菌 |
质粒用途: | 杂交 |
片段类型: | ORF,GateWay |
片段物种: | 空载体 |
原核抗性: | Gen,Chl |
筛选标记: | LEU2 |
荧光标记: | |
启动子: | ADH1 |
复制子: | pUC |
拷贝数: | |
感受态: | DB3.1 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TATCTCTATG
TAAGCTACGT
GGCGTGACTT
TTGATGAAGC
CGCACAAGAG
ATACAGGATT
GGCAACTGCA
AATAGAATCT
GGGGATCCCC
CCTCGAGATC
CGGGATCGAA
GAAATGATGG
TAAATGAAAT
AGGAAATCAA
GGAGCATGAA
GGCAAAAGAC
AAATATAAGG
GTCGAACGAA
AAATAAAGTG
AAAAGTGTTG
ATATGATGTA
TTTGGCTTTG
CGGCGCCGAA
AAAACGAGTT
TACGCAATTG
CACAATCATG
CTGACTCTGT
GGCGGACCCG
CGCTCTTGCC
GGCCCGGCGA
TAACGCTGGG
CGTGAGGCTG
TGCCCGGCGG
AGTTTTTTGC
GCCTGCATTT
TCCAAGGTTT
ACCCTGCGCT
AAGGGGCGAG
ATTGGAGAAG
CAATAAGAAT
GCCGGTTGGG
GTTGCGATGA
TGACGACCAC
GACAACTGGT
GTCATTATTT
AAGTTGCCGA
AAGAACCTGA
GTGCATTTGC
AACATGAGTA
TACTAGAAGA
ATGAGCCAAG
ACTTGCGAGA
CGCGAGTTTG
CCGGTGGTGC
GAACAATAGA
GCGACCATGA
CCTTGAAGGT
GAGACGCGCA
TAACCGCTAG
AGTACTTTGA
AGAGGAAACA
GCAATAGGGT
TGCTACCAGT
ATAAATAGAC
AGGTACATAC
AACACTGGAA
ATGGTTGTCT
GTTTGAGTAC
GCTTTCAATT
CATTTGGGTG
TGCACTTTAT
TATGTTACAA
TATGGAAGGG
AACTTTACAC
TTCTCCTATG
CACATATATT
AATTAAAGTC
CAATGCTAGT
AGAGAAGGGG
GGTAACACCC
CTCCGCGCTC
TTTTCCGATT
TTTTTCTAAA
CCGTGGAATA
TTTCGGATAT
CCTTTTGTTG
TTTCCGGGTG
TACAATATGG
ACTTCCTCTT
TTCTGGCAAC
CAAACCCATA
CATCGGGATT
CCTATAATAC
CTTCGTTGGT
CTCCCTAACA
TGTAGGTGGC
GGAGGGGAGA
TATACAATAG
AACAGATACC
AGACAAGACA
TAATGGGCTA
AACAAGACTA
CACCAATTAC
ACTGCCTCAT
TGATGGTGGT
ACATAACGAA
CTAATACTGT
AGCCCTAGAC
TTGATAGCCA
TCATCATATC
GAAGTTTCAC
TACCCTTTTT
CCATTTGCCA
TCTATTGAAG
TAATAATAGG
CGCATGCAAC
TTCTTTTCTT
TTTTTTTCTT
TTCTCTCTCC
CCCGTTGTTG
TCTCACCATA
TCCGCAATGA
CAAAAAAATG
ATGGAAGACA
CTAAAGGAAA
AAATTAACGA
CAAAGACAGC
ACCAACAGAT
GTCGTTGTTC
CAGAGCTGAT
GAGGGGTATC
TCGAAGCACA
CGAAACTTTT
TCCTTCCTTC
ATTCACGCAC
ACTACTCTCT
AATGAGCAAC
GGTATACGGC
