名称: | pBAV1k-T5-LUX |
---|---|
别称: | Plasmid#55800;LuxCDABE |
基因长: | |
质粒宿主: | 大肠杆菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | |
原核抗性: | Kan |
筛选标记: | |
荧光标记: | LuX |
启动子: | T5 |
复制子: | |
拷贝数: | |
感受态: | DH5a |
温度: | 30度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: | 组成型 |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AATTCGCGGC
CGCTTCTAGA
GGAAATCATA
AAAAATTTAT
TTGCTTTGTG
AGCGGATAAC
AATTATAATA
GATTCAATTG
TGAGCGGATA
ACAATTACTA
GAGAAAGAGG
AGAAATACTA
GATGAAATTT
GGAAACTTTT
TGCTTACATA
CCAACCTCCC
CAATTTTCTC
AAACAGAGGT
AATGAAACGT
TTGGTTAAAT
TAGGTCGCAT
CTCTGAGGAG
TGTGGTTTTG
ATACCGTATG
GTTACTGGAG
CATCATTTCA
CGGAGTTTGG
TTTGCTTGGT
AACCCTTATG
TCGCTGCTGC
ATATTTACTT
GGCGCGACTA
AAAAATTGAA
TGTAGGAACT
GCCGCTATTG
TTCTTCCCAC
AGCCCATCCA
GTACGCCAAC
TTGAAGATGT
GAATTTATTG
GATCAAATGT
CAAAAGGACG
ATTTCGGTTT
GGTATTTGCC
GAGGGCTTTA
CAACAAGGAC
TTTCGCGTAT
TCGGCACAGA
TATGAATAAC
AGTCGCGCCT
TAGCGGAATG
CTGGTACGGG
CTGATAAAGA
ATGGCATGAC
AGAGGGATAT
ATGGAAGCTG
ATAATGAACA
TATCAAGTTC
CATAAGGTAA
AAGTAAACCC
CGCGGCGTAT
AGCAGAGGTG
GCGCACCGGT
TTATGTGGTG
GCTGAATCAG
CTTCGACGAC
TGAGTGGGCT
GCTCAATTTG
GCCTACCGAT
GATATTAAGT
TGGATTATAA
ATACTAACGA
AAAGAAAGCA
CAACTTGAGC
TTTATAATGA
AGTGGCTCAA
GAATATGGGC
ACGATATTCA
TAATATCGAC
CATTGCTTAT
CATATATAAC
ATCTGTAGAT
CATGACTCAA
TTAAAGCGAA
AGAGATTTGC
CGGAAATTTC
TGGGGCATTG
GTATGATTCT
TATGTGAATG
CTACGACTAT
TTTTGATGAT
TCAGACCAAA
CAAGAGGTTA
TGATTTCAAT
AAAGGGCAGT
GGCGTGACTT
TGTATTAAAA
GGACATAAAG
ATACTAATCG
CCGTATTGAT
TACAGTTACG
AAATCAATCC
CGTGGGAACG
CCGCAGGAAT
GTATTGACAT
AATTCAAAAA
GACATTGATG
CTACAGGAAT
ATCAAATATT
TGTTGTGGAT
TTGAAGCTAA
TGGAACAGTA
GACGAAATTA
TTGCTTCCAT
GAAGCTCTTC
CAGTCTGATG
TCATGCCATT
TCTTAAAGAA
AAACAACGTT
CGCTATTATA
TTAATAATAC
TAGAGAAAGA
GGAGAAATAC
TAGATGAAAT
TTGGATTGTT
CTTCCTTAAC
TTCATCAATT
CAACAACTGT
TCAAGAACAA
AGTATAGTTC
GCATGCAGGA
AATAACGGAG
TATGTTGATA
AGTTGAATTT
TGAACAGATT
TTAGTGTATG
AAAATCATTT
TTCAGATAAT
GGTGTTGTCG
GCGCTCCTCT
GACTGTTTCT
GGTTTTCTGC
TCGGTTTAAC
AGAGAAAATT
AAAATTGGTT
CATTAAATCA
CATCATTACA
