名称: | pCX-OKS-2A |
---|---|
别称: | Plasmid#19771 |
基因长: | 5600bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达,干细胞重编程 |
片段类型: | ORF,IPS |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | CAG |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTCGACATTG
ATTATTGACT
AGTTATTAAT
AGTAATCAAT
TACGGGGTCA
TTAGTTCATA
GCCCATATAT
GGAGTTCCGC
GTTACATAAC
TTACGGTAAA
TGGCCCGCCT
GGCTGACCGC
CCAACGACCC
CCGCCCATTG
ACGTCAATAA
TGACGTATGT
TCCCATAGTA
ACGCCAATAG
GGACTTTCCA
TTGACGTCAA
TGGGTGGAGT
ATTTACGGTA
AACTGCCCAC
TTGGCAGTAC
ATCAAGTGTA
TCATATGCCA
AGTACGCCCC
CTATTGACGT
CAATGACGGT
AAATGGCCCG
CCTGGCATTA
TGCCCAGTAC
ATGACCTTAT
GGGACTTTCC
TACTTGGCAG
TACATCTACG
TATTAGTCAT
CGCTATTACC
ATGGTCGAGG
TGAGCCCCAC
GTTCTGCTTC
ACTCTCCCCA
TCTCCCCCCC
CTCCCCACCC
CCAATTTTGT
ATTTATTTAT
TTTTTAATTA
TTTTGTGCAG
CGATGGGGGC
GGGGGGGGGG
GGGGGGCGCG
CGCCAGGCGG
GGCGGGGCGG
GGCGAGGGGC
GGGGCGGGCG
AGGCGGAGAG
GTGCGGCGGC
AGCCAATCAG
AGCGGCGCGC
TCCGAAAGTT
TCCTTTTATG
GCGAGGCGGC
GGCGGCGGCG
GCCCTATAAA
AAGCGAAGCG
CGCGGCGGGC
GGGAGTCGCT
GCGCGCTGCC
TTCGCCCCGT
GCCCCGCTCC
GCCGCCGCCT
CGCGCCGCCC
GCCCCGGCTC
TGACTGACCG
CGTTACTCCC
ACAGGTGAGC
GGGCGGGACG
GCCCTTCTCC
TCCGGGCTGT
AATTAGCGCT
TGGTTTAATG
ACGGCTTGTT
TCTTTTCTGT
GGCTGCGTGA
AAGCCTTGAG
GGGCTCCGGG
AGGGCCCTTT
GTGCGGGGGG
AGCGGCTCGG
GGGGTGCGTG
CGTGTGTGTG
TGCGTGGGGA
GCGCCGCGTG
CGGCTCCGCG
CTGCCCGGCG
GCTGTGAGCG
CTGCGGGCGC
GGCGCGGGGC
TTTGTGCGCT
CCGCAGTGTG
CGCGAGGGGA
GCGCGGCCGG
GGGCGGTGCC
CCGCGGTGCG
GGGGGGGCTG
CGAGGGGAAC
AAAGGCTGCG
TGCGGGGTGT
GTGCGTGGGG
GGGTGAGCAG
GGGGTGTGGG
CGCGTCGGTC
GGGCTGCAAC
CCCCCCTGCA
CCCCCCTCCC
CGAGTTGCTG
AGCACGGCCC
GGCTTCGGGT
GCGGGGCTCC
GTACGGGGCG
TGGCGCGGGG
CTCGCCGTGC
CGGGCGGGGG
GTGGCGGCAG
GTGGGGGTGC
CGGGCGGGGC
GGGGCCGCCT
CGGGCCGGGG
AGGGCTCGGG
GGAGGGGCGC
GGCGGCCCCC
GGAGCGCCGG
CGGCTGTCGA
GGCGCGGCGA
GCCGCAGCCA
TTGCCTTTTA
TGGTAATCGT
GCGAGAGGGC
GCAGGGACTT
CCTTTGTCCC
AAATCTGTGC
GGAGCCGAAA
TCTGGGAGGC
GCCGCCGCAC