CTTCCTTCCA
GTTACTTGAA
TTTGAAATAA
AAAAAAGTTT
GCTGTCTTGC
TATCAAGTAT
AAATAGACCT
GCAATTATTA
ATCTTTTGTT
TCCTCGTCAT
TGTTCTCGTT
CCCTTTCTTC
CTTGTTTCTT
TTTCTGCACA
ATATTTCAAG
CTATACCAAG
CATACAATCA
ACTCCAAGCT
TGAAGCAAGC
CTCCTGAAAG
ATGAAGCTAC
TGTCTTCTAT
CGAACAAGCA
TGCGATATTT
GCCGACTTAA
AAAGCTCAAG
TGCTCCAAAG
AAAAACCGAA
GTGCGCCAAG
TGTCTGAAGA
ACAACTGGGA
GTGTCGCTAC
TCTCCCAAAA
CCAAAAGGTC
TCCGCTGACT
AGGGCACATC
TGACAGAAGT
GGAATCAAGG
CTAGAAAGAC
TGGAACAGCT
ATTTCTACTG
ATTTTTCCTC
GAGAAGACCT
TGACATGATT
TTGAAAATGG
ATTCTTTACA
GGATATAAAA
GCATTGTTAA
CAGGATTATT
TGTACAAGAT
AATGTGAATA
AAGATGCCGT
CACAGATAGA
TTGGCTTCAG
TGGAGACTGA
TATGCCTCTA
ACATTGAGAC
AGCATAGAAT
AAGTGCGACA
TCATCATCGG
AAGAGAGTAG
TAACAAAGGT
CAAAGACAGT
TGACTGTATC
GTCGAGGTCG
AATCAAACAA
GTTTGTACAA
AAAAGCTGAA
CGAGAAACGT
AAAATGATAT
AAATATCAAT
ATATTAAATT
AGATTTTGCA
TAAAAAACAG
ACTACATAAT
ACTGTAAAAC
ACAACATATC
CAGTCACTAT
GGCGGCCGCG
GGTGAGTCAG
CATCACCCGA
CGCACTTTGC
GCCGAATAAA
TACCTGTGAC
GGAAGATCAC
TTCGCAGAAT
AAATAAATCC
TGGTGTCCCT
GTTGATACCG
GGAAGCCCTG
GGCCAACTTT
TGGCGAAAAT
GAGACGTTGA
TCGGCACGTA
AGAGGTTCCA
ACTTTCACCA
TAATGAAATA
AGATCACTAC
CGGGCGTATT
TTTTGAGTCA
TCGAGATTTT
CAGGAGCTAA
GGAAGCTAAA
ATGGAGAAAA
AAATCACTGG
ATATACCACC
GTTGATATAT
CCCAATGGCA
TCGTAAAGAA
CATTTTGAGG
CATTTCAGTC
AGTTGCTCAA
TGTACCTATA
ACCAGACCGT
TCAGCTGGAT
ATTACGGCCT
TTTTAAAGAC
CGTAAAGAAA
AATAAGCACA
AGTTTTATCC
GGCCTTTATT
CACATTCTTG
CCCGCCTGAT
GAATGCTCAT
CCGGAATTCC
GTATGGCAAT
GAAAGACGGT
GAGCTGGTGA
TATGGGATAG
TGTTCACCCT
TGTTACACCG
TTTTCCATGA
GCAAACTGAA
ACGTTTTCAT
CGCTCTGGAG
TGAATACCAC
GACGATTTCC
GGCAGTTTCT
ACACATATAT
TCGCAAGATG
TGGCGTGTTA
CGGTGAAAAC
CTGGCCTATT
TCCCTAAAGG
GTTTATTGAG
AATATGTTTT
TCGTCTCAGC
CAATCCCTGG
GTGAGTTTCA
CCAGTTTTGA
TTTAAACGTG
GCCAATATGG
ACAACTTCTT
CGCCCCCGTT
TTCACCATGG