ACTCATCATC
CTGTCCGCAT
AGCGGAGGAA
GCTTGCTTAT
TGGATCAGTT
AAGTGAAGGG
AGATTTATTT
TAGGGTTTAG
TGATTGCGAA
AAAAAAGATG
AAATGCATTT
TTTTAATCGC
CCGGTTGAAT
ATCAACAGCA
ACTATTTGAA
GAGTGTTATG
AAATCATTAA
CGATGCTTTA
ACAACAGGCT
ATTGTAATCC
AGATAACGAT
TTTTATAGCT
TCCCTAAAAT
ATCTGTAAAT
CCCCATGCTT
ATACGCCAGG
CGGACCTCGG
AAATATGTAA
CAGCAACCAG
TCATCATATT
GTTGAGTGGG
CGGCCAAAAA
AGGTATTCCT
CTCATCTTTA
AGTGGGATGA
TTCTAATGAT
GTTAGATATG
AATATGCTGA
AAGATATAAA
GCCGTTGCGG
ATAAATATGA
CGTTGACCTA
TCAGAGATAG
ACCATCAGTT
AATGATATTA
GTTAACTATA
ACGAAGATAG
TAATAAAGCT
AAACAAGAGA
CGCGTGCATT
TATTAGTGAT
TATGTTCTTG
AAATGCACCC
TAATGAAAAT
TTCGAAAATA
AACTTGAAGA
AATAATTGCA
GAAAACGCTG
TCGGAAATTA
TACGGAGTGT
ATAACTGCGG
CTAAGTTGGC
AATTGAAAAG
TGTGGTGCGA
AAAGTGTATT
GCTGTCCTTT
GAACCAATGA
ATGATTTGAT
GAGCCAAAAA
AATGTAATCA
ATATTGTTGA
TGATAATATT
AAGAAGTACC
ACATGGAATA
TACCTAATAA
TACTAGAGAA
AGAGGAGAAA
TACTAGATGA
CTAAAAAAAT
TTCATTCATT
ATTAACGGCC
AGGTTGAAAT
CTTTCCCGAA
AGTGATGATT
TAGTGCAATC
CATTAATTTT
GGTGATAATA
GTGTTTACCT
GCCAATATTG
AATGACTCTC
ATGTAAAAAA
CATTATTGAT
TGTAATGGAA
ATAACGAATT
ACGGTTGCAT
AACATTGTCA
ATTTTCTCTA
TACGGTAGGG
CAAAGATGGA
AAAATGAAGA
ATACTCAAGA
CGCAGGACAT
ACATTCGTGA
CTTAAAAAAA
TATATGGGAT
ATTCAGAAGA
AATGGCTAAG
CTAGAGGCCA
ATTGGATATC
TATGATTTTA
TGTTCTAAAG
GCGGCCTTTA
TGATGTTGTA
GAAAATGAAC
TTGGTTCTCG
CCATATCATG
GATGAATGGC
TACCTCAGGA
TGAAAGTTAT
GTTCGGGCTT
TTCCGAAAGG
TAAATCTGTA
CATCTGTTGG
CAGGTAATGT
TCCATTATCT
GGGATCATGT
CTATATTACG
CGCAATTTTA
ACTAAGAATC
AGTGTATTAT
AAAAACATCG
TCAACCGATC
CTTTTACCGC
TAATGCATTA
GCGTTAAGTT
TTATTGATGT
AGACCCTAAT
CATCCGATAA
CGCGCTCTTT
ATCTGTTATA
TATTGGCCCC
ACCAAGGTGA
TACATCACTC
GCAAAAGAAA
TTATGCGACA
TGCGGATGTT
ATTGTCGCTT
GGGGAGGGCC
AGATGCGATT
AATTGGGCGG
TAGAGCATGC
GCCATCTTAT
GCTGATGTGA
TTAAATTTGG
TTCTAAAAAG
AGTCTTTGCA
TTATCGATAA
TCCTGTTGAT
TTGACGTCCG
CAGCGACAGG
TGCGGCTCAT
GATGTTTGTT
TTTACGATCA
GCGAGCTTGT
TTTTCTGCCC
AAAACATATA
TTACATGGGA
AATCATTATG
AGGAATTTAA
GTTAGCGTTG
ATAGAAAAAC
TTAATCTATA
TGCGCATATA
TTACCGAATG
CCAAAAAAGA
TTTTGATGAA
AAGGCGGCCT
ATTCTTTAGT
