产品货号：P0887
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | peSpCas9-Puro |
|---|---|
| 别称: | eSpCas9(1.1)-Puro |
| 基因长: | |
| 质粒宿主： | 哺乳细胞 |
| 质粒用途： | 基因编辑 |
| 片段类型： | CRISPR |
| 片段物种： | |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | |
| 启动子: | |
| 复制子: | |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | |
| 正向引物: | |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
GAGGGCCTAT
TTCCCATGAT
TCCTTCATAT
TTGCATATAC
GATACAAGGC
TGTTAGAGAG
ATAATTGGAA
TTAATTTGAC
TGTAAACACA
AAGATATTAG
TACAAAATAC
GTGACGTAGA
AAGTAATAAT
TTCTTGGGTA
GTTTGCAGTT
TTAAAATTAT
GTTTTAAAAT
GGACTATCAT
ATGCTTACCG
TAACTTGAAA
GTATTTCGAT
TTCTTGGCTT
TATATATCTT
GTGGAAAGGA
CGAAACACCG
GGTCTTCGAG
AAGACCTGTT
TTAGAGCTAG
AAATAGCAAG
TTAAAATAAG
GCTAGTCCGT
TATCAACTTG
AAAAAGTGGC
ACCGAGTCGG
TGCTTTTTTG
TTTTAGAGCT
AGAAATAGCA
AGTTAAAATA
AGGCTAGTCC
GTTTTTAGCG
CGTGCGCCAA
TTCTGCAGAC
AAATGGCTCT
AGAGGTACCC
GTTACATAAC
TTACGGTAAA
TGGCCCGCCT
GGCTGACCGC
CCAACGACCC
CCGCCCATTG
ACGTCAATAG
TAACGCCAAT
AGGGACTTTC
CATTGACGTC
AATGGGTGGA
GTATTTACGG
TAAACTGCCC
ACTTGGCAGT
ACATCAAGTG
TATCATATGC
CAAGTACGCC
CCCTATTGAC
GTCAATGACG
GTAAATGGCC
CGCCTGGCAT
TGTGCCCAGT
ACATGACCTT
ATGGGACTTT
CCTACTTGGC
AGTACATCTA
CGTATTAGTC
ATCGCTATTA
CCATGGTCGA
GGTGAGCCCC
ACGTTCTGCT
TCACTCTCCC
CATCTCCCCC
CCCTCCCCAC
CCCCAATTTT
GTATTTATTT
ATTTTTTAAT
TATTTTGTGC
AGCGATGGGG
GCGGGGGGGG
GGGGGGGGCG
CGCGCCAGGC
GGGGCGGGGC
GGGGCGAGGG
GCGGGGCGGG
GCGAGGCGGA
GAGGTGCGGC
GGCAGCCAAT
CAGAGCGGCG
CGCTCCGAAA
GTTTCCTTTT
ATGGCGAGGC
GGCGGCGGCG
GCGGCCCTAT
AAAAAGCGAA
GCGCGCGGCG
GGCGGGAGTC
GCTGCGACGC
TGCCTTCGCC
CCGTGCCCCG
CTCCGCCGCC
GCCTCGCGCC
GCCCGCCCCG
GCTCTGACTG
ACCGCGTTAC
TCCCACAGGT
GAGCGGGCGG
GACGGCCCTT
CTCCTCCGGG
CTGTAATTAG
CTGAGCAAGA
GGTAAGGGTT
TAAGGGATGG
TTGGTTGGTG
GGGTATTAAT
GTTTAATTAC
CTGGAGCACC
TGCCTGAAAT
CACTTTTTTT
CAGGTTGGAC