GCAAATATTA
TACGCAAGGC
GACAAGGTGC
TGATGCCGCT
GGCGATTCAG
GTTCATCATG
CCGTTTGTGA
TGGCTTCCAT
GTCGGCAGAA
TGCTTAATGA
ATTACAACAG
TACTGCGATG
AGTGGCAGGC
GGGGCGTAAT
CTAGAGGATC
CGGCTTACTA
AAAGCCAGAT
AACAGTATGC
GTATTTGCGC
GCTGATTTTT
GCGGTATAAG
AATATATACT
GATATGTATA
CCCGAAGTAT
GTCAAAAAGA
GGTATGCTAT
GAAGCAGCGT
ATTACAGTGA
CAGTTGACAG
CGACAGCTAT
CAGTTGCTCA
AGGCATATAT
GATGTCAATA
TCTCCGGTCT
GGTAAGCACA
ACCATGCAGA
ATGAAGCCCG
TCGTCTGCGT
GCCGAACGCT
GGAAAGCGGA
AAATCAGGAA
GGGATGGCTG
AGGTCGCCCG
GTTTATTGAA
ATGAACGGCT
CTTTTGCTGA
CGAGAACAGG
GGCTGGTGAA
ATGCAGTTTA
AGGTTTACAC
CTATAAAAGA
GAGAGCCGTT
ATCGTCTGTT
TGTGGATGTA
CAGAGTGATA
TTATTGACAC
GCCCGGGCGA
CGGATGGTGA
TCCCCCTGGC
CAGTGCACGT
CTGCTGTCAG
ATAAAGTCCC
CCGTGAACTT
TACCCGGTGG
TGCATATCGG
GGATGAAAGC
TGGCGCATGA
TGACCACCGA
TATGGCCAGT
GTGCCGGTCT
CCGTTATCGG
GGAAGAAGTG
GCTGATCTCA
GCCACCGCGA
AAATGACATC
AAAAACGCCA
TTAACCTGAT
GTTCTGGGGA
ATATAAATGT
CAGGCTCCCT
TATACACAGC
CAGTCTGCAG
GTCGACCATA
GTGACTGGAT
ATGTTGTGTT
TTACAGTATT
ATGTAGTCTG
TTTTTTATGC
AAAATCTAAT
TTAATATATT
GATATTTATA
TCATTTTACG
TTTCTCGTTC
AGCTTTCTTG
TACAAAGTGG
TTTGATGGCC
GCTAAGTAAG
TAAGACGTCG
AGCTCTAAGT
AAGTAACGGC
CGCCACCGCG
GTGGAGCTTT
GGACTTCTTC
GCCAGAGGTT
TGGTCAAGTC
TCCAATCAAG
GTTGTCGGCT
TGTCTACCTT
GCCAGAAATT
TACGAAAAGA
TGGAAAAGGG
TCAAATCGTT
GGTAGATACG
TTGTTGACAC
TTCTAAATAA
GCGAATTTCT
TATGATTTAT
GATTTTTATT
ATTAAATAAG
TTATAAAAAA
AATAAGTGTA
TACAAATTTT
AAAGTGACTC
TTAGGTTTTA
AAACGAAAAT
TCTTATTCTT
GAGTAACTCT
TTCCTGTAGG
TCAGGTTGCT
TTCTCAGGTA
TAGCATGAGG
TCGCTCTTAT
TGACCACACC
TCTACCGGCA
TGCCGAGCAA
ATGCCTGCAA
ATCGCTCCCC
ATTTCACCCA
ATTGTAGATA
TGCTAACTCC
AGCAATGAGT
TGATGAATCT
CGGTGTGTAT
TTTATGTCCT
CAGAGGACAA
CACCTGTTGT
AATCGTTCTT
CCACACGGAT
CCCAATTCGC
CCTATAGTGA
GTCGTATTAC
AATTCACTGG
CCGTCGTTTT
ACAACGTCGT
GACTGGGAAA
ACCCTGGCGT
TACCCAACTT
AATCGCCTTG
CAGCACATCC
CCCTTTCGCC
AGCTGGCGTA
ATAGCGAAGA
GGCCCGCACC
GATCGCCCTT
CCCAACAGTT
GCGCAGCCTG
AATGGCGAAT
GGACGCGCCC
TGTAGCGGCG
CATTAAGCGC
GGCGGGTGTG
GTGGTTACGC
GCAGCGTGAC
CGCTACACTT
GCCAGCGCCC
TAGCGCCCGC
TCCTTTCGCT
TTCTTCCCTT
CCTTTCTCGC
CACGTTCGCC
GGCTTTCCCC
GTCAAGCTCT
AAATCGGGGG
CTCCCTTTAG
GGTTCCGATT
TAGTGCTTTA
CGGCACCTCG
ACCCCAAAAA
ACTTGATTAG
GGTGATGGTT
CACGTAGTGG
GCCATCGCCC
TGATAGACGG
TTTTTCGCCC
TTTGACGTTG
GAGTCCACGT
TCTTTAATAG
TGGACTCTTG
TTCCAAACTG
GAACAACACT
CAACCCTATC
TCGGTCTATT
CTTTTGATTT
ATAAGGGATT
TTGCCGATTT
CGGCCTATTG
GTTAAAAAAT
GAGCTGATTT
AACAAAAATT
TAACGCGAAT
TTTAACAAAA
TATTAACGCT
TACAATTTCC
TGATGCGGTA
TTTTCTCCTT
ACGCATCTGT
GCGGTATTTC
ACACCGCATA
TCGACCGGTC
GAGGAGAACT
TCTAGTATAT
CTACATACCT
AATATTATTG
CCTTATTAAA
AATGGAATCC
CAACAATTAC
ATCAAAATCC
ACATTCTCTT
CAAAATCAAT
TGTCCTGTAC
TTCCTTGTTC
ATGTGTGTTC
AAAAACGTTA
TATTTATAGG
ATAATTATAC
TCTATTTCTC
AACAAGTAAT
TGGTTGTTTG
GCCGAGCGGT
CTAAGGCGCC
TGATTCAAGA
AATATCTTGA
CCGCAGTTAA
CTGTGGGAAT
ACTCAGGTAT
CGTAAGATGC
AAGAGTTCGA
ATCTCTTAGC
AACCATTATT
TTTTTCCTCA
ACATAACGAG
AACACACAGG
GGCGCTATCG
CACAGAATCA
AATTCGATGA
CTGGAAATTT
TTTGTTAATT
TCAGAGGTCG
CCTGACGCAT
ATACCTTTTT
CAACTGAAAA
ATTGGGAGAA
AAAGGAAAGG
TGAGAGCGCC
GGAACCGGCT
TTTCATATAG
AATAGAGAAG
CGTTCATGAC
TAAATGCTTG
CATCACAATA
CTTGAAGTTG
ACAATATTAT
TTAAGGACCT
ATTGTTTTTT
CCAATAGGTG
GTTAGCAATC
GTCTTACTTT
CTAACTTTTC
TTACCTTTTA
CATTTCAGCA
ATATATATAT
ATATATTTCA
AGGATATACC
ATTCTAATGT
CTGCCCCTAA
GAAGATCGTC
GTTTTGCCAG
GTGACCACGT
TGGTCAAGAA
ATCACAGCCG
AAGCCATTAA
GGTTCTTAAA
GCTATTTCTG
ATGTTCGTTC
CAATGTCAAG
TTCGATTTCG
AAAATCATTT
AATTGGTGGT
GCTGCTATCG
ATGCTACAGG
TGTTCCACTT
CCAGATGAGG
CGCTGGAAGC
CTCCAAGAAG
GCTGATGCCG
TTTTGTTAGG
TGCTGTGGGT
GGTCCTAAAT
GGGGTACCGG
TAGTGTTAGA
CCTGAACAAG