CCCCTCTAGC
GGGCGCGGGG
CGAAGCGGTG
CGGCGCCGGC
AGGAAGGAAA
TGGGCGGGGA
GGGCCTTCGT
GCGTCGCCGC
GCCGCCGTCC
CCTTCTCCCT
CTCCAGCCTC
GGGGCTGTCC
GCGGGGGGAC
GGCTGCCTTC
GGGGGGGACG
GGGCAGGGCG
GGGTTCGGCT
TCTGGCGTGT
GACCGGCGGC
TCTAGAGCCT
CTGCTAACCA
TGTTCATGCC
TTCTTCTTTT
TCCTACAGCT
CCTGGGCAAC
GTGCTGGTTA
TTGTGCTGTC
TCATCATTTT
GGCAAAGAAT
TCGCCCTTAA
AAAGCAGGCT
CCACCTTCCC
CATGGCTGGA
CACCTGGCTT
CAGACTTCGC
CTTCTCACCC
CCACCAGGTG
GGGGTGATGG
GTCAGCAGGG
CTGGAGCCGG
GCTGGGTGGA
TTCTCGAACC
TGGCTAAGCT
TCCAAGGGCC
TCCAGGTGGG
CCTGGAATCG
GACCAGGCTC
AGAGGTATTG
GGGATCTCCC
CATGTCCGCC
CGCATACGAG
TTCTGCGGAG
GGATGGCATA
CTGTGGACCT
CAGGTTGGAC
TGGGCCTAGT
CCCCCAAGTT
GGCGTGGAGA
CTTTGCAGCC
TGAGGGCCAG
GCAGGAGCAC
GAGTGGAAAG
CAACTCAGAG
GGAACCTCCT
CTGAGCCCTG
TGCCGACCGC
CCCAATGCCG
TGAAGTTGGA
GAAGGTGGAA
CCAACTCCCG
AGGAGTCCCA
GGACATGAAA
GCCCTGCAGA
AGGAGCTAGA
ACAGTTTGCC
AAGCTGCTGA
AGCAGAAGAG
GATCACCTTG
GGGTACACCC
AGGCCGACGT
GGGGCTCACC
CTGGGCGTTC
TCTTTGGAAA
GGTGTTCAGC
CAGACCACCA
TCTGTCGCTT
CGAGGCCTTG
CAGCTCAGCC
TTAAGAACAT
GTGTAAGCTG
CGGCCCCTGC
TGGAGAAGTG
GGTGGAGGAA
GCCGACAACA
ATGAGAACCT
TCAGGAGATA
TGCAAATCGG
AGACCCTGGT
GCAGGCCCGG
AAGAGAAAGC
GAACTAGCAT
TGAGAACCGT
GTGAGGTGGA
GTCTGGAGAC
CATGTTTCTG
AAGTGCCCGA
AGCCCTCCCT
ACAGCAGATC
ACTCACATCG
CCAATCAGCT
TGGGCTAGAG
AAGGATGTGG
TTCGAGTATG
GTTCTGTAAC
CGGCGCCAGA
AGGGCAAAAG
ATCAAGTATT
GAGTATTCCC
AACGAGAAGA
GTATGAGGCT
ACAGGGACAC
CTTTCCCAGG
GGGGGCTGTA
TCCTTTCCTC
TGCCCCCAGG
TCCCCACTTT
GGCACCCCAG
GCTATGGAAG
CCCCCACTTC
ACCACACTCT
ACTCAGTCCC
TTTTCCTGAG
GGCGAGGCCT
TTCCCTCTGT
TCCCGTCACT
GCTCTGGGCT
CTCCCATGCA
TTCAAACGGG
GATCTCAAAA
TTGTCGCTCC
TGTCAAACAA
ACTCTTAACT
TTGATTTACT
CAAACTGGCT
GGGGATGTAG
AAAGCAATCC
AGGTCCACTC
ATGGCTGTCA
GCGACGCTCT
GCTCCCGTCC
TTCTCCACGT
TCGCGTCCGG
CCCGGCGGGA
AGGGAGAAGA
CACTGCGTCC
AGCAGGTGCC
CCGACTAACC
GTTGGCGTGA
GGAACTCTCT
CACATGAAGC
GACTTCCCCC
ACTTCCCGGC
CGCCCCTACG
ACCTGGCGGC
GACGGTGGCC
ACAGACCTGG
AGAGTGGCGG
AGCTGGTGCA