TCAAAAAGAA
AGCTTGTTTG
CTGGATTAAA
AGTAGAGGTG
GATATTCATC
AACGTTGGAT
GATTATTGAG
TCAAATGCAG
GTGTGGAATT
TAATCAACCA
CTTGGCAGAT
GTGTGTACCT
TCATCACGTC
GATAATATTG
AGCAAATATT
GCCTTATGTT
CAAAAAAATA
AGACGCAAAC
CATATCTATT
TTTCCTTGGG
AGTCATCATT
TAAATATCGA
GATGCGTTAG
CATTAAAAGG
TGCGGAAAGG
ATTGTAGAAG
CAGGAATGAA
TAACATATTT
CGAGTTGGTG
GATCTCATGA
CGGAATGAGA
CCGTTGCAAC
GATTAGTGAC
ATATATTTCT
CATGAAAGGC
CATCTAACTA
TACGGCTAAG
GATGTTGCGG
TTGAAATAGA
ACAGACTCGA
TTCCTGGAAG
AAGATAAGTT
CCTTGTATTT
GTCCCATAAT
AATACTAGAG
AAAGAGGAGA
AATACTAGAT
GGAAAATGAA
TCAAAATATA
AAACCATCGA
CCACGTTATT
TGTGTTGAAG
GAAATAAAAA
AATTCATGTT
TGGGAAACGC
TGCCAGAAGA
AAACAGCCCA
AAGAGAAAGA
ATGCCATTAT
TATTGCGTCT
GGTTTTGCCC
GCAGGATGGA
TCATTTTGCT
GGTCTGGCGG
AATATTTATC
GCGGAATGGA
TTTCATGTGA
TCCGCTATGA
TTCGCTTCAC
CACGTTGGAT
TGAGTTCAGG
GACAATTGAT
GAATTTACAA
TGTCTATAGG
AAAGCAGAGC
TTGTTAGCAG
TGGTTGATTG
GTTAACTACA
CGAAAAATAA
ATAACTTCGG
TATGTTGGCT
TCAAGCTTAT
CTGCGCGGAT
AGCTTATGCA
AGCCTATCTG
AAATCAATGC
TTCGTTTTTA
ATCACCGCAG
TCGGTGTTGT
TAACTTAAGA
TATTCTCTTG
AAAGAGCTTT
AGGGTTTGAT
TATCTCAGTC
TACCCATTAA
TGAATTGCCG
GATAATCTGG
ATTTTGAAGG
CCATAAATTG
GGTGCTGAAG
TCTTTGCGAG
AGATTGTCTT
GATTTTGGTT
GGGAAGATTT
AGCTTCTACA
ATTAATAACA
TGATGTATCT
TGATATACCG
TTTATTGCTT
TTACTGCAAA
TAACGATAAT
TGGGTCAAGC
AAGATGAAGT
TATCACATTG
TTATCAAATA
TTCGTAGTAA
TCGATGCAAG
ATATATTCTT
TGTTAGGAAG
TTCGCATGAC
TTGAGTGAAA
ATTTAGTGGT
CCTGCGCAAT
TTTTATCAAT
CGGTTACGAA
AGCCGCTATC
GCGATGGATA
ATGATCATCT
GGATATTGAT
GTTGATATTA
CTGAACCGTC
ATTTGAACAT
TTAACTATTG
CGACAGTCAA
TGAACGCCGA
ATGAGAATTG
AGATTGAAAA
TCAAGCAATT
TCTCTGTCTT
AATAATACTA
GAGAAAGAGG
AGAAATACTA
GATGACTTCA
TATGTTGATA
AACAAGAAAT
TACAGCAAGC
TCAGAAATTG
ATGATTTGAT
TTTTTCGAGC
GATCCATTAG
TGTGGTCTTA
CGACGAGCAG
GAAAAAATCA
GAAAGAAACT
TGTGCTTGAT
GCATTTCGTA
ATCATTATAA
ACATTGTCGA
GAATATCGTC
ACTACTGTCA
GGCACACAAA
GTAGATGACA
ATATTACGGA
AATTGATGAC
ATACCTGTAT
TCCCAACATC
GGTTTTTAAG
TTTACTCGCT
TATTAACTTC
TCAGGAAAAC
GAGATTGAAA
GTTGGTTTAC
CAGTAGCGGC
ACGAATGGTT
TAAAAAGTCA
GGTGGCGCGT
GACAGATTAA
GTATTGAGAG
ACTCTTAGGC
TCTGTGAGTT
ATGGCATGAA
ATATGTTGGT
AGTTGGTTTG
ATCATCAAAT
AGAATTAGTC
AATTTGGGAC
CAGATAGATT
TAATGCTCAT
AATATTTGGT
TTAAATATGT
TATGAGTTTG
GTGGAATTGT
TATATCCTAC
GACATTTACC
GTAACAGAAG
AACGAATAGA
TTTTGTTAAA
ACATTGAATA
GTCTTGAACG
AATAAAAAAT
CAAGGGAAAG
ATCTTTGTCT
TATTGGTTCG
CCATACTTTA
TTTATTTACT
CTGCCATTAT
ATGAAAGATA
AAAAAATCTC
ATTTTCTGGA
GATAAAAGCC
TTTATATCAT
AACCGGAGGC
GGCTGGAAAA
GTTACGAAAA
AGAATCTCTG
AAACGTGATG
ATTTCAATCA
TCTTTTATTT
GATACTTTCA
ATCTCAGTGA
TATTAGTCAG
ATCCGAGATA
TATTTAATCA
AGTTGAACTC
AACACTTGTT
TCTTTGAGGA
TGAAATGCAG
CGTAAACATG
TTCCGCCGTG
GGTATATGCG
CGAGCGCTTG
ATCCTGAAAC
GTTGAAACCT
GTACCTGATG
GAACGCCGGG
GTTGATGAGT
TATATGGATG
CGTCAGCAAC
CAGTTATCCA
GCATTTATTG
TTACCGATGA
TGTCGGGATA
ATTAGCAGAG
AATATGGTAA
GTATCCCGGC
GTGCTCGTTG
AAATTTTACG
TCGCGTCAAT
ACGAGGACGC
AGAAAGGGTG
TGCTTTAAGC
TTAACCGAAG
CGTTTGATAG
TTAATAATAC
TAGTAGCGGC
CGCTGCAGGC
CTCAGGGCCC
GATCGATGCC
GCCGCTTAAT
TAATTAATCC
AGAGGCATCA
AATAAAACGA
AAGGCTCAGT
CGAAAGACTG
GGCCTTTCGT
TTTATCTGTT
GTTTGTCGGT
GAACGCTCTC
CTGAGTAGGA
CAAATCCGCC
GCCCTAGACC
TAGTGTCATT
TTATTTCCCC
CGTTTCAGCA
TCAAGAACCT
TTGCATAACT
TGCTCTATAT
CCACACTGAT
AATTGCCCTC
AAACCATAAT
CTAAAGGCGC
TAGAGTTTGT
TGAAACAATA
TCTTTTACAT
CATTCGTATT
TAAAATTCCA
AACTCCGCTC
CCCTAAGGCG
AATAAAAGCC
ATTAAATCTT
TTGTATTTAC
CAAATTATAG
TCATCCACTA
TATCTAAGAG
TAAATTCTTC
AATTCTCTTT
TTTGGCTTTC
ATCAAGTGTT
ATATAGCGGT
CAATATCAAA
ATCATTAATG
TTCAAAATAT
CTTTTTTGTC
GTATATATGT
TTATTCTTAG
CAATAGCGTC
CTTTGATTCA
TGAGTCAAAT
ATTCATATGA
ACCTTTGATA
TAATCAAGTA
TCTCAACATG
AGCAACTGAA
CTATTCCCCA
ATTTTCGCTT
AATCTTGTTC
CTAACGCTTT
CTATTGTTAC
AGGATTTCGT
GCAATATATA
TAACGTGATA
GTGTGGTTTT
TTATAGTGCT
TTCCATTTCG
TATAACATCA
CTACTATTCC
ATGTATCTTT
ATCTTTTTTT
TCGTCCATAT
CGTGTAAAGG
ACTGACAGCC
ATAGATACGC
CCAAACTCTC
TAATTTTTCC
TTCCAATCAT
TAGGAATTGA
GTCAGGATAT
AATAAAAATC
CAAAATTTCT
AGCTTTAGTA
TTTTTAATAG
CCATGATATA
ATTACCTTAT
CAAAAACAAG
TAGCGAAAAC
TCGTATCCTT
CTAAAAACGC
GAGCTTTCGC
TTATTTTTTT
TGTTCTGATT
CCTTTCTTGC
ATATTCTTCT
ATAGCTAACG
CCGCAACCGC
AGATTTTGAA
AAACCTTTTT
GTTTCGCCAT
ATCTGTTAAT
TTTTTATCTT
GCTCTTTTGT
CAGAGAAATC
ATAACTCTTT
TTTTCGATTC
TGAAATCACC
ATTTAAAAAA
CTCCAATCAA
ATAATTTTAT
AAAGTTAGTG
TATCACTTTG
TAATCATAAA
AACAACAATA
AAGCTACTTA
AATATAGATT
TATAAAAAAC
GTTGGCGAAA
ACGTTGGCGA
TTCGTTGGCG
ATTGAAAAAC
CCCTTAAACC
CTTGAGCCAG
TTGGGATAGA
GCGTTTTTGG
CACAAAAATT
GGCACTCGGC
ACTTAATGGG
GGGTCGTAGT
ACGGAAGCAA
AATTCGCTTC
CTTTCCCCCC
ATTTTTTTCC
AAATTCCAAA
TTTTTTTCAA
AAATTTTCCA
GCGCTACCGC
TCGGCAAAAT
TGCAAGCAAT
TTTTAAAATC
AAACCCATGA
GGGAATTTCA
TTCCCTCATA
CTCCCTTGAG
CCTCCTCCAA
CCGAAATAGA
AGGGCGCTGC
GCTTATTATT
TCATTCAGTC
ATCGGCTTTC
ATAATCTAAC
AGACAACATC
TTCGCTGCAA
AGCCACGCTA
CGCTCAAGGG
CTTTTACGCT
ACGATAACGC
CTGTTTTAAC
GATTATGCCG
ATAACTAAAC
GAAATAAACG
CTAAAACGTC
TCAGAAACGA
TTTTGAGACG
TTTTAATAAA
AAATCGCCTA
GTGCTTGGAT
TCTCACCAAT
AAAAAACGCC
CGGCGGCAAC
CGAGCGTTCT
GAACAAATCC
AGATGGAGTT
CTGAGGTCAT
TACTGGATCT
ATCAACAGGA
GTCCAAGCGA
GCTCGGTACT
AAAACAATTC
ATCCAGTAAA
ATATAATATT
TTATTTTCTC
CCAATCAGGC
TTGATCCCCA
GTAAGTCAAA
AAATAGCTCG
ACATACTGTT
CTTCCCCGAT
ATCCTCCCTG
ATCGACCGGA
CGCAGAAGGC
AATGTCATAC
CACTTGTCCG
CCCTGCCGCT
TCTCCCAAGA
TCAATAAAGC
CACTTACTTT
GCCATCTTTC
ACAAAGATGT
TGCTGTCTCC
CAGGTCGCCG
TGGGAAAAGA
CAAGTTCCTC
TTCGGGCTTT
TCCGTCTTTA
AAAAATCATA
CAGCTCGCGC
GGATCTTTAA
ATGGAGTGTC
TTCTTCCCAG
TTTTCGCAAT
CCACATCGGC
CAGATCGTTA
TTCAGTAAGT
AATCCAATTC
GGCTAAGCGG
CCGTCTAAGC
TATTCGTATA
GGGACAATCC
GATATGTCGA
TGGAGTGAAA
GAGCCTGATG
CACTCCGCAT
ACAGCTCGAT
AGTCTTTTCA
GGGCTTTGTT
CATCTTCATA
CCCTTCCGAG
CAAAGGACGC
CATCGGCCTC
ACTCATGAGC
AGATTGCTCC
AGCCATCATG
CCGTTCAAAG
TGCAGGACCT
TTGGAACAGG
CAGCTTTCCT
TCCAGCCATA
GCATCATGTC
CTTTTCCCGT
TCCACATCAT
AGGTGGTCCC
TTTATACCGG
CTGTCCGTCA
TTTTTAAATA
TAGGATTTCA
TTTTCTCCCA
CCAGCTTATA
TACCTTAGCA
GGAGACATTC
CTTCCGTATC
TTTTACGCAG
CGGTATTCTT
CGATCAGTTT
TTTCAATTCC
GGTGATATTC
TCATTTTAGC
CATTTATTAT
TTCCTTCCTC
TTTTCTACAG
TATTTAAAGA
TACCCCAAGA
AGCTAATTAT
AACAAGACGA
ACTCCAATTC
ACTGTTCCTT
GCATTCTAAA
ACCTTAAATA
CAGAAAACAG
CCTTTTCAAA
GTTGTTTTCA
AAGTTGGCGT
ATAACATAGT
ATCGACGGAG
CCGATTTTGA
AACCACAATT
ATGATAGAAT
TTGACGTCG