GTTTACTAAA
AATCCGTAAA
GAACTTCAAT
TGTACGCCAA
CTTAAGACCA
TGTAACTTTG
CATCCGACTC
TCTTTTAGAC
TTATCTCCAA
TCAAGCCACA
ATTTGCTAAA
GGTACTGACT
TCGTTGTTGT
CAGAGAATTA
GTGGGAGGTA
TTTACTTTGG
TAAGAGAAAG
GAAGACGATG
GTGATGGTGT
CGCTTGGGAT
AGTGAACAAT
ACACCGTTCC
AGAAGTGCAA
AGAATCACAA
GAATGGCCGC
TTTCATGGCC
CTACAACATG
AGCCACCATT
GCCTATTTGG
TCCTTGGATA
AAGCTAATGT
TTTGGCCTCT
TCAAGATTAT
GGAGAAAAAC
TGTGGAGGAA
ACCATCAAGA
ACGAATTCCC
TACATTGAAG
GTTCAACATC
AATTGATTGA
TTCTGCCGCC
ATGATCCTAG
TTAAGAACCC
AACCCACCTA
AATGGTATTA
TAATCACCAG
CAACATGTTT
GGTGATATCA
TCTCCGATGA
AGCCTCCGTT
ATCCCAGGTT
CCTTGGGTTT
GTTGCCATCT
GCGTCCTTGG
CCTCTTTGCC
AGACAAGAAC
ACCGCATTTG
GTTTGTACGA
ACCATGCCAC
GGTTCTGCTC
CAGATTTGCC
AAAGAATAAG
GTCAACCCTA
TCGCCACTAT
CTTGTCTGCT
GCAATGATGT
TGAAATTGTC
ATTGAACTTG
CCTGAAGAAG
GTAAGGCCAT
TGAAGATGCA
GTTAAAAAGG
TTTTGGATGC
AGGTATCAGA
ACTGGTGATT
TAGGTGGTTC
CAACAGTACC
ACCGAAGTCG
GTGATGCTGT
CGCCGAAGAA
GTTAAGAAAA
TCCTTGCTTA
AAAAGATTCT
CTTTTTTTAT
GATATTTGTA
CATAAACTTT
ATAAATGAAA
TTCATAATAG
AAACGACACG
AAATTACAAA
ATGGAATATG
TTCATAGGGT
AGACGAAACT
ATATACGCAA
TCTACATACA
TTTATCAAGA
AGGAGAAAAA
GGAGGATGTA
AAGGAATACA
GGTAAGCAAA
TTGATACTAA
TGGCTCAACG
TGATAAGGAA
AAAGAATTGC
ACTTTAACAT
TAATATTGAC
AAGGAGGAGG
GCACCACACA
AAAAGTTAGG
TGTAACAGAA
AATCATGAAA
CTATGATTCC
TAATTTATAT
ATTGGAGGAT
TTTCTCTAAA
AAAAAAAAAA
TACAACAAAT
AAAAAACACT
CAATGACCTG
ACCATTTGAT
GGAGTTTAAG
TCAATACCTT
CTTGAACCAT
TTCCCATAAT
GGTGAAAGTT
CCCTCAAGAA
TTTTACTCTG
TCAGAAACGG
CCTTAACGAC
GTAGTCGATA
TGGTGCACTC
TCAGTACAAT
CTGCTCTGAT
GCCGCATAGT
TAAGCCAGCC
CCGACACCCG
CCAACACCCG
CTGACGCGCC
CTGACGGGCT
TGTCTGCTCC
CGGCATCCGC
TTACAGACAA
GCTGTGACCG
TCTCCGGGAG
CTGCATGTGT
CAGAGGTTTT
CACCGTCATC
ACCGAAACGC
GCGAGACGAA
AGGGCCTCGT
GATACGCCTA
TTTTTATAGG
TTAATGTCAT
GATAATAATG
GTTTCTTAGG
ACGGATCGCT
TGCCTGTAAC
TTACACGCGC
CTCGTATCTT
TTAATGATGG
AATAATTTGG
GAATTTACTC
TGTGTTTATT
TATTTTTATG
TTTTGTATTT
GGATTTTAGA
AAGTAAATAA
AGAAGGTAGA
AGAGTTACGG
AATGAAGAAA
AAAAAATAAA
CAAAGGTTTA
AAAAATTTCA
ACAAAAAGCG
TACTTTACAT
ATATATTTAT
TAGACAAGAA
AAGCAGATTA
AATAGATATA
CATTCGATTA
ACGATAAGTA
AAATGTAAAA
TCACAGGATT
TTCGTGTGTG
GTCTTCTACA
CAGACAAGAT
GAAACAATTC
GGCATTAATA
CCTGAGAGCA
GGAAGAGCAA
GATAAAAGGT
AGTATTTGTT
GGCGATCCCC
CTAGAGTCTT
TTACATCTTC
GGAAAACAAA
AACTATTTTT
TCTTTAATTT
CTTTTTTTAC
TTTCTATTTT
TAATTTATAT
ATTTATATTA
AAAAATTTAA
ATTATAATTA
TTTTTATAGC
ACGTGATGAA
AAGGACCCAG
GTGGCACTTT
TCGGGGAAAT
GTGCGCGGAA
CCCCTATTTG
TTTATTTTTC
TAAATACATT
CAAATATGTA
TCCGCTCATG
AGACAATAAC
CCTGATAAAT
GCTTCAATAA
TCTGCAGCTC
TGGCCCGTGT
CTCAAAATCT
CTGATGTTAC
ATTGCACAAG
ATAAAAATAT
ATCATCATGA
ACAATAAAAC
TGTCTGCTTA
CATAAACAGT
AATACAAGGG
GTGTTATGAG
CCATATTCAA
CGGGAAACGT
CGAGGCCGCG
ATTAAATTCC
AACATGGATG
CTGATTTATA
TGGGTATAAA
TGGGCTCGGG
CCGGGAAGCC
GATCTCGGCT
TGAACGAATT
GTTAGGTGGC
GGTACTTGGG
TCGATATCAA
AGTGCATCAC
TTCTTCCCGT
ATGCCCAACT
TTGTATAGAG
AGCCACTGCG
GGATCGTCAC
CGTAATCTGC
TTGCACGTAG
ATCACATAAG
CACCAAGCGC
GTTGGCCTCA
TGCTTGAGGA
GATTGATGAG
CGCGGTGGCA
ATGCCCTGCC
TCCGGTGCTC
GCCGGAGACT
GCGAGATCAT
AGATATAGAT
CTCACTACGC
GGCTGCTCAA
ACTTGGGCAG
AACGTAAGCC
GCGAGAGCGC
CAACAACCGC
TTCTTGGTCG
AAGGCAGCAA
GCGCGATGAA
TGTCTTACTA
CGGAGCAAGT
TCCCGAGGTA
ATCGGAGTCC
GGCTGATGTT
GGGAGTAGGT
GGCTACGTCT
CCGAACTCAC
GACCGAAAAG
ATCAAGAGCA
GCCCGCATGG
ATTTGACTTG
GTCAGGGCCG
AGCCTACATG
TGCGAATGAT
GCCCATACTT
GAGCCACCTA
ACTTTGTTTT
AGGGCGACTG
CCCTGCTGCG
TAACATCGTT
GCTGCTGCGT
AACATCGTTG
CTGCTCCATA
ACATCAAACA
TCGACCCACG
GCGTAACGCG
CTTGCTGCTT
GGATGCCCGA
GGCATAGACT
GTACAAAAAA
ACAGTCATAA
CAAGCCATGA
AAACCGCCAC
TGCGCCGTTA
CCACCGCTGC
GTTCGGTCAA
GGTTCTGGAC
CAGTTGCGTG
AGCGCATACG
CTACTTGCAT
TACAGTTTAC
GAACCGAACA
GGCTTATGTC
AACTGGGTTC
GTGCCTTCAT
CCGTTTCCAC
GGTGTGCGTC
ACCCGGCAAC
CTTGGGCAGC
AGCGAAGTCG
AGGCATTTCT
GTCCTGGCTG
GCGAACGAGC
GCAAGGTTTC
GGTCTCCACG
CATCGTCAGG
CATTGGCGGC
CTTGCTGTTC
TTCTACGGCA
AGGTGCTGTG
CACGGATCTG
CCCTGGCTTC
AGGAGATCGG
AAGACCTCGG
CCGTCGCGGC
GCTTGCCGGT
GGTGCTGACC
CCGGATGAAG
TGGTTCGCAT
CCTCGGTTTT
CTGGAAGGCG
AGCATCGTTT
GTTCGCCCAG
GACTCTAGCT
ATAGTTCTAG
TGGTTGGCTA
CTAATAGGTT
GTATTGATGT
TGGACGAGTC
GGAATCGCAG
ACCGATACCA
GGATCTTGCC
ATCCTATGGA
ACTGCCTCGG
TGAGTTTTCT
CCTTCATTAC
AGAAACGGCT
TTTTCAAAAA
TATGGTATTG
ATAATCCTGA
TATGAATAAA
TTGCAGTTTC
ATTTGATGCT
CGATGAGTTT
TTCTAATCAG
AATTGGTTAA
TTGGTTGTAA
CACTGGCAGA
GCATTACGCT
GACTTGACGG
GACGGCGCAA
GCTCATGACC
AAAATCCCTT
AACGTGAGTT
TTCGTTCCAC
TGAGCGTCAG
ACCCCGTAGA
AAAGATCAAA
GGATCTTCTT
GAGATCCTTT
TTTTCTGCGC
GTAATCTGCT
GCTTGCAAAC
AAAAAAACCA
CCGCTACCAG
CGGTGGTTTG
TTTGCCGGAT
CAAGAGCTAC
CAACTCTTTT
TCCGAAGGTA
ACTGGCTTCA
GCAGAGCGCA
GATACCAAAT
ACTGTTCTTC
TAGTGTAGCC
GTAGTTAGGC
CACCACTTCA
AGAACTCTGT
AGCACCGCCT
ACATACCTCG
CTCTGCTAAT
CCTGTTACCA
GTGGCTGCTG
CCAGTGGCGA
TAAGTCGTGT
CTTACCGGGT
TGGACTCAAG
ACGATAGTTA
CCGGATAAGG
CGCAGCGGTC
GGGCTGAACG
GGGGGTTCGT
GCACACAGCC
CAGCTTGGAG
CGAACGACCT
ACACCGAACT
GAGATACCTA
CAGCGTGAGC
TATGAGAAAG
CGCCACGCTT
CCCGAAGGGA
GAAAGGCGGA
CAGGTATCCG
GTAAGCGGCA
GGGTCGGAAC
AGGAGAGCGC
ACGAGGGAGC
TTCCAGGGGG
AAACGCCTGG
TATCTTTATA
GTCCTGTCGG
GTTTCGCCAC
CTCTGACTTG
AGCGTCGATT
TTTGTGATGC
TCGTCAGGGG
GGCGGAGCCT
ATGGAAAAAC
GCCAGCAACG
CGGCCTTTTT
ACGGTTCCTG
GCCTTTTGCT
GGCCTTTTGC
TCACATGTTC
TTTCCTGCGT
TATCCCCTGA
TTCTGTGGAT
AACCGTATTA
CCGCCTTTGA
GTGAGCTGAT
ACCGCTCGCC
GCAGCCGAAC
GACCGAGCGC
AGCGAGTCAG
TGAGCGAGGA
AGCGGAAGAG
CGCCCAATAC
GCAAACCGCC
TCTCCCCGCG
CGTTGGCCGA
TTCATTAATG
CAGCTGGCAC
GACAGGTTTC
CCGACTGGAA