GCTTGCAGCA
GTAACAACCC
GGCCCTCCTA
GCCCGGAGGG
AGACCGAGGA
GTTCAACGAC
CTCCTGGACC
TAGACTTTAT
CCTTTCCAAC
TCGCTAACCC
ACCAGGAATC
GGTGGCCGCC
ACCGTGACCA
CCTCGGCGTC
AGCTTCATCC
TCGTCTTCCC
CAGCGAGCAG
CGGCCCTGCC
AGCGCGCCCT
CCACCTGCAG
CTTCAGCTAT
CCGATCCGGG
CCGGGGGTGA
CCCGGGCGTG
GCTGCCAGCA
ACACAGGTGG
AGGGCTCCTC
TACAGCCGAG
AATCTGCGCC
ACCTCCCACG
GCCCCCTTCA
ACCTGGCGGA
CATCAATGAC
GTGAGCCCCT
CGGGCGGCTT
CGTGGCTGAG
CTCCTGCGGC
CGGAGTTGGA
CCCAGTATAC
ATTCCGCCAC
AGCAGCCTCA
GCCGCCAGGT
GGCGGGCTGA
TGGGCAAGTT
TGTGCTGAAG
GCGTCTCTGA
CCACCCCTGG
CAGCGAGTAC
AGCAGCCCTT
CGGTCATCAG
TGTTAGCAAA
GGAAGCCCAG
ACGGCAGCCA
CCCCGTGGTA
GTGGCGCCCT
ACAGCGGTGG
CCCGCCGCGC
ATGTGCCCCA
AGATTAAGCA
AGAGGCGGTC
CCGTCCTGCA
CGGTCAGCCG
GTCCCTAGAG
GCCCATTTGA
GCGCTGGACC
CCAGCTCAGC
AACGGCCACC
GGCCCAACAC
ACACGACTTC
CCCCTGGGGC
GGCAGCTCCC
CACCAGGACT
ACCCCTACAC
TGAGTCCCGA
GGAACTGCTG
AACAGCAGGG
ACTGTCACCC
TGGCCTGCCT
CTTCCCCCAG
GATTCCATCC
CCATCCGGGG
CCCAACTACC
CTCCTTTCCT
GCCAGACCAG
ATGCAGTCAC
AAGTCCCCTC
TCTCCATTAT
CAAGAGCTCA
TGCCACCGGG
TTCCTGCCTG
CCAGAGGAGC
CCAAGCCAAA
GAGGGGAAGA
AGGTCGTGGC
CCCGGAAAAG
AACAGCCACC
CACACTTGTG
ACTATGCAGG
CTGTGGCAAA
ACCTATACCA
AGAGTTCTCA
TCTCAAGGCA
CACCTGCGAA
CTCACACAGG
CGAGAAACCT
TACCACTGTG
ACTGGGACGG
CTGTGGGTGG
AAATTCGCCC
GCTCCGATGA
ACTGACCAGG
CACTACCGCA
AACACACAGG
GCACCGGCCC
TTTCAGTGCC
AGAAGTGCGA
CAGGGCCTTT
TCCAGGTCGG
ACCACCTTGC
CTTACACATG
AAGAGGCACT
TTGGGGATCT
CAAAATTGTC
GCTCCTGTCA
AACAAACTCT
TAACTTTGAT
TTACTCAAAC
TGGCTGGGGA
TGTAGAAAGC
AATCCAGGTC
CACTCATGAA
CAATTCGCCC
TTCACCATGT
ATAACATGAT
GGAGACGGAG
CTGAAGCCGC
CGGGCCCGCA
GCAAGCTTCG
GGGGGCGGCG
GCGGAGGAGG
CAACGCCACG
GCGGCGGCGA
CCGGCGGCAA
CCAGAAGAAC
AGCCCGGACC
GCGTCAAGAG
GCCCATGAAC
GCCTTCATGG
TATGGTCCCG
GGGGCAGCGG
CGTAAGATGG
CCCAGGAGAA
CCCCAAGATG
CACAACTCGG
AGATCAGCAA
GCGCCTGGGC
GCGGAGTGGA
AACTTTTGTC
CGAGACCGAG
AAGCGGCCGT
TCATCGACGA
GGCCAAGCGG
CTGCGCGCTC
TGCACATGAA
GGAGCACCCG
GATTATAAAT
ACCGGCCGCG
GCGGAAAACC