CGGTGCCACC
ATGGACTATA
AGGACCACGA
CGGAGACTAC
AAGGATCATG
ATATTGATTA
CAAAGACGAT
GACGATAAGA
TGGCCCCAAA
GAAGAAGCGG
AAGGTCGGTA
TCCACGGAGT
CCCAGCAGCC
GACAAGAAGT
ACAGCATCGG
CCTGGACATC
GGCACCAACT
CTGTGGGCTG
GGCCGTGATC
ACCGACGAGT
ACAAGGTGCC
CAGCAAGAAA
TTCAAGGTGC
TGGGCAACAC
CGACCGGCAC
AGCATCAAGA
AGAACCTGAT
CGGAGCCCTG
CTGTTCGACA
GCGGCGAAAC
AGCCGAGGCC
ACCCGGCTGA
AGAGAACCGC
CAGAAGAAGA
TACACCAGAC
GGAAGAACCG
GATCTGCTAT
CTGCAAGAGA
TCTTCAGCAA
CGAGATGGCC
AAGGTGGACG
ACAGCTTCTT
CCACAGACTG
GAAGAGTCCT
TCCTGGTGGA
AGAGGATAAG
AAGCACGAGC
GGCACCCCAT
CTTCGGCAAC
ATCGTGGACG
AGGTGGCCTA
CCACGAGAAG
TACCCCACCA
TCTACCACCT
GAGAAAGAAA
CTGGTGGACA
GCACCGACAA
GGCCGACCTG
CGGCTGATCT
ATCTGGCCCT
GGCCCACATG
ATCAAGTTCC
GGGGCCACTT
CCTGATCGAG
GGCGACCTGA
ACCCCGACAA
CAGCGACGTG
GACAAGCTGT
TCATCCAGCT
GGTGCAGACC
TACAACCAGC
TGTTCGAGGA
AAACCCCATC
AACGCCAGCG
GCGTGGACGC
CAAGGCCATC
CTGTCTGCCA
GACTGAGCAA
GAGCAGACGG
CTGGAAAATC
TGATCGCCCA
GCTGCCCGGC
GAGAAGAAGA
ATGGCCTGTT
CGGAAACCTG
ATTGCCCTGA
GCCTGGGCCT
GACCCCCAAC
TTCAAGAGCA
ACTTCGACCT
GGCCGAGGAT
GCCAAACTGC
AGCTGAGCAA
GGACACCTAC
GACGACGACC
TGGACAACCT
GCTGGCCCAG
ATCGGCGACC
AGTACGCCGA
CCTGTTTCTG
GCCGCCAAGA
ACCTGTCCGA
CGCCATCCTG
CTGAGCGACA
TCCTGAGAGT
GAACACCGAG
ATCACCAAGG
CCCCCCTGAG
CGCCTCTATG
ATCAAGAGAT
ACGACGAGCA
CCACCAGGAC
CTGACCCTGC
TGAAAGCTCT
CGTGCGGCAG
CAGCTGCCTG
AGAAGTACAA
AGAGATTTTC
TTCGACCAGA
GCAAGAACGG
CTACGCCGGC
TACATTGACG
GCGGAGCCAG
CCAGGAAGAG
TTCTACAAGT
TCATCAAGCC
CATCCTGGAA
AAGATGGACG
GCACCGAGGA
ACTGCTCGTG
AAGCTGAACA
GAGAGGACCT
GCTGCGGAAG
CAGCGGACCT
TCGACAACGG
CAGCATCCCC
CACCAGATCC
ACCTGGGAGA
GCTGCACGCC
ATTCTGCGGC
GGCAGGAAGA
TTTTTACCCA
TTCCTGAAGG
ACAACCGGGA
AAAGATCGAG
AAGATCCTGA
CCTTCCGCAT
CCCCTACTAC
GTGGGCCCTC
TGGCCAGGGG
AAACAGCAGA
TTCGCCTGGA
TGACCAGAAA
GAGCGAGGAA
ACCATCACCC
CCTGGAACTT
CGAGGAAGTG
GTGGACAAGG
GCGCTTCCGC
CCAGAGCTTC
ATCGAGCGGA
TGACCAACTT
CGATAAGAAC
CTGCCCAACG
AGAAGGTGCT
GCCCAAGCAC
AGCCTGCTGT
ACGAGTACTT
CACCGTGTAT
AACGAGCTGA
CCAAAGTGAA
ATACGTGACC
GAGGGAATGA
GAAAGCCCGC
CTTCCTGAGC
GGCGAGCAGA
AAAAGGCCAT
CGTGGACCTG
CTGTTCAAGA
CCAACCGGAA
AGTGACCGTG
AAGCAGCTGA
AAGAGGACTA
CTTCAAGAAA
ATCGAGTGCT
TCGACTCCGT
GGAAATCTCC
GGCGTGGAAG
ATCGGTTCAA
CGCCTCCCTG
GGCACATACC
ACGATCTGCT
GAAAATTATC
AAGGACAAGG
ACTTCCTGGA
CAATGAGGAA
AACGAGGACA
TTCTGGAAGA
TATCGTGCTG
ACCCTGACAC
TGTTTGAGGA
CAGAGAGATG
ATCGAGGAAC
GGCTGAAAAC
CTATGCCCAC
CTGTTCGACG
ACAAAGTGAT
GAAGCAGCTG
AAGCGGCGGA
GATACACCGG
CTGGGGCAGG
CTGAGCCGGA
AGCTGATCAA
CGGCATCCGG
GACAAGCAGT
CCGGCAAGAC
AATCCTGGAT
TTCCTGAAGT
CCGACGGCTT
CGCCAACAGA
AACTTCATGC
AGCTGATCCA
CGACGACAGC
CTGACCTTTA
AAGAGGACAT
CCAGAAAGCC
CAGGTGTCCG
GCCAGGGCGA
TAGCCTGCAC
GAGCACATTG
CCAATCTGGC
CGGCAGCCCC
GCCATTAAGA
AGGGCATCCT
GCAGACAGTG
AAGGTGGTGG
ACGAGCTCGT
GAAAGTGATG
GGCCGGCACA
AGCCCGAGAA
CATCGTGATC
GAAATGGCCA
GAGAGAACCA
GACCACCCAG
AAGGGACAGA
AGAACAGCCG
CGAGAGAATG
AAGCGGATCG
AAGAGGGCAT
CAAAGAGCTG
GGCAGCCAGA
TCCTGAAAGA
ACACCCCGTG
GAAAACACCC
AGCTGCAGAA
CGAGAAGCTG
TACCTGTACT
ACCTGCAGAA
TGGGCGGGAT
ATGTACGTGG
ACCAGGAACT
GGACATCAAC
CGGCTGTCCG
ACTACGATGT
GGACCATATC
GTGCCTCAGA
GCTTTCTGGC
CGACGACTCC
ATCGACAACA
AGGTGCTGAC
CAGAAGCGAC
AAGAACCGGG
GCAAGAGCGA
CAACGTGCCC
TCCGAAGAGG
TCGTGAAGAA
GATGAAGAAC
TACTGGCGGC
AGCTGCTGAA
CGCCAAGCTG
ATTACCCAGA
GAAAGTTCGA
CAATCTGACC
AAGGCCGAGA
GAGGCGGCCT
GAGCGAACTG
GATAAGGCCG
GCTTCATCAA
GAGACAGCTG
GTGGAAACCC
GGCAGATCAC
AAAGCACGTG
GCACAGATCC
TGGACTCCCG
GATGAACACT
AAGTACGACG
AGAATGACAA
GCTGATCCGG
GAAGTGAAAG
TGATCACCCT
GAAGTCCAAG
CTGGTGTCCG
ATTTCCGGAA
GGATTTCCAG
TTTTACAAAG
TGCGCGAGAT
CAACAACTAC
CACCACGCCC
ACGACGCCTA
CCTGAACGCC
GTCGTGGGAA
CCGCCCTGAT
CAAAAAGTAC
CCTGCGCTGG
AAAGCGAGTT
CGTGTACGGC
GACTACAAGG
TGTACGACGT
GCGGAAGATG
ATCGCCAAGA
GCGAGCAGGA
AATCGGCAAG
GCTACCGCCA
AGTACTTCTT
CTACAGCAAC
ATCATGAACT
TTTTCAAGAC
CGAGATTACC
CTGGCCAACG
GCGAGATCCG
GAAGGCGCCT
CTGATCGAGA
CAAACGGCGA
AACCGGGGAG
ATCGTGTGGG
ATAAGGGCCG
GGATTTTGCC
ACCGTGCGGA
AAGTGCTGAG
CATGCCCCAA
GTGAATATCG
TGAAAAAGAC
CGAGGTGCAG
ACAGGCGGCT
TCAGCAAAGA
GTCTATCCTG
CCCAAGAGGA
ACAGCGATAA
GCTGATCGCC
AGAAAGAAGG
ACTGGGACCC
TAAGAAGTAC
GGCGGCTTCG
ACAGCCCCAC
CGTGGCCTAT
TCTGTGCTGG
TGGTGGCCAA
AGTGGAAAAG
GGCAAGTCCA
AGAAACTGAA
GAGTGTGAAA
GAGCTGCTGG
GGATCACCAT
CATGGAAAGA
AGCAGCTTCG
AGAAGAATCC
CATCGACTTT
CTGGAAGCCA
AGGGCTACAA
AGAAGTGAAA
AAGGACCTGA
TCATCAAGCT
GCCTAAGTAC
TCCCTGTTCG
AGCTGGAAAA
CGGCCGGAAG
AGAATGCTGG
CCTCTGCCGG
CGAACTGCAG
AAGGGAAACG
AACTGGCCCT
GCCCTCCAAA
TATGTGAACT
TCCTGTACCT
GGCCAGCCAC
TATGAGAAGC
TGAAGGGCTC
CCCCGAGGAT
AATGAGCAGA
AACAGCTGTT
TGTGGAACAG
CACAAGCACT
ACCTGGACGA
GATCATCGAG
CAGATCAGCG
AGTTCTCCAA
GAGAGTGATC
CTGGCCGACG
CTAATCTGGA
CAAAGTGCTG
TCCGCCTACA
ACAAGCACCG
GGATAAGCCC
ATCAGAGAGC
AGGCCGAGAA
TATCATCCAC
CTGTTTACCC
TGACCAATCT
GGGAGCCCCT
GCCGCCTTCA
AGTACTTTGA
CACCACCATC
GACCGGAAGA
GGTACACCAG
CACCAAAGAG
GTGCTGGACG
CCACCCTGAT
CCACCAGAGC
ATCACCGGCC
TGTACGAGAC
ACGGATCGAC
CTGTCTCAGC
TGGGAGGCGA
CAAAAGGCCG
GCGGCCACGA
AAAAGGCCGG
CCAGGCAAAA
AAGAAAAAGG
AATTCGGCAG
TGGAGAGGGC
AGAGGAAGTC
TGCTAACATG
CGGTGACGTC
GAGGAGAATC
CTGGCCCAAT
GACCGAGTAC
AAGCCCACGG
TGCGCCTCGC
CACCCGCGAC
GACGTCCCCA
GGGCCGTACG
CACCCTCGCC
GCCGCGTTCG
CCGACTACCC
CGCCACGCGC
CACACCGTCG
ATCCGGACCG
CCACATCGAG
CGGGTCACCG
AGCTGCAAGA
ACTCTTCCTC
ACGCGCGTCG
GGCTCGACAT
CGGCAAGGTG
TGGGTCGCGG
ACGACGGCGC
CGCGGTGGCG
GTCTGGACCA
CGCCGGAGAG
CGTCGAAGCG
GGGGCGGTGT
TCGCCGAGAT
CGGCCCGCGC
ATGGCCGAGT
TGAGCGGTTC
CCGGCTGGCC
GCGCAGCAAC
AGATGGAAGG
CCTCCTGGCG
CCGCACCGGC
CCAAGGAGCC
CGCGTGGTTC
CTGGCCACCG
TCGGCGTCTC
GCCCGACCAC
CAGGGCAAGG
GTCTGGGCAG
CGCCGTCGTG
CTCCCCGGAG
TGGAGGCGGC
CGAGCGCGCC
GGGGTGCCCG
CCTTCCTGGA
GACCTCCGCG
CCCCACAACC
TCCCCTTCTA
CGAGCGGCTC
GGCTTCACCG
TCACCGCCGA
CGTCGAGGTG
CCCGAAGGAC
CGCGCACCTG
GTGCATGACC
CGCAAGCCCG
GTGCCTGAGA
ATTCTAACTA
GAGCTCGCTG
ATCAGCCTCG
ACTGTGCCTT
CTAGTTGCCA
GCCATCTGTT
GTTTGCCCCT
CCCCCGTGCC
TTCCTTGACC
CTGGAAGGTG
CCACTCCCAC
TGTCCTTTCC
TAATAAAATG
AGGAAATTGC
ATCGCATTGT
CTGAGTAGGT
GTCATTCTAT
TCTGGGGGGT
GGGGTGGGGC
AGGACAGCAA
GGGGGAGGAT
TGGGAAGAGA
ATAGCAGGCA
TGCTGGGGAG
CGGCCGCAGG
AACCCCTAGT
GATGGAGTTG
GCCACTCCCT
CTCTGCGCGC
TCGCTCGCTC
ACTGAGGCCG
GGCGACCAAA
GGTCGCCCGA
CGCCCGGGCT
TTGCCCGGGC
GGCCTCAGTG
AGCGAGCGAG
CGCGCAGCTG
CCTGCAGGGG
CGCCTGATGC
GGTATTTTCT
CCTTACGCAT
CTGTGCGGTA
TTTCACACCG
CATACGTCAA
AGCAACCATA
GTACGCGCCC
TGTAGCGGCG
CATTAAGCGC
GGCGGGTGTG
GTGGTTACGC
GCAGCGTGAC
CGCTACACTT
GCCAGCGCCC
TAGCGCCCGC
TCCTTTCGCT
TTCTTCCCTT
CCTTTCTCGC
CACGTTCGCC
GGCTTTCCCC
GTCAAGCTCT
AAATCGGGGG
CTCCCTTTAG
GGTTCCGATT
TAGTGCTTTA
CGGCACCTCG
ACCCCAAAAA
ACTTGATTTG
GGTGATGGTT
CACGTAGTGG
GCCATCGCCC
TGATAGACGG
TTTTTCGCCC
TTTGACGTTG
GAGTCCACGT
TCTTTAATAG
TGGACTCTTG
TTCCAAACTG
GAACAACACT
CAACCCTATC
TCGGGCTATT
CTTTTGATTT
ATAAGGGATT
TTGCCGATTT
CGGCCTATTG
GTTAAAAAAT
GAGCTGATTT
AACAAAAATT
TAACGCGAAT
TTTAACAAAA
TATTAACGTT
TACAATTTTA
TGGTGCACTC
TCAGTACAAT
CTGCTCTGAT
GCCGCATAGT
TAAGCCAGCC
CCGACACCCG
CCAACACCCG
CTGACGCGCC
CTGACGGGCT
TGTCTGCTCC
CGGCATCCGC
TTACAGACAA
GCTGTGACCG
TCTCCGGGAG
CTGCATGTGT
CAGAGGTTTT
CACCGTCATC
ACCGAAACGC
GCGAGACGAA
AGGGCCTCGT
GATACGCCTA
TTTTTATAGG
TTAATGTCAT
GATAATAATG
GTTTCTTAGA
CGTCAGGTGG
CACTTTTCGG
GGAAATGTGC
GCGGAACCCC
TATTTGTTTA
TTTTTCTAAA
TACATTCAAA
TATGTATCCG
CTCATGAGAC
AATAACCCTG
ATAAATGCTT
CAATAATATT
GAAAAAGGAA
GAGTATGAGT
ATTCAACATT
TCCGTGTCGC
CCTTATTCCC
TTTTTTGCGG
CATTTTGCCT
TCCTGTTTTT
GCTCACCCAG
AAACGCTGGT
GAAAGTAAAA
GATGCTGAAG
ATCAGTTGGG
TGCACGAGTG
GGTTACATCG
AACTGGATCT
CAACAGCGGT
AAGATCCTTG
AGAGTTTTCG
CCCCGAAGAA
CGTTTTCCAA
TGATGAGCAC
TTTTAAAGTT
CTGCTATGTG
GCGCGGTATT
ATCCCGTATT
GACGCCGGGC
AAGAGCAACT
CGGTCGCCGC
ATACACTATT
CTCAGAATGA
CTTGGTTGAG
TACTCACCAG
TCACAGAAAA
GCATCTTACG
GATGGCATGA
CAGTAAGAGA
ATTATGCAGT
GCTGCCATAA
CCATGAGTGA
TAACACTGCG
GCCAACTTAC
TTCTGACAAC
GATCGGAGGA
CCGAAGGAGC
TAACCGCTTT
TTTGCACAAC
ATGGGGGATC
ATGTAACTCG
CCTTGATCGT
TGGGAACCGG
AGCTGAATGA
AGCCATACCA
AACGACGAGC
GTGACACCAC
GATGCCTGTA
GCAATGGCAA
CAACGTTGCG
CAAACTATTA
ACTGGCGAAC
TACTTACTCT
AGCTTCCCGG
CAACAATTAA
TAGACTGGAT
GGAGGCGGAT
AAAGTTGCAG
GACCACTTCT
GCGCTCGGCC
CTTCCGGCTG
GCTGGTTTAT
TGCTGATAAA
TCTGGAGCCG
GTGAGCGTGG
AAGCCGCGGT
ATCATTGCAG
CACTGGGGCC
AGATGGTAAG
CCCTCCCGTA
TCGTAGTTAT
CTACACGACG
GGGAGTCAGG
CAACTATGGA
TGAACGAAAT
AGACAGATCG
CTGAGATAGG
TGCCTCACTG
ATTAAGCATT
GGTAACTGTC
AGACCAAGTT
TACTCATATA
TACTTTAGAT
TGATTTAAAA
CTTCATTTTT
AATTTAAAAG
GATCTAGGTG
AAGATCCTTT
TTGATAATCT
CATGACCAAA
ATCCCTTAAC
GTGAGTTTTC
GTTCCACTGA
GCGTCAGACC
CCGTAGAAAA
GATCAAAGGA
TCTTCTTGAG
ATCCTTTTTT
TCTGCGCGTA
ATCTGCTGCT
TGCAAACAAA
AAAACCACCG
CTACCAGCGG
TGGTTTGTTT
GCCGGATCAA
GAGCTACCAA
CTCTTTTTCC
GAAGGTAACT
GGCTTCAGCA
GAGCGCAGAT
ACCAAATACT
GTCCTTCTAG
TGTAGCCGTA
GTTAGGCCAC
CACTTCAAGA
ACTCTGTAGC
ACCGCCTACA
TACCTCGCTC
TGCTAATCCT
GTTACCAGTG
GCTGCTGCCA
GTGGCGATAA
GTCGTGTCTT
ACCGGGTTGG
ACTCAAGACG
ATAGTTACCG
GATAAGGCGC
AGCGGTCGGG
CTGAACGGGG
GGTTCGTGCA
CACAGCCCAG
CTTGGAGCGA
ACGACCTACA
CCGAACTGAG
ATACCTACAG
CGTGAGCTAT
GAGAAAGCGC
CACGCTTCCC
GAAGGGAGAA
AGGCGGACAG
GTATCCGGTA
AGCGGCAGGG
TCGGAACAGG
AGAGCGCACG
AGGGAGCTTC
CAGGGGGAAA
CGCCTGGTAT
CTTTATAGTC
CTGTCGGGTT
TCGCCACCTC
TGACTTGAGC
GTCGATTTTT
GTGATGCTCG
TCAGGGGGGC
GGAGCCTATG
GAAAAACGCC
AGCAACGCGG
CCTTTTTACG
GTTCCTGGCC
TTTTGCTGGC
CTTTTGCTCA
CATGT
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