AGCGGGCAGT
GAGCGCAACG
CAATTAATGT
GAGTTAGCTC
ACTCATTAGG
CACCCCAGGC
TTTACACTTT
ATGCTTCCGG
CTCGTATGTT
GTGTGGAATT
GTGAGCGGAT
AACAATTTCA
CACAGGAAAC
AGCTATGACC
ATGATTACGC
CAAGCTCGAA
ATTAACCCTC
ACTAAAGGGA
ACAAAAGCTG
GTACCGATCC
CGAGCTTGCA
AATTAAAGCC
TTCGAGCGTC
CCAAAACCTT
CTCAAGCAAG
GTTTTCAGTA
TAATGTTACA
TGCGTACACG
CGTCTGTACA
GAAAAAAAAG
AAAAATTTGA
AATATAAATA
ACGTTCTTAA
TACTAACATA
ACTATAAAAA
AATAAATAGG
GACCTAGACT
TCAGGTTGTC
TAACTCCTTC
CTTTTCGGTT
AGAGCGGATG
TGGGGGGAGG
GCGTGAATGT
AAGCGTGACA
TAACTAATTA
CATGATATCG
ACAAAGGAAA
AGGGGCCTGT
TTACTCACAG
GCTTTTTTCA
AGTAGGTAAT
TAAGTCGTTT
CTGTCTTTTT
CCTTCTTCAA
CCCACCAAAG
GCCATCTTGG
TACTTTTTTT
TTTTTTTTTT
TTTTTTTTTT
TTTTTTTTTT
TTTTTTTTTT
TTTTTTTTTT
TTTTTTTTTT
TTTTTTTTTT
TTTTGATAGA
AATAATACAG
AAGTAGATGT
TGAATTAGAT
TAAACTGAAG
ATATATAATT
TATTGGAAAA
TACATAGAGT
TTTTGTTGAT
GCGCTTAAGC
GATCAATTCA
ACAACACCAC
CAGCAGCTCT
GATTTTTTCT
TCAGCCAACT
TGGAGACGAA
TCTAGCTTTG
ACGATAACTG
GAACATTTGG
AATTCTACCC
TTACCCAAGA
TCTTACCGTA
ACCGGCTGCC
AAAGTGTCAA
TAACTGGAGC
AGTTTCCTTA
GAAGCAGATT
TCAAGTATTG
GTCTCTCTTG
TCTTCTGGGA
TCAATGTCCA
CAATTTGTCC
AAGTTCAAGA
CTGGCTTCCA
GAAATGAGCT
TGTTGCTTGT
GGAAGTATCT
CATACCAACC
TTACCGAAAT
AACCTGGATG
GTATTTATCC
ATGTTAATTC
TGTGGTGATG
TTGACCACCG
GCCATACCTC
TACCACCGGG
GTGCTTTCTG
TGCTTACCGA
TACGACCTTT
ACCGGCTGAG
ACGTGACCTC
TGTGCTTTCT
AGTCTTAGTG
AATCTGGAAG
GCATTCTTGA
TTAGTTGGAT
GATTGTTCTG
GGATTTAATG
CAAAAAAATC
ACTAAGAAGG
AAAAAAATCA
ACGGAGAAAG
CAAACGCCAT
CTTAAATATA
CGGGATACAG
ATGAAAGGTT
TGAACCTATC
TGGGAAAATA
CGCATTAAAC
AAGCGAAAAA
CTGCGAGGAA
AATTGTTTGC
GTCTCTGCGG
GCTATTCACG
CGCCAGAGGA
AAAATAGGAA
AAATAACAGG
CATTAGAAAA
ATAATTTTGA
TTTTGGTAAT
GTGTGGGTCC
CTGGTGTACA
GATGTTACAT
TGGTTACAGT
ACTCTTGTTT
TTGCTGTGTT
TTTCGATGAA
TCTCCAAAAT
GGTTGTTAGC
ACATGGAAGA
GTCACCGATG
CTAAGT