AAGACGCTCA
TGAAGAAGGA
TAAGTACACG
CTTCCCGGAG
GCTTGCTGGC
CCCCGGCGGG
AACAGCATGG
CGAGCGGGGT
TGGGGTGGGC
GCCGGCCTGG
GTGCGGGCGT
GAACCAGCGC
ATGGACAGCT
ACGCGCACAT
GAACGGCTGG
AGCAACGGCA
GCTACAGCAT
GATGCAGGAG
CAGCTGGGCT
ACCCGCAGCA
CCCGGGCCTC
AACGCTCACG
GCGCGGCACA
GATGCAACCG
ATGCACCGCT
ACGACGTCAG
CGCCCTGCAG
TACAACTCCA
TGACCAGCTC
GCAGACCTAC
ATGAACGGCT
CGCCCACCTA
CAGCATGTCC
TACTCGCAGC
AGGGCACCCC
CGGTATGGCG
CTGGGCTCCA
TGGGCTCTGT
GGTCAAGTCC
GAGGCCAGCT
CCAGCCCCCC
CGTGGTTACC
TCTTCCTCCC
ACTCCAGGGC
GCCCTGCCAG
GCCGGGGACC
TCCGGGACAT
GATCAGCATG
TACCTCCCCG
GCGCCGAGGT
GCCGGAGCCC
GCTGCGCCCA
GTAGACTGCA
CATGGCCCAG
CACTACCAGA
GCGGCCCGGT
GCCCGGCACG
GCCATTAACG
GCACACTGCC
CCTGTCGCAC
ATGTGAGGGC
TGGAAGGGCG
AATTGCCTGC
AGGAATTCAC
TCCTCAGGTG
CAGGCTGCCT
ATCAGAAGGT
GGTGGCTGGT
GTGGCCAATG
CCCTGGCTCA
CAAATACCAC
TGAGATCTTT
TTCCCTCTGC
CAAAAATTAT
GGGGACATCA
TGAAGCCCCT
TGAGCATCTG
ACTTCTGGCT
AATAAAGGAA
ATTTATTTTC
ATTGCAATAG
TGTGTTGGAA
TTTTTTGTGT
CTCTCACTCG
GAAGGACATA
TGGGAGGGCA
AATCATTTAA
AACATCAGAA
TGAGTATTTG
GTTTAGAGTT
TGGCAACATA
TGCCCATATG
CTGGCTGCCA
TGAACAAAGG
TTGGCTATAA
AGAGGTCATC
AGTATATGAA
ACAGCCCCCT
GCTGTCCATT
CCTTATTCCA
TAGAAAAGCC
TTGACTTGAG
GTTAGATTTT
TTTTATATTT
TGTTTTGTGT
TATTTTTTTC
TTTAACATCC
CTAAAATTTT
CCTTACATGT
TTTACTAGCC
AGATTTTTCC
TCCTCTCCTG
ACTACTCCCA
GTCATAGCTG
TCCCTCTTCT
CTTATGGAGA
TCCCTCGACC
TGCAGCCCAA
GCTTGGCGTA
ATCATGGTCA
TAGCTGTTTC
CTGTGTGAAA
TTGTTATCCG
CTCACAATTC
CACACAACAT
ACGAGCCGGA
AGCATAAAGT
GTAAAGCCTG
GGGTGCCTAA
TGAGTGAGCT
AACTCACATT
AATTGCGTTG
CGCTCACTGC
CCGCTTTCCA
GTCGGGAAAC
CTGTCGTGCC
AGCGGATCCG
CATCTCAATT
AGTCAGCAAC
CATAGTCCCG
CCCCTAACTC
CGCCCATCCC
GCCCCTAACT
CCGCCCAGTT
CCGCCCATTC
TCCGCCCCAT
GGCTGACTAA
TTTTTTTTAT
TTATGCAGAG
GCCGAGGCCG
CCTCGGCCTC
TGAGCTATTC
CAGAAGTAGT
GAGGAGGCTT
TTTTGGAGGC
CTAGGCTTTT
GCAAAAAGCT
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GGATCCGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTGG